KR102138276B1 - Method for producing ginseng with increased ginsenoside content in plant factory - Google Patents

Abstract

The present invention relates to a method for preparing ginseng with an increased content of ginsenoside Rg1 by irradiating an artificial light source at a frequency of 20 to 30 Hz in a plant factory, and to a food composition containing ginseng and the ginseng prepared by the above manufacturing method. , Since ginseng with an increased content of Rg1 can be produced, it is very useful in the pharmaceutical and food industry using ginseng.

Description

Method for producing ginseng with increased ginsenoside content in plant factory}

The present invention relates to a method for producing ginseng in which the content of ginsenoside Rg1 is increased by irradiating an artificial light source of a specific frequency in a plant factory, ginseng prepared thereby, and a food composition comprising the ginseng.

Plant factories can efficiently produce crops regardless of external environmental factors in a situation where crops are suffering a lot from recent climate change. As an artificial light source for plant cultivation, LED is easy to install in a small space, has a long lifespan, and can be intermittently irradiated in minutes or seconds, so it is economical because it consumes less power than other light sources. In addition, since it is possible to select the light quality of a specific wavelength, it can perform functions such as promoting photosynthesis, controlling flowering, and increasing sugar content and saponin. In order to produce high value-added crops through the production method of a plant factory, it is necessary to apply various additional environmental stresses that can increase the quality and content of functional ingredients in an appropriate cultivation system that can secure productivity. Among these stresses, controlling the light environment is the most effective way to increase production and increase the content of functional materials.

As a prior art, in a closed plant factory system that promotes the growth of spinach by adjusting the light intensity and photoperiod of the LED lamp wavelength of red: blue: white in a ratio of 8:1:1. There is a document (Korean Patent Publication No. 2015-0121925) on the spinach cultivation method.

In addition, there is a document (Korean Patent Publication No. 2013-0023303) on a method of cultivation of black pepper in a closed plant factory system through control of the nutrient solution EC, light intensity, and planting distance. However, concepts such as frequency (Hz) and duty ratio are not included in light qualities such as luminance and photoperiod disclosed in the above-described document.

Since the duty ratio has an effect on power saving, studies on plant reactions accordingly are in progress, but studies on plant reactions to frequency (Hz) are insufficient.

Korean Patent Publication No. 2015-0121925 Korean Patent Publication No. 2013-0023303

One object of the present invention is to provide a method for producing ginseng in which the content of ginsenoside Rg1 is increased, comprising the step of irradiating an artificial light source at a frequency of 20 to 30 Hz in a plant factory.

Another object of the present invention is to provide ginseng prepared by the method for producing ginseng.

Another object of the present invention is to provide a food composition containing the ginseng.

To achieve the above purpose, the content of ginseng, Rg1, in ginseng grown by irradiating an artificial light source at a different frequency at a plant factory is measured, and ginseng grown at 25 Hz is stronger than ginseng grown at other frequency bands. The present invention was completed by confirming that the content of cenoside Rg1 was high.

In order to achieve the above object, the present invention provides a method for producing ginseng with an increased content of ginsenoside Rg1, including the step of irradiating an artificial light source at a frequency of 20 to 30 Hz in a plant factory.

The term "plant factory" of the present invention refers to a system that produces agricultural products throughout the year, such as producing industrial products using advanced technologies such as environmental control and automation, and is completely blocked from the outside and crops with artificial lighting alone. It is divided into a full-control type for cultivating cultivation and a combined solar type for cultivating crops using both sunlight and artificial lighting indoors such as a greenhouse. In other words, plant factories control the environmental conditions such as temperature, light, CO2, and culture medium that affect the growth of crops in an optimal state, and automate the work process to produce crops in the facility regardless of weather conditions. it means.

The plant factory of the present invention preferably uses a fully controlled plant factory to control the wavelength band, frequency, luminosity, duty ratio, photoperiod, temperature or humidity conditions of the irradiated light.

The term ginseng (Panax ginseng CA Mayer) of the present invention is a plant belonging to the genus Araliaceae ginseng botanically, has been used for medicinal purposes since BC in China, and has been used for trade and medicinal purposes in Korea since the Three Kingdoms period, Even today, it is widely used as a herbal medicine or health food in various fields.

The ginsenoside of the present invention is classified into a triol series and a diol series according to a structure in which sugars are attached to ginseng saponin, and the triol series protopanaxatriol (PPT) is There are Re, Rf, Rg1, Rg2, Rh1, etc., and the diol-based protopanaxadiol (PPD) is Ra1, Ra2, Ra3, Rb1, Rb2, Rb3, Rc, Rd, Rs, Rg3, Rg5. , Rh2, etc.

Ginsenoside Rg1, a kind of triol-based ginsenoside, is known to have a function of relieving fatigue, improving memory learning function, and promoting DNA and RNA synthesis, and is used as an index component of ginseng products and red ginseng products. The structure is the same as in Formula 1.

In the present invention, the artificial light source means an artificial device that supplies light so that ginseng can photosynthesize, and generally, various types of light emitting means such as resistance bulbs, discharge lamps, and LEDs (Light-Emitting Diode) can be used. , Specifically, LED (Light-Emitting Diode) can be used, and more specifically, any one or more selected from blue light, red light, yellow light, far red light, white light, and a mixed light LED (Light-Emitting Diode) thereof can be used. have. In addition, the frequency of the artificial light source may be 20 to 30 Hz, specifically 25 Hz.

The term LED (Light-Emitting Diode) of the present invention is a semiconductor device used to send and receive signals by converting an electrical signal into infrared or light using the characteristics of a compound semiconductor, and has a longer lifespan than other luminous bodies and uses a lower voltage. At the same time, it has the advantage of low power consumption and has excellent response speed and impact. LEDs produced in recent years are energy efficient, have a much higher total photon flux density than incandescent lamps in the 400-700nm range, and can produce wavelengths with a higher total photon flux density than incandescent lamps for specific wavelengths. have.

The term irradiation of the present invention refers to shining light on a surface, and means exposing light from an artificial light source to the surface of a plant.

In one embodiment of the present invention, in order to check the frequency band of artificial light that increases the content of ginsenoside Rg1 during ginseng cultivation in a plant factory, one ginseng grown for 100 days by varying the frequency of the artificial light source from 25 to 2500 Hz. The content of ginsenoside Rg1 was measured.

As a result of the measurement, the content of ginsenoside Rg1 of ginseng grown at 60 to 2500 Hz was measured to be 0.25 to 0.87 mg/g, but the content of ginsenoside Rg1 of ginseng grown at 25 Hz was measured to be 0.121 mg/g. , It was confirmed that ginseng grown at 25Hz has a higher content of ginsenoside Rg1 than ginseng grown at other frequencies.

It was confirmed that ginseng grown at 25Hz has a higher ginsenoside Rg1 content than ginseng grown in other frequency bands, and ginseng with increased ginsenoside Rg1 content by irradiating an artificial light source at a frequency of 20 to 30㎐ at a plant factory. Can be manufactured.

In the embodiment of the present invention, ginseng was prepared using a duty ratio of 30% of a light source in a plant factory.

Therefore, the present invention may be a method for producing ginseng, characterized in that the duty ratio of the artificial light source is 25 to 35%.

The duty ratio of the present invention is a term used for a pulse having a period, and the duty ratio of the present invention means an on duty ratio. This means the ratio of the time the pulse is on for one period. Therefore, when the duty ratio is lowered, the off state is lengthened, and thus power consumption can be reduced.

Accordingly, the method of manufacturing ginseng according to the present invention may be a method of manufacturing ginseng in which the duty ratio is 20 to 30%, and the power consumption is low, thereby reducing manufacturing cost.

In one embodiment of the present invention, red light of 630 to 660 nm and blue light of 440 nm in a plant factory were mixed in a ratio of 11:7 to be used.

In the present invention, red light refers to light having a wavelength of 620 to 700 nm, and blue light refers to light having a wavelength of 430 to 480 nm, specifically, the wavelength of red light is 620 to 670 nm, and the wavelength of the blue light is 430 to It may be 450 nm, more specifically, the red light may be 630 to 660 nm, and the blue light may be 440 nm.

Accordingly, the present invention may be a method for producing ginseng, wherein the artificial light source comprises red light having a wavelength of 620 to 670 nm and blue light having a wavelength of 430 to 450 nm in a ratio of 10 to 12:5 to 8. It may be a method for producing ginseng, characterized in that the red light and the blue light are in a ratio of 11:7.

In one embodiment of the present invention, the artificial light source is irradiated with light at 53.87 μmol m ^-2 s ^-1 light intensity for 16 hours (specify), and blocking the light for 6 hours (memorization) is periodically performed. Ginseng was repeatedly prepared.

Accordingly, the present invention may be a method for producing ginseng, characterized in that the luminous intensity of the artificial light source is 43 to 63 μmol m ^-2 s ^-1, and the light intensity and the memorization period are irradiated with light with a photoperiod of 14 to 18:6 to 10.

The term "luminosity" of the present invention means a photosynthetic photon flux density (PPFD), and means the amount of photons falling per unit time per unit area. The plant factory of the present invention uses an artificial light source of 43 to 63 μmol m ^-2 s ^-1 , and specifically 53.87 μmol m ^-2 s ^-1 .

The higher the luminosity, the more the growth of plants is promoted, but the production cost of ginseng may increase due to a large amount of electricity consumption.

The term “description” of the present invention refers to the time when the ginseng is irradiated with the light of the artificial light source in the plant factory, and memorization refers to the time to block the light in the plant factory. The photoperiod means that the memorization and the memorization intersect and repeat at a certain time, and the time excluding the memorization is based on 24 hours (a day). In the present invention, the specified period may be 14 hours to 18 hours, and specifically 16 hours. Therefore, the memorization may be 6 to 10 hours, specifically 8 hours.

In the embodiment of the present invention, during the cultivation period of ginseng, the internal temperature of the plant factory was maintained at 22 to 25°C and the relative humidity at 75 to 85%.

Therefore, the present invention may be a method for producing ginseng, characterized in that the internal temperature of the plant factory is maintained at a temperature of 22 to 25°C and a relative humidity of 75 to 85%.

In another aspect, the present invention provides ginseng prepared by the method for producing ginseng.

The ginseng was manufactured by irradiating an artificial light source at a frequency of 20 to 30 Hz in a plant factory, and the content of ginsenoside Rg1 is higher than that of ginseng produced in other wavelength bands, and the content of ginsenoside Rg1, an index of ginseng products. It can be a high-quality ginseng.

The terms plant factory, artificial light source, and ginsenoside Rg1 of the present invention are as described above.

In another aspect, the present invention provides a food composition containing the ginseng.

The food composition of the present invention may include the form of pills, powders, granules, needles, tablets, capsules or liquids, and the type of food to which ginseng with an increased content of ginsenoside Rg1 of the present invention can be added There are no specific restrictions, for example, various beverages, chewing gum, tea, vitamin complexes, and dietary supplements.

In addition to ginseng having increased ginsenoside Rg1, other ingredients may be added to the food composition, and the kind is not particularly limited. For example, it may contain as an additional component, such as various herbal extracts, food additives or natural carbohydrates that are acceptable for food, as in ordinary foods, but is not limited thereto.

In the present invention, the term "food supplementary additive" refers to a component that can be added auxiliary to food, and is added to the manufacture of health functional foods of each formulation, and can be appropriately selected and used by those skilled in the art. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavoring agents, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc. are included, but the types of the food additives of the present invention are not limited by the above examples.

Examples of the natural carbohydrate include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; And polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, etc.), stevia extract (rebaudioside A, glysir) Hibiscus) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used advantageously.

The food composition of the present invention may contain health functional food. The term "health functional food" as used in the present invention refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills using raw materials or ingredients having useful functions for the human body. Here, the term "functionality" refers to obtaining useful effects for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and at the time of the production, it can be prepared by adding raw materials and ingredients commonly added in the art. In addition, unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time by using food as a raw material, and it may be excellent in portability.

The mixing amount of the active ingredient can be appropriately determined according to the purpose of use. In general, the ginseng with increased ginsenoside Rg1 of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 5 to 10% by weight of the raw material composition, but is not limited thereto. . However, in the case of long-term intake for the purpose of health and hygiene or for health control purposes, the amount may be used even below the above range.

There is no particular limitation on the type of food. Examples of foods to which the above substances can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, There are alcoholic beverages and vitamin complexes, and all health functional foods in the usual sense are included.

The present invention relates to a method for producing ginseng with an increased content of ginsenoside Rg1 by irradiating an artificial light source at a frequency of 20 to 30 Hz in a plant factory, ginseng prepared by the above manufacturing method, and a food composition containing the ginseng. As a result, it is possible to manufacture high value-added ginseng with an increased Rg1 content, so it is very useful in the pharmaceutical and food industries using ginseng.

1 is a schematic diagram of an experiment of a plant factory according to an embodiment of the present invention.
2 is a schematic cross-sectional view of a chamber of a plant factory according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail by examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

<Material>

Soil in a ratio of 1:1 between top and sand was placed in a white plastic pot and one-year-old ginseng individuals were transplanted.

<cultivation of ginseng>

The pot to which the ginseng was transplanted prepared above was cultivated for 100 days in a chamber in a plant factory.

As an artificial light source of the plant factory, an LED light source in which red light of 630 to 660 nm wavelength and 440 nm blue light were mixed was used, and the mixing ratio of red light and blue light was irradiated at a ratio of 11:7, and the temperature inside the plant factory during the cultivation period. Was maintained at 22 to 25°C, and the relative humidity was maintained at 75 to 85%

In order to compare the change in the content of metabolites of ginseng according to the change in the frequency of the light source irradiated during ginseng cultivation, the ginsengs of Comparative Examples 1 to 5 and Examples were cultivated by varying the frequency of the light source to 25 to 25000 Hz.

Comparative Example 1 (60 Hz)

For the cultivation of ginseng, a red and blue mixed LED light source was used, and during the cultivation period, the light was irradiated with a duty ratio of 30%, 60Hz, 53.87μmol m ^-2 s ^-1 light, 16 hours light period, and 8 hours memory period.

Comparative Example 2 (180 Hz)

For ginseng cultivation, a red and blue mixed LED light source was used, and during the cultivation period, the light was irradiated with a duty ratio of 30%, 180Hz, 53.87μmol m ^-2 s ^-1 luminous intensity, 16 hours light period, and 8 hours memory period.

Comparative Example 3 (540 Hz)

For ginseng cultivation, a red and blue mixed LED light source was used, and during the cultivation period, the light was irradiated with a duty ratio of 30%, 540Hz, 53.87μmol m ^-2 s ^-1 luminous intensity and 16 hours light period and 8 hours memorization period.

Comparative Example 4 (1620 Hz)

For ginseng cultivation, a red and blue mixed LED light source was used, and during the cultivation period, the light was irradiated with a duty ratio of 30%, 1620Hz, 53.87μmol m ^-2 s ^-1 luminous intensity, 16 hours light period, and 8 hours memorization period.

Comparative Example 5 (25000 ㎐)

For ginseng cultivation, a red and blue mixed LED light source was used, and during the cultivation period, the light was irradiated with a duty ratio of 30%, 25000Hz, 53.87μmol m ^-2 s ^-1 luminous intensity, 16 hours light period, and 8 hours memorization period.

Example (25 Hz)

For the cultivation of ginseng, a red and blue mixed LED light source was used, and during the cultivation period, the light was irradiated with a duty ratio of 30%, 25Hz, 53.87μmol m ^-2 s ^-1 light, 16 hours light period, and 8 hours memory period.

<Experimental Example> Analysis of the content of metabolites of ginseng

In order to confirm the change in the metabolite content of ginseng according to the frequency change of the artificial light source irradiated during ginseng cultivation in a plant factory, the ginseng of Comparative Examples 1 to 5 and Examples were used as an HPLC system (Agilent Technolgies, USA). The content of Rg1, a kind of metabolite used as an indicator, was measured.

Sample name (frequency) Rg1 content (mg/g) Comparative Example 1
(60Hz) 0.030 Comparative Example 2
(180Hz) 0.025 Comparative Example 3
(540Hz) 0.049 Comparative Example 4
(1620Hz) 0.087 Comparative Example 5
(25000Hz) 0.072 Example
(25Hz) 0.121

Table 1 is a table showing the content of final metabolites of ginseng according to the mineral quality in the plant factory.

The content of Rg1 of ginseng of Comparative Examples 1 to 5 was measured from 0.25 to 0.87 mg/g, but the content of Rg1 of ginseng grown at 25 Hz of Example was measured to be 0.121 mg/g, compared to Comparative Examples 1 to 5 at 25 Hz. It can be seen that the content of Rg1 is high.

Therefore, according to the present invention, light having a wavelength of 11:7 ratio of red and blue light through an LED light source in a plant factory is used at a duty ratio of 30%, 25Hz, 53.87μmol m ^-2 s ^-1 and 16 It was confirmed that the content of the metabolite Rg1 of ginseng was increased when cultivated by irradiation with light in the period specified in time and in the photoperiod of 8 hours memorization.

The above examples are for illustrative purposes only, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not construed as being limited by these examples. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the above description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (7)

Including the step of irradiating an artificial light source with a duty ratio of 30%, a frequency of 25 Hz, and a luminous intensity of 53.87 μmolm ^-2 s ^-1 in a plant factory,
The artificial light source consists of a ratio of 10 to 12:5 to 8 of red light with a wavelength of 620 to 670 nm and blue light with a wavelength of 430 to 450 nm,
The internal temperature of the plant factory is maintained at a temperature of 22 to 25 °C and a relative humidity of 75 to 85%,
A method for producing ginseng with an increased content of ginsenoside Rg1. delete delete The method of claim 1,
The method of manufacturing ginseng, characterized in that irradiating light with a photoperiod of 14-18: 6-10 in the bright and memorized period of the artificial light source. delete Ginseng manufactured by the method for manufacturing ginseng of claim 1. A food composition containing the ginseng of claim 6.

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