CN103518623A - Fast breeding method for strawberry seedlings - Google Patents
Fast breeding method for strawberry seedlings Download PDFInfo
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- CN103518623A CN103518623A CN201310501884.4A CN201310501884A CN103518623A CN 103518623 A CN103518623 A CN 103518623A CN 201310501884 A CN201310501884 A CN 201310501884A CN 103518623 A CN103518623 A CN 103518623A
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Abstract
The invention belongs to a non-tube rapid propagation technology for plants, and relates to a fast breeding method for strawberry seedlings. The method comprises the following steps: A, selection and preprocessing of a in vitro material, namely, selecting inter-stem buds in buds on stolons of the strawberry seedlings, sterilizing notches with 5 carbendazim, and then soaking with naphthylacetic acid; B, colonization of the in vitro material, namely, inserting the in vitro material into a perlite matrix; C, rooting stage management of the in vitro material, namely, controlling the growth environmental condition of the plants through an automatic control system, spraying a nutrient solution once every 24 hours beginning from bourgeon of new roots, and eye-surveying the length of the new roots of 3-5 cm; D, training seedling stage management of the in vitro material, namely, detecting the upper part of the in vitro material to grow 1-2 leaves, and then entering a seedling stage; and E, seedling stage management, namely, transplanting the seedlings to cultivation containers or cultivation ground beds with conventional strawberry cultivation soil, and growing into commercial seedlings after 15-20 days. By using the method provided by the invention, the breeding speed and breeding survival rate are greatly increased, and the technology is more suitable for industrial implementation of large-scale fast seedling cultivation.
Description
Technical field
The invention belongs to the non-tube rapid propagation technology of plant, relate to a kind of fast breeding method of Strawberry Seedlings.
Background technology
Existing Strawberry Seedlings mating system, mainly contains tissue culture technique and traditional branch cutting technology.But the cutting propagation cycle reaches 60～75 days conventionally, cutting propagation survival rate is 60～75%, and is difficult to realize and grows seedlings on a large scale.Tissue culture technique is used for cultivating nontoxic seedling (generation seedling), is only applicable to scientific experiment, and cost is high, is difficult to large-scale production.
Summary of the invention
Technical problem to be solved by this invention is, provides a kind of and can increase substantially the speed of breeding and breed survival rate, and be applicable to the fast breeding method of the Strawberry Seedlings of growing seedlings on a large scale.
The fast breeding method of Strawberry Seedlings of the present invention comprises the following steps:
Selected and the pretreatment of A, To body material: choose bud between stem on Strawberry Seedlings stolon in sprouting, band 1-2 sheet leaf, otch is level and smooth, the carbendazim sterilization of 50～1000 times of dilute with waters for otch, then soak 10～15 minutes with the methyl α-naphthyl acetate of 50～1000 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 3000～4000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～28 ℃ of matrix and air themperatures, matrix relative moisture is 65～70%, relative air humidity is 70～80%; From new root eruption, every 24 hours spray nutritious liquors 1 time (potassium dihydrogen phosphate, mass concentration 5 ‰-6 ‰), estimate new root length 3～5cm, enter the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 3000～4000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～28 ℃ of matrix and air themperatures, matrix relative moisture is 70～75%, relative air humidity is 75～85%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional cultivated strawberry soil,
conventional cultivation management 1within 5～20 days, become commercial seedling.
The present invention compares and has the following advantages with existing Strawberry Seedlings raising technology:
1, increased substantially the speed of breeding and bred survival rate.Through reality, implement test, foreshorten to 20-30 days breeding cycle; By the cutting propagation survival rate 60～75% of prior art, bring up to more than 98%.
2, be applicable to growing seedlings on a large scale.The present invention can control rearing condition by intelligence control system, compare with the artificial cutting propagation of prior art, realize automation, intellectuality, precision and the high efficiency management of seedling raising process, be more applicable to the industrialized implementation of extensive fast seedling growing.
Embodiment
The fast breeding method of the Strawberry Seedlings of the embodiment of the present invention comprises the following steps:
Selected and the pretreatment of A, To body material: on Strawberry Seedlings stolon, in sprouting, choose bud between stem, band 1-2 sheet leaf, otch is level and smooth, the carbendazim sterilization of 1000 times of dilute with waters for otch, then soak 15 minutes with the methyl α-naphthyl acetate of 500 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 3500Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 24 ℃, night, temperature was 19 ℃, and matrix relative moisture is 65%, relative air humidity is 70%; From new root eruption, every 24 hours spray nutritious liquors 1 time (potassium dihydrogen phosphate, mass concentration 5 ‰), estimate new root length 3～5cm, enter the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 3500Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 26 ℃, and night, temperature was 19 ℃, and matrix relative moisture is 70%, relative air humidity is 75%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional cultivated strawberry soil,
conventional cultivation management 1within 5～20 days, become commercial seedling.
The Strawberry Seedlings of embodiment of the present invention cultivation, through test, 20-30 days breeding cycles, breeds survival rate more than 98%.
Claims (4)
1. the fast breeding method of a Strawberry Seedlings comprises the following steps:
Selected and the pretreatment of A, To body material: choose bud between stem on Strawberry Seedlings stolon in sprouting, band 1-2 sheet leaf, otch is level and smooth, the carbendazim sterilization of 50～1000 times of dilute with waters for otch, then soak 10～15 minutes with the methyl α-naphthyl acetate of 50～1000 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 3000～4000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～28 ℃ of matrix and air themperatures, matrix relative moisture is 65～70%, relative air humidity is 70～80%; From new root eruption, within every 24 hours, spray nutritious liquor is 1 time, estimates new root length 3～5cm, enters the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 3000～4000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～28 ℃ of matrix and air themperatures, matrix relative moisture is 70～75%, relative air humidity is 75～85%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional cultivated strawberry soil,
conventional cultivation management 1within 5～20 days, become commercial seedling.
2. the fast breeding method of Strawberry Seedlings according to claim 1, is characterized in that: the nutrient solution in step C and D is the potassium dihydrogen phosphate of mass concentration 5 ‰-6 ‰.
3. the fast breeding method of Strawberry Seedlings according to claim 1, is characterized in that: in step C, it is intensity of illumination 3500Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 26 ℃, night, temperature was 19 ℃, and matrix relative moisture is 65%, relative air humidity is 70%.
4. the fast breeding method of Strawberry Seedlings according to claim 1, is characterized in that: it is intensity of illumination 3500Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 26 ℃, and night, temperature was 19 ℃, and matrix relative moisture is 70%, relative air humidity is 75%.
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| CN201310501884.4A CN103518623A (en) | 2013-10-23 | 2013-10-23 | Fast breeding method for strawberry seedlings |
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| CN201310501884.4A CN103518623A (en) | 2013-10-23 | 2013-10-23 | Fast breeding method for strawberry seedlings |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108713486A (en) * | 2018-05-30 | 2018-10-30 | 中国农业大学 | Strawberry Water culture method for culturing seedlings under a kind of artificial light environment |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5921589A (en) * | 1982-07-28 | 1984-02-03 | 三菱化学株式会社 | Strawberry culturing method |
| CN102577825A (en) * | 2012-03-17 | 2012-07-18 | 常熟市尚湖农业生态园有限公司 | High-yield and pollution-free cultivation method for strawberries |
| JP2012139154A (en) * | 2010-12-28 | 2012-07-26 | National Agriculture & Food Research Organization | Method for promoting floral bud differentiation of strawberry |
| CN103070002A (en) * | 2013-02-05 | 2013-05-01 | 镇江市丹徒区紫杉生态农业园 | Schefflera octophylla non-test tube rapid propagation method |
| CN103238454A (en) * | 2013-05-30 | 2013-08-14 | 管朝来 | Strawberry planting method |
| CN103329802A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Pollution-free high-yield planting method for strawberries |
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2013
- 2013-10-23 CN CN201310501884.4A patent/CN103518623A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5921589A (en) * | 1982-07-28 | 1984-02-03 | 三菱化学株式会社 | Strawberry culturing method |
| JP2012139154A (en) * | 2010-12-28 | 2012-07-26 | National Agriculture & Food Research Organization | Method for promoting floral bud differentiation of strawberry |
| CN102577825A (en) * | 2012-03-17 | 2012-07-18 | 常熟市尚湖农业生态园有限公司 | High-yield and pollution-free cultivation method for strawberries |
| CN103070002A (en) * | 2013-02-05 | 2013-05-01 | 镇江市丹徒区紫杉生态农业园 | Schefflera octophylla non-test tube rapid propagation method |
| CN103238454A (en) * | 2013-05-30 | 2013-08-14 | 管朝来 | Strawberry planting method |
| CN103329802A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Pollution-free high-yield planting method for strawberries |
Non-Patent Citations (2)
| Title |
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| SHIGEHIKO HAYASHI ET.AL.,: "Development of Circulating-Type Movable Bench System for Strawberry Cultivation", 《JARQ》 * |
| 邓雪印: "日光温室草莓优质高效栽培技术", 《果农之友》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108713486A (en) * | 2018-05-30 | 2018-10-30 | 中国农业大学 | Strawberry Water culture method for culturing seedlings under a kind of artificial light environment |
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Application publication date: 20140122 |