CN110495318A - A kind of illumination method of regulation plants ' reproduction development - Google Patents

A kind of illumination method of regulation plants ' reproduction development Download PDF

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Publication number
CN110495318A
CN110495318A CN201910909858.2A CN201910909858A CN110495318A CN 110495318 A CN110495318 A CN 110495318A CN 201910909858 A CN201910909858 A CN 201910909858A CN 110495318 A CN110495318 A CN 110495318A
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illumination
regulation
periodicity
methods
plants
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CN110495318B (en
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李阳
张森
马健
罗梅秀
查萍
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Fujian Province Sino Science Biological Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

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  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention relates to technical field of plant illumination, more particularly to a kind of illumination method of regulation plants ' reproduction development, seed is subjected to vernalization, vegetable seedling after germination is subjected to continuous illumination using the first LED plant lamp, control the PPFD on vegetable seedling blade, the quantity of illumination is controlled, temperature and relative humidity are controlled, controls CO2Concentration;The excellent seedling of growing way is colonized, intermittent illumination is carried out to seedling using the 2nd LED plant lamp, controls the duty of intermittent light when illumination duration in a gap periodicity of illumination, control the PPFD on vegetable leaf, the quantity of illumination is controlled, temperature and relative humidity are controlled, controls CO2Concentration.The present invention luminous environment parameter and illumination methods different with the cultivation phase by nursery stage in regulation leaf vegetables breeding process, have effectively facilitated vegetables and have been converted from nutrient growth to reproductive growth, flowering time reduces 1/5th or more, and the period of collecting seed greatly shortens.

Description

A kind of illumination method of regulation plants ' reproduction development
Technical field
The present invention relates to technical field of plant illumination, and in particular to a kind of regulation plant Blooming shortens crop cycle Luminous environment regulates and controls method.
Background technique
After reproductive growth refers to plant growth to regular period, starts bud differentiation, and then yield positive results, form seed Process.Realize that the artificial regulatory of this life process is of great significance, especially agricultural production, as vegetable or flower is planted Training, crop breeding, people can control the growth cycle of crops, accelerate formation or the control Time To Market of fruit and seed, To generate huge economic benefit.
The process of plant growth and development is also illuminated by the light, temperature, root system nutrient and gas other than being controlled by autogene The influence of environment.Plant has the ability for accurately experiencing environmental signal, and can generate biochemical reaction rapidly in vivo, adjusts certainly The growth conditions of body, to adapt to the change of living environment.Light is both sole energy source and its life of photosynthesis of plant The important environmental signal of long development.Its medium wavelength of photosynthetically active radiation is the blood orange light of 610-700nm and the royal purple of 400-510nm Light is more absorbed by plant chlorophyll, causes the separation of charge and photochemical reaction of chlorophyll a, electronics is through a series of carrier Transmitting after for pushing the assimilation of CO2, provide material base for plant growth.Plant also passes through several light receptors and experiences and answer Reply miscellaneous and various luminous environment, such as phytochrome, image assesment, cryptochrome.These light receptors receive light quality, illumination respectively The dynamic-change information of intensity, photoperiod and direction of illumination carries out selection regulation after signal transduction, causes different photochemical Reaction is learned, plant is made to adapt to the dynamic change of luminous environment.With the continuous development of artificial light source, people can be by regulating and controlling illumination Intensity, photoperiod and spectral composition and illumination mode influence the growth and development of plant.Production in greenhouse and plant factor In, light is by the regulatory factor as a kind of efficient flowering of plant, result.
The special illumination form that intermittent illumination is artificially manufactured as one kind, there is important influence to plant growth and development. Some researchs find that the gap light of certain duty ratio and frequency can be used as a series of biochemistry in a kind of signal inducing plant body Reaction, to influence the energy utilization and carbon assimilation process of Photosynthetic.Wang Dafei is the study found that the cucumber of pulse light processing is young Seedling plant height, stem thickness, leaf area are noticeably greater than continuous light;Different frequency pulsed light can promote the accumulation of cucumber seedling substance;Different frequencies Rate pulsed light can dramatically increase cucumber seedling chlorophyll content.Wang Xiaoxu etc. has probed into LED light source different frequency and duty ratio pair The influence of Growth of Lettuce and its photosynthesis characteristics, in the day identical situation of amount of radiation, interval light processing is generally promoted for discovery Plant height, stem thickness and the leaf area of romaine lettuce.Olvera-Gonzalez etc. has found that the pulsed light of 0.1,1,100 and 1kHz can be improved The chlorophyll fluorescence parameters such as the Fv ' of tomato leaf/Fm ', NPQ, Φ PSII, ETR, Φ CO2, be eventually exhibited as yield increase and Capacity usage ratio improves.Relative to continuous illumination, under the pulsed light of low frequency, the photo-quantum efficiency of the Photosystem I I of tomato leaf is more Height, photosynthetic electron transfer rate can be reduced with the promotion of pulse light frequency.The researchs such as Dong Chen discovery is accounted for using 80% The growth result of continuous illumination may be implemented in the Millisecond intermittent light irradiation wheat of empty ratio, while photosynthetic rate can be improved.Liu Big equal tests discovery can promote inflorescences primordium differentiation using the dark intermittent mode of phase light, accelerate the formation of new PE curriculum inflorescence. " CN102124882A balsamine florescence control method ", using interruption illumination, controls its squaring period, increases number of blooming in the dark phase.Benefit It is shone with strobe light or interval light generating apparatus also has been reported that improve the biomass of plant, such as " CN207531418U one kind is based on The plant growth lighting device of stroboscopic ", " the illumination stroboscopic method and device of mono- plant growth of CN108184300A ", “EP1274665A1 METHOD OF CULTIVATING PLANT AND ILLUMINATOR FOR CULTIVATING PLANT”。
Light is both the sole energy source of photosynthesis of plant and the signal of its growth and development, and luminous environment is to plant Substance accumulation and form have vital effect, therefore how probe by luminous environment regulation crop fast-growth, bolting It blooms significant for saving planting time, raising reproductive efficiency with Seed Development.
In conclusion there is in the prior art using illumination stroboscopic method the quality of production for improving corresponding plant, Such as the stroboscopic illumination of the quality of production suitable for corresponding plant is provided come dynamic using the lighting device and stroboscopic method of design Scheme, primarily directed to the improvement of adaptive regulation method and device;In another example being discussed in a large amount of bibliography of above-mentioned reference Influence about the illumination using different frequency, different duty, varying strength to the growth result of different types of plant Difference, but be illustrated for how to improve yield and improve the content of micro elements by plants mostly in above-mentioned document, And how to be shortened by light control and plant a breeding cycle very perfect solution not yet.
Summary of the invention
In order to solve the above-mentioned technical problem, it is desirable to provide a kind of illumination method of regulation plants ' reproduction development realizes plant Blooming inoculation, can effectively shorten breeding cycle.
To achieve the above object, the present invention provides a kind of ring of light for shortening the vegetable breeding period by taking leaf vegetables plant as an example Border regulates and controls method, comprising the following steps:
(1) nursery stage
Seed is subjected to vernalization, the vegetable seedling after germination is subjected to Continuous irradiation, the first LED light using the first LED light Its spectral signature are as follows: the ratio between 600-700nm number of photons and 400-500nm number of photons are 2.0, are arranged on vegetable seedling blade PPFD reaches 150-200 μm of ol/m2/ s, control periodicity of illumination are 8-12 hour, control temperature and relative humidity be 15-20 DEG C with 90%, control CO2Concentration is 1000pm.
(2) phase is cultivated
When seedling reach can field planting standard after, the excellent young plant of growing way is colonized, using the second LED light carry out interval Irradiation, its spectral signature of the second LED light are as follows: the ratio between 600-700nm number of photons and 400-500nm number of photons are 3.8-3.9, setting PPFD on vegetable leaf is 200-300 μm of ol/m2The duty ratio of/s, the gap illumination are 10-50%, in a gap illumination The illumination duration is 0.1s-10s in period;The cumulative daylight that each processing is controlled by the way that suitable periodicity of illumination is arranged shines to measure 8-16mol/m2/ d, controls temperature and relative humidity is 22-23 DEG C and 80%, controls CO2Concentration is 1000pm.
It is different from the prior art, above-mentioned technical proposal has the advantages that
1. the present invention is for realizing the seedsetting of vegetables Blooming, at the same guarantee new seed bearing germination percentage with it is traditional continuous Illumination germination percentage is consistent.
2. the present invention is by vegetable in seedling period and LED plant lamp, no that difference number of photons used by the cultivation phase is distributed The setting such as same illumination methods (including continuous illumination and intermittent illumination), different illumination intensity, shortens the vegetable breeding period at least 20%.
3. the present invention carries out continuous illumination in nursery stage, to vegetable seedling, and controls blade intensity of illumination PPFD and illumination Period;In the cultivation phase, the young plant excellent to growing way carries out intermittent illumination, while illumination in duty ratio and a gap periodicity of illumination Duration, intensity of illumination PPFD value and periodicity of illumination, by under artificial light during vegetable breeding seedling stage and cultivation the phase not Same luminous environment parameter and illumination methods has effectively facilitated vegetables and has been converted from nutrient growth to reproductive growth, and flowering time shifts to an earlier date 1/5th or more, the period of collecting seed greatly shortens.
4. not only vegetable plant strain growth and development provides energy to intermittent light of the present invention, also continuous as a kind of signal Stimulate the photosensitive receptor of plant, induction plant generate substance and metamorphosis its reason may be illumination with light and shade alternately so that The photo flux density of unit time reduces, and plant accelerates transformation of the nutrient growth to reproductive growth because experiencing Low Light Density Stress.
Specific embodiment
Technology contents, construction feature, the objects and the effects for detailed description technical solution, below in conjunction with specific reality Example is applied to be explained in detail.
Embodiment 1
(1) nursery stage
By the sowing of romaine lettuce seed in the sponge that deionized water is soaked, then sponge is placed in constant temperature germinating box, it is black Dark situation, setting temperature are 20 DEG C, and relative humidity 90%, vernalization is taken out after 24 hours.Romaine lettuce after germination is placed into nursery Continue to cultivate on frame, the high 28cm of the nursery rack-layer, Continuous irradiation, spectral signature are as follows: 600-700nm light are carried out using LED light 1 The ratio between subnumber and 400-500nm number of photons are 2.0, and blade canopy intensity of illumination PPFD is 150 μm of ol/m2/s, and periodicity of illumination is every It 9 hours, temperature control was at 20-22 DEG C, relative humidity 80%.CO2 concentration 1000ppm.Every 2 days pouring one time of nutrition of nursery stage Liquid, the EC value of nutrient solution are 0.8-1.2ms/cm, pH 6.0-7.0.
(2) phase is cultivated
After sowing 20 days, by the excellent romaine lettuce seedling field planting to water planting cultivating stand of growing way, cultivating stand is equipped with adjustment height LED light 2, its spectral signature of the LED light are as follows: the ratio between 600-700nm number of photons and 400-500nm number of photons be 3.8, use LED light 2 carries out intermittent illumination.The following processing of setting:
T1: duty ratio 35% illumination duration 3s in the periodicity of illumination of gap, periodicity of illumination 21.5 hours;
T2: duty ratio 40% illumination duration 4s in the periodicity of illumination of gap, periodicity of illumination 20 hours;
T3: duty ratio 50% illumination duration 1s in the periodicity of illumination of gap, periodicity of illumination 15 hours;
T4: duty ratio 75% illumination duration 3s in the periodicity of illumination of gap, periodicity of illumination 10 hours;
CK: continuous illumination periodicity of illumination 7.5 hours;
Romaine lettuce canopy intensity of illumination is 200 μm of ol/m2/ s, and it is equal according to amount by the cumulative daylight that the photoperiod controls each processing For 8.1mol/m2/d.Temperature and relative humidity are controlled respectively in 22-23 DEG C and 80%, CO2Concentration control is used in 1000ppm The EC value of nutrient solution is 1.8-2.0ms/cm, PH 6.0-7.0.
(3) result and analysis
Conclusion: intermittent light T1, T2, T3 processing are compared with continuous illumination CK, without significant difference on percentage of seedgermination, but can be with To collect seed time shortening 22%.
Embodiment 2
(1) nursery stage
Spinach seed is put into after 55 DEG C of water seed soakings 2 hours and is soaked seed 22 hours under room temperature, is sowed in deionization water logging It in wet sponge, is put into constant temperature germinating box, dark surrounds, setting temperature is 15 DEG C, relative humidity 90%, to most of kind It takes out and is seeded in the sponge that deionized water is soaked after son dew bud, vernalization is taken out after 120 hours, is placed and is continued to train on seedling culture frame It supports, the high 28cm of the nursery rack-layer, Continuous irradiation, its spectral signature of LED light 1 are as follows: 600-700nm number of photons is carried out using LED light 1 It is 2.0 with the ratio between 400-500nm number of photons, blade canopy intensity of illumination PPFD is 200 μm of ol/m2/s, periodicity of illumination daily 12 Hour, temperature control is at 18-20 DEG C, relative humidity 80%.CO2 concentration 1000ppm.Every 2 days pouring one time of nutrition liquid of nursery stage, The EC value of nutrient solution is 0.8-1.2ms/cm, pH 6.0-7.0
(2) phase is cultivated
After sowing 15 days, by the excellent romaine lettuce seedling field planting to water planting cultivating stand of growing way, cultivating stand is equipped with adjustment height LED light 3, spectral signature are as follows: the ratio between 600-700nm number of photons and 400-500nm number of photons be 3.9, using LED light 3 into Row intermittent illumination.The following processing of setting:
T1: duty ratio 40% illumination duration 3s in the periodicity of illumination of gap, periodicity of illumination 22.5 hours;
T2: duty ratio 45% illumination duration 9s in the periodicity of illumination of gap, periodicity of illumination 20 hours;
T3: duty ratio 50% illumination duration 1s in the periodicity of illumination of gap, periodicity of illumination 18 hours;
T4: duty ratio 75% illumination duration 3s in the periodicity of illumination of gap, periodicity of illumination 12 hours;
CK: continuous illumination periodicity of illumination 9 hours;
Romaine lettuce canopy intensity of illumination is 300 μm of ol/m2/s, and equal according to amount by the cumulative daylight that the photoperiod controls each processing For 8.1mol/m2/d.Temperature and relative humidity are controlled respectively in 22-23 DEG C and 80%, CO2Concentration control is used in 1000ppm The EC value of nutrient solution is 1.8-2.0ms/cm, PH 6.0-7.0.
(3) result and analysis
By Experimental comparison, it is concluded that intermittent light T1, T2, T3 processing are compared with continuous illumination CK, on percentage of seedgermination Without significant difference, but the time that can will collect seed shortens 20%.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or the terminal device that include a series of elements not only include those Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or end The intrinsic element of end equipment.In the absence of more restrictions, being limited by sentence " including ... " or " including ... " Element, it is not excluded that there is also other elements in process, method, article or the terminal device for including the element.This Outside, herein, " being greater than ", " being less than ", " being more than " etc. are interpreted as not including this number;" more than ", " following ", " within " etc. understand Being includes this number.
Although the various embodiments described above are described, once a person skilled in the art knows basic wounds The property made concept, then additional changes and modifications can be made to these embodiments, so the above description is only an embodiment of the present invention, It is not intended to limit scope of patent protection of the invention, it is all to utilize equivalent structure or equivalent made by present specification Process transformation, being applied directly or indirectly in other relevant technical fields, similarly includes in patent protection model of the invention Within enclosing.

Claims (9)

1. a kind of illumination methods of regulation plants ' reproduction development, which is characterized in that in growing process, at least use gap Illumination methods realize that plant Blooming produces seeds, and the duty ratio of the gap illumination is 10-50%, in a gap periodicity of illumination The interior illumination duration is 0.1s-10s.
2. the illumination methods of regulation plants ' reproduction development according to claim 1, which is characterized in that in nursery stage using company Continue illumination methods, spectral signature in luminous environment are as follows: the ratio between 600-700nm number of photons and 400-500nm number of photons are 2.0.
3. the illumination methods of regulation plants ' reproduction development according to claim 3, which is characterized in that nursery stage intensity of illumination For 150-200 μm of ol/m2/ s, periodicity of illumination 9-12h/d.
4. the illumination methods of regulation plants ' reproduction development according to claim 3, which is characterized in that vegetables are arranged in nursery stage PPFD in seedling leaves reaches 150-200 μm of ol/m2/ s, control periodicity of illumination are 8-12 hour, control temperature and relatively wet Degree is 15-20 DEG C and 90%, controls CO2Concentration is 1000pm.
5. the illumination methods of regulation plants ' reproduction development according to claim 1, which is characterized in that continuous illumination mode is Continuous irradiation is carried out using the first LED light.
6. the illumination methods of regulation plants ' reproduction development according to claim 1, which is characterized in that between the cultivation phase uses It has a rest illumination methods, spectral signature in luminous environment are as follows: the ratio between 600-700nm photon accounting and 400-500nm photon accounting are 3.8- 3.9。
7. the illumination methods of regulation plants ' reproduction development according to claim 7, which is characterized in that the cultivation phase illumination Intensity is 200-300 μm of ol/m2/ s, periodicity of illumination 8-24h/d.
8. the illumination methods of regulation plants ' reproduction development according to claim 7, which is characterized in that intermittent illumination mode is Intermittent irradiation is carried out using the second LED light.
9. the illumination methods of regulation plants ' reproduction development according to claim 7, which is characterized in that
It is 200-300 μm of ol/m that the PPFD on vegetable leaf, which is arranged, in the cultivation phase2/ s, the duty ratio of the gap illumination are 10-50%, The illumination duration is 0.1s-10s in a gap periodicity of illumination, and control cumulative daylight is 8-16mol/m according to amount2/ d, control Temperature and relative humidity processed are 22-23 DEG C and 80%, control CO2Concentration is 1000pm.
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CN112868419A (en) * 2019-12-01 2021-06-01 广州清凉农业科技有限公司 Agricultural sunlight transmission illumination system, greenhouse matched with same and illumination method
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