CN108541574A - A kind of cultural method improving Lycopene in Tomatoes content - Google Patents
A kind of cultural method improving Lycopene in Tomatoes content Download PDFInfo
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- CN108541574A CN108541574A CN201810417128.6A CN201810417128A CN108541574A CN 108541574 A CN108541574 A CN 108541574A CN 201810417128 A CN201810417128 A CN 201810417128A CN 108541574 A CN108541574 A CN 108541574A
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- tomato
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
Abstract
The invention discloses a kind of cultural methods improving Lycopene in Tomatoes content, include the following steps：Tissue-cultured Tomato Plants are placed in fluid nutrient medium and are cultivated, when tomato forms bud, methyl jasmonate and mannitol are added in the fluid nutrient medium, make the final concentration of 2.5 ~ 4.7mg/L of methyl jasmonate in liquid medium, final concentration of 0.3 ~ 1.0g/L of mannitol, continue culture to tomato as a result, maturation.During tomato cultivation, the tomato cellulose content in harvest tomato can be improved using methyl jasmonate and mannitol, improves Tomato Quality.
Description
Technical field
The present invention relates to planting technical fields, and in particular to a kind of cultivation side for improving Lycopene in Tomatoes content
Method.
Background technology
Lycopene is a kind of natural pigment contained in plant.It is primarily present in the ripening fruits of plant of Solanaceae tomato
In.It is most one of the powerful antioxidant being found in the plant of nature at present.Lycopene removes the effect of free radical
Other carotenoid and vitamin E are outclassed, singlet-oxygen quenching rate constant is 100 times of vitamin E.It can have
The prevention of effect is because of aging, various diseases caused by immunity degradation, therefore is concerned.
Lycopene is an important indicator for weighing Tomato Quality quality.Tomato greenhouse plantation at present is to improve tomato production
The important measures of amount.However since a large amount of put into that greenhouse vegetable production relies primarily on rich water improves yield, water is not only caused
The waste of resource and fertilizer also results in vegetables incidence height, quality decline.Meanwhile Shaoshi or not organic fertilizer, inclined nitrogen fertilizer application,
Heavy dressing phosphate fertilizer, Shaoshi or not potassium application the phenomenon that it is also commonplace, this long-term high nitrogen, rich phosphorus, few potassium nutrition condition, cause
Nutrient supply is lacked of proper care, it has also become limits one of the principal element that current greenhouse tomato quality improves.
It focuses mostly in the reasonable supply of N P and K and moisture for the method for improving Lycopene in Tomatoes content at present,
But a unified conclusion is not obtained, this may complicated reciprocation be related between Water and fertilizer factors, and it is still necessary into one
Step further investigation.
Invention content
In view of this, the present invention provides a kind of cultural methods improving Lycopene in Tomatoes content, using to tomato
Tissue-cultured seedling is induced, and the lycopene content in tomato is improved, and promotes Tomato Quality.
Technical scheme is as follows：
The present invention provides a kind of cultural methods improving Lycopene in Tomatoes content, include the following steps：By tomato tissue culture
Seedling is placed in fluid nutrient medium and is cultivated, and when tomato forms bud, methyl jasmonate is added in the fluid nutrient medium
And mannitol, make the final concentration of 2.5 ~ 4.7mg/L of methyl jasmonate in liquid medium, mannitol final concentration of 0.3 ~
1.0g/L continues culture to tomato as a result, maturation.
Preferably, the fluid nutrient medium is the MS fluid nutrient mediums of improvement, and the fluid nutrient medium of the improvement is in MS
Sucrose is added in fluid nutrient medium, makes its final concentration of 30 ~ 40g/L.
It is further preferred that the pH value of the fluid nutrient medium is 6.0 ~ 7.5.
Preferably, the incubation of the Tissue-cultured Tomato Plants includes：Tomato explant is cultivated in inducing culture, is obtained
To callus；The callus cultivates differentiation in MS minimal mediums and obtains adventitious bud；The adventitious bud is transferred to life again
It is cultivated in root culture medium, Tissue-cultured Tomato Plants is obtained after taking root.
Further, the inducing culture is：Based on MS minimal mediums, also contain 6- benzyl aminoadenines
1.5 ~ 2.5mg/L, polyvinylpyrrolidone 8 ~ 15 mg/L, 5 ~ 8g/L of agar, 30 ~ 40g/L of sucrose.
Further, the root media is：Based on MS minimal mediums, further include indolebutyric acid 0.8 ~
1.2mg/L, 5 ~ 10g/L of agar, 30 ~ 40g/L of sucrose.
Preferably, the length of the Tissue-cultured Tomato Plants is not less than 10cm.
Preferably, the temperature of the Tissue-cultured Tomato Plants culture is 23 ~ 30 DEG C.
Preferably, the photoperiod of the Tissue-cultured Tomato Plants culture is 12 ~ 18 hours, and the dark period is 12 ~ 6 hours.
Preferably, when the Tissue-cultured Tomato Plants culture, air is passed through in the Liquid Culture.
Compared with the existing technology, the present invention has the following advantages：
The present invention provides a kind of cultural methods improving Lycopene in Tomatoes content, include the following steps：By tomato tissue culture
Seedling is placed in fluid nutrient medium and is cultivated, and when tomato forms bud, methyl jasmonate is added in the fluid nutrient medium
And mannitol, make the final concentration of 2.5 ~ 4.7mg/L of methyl jasmonate in liquid medium, mannitol final concentration of 0.3 ~
1.0g/L continues culture to tomato as a result, maturation.The present invention, to when forming bud, adds in liquid medium in tomato growth
Enter methyl jasmonate and mannitol, induction tomato yields positive results, and promotes Fruit Ripening of Tomato, improves the tomato in harvest time tomato
Lycopene content.Methyl jasmonate, which plays, adjusts tomato growth metabolism, enhances photosynthesis, improves suction of the tomato plant to water, fertilizer
It receives, adjusts the effect of plant internal water balance, excite the expression of lycopene in tomato dna.Mannitol being capable of Cooperative Excitation
Methyl jasmonate further promotes the expression of Lycopene in Tomatoes to the adjustment effect of tomato, improves Lycopene in Tomatoes
Content.
Specific implementation mode
The present invention provides a kind of cultural methods improving Lycopene in Tomatoes content, include the following steps：By tomato
Tissue-cultured seedling is placed in fluid nutrient medium and is cultivated, and when tomato forms bud, jasmonic is added in the fluid nutrient medium
Methyl esters and mannitol, make the final concentration of 2.5 ~ 4.7mg/L of methyl jasmonate in liquid medium, mannitol it is final concentration of
0.3 ~ 1.0g/L continues culture to tomato as a result, maturation.
Present invention preferably employs Tissue-cultured Tomato Plants to be cultivated.Tissue-cultured seedling, which has been sloughed, to be carried in tomato seeds or explant
Virus, and well developed root system are conducive to the absorption of nutrient, improve the growth rate of tomato.The present invention does not have the variety type of tomato
There is particular determination, using any tomato variety present in this field.
The cultivating process of Tissue-cultured Tomato Plants of the present invention preferably includes：Tomato explant is cultivated in inducing culture, is obtained
To callus；The callus cultivates differentiation in MS minimal mediums and obtains adventitious bud；The adventitious bud is transferred to life again
It is cultivated in root culture medium, Tissue-cultured Tomato Plants is obtained after taking root.
The present invention is not particularly limited the type of tomato explant, can be tomato seeds, tomato plant arbitrary portion
Tissue or organ.After tomato explant is sterilized, it is placed in inducing culture and is cultivated, obtain callus.The present invention
The mode of tomato explant disinfection is not particularly limited, using the routine disinfection mode in this field, such as mercuric chloride, alcohol
Or hypochlorous acid disinfection.Inducing culture of the present invention is preferably：Based on MS minimal mediums, also contain 6- benzyl amino glands
1.5 ~ 2.5mg/L of purine, polyvinylpyrrolidone 8 ~ 15 mg/L, 5 ~ 8g/L of agar, 30 ~ 40g/L of sucrose, more preferably MS bases
Contain 6- benzyl aminoadenine 2.0mg/L, polyvinylpyrrolidone 10 mg/L, agar 7g/L, sucrose 35g/L in basal culture medium.
Callus is obtained after 20 ~ 30 days.
Callus is cultivated differentiation in MS minimal mediums and obtains adventitious bud or the young stem of differentiation by the present invention.
Adventitious bud is transferred to culture in root media and taken root by the present invention, obtains Tissue-cultured Tomato Plants.It is of the present invention to take root
Culture medium is preferably：Further include 0.8 ~ 1.2mg/L of indolebutyric acid, 5 ~ 10g/L of agar, sucrose based on MS minimal mediums
30 ~ 40g/L contains indolebutyric acid 1.0mg/L, agar 7g/L, sucrose 35g/L further preferably in MS minimal mediums.
In the tomato tissue incubation of the present invention, there is no special restriction to the preparation method of culture medium, using ability
Culture medium preparation method known to field technique personnel is prepared.The incubation of Tissue-cultured Tomato Plants of the present invention is preferably in group
It knits in culturing room and carries out, the temperature of the tissue culture room is preferably 23 ~ 30 DEG C, more preferably 24 ~ 26 DEG C；The tissue cultures
The humidity of room is preferably 40 ~ 60%；Intensity of illumination is preferably the Lx of 1500 Lx ~ 3 000, more preferably the Lx of 2000 Lx ~ 2500；
Light application time is daily 12 ~ 18 h, more preferably 13 ~ 16 h.
Tissue-cultured Tomato Plants are placed directly in fluid nutrient medium and cultivate by the present invention, can effectively keep the one of culture environment
Cause property, avoids the hardening process before nursery planting, is conducive to the fast-growth of tomato, improves survival rate.Preferred kind of the present invention
The root long of eggplant tissue-cultured seedling is not shorter than 10cm, more preferably 15 ~ 25cm.
The fluid nutrient medium of the present invention is preferably the MS fluid nutrient mediums improved, and the fluid nutrient medium of the improvement is in MS
Sucrose is added in fluid nutrient medium, makes its final concentration of 30 ~ 40g/L, more preferably 32 ~ 37g/L.The addition of sucrose is conducive to
The precursor activated substance of tomato cylinder accumulation lycopene provides material base for the conversion of lycopene.Liquid of the present invention
The pH value of body culture medium is preferably 6.0 ~ 7.5, and more preferably 6.5 ~ 7.0.
Methyl jasmonate and mannitol is added when tomato forms bud in the present invention in the fluid nutrient medium.Jasmine
Sour methyl esters plays the role of adjusting tomato growth metabolism, and tomato is promoted to yield positive results, and lycopene contains when improving Tomato Ripening
Amount.Mannitol Cooperative Excitation methyl jasmonate improves the growth quality of tomato to the adjusting facilitation of tomato.Jasmine in the present invention
The final concentration of jasmine acid methyl esters in liquid medium is preferably 3.0 ~ 4.2mg/L, and the final concentration of mannitol is preferably 0.5 ~
0.8mg/L。
The temperature that Tissue-cultured Tomato Plants of the present invention are cultivated in liquid medium is preferably 23 ~ 30 DEG C, more preferably 25 ~
27 DEG C, the photoperiod is preferably 12 ~ 18 hours/day, more preferably 14 ~ 16 hours/day.In order to ensure tomato in liquid medium
Growth efficiency, the present invention is preferably passed through air, improves the oxygen content of tomato root in liquid medium, increases root system pair
Nutrient absorption in fluid nutrient medium improves growth efficiency.When tomato growth to result maturation, the tomato red in tomato is measured
Cellulose content.
The present invention preferably measures the content of cultural method Lycopene in Tomatoes of the present invention with spectrophotometry.As a result table
Bright, method of the invention can significantly improve the lycopene content of tomato, greatly improve the growth quality of cultivated tomato.
Below in conjunction with the embodiment in the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1：
Cultivate Tissue-cultured Tomato Plants
Using the blade of red No. 1 tomato in capital as explant, then cleaning down under tap water is placed in 5% Tween-20 and impregnates
It 5 minutes, is rinsed 3-4 times with sterile purified water, 0.1% mercuric chloride (HgCl2) surface sterilizing 4 minutes, it is rinsed with sterile purified water
4-5 times.
It is that 1cm × 0.5cm or so is used as explant that leaf tissue, which is cut into size, is inoculated into inducing culture, described
Inducing culture is：12 mg/L+ agar of MS minimal medium+6- benzyl aminoadenine 2.4mg/L+ polyvinylpyrrolidones
5g/L+ sucrose 30g/L.
Callus is grown after 25 days, callus is gone into MS minimal mediums, is cultivated 7 days, callus is divided into
Adventitious bud.
Adventitious bud is inoculated into be transferred in root media and is cultivated, the root media is：MS minimal mediums+indoles
Butyric acid 0.8mg/L+ agar 5g/L+ sucrose 30g/L.
It is 25 DEG C that tissue culture room cultivation temperature, which is arranged, humidity 50%, intensity of illumination 2000Lx, 16 hours light weeks
Phase, 8 hours dark periods.
Embodiment 2
When the Tissue-cultured Tomato Plants root long of example 1 to be performed is more than 10cm, Tissue-cultured Tomato Plants are placed in the cultures of the MS containing 40g/L sucrose
It is cultivated in base.It is 25 DEG C to control cultivation temperature, humidity 60%, photoperiod 12h, and is passed through sky in liquid medium
Gas.
When tomato forms bud, methyl jasmonate and mannitol are added in liquid medium, methyl jasmonate is made to exist
Final concentration of 3.0mg/L in fluid nutrient medium, the final concentration of 1.0g/L of mannitol, continues to cultivate.Tomato blooms after 18 days,
36 days fruit annesls, 55 days fruit maturations.
Embodiment 3
When the Tissue-cultured Tomato Plants root long of example 1 to be performed is more than 10cm, Tissue-cultured Tomato Plants are placed in the cultures of the MS containing 30g/L sucrose
It is cultivated in base.It is 28 DEG C to control cultivation temperature, humidity 40%, photoperiod 14h, and is passed through sky in liquid medium
Gas.
When tomato forms bud, methyl jasmonate and mannitol are added in liquid medium, methyl jasmonate is made to exist
Final concentration of 4.0mg/L in fluid nutrient medium, the final concentration of 0.3g/L of mannitol, continues to cultivate.Tomato blooms after 17 days,
37 days fruit annesls, 53 days fruit maturations.
Embodiment 4
When the Tissue-cultured Tomato Plants root long of example 1 to be performed is more than 10cm, Tissue-cultured Tomato Plants are placed in the cultures of the MS containing 35g/L sucrose
It is cultivated in base.It is 26 DEG C to control cultivation temperature, humidity 50%, photoperiod 16h, and is passed through sky in liquid medium
Gas.
When tomato forms bud, methyl jasmonate and mannitol are added in liquid medium, methyl jasmonate is made to exist
Final concentration of 4.5mg/L in fluid nutrient medium, the final concentration of 0.8g/L of mannitol, continues to cultivate.Tomato blooms after 18 days,
35 days fruit annesls, 51 days fruit maturations.
Comparative example 1
When the Tissue-cultured Tomato Plants root long of example 1 to be performed is more than 10cm, Tissue-cultured Tomato Plants are placed in the cultures of the MS containing 35g/L sucrose
It is cultivated in base.It is 26 DEG C to control cultivation temperature, humidity 50%, photoperiod 16h, and is passed through sky in liquid medium
Gas.Until Fruit Ripening of Tomato.Tomato blooms for 20 days after forming bud, 40 days fruit annesls, 60 days fruit maturations.
Comparative example 2
When the Tissue-cultured Tomato Plants root long of example 1 to be performed is more than 10cm, Tissue-cultured Tomato Plants are placed in the cultures of the MS containing 35g/L sucrose
It is cultivated in base.It is 26 DEG C to control cultivation temperature, humidity 50%, photoperiod 16h, and is passed through sky in liquid medium
Gas.
When tomato forms bud, methyl jasmonate is added in liquid medium, makes methyl jasmonate in Liquid Culture
Final concentration of 4.5mg/L in base, continues to cultivate.Tomato blooms after 19 days, 38 days fruit annesls, 57 days fruit maturations.
Comparative example 3
When the Tissue-cultured Tomato Plants root long of example 1 to be performed is more than 10cm, Tissue-cultured Tomato Plants are placed in the cultures of the MS containing 35g/L sucrose
It is cultivated in base.It is 26 DEG C to control cultivation temperature, humidity 50%, photoperiod 16h, and is passed through sky in liquid medium
Gas.
When tomato forms bud, mannitol is added in liquid medium, makes the end of mannitol in liquid medium
A concentration of 0.8g/L continues to cultivate.Tomato blooms after 20 days, 40 days fruit annesls, 60 days fruit maturations.
By embodiment 4 and comparative example 1 ~ 3, illustrates that methyl jasmonate can adjust the growth rate of tomato, shorten tomato
Yield positive results the time, accelerate Tomato Ripening, and have certain relationship with methyl jasmonate concentration；Mannitol being capable of synergistic activation jasmine
Jasmine acid methyl esters improves tomato growth quality to the growth regulating ability of tomato.
Embodiment 5
Detect lycopene content in the mature tomato of embodiment 4 and comparative example 1 ~ 3
After fresh tomato is cleaned, stripping and slicing smashs to pieces to obtain tomato puree.The iodine flask of 3 150 mL is taken, it is each that 20 g tomato purees are added
With 20 mL sesame oil, seals, is protected from light, extract 4h at room temperature, obtain extract liquor.2 mL of extract liquor is taken, is settled to anhydrous ether
50 mL make reference with sesame oil, and absorbance is measured at 502nm.As a result it see the table below：
Absorbance value of the extract liquor that 1 different disposal tomato of table is extracted at 502nm
| Embodiment | Processing | Absorbance |
| Embodiment 4 | Methyl jasmonate+mannitol | 0.206** |
| Comparative example 1 | Conventional liq culture | 0.131 |
| Comparative example 2 | Methyl jasmonate | 0.174* |
| Comparative example 3 | Mannitol | 0.132 |
It is big according to the absorbance in tomato extract liquor in the present invention due to the content proportional of absorbance and lycopene
How much is the content of small determining lycopene.As can be seen from Table 1, treatment with mannitol is added in liquid medium and is not added with sweet dew
Alcohol influences less the lycopene content in harvest tomato, and the two is not significantly different.Add in the fluid nutrient medium of tomato
Enter methyl jasmonate, the content of Lycopene in Tomatoes can be significantly improved.With the liquid containing methyl jasmonate and mannitol
After culture medium cultivated tomato, obtained tomato red lycopene content highest is significantly higher than independent methyl jasmonate treatment and obtains
Lycopene in Tomatoes content.Illustrate that methyl jasmonate and mannitol can improve the tomato cellulose content in harvest tomato, carries
High Tomato Quality.
It can be that professional and technical personnel in the field realize or use that the above embodiment, which is intended to illustrate the present invention, to above-mentioned implementation
Mode, which is modified, will be apparent to those skilled in the art, therefore the present invention is including but not limited to above-mentioned
Embodiment, it is any to meet the claims or specification description, meet and principles disclosed herein and novelty, creation
The method of property feature, technique, product, each fall within protection scope of the present invention.
Claims (10)
1. a kind of cultural method improving Lycopene in Tomatoes content, which is characterized in that include the following steps：By tomato tissue culture
Seedling is placed in fluid nutrient medium and is cultivated, and when tomato forms bud, methyl jasmonate is added in the fluid nutrient medium
And mannitol, make the final concentration of 2.5 ~ 4.7mg/L of methyl jasmonate in liquid medium, mannitol final concentration of 0.3 ~
1.0g/L continues culture to tomato as a result, maturation.
2. cultural method according to claim 1, which is characterized in that the fluid nutrient medium is the MS Liquid Cultures of improvement
Base, the fluid nutrient medium of the improvement are that sucrose is added in MS fluid nutrient mediums, make its final concentration of 30 ~ 40g/L.
3. cultural method according to claim 1 or 2, which is characterized in that the pH value of the fluid nutrient medium be 6.0 ~
7.5。
4. cultural method according to claim 1, which is characterized in that the cultivating process of the Tissue-cultured Tomato Plants includes：It will
Tomato explant is cultivated in inducing culture, obtains callus；The callus is cultivated in MS minimal mediums to be divided
Change obtains adventitious bud；The adventitious bud is transferred in root media again to be cultivated, and Tissue-cultured Tomato Plants are obtained after taking root.
5. cultural method according to claim 5, which is characterized in that the inducing culture is：With MS minimal mediums
Based on, also contain 6- benzyl 1.5 ~ 2.5mg/L of aminoadenine, polyvinylpyrrolidone 8 ~ 15 mg/L, 5 ~ 8g/L of agar, sugarcane
30 ~ 40g/L of sugar.
6. cultural method according to claim 5, which is characterized in that the root media is：With MS minimal mediums
Based on, further include 0.8 ~ 1.2mg/L of indolebutyric acid, 5 ~ 10g/L of agar, 30 ~ 40g/L of sucrose.
7. cultural method according to claim 1, which is characterized in that the length of the Tissue-cultured Tomato Plants is not less than 10cm.
8. cultural method according to claim 1, which is characterized in that the Tissue-cultured Tomato Plants are cultivated in liquid medium
Temperature be 23 ~ 30 DEG C.
9. cultural method according to claim 1, which is characterized in that the Tissue-cultured Tomato Plants are cultivated in liquid medium
Photoperiod be 12 ~ 18 hours/day, the dark period be 12 ~ 6 hours/day.
10. cultural method according to claim 1, which is characterized in that the Tissue-cultured Tomato Plants are trained in liquid medium
When supporting, air is passed through in the Liquid Culture.
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| CN111296263A (en) * | 2020-03-24 | 2020-06-19 | 陕西科技大学 | Hydroponic method for relieving toxic effect of cadmium on tomatoes by utilizing methyl jasmonate and application of methyl jasmonate |
| CN111802389A (en) * | 2020-08-07 | 2020-10-23 | 中国农业科学院茶叶研究所 | Exogenous induction composition, exogenous inducer and method for improving carotenoid in tea |
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| CN111802389B (en) * | 2020-08-07 | 2021-12-28 | 中国农业科学院茶叶研究所 | Exogenous induction composition, exogenous inducer and method for improving carotenoid in tea |
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Application publication date: 20180918 |