CN109832269B - Seed treatment agent for promoting germination of corn seeds and preparation method and application thereof - Google Patents

Seed treatment agent for promoting germination of corn seeds and preparation method and application thereof Download PDF

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CN109832269B
CN109832269B CN201910294136.0A CN201910294136A CN109832269B CN 109832269 B CN109832269 B CN 109832269B CN 201910294136 A CN201910294136 A CN 201910294136A CN 109832269 B CN109832269 B CN 109832269B
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徐建明
季文漪
李师默
刘庭武
杨文杰
李正鹏
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Huaiyin Normal University
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Abstract

The invention provides a seed treatment agent for promoting corn seed germination, and belongs to the technical field of seed treatment. The seed treating agent takes water as a dispersing agent and comprises the following dispersoids in parts by mass: 15-25 parts of catechol, 1215-25 parts of vitamin B, 15000-30000 parts of zinc sulfate and 15000-25000 parts of manganese sulfate. The seed treatment agent provided by the invention is diluted by 100 times of water to carry out corn seed soaking treatment for more than 12 hours, so that the germination potential of seeds can be improved, the germination potential is increased by 58.42% on average and the germination rate is increased by 33.16% on average; the fresh weight of the overground part of the corn seedling is averagely increased by 26.78 percent compared with the control, and the dry weight is averagely increased by 22.95 percent compared with the control; the fresh weight of the root system is increased by 65.10 percent on average compared with the control, and the dry weight is increased by 34.22 percent on average compared with the control.

Description

Seed treatment agent for promoting germination of corn seeds and preparation method and application thereof
Technical Field
The invention relates to the technical field of seed treatment, in particular to a seed treatment agent for promoting corn seed germination and a preparation method and application thereof.
Background
Corn is one of the main food crops in China, and the planting area and the total yield of the corn are second to those of rice and wheat. Thus, the level of corn production plays an important role in agricultural production. In corn cultivation, high and stable yield of corn plays an important role in high-efficiency cultivation technology besides high-quality corn varieties. In the cultivation technology, high-quality seeds are a key factor for increasing yield, so that the treatment of corn seeds is particularly important.
Corn seed soaking treatment is one of the common measures in corn cultivation, and in recent years, corn seed soaking agents are rapidly developed, and mainly focus on research and application in the aspects of preventing and controlling soil insects of corn and regulating seed vigor. The seed treatment for underground corn pests is more, and most of seed soaking agents for regulating and controlling seed vitality are single-element seed soaking agents. Therefore, the research on the synergistic formation of the novel corn seed treatment agent by the multiple substances can improve the activity of the corn seeds, accelerate the germination of the seeds and the growth of seedlings and improve the stress resistance of the corn, thereby being beneficial to laying a foundation for the high yield and stable yield of the corn.
Disclosure of Invention
In view of the problems in the background art, the invention aims to provide a seed treatment agent for promoting germination of corn seeds, a preparation method and an application thereof, which can improve the vitality of the corn seeds, accelerate the germination of the seeds and the growth of seedlings, enhance the stress resistance of the corn and lay a foundation for high yield and stable yield of the corn.
The invention provides the following technical scheme:
a seed treatment agent for promoting germination of corn seeds takes water as a dispersing agent and comprises the following dispersoids in parts by mass: 15-25 parts of catechol, 1215-25 parts of vitamin B, 15000-30000 parts of zinc sulfate and 15000-25000 parts of manganese sulfate.
Preferably, the seed treatment agent takes water as a dispersing agent and comprises the following dispersoids in parts by mass: 18-22 parts of catechol, 1218-22 parts of vitamin B, 18000-22000 parts of zinc sulfate and 18000-22000 parts of manganese sulfate.
Preferably, the seed treatment agent takes water as a dispersing agent and comprises the following dispersoids in parts by mass: 20 parts of catechol, 1220 parts of vitamin B, 20000 parts of zinc sulfate and 20000 parts of manganese sulfate.
Preferably, the seed treatment agent takes water as a dispersion system, and comprises dispersoids with the following concentrations: 15-20 mg/L of catechol, 1215-20 mg/L of vitamin B, 20-30 g/L of zinc sulfate and 15-20 g/L of manganese sulfate.
Preferably, the seed treatment agent takes water as a dispersion system, and comprises dispersoids with the following concentrations: 20mg/L of catechol, 1220 mg/L of vitamin B, 20g/L of zinc sulfate and 20g/L of manganese sulfate.
The invention provides a preparation method of the seed treatment agent, which comprises the following steps:
(1) mixing zinc sulfate with water to obtain a first solution;
(2) mixing vitamin B12 with the first solution, and dissolving for more than 1.5h at 50-70 ℃ to obtain a second solution;
(3) mixing manganese sulfate with the second solution to obtain a third solution;
(4) and mixing the catechol with the third solution to obtain the seed treating agent.
Preferably, the dissolving time of the step (2) is more than or equal to 2 hours.
The invention also provides an application of the seed treating agent or the seed treating agent prepared by the preparation method in promoting corn seed germination.
Preferably, the application comprises seed soaking, wherein seed soaking liquid is water diluted liquid of the seed treating agent or the seed treating agent prepared by the preparation method; the dilution times of the water diluent are 50-200 times; the seed soaking time is 8-24 h.
Preferably, the seed soaking time is 12-16 h.
The invention provides a seed treatment agent for promoting germination of corn seeds, which takes water as a dispersing agent and comprises dispersoids in the following mass part ratio: 15-25 parts of catechol, 1215-25 parts of vitamin B, 15000-30000 parts of zinc sulfate and 15000-25000 parts of manganese sulfate. The seed treatment agent provided by the invention comprises catechol, vitamin B12, zinc sulfate and manganese sulfate, and all the components have synergistic effect, not only have regulation and control functions, but also have special nutrition functions, and are different from the conventional single insecticidal, bactericidal, regulation and control and nutrient agent sold in the market. The seed treatment agent provided by the invention is diluted by 100 times of water to carry out corn seed soaking treatment for more than 12 hours, so that the germination potential of seeds can be improved, the germination potential is increased by 58.42% on average and the germination rate is increased by 33.16% on average; the fresh weight of the overground part of the corn seedling is averagely increased by 26.78 percent compared with the control, and the dry weight is averagely increased by 22.95 percent compared with the control; the fresh weight of the root system is increased by 65.10 percent on average compared with the control, and the dry weight is increased by 34.22 percent on average compared with the control.
Detailed Description
The invention provides a seed treatment agent for promoting germination of corn seeds, which takes water as a dispersing agent and comprises the following dispersoids in parts by mass: 15-25 parts of catechol, 1215-25 parts of vitamin B, 15000-30000 parts of zinc sulfate and 15000-25000 parts of manganese sulfate.
The seed treatment agent provided by the invention comprises pyrocatechol, vitamin B12, zinc sulfate and manganese sulfate. In the invention, the dosage of the catechol is 15-25 parts, preferably 18-22 parts, and more preferably 20 parts; the dosage of the vitamin B12 is 15-25 parts, preferably 18-22 parts, and more preferably 20 parts; the using amount of the zinc sulfate is 15000-30000 parts, preferably 18000-25000 parts, and more preferably 20000 parts; the using amount of the manganese sulfate is 15000-30000 parts, preferably 18000-25000 parts, and more preferably 20000 parts.
The seed treatment agent of the present invention uses water as a dispersion, and the concentration of each dispersoid is preferably: 15-20 mg/L of catechol, 1215-20 mg/L of vitamin B, 20-30 g/L of zinc sulfate and 15-20 g/L of manganese sulfate. In the invention, the concentration of the catechol is preferably 15-20 mg/L, and more preferably 20 mg/L; the concentration of the vitamin B12 is preferably 15-20 mg/L, and more preferably 20 mg/L; the concentration of the zinc sulfate is preferably 20-30 g/L, and more preferably 20 g/L; the concentration of the manganese sulfate is preferably 15-20 g/L, and more preferably 20 g/L.
The seed treatment agent provided by the invention takes water as a dispersing agent and is prepared by matching components including pyrocatechol, vitamin B12, zinc sulfate and manganese sulfate. Catechol and vitamin B12 belong to growth conditioners, have the functions of promoting seed germination and seedling growth, but have limited effect; zinc sulfate and manganese sulfate belong to mineral elements, have certain promotion effect on germination of corn seeds, and have limited effect. The four components are combined and are mutually cooperated, so that the physiological basis in the seeds is changed in the seed germination process, the germination of the seeds and the growth and development of the seedlings after the seeds germinate are facilitated, the mobility of the embryo of the seeds with partial internal weak physiological conditions can be improved, and the germination capability is improved.
The sources of the catechol, the vitamin B12, the zinc sulfate and the manganese sulfate are not particularly limited in the invention, and the catechol, the vitamin B12, the zinc sulfate and the manganese sulfate can be any conventional commercial products in the field.
The invention provides a preparation method of the seed treatment agent, which comprises the following steps:
(1) mixing zinc sulfate with water to obtain a first solution;
(2) mixing vitamin B12 with the first solution, and dissolving for more than 1.5h at 50-70 ℃ to obtain a second solution;
(3) mixing manganese sulfate with the second solution to obtain a third solution;
(4) and mixing the catechol with the third solution to obtain the seed treating agent.
Mixing zinc sulfate and water to obtain a first solution; then mixing the vitamin B12 with the first solution, heating to dissolve to obtain a second solution. In the invention, the heating and dissolving temperature is preferably 50-70 ℃, and more preferably 60 ℃; the time for heating and dissolving is preferably not less than 1.5h, more preferably not less than 2 h. After the second solution is obtained, mixing manganese sulfate with the second solution to obtain a third solution; and finally, mixing the catechol with the third solution to obtain the seed treating agent.
The preparation method provided by the invention can stabilize the property of the seed treatment agent, does not generate precipitates and has the best use effect.
The invention also provides an application of the seed treating agent or the seed treating agent prepared by the preparation method in promoting corn seed germination. Preferably, the application comprises seed soaking, wherein seed soaking liquid is water diluted liquid of the seed treating agent or the seed treating agent prepared by the preparation method; the dilution multiple of the water diluent is preferably 50-200 times, more preferably 80-150 times, and more preferably 100 times; the seed soaking time is preferably 8-24 hours, and more preferably 12-16 hours.
The seed treatment agent provided by the invention is diluted by 100 times of water to carry out corn seed soaking treatment for more than 12 hours, so that the germination potential of seeds can be improved, the germination potential is increased by 58.42% on average and the germination rate is increased by 33.16% on average; the fresh weight of the overground part of the corn seedling is averagely increased by 26.78 percent compared with the control, and the dry weight is averagely increased by 22.95 percent compared with the control; the fresh weight of the root system is increased by 65.10 percent on average compared with the control, and the dry weight is increased by 34.22 percent on average compared with the control
The technical solution provided by the present invention will be clearly and completely described below with reference to the embodiments. It should be noted that the examples listed below are only some examples of the present invention, and not all examples. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The seed treatment agent for improving the germination of the corn seeds is prepared according to the following steps
(1) Dissolving 30g of zinc sulfate in a proper amount of water, adding VB 1220 mg after the zinc sulfate is fully dissolved, and heating to 60 ℃ to fully dissolve the zinc sulfate for more than 2 hours;
(2) adding 20g of manganese sulfate into the mixture obtained in the step (1), and fully mixing;
(3) adding 20mg of catechol into the mixture obtained in the step (2), and fully dissolving and mixing;
(4) and finally adding water for dissolving to 1000ml, and fully mixing to obtain the seed treatment agent for improving the germination of the corn seeds.
Example 2
The seed treatment agent for improving the germination of the corn seeds is prepared according to the following steps
(1) Dissolving 30g of zinc sulfate in a proper amount of water, adding VB 1215 mg after full dissolution, and heating to 60 ℃ to fully dissolve for more than 2 hours;
(2) adding 15g of manganese sulfate into the mixture obtained in the step (1), and fully mixing;
(3) adding 20mg of catechol into the mixture obtained in the step (2), and fully dissolving and mixing;
(4) and finally adding water for dissolving to 1000ml, and fully mixing to obtain the seed treatment agent for improving the germination of the corn seeds.
Example 3
The seed treatment agent for improving the germination of the corn seeds is prepared according to the following steps
(1) Dissolving 25g of zinc sulfate in a proper amount of water, adding VB 1215 mg after full dissolution, and heating to 60 ℃ to fully dissolve for more than 2 hours;
(2) adding 18g of manganese sulfate into the mixture obtained in the step (1), and fully mixing;
(3) adding 18mg of catechol into the mixture obtained in the step (2), and fully dissolving and mixing;
(4) and finally adding water for dissolving to 1000ml, and fully mixing to obtain the seed treatment agent for improving the germination of the corn seeds.
Example 4
The seed treatment agent for improving the germination of the corn seeds is prepared according to the following steps
(1) Dissolving 20g of zinc sulfate in a proper amount of water, adding VB 1220 mg after the zinc sulfate is fully dissolved, and heating to 60 ℃ to fully dissolve for more than 2 hours;
(2) adding 20g of manganese sulfate into the mixture obtained in the step (1), and fully mixing;
(3) adding 20mg of catechol into the mixture obtained in the step (2), and fully dissolving and mixing;
(4) and finally adding water for dissolving to 1000ml, and fully mixing to obtain the seed treatment agent for improving the germination of the corn seeds.
Example 5
The seed treatment agent for improving the germination of the corn seeds is prepared according to the following steps
(1) Dissolving 20g of zinc sulfate in a proper amount of water, adding VB 1215 mg after full dissolution, and heating to 60 ℃ to fully dissolve for more than 2 hours;
(2) adding 15g of manganese sulfate into the mixture obtained in the step (1), and fully mixing;
(3) adding 15mg of catechol into the mixture obtained in the step (2), and fully dissolving and mixing the mixture;
(4) and finally adding water for dissolving to 1000ml, and fully mixing to obtain the seed treatment agent for improving the germination of the corn seeds.
Example 6
The application effect test is as follows:
experiments are carried out in the key laboratory of ecological agriculture and biotechnology of Huaiyin teachnological lake, Jiangsu province, Huaizu lake, in 2018 in 3 and 4 months, the corn variety is Suyu No. 42 (the seed is provided by agricultural science institute of Huaian city), the corn seed is firstly soaked in 1.0% sodium hypochlorite solution for 2 hours for disinfection, then washed with clear water for 2-3 times for later use, and the treatment is respectively: clean water (control), 100-fold dilution of the novel treatment agent (5 examples), 250mg/ml zinc sulfate, 20mg/ml manganese sulfate, 0.20mg/ml catechol, 0.20mg/ml VB12 solution each 100ml for 12 hours.
Each treatment was repeated 3 times. The seeds were sown evenly in petri dishes with absorbent paper, 20 seeds per petri dish. And covering a layer of absorbent paper on the seeds, keeping the absorbent paper wet all the time during the culture period, and reducing the watering amount after the seeds germinate. And 5d, recording the germination number of the seeds, calculating the germination potential, and 7d, recording the germination number of the seeds, and calculating the germination rate. And 15d, respectively measuring the fresh weight and the dry weight of the overground part of the seedling and the fresh weight and the dry weight of the root system. The test results are shown in tables 1 and 2.
TABLE 1 Effect of different treatments on corn seed Germination
Figure BDA0002025921230000061
Figure BDA0002025921230000071
TABLE 2 Effect of different treatments on the vigor of maize seedlings
Figure BDA0002025921230000072
From the results in table 1, it is seen that the corn seeds treated with the treatment agent of the present invention diluted 100 were the most effective, and the germination potential of the seeds was increased by 58.42% on average and the germination rate was increased by 33.16% on average. Are superior to other treatments.
As can be seen from the results in table 2, the fresh weight of the aerial parts of the maize seedlings treated with the treatment agent of the present invention diluted 100 was increased by 26.78% on average and the dry weight was increased by 22.95% on average. The fresh weight of the root system is increased by 65.10 percent on average compared with the control, and the dry weight is increased by 34.22 percent on average compared with the control.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The seed treatment agent for promoting germination of corn seeds is characterized by taking water as a dispersing agent and comprising the following dispersoids in parts by mass: 15-25 parts of catechol, 1215-25 parts of vitamin B, 15000-30000 parts of zinc sulfate and 15000-25000 parts of manganese sulfate.
2. The seed treatment agent according to claim 1, wherein the seed treatment agent uses water as a dispersant, and comprises the following dispersoids in parts by mass: 18-22 parts of catechol, 1218-22 parts of vitamin B, 18000-22000 parts of zinc sulfate and 18000-22000 parts of manganese sulfate.
3. The seed treatment agent according to claim 2, wherein the seed treatment agent uses water as a dispersant, and comprises the following dispersoids in parts by mass: 20 parts of catechol, 1220 parts of vitamin B, 20000 parts of zinc sulfate and 20000 parts of manganese sulfate.
4. The seed treatment agent according to any one of claims 1 to 3, wherein the seed treatment agent comprises water as a dispersion system and dispersoids at the following concentrations: 15-20 mg/L of catechol, 1215-20 mg/L of vitamin B, 20-30 g/L of zinc sulfate and 15-20 g/L of manganese sulfate.
5. The seed treatment of claim 4, wherein the seed treatment is in the form of a water dispersion comprising the following concentrations of dispersoids: 20mg/L of catechol, 1220 mg/L of vitamin B, 20g/L of zinc sulfate and 20g/L of manganese sulfate.
6. A method for producing the seed treatment agent according to any one of claims 1 to 5, characterized by comprising the steps of:
(1) mixing zinc sulfate with water to obtain a first solution;
(2) mixing vitamin B12 with the first solution, and dissolving for more than 1.5h at 50-70 ℃ to obtain a second solution;
(3) mixing manganese sulfate with the second solution to obtain a third solution;
(4) and mixing pyrocatechol with the third solution to obtain the seed treatment agent.
7. The preparation method according to claim 6, wherein the dissolution time in step (2) is not less than 2 h.
8. Use of the seed treatment agent of any one of claims 1 to 5 or the seed treatment agent prepared by the preparation method of any one of claims 6 to 7 for promoting germination of corn seeds.
9. The use according to claim 8, wherein the method for promoting germination of corn seeds comprises seed soaking, wherein the seed soaking liquid is water diluted liquid of the seed treatment agent of any one of claims 1 to 5 or the seed treatment agent prepared by the preparation method of any one of claims 6 to 7; the dilution times of the water diluent are 50-200 times; the seed soaking time is 8-24 h.
10. The use according to claim 9, wherein the seed soaking time is 12-16 h.
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CN111802017A (en) * 2020-08-12 2020-10-23 黑龙江八一农垦大学 Method for improving stress resistance of corn seeds by using exogenous vitamins
CN113906863A (en) * 2021-11-01 2022-01-11 华中农业大学 Crop seed cladding or pelleting preparation method based on straw biochar and catechol compounding
CN113924845A (en) * 2021-11-01 2022-01-14 华中农业大学 Catechol-based seed coating method
CN115245175B (en) * 2022-08-30 2023-10-10 淮阴师范学院 Seed treating agent and preparation method and application thereof

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