CN104160878B - A kind of method of doing the color leaf Chinese ilex of stock grafting with Dabie Mountain Chinese ilex - Google Patents
A kind of method of doing the color leaf Chinese ilex of stock grafting with Dabie Mountain Chinese ilex Download PDFInfo
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- CN104160878B CN104160878B CN201310277677.5A CN201310277677A CN104160878B CN 104160878 B CN104160878 B CN 104160878B CN 201310277677 A CN201310277677 A CN 201310277677A CN 104160878 B CN104160878 B CN 104160878B
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Abstract
The invention provides a kind of method of doing the color leaf Chinese ilex of stock grafting with Dabie Mountain Chinese ilex; Select Dabie Mountain Chinese ilex to do stock, do scion with color leaf Chinese ilex new varieties, by the method for cut-grafting, by Dabie Mountain Chinese ilex stock and the scion of color leaf Chinese ilex new varieties, the two forms layer alignment, makes it grafting survival; The present invention has strengthened the adaptability of color leaf Chinese ilex new varieties, and stock is easy to get, and cost is low, has improved breeding coefficient, has expanded output, makes the large-scale production of color leaf Chinese ilex new varieties become possibility.
Description
Technical field
The invention belongs to xylophyta seedling reproduction technique field, be specifically related to one and make the color leaf of stock grafting with Dabie Mountain Chinese ilexThe method of Chinese ilex.
Background technology
Color leaf Chinese ilex (Ilexsp.) belongs to Aquifoliaceae (Aquifoliaceae) Ilex (Ilex) plant. It is a class leaf lookThe evergreen dungarunga of beautiful colour or shrub, plant height 2-6 rice, blade edge colour band Phnom Penh, Yin Bian or Jin Xin etc. are glossy, avetteTo ovum shape ellipse, long 2-4 centimetre, wide is 2-3 centimetre, spends green white, fruit ruby look spherical, 8 millimeters, footpath, florescence 4-6Month, the fruit phase 10-12 month. Like warmly, moistening, the environment that light is good, requires fertile soil, and draining is good, organic to be rich inThe sand loam of matter is advisable, and the abundant watering, fertilizing of season of growing, in order to its well-grown. This type of seeds tree crown spheroidal, Ye SeqingEmerald green band color, haw is innumerable, is good four seasons ornamental tree species, can isolated planting, group plants or sheet is planted and done landscape tree, shade tree, suitableYing Xingguang, contamination resistance is stronger, also can be used for street corner, highway Lutao, factories and miness district etc. and locates greening.
Dabie Mountain Chinese ilex (IlexdabieshanensisKYao.etM.P.Deng.) is that the Da Bie Mountain areas such as China Anhui originate inEvergreen Broad-leaved Tree Species. Evergreen dungarunga, height is met rice, and 30 centimetres of the diameters of a cross-section of a tree trunk 1.3 meters above the ground are distributed in the regional height above sea level 1000 such as Anhui and JiangxiIn valley floor, low gully, hills or hillside woods that rice is following; For shade tolerance seeds, but need certain illumination to the strong length of time. Deep-rootedness, anti-Wind. The 4-6 month blooms, and fruit is ripe in November. The large dignity of Dabie Mountain holly height, grandness, tree crown is neat, with luxuriant foliage and spreading branches in leafy profusion, the four seasonsEvergreen, in the afforestation of South China Urban, can be used as shade tree, shade tree, landscape tree, also can do in vast HillsFor desirable reproducting tree species.
Current color leaf Chinese ilex seedling quantity is considerably less at home, and it is due to its breediness, and therefore, seeding and seedling raising germination percentage is very low,And germinating time is long, 2-3 and could germinate even for more time, so seed seedling-raising is very difficult; And breediness is notCan keep stable. Breed difficulty and not only the nursery stock development of color leaf Chinese ilex is formed to certain restriction, and the drawback causing is, businessProduct cultivation period is elongated, has increased seedling cost; The best period that postpones commercial seedling listing, affects economic benefit.
Propagation by grafiting is in current industry, to solve one of main path of nursery stock production. Existing engrafting method, conventionally by the color leaf winterBlue or green new varieties scion is after parent is taken off, and grafting is on the stocks such as Chinese holly, and these stocks are subject to the harm such as scale insect, and pipe is supportedCost is relatively high. Suit measures to local conditions, cost-saving, large area industrialization makes the color leaf Chinese ilex of stock grafting new product with Dabie Mountain Chinese ilexThe method of planting yet there are no report.
The feature of Dabie Mountain Chinese ilex is strong adaptability, and growth is fast, resistance, and disease and pest is few, and optimum is made other new excellent Chinese ilex productKind graft stock, can either promote fast scion grafting survival, growing after can promoting again grafting to survive. Utilize largeOther mountain Chinese ilex makes the color leaf Chinese ilex of stock grafting new varieties, can obtain fast and effectively a large amount of color leaf Chinese ilex new varieties seedlings, solvesThe problem of color leaf Chinese ilex new varieties shortage of resources; By to stock, scion and survive the reasonable selection of rear management method, can makeObtain color leaf Chinese ilex new varieties graft survival rate and reach more than 95%, survive the rear seedling specification (more than plant height 60cm) that reaches fast,Can efficiently obtain color leaf Chinese ilex new varieties high quality seedling, greatly meet the demand in market.
Summary of the invention
The object of the invention is to overcome the deficiency of prior art and a kind of simple, convenient, injured light, grafting place of stock is providedEasily healing, survival rate is high, efficiency is high, and at the easily Chinese ilex kind engrafting method of row of practicality.
Applicant is engaged in the work of growing seedlings of color leaf Chinese ilex for many years, for meeting the demand of production aspect, and our side to conventional graftingMethod improves, and engrafting method is innovated, the object that shorten growing-seedling period to reach, increases the quantity of growing seedlings, thisBright object can reach by following measure: a kind of to do the grafting of the color leaf Chinese ilex of stock grafting new varieties with Dabie Mountain Chinese ilex numerousCulturing method, is characterized in that rootstock seedling to cut off apart from the above branches and leaves part in 5～8 centimetres, ground, removes dividing below clipBranch, selects smooth smooth position, downward from clip, with cutting point by long cortex rip cutting one 1～2 centimetre of dark the perpendicular of xylem that reachesMouthful, otch upper end cortex, with cutting point strip off gently to both sides, then will be retained to 3.5-4 centimetre under scion bud, on bud, retain 8～10 millimeters, in the side of bud or the back side be whittled into the peltate inclined-plane, garden of long 2～2.5 centimetres, make it not injure xylem and exposeBast, the xylem by the inclined-plane of scion towards stock, slowly inserts the subcutaneous of stock, makes the top, inclined-plane of scion show money or valuables one carries unintentionally 2～3 millimeters, by interface tightly winding wrapping from bottom to top, beat button tighten in the bud upper part of scion with farm non-drop film.
Particularly, the present invention is that the technical scheme that its technical problem of solution is taked is:
A propagation by grafiting method of making the color leaf Chinese ilex of stock grafting new varieties with Dabie Mountain Chinese ilex, is characterized in that, comprises followingStep:
A) stock processing; B) scion processing; C) grafting time; D) engrafting method; E) managing after grafting.
It is characterized in that:
Steps A) in stock processing procedure be: in ground, garden, select robust growth, without the raw Dabie Mountain winter of the 2-3 of disease and pestThe grafting that blue or green leading thread rugosity is 0.3-1cm, as graft stock, cuts off rootstock seedling apart from the above branches and leaves in 5～8 centimetres, groundPart, removes the branch below clip, selects smooth smooth position, downward from clip, with cutting point by cortex rip cutting one long 1～2 centimetres dark reaches perpendicular mouthful of xylem, by otch upper end cortex with cutting the sharp strip off gently to both sides;
Step B) in scion processing, choose the current-year branch of color leaf Chinese ilex new varieties, the truncation of scion upper cut, lower cutCut sth. askew; Length is 3-5em, and plant growth regulator soaks, and described plant growth regulator is: 5 parts of indolebutyric acids, diethyl aminoethyl hexanoate(DA-6) 3 parts, 2 parts of gibberellin, KT-301 part, VB11 part, with the dilution ratio preparation plant of 150mg: 1LGrowth mediator agent, then soaks branch bottom 15min with described plant growth mediator agent+1%DMSO,
Step C) in the grafting time that adopts be: in general, be suitable in comparatively in good time 2 months of temperature, the last ten-days period;
Step D) in the engrafting method that adopts be: use the method for scion grafting, preferably cut-grafting is comparatively applicable;
Step e) in adopt managing after grafting method be: attention moisturizing after grafting completes, prevents mechanical damage; Often note observing,Maintenance, 40d after grafting, uses nutrient solution to spray plant blade face, according to grafting growth characteristics, respectively in peak period, speedRaw phase, growth later stage are carried out foliage top dressing. The 1st minor peaks phase, (April, nursery stock started growth period) was every 5d foliage-spray0.1%-0.3% calcium superphosphate and potassium dihydrogen phosphate, promote seedling root to grow and stem lignifying. The 2nd fast-growingPhase (the 7-8 month), every 5-7d foliage-spray 0.1%-0.2% urea liquid, promote seedling growth. The seedling growth later stage,Every 5-7d foliage-spray 0.3% potassium dihydrogen phosphate or 0.2%-0.3% potassium sulfate solution, promote nursery stock lignifying, strengthen moreWinter cold tolerance.
The beneficial effect of the relative prior art of the present invention is:
1: compared with the prior art the present invention has following good effect: due to when the grafting with prune the gently back of the body on inclined-plane of knifeThe Lao Pi of face both sides, makes it to expose bast, but does not injure xylem, and engrafting method is effectively discharged and hindered liquid, promotes the contact of anvil fringeFace forms callus as early as possible, ensures the viability of scion, and the positive director's gesture of selected stock vigorous period, moisture and nutrition recombination,Endogenous Hormone Contents in Vitro is high, and stock and scion affinity good, therefore, greatly promoted graft survival rate. By the inclined-plane court of scionTo the xylem of stock, slowly insert the subcutaneous of stock, with regard to being in contact with one another, the bast of stock and scion compares prior art like thisSimple wooden contact is easily healed, and survival rate is high, and can grafting in three seasons of spring and summer autumn: this slotting skin grafting is also than existing skillArt is simple, convenient, efficiency is high.
2: existing engrafting method, conventionally by the scion of color leaf Chinese ilex new varieties after parent is taken off, grafting is at anvils such as Chinese hollysOn wood, the affinity of these stocks and color leaf Chinese ilex is not strong, a little less than producing the ability of callus between stock and scion, stock with connectThe conduit of fringe, screen casing can not UNICOMs, and new conducting tissue is difficult to form, and the substances transport such as moisture and nutrition are not in place, and are subject to be situated betweenThe harm such as shell worm, pipe is formed originally relatively high, and present inventor finds, for the grafting of color leaf Chinese ilex new varieties, uses largeOther mountain Chinese ilex makes stock, and affiliation and affinity the best have greatly shortened and cultivated the foster time of color leaf Chinese ilex new product seedling pipe and becomeThis;
Respectively taking Chinese holly and Dabie Mountain Chinese ilex as stock, the color leaf Chinese ilex of Na in grafting drag-line, all experiments were is that same grafting work is transferredConnect, scion and engrafting method all with, investigate different stocks to the color leaf Chinese ilex of Na in grafting drag-line survival rate, the new slightly impact of lengthAs follows:
| Stock kind | Quantity of the scion | Survival rate | On average new length slightly |
| Chinese holly | 103 | 81％ | 9.15±2.67 |
| Dabie Mountain Chinese ilex | 107 | 96％ | 13.23±2.25 |
The different stock of visible employing to the grafting growth of the color leaf Chinese ilex of Na in drag-line significantly, this with drag-line in the color leaf Chinese ilex of Na andThe affiliation distance of Chinese holly, Dabie Mountain Chinese ilex and the difference of affinity are relevant;
The feature of Dabie Mountain Chinese ilex is strong adaptability, and growth is fast, resistance, and disease and pest is few, and optimum is made other new excellent Chinese ilex productKind graft stock parent, can either promote fast scion grafting survival, growing after can promoting again grafting to survive. ProfitMake the color leaf Chinese ilex of stock grafting new varieties in order to Dabie Mountain Chinese ilex, can obtain fast and effectively a large amount of color leaf Chinese ilex new varieties seedlings,Solve the problem of color leaf Chinese ilex new varieties shortage of resources; By to stock, scion and survive the reasonable selection of rear management method, energyEnough make color leaf Chinese ilex new varieties graft survival rate reach more than 95%, survive rear reach fast seedling specification (plant height 60cm withOn), can obtain efficiently color leaf Chinese ilex new varieties high quality seedling, greatly meet the demand in market.
3 plant growth regulator are a kind of ectogenic non-nutritive chemical substances, and it can conduct to effect conventionally in plantPosition, just can promote or suppress some link in plant life process with lower concentration, makes it to the meaning that meets human needsBe willing to development; Present inventor show that by lot of experiments effect is remarkable, described plant through plant growth regulator immersionGrowth regulator is: 5 parts of indolebutyric acids, 3 parts of diethyl aminoethyl hexanoates (DA-6), 2 parts of gibberellin, KT-301 part, VB11Part, with the dilution ratio preparation plant growth mediator agent of 150mg: 1L, and DMSO is cytoactive component, adds 1%DMSOAfter, soak branch bottom 15min, after this processing, the time of sprouting to buddering from grafting shortens greatly, and single-factor is tested and is drawn,Than the time decreased 39.5% without any processing, plant growth mediator agent formula of the present invention, it is easy to use, canImprove callus activity, promote callus growth, promoting budders sprouts, and improves the chlorophyll content of scion, strengthens photosyntheticEffect, and the auxin rational proportion containing, Coordinated Interaction, has the functions such as the root hair cell of promotion division, effectively carriesGrowing of coca hair, obviously shortens seedling-slowing stage, tachyauxesis.
4 utilize high-voltage electrostatic field to process scion, and the motion that it has affected permeability and other polar molecule of film, has improved cellAbsorption and transhipment physical capacity, thereby can promote the growth of tissue. It not only can strengthen improving activity of root system, increases the life of rootThing amount, improves cuttage survival rate, and promote growth of seedling, but also can strengthen premunition, but, utilize high-voltage electrostatic field placeReason cuttings, different kind numerical value differ larger, and different intensity and ageing can produce different effects, if process electricityField intensity is excessive, or the processing time is long, can make plant cell come to harm even dead, and electric-field intensity is too small or the timeToo short, can cause again treatment effect not obvious, present inventor draws for color leaf Chinese ilex and adopts intensity through creative workFor 50KV/cm, the treatment effect of 0.5 hour time is best, root growth rate, scion survival rate, and vitality index numerical value maximum,Conductivity values is minimum, and every physical signs is extreme value.
When 5 grafting, adopted farm non-drop film to twine to tie up fixing in grafting part, this film has good elasticity, has avoidedWhen stock carries out girth growth, strangle trace, can reach and exempt from solution and tie up object; And film has powerful binding force to top scion, waitingFringe grows into after branch, and the effect that film plays the newborn branch of protection in the time blowing twines the film of tying up and do not need solution to tie up, the film stayingCan, by fixing tight to scion and stock, play protection effect, effectively reduce the amount of labour and production cost in grafting production;
Detailed description of the invention
Embodiment mono-
Rootstock seedling is wiped out apart from the above part in 8 centimetres, ground, is removed the branch below clip, select smooth smooth position,Downward from clip, with cutting point by long cortex rip cutting one 2 centimetres of dark skilful mouthfuls of reaching xylem, otch upper end cortex is sharp to two with cuttingLimit is strip off gently, then will under scion bud, retain 4 centimetres, retains 1 centimetre on bud, is whittled into long 25 at side or the back side of budThe buckler-shaped inclined-plane of millimeter, then with prune the gently Lao Pi of both sides, the back side on inclined-plane of knife, makes it not injure xylem and exposes toughSkin zone, the xylem by the inclined-plane of scion towards stock, slowly inserts the subcutaneous of stock, makes the top, inclined-plane of scion show money or valuables one carries unintentionally 3 in the leastRice, with without dripping film band, interface being tightly wound around to wrapping from bottom to top, beats button tighten in the bud upper part of scion,
1, choosing of stock: choosing 60 strain leading thread rugosity in ground, garden is 0.3-1cm, robust growth, raw without the 2-3 of disease and pestDabie Mountain Chinese ilex (IlexdabieshanensisKYao.etM.P.Deng.) grafting is as graft stock;
2, scion processing: on February 23rd, 2012, choose 60 stalwartnesses, give birth to the color leaf Chinese ilex of Na in drag-line without the 2-3 of disease and pest(Ilexxaltaclerensis ' Lawsoniana ') seedling current-year branch is as scion. The truncation of scion upper cut, incisionMouth is cut sth. askew, otch is level and smooth, crack-free; Length is 2-3cm; Scion lower cut place should be internode side with the adventitious root primordia of hidingBranch base portion, adventitious bud base portion or petiole base; Scion retains 1 pair of blade, and cuts off a half vane, and has complete full sideBud. After scion is ready to, first process (HVEF) with high-voltage electrostatic field, intensity is 50KV/cm, and the time is 30min;Then first soak and soak respectively with DMSO with plant growth regulator, described plant growth regulator is: 5 parts of indolebutyric acids,3 parts of diethyl aminoethyl hexanoates (DA-6), 2 parts of gibberellin, KT-301 part, VB11 part, joins with the dilution ratio of 150mg: 1LPlant growth regulator processed, then soaks respectively branch bottom 15min with described plant growth regulator, 1%DMSO;
3, engrafting method: use cut-grafting method. Scion surface temperature control is being 20 DEG C, relative humidity 85%.
4,20d after grafting, scion base portion starts the callus of growing.
5, fertilizing management: 40d after grafting, uses nutrient solution to spray plant blade face, according to grafting growth characteristics, respectively at peakValue phase, fast-growing phase, growth later stage are carried out foliage top dressing. The 1st minor peaks phase, (April, nursery stock started growth period) was every 5d blade faceSpray 0.1%-0.3% calcium superphosphate and potassium dihydrogen phosphate, promote seedling root to grow and stem lignifying. The 2nd timeThe fast-growing phase (the 7-8 month), every 5-7d foliage-spray 0.1%-0.2% urea liquid, promote seedling growth. After seedling growthPhase, every 5-7d foliage-spray 0.3% potassium dihydrogen phosphate or 0.2%-0.3% potassium sulfate solution, promotes nursery stock lignifying, increasesThe cold tolerance of surviving the winter by force.
6,22d after grafting, grafting is extracted the tender tip out, 35d after grafting, tender tip exhibition leaf is complete, and plant height on average reaches 13.75cm,Branch amount on average reaches 4.
Embodiment bis-
Rootstock seedling is wiped out apart from the above part in 5 centimetres, ground, is removed the branch below clip, select smooth smooth position,Downward from clip, with cutting point by long cortex rip cutting one 1 centimetre of dark perpendicular mouthful of reaching xylem, otch upper end cortex is sharp to two with cuttingLimit is strip off gently, then will under scion bud, retain 3.5 centimetres, retains 8 millimeters on bud, is whittled into long 2 at side or the back side of budCentimetre buckler-shaped inclined-plane, then with prune the gently Lao Pi of both sides, the back side on inclined-plane of knife, make it not injure xylem and expose toughSkin zone, the xylem by the inclined-plane of scion towards stock, slowly inserts the subcutaneous of stock, makes the top, inclined-plane of scion show money or valuables one carries unintentionally 2 in the leastRice, with without dripping film, interface being tightly wound around to wrapping from bottom to top.
1, choosing of stock: choosing 60 strain leading thread rugosity in ground, garden is 0.3-1cm, robust growth, raw large without the 2-3 of disease and pestOther mountain Chinese ilex (IlexdabieshanensisKYao.etM.P.Deng.) grafting is as graft stock
2, scion processing: on February 27th, 2012, choose 60 stalwartnesses, give birth to golden-rimmed serrate holly leaf (Ilex without the 2-3 of disease and pestAquifoliumL. ' GoldenVonTol ') seedling current-year branch is as scion. The truncation of scion upper cut, lower cut are obliqueCut, otch is level and smooth, crack-free; Length is 2-3cm; Scion lower cut place should be internode side shoot base with the adventitious root primordia of hidingPortion, adventitious bud base portion or petiole base; Scion retains 1 pair of blade, and cuts off a half vane, and has complete full lateral bud.After scion is ready to, keep moistening, be positioned over shady and cool moistening place, after scion is ready to:
First process (HVEF) with high-voltage electrostatic field, intensity is 50KV/cm, and the time is 30min;
Then first soak and then soak respectively with DMSO with plant growth regulator, described plant growth regulator is: indolebutyric acid 5Part, 3 parts of diethyl aminoethyl hexanoates (DA-6), 2 parts of gibberellin, KT-301 part, VB11 part, with the thinner ratio of 150mg: 1LExample preparation plant growth regulator, soaks respectively branch bottom 15min with described plant growth regulator, 1%DMSO;
3, engrafting method: use cut-grafting method. Scion surface temperature control is being 22 DEG C, relative humidity 85%.
4,18d after grafting, scion wound portion starts the callus of growing.
5, fertilizing management: 20d after grafting, use nutrient solution to spray plant blade face, nutrient composition is: N180mg/L, P25mg/L,K50mg/L, phosphate fertilizer is with NH4H2PO4Form adds, and potash fertilizer is with KNO3Form adds, and nitrogenous fertilizer is divided by NH4H2PO4And KNO3Form add outside, (NH for insufficient section4)2SO4Supplement. Use sprayer to carry out Foliar Spraying, volume is 2L/m2。Grafting 60d and 75d, each repeat spraying 1 time.
6,20d after grafting, grafting is extracted the tender tip out, 40d after grafting, tender tip exhibition leaf is complete, and plant height on average reaches 15.5cm,Branch amount on average reaches 3-4.
Embodiment tri-:
Rootstock seedling is wiped out apart from the above part in 6.5 centimetres, ground, removed the branch below clip, select smooth smooth portionPosition, downward from clip, with cutting point by long cortex rip cutting one 1.5 centimetres of dark perpendicular mouthfuls of reaching xylem, by otch upper end cortex with cuttingPoint strip off gently to both sides, then will retain 3.7 centimetres under scion bud, retain 9 millimeters on bud, cuts at side or the back side of budThe grow up horse ear shape inclined-plane of 2.2 centimetres, then with prune the gently Lao Pi of both sides, the back side on inclined-plane of knife, make it not injure woodenPortion and expose bast, the xylem by the inclined-plane of scion towards stock, slowly inserts the subcutaneous of stock, makes on the inclined-plane of scionPortion shows money or valuables one carries unintentionally 2.5 millimeters, with without dripping film band, interface being tightly wound around to wrapping from bottom to top, beats button tighten in the bud upper part of scion.
1, choosing of stock: choosing 60 strain leading thread rugosity in ground, garden is 0.3-1cm, robust growth, raw large without the 2-3 of disease and pestOther mountain Chinese ilex (IlexdabieshanensisKYao.etM.P.Deng.) grafting is as graft stock.
2, scion processing: on February 26th, 2012, choose 40 stalwartnesses, without the color leaf Chinese ilex of the 2-3 Sheng Yinbian of disease and pest (IlexAquifoliumL. ' Argentea-marginata ') seedling current-year branch is as scion. The truncation of scion upper cut, lower cutCut sth. askew, otch is level and smooth, crack-free; Length is 2-3cm; Scion lower cut place should be internode side shoot with the adventitious root primordia of hidingBase portion, adventitious bud base portion or petiole base; Scion retains 1 pair of blade, and cuts off a half vane, and has complete full lateral bud.After scion is ready to, keep moistening, be positioned over shady and cool moistening place. After scion is ready to:
First process (HVEF) with high-voltage electrostatic field, intensity is 50KV/cm, and the time is 30min;
Then first soak and then soak respectively with DMSO with plant growth regulator, described plant growth regulator is: indolebutyric acid 5Part, 3 parts of diethyl aminoethyl hexanoates (DA-6), 2 parts of gibberellin, KT-301 part, VB11 part, with the thinner ratio of 150mg: 1LExample preparation plant growth regulator, soaks respectively branch bottom 15min with described plant growth regulator, 1%DMSO;
3, engrafting method: use cut-grafting method. Scion surface temperature control is being 24 DEG C, relative humidity 80%.
4,20d after grafting, scion wound portion starts the callus of growing.
5, fertilizing management: after grafting survives, should execute thin composite fertilizer, nitrogen, phosphorus, potassium three ratio are 1: 1: 1. Children's teaFertilising, adheres to " having many meals but little food at eath ", and each dose is few but often. To the tree that grows into forest, many nitrogen fertilizer applications, nitrogen, phosphorus, potassium ratioExample is 3: 1: 1. Impose quick-acting fertilizer should be before spring bud be sprouted and spring and summer tea shoot pluck and carry out later, slow-release fertilizer (as calcium superphosphate,Bone meal, pond sludge etc.) be suitable at the beginning of the autumn and winter and discharge in conjunction with deep ploughing. To use emphatically farm organic fertilizer material.
6,20d after grafting, grafting is extracted the tender tip out, 38d after grafting, tender tip exhibition leaf is complete, and plant height on average reaches 15.5cm,Branch amount on average reaches 3-4.
Make the color leaf Chinese ilex of stock grafting new varieties method according to the disclosed utilization of the application with Dabie Mountain Chinese ilex, can either promote fastScion healing survives, and can promote again the growth of Grafted Variety seedling after grafting survival. Utilize with Dabie Mountain Chinese ilex and make the color leaf of stock graftingChinese ilex new varieties, can obtain a large amount of color leaf Chinese ilex new varieties seedlings fast and effectively, solve color leaf Chinese ilex new varieties shortage of resourcesProblem; By to stock, scion and survive the reasonable selection of rear management method, can make color leaf Chinese ilex new varieties grafting becomeMotility rate reaches more than 95%, survives the rear seedling specification (more than plant height 40cm) that reaches fast, can obtain efficiently the color leaf winterBlue or green new varieties high quality seedling, has met the demand in market greatly.
Although above the present invention is described in detail with a general description of the specific embodiments, in the present inventionOn basis, can make some modifications or improvements it, this will be apparent to those skilled in the art. Therefore, notDepart from these modifications or improvements on the basis of spirit of the present invention, all belong to the scope of protection of present invention.
Claims (1)
1. a method of doing the color leaf Chinese ilex of stock grafting with Dabie Mountain Chinese ilex, is characterized in that, taking Dabie Mountain Chinese ilex as stock, and color leafChinese ilex is scion, and described color leaf Chinese ilex is the color leaf Chinese ilex of Na (Ilexxaltaclerensis ' Lawsoniana ') in drag-line, comprise withLower step:
A) stock processing; B) scion processing; C) grafting time; D) engrafting method; E) managing after grafting;
Described steps A) in stock processing procedure be: in ground, garden, select robust growth, without the raw Dabie Mountain of the 2-3 of disease and pest Chinese ilex,As graft stock, rootstock seedling is cut off apart from the above branches and leaves part in 5～8 centimetres, ground, remove the branch below clip, fromClip is downward, with cutting point by long cortex rip cutting one 1～2 centimetre of dark perpendicular mouthful of reaching xylem;
Described step B) in scion processing, choose the current-year branch of the color leaf Chinese ilex of Na in drag-line, the truncation of scion upper cut, lower cutCut sth. askew; Length is 3cm, first processes with high-voltage electrostatic field, and intensity is 50KV/cm, and the time is 30min, then first with plantingThing growth regulator soaks and soaks respectively with DMSO, and described plant growth regulator is: 5 parts of indolebutyric acids, diethyl aminoethyl hexanoate 3Part, 2 parts of gibberellin, KT-301 part, VB11 part, with the dilution ratio preparation plant growth regulating of 150mg: 1LAgent, then soaks respectively branch bottom 15min with described plant growth regulator, 1%DMSO;
Described step C) in the grafting time that adopts be: in general, be suitable in comparatively in good time 2 months of temperature, the last ten-days period;
Described step D) in the engrafting method that adopts be: use the method for scion grafting, and with without dripping film, interface being tightly wound around and being wrapped from bottom to topPrick;
Described step e) in adopt managing after grafting method be: attention moisturizing after grafting completes, prevents mechanical damage; Often note seeingExamine, maintenance, carry out foliage top dressing in peak period, fast-growing phase, growth later stage respectively, the 1st minor peaks phase, spray every 5d blade faceExecute 0.1%-0.3% calcium superphosphate and potassium dihydrogen phosphate, promote seedling root to grow and stem lignifying, the 2nd speedThe raw phase 7-8 month, every 5-7d foliage-spray 0.1%-0.2% urea liquid, promotes seedling growth, the seedling growth later stage, every5-7d foliage-spray 0.3% potassium dihydrogen phosphate or 0.2%-0.3% potassium sulfate solution, promote nursery stock lignifying, and enhancing is survived the winter cold-resistantAbility.
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| CN106070348A (en) * | 2016-06-23 | 2016-11-09 | 广西玉林天地网农业科技有限公司 | A kind of Fructus Citri Sarcodactylis graft union agent and preparation method thereof |
| CN107148860A (en) * | 2017-05-08 | 2017-09-12 | 茂名市绿凰农业发展有限公司 | It is a kind of that the engrafting method of the high-quality and efficient safety in production of crop is promoted based on high-voltage electrostatic field |
| CN108934516B (en) * | 2018-08-13 | 2021-03-16 | 江苏省中国科学院植物研究所 | Later-period management method for improving grafting survival rate of ilex verticillata |
| CN109845502A (en) * | 2018-12-15 | 2019-06-07 | 江苏省中国科学院植物研究所 | A method of stock grafting ilex verticillata is made with Chinese holly |
| CN109548505A (en) * | 2019-01-17 | 2019-04-02 | 曹春花 | The grafting and breeding technique of Magnolia wufengensis |
| CN109566130A (en) * | 2019-01-25 | 2019-04-05 | 曹春花 | A kind of method of scale fast breeding Magnolia wufengensis |
| CN111034484A (en) * | 2019-12-06 | 2020-04-21 | 西安圣华农业科技股份有限公司 | Method for grafting plums on adult peach trees |
| CN116530348A (en) * | 2022-11-24 | 2023-08-04 | 永仁远南农业综合开发有限公司 | Rapid propagation method for female-promoting fruiting of Holly in North America |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569270A (en) * | 2009-06-08 | 2009-11-04 | 江苏省林业科学研究院 | Method for improving graft survival rate of holly |
| CN101692773A (en) * | 2009-10-09 | 2010-04-14 | 张冠一 | Management method of grafted holly |
| CN102007849A (en) * | 2009-09-08 | 2011-04-13 | 杜丽英 | Ilex latifoliae cion conjoined lippedjoint method |
| CN103039275A (en) * | 2012-12-21 | 2013-04-17 | 袁红 | Process for grafting purpus privet by taking ilex latifolia thumb as root stock |
| CN103070027A (en) * | 2013-01-31 | 2013-05-01 | 江西省喜果绿化有限公司 | Method for cultivating cold-resistant tree-shaped fruit ilex |
| CN103070026A (en) * | 2013-01-31 | 2013-05-01 | 江西省喜果绿化有限公司 | Method for rapidly culturing tree-based fruit holly |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100942193B1 (en) * | 2007-11-23 | 2010-02-17 | 대한민국 | Development of New Ornamental Cultivar in Ilex crenata Thunb. var. crenata Golden leaf Using rockwool |
| KR101030517B1 (en) * | 2008-06-30 | 2011-04-26 | 김유진 | Asexual propagation method for Ilex rotunda Thunberg having golden leaf |
| KR101145310B1 (en) * | 2009-11-11 | 2012-06-27 | 대한민국 | Propagation and multi-clones expression technique of Ilex rotunda Thunb by grafting on one stock |
-
2013
- 2013-07-04 CN CN201310277677.5A patent/CN104160878B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569270A (en) * | 2009-06-08 | 2009-11-04 | 江苏省林业科学研究院 | Method for improving graft survival rate of holly |
| CN102007849A (en) * | 2009-09-08 | 2011-04-13 | 杜丽英 | Ilex latifoliae cion conjoined lippedjoint method |
| CN101692773A (en) * | 2009-10-09 | 2010-04-14 | 张冠一 | Management method of grafted holly |
| CN103039275A (en) * | 2012-12-21 | 2013-04-17 | 袁红 | Process for grafting purpus privet by taking ilex latifolia thumb as root stock |
| CN103070027A (en) * | 2013-01-31 | 2013-05-01 | 江西省喜果绿化有限公司 | Method for cultivating cold-resistant tree-shaped fruit ilex |
| CN103070026A (en) * | 2013-01-31 | 2013-05-01 | 江西省喜果绿化有限公司 | Method for rapidly culturing tree-based fruit holly |
Non-Patent Citations (1)
| Title |
|---|
| 彩叶枸骨叶冬青嫁接育苗试验;章建红等;《浙江林业科技》;20080331;第28卷(第2期);第58-60页 * |
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