CN101707958A - Cutting propagation method of desert caragana - Google Patents
Cutting propagation method of desert caragana Download PDFInfo
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- CN101707958A CN101707958A CN200910071170A CN200910071170A CN101707958A CN 101707958 A CN101707958 A CN 101707958A CN 200910071170 A CN200910071170 A CN 200910071170A CN 200910071170 A CN200910071170 A CN 200910071170A CN 101707958 A CN101707958 A CN 101707958A
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Abstract
The invention relates to a successful cutting propagation method of desert caragana, which comprises the following steps of: selecting a strongly growing bush as a maternal plant, and respectively scissoring a twig, a hard branch and a taproot of the desert caragana for cutting; putting the twig of the desert caragana into 100ppm of growth regulator solution, wherein the soaking depth is 2-3 centimeters, and the soaking time is 6.5-10 hours; putting the hard branch into 500ppm of growth regulator solution, wherein the soaking depth is 2-3 centimeters, and the soaking time is 1-3 hours; soaking the cutting of the taproot in 50ppm of growth regulator solution for 18-20 hours; and selecting a position with flat land and good drainage for arranging a cutting bed, selecting sandy soil as a cutting ground mass, cutting at the depth of 4-5 centimeters, watering after cutting, setting up a shading net in time, watering once every five days, tearing off the shading net after one month, and counting the survival rate after three months. An experiment result indicates that the processing method can ensure the success of the cutting propagation of the desert caragana, and provide abundant germ plasm resources for ecological restoration and stockbreeding production in desert regions.
Description
Technical field
The present invention relates to plant cuttage propagation technique field, particularly a kind of successful methods of desert caragana cottage propagation.
Background technology
Kind surplus Caragana (Caragana) whole world has 100 is machaka, is celebrated with its strong drought resistant.Be described as the JIUMINGCAO of pastoral area domestic animal.Distribution and fauna constituent analysis about population pattern change studies show that Caragana mainly is distributed in grassland and desert district.Desert caragana (C.roborovskyi) belongs to the desert kind, is distributed in the capable and experienced non-irrigated desert and the extreme drought desert in NORTHWEST CHINA area, is the important ecological shrub in desert district, also is the important feed species that livestock breeding is produced.In today that country pays much attention to the ecological construction in west area, the quick breeding of research population pattern change kind not only can be enriched the ecological research of grassland and deserta, more can provide more feed species for the production of livestock breeding.Under field conditions (factors), owing to be subjected to arid and animal ingestion dual-pressure, desert caragana seed is difficult to ripe, even indivedual mature seeds are arranged, also is difficult to sprouting, growth under harsh environment, so cannot see desert caragana seedling substantially.In the desert district, the desert caragana mainly increases the plant division tree by clonal growth, and the population expansion rate is very slow.Cottage propagation is a regeneration capacity of utilizing the nutrition organs of plant, becomes the independently method of new plant in artificial auxiliary breeding down.A lot of plants can carry out cottage propagation, increase population quantity fast, but the method for desert caragana cottage propagation are not seen bibliographical information as yet.
Summary of the invention
The object of the present invention is to provide a kind of successful methods of desert caragana cottage propagation.In order to prove the feasibility of desert caragana cottage propagation, explore its cuttage technique, realize the quick increase of desert caragana population, the inventor has carried out desert caragana cuttage experimental study.Experimental result proves: desert caragana cottage propagation of the present invention is feasible.Particularly adopt ABT1 to handle and effectively to improve desert caragana plugged ear survival rate.Test method of the present invention is as follows:
The method of desert caragana cottage propagation is characterized in that being undertaken by following step:
(1) select robust growth, germinate bar is many and amount of growth is big individuality as maternal plant then, clip desert caragana spray, hard branch and main root are used for cuttage respectively; Wherein:
1) desert caragana spray is cut into the long plugged ear of 7～12cm, removes meticulous or tenderer scolus, the upper end keeps 2～3 impeller blades, and is bundled by 25 strains bundle; The plugged ear that bundle is good is put into 100ppm growth regulating agent solution, and soaking depth is 2～3cm, soaks 6.5～10h;
2) the hard lopwood bar of desert caragana is cut into the plugged ear of growing 12～15cm, removes meticulous end of the branch, bud 0.5～0.8cm place is cut into the shape of a hoof under the following end distance, and is bundled by 25 strains bundle; The plugged ear that bundle is good is put into 500ppm growth regulating agent solution, and soaking depth is 2～3cm, and soak time is 1～3h;
3) be that the main root of 0.5～0.8cm is cut into long 8.0～10cm with desert caragana diameter, the cutting of flat declivity on the end face, bundled by 25 strains bundle; The plugged ear that bundle is good is put into 50ppm growth regulating agent solution, and soaking depth is 3～4cm, and soak time is 18～20h;
(2) cutting bed and matrix:
Cutting bed selection physical features is smooth, draining is well located to be provided with, the wide 1.0m of bed surface, and cutting medium is selected sandy soil, and the good bed surface of cuttage prefinishing is irritated permeable, and is used 0.5% disinfecting solution of potassium permanganate; Take the arch form shading screen on the slotting machine;
(3) cottage method and density:
Prick slotting cave at bed surface by 2 * 5cm density with leading cone, afterwards treated plugged ear is directly inserted in the hole, cave, the cuttage degree of depth 4～5cm inserts suppression hole, back, and plugged ear is fully contacted with matrix, avoids the fringe bar unsettled;
(4) insert the back management:
Water after inserting, in time set up shading screen, watered water one time in 5 days, throw off shading screen after one month, add up survival rate after three months.
The cuttage breeding method of desert caragana of the present invention, growth regulator wherein can be one or more the mixtures of indolebutyric acid, heteroauxin, root-inducing powder ABT1#, root-inducing powder ABT2#.The mixture formed of indolebutyric acid and root-inducing powder ABT1# for example; Mixture that heteroauxin and root-inducing powder ABT2# form or the like.The preferred growth regulator of the present invention is ABT1# or ABT2# root-inducing powder.Another preferred growth regulator of the present invention is 50ppm-500ppm ABT1# or ABT2# root-inducing powder.
In preferred embodiment of the present invention, desert caragana spray need be put into 100ppm ABT1# rooting powder solution, and soaking depth is 2～3cm, soaks 6.5～10h;
In another preferred embodiment of the present invention, the hard lopwood bar of desert caragana need be put into 500ppm ABT1# rooting powder solution, and soaking depth is 2～3cm, and soak time is 1～3h;
In another preferred embodiment of the present invention, diameter is ABT1 immersion 18～20h that the main root of 0.5～0.8cm desert caragana need be put into 50ppm.
In another preferred embodiment of the present invention, cutting medium is a sandy soil.
More detailed test method of the present invention and step thereof are as follows:
1 test site and material
1.1 test site
Test is carried out Alashan, district, capable and experienced nonirrigated farmland in Inner Mongolia Autonomous Region.Test site is positioned at 105.66 ° of east longitudes, 38.84 ° of north latitude, height above sea level 1561.0m, mean annual precipitation is 110mm, year-round average temperature is 7.80 ℃, 〉=10 ℃ year 3250 ℃ of accumulated temperature, the sunshine time is 3200h, total radiation 625.0kJcm
-2, soil moisture content 1.73% (30cm, 1.66%; 60cm, 1.55%; 100cm, 1.98%); So that dust storm is big, arid is short of rain, sunshine is sufficient, evaporation is main feature strongly; It is the natural distributed district of desert caragana.
1.2 examination material source
Tested in July, 2009 and carry out, select robust growth, growing way obviously to be better than other plant, to germinate bar is many and amount of growth is big desert caragana shrubbery as maternal plant then.Clip on the basic strain main root of opening from excavation for the main root of examination, and clip spray and hard branch respectively simultaneously are used for cuttage.
2 test methods
2.1 plugged ear and processing
Spray is cut into the long plugged ear of 7～12cm, removes scolus, the upper end keeps 2～3 impeller blades.Cross withe or tenderer tip of a branch rejecting, bundled by 25 strains bundle.Plugged ear is handled with 100ppm ABT1 rooting powder solution, and soaking depth is 2～3cm, soaks 6.5h.6.5h is treated to contrast with water logging.
The hard lopwood bar of adopting is cut into the plugged ear of growing 12～15cm, removes meticulous end of the branch, bud 0.5～0.8cm place is cut into the shape of a hoof under the following end distance, and 1 bundle is tied in the per 25 strain base portions alignment of the plugged ear that shears.The plugged ear that bundle is good is put into 500ppm ABT1 rooting powder solution and clear water (contrast) respectively, and soaking depth is 2～3cm, and soak time is 1h.
With diameter is that the main root of 0.5～0.8cm is cut into and is about 8.0cm, the cutting of flat declivity on the end face.ABT1 rooting powder solution with 50ppm soaks cutting base portion (3-4cm height) 18h, and compares with clear water.
2.2 cutting bed and matrix
Cutting bed selection physical features is smooth, draining is well located to be provided with, the wide 1.0m of bed surface, and cutting medium is selected sandy soil.The good bed surface of cuttage prefinishing is irritated permeable, and is used 0.5% disinfecting solution of potassium permanganate.Take on the slotting machine arch form shading screen avoid illumination too strong, evaporate by force and influenced survival rate, play moisture-keeping function simultaneously.
2.3 cottage method and density
Rubbing with the hands for the time damage fringe skin that prevents cuttage or the slurries of band medicine influences rooting efficiency, uses leading cone to prick by 2 * 5cm density at bed surface before inserting and inserts the cave, afterwards treated plugged ear is directly inserted in the hole, cave the cuttage degree of depth 4～5cm.Insert the back and suppress the hole, plugged ear is fully contacted with matrix, avoid the fringe bar unsettled with hand.
2.4 insert the back management
Water after inserting, in time set up shading screen, watered a water (caragana is an xerophilous tree species, and the excessive fringe dermatophyte that easily causes of soil humidity is mashed) in 5 days.Throw off shading screen after one month, add up survival rate after three months.
3 results
Table 1 desert caragana plugged ear survival rate statistics
Under the sandy matrix, it is 14% that spray plugged ear ABT1 is processed into motility rate, and contrasts 6%; It is 4% that hard branch ABT1 is processed into motility rate, and contrast is 2.0%; It is 4% that root plugged ear ABT1 is processed into motility rate, contrast 2% (table 1).
4 conclusions:
Desert caragana cottage propagation is feasible.Desert caragana plugged ear survival rate spray is greater than hard branch and root, and this draws materials to cuttage is favourable, because sprout is more, the Shi Buhui that draws materials brings big injury to maternal plant.ABT1 handles and can effectively improve desert caragana plugged ear survival rate simultaneously.For desert district ecological recovery and livestock breeding production provide abundant germ plasm resource.
Embodiment
For simple and purpose clearly, hereinafter appropriate omission the description of known technology, in order to avoid those unnecessary details influences are to the description of the technical program.The present invention is described further below in conjunction with test examples.Be several specific embodiments below, in all these examples, used water is distilled water.
Embodiment 1.
(1) select robust growth, growing way obviously to be better than other plant, to germinate bar is many and amount of growth is big desert caragana shrubbery as maternal plant then.Main root clip on the basic strain main root of opening from excavation on July 10th, 2009 of confession examination is taken back in order to cuttage and is used.In morning July 11, clip desert caragana spray and hard branch are used for cuttage respectively.
(2) spray is cut into the long plugged ear of 7cm, removes scolus, the upper end keeps 2 impeller blades.Cross withe or tenderer tip of a branch rejecting, bundled by 25 strains bundle.Plugged ear is handled with 100ppm ABT1 rooting powder solution, and soaking depth is 2cm, soaks 6.5h.6.5h is treated to contrast with water logging.
The hard lopwood bar of adopting is cut into the plugged ear of long 12cm, removes meticulous end of the branch, bud 0.5cm place is cut into the shape of a hoof under the following end distance, and 1 bundle is tied in the per 25 strain base portions alignment of the plugged ear that shears.The plugged ear that bundle is good is put into 500ppm ABT1 rooting powder solution and clear water (contrast) respectively, and soaking depth is 2cm, and soak time is 1h.
With desert caragana diameter is that the main root of 0.5cm is cut into and is about 8.0cm, the cutting of flat declivity on the end face.ABT1 rooting powder solution with 50ppm soaks cutting base portion (about 3cm height) 18h, and compares with clear water.
(3) cutting bed selects that physical features is smooth, draining is well located to be provided with, the wide 1.0m of bed surface, and cutting medium is selected sandy soil.The good bed surface of cuttage prefinishing is irritated permeable, and is used 0.5% disinfecting solution of potassium permanganate.Take on the slotting machine arch form shading screen avoid illumination too strong, evaporate by force and influenced survival rate, play moisture-keeping function simultaneously.
(4) rub with the hands for the time damage fringe skin that prevents cuttage or the slurries of band medicine and influence rooting efficiency, use leading cone to prick by 2 * 5cm density before inserting and insert the cave, afterwards treated plugged ear is directly inserted in the hole, cave cuttage degree of depth 5cm at bed surface.Insert the back and suppress the hole, plugged ear is fully contacted with matrix, avoid the fringe bar unsettled with hand.
(5) water after inserting, in time set up shading screen, watered a water (caragana is an xerophilous tree species, and the excessive fringe dermatophyte that easily causes of soil humidity is mashed) in 5 days.Throw off shading screen after one month, add up survival rate after three months.
Table 2 desert caragana plugged ear survival rate statistics
Embodiment 2
(1) select robust growth, growing way obviously to be better than other plant, to germinate bar is many and amount of growth is big desert caragana shrubbery as maternal plant then.Main root clip on the basic strain main root of opening from excavation on July 13rd, 2009 of confession examination is taken back in order to cuttage and is used.In morning July 14, clip desert caragana spray and hard branch are used for cuttage respectively.
(2) spray is cut into the long plugged ear of 12cm, removes scolus, the upper end keeps 3 impeller blades.Cross withe or tenderer tip of a branch rejecting, bundled by 25 strains bundle.Plugged ear is handled with 100ppm ABT1 rooting powder solution, and soaking depth is 3cm, soaks 6.5h.6.5h is treated to contrast with water logging.
The hard lopwood bar of adopting is cut into the plugged ear of growing 12～15cm, removes meticulous end of the branch, bud 0.5～0.8cm place is cut into the shape of a hoof under the following end distance, and 1 bundle is tied in the per 25 strain base portions alignment of the plugged ear that shears.The plugged ear that bundle is good is put into 500ppm ABT1 rooting powder solution and clear water (contrast) respectively, and soaking depth is 3cm, and soak time is 1h.
With desert caragana diameter is that the main root of 0.8cm is cut into and is about 9cm, the cutting of flat declivity on the end face.ABT1 rooting powder solution with 50ppm soaks cutting base portion (about 4cm height) 18h, and compares with clear water.
(3) cutting bed selects that physical features is smooth, draining is well located to be provided with, the wide 1.0m of bed surface, and cutting medium is selected sandy soil.The good bed surface of cuttage prefinishing is irritated permeable, and is used 0.5% disinfecting solution of potassium permanganate.Take on the slotting machine arch form shading screen avoid illumination too strong, evaporate by force and influenced survival rate, play moisture-keeping function simultaneously.
(4) rub with the hands for the time damage fringe skin that prevents cuttage or the slurries of band medicine and influence rooting efficiency, use leading cone to prick by 2 * 5cm density before inserting and insert the cave, afterwards treated plugged ear is directly inserted in the hole, cave the cuttage degree of depth 4～5cm at bed surface.Insert the back and suppress the hole, plugged ear is fully contacted with matrix, avoid the fringe bar unsettled with hand.
(5) water after inserting, in time set up shading screen, watered a water (caragana is an xerophilous tree species, and the excessive fringe dermatophyte that easily causes of soil humidity is mashed) in 5 days.Throw off shading screen after one month, add up survival rate after three months.
Table 3 desert caragana plugged ear survival rate statistics
After the preferred embodiment that describes in detail, being familiar with this technology personage can be well understood to, can carry out various variations and modification not breaking away under above-mentioned claim and the spirit, all foundations technical spirit of the present invention is done any simple modification, equivalent variations and modification to above embodiment, all belongs to the scope of technical solution of the present invention.And the present invention also is not subjected to the restriction of the embodiment that gives an actual example in the specification.
Claims (3)
1. the cuttage breeding method of desert caragana is characterized in that being undertaken by following step:
(1) select robust growth, germinate bar is many and amount of growth is big individuality as maternal plant then, clip desert caragana spray, hard branch and main root are used for cuttage respectively; Wherein:
1) desert caragana spray is cut into the long plugged ear of 7～12cm, removes meticulous or tenderer scolus, the upper end keeps 2～3 impeller blades, and is bundled by 25 strains bundle; The plugged ear that bundle is good is put into 100ppm growth regulating agent solution, and soaking depth is 2～3cm, soaks 6.5～10h;
2) the hard lopwood bar of desert caragana is cut into the plugged ear of growing 12～15cm, removes meticulous end of the branch, bud 0.5～0.8cm place is cut into the shape of a hoof under the following end distance, and is bundled by 25 strains bundle; The plugged ear that bundle is good is put into 500ppm growth regulating agent solution, and soaking depth is 2～3cm, and soak time is 1～3h;
3) be that the main root of 0.5～0.8cm is cut into long 8.0～10cm with desert caragana diameter, the cutting of flat declivity on the end face, bundled by 25 strains bundle; The plugged ear that bundle is good is put into 50ppm growth regulating agent solution, and soaking depth is 3～4cm, and soak time is 18～20h;
(2) cutting bed and matrix:
Cutting bed selection physical features is smooth, draining is well located to be provided with, the wide 1.0m of bed surface, and cutting medium is selected sandy soil, and the good bed surface of cuttage prefinishing is irritated permeable, and is used 0.5% disinfecting solution of potassium permanganate; Take the arch form shading screen on the slotting machine;
(3) cottage method and density:
Prick slotting cave at bed surface by 2 * 5cm density with leading cone, afterwards treated plugged ear is directly inserted in the hole, cave, the cuttage degree of depth 4～5cm inserts suppression hole, back, and plugged ear is fully contacted with matrix, avoids the fringe bar unsettled;
(4) insert the back management:
Water after inserting, in time set up shading screen, watered water one time in 5 days, throw off shading screen after one month, add up survival rate after three months.
2. the cuttage breeding method of the described desert caragana of claim 1, growth regulator wherein are one or more the mixture of indolebutyric acid, heteroauxin, root-inducing powder ABT1#, root-inducing powder ABT2#.
3. the method for the described desert caragana of claim 1 cottage propagation, wherein said cutting time is summer.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228002A (en) * | 2011-04-28 | 2011-11-02 | 东北林业大学 | Micropropagation method of caragana fruticosa |
| CN103460965A (en) * | 2013-09-27 | 2013-12-25 | 中国科学院新疆生态与地理研究所 | Method for growing seedlings of medicinal plant caragana rosea |
| CN105145579A (en) * | 2015-10-10 | 2015-12-16 | 北京阿格瑞斯生物技术有限公司 | Composite cutting rooting agent for caragana as well as preparation method and application of agent |
| CN105684845A (en) * | 2016-01-18 | 2016-06-22 | 湖北民族学院 | Caraganasinica(Buchoz)Rehd. cuttage breeding method |
| CN107372075A (en) * | 2017-09-14 | 2017-11-24 | 河南科技学院 | A kind of cuttage breeding method of chicken leg violet |
| CN111034530A (en) * | 2019-12-30 | 2020-04-21 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for planting caragana in sand |
-
2009
- 2009-11-05 CN CN200910071170A patent/CN101707958A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228002A (en) * | 2011-04-28 | 2011-11-02 | 东北林业大学 | Micropropagation method of caragana fruticosa |
| CN102228002B (en) * | 2011-04-28 | 2013-01-02 | 东北林业大学 | Micropropagation method of caragana fruticosa |
| CN103460965A (en) * | 2013-09-27 | 2013-12-25 | 中国科学院新疆生态与地理研究所 | Method for growing seedlings of medicinal plant caragana rosea |
| CN105145579A (en) * | 2015-10-10 | 2015-12-16 | 北京阿格瑞斯生物技术有限公司 | Composite cutting rooting agent for caragana as well as preparation method and application of agent |
| CN105145579B (en) * | 2015-10-10 | 2018-03-16 | 北京阿格瑞斯生物技术有限公司 | Compound cuttage root-taking agent of caragana and its preparation method and application |
| CN105684845A (en) * | 2016-01-18 | 2016-06-22 | 湖北民族学院 | Caraganasinica(Buchoz)Rehd. cuttage breeding method |
| CN107372075A (en) * | 2017-09-14 | 2017-11-24 | 河南科技学院 | A kind of cuttage breeding method of chicken leg violet |
| CN111034530A (en) * | 2019-12-30 | 2020-04-21 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for planting caragana in sand |
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Open date: 20100519 |