CN105875611A - Plant salt resisting conditioning agent and preparation method thereof - Google Patents
Plant salt resisting conditioning agent and preparation method thereof Download PDFInfo
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- CN105875611A CN105875611A CN201610364846.2A CN201610364846A CN105875611A CN 105875611 A CN105875611 A CN 105875611A CN 201610364846 A CN201610364846 A CN 201610364846A CN 105875611 A CN105875611 A CN 105875611A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of plant growth conditioning agents, and relates to a plant salt resisting conditioning agent. The plant salt resisting conditioning agent is prepared from porphyra polysaccharides with the concentration of 100-1,000 mg/L, salicylic acid with the concentration of 0 mg/L or 100-200 mg/L, brassinolide with the concentration of 0 mg/L or 0.01-0.1 mg/L, glycine betaine with the concentration of 0 mg/L or 100-1,000 mg/L, spermidine with the concentration of 0 mg/L or 10-100 mg/L, a surfactant with the concentration of 0 mg/L or 1,000-20,000 mg/L and water, wherein the water serves as a solvent, and low-molecular-weight porphyra polysaccharides with the molecular weight of 1 k-50 k Da are adopted as the porphyra polysaccharides. Accordingly, novel application of the porphyra polysaccharides is supplied, and the porphyra polysaccharides can be used for preparing the plant salt resisting conditioning agent; meanwhile, by compounding the porphyra polysaccharides with the biological source induced resisting substances such as salicylic acid, brassinolide, glycine betaine and spermidine to prepare the salt resisting conditioning agent, the comprehensive resistance of plants on salt stress can be improved, and normal growth and development of the plants are guaranteed; according to the salt resisting conditioning agent, the components are simple, the raw materials are easy to obtain, the cost is low, the effect is significant, and environmental friendliness is achieved.
Description
Technical field:
The invention belongs to plant growth regulator technical field, be specifically related to a kind of plant anti-salt regulator.
Background technology:
Thallus Porphyrae is a kind of large-scale economic red algae, belongs to Rhodophyceae, Bangia fuscopurpurea section, frond in the most brown, red or
Purple, primary growth, in Intertidal zone, shallow sea, has stronger fertility and environment resistance.Coastal area of china
Area has carried out artificial culture, and at the beginning of 21 century, China's Thallus Porphyrae yield leaps to No. 1 in the world.Thallus Porphyrae contains
Porphyra Polysaccharide is one of Thallus Porphyrae main active substances, its have immunoregulatory activity, promote cell-proliferation activity and
Numerous feature such as antioxidant activity, is widely used at food and industrial circle, and Porphyra Polysaccharide can
Promote plant growing, at plant disease-resistant and degeneration-resistant aspect, also there is huge application potential.Salicylic acid (SA)
It is induction plant disease-resistant and degeneration-resistant signal of interest molecule, it is possible to increase plant protection enzymatic activity, increases infiltration
Auto-regulator and its secondary metabolites content, thus improve disease resistance and the resistance of plant;Brassinosteroid
(BR) it is a kind of steroid compound that plant is had growth regulating effect, it is possible to alleviate environment stress to planting
The injury effect of thing seedling, it, as a kind of novel plant hormone, has extensively on stress resistance of plant improving
Application;Glycine betaine (GB) is a kind of quaternary ammonium type water-soluble alkaloid, is also a kind of atoxic Osmoregulation
Material, it has obtained increasing attention in plant stress-resistance;Spermidine (Spd) is considered as similar the
The active substance of two couriers, plays an important role to the resistance of induction plant;Above-mentioned four kinds of materials are the most nontoxic,
Harmless, pollution-free, it is possible to induction plant produces resistance, is that the biogenic chemistry of environmental protection lures anti-material.
China's salinized soil area is wide, of a great variety, and the modern salinized soil of major part is plantation farming region at present,
It mostly is the important grain of China, cotton, oil crop production base, but crop yield is relatively low.Semen Tritici aestivi is that China is second largest
Cereal crops, its yield and quality is also by the serious restriction of the soil salinization.Strengthen Semen Tritici aestivi saline-alkaline tolerance pair
Ensure that improving yield of wheat stable yields, Ensuring Food Safety have important practical significance and using value;Practice has
Chinese patent CN1290483A, CN1943340A etc. disclose Semen Tritici aestivi anti-salt regulator, its key component
There are gibberellins, salicylic acid aminopolysaccharide, sorbitol and a mannitol etc., but at present with Porphyra Polysaccharide as raw material
Anti-salt agent have not been reported, and Porphyra Polysaccharide is simple and easy to get, with low cost, green natural, resists for crop
Salt has important economic implications.
Summary of the invention:
It is an object of the invention to the shortcoming overcoming prior art to exist, it is provided that a kind of plant anti-salt regulator,
Plant seed or the multinomial physical signs of seedling can be strengthened, improve multiple activities of antioxidant enzymes, improve plant
The ability of opposing salt stress.
To achieve these goals, the composition of the plant anti-salt regulator that the present invention relates to includes Porphyra Polysaccharide,
The proportioning of described Porphyra Polysaccharide is 100-1000mg/L, and water is as solvent.
Further, described plant anti-salt regulator also includes salicylic acid, brassinosteroid, glycine betaine, Asia
One or more in spermine, surfactant.
Further, described salicylic acid proportioning is 100-200mg/L, and brassinosteroid proportioning is 0.01-0.1
Mg/L, glycine betaine proportioning is 100-1000mg/L, and spermidine proportioning is 10-100mg/L, surfactant
Proportioning is 1000-20000mg/L, and water is as solvent.
Described Porphyra Polysaccharide is the low-molecular-weight Porphyra Polysaccharide of molecular weight 1k-50kDa;
The described surfactant related to is in TWEEN Series, Span series, OP series and/or NP series
The mixture of one or more.
The preparation method of described plant anti-salt regulator:
Being dissolved in distilled water by Porphyra Polysaccharide, the activity making Porphyra Polysaccharide is 100-1000mg/L, stirring
Uniformly, it is configured to Porphyra Polysaccharide solution and i.e. prepares described anti-salt regulator;
Further, also can add in above-mentioned Porphyra Polysaccharide solution salicylic acid, brassinosteroid, glycine betaine,
Spermidine, in one or more, the activity making each component is salicylic acid 100-200mg/L, Brassica campestris L
Element lactone 0.01-0.1mg/L, glycine betaine 100-1000mg/L, spermidine 10-100mg/L, finally stir
Uniformly proportioning makes combination solution;
Further, also can add surfactant in described combination solution, stir, described surface
Surfactant concentration is 1000-20000mg/L, and gained solution can use as spray.
The using method of described plant anti-salt regulator is: spray plant seedlings/irrigate, or to planting
Species is sowed after carrying out seed soaking 6-12h.
Preferably, plant seedlings is sprayed.
Action principle: under salt stress, can accumulate a large amount of Na in plant+, K+Absorb and reduce, cause higher
Na+/K+。K+It is monovalent cation important in plant, is not only chlorophyllous constituent, also may be used
To participate in internal important metabolism, therefore, K in plant leaf as zymoexciter+Content reduces, and can suppress light
The transmission of chemical reaction center electron and the activity of photosynthetic enzyme.
Porphyra Polysaccharide can regulate plant in ion transport to improve salt resistance of plants, on the one hand, Thallus Porphyrae is many
Sugar is by excess Na+It is stored in root and Lao Ye, alleviates Na+Young leaves is coerced, to ensure the life of plant young leaves
Reason function.On the other hand, Porphyra Polysaccharide can induce plant by the Na of excess in plant cytoplasm+Ion turns
It is transported in extracellular or vacuole, K higher in maintaining plant cell+Content and relatively low Na+/K+.Purple
Dish polysaccharide is by K in regulation ion transport guarantee plant+Content, it is possible to ensure the electricity of photochemical reaction center
Son transmission, alleviates high concentration Na+The pore caused is closed, and improves Plants under Salt Stress photosynthesis, and then
Improve salt resistance of plants.
Described plant anti-salt regulator can extensively be tried out in various plants, including monocotyledon, dicotyledonous plants
Thing, commonly used industrial crops have Semen Tritici aestivi, seeding corn and other crops and the vegetable such as Fructus Cucumidis sativi, Brassica campestris L.
The invention provides Porphyra Polysaccharide and improve the plant new principle to salt stress ability, therefore, utilize this
Principle is prepared plant anti-salt Related product and is belonged to the content that the present invention is open and protects.
Beneficial effect:
The present invention is compared with prior art and product, it is provided that the new opplication of Porphyra Polysaccharide, can be used for preparation and plants
Thing anti-salt regulator, simultaneously that Porphyra Polysaccharide is raw with salicylic acid, brassinosteroid, glycine betaine and spermidine etc.
Thing source lures anti-material to compound, and makes anti-salt regulator, it is possible to increase the plant comprehensive opposing to salt stress
Power, it is ensured that plant normal growth is grown;Selected raw material Thallus Porphyrae abundance, with low cost, environmental friendliness;
The method preparing regulator is simple, it is simple to popularization and application.
Accompanying drawing illustrates:
Fig. 1 different organs of wheat Na+Content;
Fig. 2 different organs of wheat Na+/K+Ratio;
Fig. 3 Semen Tritici aestivi Na+/H+ transporter gene is expressed, and wherein 3-A is cell membrane Na+/H+Transporter gene
SOS;3-B is tonoplast Na+/H+Transporter gene NHX.
Detailed description of the invention:
Below by embodiment, the present invention is described in further detail, but the present invention is not limited to following reality
Execute mode.
Embodiment 1:
The low-molecular-weight Porphyra Polysaccharide that molecular weight is 1-50kDa is dissolved in distilled water by the present embodiment, makes Thallus Porphyrae
Polysaccharide concentration is 100-1000mg/L, prepares anti-salt regulator A;Or be separately added in anti-salt regulator A
Salicylic acid, brassinosteroid, glycine betaine and/spermidine any of which or multiple, concrete concentration is bigcatkin willow
Acid 0mg/L or 100-200mg/L, brassinosteroid 0mg/L or 0.01-0.1mg/L, glycine betaine 0mg/L
Or 100-1000mg/L, spermidine 0mg/L or 10-100mg/L, prepare anti-salt regulator B;Or again to
Being separately added into surfactant in anti-salt regulator A or anti-salt regulator B, tween system selected by surfactant
The mixture of one or more in row, Span series, OP series and/or NP series, makes surfactant
Concentration is 1-20g/L, prepares anti-salt regulator C;Above-mentioned anti-salt regulator A, anti-salt regulator B and anti-
Salt regulator C all can be effectively improved the saline-alkaline tolerance of plant, promotes that it produces.
Embodiment 2:
The present embodiment first weighs the Porphyra Polysaccharide that 100mg molecular weight is 5kDa and is dissolved in 800ml distilled water
In, add 1000mg tween 20 and stir, being finally settled to 1L with distilled water, stir
Rear prepared described anti-salt regulator.
Embodiment 3:
The present embodiment first weighs the Porphyra Polysaccharide that 100mg molecular weight is 5kDa and is dissolved in 800ml distilled water
In prepare Porphyra Polysaccharide solution, then weigh 100mg salicylic acid and be dissolved in above-mentioned Porphyra Polysaccharide solution, then add
Enter 1000mg tween 20 and stir, being finally settled to 1L with distilled water, after stirring, preparing institute
State anti-salt regulator.
Embodiment 4:
The present embodiment first weighs the Porphyra Polysaccharide that 100mg molecular weight is 5kDa and is dissolved in 800ml distilled water
In prepare Porphyra Polysaccharide solution, then weigh 0.01mg brassinosteroid and be dissolved in above-mentioned Porphyra Polysaccharide solution,
It is subsequently adding 5000mg Arlacel-60 and stirs, being finally settled to 1L with distilled water, after stirring
Prepare described anti-salt regulator.
Embodiment 5:
The present embodiment first weighs the Porphyra Polysaccharide that 100mg molecular weight is 50kDa and is dissolved in 800ml distilled water
In prepare Porphyra Polysaccharide solution, then weigh 10mg spermidine and be dissolved in above-mentioned Porphyra Polysaccharide solution, then add
Enter 5000mg OP-7 and stir, being finally settled to 1L with distilled water, preparing described after stirring
Anti-salt regulator.
Embodiment 6:
The present embodiment first weighs the Porphyra Polysaccharide that 1000mg molecular weight is 5kDa and is dissolved in 800ml distilled water
In prepare Porphyra Polysaccharide solution, then weigh 100mg glycine betaine and be dissolved in above-mentioned Porphyra Polysaccharide solution, then add
Enter 5000mg NP-7 and stir, being finally settled to 1L with distilled water, preparing described after stirring
Anti-salt regulator.
Checking test:
The present invention is as a example by Semen Tritici aestivi, to being in wheat seedling embodiment 2-6 of Seedling Stage the anti-salt prepared
Regulator carries out sprinkling process, and specific experiment is as follows:
Experimental technique: by wheat seed sterilization, immersion, accelerating germination, treat that seed just shows money or valuables one carries unintentionally, select full, big
Little homogeneous wheat seed is sowed in culture dish, 30, every ware, with Hoagland (Gram suddenly) nutrition
Liquid is cultivated in illumination box, and condition of culture is 25/20 DEG C (day night), intensity of illumination 800 μm ol/m2/s、
Periodicity of illumination 14/10h (day night), relative humidity 65+5%;When wheat seedling growth to 21 heart stage of leaf,
With the anti-salt regulator prepared in embodiment 2-6, wheat seedlings blade is sprayed, makes blade the most moistening,
But liquid does not flows down, negative control group spray is with the clear water containing same concentrations surfactant of equivalent;24h
After wheat seedling is placed in salt stress 10d, then measure wheat seedlings blade indices.
Experimental result: after 1. salt stress processes wheat seedling 2h, 5d and 10d, take Semen Tritici aestivi difference group respectively
Knit and carry out ion measurement.Take Wheat Seedling Roots, stem (plant root is to lowermost position blade), Lao Ye (plant under
Position blade) and young leaves (the up-to-date blade of plant tip), clean with distilled water flushing.105 DEG C of 30min that complete,
Dry to constant weight for 70 DEG C.For elementary analysis.From the results, it was seen that Porphyra Polysaccharide can effectively regulate Na+
Transhipment distribution in Semen Tritici aestivi body, by excess Na+It is stored in root and Lao Ye, alleviates Na+Young leaves is coerced,
Ensure the physiological function (see Fig. 1) of wheat seedling.
2. Porphyra Polysaccharide can also regulate K+Transhipment distribution in Semen Tritici aestivi body, maintains wheat seedling stem and young leaves
In relatively low Na+/K+Value, to ensure Semen Tritici aestivi normal physiological function (see Fig. 2).
3., after salt stress processes 10d, wheat seedlings blade stomatal conductance is remarkably decreased (P < 0.05), causes
Photosynthetic rate significantly reduces, and has had a strong impact on the photosynthetic rate of plant, and then have impact on the normal physiology of plant
Function.Porphyra Polysaccharide process group stomatal conductance can maintain matched group level, thus also ensure that photosynthetic speed
Rate (is shown in Table 1).
Table 1 wheat seedling stomatal conductance and photosynthetic rate
4. the present embodiment uses fluorescent dye SYBR GreenI, uses 2-ΔΔCTMethod, to salt anti-in Semen Tritici aestivi
Stress-related genes expression under Porphyra Polysaccharide processes carries out relative quantification research, many to inquire into Thallus Porphyrae
The molecule mechanism of the sugar anti-salt action of inducing wheat.Found that SOS and NHX gene expression is equal under salt stress
Present and first raise the trend lowered afterwards.Process through Porphyra Polysaccharide induction, after SOS gene expression presents first enhancing
The trend (see Fig. 3-A) weakened, and NHX gene expression is obviously enhanced (see Fig. 3-B) in the salt stress later stage,
Illustrate that Porphyra Polysaccharide can regulate ion transporter gene and express, coordinate in Wheat Seedling Under Salt Stress body
Na+Transhipment, by the Na of excess+Discharge outside Cytoplasm or be transported in vacuole, it is ensured that plant cell is normally given birth to
Reason function, thus improve salt resistance of plants.
Use the anti-salt agent of embodiment of the present invention 2-6 gained, be applied on other plant, it may have good
Anti-salt effect, the principle that principle draws with described confirmatory experiment is consistent.
Claims (9)
1. Porphyra Polysaccharide application in plant anti-salt regulator.
Apply the most as claimed in claim 1, it is characterised in that: described Porphyra Polysaccharide molecular weight is molecule
Amount is 1k-50kDa.
3. based on the application described in claim 1 or 2, a kind of plant anti-salt regulator is many including Thallus Porphyrae
Sugar, the final concentration of 100-1000mg/L that described Porphyra Polysaccharide is dissolved in water.
4. a kind of plant anti-salt regulator as claimed in claim 3, it is characterised in that: also include bigcatkin willow
One or more in acid, brassinosteroid, glycine betaine, spermidine, surfactant.
5. a kind of plant anti-salt regulator as described in claim 3 or 4, it is characterised in that: described respectively
The activity of component is salicylic acid 100-200mg/L, brassinosteroid 0.01-0.1mg/L,
Glycine betaine 100-1000mg/L, spermidine 10-100mg/L.
6. a kind of plant anti-salt regulator as claimed in claim 4, it is characterised in that: live in described surface
Property agent be TWEEN Series, Span series, OP series and/or NP series in one or more
Mixture.
7. a kind of plant anti-salt regulator as claimed in claim 3, its preparation method is: Thallus Porphyrae is many
Sugar is dissolved in distilled water, and the activity making Porphyra Polysaccharide is 100-1000mg/L, and stirring is all
Even, it is configured to Porphyra Polysaccharide solution and i.e. prepares described anti-salt regulator.
8. preparation method as claimed in claim 7, it is characterised in that: in described Porphyra Polysaccharide solution
Add salicylic acid, brassinosteroid, glycine betaine, spermidine, in one or more, make each
The activity of component is salicylic acid 100-200mg/L, brassinosteroid 0.01-0.1mg/L,
Glycine betaine 100-1000mg/L, spermidine 10-100mg/L, the proportioning that stirs is made compounding
Solution.
9. preparation method as claimed in claim 8, it is characterised in that: add in described combination solution
Surfactant, stirs, and described surfactant concentration is 1000-20000mg/L.
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