CN110521566A - A kind of luminous environment regulation method of regulation plant metabolism substance - Google Patents

A kind of luminous environment regulation method of regulation plant metabolism substance Download PDF

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Publication number
CN110521566A
CN110521566A CN201910909834.7A CN201910909834A CN110521566A CN 110521566 A CN110521566 A CN 110521566A CN 201910909834 A CN201910909834 A CN 201910909834A CN 110521566 A CN110521566 A CN 110521566A
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China
Prior art keywords
illumination
light
intermittent
vegetables
periodicity
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CN201910909834.7A
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Chinese (zh)
Inventor
李阳
陈艺群
马健
罗梅秀
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Fujian Province Sino Science Biological Co Ltd
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Fujian Province Sino Science Biological Co Ltd
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Priority to CN201910909834.7A priority Critical patent/CN110521566A/en
Publication of CN110521566A publication Critical patent/CN110521566A/en
Priority to PCT/CN2020/114158 priority patent/WO2021057476A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • GPHYSICS
    • G05CONTROLLING; REGULATING
    • G05DSYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
    • G05D27/00Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
    • G05D27/02Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

Abstract

The present invention relates to technical field of plant illumination, regulate and control method more particularly to a kind of luminous environment of regulation plant metabolism substance, the complete artificial continuous illumination of tradition and at least one intermittent illumination of addition are used in plant culture each stage, and cooperate other growing environments, the good aubergine vegetables of coloring can be quickly obtained, it is again not in the underproduction, while the content of the anthocyanidin content in aubergine vegetables, vitamin C and soluble protein can be promoted again, the quality of aubergine vegetables is further improved, the condition and flavor of aubergine vegetables are improved.

Description

A kind of luminous environment regulation method of regulation plant metabolism substance
Technical field
The present invention relates to technical field of plant illumination, and in particular to a kind of luminous environment regulation side of regulation plant metabolism substance Method.
Background technique
Plant intracellular metabolite activity is divided into nascent metabolism and secondary metabolism, occurs in all plants, it is green to be related to leaf The life necessary material such as element, sugar, amino acid, fatty acid, nucleic acid is metabolized as nascent metabolism;Occur in Activities of Some Plants body, relates to And some pairs of plant life nonessential materials are metabolized as secondary metabolism.Secondary metabolite is plant during long-term evolution With the interaction of its living environment as a result, main includes the small molecular organic compounds such as alkaloid, flavones, terpene and saponin(e, It is generated and distribution usually has kind, organ, tissue and the specificity in growth and development period.Generation caused by different plants Substance, especially secondary metabolites are thanked, value is had important application to fields such as human lives, productions.Such as medicinal plant Secondary metabolites is many kinds of, and structure is totally different, so far it has been found that flavonoids, phenols, cumarin, lignin, alkaloid, glucosides, Terpene, steroid, saponin(e, polyacetylene, organic acid etc..Majority has antitumor, anticancer, anti-oxidant, reduction blood lipid and cholesterol Effect.Artemisinine in sweet wormwood has the effect of anti-malarial, antitumor, antimycotic, immunological regulation;Anthocyanidin in vegetables can also Enough enhance blood vessel elasticity, improve the circulatory system and promote the smoothness of skin, inhibits inflammation and allergy, improve the flexible of joint Property.
Purple (red) color vegetables contain anthocyanidin, and anthocyanidin is a kind of water colo(u)r being widely present in plant, belong to Flavonoids and the main color-generation substance of plant are the next of the colors such as red, purple, aubergine, blue in plant Source.Anthocyanidin is hydroxyl donor, while being also a kind of free radical scavenger, it can be combined with protein prevents peroxidating.Cyanine Element is potent antioxidant, can maintain normal cell linkages, the stabilization of blood vessel, enhancement fine vascular circulation, improve micro- blood The flowing of pipe and vein.Therefore, edible purple food of arranging in pairs or groups becomes trend, and especially purple (red) color vegetables are increasingly consumed The favor of person.
Currently, the cultivation low output of this purple or the vegetable variety of red, especially under Protected Cultivation, such as people In Gong Guang plant factor, some purple (red) color vegetable varieties, with the extension of plant strain growth phase, color can gradually taking off, cyanine Cellulose content is insufficient, is not able to satisfy the demand in market.The principal element of influence Anthocyanin content accumulation has following: cyanine The influence of plain construction, nitrogen source, potash fertilizer, pH, temperature, illumination, carbon source, the inside and outside factor and cultivation technique etc..Up to now, also have Correlative study scholar is studied for the coloring of luminous environment aubergine vegetables.Fan Yinglun et al. is studies have shown that increase UV-A The content of anthocyanidin in potato can be improved with feux rouges.Wang Zhidong et al. using short wave ultraviolet UV-C studies have shown that handled The content of fresh-cut violet cabbage anthocyanidin can be improved.Qi Yan et al. is studies have shown that UV-A and UV-B processing can improve cabbage seedling Anthocyanidin content.Above researcher shows, and using ultraviolet light wave band treatment with irradiation, can be conducive to vegetable flower blueness cellulose content Accumulation may be higher due to ultraviolet light wave band lamps and lanterns manufacturing cost but in the specific practical application of plant factor, and Ultraviolet light adversely affects the staff in plant factor, therefore in the large scale application of plant factor, uses There are obstacles for ultraviolet light wave band.The accumulation for also having document report blue light to be conducive to aubergine vegetables anthocyanidin simultaneously, Zhan Liying are ground Study carefully discovery, blue light can promote the accumulation of purple cabbage leaves anthocyanidin.Patent 201610014069.9 proposes to harvest in aubergine Later period uses blue light instead and is handled, and may be implemented to colour, but yield can be decreased obviously, while effect is unobvious.Also part grinds Study carefully scholar may be implemented to colour, but coloring degree is limited, energy consumption in the aubergine romaine lettuce harvesting later period using continuous illumination mode Cost increases.
In conclusion since aubergine vegetable growth is slow, anthocyanidin content is insufficient, even if harvesting the later period in aubergine vegetables It uses continuous illumination mode instead and realizes coloring, but since coloring degree is limited and yield can be decreased obviously.Therefore suitable light is needed Environment conditioning technology both can guarantee that the coloring in harvesting of aubergine vegetables preferably, improved anthocyanidin content, while the not underproduction, special It is not the industrialized planting for realizing aubergine vegetables under plant factor's facility condition, is that those skilled in the art are urgently to be resolved Technical problem.
Summary of the invention
In order to solve the above-mentioned technical problem, it is desirable to provide a kind of luminous environment regulation method of regulation plant metabolism substance needs Improving anthocyanidin content enhances leaf color, while not occurring the underproduction and then promoting the luminous environment regulation method of quality of vegetable.
The present invention provides a kind of luminous environments of regulation plant metabolites to regulate and control method, adopts in plant culture each stage With traditional complete artificial continuous illumination and add at least one intermittent illumination.
Further, the intermittent light is the intermittent light of two or more light quality, and intermittent light radiation modality is to intersect Irradiation or non-crossing irradiation.
Further, in cross-fire mode, the duty ratio < 50% of intermittent light.
Further, in non-crossing radiation modality, duty ratio >=50% of intermittent light.
Further, intermittent illumination light quality is monochromatic spectrum or Quan Guangpu, and illumination continues in a gap periodicity of illumination Time is 1s-20s, and periodicity of illumination is≤for 24 hours/d.
Further, the intermittent illumination is ultra-blue-light 450nm intermittent illumination.
Further, continuous illumination light quality is Quan Guangpu, periodicity of illumination 8-12h/d.
Further, continuous illumination and intermittent illumination light intensity are 200-300 μm of ol/ ㎡ s.
To achieve the above object, the present invention also provides the cropping patterns under above-mentioned full human controllable's environment to plant Training, the specific steps are as follows:
(1) seeding and seedling raising
After aubergine vegetable seeds is carried out clear water seed soaking, it is multicast in sponge square, 1, every cave;Place it in germinating box Middle carry out vernalization carries out nursery processing, nursery stage every each 2 days pouring one time of nutrition liquid, nutrient solution EC value after seed shows money or valuables one carries unintentionally For 0.8-1.2ms/cm, pH 6.0-7.0;
(2) culturing and planting
When vegetable seedling length to 4-5 piece true leaf, culturing and planting is carried out under illumination condition, vegetables are moved on into field planting plate On, field planting line space is 15cm × 20cm, environmental condition are as follows: diurnal temperature maintains 20-23 DEG C/18-20 DEG C, and air is relatively wet Degree is 60-70%, using continuous illumination, intensity of illumination 200-300 μm of ol/ ㎡ s, periodicity of illumination 8-12h/d;As vegetables are raw Nutrient solution EC value is gradually increased for long-term extension, and the EC value of nutrient solution is 1.5-2.0ms/cm, PH 6.0-7.0, nutrient solution Temperature is 20-22 DEG C, dissolved oxygen amount 5-6mg/L;
(3) it harvests
Within the last week picking time, using the side of the full spectrum continuous illumination of LED and the combination of ultra-blue-light 450nm intermittent illumination Formula or in such a way that the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination combine, colours vegetables.Between The duty ratio that occult light shines is 25-90%, preferably 50-90%.The illumination duration is 1s-20s in a gap periodicity of illumination. The intermittent illumination photoperiod is≤for 24 hours/d, and the continuous illumination photoperiod is 8-12h/d. continuous illumination and intermittent illumination light intensity is 200- 300 μm of ol/ ㎡ s handle vegetables 2-7d using this technology, can be quickly obtained aubergine vegetables, anthocyanidin, vitamin C contain Amount and content of soluble protein greatly increase, while yield is not reduced.
Be different from the prior art, the present invention have it is following the utility model has the advantages that
(1) present invention is to the side for during aubergine vegetable growth, acquiring tradition continuous illumination and intermittent illumination combines Formula efficiently realizes the fast colorizing of aubergine vegetables, improves the content of the beneficial metabolics substance such as anthocyanidin, vitamin C, mentions High quality of vegetable, while in turn avoiding the appearance of underproduction problem.
(2) in step (3), the vegetables within one week picking time of adjusting the distance carry out between monochrome 450nm blue light the present invention Occult light irradiation, irradiation can be realized the fast colorizing of aubergine vegetables for 2-7 days, compare traditional dye technology, high-efficient, effect Obviously, operating procedure is simple, is convenient for industrial applications;
(3) present invention cultivates aubergine vegetables using the controllable luminous environment of full artificial light, is the life of aubergine vegetables Length provides optimal temperature, light, water, fertilizer etc., and the cultivation of aubergine vegetables is made to get rid of temperature, region and the limitation in season.
Specific embodiment
Technology contents, construction feature, the objects and the effects for detailed description technical solution, below in conjunction with specific reality It applies example and attached drawing is cooperated to be explained in detail.
Embodiment 1
The present embodiment 1 uses purple U.S. romaine lettuce as sample, and specific incubation step is as follows:
(1) seeding and seedling raising
Purple U.S. romaine lettuce seed is chosen, after purple U.S. romaine lettuce seed is carried out clear water seed soaking, is multicast in sponge square, every cave one Grain;Then there is the sponge block of purple U.S. romaine lettuce seed to be placed in germinating box sowing again and carry out vernalization, after seed shows money or valuables one carries unintentionally, using water Training carries out nursery processing, and the nursery stage, the EC value for controlling nutrient solution was 0.8ms/cm, and pH is per each 2 days pouring one time of nutrition liquid 6.0-7.0;
(2) culturing and planting
When purple U.S. romaine lettuce length to 4-5 piece true leaf, select on the purple U.S. romaine lettuce seedling replanting to field planting plate of neat and consistent, it is fixed Plant line space is 15cm × 20cm, and the U.S. vegetables of purple after field planting are put into nutrition liquid bath and are cultivated, are planted using nutrient film Training technology, the EC value of nutrient solution are 1.5ms/cm, and PH 6.0-7.0, solution temperature is 20-22 DEG C, dissolved oxygen amount 5-6mg/ L.Ambient temperature conditions are as follows: day temperature maintains 20-23 DEG C, and night temperatures maintain 18-20 DEG C, and relative air humidity is 60-70%, with the extension of vegetable growth phase, during entire culturing and planting is gradually increased in nutrient solution EC value, illumination condition is adopted With the full spectrum lamp of LED, intensity of illumination is 250 μm of ol/m2S, periodicity of illumination 10h;
(3) it harvests
By the U.S. romaine lettuce of purple after field planting 24d, using the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination knot The mode of conjunction, and the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination intersect progress, colour to vegetables, try It is as follows to test group processing scheme:
The full spectrum of LED: duty ratio 37.5%, the illumination duration is 6s in the periodicity of illumination of gap, periodicity of illumination 16 hours, 200 μm of ol/ ㎡ s of intensity of illumination;
Ultra-blue-light: duty ratio 37.5%, the illumination duration is 6s in the periodicity of illumination of gap, periodicity of illumination 16 hours, 200 μm of ol/ ㎡ s of intensity of illumination;
3d is handled, is harvested.
1 processing scheme of control group is as follows:
The full spectrum of LED: continuous illumination mode, 200 μm of ol/m of intensity of illumination2S, photoperiod 12h handle 3d, carry out Harvesting.
2 processing scheme of control group is as follows:
Ultra-blue-light: continuous illumination mode, 200 μm of ol/m of intensity of illumination2S, photoperiod 12h handle 3d, are adopted It receives.
3 processing scheme of control group is as follows:
Full spectrum+the ultra-blue-light of LED: continuous illumination mode, the full spectral illumination of LED, 200 μm of ol/m of intensity of illumination2S, Photoperiod is 6h;Carrying out ultra-blue-light irradiation, 200 μm of ol/m of intensity of illumination2S, photoperiod 6h handle 3d, are adopted It receives.
(4) result and analysis
After harvesting, 50 plants of purple U.S. romaine lettuce are randomly selected, to its ground fresh weight, anthocyanidin content, Vitamin C content and can Dissolubility albumen measures, and is averaged, and result is as shown in the table:
As a result: embodiment 1 at least improves 20% than 2 anthocyanidin content of control group, and ground fresh weight is improved than control group 1 1.0%;
Embodiment 2
The present embodiment 2 uses red Luo Sha romaine lettuce as sample, and specific incubation step is as follows:
(1) seeding and seedling raising
Red Luo Sha romaine lettuce seed is chosen, after red Luo Sha romaine lettuce seed is carried out clear water seed soaking, is multicast in sponge square, every cave One;Then there is the sponge block of red Luo Sha romaine lettuce seed to be placed in germinating box sowing again to carry out vernalization, after seed shows money or valuables one carries unintentionally, adopt Nursery processing is carried out with water planting, the nursery stage, the EC value for controlling nutrient solution was 1.0ms/cm per each 2 days pouring one time of nutrition liquid, PH is 6.0-7.0;
(2) culturing and planting
When red Luo Sha romaine lettuce seedling length to 4-5 piece true leaf, the red Luo Sha romaine lettuce seedling replanting of neat and consistent is selected to calmly It plants on plate, field planting line space is 15cm × 20cm, the red Luo Sha romaine lettuce after field planting is put into nutrition liquid bath and is cultivated, nutrition The EC value of liquid is 1.8ms/cm, and PH 6.0-7.0, solution temperature is 20-22 DEG C, dissolved oxygen amount 5-6mg/L.Environment temperature item Part are as follows: day temperature maintains 20-23 DEG C, and night temperatures maintain 18-20 DEG C, relative air humidity 60-70%, with vegetable The extension in dish growth period, during entire culturing and planting is gradually increased in nutrient solution EC value, illumination condition uses the full spectrum lamp of LED, Intensity of illumination is 250 μm of ol/m2S, periodicity of illumination 8h;
(3) it harvests
By the red Luo Sha romaine lettuce after field planting 20d, using the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination In conjunction with mode, and the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination intersect and carry out, and colour to vegetables, Test group processing scheme is as follows:
The full spectrum of LED: duty ratio 40%, the illumination duration is 12s in the periodicity of illumination of gap, and periodicity of illumination 12.5 is small When, 250 μm of ol/ ㎡ s of intensity of illumination;
Ultra-blue-light: duty ratio 40%, the illumination duration is 12s in the periodicity of illumination of gap, periodicity of illumination 12.5 hours, 250 μm of ol/ ㎡ s of intensity of illumination;
2d is handled, is harvested.
4 processing scheme of control group is as follows:
The full spectrum of LED: continuous illumination mode, 250 μm of ol/m of intensity of illumination2S, photoperiod 10h handle 2d, carry out Harvesting.
5 processing scheme of control group is as follows:
Ultra-blue-light: continuous illumination mode, 250 μm of ol/m of intensity of illumination2S, photoperiod 10h handle 2d, are adopted It receives.
6 processing scheme of control group is as follows:
Full spectrum+the ultra-blue-light of LED: continuous illumination mode, the full spectral illumination of LED, 250 μm of ol/m of intensity of illumination2S, Photoperiod is 5h;Carrying out ultra-blue-light irradiation, 250 μm of ol/m of intensity of illumination2S, photoperiod 5h handle 2d, are adopted It receives.
(4) result and analysis
After harvesting, 50 plants of red Luo Sha romaine lettuce are randomly selected, to its ground fresh weight, anthocyanidin content and Vitamin C content It measures, is averaged, result is as shown in the table:
As a result: embodiment 3 at least improves 10% than 4 anthocyanidin content of control group, and ground fresh weight is improved than control group 4 2.8%;
Embodiment 3
The present embodiment 3 uses red wrinkle romaine lettuce as sample, and specific incubation step is as follows:
(1) seeding and seedling raising
Red wrinkle romaine lettuce seed is chosen, after red wrinkle romaine lettuce seed is carried out clear water seed soaking, is multicast in sponge square, every cave one Grain;Then there is the sponge block of red wrinkle romaine lettuce seed to be placed in germinating box sowing again and carry out vernalization, after seed shows money or valuables one carries unintentionally, using water Training carries out nursery processing, and the nursery stage, the EC value for controlling nutrient solution was 1.2ms/cm, and pH is per each 2 days pouring one time of nutrition liquid 6.0-7.0;
(2) culturing and planting
When red wrinkle romaine lettuce length to 4-5 piece true leaf, select on the red wrinkle romaine lettuce seedling replanting to field planting plate of neat and consistent, it is fixed Plant line space is 15cm × 20cm, and the red wrinkle romaine lettuce after field planting is put into nutrition liquid bath and is cultivated, is planted using nutrient film Training technology, the EC value of nutrient solution are 1.2-2.0ms/cm, and PH 6.0-7.0, solution temperature is 20-22 DEG C, dissolved oxygen amount 5- 6mg/L.Ambient temperature conditions are as follows: day temperature maintains 20-23 DEG C, and night temperatures maintain 18-20 DEG C, relative air humidity For 60-70%, with the extension of vegetable growth phase, during entire culturing and planting is gradually increased in nutrient solution EC value, illumination condition Using the full spectrum lamp of LED, intensity of illumination is 300 μm of ol/m2S, periodicity of illumination 12h;
(3) it harvests
By the red wrinkle romaine lettuce after field planting 23d, using the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination knot The mode of conjunction, and the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination intersect progress, colour to vegetables, try It is as follows to test group processing scheme:
The full spectrum of LED: duty ratio 25%, the illumination duration is 3s in the periodicity of illumination of gap, periodicity of illumination 24 hours, light According to 300 μm of ol/ ㎡ s of intensity;
Ultra-blue-light: duty ratio 25%, the illumination duration is 3s in the periodicity of illumination of gap, periodicity of illumination 24 hours, light According to 300 μm of ol/ ㎡ s of intensity;
4d is handled, is harvested.
7 processing scheme of control group is as follows:
The full spectrum of LED: continuous illumination mode, 300 μm of ol/m of intensity of illumination2S, photoperiod 12h handle 4d, carry out Harvesting.
8 processing scheme of control group is as follows:
Ultra-blue-light: continuous illumination mode, 300 μm of ol/m of intensity of illumination2S, photoperiod 12h handle 4d, are adopted It receives.
(4) result and analysis
After harvesting, randomly select 50 plants of red wrinkle romaine lettuce, to its ground fresh weight, anthocyanidin content and Vitamin C content into Row measurement, is averaged, result is as shown in the table:
As a result: embodiment 3 at least improves 15% than 8 anthocyanidin content of control group, and ground fresh weight is improved than control group 7 1.6%;
Embodiment 4
The present embodiment 4 uses purple U.S. romaine lettuce as sample, and specific incubation step is as follows:
(1) seeding and seedling raising
Purple U.S. romaine lettuce seed is chosen, after purple U.S. romaine lettuce seed is carried out clear water seed soaking, is multicast in sponge square, every cave one Grain;Then there is the sponge block of purple U.S. romaine lettuce seed to be placed in germinating box sowing again and carry out vernalization, after seed shows money or valuables one carries unintentionally, using water Training carries out nursery processing, and the nursery stage, the EC value for controlling nutrient solution was 0.8ms/cm, and pH is per each 2 days pouring one time of nutrition liquid 6.0-7.0;
(2) culturing and planting
When purple U.S. romaine lettuce length to 4-5 piece true leaf, select on the purple U.S. romaine lettuce seedling replanting to field planting plate of neat and consistent, it is fixed Plant line space is 15cm × 20cm, and the U.S. vegetables of purple after field planting are put into nutrition liquid bath and are cultivated, are planted using nutrient film Training technology, the EC value of nutrient solution are 1.5ms/cm, and PH 6.0-7.0, solution temperature is 20-22 DEG C, dissolved oxygen amount 5-6mg/ L.Ambient temperature conditions are as follows: day temperature maintains 20-23 DEG C, and night temperatures maintain 18-20 DEG C, and relative air humidity is 60-70%, with the extension of vegetable growth phase, during entire culturing and planting is gradually increased in nutrient solution EC value, illumination condition is adopted With the full spectrum lamp of LED, intensity of illumination is 250 μm of ol/m2S, periodicity of illumination 10h;
(3) it harvests
By the U.S. romaine lettuce of purple after field planting 24d, using the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination knot The mode of conjunction, and the full spectrum intermittent illumination of LED first irradiates, ultra-blue-light 450nm intermittent illumination irradiates again again, non-crossing photograph It penetrates, vegetables is coloured, test group processing scheme is as follows:
The full spectrum of LED: duty ratio 75%, the illumination duration is 6s in the periodicity of illumination of gap, periodicity of illumination 8 hours, light According to 200 μm of ol/ ㎡ s of intensity;
Ultra-blue-light: duty ratio 75%, the illumination duration is 6s in the periodicity of illumination of gap, periodicity of illumination 8 hours, illumination 200 μm of ol/ ㎡ s of intensity;
3d is handled, is harvested.
9 processing scheme of control group is as follows:
The full spectrum of LED: continuous illumination, 200 μm of ol/m of intensity of illumination2S, photoperiod 12h handle 3d, are harvested.
10 processing scheme of control group is as follows:
Ultra-blue-light: continuous illumination, 200 μm of ol/m of intensity of illumination2S, photoperiod 12h handle 3d, are harvested.
(4) result and analysis
After harvesting, 50 plants of purple U.S. romaine lettuce are randomly selected, to its ground fresh weight, anthocyanidin content, Vitamin C content and can Dissolubility albumen measures, and is averaged, and result is as shown in the table:
As a result: embodiment 4 at least improves 16% than 10 anthocyanidin content of control group, and ground fresh weight is improved than control group 9 1.1%;
Embodiment 5
The present embodiment 5 uses red Luo Sha romaine lettuce as sample, and specific incubation step is as follows:
(1) seeding and seedling raising
Red Luo Sha romaine lettuce seed is chosen, after red Luo Sha romaine lettuce seed is carried out clear water seed soaking, is multicast in sponge square, every cave One;Then there is the sponge block of red Luo Sha romaine lettuce seed to be placed in germinating box sowing again to carry out vernalization, after seed shows money or valuables one carries unintentionally, adopt Nursery processing is carried out with water planting, the nursery stage, the EC value for controlling nutrient solution was 1.0ms/cm per each 2 days pouring one time of nutrition liquid, PH is 6.0-7.0;
(2) culturing and planting
When red Luo Sha romaine lettuce seedling length to 4-5 piece true leaf, the red Luo Sha romaine lettuce seedling replanting of neat and consistent is selected to calmly It plants on plate, field planting line space is 15cm × 20cm, the red Luo Sha romaine lettuce after field planting is put into nutrition liquid bath and is cultivated, nutrition The EC value of liquid is 1.8ms/cm, and PH 6.0-7.0, solution temperature is 20-22 DEG C, dissolved oxygen amount 5-6mg/L.Environment temperature item Part are as follows: day temperature maintains 20-23 DEG C, and night temperatures maintain 18-20 DEG C, relative air humidity 60-70%, with vegetable The extension in dish growth period, during entire culturing and planting is gradually increased in nutrient solution EC value, illumination condition uses the full spectrum lamp of LED, Intensity of illumination is 250 μm of ol/m2S, periodicity of illumination 8h;
(3) it harvests
By the red Luo Sha romaine lettuce after field planting 20d, using the full spectrum intermittent illumination of LED and ultra-blue-light 450nm intermittent illumination In conjunction with mode, and the full spectrum intermittent illumination of LED first irradiates, and ultra-blue-light 450nm intermittent illumination irradiates again again, non-crossing photograph It penetrates, vegetables is coloured, test group processing scheme is as follows:
The full spectrum of LED: duty ratio 90%, the illumination duration is 18s in the periodicity of illumination of gap, periodicity of illumination 5 hours, light According to 250 μm of ol/ ㎡ s of intensity;
Ultra-blue-light: duty ratio 50%, the illumination duration is 1s in the periodicity of illumination of gap, periodicity of illumination 10 hours, light According to 250 μm of ol/ ㎡ s of intensity;
2d is handled, is harvested.
11 processing scheme of control group is as follows:
The full spectrum of LED: continuous illumination mode, 250 μm of ol/m of intensity of illumination2S, photoperiod 9.5h handle 2d, carry out Harvesting.
12 processing scheme of control group is as follows:
Ultra-blue-light: continuous illumination mode, 250 μm of ol/m of intensity of illumination2S, photoperiod 9.5h handle 2d, carry out Harvesting.
(4) result and analysis
After harvesting, 50 plants of red Luo Sha romaine lettuce are randomly selected, to its ground fresh weight, anthocyanidin content and Vitamin C content It measures, is averaged, result is as shown in the table:
As a result: embodiment 5 at least improves 15% than 12 anthocyanidin content of control group, and ground fresh weight is improved than control group 11 1.4%;
Embodiment 6
The present embodiment 5 uses purple U.S. romaine lettuce as sample, and specific incubation step is as follows:
(1) seeding and seedling raising
Purple U.S. romaine lettuce seed is chosen, after purple U.S. romaine lettuce seed is carried out clear water seed soaking, is multicast in sponge square, every cave one Grain;Then there is the sponge block of purple U.S. romaine lettuce seed to be placed in germinating box sowing again and carry out vernalization, after seed shows money or valuables one carries unintentionally, using water Training carries out nursery processing, and the nursery stage, the EC value for controlling nutrient solution was 0.8ms/cm, and pH is per each 2 days pouring one time of nutrition liquid 6.0-7.0;
(2) culturing and planting
When purple U.S. romaine lettuce length to 4-5 piece true leaf, select on the purple U.S. romaine lettuce seedling replanting to field planting plate of neat and consistent, it is fixed Plant line space is 15cm × 20cm, and the U.S. vegetables of purple after field planting are put into nutrition liquid bath and are cultivated, are planted using nutrient film Training technology, the EC value of nutrient solution are 1.5ms/cm, and PH 6.0-7.0, solution temperature is 20-22 DEG C, dissolved oxygen amount 5-6mg/ L.Ambient temperature conditions are as follows: day temperature maintains 20-23 DEG C, and night temperatures maintain 18-20 DEG C, and relative air humidity is 60-70%, with the extension of vegetable growth phase, during entire culturing and planting is gradually increased in nutrient solution EC value, illumination condition is adopted With the full spectrum lamp of LED, intensity of illumination is 250 μm of ol/m2S, periodicity of illumination 10h;
(3) it harvests
By the U.S. romaine lettuce of purple after field planting 24d, using the normal continuous illumination of the full spectrum of LED and ultra-blue-light 450nm intermittent light According to the mode of combination, vegetables are coloured, test group processing scheme is as follows:
The full spectrum of LED: continuous illumination, periodicity of illumination 8 hours, 200 μm of ol/ ㎡ s of intensity of illumination;
Ultra-blue-light: duty ratio 75%, the illumination duration is 3s in the periodicity of illumination of gap, periodicity of illumination 8 hours, illumination 200 μm of ol/ ㎡ s of intensity;
3d is handled, is harvested.
13 processing scheme of control group is as follows:
The full spectrum of LED: continuous illumination, periodicity of illumination 8 hours, 200 μm of ol/ ㎡ s of intensity of illumination;
Ultra-blue-light: continuous illumination, 200 μm of ol/m of intensity of illumination2S, photoperiod 6h handle 3d, are harvested.
(4) result and analysis
After harvesting, 50 plants of purple U.S. romaine lettuce are randomly selected, to its ground fresh weight, anthocyanidin content, Vitamin C content and can Dissolubility albumen measures, and is averaged, and result is as shown in the table:
As a result: embodiment 6 at least improves 19% than 13 anthocyanidin content of control group, and ground fresh weight is improved than control group 13 7.3%;
(4) different luminous environment regulations are respectively adopted to handle the aubergine vegetables of Later growth, by form number Accordingly and index of correlation acquisition measures, and data result is apparent that in analytical table, regulates and controls skill using luminous environment of the invention Art scheme, the ground fresh weight of aubergine vegetables have a more apparent raising, while anthocyanidin content, Vitamin C content and solvable Property protein content is also significantly increased, and the aubergine vegetables under luminous environment control technique of the present invention can preferably realize coloring, It is avoided that the problem of underproduction occur again simultaneously.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or the terminal device that include a series of elements not only include those Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or end The intrinsic element of end equipment.In the absence of more restrictions, being limited by sentence " including ... " or " including ... " Element, it is not excluded that there is also other elements in process, method, article or the terminal device for including the element.This Outside, herein, " being greater than ", " being less than ", " being more than " etc. are interpreted as not including this number;" more than ", " following ", " within " etc. understand Being includes this number.
Although the various embodiments described above are described, once a person skilled in the art knows basic wounds The property made concept, then additional changes and modifications can be made to these embodiments, so the above description is only an embodiment of the present invention, It is not intended to limit scope of patent protection of the invention, it is all to utilize equivalent structure or equivalent made by present specification Process transformation, being applied directly or indirectly in other relevant technical fields, similarly includes in patent protection model of the invention Within enclosing.

Claims (8)

1. a kind of luminous environment of regulation plant metabolites regulates and controls method, which is characterized in that be added in the plant culture stage A kind of few intermittent illumination, promotes the accumulation of secondary metabolites in plant.
2. the method according to claim 1, wherein the intermittent light is the interval of two or more light quality Light, intermittent light radiation modality are cross-fire or non-crossing irradiation.
3. according to the method described in claim 2, it is characterized in that, in cross-fire mode, the duty ratio < of each intermittent light 50%.
4. according to the method described in claim 2, it is characterized in that, in non-crossing radiation modality, the duty ratio of each intermittent light >= 50%.
5. the method according to claim 1, wherein intermittent illumination light quality is monochromatic spectrum or Quan Guangpu, one The illumination duration is 1s-20s in a gap periodicity of illumination, and periodicity of illumination is≤for 24 hours/d.
6. according to the method described in claim 5, it is characterized in that, the intermittent illumination is ultra-blue-light 450nm intermittent illumination.
7. the method according to claim 1, wherein intermittent illumination light intensity is 200-300 μm of ol/ ㎡ s.
8. a kind of planting cultural method, which is characterized in that specific step is as follows:
(1) seeding and seedling raising
After aubergine vegetable seeds is carried out clear water seed soaking, it is multicast in sponge square, 1, every cave;Place it in germinating box into Row vernalization carries out nursery processing, the nursery stage was per each 2 days pouring one time of nutrition liquid after seed shows money or valuables one carries unintentionally;
(2) culturing and planting
When vegetable seedling length to 4-5 piece true leaf, culturing and planting, environmental condition are carried out under illumination condition are as follows: diurnal temperature dimension It holds at 20-23 DEG C/18-20 DEG C, relative air humidity 60-70%, using continuous illumination, 200-300 μm of ol/ of intensity of illumination ㎡ s, periodicity of illumination 8-12h/d;
(3) it harvests
Within the last week picking time, vegetables are coloured using luminous environment as claimed in claim 1 to 7 regulation method, are located Manage vegetables 2-7d.
CN201910909834.7A 2019-09-25 2019-09-25 A kind of luminous environment regulation method of regulation plant metabolism substance Pending CN110521566A (en)

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CN112056169A (en) * 2020-07-24 2020-12-11 福建省中科生物股份有限公司 Method for prolonging florescence of non-bulbous herbaceous flowers in plant factory
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