CN110521327A - A method of alleviating Lead sweet and the sprouting of bitter buckwheat seed and growth of seedling are injured - Google Patents
A method of alleviating Lead sweet and the sprouting of bitter buckwheat seed and growth of seedling are injured Download PDFInfo
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- CN110521327A CN110521327A CN201910904121.1A CN201910904121A CN110521327A CN 110521327 A CN110521327 A CN 110521327A CN 201910904121 A CN201910904121 A CN 201910904121A CN 110521327 A CN110521327 A CN 110521327A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of alleviation Lead sweets to the method for the sprouting of bitter buckwheat seed and growth of seedling injury, the following steps are included: choosing bitter buckwheat seed of the appearance without scar, full seed, it dries, is then carried out disinfection using thimerosal in the sun, finally use distilled water flushing；Seed after flushing is put into Gibberellins solution and is submerged, constant temperature seed soaking；Seed after seed soaking is evenly laid out on filter paper, it is placed in shading culture under aseptic condition, obtains bitter buckwheat seedling；The neat strong sprout of growing way is chosen, plantation to loose smooth soil, root mulching soil, then every plant of seedling applies mixed fertilizer.The present invention can effectively promote bitter buckwheat seedling vigor under the conditions of Lead sweet by the seed soaking by bitter buckwheat seed in Gibberellins solution, to resist heavy metal lead stress, improve the germination percentage of bitter buckwheat seed.
Description
Technical field
The invention belongs to field of agricultural production technologies, and in particular to a kind of alleviation Lead sweet is sprouted to bitter buckwheat seed and seedling
Grow the method for injury.
Background technique
The related research table of bitter buckwheat nutritive value with higher, agriculture in recent years, medicine and food science etc.
Bright, its nutritive value shelter of bitter buckwheat has first of cereal crops, and not only rich in nutrition content, nutritive value are high, but also contain other
The extraordinary microelement and medicinal ingredient that cereal crops do not have, to modern " ciril disease " and nearly all person in middle and old age's cardiovascular and cerebrovascular
Disease has prevention and treatment function, thus the attention by various countries.New ecology potential is presented in bitter buckwheat production and health food development
Head.
China is the big producer of bitter buckwheat, bitter buckwheat impoverishment tolerant, freeze-resistant and the cultivation advantages such as growth cycle is short.However it is bitter
Buckwheat per mu yield is extremely low, is unable to satisfy growing demand, it is desirable to improve bitter buckwheat total output, it is most important to increase cultivated area.But
It is that different degrees of heavy metal pollution is all received due to China's major part soil, soil is difficult to provide bitter buckwheat suitable growth item
Part.Want the nutritive value and yield of raising bitter buckwheat, we can be by improving bitter buckwheat to the resistance or alleviation of heavy metal
Heavy metal, which grows bitter buckwheat, coerces, and improves bitter buckwheat yield and nutritive value to reach.It looks for and alleviates heavy metal pollution to bitter buckwheat
The method of growth stress can play the role of vital for the development of modern agriculture.
The main method for alleviating the injury of plant heavy metal stress has mineral element, plant growth regulator, microorganism etc., plants
Object growth regulator improves resistance by adjusting the upgrowth situation of plant, such as applies Exogenous Abscisic Acid, can restore a huge sum of money
Belong to seed under Hg2+ stress and sprouts vigor.Gibberellin can break seed dormancy phase, promote the sprouting of seed and the growth of seedling, most
Apparent biological effect is exactly the plant growth for promoting cell elongation to make.Gibberellin can promote the conjunction of RNA and protein
At reducing the activity of indoleacetic acid oxidase, improve the activity of protease, exist in the generating process of plant lateral roots and adventitious root
There is apparent inhibiting effect, these functions of gibberellin can promote the growth and development of plant, and gibberellin is alleviating bitter buckwheat
Lead sweet sprouts seed and seedling development injury is on the knees of the gods.
Summary of the invention
For the above-mentioned problems in the prior art, the present invention provide a kind of alleviation Lead sweet bitter buckwheat seed is sprouted and
The method of growth of seedling injury can effectively promote Lead sweet item by the seed soaking by bitter buckwheat seed in Gibberellins solution
Bitter buckwheat seedling vigor under part improves the germination percentage of bitter buckwheat seed to resist heavy metal lead stress.
To achieve the above object, the technical solution adopted by the present invention to solve the technical problems is: providing a kind of alleviation lead
Coerce the method to the sprouting of bitter buckwheat seed and growth of seedling injury, comprising the following steps:
(1) bitter buckwheat seed of the appearance without scar, full seed is chosen, in the sun sunning 2~3 days, daily 5~6h, so
It is carried out disinfection afterwards using thimerosal, finally uses distilled water flushing；
(2) seed after rinsing step (1), which is put into concentration 5~100mg/L Gibberellins solution, to be submerged, at 23~28 DEG C
At a temperature of constant temperature seed soaking 8~for 24 hours；
(3) seed after step (2) seed soaking is evenly laid out on filter paper, it is placed under aseptic condition, 20~25 DEG C of shadings
Culture 8~10 days, watering 2 times, obtain bitter buckwheat seedling daily；
(4) the neat strong sprout of growing way in selecting step (3), plantation to loose smooth soil, root mulching soil 3~
4cm, then every plant of seedling applies 0.3~1.2kg mixed fertilizer.
Further, use concentration for the disinfection liquid disinfectant 2h of 0.1wt%, then use distilled water flushing when disinfection in step (1)
3~5 times.
Further, thimerosal is potassium permanganate, formaldehyde or hydrogen peroxide solution.
Further, Gibberellins solution includes following parts by weight of component: 10~15 parts of gibberellin fermented liquid, sodium nitrate 80~
110 parts, 90~150 parts of ammonium sulfate, 90~150 parts of potassium sulfate, 80~110 parts of calcium superphosphate, 15~20 parts of ammonium molybdate and water
1100~1400 parts.
Further, Gibberellins solution includes following parts by weight of component: 13 parts of gibberellin fermented liquid, 90 parts of sodium nitrate, sulfuric acid
120 parts of ammonium, 120 parts of potassium sulfate, 90 parts of calcium superphosphate, 18 parts of ammonium molybdate and 1300 parts of water.
Further, gibberellin fermented liquid is prepared by the following method to obtain: gibberellin is fermented 2 under the conditions of 28~30 DEG C
~3 days, then extracted after being concentrated, obtain gibberellin fermented liquid.
Further, mixed fertilizer described in step (3) be organic fertilizer, urea, potassium oxide and calcium superphosphate by weight 30~
The fertilizer that 60:0.1~0.4:2~6:3~7 mix.
Further, the weight ratio of organic fertilizer, urea, potassium oxide and calcium superphosphate is 40:0.3:4:5.
In conclusion the present invention has following advantages:
1, it under the conditions of Lead sweet, is handled using the seed soaking nursery that this method carries out bitter buckwheat seed, the lead side of body can be effectively relieved
Compel to sprout bitter buckwheat seed and the injury of growth of seedling, percentage of seedgermination, germinating energy and the germination improved under bitter buckwheat Lead sweet refer to
Number can significantly improve germination percentage after gibberellin seed soaking；Reduce adventitious root quantity, free aminoacid content and superoxide dismutase
Enzymatic activity is to reach close to normal value.Wherein the bitter buckwheat growing way after the seed soaking of 40mg/L gibberellin and physical signs are closest to normally
Bitter buckwheat seed is sprouted, bitter buckwheat percentage of seedgermination improves 9%, and adventitious root quantity reduces 20%, and superoxide dismutase activity is reduced
76%, free aminoacid content reduces 29%.
2, gibberellin can break seed dormancy phase, promote the sprouting of seed and the growth of seedling, most apparent biology
Effect is exactly the plant growth for promoting cell elongation to make.Gibberellin can promote the synthesis of RNA and protein, reduce indoles second
The activity of acid oxidase improves the activity of protease, is also having apparent inhibition in the generating process of plant lateral roots and adventitious root
Effect, these functions of gibberellin can promote the growth and development of plant.Germination percentage is significantly improved after gibberellin seed soaking；It reduces
Adventitious root quantity, free aminoacid content and superoxide dismutase activity are to reach close to normal value.
3, mixed fertilizer also is applied to seedling in the present invention, it is micro- to provide organic substance and N P and K etc. in seedling production process
Secondary element promotes the growth of seedling, alleviates injury of the Lead sweet to bitter buckwheat seed sprouting and growth of seedling, subsequent to adapt to seedling
Growth.
Detailed description of the invention
Fig. 1 is that Gibberellins solution is soaked seed on germination percentage influence schematic diagram；
Fig. 2 is that Gibberellins solution is soaked seed on root long influence schematic diagram；
Fig. 3 is that Gibberellins solution is soaked seed on stem length influence schematic diagram；
Fig. 4 is that Gibberellins solution is soaked seed on soluble protein content influence schematic diagram；
Fig. 5 is that Gibberellins solution is soaked seed to Influences of MDA Content schematic diagram；
Fig. 6 is that Gibberellins solution is soaked seed on free proline content influence schematic diagram；
Fig. 7 is that Gibberellins solution is soaked seed on superoxide dismutase activity influence schematic diagram.
Specific embodiment
Embodiment 1
A method of alleviating Lead sweet and the sprouting of bitter buckwheat seed and growth of seedling injured, comprising the following steps:
(1) bitter buckwheat seed of the appearance without scar, full seed is chosen, in the sun sunning 2~3 days, daily 5~6h, so
Concentration is used to sterilize 2h for the formaldehyde of 0.1wt% afterwards, then with distilled water flushing 3~5 times；
(2) seed after rinsing step (1), which is put into concentration 20mg/L Gibberellins solution, to be submerged, in 23~28 DEG C of temperature
Lower constant temperature seed soaking 8h；Wherein, Gibberellins solution includes following parts by weight of component: 10 parts of gibberellin fermented liquid, 80 parts of sodium nitrate, sulphur
90 parts of sour ammonium, 900 parts of potassium sulfate, 80 parts of calcium superphosphate, 15 parts of ammonium molybdate and 1100 parts of water；
(3) seed after step (2) seed soaking is evenly laid out on filter paper, it is placed under aseptic condition, 20~25 DEG C of shadings
Culture 8~10 days, watering 2 times, obtain bitter buckwheat seedling daily；
(4) the neat strong sprout of growing way in selecting step (3), plantation to loose smooth soil, root mulching soil 3~
4cm, then every plant of seedling applies 0.3kg mixed fertilizer.Wherein, mixed fertilizer is organic fertilizer, urea, potassium oxide and calcium superphosphate by weight
Measure the fertilizer mixed than 30:0.1:2:3.
Embodiment 2
A method of alleviating Lead sweet and the sprouting of bitter buckwheat seed and growth of seedling injured, comprising the following steps:
(1) bitter buckwheat seed of the appearance without scar, full seed is chosen, in the sun sunning 2~3 days, daily 5~6h, so
Concentration is used to sterilize 2h for the potassium permanganate of 0.1wt% afterwards, then with distilled water flushing 3~5 times；
(2) seed after rinsing step (1), which is put into concentration 40mg/L Gibberellins solution, to be submerged, in 23~28 DEG C of temperature
Lower constant temperature seed soaking 16h；Wherein, Gibberellins solution includes following parts by weight of component: 13 parts of gibberellin fermented liquid, 90 parts of sodium nitrate,
120 parts of ammonium sulfate, 120 parts of potassium sulfate, 90 parts of calcium superphosphate, 18 parts of ammonium molybdate and 1300 parts of water；
(3) seed after step (2) seed soaking is evenly laid out on filter paper, it is placed under aseptic condition, 20~25 DEG C of shadings
Culture 8~10 days, watering 2 times, obtain bitter buckwheat seedling daily；
(4) the neat strong sprout of growing way in selecting step (3), plantation to loose smooth soil, root mulching soil 3~
4cm, then every plant of seedling applies 0.8kg mixed fertilizer.Wherein, mixed fertilizer is organic fertilizer, urea, potassium oxide and calcium superphosphate by weight
Measure the fertilizer mixed than 40:0.3:4:5.
Embodiment 3
A method of alleviating Lead sweet and the sprouting of bitter buckwheat seed and growth of seedling injured, comprising the following steps:
(1) bitter buckwheat seed of the appearance without scar, full seed is chosen, in the sun sunning 2~3 days, daily 5~6h, so
Use concentration for the dioxygen water sterilization 2h of 0.1wt% afterwards, then with distilled water flushing 3~5 times；
(2) seed after rinsing step (1), which is put into concentration 100mg/L Gibberellins solution, to be submerged, in 23~28 DEG C of temperature
Spend lower constant temperature seed soaking for 24 hours；Wherein, Gibberellins solution includes following parts by weight of component: 15 parts of gibberellin fermented liquid, sodium nitrate 110
Part, 150 parts of ammonium sulfate, 150 parts of potassium sulfate, 110 parts of calcium superphosphate, 20 parts of ammonium molybdate and 1400 parts of water；
(3) seed after step (2) seed soaking is evenly laid out on filter paper, it is placed under aseptic condition, 20~25 DEG C of shadings
Culture 8~10 days, watering 2 times, obtain bitter buckwheat seedling daily；
(4) the neat strong sprout of growing way in selecting step (3), plantation to loose smooth soil, root mulching soil 3~
4cm, then every plant of seedling applies 1.2kg mixed fertilizer.Wherein, mixed fertilizer is organic fertilizer, urea, potassium oxide and calcium superphosphate by weight
Measure the fertilizer mixed than 60:0.4:6:7.
Experimental example
" the river buckwheat No.1 " weak using Drought sensitivity resistance is as material to be tested, if selecting uniformly full kind of dry particl
Son, the liquor potassic permanganate soaking disinfection 2h for the use of concentration being 0.1wt%, then with distilled water flushing 3 times；Seed sprouting box is led to
Alcohol wipe disinfection is crossed, double-layer filter paper is then placed in, the CK group of blank control is sprouted box and infiltrated using pure water, other 6 experiments
Group sprouts box and is all made of the acetic acid lead solution infiltration that concentration is 800mg/L, and each group processing mode is shown in Table 1；It is again that gibberellin is molten
Seed after vacuole kind 12h is placed on filter paper, 120, every box, is covered in the shading incubator for being put into 25 DEG C after box cover, is poured daily
Water 2 times, and each processing sets 3 repetitions；It after 6 days, does not germinate within seed continuous two days, is denoted as germination and stops, processing is recorded
Data；6th, 9,11 day difference sampling measurement root long stem length level-one adventitious root quantity.Measure germination percentage, root long, stem length, solvable
Property protein content, mda content, free proline content and superoxide dismutase activity, result are shown in Fig. 1~7 respectively.
Wherein, the amount of culture solution is that inclination solution does not flow, and surface flashing light is standard culture.
The processing of 1 each group bitter buckwheat seed-soaking of table and Lead sweet culture statistical form
From Fig. 1~7 as can be seen that germination percentage significantly improves after Gibberellins solution seed soaking；Adventitious root quantity, free amino acid
Content and superoxide dismutase activity reduce to reach close to normal value.Wherein after the gibberellin seed soaking of 40mg/L concentration
Bitter buckwheat growing way and physical signs are closest to CK group, compared to the G0+Pb group germination percentage raising 9% for not adding gibberellin, indefinite radical
Amount reduces 20%, and superoxide dismutase activity reduces 76%, and free aminoacid content reduces 29%.Therefore this experiment obtains alleviation
The gibberellin seed soaking concentration most preferably 40mg/L of Lead sweet.
Although being described in detail in conjunction with attached drawing to a specific embodiment of the invention, should not be construed as special to this
The restriction of the protection scope of benefit.In range described by claims, those skilled in the art are without creative work
The various modifications and deformation that can make still belong to the protection scope of this patent.
Claims (8)
1. a kind of alleviate the method that Lead sweet sprouts bitter buckwheat seed and growth of seedling injures, which is characterized in that including following step
It is rapid:
(1) bitter buckwheat seed of the appearance without scar, full seed is chosen, is dried 2~3 days in the sun, then daily 5~6h is adopted
It is carried out disinfection with thimerosal, finally uses distilled water flushing；
(2) seed after rinsing step (1), which is put into concentration 5~100mg/L Gibberellins solution, to be submerged, in 23~28 DEG C of temperature
Lower constant temperature seed soaking 8~for 24 hours；
(3) seed after step (2) seed soaking is evenly laid out on filter paper, it is placed under aseptic condition, 20~25 DEG C of shading cultures 8
~10 days, watering 2 times, obtained bitter buckwheat seedling daily；
(4) the neat strong sprout of growing way in selecting step (3), plantation to loose smooth soil, root 3~4cm of mulching soil, so
Every plant of seedling applies 0.3~1.2kg mixed fertilizer afterwards.
2. alleviating as described in claim 1, Lead sweet sprouts bitter buckwheat seed and the method for growth of seedling injury, feature exist
In, step (1) when disinfection, use concentration for the disinfection liquid disinfectant 2h of 0.1wt%, then with distilled water flushing 3~5 times.
3. alleviating as claimed in claim 2, Lead sweet sprouts bitter buckwheat seed and the method for growth of seedling injury, feature exist
In the thimerosal is potassium permanganate, formaldehyde or hydrogen peroxide solution.
4. alleviating as described in claim 1, Lead sweet sprouts bitter buckwheat seed and the method for growth of seedling injury, feature exist
In the Gibberellins solution includes following parts by weight of component: 10~15 parts of gibberellin fermented liquid, 80~110 parts of sodium nitrate, sulfuric acid
90~150 parts of ammonium, 90~150 parts of potassium sulfate, 80~110 parts of calcium superphosphate, 15~20 parts of ammonium molybdate and 1100~1400 parts of water.
5. alleviating as claimed in claim 4, Lead sweet sprouts bitter buckwheat seed and the method for growth of seedling injury, feature exist
In the Gibberellins solution includes following parts by weight of component: 13 parts of gibberellin fermented liquid, 90 parts of sodium nitrate, 120 parts of ammonium sulfate,
120 parts of potassium sulfate, 90 parts of calcium superphosphate, 18 parts of ammonium molybdate and 1300 parts of water.
6. alleviating as claimed in claim 5, Lead sweet sprouts bitter buckwheat seed and the method for growth of seedling injury, feature exist
In the gibberellin fermented liquid is prepared by the following method to obtain: gibberellin is fermented 2~3 days under the conditions of 28~30 DEG C, then
It is extracted after concentration, obtains gibberellin fermented liquid.
7. alleviating as described in claim 1, Lead sweet sprouts bitter buckwheat seed and the method for growth of seedling injury, feature exist
In mixed fertilizer described in step (3) is organic fertilizer, urea, potassium oxide and calcium superphosphate by weight 30~60:0.1~0.4:2
The fertilizer that~6:3~7 mix.
8. alleviating as claimed in claim 7, Lead sweet sprouts bitter buckwheat seed and the method for growth of seedling injury, feature exist
In the weight ratio of, organic fertilizer, urea, potassium oxide and calcium superphosphate be 40:0.3:4:5.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207352A (en) * | 2021-05-31 | 2021-08-06 | 云南八凯农业开发有限公司 | Method for improving emergence rate of tartary buckwheat seeds |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207352A (en) * | 2021-05-31 | 2021-08-06 | 云南八凯农业开发有限公司 | Method for improving emergence rate of tartary buckwheat seeds |
| CN113207352B (en) * | 2021-05-31 | 2022-06-17 | 云南八凯农业开发有限公司 | Method for improving emergence rate of tartary buckwheat seeds |
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