CN103404443A - Method for culturing seedlings by cutting leaves of manglietia insignis - Google Patents
Method for culturing seedlings by cutting leaves of manglietia insignis Download PDFInfo
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- CN103404443A CN103404443A CN2013103736648A CN201310373664A CN103404443A CN 103404443 A CN103404443 A CN 103404443A CN 2013103736648 A CN2013103736648 A CN 2013103736648A CN 201310373664 A CN201310373664 A CN 201310373664A CN 103404443 A CN103404443 A CN 103404443A
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Abstract
The invention discloses a method for culturing seedlings by cutting leaves of manglietia insignis. The method comprises the steps of selecting manglietia insignis plants which grow strongly and are not damaged by diseases and pests; shearing currently grown lignified branches, taking leaves from the branches down together with phloems, quickly dipping petiole ends with the phloems in rooting liquid for 10s-15s, inserting the leaves in a culture medium for 2cm-3cm together with the petioles, pressing the culture medium, watering and covering a film on a small shed cover, keeping the humidity of air in a small shed to be 80-90 percent, spraying the rooting liquid once after 15 days after insertion, spraying a sterilizing solution once every 5-7 days by combining with watering, and spraying 0.05-0.1 percent urea and a 0.01-0.05 percent monopotassium phosphate water solution once every 10-15 days. After 25-30 days, root primordial are differentiated from callus, and radicles grow; buds are differentiated and grow from the petioles and axillae after 40-50 days. According to the invention, the growth and the tree form of original trees are influenced a little when the materials are taken; the method is simple to operate, low in cost and easy to popularize, and brings convenience for mass propagation of manglietia insignis.
Description
Affiliated technical field
The invention belongs to the seedling propagation technical field, relate in particular to a kind of method of Fructus Manglietiae insignis blade cuttage and seedling culture.
Background technology
Fructus Manglietiae insignis is evergreen broad-leaved arbor in the Magnoliaceae Manglietia, tree-like grace, and the four seasons are evergreen, and its leaf is dark green, delicate, keratin, single leaf alternate, be oval shape ellipse, Long Circle or the lanceolar that falls, tree-like grace in great numbers, spend greatly and beautiful, the gorgeous fragrance of pattern, be desirable garden ornamental tree species.Trunk is perfectly straight, wooden good, and bark and branch skin also have good medical value, are again one of rare and endangered tree species that economic worth is very high.Fructus Manglietiae insignis is more original kind, is listed in the famous and precious rare or endangered species of national second class protection.
The natural reproduction year Age of Fructus Manglietiae insignis generally needs 5 years generation seeds of just yielding positive results, due to the impact that is subjected to the factors such as heredity, physiology and environment, naturally solid generation seed seldom, after maturation, attract birds peck at very few.Have now report to take branch as propagating materials carries out cuttage and seedling culture to Fructus Manglietiae insignis, when clip is transplanted a cutting, generally all will retain 1-2 axillalry bud, the branch needed is many, simultaneously, all influential to the tree-like of elite stand and growth when the clip branch.Therefore, usually be subject to the restriction of propagating materials and affect amount reproduction, protection and the exploitation of people to the Fructus Manglietiae insignis plant.
Summary of the invention
The purpose of this invention is to provide a kind of Fructus Manglietiae insignis leaf and insert the method grow seedlings, change that to take branch be the defect that propagating materials is large to the Fructus Manglietiae insignis damage, breeding amount is low, to solve the deficiencies in the prior art.
A kind of Fructus Manglietiae insignis leaf is inserted the method for growing seedlings, the sunny day of mid or late August to mid-September, select the Fructus Manglietiae insignis plant of robust growth without damage by disease and insect, clip is given birth to the lignification branch then, semi-lignified on branch or lignified blade, from related phloem on branch, blade is taken off in the lump, with the petiole end with phloem, in taking root liquid, dip in soon 10-15 second, with little bamboo chip, in base, press 10*10 centimetre distance and make a call to a dark 2-3 centimetre sealing, the blade that will dip in again taking root liquid inserts in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, blade for cuttage can not have any scar.After having inserted, water and take little shed and cover film, and by the surrounding of the film reality of obturaging, before blade is taken root, in little shed, air humidity remains on 80-90%, illumination will be taken sunshade net by force, insert and sprayed again one time taking root liquid in latter 15 days, in conjunction with the bactericidal liquid of spray in every 5-7 days of watering, 0.05-0.1% urea of spray in every 10-15 days and 0.01-0.05% potassium dihydrogen phosphate aqueous solution, left and right petiole base phloem started to break up callus in 20 days, Calli Differentiation went out the former base of root and grew young root in 25-30 days, started in 40-50 days to put out new shoots from petiole and axil punishmentization, through 60-70 days, cultivate and just can carry out transplant planting again.
Described medium is perlite, the peat composed of rotten mosses, mixes and is mixed with medium in the 1:1 ratio.Before inserting 7-10 days with 0.5% potassium permanganate solution to the medium sterilization that carries out disinfection, cover plastic film, after 5-7 days, open film standby.
Described taking root liquid formula is gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, 0.15:0.2:0.005:0.5:0.1:0.1 by ratio of weight and the number of copies, and all the other compositions are sterile water, working concentration is 800-1000 ㎎/L.
Medium material of the present invention, the more careless loose permeability of perlite particle is good, and water suction and the moisture capacity of the peat composed of rotten mosses are relatively good, and the cuttage medium is made in both mixing, not only ventilative but also water conservation, the respiration of favourable cuttage material, thereby hestening rooting.
Potassium permanganate solution carries out sterilizing to seedling bed earth, the multiple diseases of control flowers and trees, and, to the person poultry safety, flowers and trees are difficult for producing poisoning, and noresidue is pollution-free, simultaneously, potassium permanganate also contains potassium, manganese two plant development institute indispensable elements.
Gibberellin: have and promote cell division, elongation, blade to enlarge, stem extends, collateral generation, and breaking dormancy, stratification, the physiological function such as delay senility.
6-benzyl aminopurine: belong to Cytokinin Substances, absorb through root, stem, blade, enter in body, promote cell division, the differentiation of undifferentiated tissue, make cell enlargement, promote seed sprouting, the induced dormancy blastogenesis is long, promote stem, leaf to give birth to elongation and give birth to, the growth of root, break apical dominance, promote the lateral bud growth, suppress chlorophyll and decompose, suppress blade aging, protects green with delay blade flavescence ageing.
Ascorbic acid: participate in redox in electron transport system in plant corpus, promote plant metabolism, aldehydes matter Wheat Protein to the cuttage material production, the aldehydes matter oozed out in the time of suppressing blade cuts causes the autointoxication brown stain, leaf growth is paused, lose differentiation capability.
Glycine: be the constituent of protein, organic nitrogen source can be provided, the differentiation of the growth of cuttage blade and indefinite bud, adventive root is played a driving role.
Glucose: being the main source of life cell institute energy requirement, when being oxidized in vivo carbonic acid gas and water, supplying with heat, is indispensable material in vital movement, can participate in metabolic processes directly, the formation of favourable promotion adventive root.
Fulvic acid: can be absorbed by plant leaf blade, improve respiration, increase chlorophyll content, promote photosynthesis.Reduce the Stoma of Leaves opening degree, reduce the plant leaf blade transpiration, reduce moisture loss, promote plant establishment and Nutrient Absorption.
Due to the photosynthesis of cuttage blade self, can synthesize the material that is conducive to take root, the blade cuttage root-taking is had to facilitation.The blade cuttage and seedling culture is relatively saved the cuttage material, to the utilization of cuttage material be branch cutting 3-5 doubly.Implement the present invention, growth and tree-like impact on former trees while drawing materials are less, simple to operate, and cost is low, easily popularizes, and is convenient to Fructus Manglietiae insignis is carried out to amount reproduction.
Embodiment
Below in conjunction with example, the present invention is described further:
Embodiment 1
A kind of Fructus Manglietiae insignis leaf is inserted the method for growing seedlings: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.Before inserting 7-10 days with after 0.5% potassium permanganate solution processing medium, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, 0.15:0.2:0.005:0.5:0.1:0.1 by ratio of weight and the number of copies, all the other compositions are sterile water, concentration is 1000 ㎎/L.
3, blade is chosen and cuttage: mid-August sunny day, select the Fructus Manglietiae insignis plant of robust growth without damage by disease and insect, clip is given birth to the lignification branch then, semi-lignified on branch or lignified blade, from related phloem on branch, blade is taken off in the lump, with the petiole end with phloem, in taking root liquid, dipped in soon 15 seconds, with little bamboo chip, by 10*10 centimetre distance, making a call to a degree of depth in base is 2-3 centimetre sealing, the blade that will dip in again taking root liquid inserts in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, for the blade of cuttage, any scar can not be arranged.Often having inserted behind one case seedbed waters takes little shed and covers film, and by the surrounding of the film reality of obturaging.
4, after cuttage, manage: before blade was taken root, air humidity remained on 80-90% in little shed, and with the clear water blade of spraying, medium is protected special moisture state, will take sunshade net when illumination is strong in case of necessity.Insert and sprayed again taking root liquid, hestening rooting and a germination in latter 15 days.In conjunction with the bactericidal liquid of spray in every 5-7 days of watering, 0.05-0.1% urea of spray in every 10-15 days and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Left and right petiole base phloem started to break up callus in 20 days, and Calli Differentiation went out the former base of root and grew young root in 25-30 days, and 40-50 days start to put out new shoots from petiole and axil punishmentization, then cultivated and just can carry out transplant planting through 60-70 days.
Embodiment 2
A kind of Fructus Manglietiae insignis leaf is inserted the method for growing seedlings: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.Before inserting 7-10 days with after 0.5% potassium permanganate solution processing medium, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, 0.15:0.2:0.005:0.5:0.1:0.1 by ratio of weight and the number of copies, all the other compositions are sterile water, concentration is 900 ㎎/L.
3, blade is chosen and cuttage: late August sunny day, select the Fructus Manglietiae insignis plant of robust growth without damage by disease and insect, clip is given birth to the lignification branch then, semi-lignified on branch or lignified blade, from related phloem on branch, blade is taken off in the lump, with the petiole end with phloem, in taking root liquid, dipped in soon 12 seconds, with little bamboo chip, in base, press 10*10 centimetre distance and make a call to a sealing, the blade that will dip in again taking root liquid inserts in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, for the blade of cuttage, any scar can not be arranged.Often having inserted behind one case seedbed waters takes little shed and covers film, and by the surrounding of the film reality of obturaging.
4, after cuttage, manage: before blade was taken root, air humidity remained on 80-90% in little shed, and when humidity reached, with the clear water blade of spraying, medium was not protected special moisture state, will take sunshade net when illumination is strong.Insert and sprayed again taking root liquid, hestening rooting and a germination in latter 15 days.In conjunction with the bactericidal liquid of spray in every 5-7 days of watering, 0.05-0.1% urea of spray in every 10-15 days and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Left and right petiole base phloem started to break up callus in 25 days, and the left and right Calli Differentiation went out the former base of root and grew young root in 30 days, and 40-50 days start to put out new shoots from petiole and axil punishmentization, then cultivated and just can carry out transplant planting through 60-70 days.
Embodiment 3
A kind of Fructus Manglietiae insignis leaf is inserted the method for growing seedlings: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.Before inserting 7-10 days with after 0.5% potassium permanganate solution processing medium, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, 0.15:0.2:0.005:0.5:0.1:0.1 by ratio of weight and the number of copies, all the other compositions are sterile water, concentration is 1000 ㎎/L.
3, blade is chosen and cuttage: early September sunny day, select the Fructus Manglietiae insignis plant of robust growth without damage by disease and insect, clip is given birth to the lignification branch then, semi-lignified on branch or lignified blade, from related phloem on branch, blade is taken off in the lump, with the petiole end with phloem, in taking root liquid, dipped in soon 10 seconds, with little bamboo chip, in base, press 10*10 centimetre distance and make a call to a sealing, the blade that will dip in again taking root liquid inserts in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, for the blade of cuttage, any scar can not be arranged.Often having inserted behind one case seedbed waters takes little shed and covers film, and by the surrounding of the film reality of obturaging.
4, after cuttage, manage: before blade was taken root, air humidity remained on 80-90% in little shed, and when humidity reached, with the clear water blade of spraying, medium was not protected special moisture state, will take sunshade net when illumination is strong.Insert and sprayed again taking root liquid, hestening rooting and a germination in latter 15 days.In conjunction with the bactericidal liquid of spray in every 5-7 days of watering, 0.05-0.1% urea of spray in every 10-15 days and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Left and right petiole base phloem started to break up callus in 25 days, and Calli Differentiation went out the former base of root and grew young root in 30-35 days, and 40-50 days start to put out new shoots from petiole and axil punishmentization, then cultivated and just can carry out transplant planting through 60-70 days.
Embodiment 4
A kind of Fructus Manglietiae insignis leaf is inserted the method for growing seedlings: comprise the following steps:
1, medium preparation: be hybridly prepared into medium in 1:1 ratio perlite, grass.Before inserting 7-10 days with after 0.5% potassium permanganate solution processing medium, cover plastic film, after 5-7 days, open film.
2, taking root liquid preparation: gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, 0.15:0.2:0.005:0.5:0.1:0.1 by ratio of weight and the number of copies, all the other compositions are sterile water, concentration is 800 ㎎/L.
3, blade is chosen and cuttage: mid-September sunny day, select the Fructus Manglietiae insignis plant of robust growth without damage by disease and insect, clip is given birth to the lignification branch then, semi-lignified on branch or lignified blade, from related phloem on branch, blade is taken off in the lump, with the petiole end with phloem, in taking root liquid, dipped in soon 15 seconds, with little bamboo chip, in base, press 10*10 centimetre distance and make a call to a sealing, the blade that will dip in again taking root liquid inserts in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, for the blade of cuttage, any scar can not be arranged.Often having inserted behind one case seedbed waters takes little shed and covers film, and by the surrounding of the film reality of obturaging.
4, after cuttage, manage: before blade was taken root, air humidity remained on 80-90% in little shed, and when humidity reached, with the clear water blade of spraying, medium was not protected special moisture state, will take sunshade net when illumination is strong.Insert and sprayed again taking root liquid, hestening rooting and a germination in latter 15 days.In conjunction with the bactericidal liquid of spray in every 5-7 days of watering, 0.05-0.1% urea of spray in every 10-15 days and 0.01-0.05% potassium dihydrogen phosphate aqueous solution.Left and right petiole base phloem started to break up callus in 30-35 days, and Calli Differentiation went out the former base of root and grew young root in 40-45 days, and 40-50 days start to put out new shoots from petiole and axil punishmentization, then cultivated and just can carry out transplant planting through 60-70 days.
Practice result shows: the taking root liquid of several concentration all can make to produce Calli Differentiation after the blade cuttage, and average rooting rate reaches 73.6%, is up to 86%.The concentration of taking root is best with the effect of 15 seconds with mid-August, processing time with 1000 ㎎/L, cutting time.
Blade cuttage statistical effect table
Claims (3)
1. a Fructus Manglietiae insignis leaf is inserted the method for growing seedlings, it is characterized in that: the sunny day of mid or late August to mid-September, select the Fructus Manglietiae insignis plant of robust growth without damage by disease and insect, clip is given birth to the lignification branch then, semi-lignified on branch or lignified blade, from related phloem on branch, blade is taken off in the lump, with the petiole end with phloem, in taking root liquid, dip in soon 10-15 second, with little bamboo chip, in base, press 10*10 centimetre distance and make a call to a dark 2-3 centimetre sealing, the blade that will dip in again taking root liquid inserts in medium, insertion depth is together with petiole 2-3 centimetre, compress medium, blade for cuttage can not have any scar.After having inserted, water and take little shed and cover film, and by the surrounding of the film reality of obturaging, before blade is taken root, in little shed, air humidity remains on 80-90%, illumination will be taken sunshade net by force, insert and sprayed again one time taking root liquid in latter 15 days, in conjunction with the bactericidal liquid of spray in every 5-7 days of watering, 0.05-0.1% urea of spray in every 10-15 days and 0.01-0.05% potassium dihydrogen phosphate aqueous solution, left and right petiole base phloem started to break up callus in 20 days, Calli Differentiation went out the former base of root and grew young root in 25-30 days, started in 40-50 days to put out new shoots from petiole and axil punishmentization, through 60-70 days, cultivate and just can carry out transplant planting again.
2. a kind of Fructus Manglietiae insignis leaf as claimed in claim 1 is inserted the method for growing seedlings, and it is characterized in that: medium is perlite, the peat composed of rotten mosses, mixes and is mixed with medium in the 1:1 ratio.Before inserting 7-10 days with 0.5% potassium permanganate solution to medium disinfection medium after, cover plastic film, after 5-7 days, open film.
3. a kind of Fructus Manglietiae insignis leaf as claimed in claim 1 or 2 is inserted the method for growing seedlings, it is characterized in that: the taking root liquid formula is gibberellin, 6-benzyl aminopurine, ascorbic acid, glycine, glucose, fulvic acid, 0.15:0.2:0.005:0.5:0.1:0.1 by ratio of weight and the number of copies, working concentration is 800-1000 ㎎/L, and all the other compositions are sterile water.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104813939A (en) * | 2015-02-05 | 2015-08-05 | 南京农业大学 | Method for constructing lotus regeneration system |
| CN105409780A (en) * | 2015-12-30 | 2016-03-23 | 云南飞熊农业科技有限公司 | Plant tissue culture medium without disinfection and preparation method therefor |
| CN105432280A (en) * | 2015-11-26 | 2016-03-30 | 全椒县桃花湾果蔬种植专业合作社 | Cultivation method for potting glechoma hedracea |
| CN104641921B (en) * | 2015-03-20 | 2016-08-17 | 日照恒久丰农业科技有限公司 | The blade cuttage breeding method of somewhat-white magnolia |
| CN106034591A (en) * | 2016-05-26 | 2016-10-26 | 宜宾云辰乔木园林有限责任公司 | Method for prompting blossom of manglietia insignis |
| CN106795510A (en) * | 2014-07-11 | 2017-05-31 | 莫迪卡戈公司 | The albumen improved in plant is produced |
| CN112544444A (en) * | 2020-12-03 | 2021-03-26 | 中国科学院昆明植物研究所 | Tissue culture medium for manglietia insignis, method for culturing embryonic callus of manglietia insignis and method for rapidly propagating manglietia insignis |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106795510A (en) * | 2014-07-11 | 2017-05-31 | 莫迪卡戈公司 | The albumen improved in plant is produced |
| CN104813939A (en) * | 2015-02-05 | 2015-08-05 | 南京农业大学 | Method for constructing lotus regeneration system |
| CN104641921B (en) * | 2015-03-20 | 2016-08-17 | 日照恒久丰农业科技有限公司 | The blade cuttage breeding method of somewhat-white magnolia |
| CN105432280A (en) * | 2015-11-26 | 2016-03-30 | 全椒县桃花湾果蔬种植专业合作社 | Cultivation method for potting glechoma hedracea |
| CN105409780A (en) * | 2015-12-30 | 2016-03-23 | 云南飞熊农业科技有限公司 | Plant tissue culture medium without disinfection and preparation method therefor |
| CN105409780B (en) * | 2015-12-30 | 2019-03-29 | 玉溪市飞熊农业开发有限公司 | A kind of plant tissue culture culture medium and preparation method thereof without sterilizing |
| CN106034591A (en) * | 2016-05-26 | 2016-10-26 | 宜宾云辰乔木园林有限责任公司 | Method for prompting blossom of manglietia insignis |
| CN106034591B (en) * | 2016-05-26 | 2019-04-05 | 宜宾云辰乔木园林有限责任公司 | A method of Fructus Manglietiae insignis is bloomed ahead of time |
| CN112544444A (en) * | 2020-12-03 | 2021-03-26 | 中国科学院昆明植物研究所 | Tissue culture medium for manglietia insignis, method for culturing embryonic callus of manglietia insignis and method for rapidly propagating manglietia insignis |
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Address after: Yibin City, Sichuan province 644000 Qinghua Street No. 8 Building 1 Cuiping District 2 floor No. 1-4 Patentee after: Sichuan Yunchen Garden Technology Co., Ltd Address before: 644000, Yibin, Sichuan Province on the South Bank of the west side Tiancheng Yuan District 16 1F-1 Patentee before: Yibin Yunchen Arbor Garden Co., Ltd. |