CN103404443B - Method for culturing seedlings by cutting leaves of manglietia insignis - Google Patents
Method for culturing seedlings by cutting leaves of manglietia insignis Download PDFInfo
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- CN103404443B CN103404443B CN201310373664.8A CN201310373664A CN103404443B CN 103404443 B CN103404443 B CN 103404443B CN 201310373664 A CN201310373664 A CN 201310373664A CN 103404443 B CN103404443 B CN 103404443B
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Abstract
The invention discloses a method for culturing seedlings by cutting leaves of manglietia insignis. The method comprises the steps of selecting manglietia insignis plants which grow strongly and are not damaged by diseases and pests; shearing currently grown lignified branches, taking leaves from the branches down together with phloems, quickly dipping petiole ends with the phloems in rooting liquid for 10s-15s, inserting the leaves in a culture medium for 2cm-3cm together with the petioles, pressing the culture medium, watering and covering a film on a small shed cover, keeping the humidity of air in a small shed to be 80-90 percent, spraying the rooting liquid once after 15 days after insertion, spraying a sterilizing solution once every 5-7 days by combining with watering, and spraying 0.05-0.1 percent urea and a 0.01-0.05 percent monopotassium phosphate water solution once every 10-15 days. After 25-30 days, root primordial are differentiated from callus, and radicles grow; buds are differentiated and grow from the petioles and axillae after 40-50 days. According to the invention, the growth and the tree form of original trees are influenced a little when the materials are taken; the method is simple to operate, low in cost and easy to popularize, and brings convenience for mass propagation of manglietia insignis.
Description
Art
The invention belongs to seedling propagation technical field, particularly relate to a kind of method of Fructus Manglietiae insignis blade cuttage and seedling culture.
Background technology
Fructus Manglietiae insignis is evergreen broad-leaved arbor in Magnoliaceae Manglietia, tree-like grace, and the four seasons are evergreen, and its leaf is dark green, delicate, keratin, single leaf alternate, in oval shape ellipse, Long Circle or the lanceolar that falls, tree-like grace in great numbers, spend beautiful greatly, the gorgeous fragrance of pattern is desirable garden ornamental tree species.Trunk is logical straight, wooden excellent, and bark and branch skin also have good medical value, are again one of rare and endangered tree species that economic worth is very high.Fructus Manglietiae insignis is more original kind, is listed in the famous and precious rare or endangered species of national second class protection.
The natural reproduction year Age of Fructus Manglietiae insignis generally needs generation seed of just yielding positive results for 5 years, and due to the impact by factors such as heredity, physiology and environment, naturally solid generation seed is little, attracts birds institute very few after maturation.Have now report to be that propagating materials carries out cuttage and seedling culture to Fructus Manglietiae insignis with branch, generally all will retain 1-2 axillalry bud when clip cutting, the branch of needs is many, meanwhile, has impact when clip branch to the tree-like of elite stand and growth.Therefore, be usually subject to the restriction of propagating materials and affect amount reproduction, the protection and developmental utilization of people to Fructus Manglietiae insignis plant.
Summary of the invention
The object of this invention is to provide a kind of method that Fructus Manglietiae insignis leaf inserts nursery, changing with branch is the defect that propagating materials is large to Fructus Manglietiae insignis damage, breeding amount is low, to solve the deficiencies in the prior art.
A kind of Fructus Manglietiae insignis leaf inserts the method for nursery, the sunny day of mid or late August to mid-September, select robust growth without the Fructus Manglietiae insignis plant of damage by disease and insect, clip is raw lignification branch then, semi-lignified on branch or lignified blade, from branch, blade is taken off by related phloem in the lump, in taking root liquid, 10-15 second is dipped in soon to be with the petiole end of phloem, in base, press 10*10 centimetre distance with little bamboo chip and make a call to a dark 2-3 centimetre sealing, again the blade dipping in taking root liquid is inserted in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, blade for cuttage can not have any scar.Water after having inserted and take Small plastic shed and cover film, and reality that the surrounding of film is obturaged, before blade is taken root, in Small plastic shed, air humidity remains on 80-90%, illumination will take sunshade net by force, insert and spray a taking root liquid again in latter 15 days, a bactericidal liquid is sprayed in conjunction with the every 5-7 days that waters, every 10-15 days sprays a 0.05-0.1% urea and 0.01-0.05% potassium dihydrogen phosphate aqueous solution, within about 20 days, petiole base phloem starts to break up callus, within 25-30 days, Calli Differentiation goes out root restriction and grows young root, within 40-50 days, start to put out new shoots from petiole and axil punishmentization, cultivated through 60-70 days again and just can carry out transplant planting.
Described medium is perlite, the peat composed of rotten mosses, mixes be mixed with medium in 1:1 ratio.Within 7-10 days before inserting, with 0.5% potassium permanganate solution, medium is carried out disinfection sterilization, cover plastic film, open film after 5-7 days for subsequent use.
Described taking root liquid formula is gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, by ratio of weight and the number of copies 0.15:0.2:0.005:0.5:0.1:0.1, and all the other compositions are sterile water, and working concentration is 800-1000 ㎎/L.
Medium material of the present invention, the more careless loose permeability of perlite particle is good, and water suction and the moisture capacity of the peat composed of rotten mosses are relatively good, and cuttage medium is made in both mixing, not only ventilative but also water conservation, the respiration of favourable cuttage material, thus hestening rooting.
Potassium permanganate solution carries out sterilizing to seedling bed earth, the multiple diseases of control flowers and trees, and to person poultry safety, not easily produces poisoning to flowers and trees, and noresidue is pollution-free, and meanwhile, potassium permanganate is also containing potassium, manganese two plant development institute indispensable element.
Gibberellin: have and promote cell division, elongation, leaf expansion, stem extends, collateral generation, breaking dormancy, stratification, and the physiological function such as delay senility.
6-benzyl aminopurine: belong to Cytokinin Substances, absorbs through root, stem, blade, enters in body, promote cell division, the differentiation of undifferentiated tissue, cell is increased, promotes seed sprouting, induced dormancy blastogenesis is long, promote that stem, leaf are raw and extend life, the growth of root, breaks apical dominance, promote lateral bud growth, suppress chlorophyll to decompose, suppress blade aging, protect green and delay blade flavescence ageing.
Ascorbic acid: participate in redox in electron transport system in plant corpus, promote plant metabolism, to the aldehydes matter Wheat Protein that cuttage material produces, the aldehydes matter oozed out during blade cuts can be suppressed to cause autointoxication brown stain, leaf growth is paused, loses differentiation capability.
Glycine: the constituent being protein, can provide organic nitrogen source, plays a driving role to the differentiation of the growth of cuttage blade and indefinite bud, adventive root.
Glucose: the main source being living cells institute energy requirement, supplies heat while being oxidized to carbonic acid gas and water in vivo, is indispensable material in vital movement, can participates in metabolic processes directly, the formation of favourable promotion adventive root.
Fulvic acid: can be absorbed by plant leaf blade, improves respiration, increases chlorophyll content, promotes photosynthesis.Reduce Stoma of Leaves opening degree, reduce plant leaf blade rising, reduce moisture loss, promote plant establishment and Nutrient Absorption.
Due to the photosynthesis of cuttage blade self, can the material being conducive to taking root be synthesized, have facilitation to blade cuttage root-taking.Blade cuttage and seedling culture compares saves cuttage material, is 3-5 times of branch cutting to the utilization of cuttage material.Implement the present invention, when drawing materials on the growth of elite tree wood and tree-like impact less, simple to operate, cost is low, easily popularizes, and is convenient to carry out amount reproduction to Fructus Manglietiae insignis.
Embodiment
Below in conjunction with example, the present invention is described further:
Embodiment 1
A kind of Fructus Manglietiae insignis leaf inserts the method for nursery: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.In insert before 7-10 days with 0.5% potassium permanganate solution process medium after, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, by ratio of weight and the number of copies 0.15:0.2:0.005:0.5:0.1:0.1, all the other compositions are sterile water, and concentration is 1000 ㎎/L.
3, blade is chosen and cuttage: mid-August sunny day, select robust growth without the Fructus Manglietiae insignis plant of damage by disease and insect, clip is raw lignification branch then, semi-lignified on branch or lignified blade, from branch, blade is taken off by related phloem in the lump, in taking root liquid, 15 seconds are dipped in soon to be with the petiole end of phloem, in base, making a call to a degree of depth with little bamboo chip by 10*10 centimetre distance is 2-3 centimetre sealing, again the blade dipping in taking root liquid is inserted in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, the blade for cuttage can not have any scar.Water after often having inserted one case seedbed and take Small plastic shed and cover film, and reality that the surrounding of film is obturaged.
4, manage after cuttage: before blade is taken root, in Small plastic shed, air humidity remains on 80-90%, and to spray blade with clear water if desired, medium protects special moisture state, will take sunshade net when illumination is strong.Insert and spray taking root liquid, hestening rooting and a germination again in latter 15 days.Spray a bactericidal liquid in conjunction with the every 5-7 days that waters, every 10-15 days sprays a 0.05-0.1% urea and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Within about 20 days, petiole base phloem starts to break up callus, and within 25-30 days, Calli Differentiation goes out root restriction and grows young root, within 40-50 days, starts to put out new shoots from petiole and axil punishmentization, more just can carry out transplant planting through cultivation in 60-70 days.
Embodiment 2
A kind of Fructus Manglietiae insignis leaf inserts the method for nursery: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.In insert before 7-10 days with 0.5% potassium permanganate solution process medium after, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, by ratio of weight and the number of copies 0.15:0.2:0.005:0.5:0.1:0.1, all the other compositions are sterile water, and concentration is 900 ㎎/L.
3, blade is chosen and cuttage: late August sunny day, select robust growth without the Fructus Manglietiae insignis plant of damage by disease and insect, clip is raw lignification branch then, semi-lignified on branch or lignified blade, from branch, blade is taken off by related phloem in the lump, in taking root liquid, 12 seconds are dipped in soon to be with the petiole end of phloem, in base, press 10*10 centimetre distance with little bamboo chip and make a call to a sealing, again the blade dipping in taking root liquid is inserted in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, the blade for cuttage can not have any scar.Water after often having inserted one case seedbed and take Small plastic shed and cover film, and reality that the surrounding of film is obturaged.
4, manage after cuttage: before blade is taken root, in Small plastic shed, air humidity remains on 80-90%, and to spray blade with clear water when humidity does not reach, medium protects special moisture state, will take sunshade net when illumination is strong.Insert and spray taking root liquid, hestening rooting and a germination again in latter 15 days.Spray a bactericidal liquid in conjunction with the every 5-7 days that waters, every 10-15 days sprays a 0.05-0.1% urea and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Within about 25 days, petiole base phloem starts to break up callus, and within about 30 days, Calli Differentiation goes out root restriction and grows young root, within 40-50 days, starts to put out new shoots from petiole and axil punishmentization, more just can carry out transplant planting through cultivation in 60-70 days.
Embodiment 3
A kind of Fructus Manglietiae insignis leaf inserts the method for nursery: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.In insert before 7-10 days with 0.5% potassium permanganate solution process medium after, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, by ratio of weight and the number of copies 0.15:0.2:0.005:0.5:0.1:0.1, all the other compositions are sterile water, and concentration is 1000 ㎎/L.
3, blade is chosen and cuttage: early September sunny day, select robust growth without the Fructus Manglietiae insignis plant of damage by disease and insect, clip is raw lignification branch then, semi-lignified on branch or lignified blade, from branch, blade is taken off by related phloem in the lump, in taking root liquid, 10 seconds are dipped in soon to be with the petiole end of phloem, in base, press 10*10 centimetre distance with little bamboo chip and make a call to a sealing, again the blade dipping in taking root liquid is inserted in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, the blade for cuttage can not have any scar.Water after often having inserted one case seedbed and take Small plastic shed and cover film, and reality that the surrounding of film is obturaged.
4, manage after cuttage: before blade is taken root, in Small plastic shed, air humidity remains on 80-90%, and to spray blade with clear water when humidity does not reach, medium protects special moisture state, will take sunshade net when illumination is strong.Insert and spray taking root liquid, hestening rooting and a germination again in latter 15 days.Spray a bactericidal liquid in conjunction with the every 5-7 days that waters, every 10-15 days sprays a 0.05-0.1% urea and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Within about 25 days, petiole base phloem starts to break up callus, and within 30-35 days, Calli Differentiation goes out root restriction and grows young root, within 40-50 days, starts to put out new shoots from petiole and axil punishmentization, more just can carry out transplant planting through cultivation in 60-70 days.
Embodiment 4
A kind of Fructus Manglietiae insignis leaf inserts the method for nursery: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.In insert before 7-10 days with 0.5% potassium permanganate solution process medium after, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, by ratio of weight and the number of copies 0.15:0.2:0.005:0.5:0.1:0.1, all the other compositions are sterile water, and concentration is 800 ㎎/L.
3, blade is chosen and cuttage: mid-September sunny day, select robust growth without the Fructus Manglietiae insignis plant of damage by disease and insect, clip is raw lignification branch then, semi-lignified on branch or lignified blade, from branch, blade is taken off by related phloem in the lump, in taking root liquid, 15 seconds are dipped in soon to be with the petiole end of phloem, in base, press 10*10 centimetre distance with little bamboo chip and make a call to a sealing, again the blade dipping in taking root liquid is inserted in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, the blade for cuttage can not have any scar.Water after often having inserted one case seedbed and take Small plastic shed and cover film, and reality that the surrounding of film is obturaged.
4, manage after cuttage: before blade is taken root, in Small plastic shed, air humidity remains on 80-90%, and to spray blade with clear water when humidity does not reach, medium protects special moisture state, will take sunshade net when illumination is strong.Insert and spray taking root liquid, hestening rooting and a germination again in latter 15 days.Spray a bactericidal liquid in conjunction with the every 5-7 days that waters, every 10-15 days sprays a 0.05-0.1% urea and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Within about 30-35 days, petiole base phloem starts to break up callus, and within 40-45 days, Calli Differentiation goes out root restriction and grows young root, within 40-50 days, starts to put out new shoots from petiole and axil punishmentization, more just can carry out transplant planting through cultivation in 60-70 days.
Practice result shows: produce Calli Differentiation after the taking root liquid of several concentration all can make blade cuttage, average rooting rate reaches 73.6%, is up to 86%.Concentration of taking root is best with the effect of 15 seconds with mid-August, processing time with 1000 ㎎/L, cutting time.
Blade cutting experiment statistical form
Claims (1)
1. a Fructus Manglietiae insignis leaf inserts the method for nursery, it is characterized in that: the fine day of mid or late August to mid-September, select robust growth without the Fructus Manglietiae insignis plant of damage by disease and insect, clip is raw lignification branch then, semi-lignified on branch or lignified blade, from branch, blade is taken off by related phloem in the lump, in taking root liquid, 10-15 second is dipped in soon to be with the petiole end of phloem, taking root liquid formula is gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, 0.15:0.2:0.005:0.5:0.1:0.1 by ratio of weight and the number of copies, working concentration is 800-1000 ㎎/L, all the other compositions are sterile water, a dark 2-3 centimetre sealing is made a call to by 10 × 10 centimeters of distances in the medium with little bamboo chip, again the blade dipping in taking root liquid is inserted in medium, medium is perlite, the peat composed of rotten mosses, mix formulated in 1:1 ratio, cultivate based on inserting first 7-10 days with after 0.5% potassium permanganate solution disinfection, cover plastic film, film is opened after 5-7 days, insertion depth is together with petiole 2-3 centimetre, compress medium, blade for cuttage can not have any scar, water after having inserted and take Small plastic shed and cover film, and reality that the surrounding of film is obturaged, before blade is taken root, in Small plastic shed, air humidity remains on 80-90%, illumination will take sunshade net by force, insert and spray a taking root liquid again in latter 15 days, a bactericidal liquid is sprayed in conjunction with the every 5-7 days that waters, every 10-15 days sprays a 0.05-0.1% urea and 0.01-0.05% potassium dihydrogen phosphate aqueous solution, within about 20 days, petiole base phloem starts to break up callus, within 25-30 days, Calli Differentiation goes out root restriction and grows young root, within 40-50 days, start to put out new shoots from petiole and axil punishmentization, cultivated through 60-70 days again and carry out transplant planting.
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Families Citing this family (7)
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| ES2787042T3 (en) * | 2014-07-11 | 2020-10-14 | Medicago Inc | Modification of protein production in plants |
| CN104813939B (en) * | 2015-02-05 | 2017-01-25 | 南京农业大学 | Method for constructing lotus regeneration system |
| CN104641921B (en) * | 2015-03-20 | 2016-08-17 | 日照恒久丰农业科技有限公司 | The blade cuttage breeding method of somewhat-white magnolia |
| CN105432280A (en) * | 2015-11-26 | 2016-03-30 | 全椒县桃花湾果蔬种植专业合作社 | Cultivation method for potting glechoma hedracea |
| CN105409780B (en) * | 2015-12-30 | 2019-03-29 | 玉溪市飞熊农业开发有限公司 | A kind of plant tissue culture culture medium and preparation method thereof without sterilizing |
| CN106034591B (en) * | 2016-05-26 | 2019-04-05 | 宜宾云辰乔木园林有限责任公司 | A method of Fructus Manglietiae insignis is bloomed ahead of time |
| CN112544444B (en) * | 2020-12-03 | 2022-03-22 | 中国科学院昆明植物研究所 | Tissue culture medium for manglietia insignis, method for culturing embryonic callus of manglietia insignis and method for rapidly propagating manglietia insignis |
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| CN101297625A (en) * | 2008-06-17 | 2008-11-05 | 江苏省林业科学研究院 | Cottage method of Photinia glabra leaves |
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| CN101297625A (en) * | 2008-06-17 | 2008-11-05 | 江苏省林业科学研究院 | Cottage method of Photinia glabra leaves |
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| 园林植物生根剂一览;岳建国;《山西农业》;20050601;第53-54页 * |
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