CN101699958B - Method for cottage propagation of schisandra sphenanthera rehd.et wils - Google Patents
Method for cottage propagation of schisandra sphenanthera rehd.et wils Download PDFInfo
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- CN101699958B CN101699958B CN2009102189318A CN200910218931A CN101699958B CN 101699958 B CN101699958 B CN 101699958B CN 2009102189318 A CN2009102189318 A CN 2009102189318A CN 200910218931 A CN200910218931 A CN 200910218931A CN 101699958 B CN101699958 B CN 101699958B
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Abstract
The invention relates to a method for cottage propagation of schisandra sphenanthera rehd.et wils, which comprises the following steps of: treating cuttings, preparing rooting promoter, preparing seedbeds, and carrying out cottage and matched management. Based on the traditional cottage propagation method, a novel and efficient plant propagating method is formed through adjusting the proportion of the rooting promoter. The method for cottage propagation of schisandra sphenanthera rehd.et wils has convenient production operation as well as fast and stable propagation and can carry out cottage in spring and autumn in one year. The invention can be used for fast propagation of the schisandra sphenanthera rehd.et wils.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to a kind of schisandra chinensis cuttage breeding method.
Background technology
Schisandra chinensis (Schisandra sphenanthera Rehd.et Wils.) is the perennial fallen leaves woody climber of Schisandraceae (Schisandraceae) Schizandra (Schisandra Michx.), its dry mature fruit is called as kadsura longepedunculata, include in one one of Chinese Pharmacopoeia version in 2005, have that convergence is astringent or styptic treatment for spontaneous sweating, an effect of nourishing generate fluid, kidney calming.Be distributed in Shanxi, Shaanxi, Gansu, Shandong, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Henan, Hubei, Hunan, Guizhou, northeast of Yunnan.Further exploitation along with development and national economy and berry purposes, the domestic and international market increases year by year to the demand of Central China Schisandra sphenanthera Rehd.et WilS, but because clear woods fosters and human factor such as long-term predation formula harvesting is destroyed, wild resource significantly reduces, cause schisandra chinensis market supply deficiency, therefore, carrying out large tracts of land artificial cultivation, the quick breeding of research and high yield technology will be one of effective way that solves its shortage of resources.
Production practices show that the development crops produce, and must grasp the seed selection improved seeds and improve two links of culture technique.Only select improved seeds for use, use suitable culture technique, just may produce the product of high yield and high quality, obtain good cultivation effect.The cultivation schisandra chinensis adopts seminal propagation or wild[l Gui Pu to plant more at present.In seminal propagation, the seed dormancy phase reaches for 5～June, and the spring sowing germination rate is low, and individual easily sudden change and generation proterties are degenerated (fruit ear is not of uniform size, the fruit grain is little and rare), and output is lower.And wild[l is the branch that sends from the underground horizontal stem of parent, does not have vertical main root, and transplanting survival rate is low, and a little less than plant drought resisting and the cold resistance, adaptability is relatively poor.
Summary of the invention
Technical problem to be solved by this invention is to overcome the shortcoming of above-mentioned schisandra chinensis breeding, provide a kind of and draw materials conveniently, simple to operate, survival rate is high, two seasons (spring and autumn) produce, cost is lower, be widely used, the schisandra chinensis cuttage breeding method of reliable and stable, easy popularization.
Solving the problems of the technologies described above the technical scheme that is adopted is that it is made up of following step:
1, handles plugged ear
Select 1～2 year living schisandra chinensis branch in spring or autumn, between its morphologic middle part and middle and lower part, cut the schisandra chinensis plugged ear of the long 15～20cm of being with secateurs, the schisandra chinensis plugged ear keeps 3～4 buds, keep 1 leaf near schisandra chinensis plugged ear upper end, the last bud 1cm straight snips of upper cut distance of schisandra chinensis plugged ear, lower cut distance bud 2cm down is cut into level and smooth inclined-plane.
2, preparation root-inducing promoter
The 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 25～300mg
Cobalamin 10～100mg
Iron chloride 5～100mg
Distilled water adds to 1000mL
3, preparing nursery bed
The high 1.2m in seedbed, long 5.0m, wide 1.0m, dark 0.6m, one deck 15～20cm thick cobblestone in shop at the bottom of the bed of seedbed, on cover the thick sandy soil of one deck 45～55cm, sand is 1: 3 with the volume ratio of soil in sandy soil, is that 0.3% liquor potassic permanganate sprinkling sandy soil carry out disinfection with mass concentration, and the potassium permanganate consumption is to soak into sandy soil degree of being, sterilized 2 hours, with the running water clean potassium permanganate of drenching, shakeout, standby.
4, cuttage
The lower cut of schisandra chinensis plugged ear is immersed in the root-inducing promoter of step 2 preparation, handled 6～18 hours, take out, with flushing with clean water 1～2 time, there is not mucus until incision, the direction that schisandra chinensis plugged ear and seedbed is at 45 is inserted in the sandy soil, the length that the schisandra chinensis plugged ear is inserted in the sandy soil is 1/4～1/2 of schisandra chinensis cutting length, seeding row spacing between one a strain schisandra chinensis plugged ear and the adjacent strain schisandra chinensis plugged ear is 3cm * 5cm, press real schisandra chinensis plugged ear sandy soil on every side with hand, at 5m
2The seedbed in to spray mass concentration be the sterilization ling aqueous solution 1.5～2.5L of 5%, spray water is used the plastic membrane sealing seedbed to soak into sandy soil degree of being again, preserves moisture and cultivates.
Above-mentioned sterilization ling is the commodity of market sale, is produced by Tianjin section close europeanized reagent development centre.
5, supporting management
After the cuttage, regularly open the seedbed of sealing every day and ventilated 60～90 minutes, and spray water is to soak into sandy soil degree of being; The last fortnight after the cuttage, every interval 7 days is at 5m
2The seedbed in spray 1.5～2.5L root-inducing promoter, spray 1.5～2.5L mass concentration again and be 5% the sterilization ling aqueous solution, play every interval 7 days the 3rd week at 5m
2The seedbed in to spray 1.5～2.5L mass concentration be 0.1% urea, spray 1.5～2.5L mass concentration again and be 5% the sterilization ling aqueous solution, the temperature maintenance in seedbed is at 15～20 ℃, relative moisture maintains 50%～80%, till root occurring.
In preparation root-inducing promoter step 2 of the present invention, the 1000mL root-inducing promoter is made by following best in quality proportion raw material:
Heteroauxin 100mg
Cobalamin 50mg
Iron chloride 10mg
Distilled water adds to 1000mL.
In cuttage step 4, the lower cut of plugged ear is immersed in the root-inducing promoter of step 2 preparation optimization process 12 hours.In supporting management step 5, after the cuttage, regularly open the seedbed of sealing every day and ventilated 60～90 minutes, and spray water is to soak into sandy soil degree of being; The last fortnight after the cuttage is weekly regularly at 5m
2The seedbed in the best 2L root-inducing promoter that sprays, the best again 2L mass concentration of spraying is 5% the sterilization ling aqueous solution, the 3rd week rose weekly regularly at 5m
2The seedbed in the best 2L mass concentration of spraying be 0.1% aqueous solution of urea, the best again 2L mass concentration of spraying is 5% the sterilization ling aqueous solution, the temperature maintenance in seedbed is at 15～20 ℃, the relative moisture best maintained is 60%, till taking root.
The present invention by adjusting the proportioning of root-inducing promoter, has formed method for propagation efficiently on the basis of traditional cuttage breeding method.Adopt this method to carry out the cottage propagation of schisandra chinensis, have that production operation is convenient, breeding fast and advantage such as stable, in 1 year can spring and autumn carry out two season cottage propagation.Can be used for the quick breeding of schisandra chinensis.
Embodiment
The present invention is described in more detail below in conjunction with embodiment, but the invention is not restricted to these embodiment.
Embodiment 1
1, handles plugged ear
Choose 1～2 year 150 in living schisandra chinensis branch spring and (be divided into three groups, every group 50), between its morphologic middle part and middle and lower part, cut the schisandra chinensis plugged ear of the long 15～20cm of being with secateurs, schisandra chinensis fringe bar keeps 3 buds, keep 1 leaf near schisandra chinensis plugged ear upper end, the last bud 1cm straight snips of upper cut distance of schisandra chinensis plugged ear, lower cut distance bud 2cm down is cut into level and smooth inclined-plane.
2, preparation root-inducing promoter
The 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 100mg
Cobalamin 50mg
Iron chloride 10mg
Distilled water adds to 1000mL
3, preparing nursery bed
The high 1.2m in seedbed, long 5.0m, wide 1.0m, dark 0.6m, one deck 15～20cm thick cobblestone in shop at the bottom of the bed of seedbed, on cover the thick sandy soil of one deck 45～55cm, sandy soil are to be mixed and made at 1: 3 by sand and soil by volume, at 5m
2The seedbed in be that 0.3% potassium permanganate solution carries out disinfection with mass concentration, the potassium permanganate consumption was sterilized 2 hours to soak into sandy soil degree of being, it is clean to drench with running water, shakeouts, standby.
4, cuttage
The lower cut of schisandra chinensis plugged ear is immersed in the root-inducing promoter 12 hours, take out, with flushing with clean water 1～2 time, there is not mucus until incision, the direction that schisandra chinensis plugged ear and seedbed is at 45 is inserted in the sandy soil, the length that the schisandra chinensis plugged ear is inserted in the sandy soil is 1/4～1/2 of schisandra chinensis cutting length, guarantee that 1～2 bud and 1 leaf are exposed in the air, line-spacing between one a strain schisandra chinensis plugged ear and the adjacent strain schisandra chinensis plugged ear is 3cm * 5cm, press real plugged ear sandy soil on every side with hand, at 5m
2The seedbed in to spray mass concentration be 5% sterilization ling aqueous solution 2L, spray water is used the plastic membrane sealing seedbed to soak into sandy soil degree of being again, preserves moisture and cultivates.
5, supporting management
After the cuttage, regularly open the seedbed of sealing every day and ventilated 60～90 minutes, and spray water is to soak into sandy soil degree of being; The last fortnight after the cuttage is weekly regularly at 5m
2The seedbed in spray the 2L root-inducing promoter, spray the 2L mass concentration again and be 5% the sterilization ling aqueous solution, the 3rd week rose weekly regularly at 5m
2The seedbed in to spray the 2L mass concentration be 0.1% aqueous solution of urea, spray the 2L mass concentration again and be 5% the sterilization ling aqueous solution, the temperature maintenance in seedbed is at 15～20 ℃, relative moisture maintains 60%, till taking root.By statistics, the schisandra chinensis plugged ear began to take root in the 45th day, and rooting rate reaches 88.0% in the time of the 50th day.
Embodiment 2
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 25mg
Cobalamin 10mg
Iron chloride 5mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 3
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 300mg
Cobalamin 100mg
Iron chloride 100mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 4
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 25mg
Cobalamin 100mg
Iron chloride 100mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 5
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 300mg
Cobalamin 10mg
Iron chloride 100mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 6
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 300mg
Cobalamin 100mg
Iron chloride 5mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 7
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 300mg
Cobalamin 10mg
Iron chloride 5mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 8
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 25mg
Cobalamin 100mg
Iron chloride 5mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 9
In the preparation root-inducing promoter step 2 of present embodiment, the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 25mg
Cobalamin 10mg
Iron chloride 100mg
Distilled water adds to 1000mL
Other steps are identical with embodiment 1.
Embodiment 10
In the cuttage step 4 of embodiment 1～9, the lower cut of plugged ear is immersed in the root-inducing promoter of step 2 preparation, handled 6 hours, at 5m
2The seedbed in to spray mass concentration be 5% sterilization ling aqueous solution 1.5L, other steps of this step are identical with embodiment 1.
In supporting management step 5, after the cuttage, regularly open the seedbed of sealing every day and ventilated 60～90 minutes, and spray water is to soak into sandy soil degree of being; The last fortnight after the cuttage, weekly at interval 7 days at 5m
2The seedbed in spray the 1.5L root-inducing promoter, spray the 1.5L mass concentration again and be 5% the sterilization ling aqueous solution, play weekly at interval 7 days the 3rd week at 5m
2The seedbed in to spray the 1.5L mass concentration be 0.1% urea, spray the 1.5L mass concentration again and be 5% the sterilization ling aqueous solution, the temperature maintenance in seedbed is at 15～20 ℃, relative moisture maintains 50%, till root occurring.
Other steps are identical with respective embodiments.
Embodiment 11
In the cuttage step 4 of embodiment 1～9, the lower cut of plugged ear is immersed in the root-inducing promoter of step 2 preparation, handled 18 hours, at 5m
2The seedbed in to spray mass concentration be 5% sterilization ling aqueous solution 2.5L, other steps of this step are identical with embodiment 1.
In supporting management step 5, after the cuttage, regularly open the seedbed of sealing every day and ventilated 60～90 minutes, and spray water is to soak into sandy soil degree of being; The last fortnight after the cuttage, weekly at interval 7 days at 5m
2The seedbed in spray the 2.5L root-inducing promoter, spray the 2.5L mass concentration again and be 5% the sterilization ling aqueous solution, play weekly at interval 7 days the 3rd week at 5m
2The seedbed in to spray the 2.5L mass concentration be 0.1% urea, spray the 2.5L mass concentration again and be 5% the sterilization ling aqueous solution, the temperature maintenance in seedbed is at 15～20 ℃, relative moisture maintains 80%, till root occurring.
Other steps are identical with respective embodiments.
Embodiment 12
In the processing plugged ear step 1 of embodiment 1～11, choose 1～2 year 150 in living schisandra chinensis branch (being divided into three groups, 50 every group) autumn, other steps of this step are identical with embodiment 1.Other steps are identical with respective embodiments.
For the optimization procedure of definite the inventive method and the optimum material proportion of used root-inducing promoter, the inventor has carried out a large amount of laboratories and field trial, and various test situation are as follows:
Test material: 1～2 year living branch of schisandra chinensis (Schisandra sphenanthera Rehd.et Wils.), phoenix town, Zuoshui County, Shaanxi is picked up from year March in October, 2006～2009, and it is standby to take back the laboratory through the water conservation processing.
Plugged ear is prepared: the group test of completely random district is adopted in test.Handle 50 schisandra chinensis plugged ears, repeat 3 times for every group.Select 1～2 year living schisandra chinensis branch, between its morphologic middle part and middle and lower part, block to growing the schisandra chinensis plugged ear of 15～20cm with secateurs, the schisandra chinensis plugged ear keeps 3～4 buds, and keep near 1 leaf on the schisandra chinensis plugged ear, the last bud 1cm of the upper cut distance place straight snips of schisandra chinensis plugged ear, lower cut distance bud 2cm down is cut into level and smooth inclined-plane.
1, the raw material of determining root-inducing promoter is formed and proportioning
The schisandra chinensis plugged ear that the plugged ear of learning from else's experience is prepared, with concentration is that heteroauxin (IAA), indolebutyric acid (IBA), root-inducing powder ABT1 (buying from Shaanxi State Forestry Administration, P.R. China plant protection unit), the root-inducing powder GGR6 (buying from Shaanxi State Forestry Administration, P.R. China plant protection unit) of 100mg/L handled schisandra chinensis plugged ear base portion respectively 12 hours, is treated to contrast with clear water.Other steps are identical with embodiment 1, and experimental result sees Table 1.
The various raw materials of table 1 are to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage effect
Annotate: employing LSD method is carried out significance analysis, and same row contain lowercase person inequality and are significant difference P＜0.05, and same row contain different capitalization persons and are the extremely remarkable P of difference＜0.01, down together.
By table 1 as seen, adopting concentration is the heteroauxin of 100mg/L, indolebutyric acid, root-inducing powder ABT1, the schisandra chinensis plugged ear that root-inducing powder GGR6 handles is compared with the clear water contrast, the callus apparition, the wound healing phase, take root the phase, rooting rate, survive the seedling rooting number utmost point significant difference (P＜0.01) is arranged, there were significant differences (P＜0.05) to survive shoot root length, the callus of schisandra chinensis plugged ear occurs early, wound healing is fast, take root the phase in advance, the rooting rate height, it is many to survive the seedling rooting number, obviously be better than control group, show that the schisandra chinensis plugged ear is through heteroauxin, indolebutyric acid, root-inducing powder ABT1, root-inducing powder GGR6 immersion treatment can obviously promote its cuttage root-taking.
Wherein, the seedling rooting number that survives that heteroauxin, soaking with indole butyric acid are handled does not have significant difference, but the utmost point is significantly higher than root-inducing powder ABT1, root-inducing powder GGR6 (P＜0.01).The rooting rate of heteroauxin immersion treatment, survive the long utmost point of shoot root and be better than other processing (P＜0.01) significantly, rooting rate improves 6.87%, 14.58%, 15.42% than indolebutyric acid, root-inducing powder ABT1, root-inducing powder GGR6 respectively, and it is long respectively than indolebutyric acid, root-inducing powder ABT1, root-inducing powder GGR6 growth 0.70cm, 0.96cm, 0.95cm to survive shoot root.So test and Selection heteroauxin of the present invention is that optimum plant hormone is used for the schisandra chinensis cuttage.
2, the heteroauxin immersion treatment of variable concentrations is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
The schisandra chinensis plugged ear that the plugged ear of learning from else's experience is prepared, be the heteroauxin immersion treatment schisandra chinensis plugged ear base portion 12 hours of 25mg/L, 50mg/L, 100mg/L, 150mg/L, 200mg/L, 250mg/L, 300mg/L with concentration respectively, be treated to contrast with clear water.Other steps are identical with embodiment 1.Experimental result sees Table 2.
The heteroauxin immersion treatment of table 2 variable concentrations is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
By table 2 as seen, the heteroauxin of variable concentrations is compared with clear water contrast immersion treatment schisandra chinensis plugged ear, callus apparition, wound healing phase, the phase of taking root, rooting rate, survive the seedling rooting number, all there were significant differences (P＜0.05) to survive the long every index of shoot root, when concentration is 25～300mg/L, rooting rate is higher, and wherein concentration is 100mg/L rooting rate the best.
3, different types of vitamin immersion treatment is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
The schisandra chinensis plugged ear that the plugged ear of learning from else's experience is prepared, adding vitamin A, vitamin B6, cobalamin, vitamin C, vitamin D, vitamin E immersion treatment schisandra chinensis plugged ear base portion 12 hours that concentration is 25mg/L in concentration is the heteroauxin of 100mg/L respectively, is that the heteroauxin of 100mg/L is treated to contrast with concentration.Other steps are identical with embodiment 1.Experimental result sees Table 3.
The different types of vitamin immersion treatment of table 3 is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
By table 3 as seen, in concentration is to add the cobalamin processed group in the heteroauxin of 100mg/L to compare with other processed group, callus apparition, wound healing phase, the phase of taking root, survive long 4 indexs of shoot root and do not have significant difference, but its rooting rate and survive several two indexs of seedling rooting and extremely significantly be better than other groups (P＜0.01).The present invention is chosen in and adds the root-growing agent of cobalamin as the schisandra chinensis cuttage in the heteroauxin.
4, the cobalamin immersion treatment of variable concentrations is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
The schisandra chinensis plugged ear that the plugged ear of learning from else's experience is prepared, in being the heteroauxin of 100mg/L, concentration adds the cobalamin that mass concentration is 10mg/L, 25mg/L, 50mg/L, 75mg/L, 100mg/L respectively, immersion treatment schisandra chinensis plugged ear base portion is 12 hours respectively, is that the heteroauxin of 100mg/L is treated to contrast with concentration.Other steps are identical with embodiment 1.
Experimental result sees Table 4.
The cobalamin immersion treatment of table 4 variable concentrations is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
By table 4 as seen, when mass concentration is 10～100mg/L, rooting rate difference is obvious between each processed group, especially when the concentration of cobalamin is 50mg/L, rooting rate reaches 64.67%, the utmost point is significantly higher than other each processed group (P＜0.01), and it is 10～100mg/L that the present invention selects the mass concentration of cobalamin, and optium concentration is 50mg/L.
5, different types of micro-immersion treatment is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
The schisandra chinensis plugged ear that the plugged ear of learning from else's experience is prepared, in mass concentration is the heteroauxin of 100mg/L, add zinc sulphate, sodium hydrogen phosphate, magnesium chloride, iron chloride, copper sulphate immersion treatment schisandra chinensis plugged ear base portion 12 hours that mass concentration is 10mg/L respectively, with mass concentration be that the heteroauxin of 100mg/L is treated to contrast.Other steps are identical with embodiment 1.
Experimental result sees Table 5.
The different types of micro-immersion treatment of table 5 is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
By table 5 as seen, add the iron chloride processed group and compares, rooting rate, survive the seedling rooting numerical table and reveal utmost point significant difference (P＜0.01), compare the equal no significant difference of every index and add other micro-processed group with the heteroauxin control group with the heteroauxin contrast.The present invention is chosen in and adds the root-growing agent of iron chloride as the schisandra chinensis cuttage in the heteroauxin.
6, the iron chloride immersion treatment of variable concentrations is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
The schisandra chinensis plugged ear that the plugged ear of learning from else's experience is prepared, in being the heteroauxin of 100mg/L, mass concentration adds the iron chloride that mass concentration is 5mg/L, 10mg/L, 30mg/L, 50mg/L respectively, immersion treatment schisandra chinensis plugged ear base portion is 12 hours respectively, is treated to contrast with the mass concentration for the 100mg/L heteroauxin.Other steps are identical with embodiment 1.Experimental result sees Table 6.
The iron chloride immersion treatment of table 6 different quality concentration is to the influence of Central China Schisandra sphenanthera Rehd.et WilS cuttage
By table 6 as seen, when the mass concentration of iron chloride was 5～50mg/L, the iron chloride processed group was compared with the heteroauxin control group, and rooting rate shows utmost point significant difference (P＜0.01), and wherein mass concentration is that 10mg/L rooting rate the best is 61.33%.
7, four kinds of raw material soaking are handled the influence to Central China Schisandra sphenanthera Rehd.et WilS cuttage
The schisandra chinensis plugged ear that the plugged ear of learning from else's experience is prepared, with mass concentration is the heteroauxin of 100mg/L, the cobalamin that mass concentration is 50mg/L, four kinds of comprehensive immersion treatment schisandra chinensis of raw material of iron chloride, distilled water plugged ear that mass concentration is 10mg/L, with the mass concentration be the heteroauxin of 100mg/L and cobalamin that concentration is 50mg/L comprehensive in contrast 1 or with concentration be the iron chloride comprehensively in contrast 2 of 10mg/L.Other steps are identical with embodiment 1.Experimental result sees Table 7.
Four kinds of raw material soaking of table 7 are handled the influence to Central China Schisandra sphenanthera Rehd.et WilS cuttage
By table 7 as seen, integrated treatment is handled with contrast 2 with contrast 1 and is compared, callus healing stage, wound healing phase, survive that shoot root is long all not to have a significant difference, but the phase of taking root, rooting rate, survive seedling rooting and count significant difference (P＜0.05), show as morning phase of taking root, rooting rate height, to survive the seedling rooting number many, wherein rooting rate improves 24.00%, 27.34% respectively, and surviving the seedling rooting number increases by 1.04 and 1.67 respectively.Therefore, integrated treatment is the most obvious to Central China Schisandra sphenanthera Rehd.et WilS plugged ear cuttage root-taking facilitation.
In sum, optimal selection of the present invention is mixed with the cottage propagation that root-inducing promoter is used for schisandra chinensis with heteroauxin, cobalamin, iron chloride, distilled water, and wherein the best in quality concentration of heteroauxin, cobalamin, iron chloride is respectively 100mg/L, 50mg/L, 10mg/L.
Claims (3)
1. schisandra chinensis cuttage breeding method is characterized in that it is made up of following step:
(1) handles plugged ear
Select 1～2 year living schisandra chinensis branch in spring or autumn, between its morphologic middle part and middle and lower part, cut the schisandra chinensis plugged ear of the long 15～20cm of being with secateurs, the schisandra chinensis plugged ear keeps 3～4 buds, keep 1 leaf near schisandra chinensis plugged ear upper end, the last bud 1cm straight snips of upper cut distance of schisandra chinensis plugged ear, lower cut distance bud 2cm down is cut into level and smooth inclined-plane;
(2) preparation root-inducing promoter
The 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 25～300mg
Cobalamin 10～100mg
Iron chloride 5～100mg
Distilled water adds to 1000mL;
(3) preparing nursery bed
The high 1.2m in seedbed, long 5.0m, wide 1.0m, dark 0.6m, one deck 15～20cm thick cobblestone in shop at the bottom of the bed of seedbed, on cover the thick sandy soil of one deck 45～55cm, sand is 1: 3 with the volume ratio of soil in sandy soil, is that 0.3% liquor potassic permanganate sprinkling sandy soil carry out disinfection with mass concentration, and the potassium permanganate consumption is to soak into sandy soil degree of being, sterilized 2 hours, with the running water clean potassium permanganate of drenching, shakeout, standby;
(4) cuttage
The lower cut of schisandra chinensis plugged ear is immersed in the root-inducing promoter of step (2) preparation, handled 6～18 hours, take out, with flushing with clean water 1～2 time, there is not mucus until incision, the direction that schisandra chinensis plugged ear and seedbed is at 45 is inserted in the sandy soil, the length that the schisandra chinensis plugged ear is inserted in the sandy soil is 1/4～1/2 of schisandra chinensis cutting length, seeding row spacing between one a strain schisandra chinensis plugged ear and the adjacent strain schisandra chinensis plugged ear is 3cm * 5cm, press real schisandra chinensis plugged ear sandy soil on every side with hand, at 5m
2The seedbed in to spray mass concentration be the sterilization ling aqueous solution 1.5～2.5L of 5%, spray water is used the plastic membrane sealing seedbed to soak into sandy soil degree of being again, preserves moisture and cultivates;
(5) supporting management
After the cuttage, regularly open the seedbed of sealing every day and ventilated 60～90 minutes, and spray water is to soak into sandy soil degree of being; The last fortnight after the cuttage, every interval 7 days is at 5m
2The seedbed in spray 1.5～2.5L root-inducing promoter, spray 1.5～2.5L mass concentration again and be 5% the sterilization ling aqueous solution, play every interval 7 days the 3rd week at 5m
2The seedbed in to spray 1.5～2.5L mass concentration be 0.1% urea, spray 1.5～2.5L mass concentration again and be 5% the sterilization ling aqueous solution, the temperature maintenance in seedbed is at 15～20 ℃, relative moisture maintains 50%～80%, till root occurring.
2. according to the described schisandra chinensis cuttage breeding method of claim 1, it is characterized in that: in preparation root-inducing promoter step (2), the 1000mL root-inducing promoter is made by following quality proportion raw material:
Heteroauxin 100mg
Cobalamin 50mg
Iron chloride 10mg
Distilled water adds to 1000mL.
3. according to the described schisandra chinensis cuttage breeding method of claim 1, it is characterized in that: in cuttage step (4), the lower cut of plugged ear is immersed in the root-inducing promoter of step (2) preparation, handled 12 hours; The last fortnight after the cuttage is weekly regularly at 5m
2The seedbed in spray the 2L root-inducing promoter, spray the 2L mass concentration again and be 5% the sterilization ling aqueous solution, the 3rd week rose weekly regularly at 5m
2The seedbed in to spray the 2L mass concentration be 0.1% aqueous solution of urea, spray the 2L mass concentration again and be 5% the sterilization ling aqueous solution, relative moisture maintains 60%, till taking root.
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| CN103609594B (en) * | 2013-10-23 | 2016-02-10 | 青岛文创科技有限公司 | A kind of turfgrass root-growing agent |
| CN103749137B (en) * | 2014-01-16 | 2016-01-20 | 四川农业大学 | A kind of cuttage and seedling culture method of Malus toringoides Hughes |
| CN103918438B (en) * | 2014-03-31 | 2015-11-18 | 临沧双滢生物科技有限公司 | The cottage method of a kind of Schisandraceae Plant Kadsura interior or Kadsura heteroclita |
| CN105393763A (en) * | 2015-11-18 | 2016-03-16 | 范健身 | Method for planting Sargentodoxa cuneata |
| CN114521401A (en) * | 2022-03-01 | 2022-05-24 | 吉林农业大学 | Method for raising schisandra chinensis seedlings by cutting |
| CN115088490B (en) * | 2022-07-11 | 2024-04-05 | 陕西省西安植物园 | Cuttage method for promoting schisandra chinensis to rapidly root |
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Non-Patent Citations (3)
| Title |
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| 柳丽.北五味子扦插繁殖试验.《特产研究》.1991,(第02期), * |
| 梁爽等.五味子绿枝扦插繁殖的影响因素研究.《安徽农业科学》.2007,(第06期), * |
| 陈建军等.北五味子硬枝扦插育苗研究初报.《中药材》.1991,(第01期), * |
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