CN110463604A - A kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link - Google Patents
A kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link Download PDFInfo
- Publication number
- CN110463604A CN110463604A CN201810436796.3A CN201810436796A CN110463604A CN 110463604 A CN110463604 A CN 110463604A CN 201810436796 A CN201810436796 A CN 201810436796A CN 110463604 A CN110463604 A CN 110463604A
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- China
- Prior art keywords
- seedling
- culture
- sugarcane
- transplanting
- tissue
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention discloses a kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link, it cultivated for 1~2 generation the following steps are included: sugar-cane tissue culture seedlings are first in strong seedling culture base, then carries out culture of rootage；Tissue culture seedling rooting metaplexus is planted in nursery 30~40 days in Seedlings nursery, when every plant of sugarcane Miao Jun has independent root system, to plant division after the appropriate hardening of clump cultivation seedling progress；Naked seedling single plant is directly transplanted to ready seedling breeding crop field；Survival rate management is improved after cultivation.Sugarcane tissue-culture-seedling bare-seedling method for transplanting of the invention can effectively overcome the problems, such as that the time is long, occupancy nursery place area is big, production cost is high etc. is cultivated in the preceding nursery in sugar-cane tissue culture seedlings plantation crop field.
Description
Technical field
The present invention relates to plant tissue culture technical fields, move more particularly to a kind of sugarcane tissue-culture-seedling bare-seedling without temporary planting link
Cultivating method.
Background technique
In order to accelerate new good and Virus-free seedcane the popularization reproduction speed of sugarcane, using advanced Plant Tissue Breeding skill
Art carries out cane seedling fast numerous.Sugar-cane tissue culture seedlings extremely transplant crop field this period in bottle seedling of taking root at present, generally by nursery
Sand bed clump plants, divides the links such as the temporary planting of single plant nutrition cup, field-transplanting, has larger adverse effect to the breeding and popularization of seedling, main
Show themselves in that one, single plant nutrition cup temporary planting nursery occupy the nursery time it is long, low efficiency the conditions such as illumination, temperature and moisture compared with
4 good~October, nutrition cup heeled-in seedling, which is planted from plant division to being out of the garden, needs 30~40 day time, and temporary planting nutritive cup seedlings are occupied
Area of nursery bed is larger, is 4 times or so that clump plants seedling area；Two, the plant of seedling cost high nutrition cup seed, nursery bed management, be out of the garden
Work need to put into a large amount of material resources, labour, and transport inconvenient, high production cost, and the comprehensive average seedling cost of every young plant reaches
0.2 yuan or so；Three, the time due to tissue-cultured seedling in nursery is long, delay transplanting, opposite to shorten grown in field time, yield drop
It is low, it is unfavorable for that breeding tissue culture sprout quick is numerous and promotion rate.
Summary of the invention
It is sweet effectively to overcome the object of the present invention is to provide a kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link
Sugarcane tissue-cultured seedling plants the problem of preceding nursery in crop field cultivates that the time is long, occupies that nursery place area is big, production cost is high etc., raising group
Train seedling unit area seedling stem yield.
The scheme that the present invention solves technical problem is: a kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link, it
The following steps are included:
1) sugar-cane tissue culture seedlings first cultivated for 1~2 generation in strong seedling culture base, then carried out culture of rootage；
2) tissue culture seedling rooting metaplexus is planted in nursery 30~40 days in Seedlings nursery, when every plant of sugarcane Miao Jun has independent root system, to clump
It plants seedling and carries out plant division after appropriate hardening；
3) naked seedling single plant is directly transplanted to ready seedling breeding crop field；
4) survival rate management is improved after planting.
In step 1), the strong seedling culture base is free from the MS minimal medium of hormone；1~2 generation of the cultivation, it is
Incubation time, 1 about 15~20 days time in generation.
In step 2, it is that will take root bottle seedling after outdoor the culture of carry out hardening 3~7 days, by bottle seedling of taking root that the clump, which is planted,
It removes outside bottle, every bottle seedling is divided into several clumps, then 3~5 plants of seedlings of every Cong Miao are planted on seedbed；The Seedlings nursery is that have
The preferable seedling growth greenhouse of water proof wind proof light transmission, seedling medium are mainly sterile new soil or river sand；It is described that seedling progress is planted suitably to clump
Hardening, be to stop fertilising, and gradually decrease the application of water, after 5~6 days, stop leaching when sugarcane seedling vacation stem is up to 15 centimetres or so
Water 2~3 days, plant division was carried out when symptom occurs slightly suffering from drought in sugarcane seedling；The plant division is that the clump Jing Guo hardening is planted to seedling from seedbed
It pulls up, then every young plant is individually separated, prepare transplanting crop field.
In step 3), the naked seedling direct transplantation is that single plant seedling is cut off 2/3 blade, yellow with ABT3 root-inducing powder is contained
Mud starches root, is then colonized in crop field by certain planting density, drenches sufficient root water.
In step 4), survival rate management is improved after the cultivation, is tissue culture root-naked transplanting, be colonized latter all left and right sugarcane seedlings at
After work, work into normal field management.
The invention has the advantages that: sugarcane tissue-culture-seedling bare-seedling method for transplanting of the invention can effectively overcome sugarcane
Tissue-cultured seedling plants the problem of preceding nursery in crop field cultivates that the time is long, occupies that nursery place area is big, production cost is high etc..The present invention and
Lifting is easy, convenient transportation, is extended the grown in field time, unit area seedling stem yield 25% or so be can be improved, per hectare can increase
6400 yuan of the output value or so, remarkable in economical benefits.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail:
Sugarcane tissue-culture-seedling bare-seedling method for transplanting of the invention, it the following steps are included:
1) sugar-cane tissue culture seedlings first cultivated for 2 generations in strong seedling culture base, then carried out culture of rootage；
2) tissue culture seedling rooting metaplexus is planted in nursery 40 days in Seedlings nursery, when every plant of sugarcane Miao Jun has independent root system, plants seedling to clump
Carry out plant division after appropriate hardening；
3) naked seedling single plant is directly transplanted to ready seedling breeding crop field；
4) survival rate management is improved after planting.
In step 1), the strong seedling culture base is free from the MS minimal medium of hormone；2 generations of the cultivation, for culture
Time, 1 time in generation are 18 days.
In step 2, it is that will take root bottle seedling after outdoor the culture of carry out hardening 6 days that the clump, which is planted, and the bottle seedling that will take root is moved
Outside bottle outlet, every bottle seedling is divided into several clumps, then 4 plants of seedlings of every Cong Miao are planted on seedbed；The Seedlings nursery is with waterproof
The windproof preferable seedling growth greenhouse of light transmission, seedling medium are mainly sterile new soil or river sand；It is described that seedling progress refining appropriate is planted to clump
Seedling is when sugarcane seedling vacation stem is up to 15 centimetres or so, and stopping is applied fertilizer, and gradually decreases the application of water, after 6 days, stops water drenching 2 days,
Plant division is carried out when symptom occurs slightly suffering from drought in sugarcane seedling；The plant division is that the clump Jing Guo hardening is planted seedling to pull up from seedbed, then
Every young plant is individually separated, transplanting crop field is prepared.
In step 3), the naked seedling direct transplantation is that single plant seedling is cut off 2/3 blade, yellow with ABT3 root-inducing powder is contained
Mud starches root, is then colonized in crop field by certain planting density, drenches sufficient root water.
In step 4), survival rate management is improved after the cultivation, is tissue culture root-naked transplanting, be colonized latter all left and right sugarcane seedlings at
After work, work into normal field management.
Sugarcane tissue-culture-seedling bare-seedling method for transplanting of the invention can effectively overcome the preceding nursery in sugar-cane tissue culture seedlings plantation crop field to cultivate
The problem of time is long, occupies that nursery place area is big, production cost is high etc..Of the invention and lifting is easy, convenient transportation, is extended big
Unit area seedling stem yield 25% or so can be improved in field growth time, and per hectare can increase production 6400 yuan of value or so, and economic benefit is aobvious
It writes.
Claims (5)
1. it is a kind of without temporary planting link sugarcane tissue-culture-seedling bare-seedling method for transplanting, it is characterised in that: it the following steps are included:
1) sugar-cane tissue culture seedlings first cultivated for 1~2 generation in strong seedling culture base, then carried out culture of rootage；
2) tissue culture seedling rooting metaplexus is planted in nursery 30~40 days in Seedlings nursery, when every plant of sugarcane Miao Jun has independent root system, to clump
It plants seedling and carries out plant division after appropriate hardening；
3) naked seedling single plant is directly transplanted to ready seedling breeding crop field；
4) survival rate management is improved after planting.
2. a kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link according to claim 1, it is characterised in that: In
In step 1), the strong seedling culture base is free from the MS minimal medium of hormone；1~2 generation of the cultivation, be incubation time, 1
For the time about 15~20 days.
3. a kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link according to claim 1, it is characterised in that: In
In step 2, it is that will take root bottle seedling after outdoor the culture of carry out hardening 3~7 days that the clump, which is planted, and the bottle seedling that will take root removes outside bottle,
Every bottle seedling is divided into several clumps, then 3~5 plants of seedlings of every Cong Miao are planted on seedbed；The Seedlings nursery is with water proof wind proof
The preferable seedling growth greenhouse of light transmission, seedling medium are mainly sterile new soil or river sand；It is described that seedling progress hardening appropriate is planted to clump, be
When sugarcane seedling vacation stem is up to 15 centimetres or so, stopping is applied fertilizer, and gradually decreases the application of water, after 5~6 days, stops water drenching 2~3 days,
Plant division is carried out when symptom occurs slightly suffering from drought in sugarcane seedling；The plant division is that the clump Jing Guo hardening is planted seedling to pull up from seedbed, then
Every young plant is individually separated, transplanting crop field is prepared.
4. a kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link according to claim 1, it is characterised in that: In
In step 3), the naked seedling direct transplantation is that single plant seedling is cut off 2/3 blade, starches root with containing ABT3 root-inducing powder yellow mud, so
It is colonized in crop field by certain planting density afterwards, drenches sufficient root water.
5. a kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link according to claim 1, it is characterised in that: In
In step 4), survival rate management is improved after the cultivation, is tissue culture root-naked transplanting, after the latter all left and right sugarcane seedling of field planting survives, enter
Normal field management work.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810436796.3A CN110463604A (en) | 2018-05-09 | 2018-05-09 | A kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810436796.3A CN110463604A (en) | 2018-05-09 | 2018-05-09 | A kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link |
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| CN110463604A true CN110463604A (en) | 2019-11-19 |
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| CN201810436796.3A Pending CN110463604A (en) | 2018-05-09 | 2018-05-09 | A kind of sugarcane tissue-culture-seedling bare-seedling method for transplanting without temporary planting link |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111727687A (en) * | 2020-06-30 | 2020-10-02 | 广西壮族自治区农业科学院 | Cultivation method of sugarcane transplanted seedlings |
| CN113545268A (en) * | 2021-08-13 | 2021-10-26 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
-
2018
- 2018-05-09 CN CN201810436796.3A patent/CN110463604A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111727687A (en) * | 2020-06-30 | 2020-10-02 | 广西壮族自治区农业科学院 | Cultivation method of sugarcane transplanted seedlings |
| CN113545268A (en) * | 2021-08-13 | 2021-10-26 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
| CN113545268B (en) * | 2021-08-13 | 2022-09-09 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
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Application publication date: 20191119 |