CN107047258B - Special culture medium for dendrobium nobile lindl - Google Patents
Special culture medium for dendrobium nobile lindl Download PDFInfo
- Publication number
- CN107047258B CN107047258B CN201710258252.8A CN201710258252A CN107047258B CN 107047258 B CN107047258 B CN 107047258B CN 201710258252 A CN201710258252 A CN 201710258252A CN 107047258 B CN107047258 B CN 107047258B
- Authority
- CN
- China
- Prior art keywords
- dendrobium
- microbial inoculum
- composite microbial
- amino acid
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000001963 growth medium Substances 0.000 title claims abstract description 9
- 240000004638 Dendrobium nobile Species 0.000 title claims description 8
- 239000000758 substrate Substances 0.000 claims abstract description 36
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 27
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 27
- 241001330002 Bambuseae Species 0.000 claims abstract description 27
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 27
- 239000011425 bamboo Substances 0.000 claims abstract description 27
- 241000196324 Embryophyta Species 0.000 claims abstract description 22
- 150000001413 amino acids Chemical class 0.000 claims abstract description 21
- 239000002131 composite material Substances 0.000 claims abstract description 21
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 19
- 239000002609 medium Substances 0.000 claims abstract description 18
- 239000002245 particle Substances 0.000 claims abstract description 16
- 239000011573 trace mineral Substances 0.000 claims abstract description 15
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 15
- 239000003610 charcoal Substances 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 12
- 235000010204 pine bark Nutrition 0.000 claims abstract description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 235000013311 vegetables Nutrition 0.000 claims abstract description 11
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 10
- 230000004151 fermentation Effects 0.000 claims abstract description 10
- 239000004021 humic acid Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 241000361919 Metaphire sieboldi Species 0.000 claims abstract description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 6
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 6
- 229910052796 boron Inorganic materials 0.000 claims description 6
- 229910052791 calcium Inorganic materials 0.000 claims description 6
- 239000011575 calcium Substances 0.000 claims description 6
- 229910052742 iron Inorganic materials 0.000 claims description 6
- 229910052749 magnesium Inorganic materials 0.000 claims description 6
- 239000011777 magnesium Substances 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 239000011701 zinc Substances 0.000 claims description 6
- 229910052725 zinc Inorganic materials 0.000 claims description 6
- 244000075850 Avena orientalis Species 0.000 claims description 5
- 235000007319 Avena orientalis Nutrition 0.000 claims description 5
- 235000007558 Avena sp Nutrition 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 241000194105 Paenibacillus polymyxa Species 0.000 claims description 5
- 241001465752 Purpureocillium lilacinum Species 0.000 claims description 5
- 241000223261 Trichoderma viride Species 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 229910052802 copper Inorganic materials 0.000 claims description 5
- 239000010949 copper Substances 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 5
- 240000002791 Brassica napus Species 0.000 claims description 4
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 4
- 241000209140 Triticum Species 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 206010021143 Hypoxia Diseases 0.000 claims description 3
- 238000000197 pyrolysis Methods 0.000 claims description 3
- 241001523681 Dendrobium Species 0.000 abstract description 37
- 239000011159 matrix material Substances 0.000 abstract description 16
- 201000010099 disease Diseases 0.000 abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 244000000010 microbial pathogen Species 0.000 abstract description 5
- 230000002401 inhibitory effect Effects 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 230000035699 permeability Effects 0.000 abstract description 3
- 238000005192 partition Methods 0.000 abstract 1
- 239000010410 layer Substances 0.000 description 30
- 238000011282 treatment Methods 0.000 description 25
- 230000000694 effects Effects 0.000 description 9
- 230000012010 growth Effects 0.000 description 9
- 241001076416 Dendrobium tosaense Species 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 244000052616 bacterial pathogen Species 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000006806 disease prevention Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 239000010438 granite Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 235000021048 nutrient requirements Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 206010037660 Pyrexia Diseases 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 238000013316 zoning Methods 0.000 description 2
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention provides a special culture medium for dendrobium, which comprises an upper layer of medium and a lower layer of medium; the upper layer comprises the following raw materials in percentage by weight: 40-50% of pine bark, 20-30% of bamboo charcoal particles, 20-25% of yeast fermentation material and 5-10% of composite microbial inoculum; the lower layer comprises the following raw materials in percentage by weight: 20-30% of bamboo powder, 20-30% of humic acid, 10-20% of plant source amino acid, 10-15% of vegetable cake, 10-15% of earthworm excrement, 3-5% of medium trace elements and 5-10% of composite microbial inoculum. The substrate provided by the invention has the characteristic of functional partition, and the upper substrate has the characteristics of good air permeability, strong water holding capacity, multiple mechanisms for inhibiting the propagation of pathogenic microorganisms and the like; the lower-layer matrix can meet the long-acting nutritional requirement of the dendrobium in the later period and can prevent the occurrence of diseases from the root.
Description
Technical Field
The invention relates to the technical field of culture substrates, in particular to a special culture substrate for dendrobium.
Background
Dendrobium officinale is a traditional rare Chinese medicinal material in China, has the effects of nourishing yin and clearing heat, tonifying stomach and promoting fluid production, moistening lung and relieving cough and the like, and is commonly used for various diseases such as fluid impairment due to fever, thirst, asthenic fever after illness and the like. Due to forest ecological destruction and excessive exploitation of resources, wild resources are exhausted, and the state department ranks them as national key protection plants in 1987. In order to realize sustainable utilization of dendrobium officinale resources, researchers began to research artificial cultivation techniques since the 70 s of the 20 th century, but key artificial breeding techniques such as seed production, tissue culture and facility cultivation were broken through until the new century, and the artificial cultivation techniques are rapidly popularized and applied. Meanwhile, the research on the medicinal efficacy and value of the dendrobium officinale is also deepened, the health care function of the dendrobium officinale is accepted by more and more people, the market demand of the dendrobium officinale is increased, and further, an industrial chain from planting production and processing to selling of the dendrobium officinale is formed.
The dendrobium planting mode can be seen in two categories of open-air cultivation and facility cultivation at present, wherein the open-air cultivation comprises the modes of stone pasting cultivation, tree pasting cultivation, stone wall cultivation, rock wall cultivation, brick wall cultivation and the like; the facility cultivation includes potting, field cultivation, bed cultivation (overhead seedbed cultivation), multi-layer cultivation, and the like. The planting in a bed planting (overhead seedbed planting) mode is the mainstream mode of planting the dendrobium officinale at present, and more than 90 percent of newly developed planting areas in recent years are planted by using the planting mode. However, the dendrobium nobile cultivation method has the problems of restricting the sustainable development of the industry and the like. The quality and the yield of the dendrobium are greatly influenced by the problems of insufficient nutrients, low water content of the substrate, frequent occurrence of diseases and the like. Once nutrition cannot be kept up or diseases occur, the treatment is carried out, so that the labor cost is increased, the improvement effect is not obvious, and therefore, the development of a substrate which is disease and pest resistant and can continuously provide nutrients is necessary in the field of dendrobium facility cultivation.
Disclosure of Invention
The invention provides a special culture medium for dendrobium, which solves the problems of insufficient nutrients, low water content of the medium and frequent occurrence of diseases in the existing facility culture.
The technical scheme of the invention is realized as follows:
a special culture medium for herba Dendrobii comprises,
two layers of substrates, an upper layer and a lower layer;
the upper layer comprises the following raw materials in percentage by weight: 40-50% of pine bark, 20-30% of bamboo charcoal particles, 20-25% of yeast fermentation material and 5-10% of composite microbial inoculum;
the lower layer comprises the following raw materials in percentage by weight: 20-30% of bamboo powder, 20-30% of humic acid, 10-20% of plant source amino acid, 10-15% of vegetable cake, 10-15% of earthworm excrement, 3-5% of medium trace elements and 5-10% of composite microbial inoculum.
As a preferable technical scheme, the bamboo charcoal particles are solid substances generated by pyrolysis of bamboo at the high temperature of 500-600 ℃ under the condition of oxygen deficiency, and the particle size of the solid substances is 2-5 mm.
As a preferred technical scheme, the compound microbial inoculum is a mixture of bacillus subtilis, paenibacillus polymyxa, trichoderma viride and paecilomyces lilacinus.
As a preferred technical scheme, the plant source amino acid is amino acid obtained by fermenting one or more of soybean, wheat, oat and corn.
As a preferred technical scheme, the vegetable cake is rapeseed cake.
As a preferred technical scheme, the medium trace elements are a mixture of several of calcium, magnesium, zinc, manganese, boron, copper and iron.
As a preferred technical scheme, the upper layer comprises the following raw materials in percentage by weight: 45% of pine bark, 25% of bamboo charcoal particles, 23% of yeast fermentation material and 7% of composite microbial inoculum.
As a preferred technical scheme, the lower layer comprises the following raw materials in percentage by weight: 25% of bamboo powder, 25% of humic acid, 10% of plant source amino acid, 10% of vegetable cake, 15% of earthworm excrement, 5% of medium trace elements and 10% of composite microbial inoculum.
Preferably, the lower layer is twice as thick as the upper layer.
Preferably, the substrate is applied to facility cultivation.
Advantageous effects
The substrate provided by the invention has the characteristic of functional zoning, the upper substrate has the characteristics of good air permeability, strong water holding capacity, multiple mechanisms for inhibiting the propagation of pathogenic microorganisms and the like, and the rooting and field planting of the dendrobium at the early stage are utilized; the lower-layer matrix has the characteristics of rich nutrient species, slow release of nutrients, further disease prevention and the like, and can meet the long-acting nutrient requirement of the dendrobium at the later stage and prevent the disease from occurring fundamentally. The substrate is suitable for various cultivation modes under facility cultivation conditions, can be used for potted plants, bed cultivation and multilayer cultivation, and has a wide application range.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The humic acid of the above examples 1 to 3 is a kind of organic matter including remains of animal things which are decomposed and transformed by microorganisms and then subjected to a series of chemical processes and accumulated. The yeast fermentation material is leftover after yeast is extracted in the yeast fermentation industry. The bamboo powder is obtained by crushing leftover materials after processing bamboo products. The bamboo charcoal particles are solid substances generated by pyrolysis of bamboo at 500-600 ℃ under the condition of oxygen deficiency, and the particle size of the bamboo charcoal particles is 2-5 mm. The composite microbial inoculum is a mixture of bacillus subtilis, paenibacillus polymyxa, trichoderma viride and paecilomyces lilacinus. The plant source amino acid is amino acid obtained by fermenting one or more of soybean, wheat, oat and corn. The vegetable cake is rapeseed cake. The medium trace elements are mixture of calcium, magnesium, zinc, manganese, boron, copper and iron.
The substrate is divided into an upper layer and a lower layer, the thickness of the lower layer is twice of that of the upper layer, the upper layer is mainly made of pine bark, bamboo charcoal and other breathable substances, the water holding capacity of the substrate can be improved, meanwhile, the added composite microbial agent is formed by compounding various microorganisms, the bacillus subtilis and the bacillus polymyxa inhibit the propagation of pathogenic microorganisms through antibiotic and nutrition competition ways, the mycelia of trichoderma viride and paecilomyces lilacinus grow rapidly, a biofilm is easily formed at the root of a crop, the position is occupied, protection is formed, and the invasion of the pathogenic microorganisms is prevented. The lower layer comprises organic substances such as humic acid, amino acid, wormcast, vegetable cake and the like, and also contains a small amount of medium trace elements, so that comprehensive nutrient elements are provided, and the requirements of the later-stage vegetative growth and reproductive growth of the dendrobium on nutrition are met. The substrate provided by the invention has the characteristic of functional zoning, the upper substrate has the characteristics of good air permeability, strong water holding capacity, multiple mechanisms for inhibiting the propagation of pathogenic microorganisms and the like, and the rooting and field planting of the dendrobium at the early stage are utilized; the lower-layer matrix has the characteristics of rich nutrient species, slow release of nutrients, further disease prevention and the like, and can meet the long-acting nutrient requirement of the dendrobium at the later stage and prevent the disease from occurring fundamentally. The substrate is suitable for various cultivation modes under facility cultivation conditions, can be used for potted plants, bed cultivation and multilayer cultivation, and has a wide application range.
Example 1
The special matrix for the dendrobium comprises the following components in percentage by weight:
and (3) upper layer:
40% of pine bark, 30% of bamboo charcoal particles, 20% of yeast fermentation material and 10% of composite microbial inoculum.
The lower layer:
20% of bamboo powder, 30% of humic acid, 15% of plant source amino acid powder, 15% of vegetable cake, 10% of earthworm excrement, 3% of medium trace elements and 7% of composite microbial inoculum; wherein the plant source amino acid is amino acid obtained by fermenting soybean, oat and corn; the medium trace elements are the mixture of calcium, magnesium, zinc, manganese, boron and iron.
Example 2
The special matrix for the dendrobium comprises the following components in percentage by weight:
and (3) upper layer:
45% of pine bark, 25% of bamboo charcoal particles, 23% of yeast fermentation material and 7% of composite microbial inoculum.
The lower layer:
25% of bamboo powder, 25% of humic acid, 10% of plant source amino acid powder, 10% of vegetable cake, 15% of earthworm excrement, 5% of medium trace elements and 10% of composite microbial inoculum; wherein the plant source amino acid is amino acid obtained by fermenting soybean; the medium trace elements are the mixture of calcium, magnesium, zinc, manganese, boron, copper and iron.
Example 3
The special matrix for the dendrobium comprises the following components in percentage by weight:
and (3) upper layer:
50% of pine bark, 20% of bamboo charcoal particles, 25% of yeast fermentation material and 5% of composite microbial inoculum.
The lower layer:
30% of bamboo powder, 20% of humic acid, 20% of plant source amino acid powder, 10% of rapeseed cakes, 10% of earthworm excrement, 5% of medium trace elements and 5% of composite microbial inoculum; amino acid obtained by fermenting plant source amino acid powder wheat, oat and corn; the medium trace elements are mixture of calcium, magnesium, zinc, boron, copper and iron.
Effect verification
According to the special substrate for dendrobium obtained in each of the embodiments 1, 2 and 3, a plot experiment is performed in a facility agricultural district of grand bridge modern park in Shanghai city.
The test results are as follows:
test site: in facility agriculture area of grand bridge modern park in Shanghai city
Test time: 2016 (3/month and 5/year) -2017 (1/month and 31/day)
Test materials:
domesticated dendrobium officinale tissue culture seedlings which grow consistently, are robust and have no diseases;
a conventionally used commercially available dendrobe substrate (a mixture of pine bark and granite in a ratio of 4: 1), a dendrobe substrate obtained according to the present invention;
test design and method:
different treatments are set according to different settings of the dendrobium substrates to be tested,
treatment 1: mixing pine bark and granite according to a ratio of 4: 1;
and (3) treatment 2: a substrate formulated according to example 1;
and (3) treatment: a substrate formulated according to example 2;
and (4) treatment: a substrate formulated according to example 3;
the test arrangement was carried out in a standardized greenhouse, transplanting was carried out in 2016, 3, 5 months, and transplanting was carried out on seedbeds, each seedbed being treated for one plot. 0.8m above the ground of the seedbed, 2.5m long and 1.2m wide of the seedbed, 15cm of total thickness of the substrate, 10cm of lower layer and 5cm of upper layer, selecting disease-free, robust and uniform-size seedlings for field planting, planting the treated seedlings on the substrate according to the specification of 3 plants per cluster, 10cm of plant spacing and 13 cm of row spacing, leaving a certain gap around the seedbed, planting 25 × 8 in each cell together into 100 clusters, preferably completely covering the roots of the substrate during field planting, and watering enough root fixing water. Routine inspection and management work such as spraying and watering, ventilation, temperature and humidity is carried out daily, all treatment is kept consistent, and no fertilizer is applied in the whole planting period. And (3) determining fresh weight, bud number, stem thickness, plant height and other items of a single cluster in 11 months in 2016, wherein the bud number is determined by the total bud number germinated at the base of each cluster, the plant height is determined by the highest stem in the cluster, and the stem thickness is determined by the maximum diameter of the highest stem. The dendrobium anthrax pathogenic bacteria diluent (1:1000) is poured on the dendrobium matrix at the end of 12 months in 2016, and the incidence rate is observed in 1 month, 15 days and 30 days respectively.
Results and analysis
The effect of 4 different treatment substrates on the growth of dendrobe is shown in table 1. The test result shows that: the fertilizer is not applied in the planting period, the growth condition of the dendrobium grown by using the substrate is basically not influenced, the planting rate of seedlings in the early period is higher, the phenomena of dead seedlings and burnt seedlings are basically avoided, the survival rate reaches 98 percent, the leaves of the dendrobium in the whole growth period are dark green, the leaves are wide and thick, new branches continuously sprout in the growth process, the number of sprouts of a single cluster is about 20 generally, and the height and the stem thickness of the dendrobium continuously increase along with the growth. The matrix prepared by using the pine bark and the granite obviously shows malnutrition, the number of single-cluster buds is less, the plant growth is short, and the difference between the plant height and the stem thickness is obvious compared with the treatment 2, the treatment 3 and the treatment 4. In addition, in the planting process, once the temperature in the greenhouse is continuously raised, withered seedlings of the dendrobium are easy to appear, and the dendrobium is recovered after watering, which shows that the continuous water-holding capacity of the matrix is poor. Particularly, from the fresh weight of a single bundle, the fresh weight of the dendrobium cultivated by using the substrate of the invention is 2 times of that of 1 treatment, and the fresh weight of the dendrobium cultivated by using the substrate of the invention is 2 times of that of the dendrobium cultivated by using the substrate of the invention, and the fresh weight of the dendrobium cultivated by using the substrate of the invention is different from the fresh weight of the dendrobium cultivated by using the substrate of. In conclusion, when the substrate disclosed by the invention is used for cultivating the dendrobium, the nutrient requirement for the growth of the dendrobium can be met without additional fertilizer in the growth period, and the substrate has better water holding capacity, so that the dendrobium can be kept in a dark green state all the time.
TABLE 1 Effect of different treatments on the growth of Dendrobium nobile
The results of the effects of different treatments on the incidence rate of the anthracnose of the dendrobium are shown in table 2, and in order to more intuitively and rapidly see the differences among different treatments, the surface layer of the matrix is directly sprayed with the pathogenic bacteria diluent of the anthracnose of the dendrobium in the later period of the experiment so as to observe the disease prevention capability of different matrixes on the anthracnose of the dendrobium. The results show that: after spraying pathogenic bacteria liquid for half a month, the morbidity of the treatment 1 reaches more than half, the morbidity of the treatment after one month basically reaches 70%, the morbidity of the treatment using the matrix is controlled within 10%, the matrix of the invention is added with composite microbial agents on the upper layer and the lower layer of the matrix, the composite microbial agents are mixtures of bacillus subtilis, paenibacillus polymyxa, trichoderma viride and paecilomyces lilacinus, and the effect of inhibiting the propagation and invasion of pathogenic bacteria is achieved through the cooperation of multiple antibacterial mechanisms. The upper layer matrix and the lower layer matrix are respectively provided with a defense line, once the matrix is invaded by pathogenic bacteria, a defense mechanism is started, the pathogenic bacteria are inhibited in the whole process, and the morbidity of the dendrobium is effectively controlled.
TABLE 2 Effect of different treatments on the incidence of anthracnose of Dendrobium
| Treatment of | Treatment of incidence after half a month% | Incidence/% after one month of treatment |
| Process 1 | 50.5 | 68.5 |
| Treatment 2 | 5.3 | 7.6 |
| Treatment 3 | 6.2 | 8.5 |
| Treatment 4 | 7.5 | 9.8 |
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (4)
1. A special culture medium for dendrobium nobile lindl is characterized by comprising,
two layers of substrates, an upper layer and a lower layer;
the upper layer comprises the following raw materials in percentage by weight: 40-50% of pine bark, 20-30% of bamboo charcoal particles, 20-25% of yeast fermentation material and 5-10% of composite microbial inoculum;
the lower layer comprises the following raw materials in percentage by weight: 20-30% of bamboo powder, 20-30% of humic acid, 10-20% of plant source amino acid, 10-15% of vegetable cake, 10-15% of earthworm excrement, 3-5% of medium trace elements and 5-10% of composite microbial inoculum;
the plant source amino acid is amino acid obtained by fermenting one or more of soybean, wheat, oat and corn;
the vegetable cake is a rapeseed cake;
the medium trace elements are a mixture of several of calcium, magnesium, zinc, manganese, boron, copper and iron;
the bamboo charcoal particles are solid substances generated by pyrolysis of bamboo at the high temperature of 500-600 ℃ under the condition of oxygen deficiency, and the particle size of the bamboo charcoal particles is 2-5 mm;
the composite microbial inoculum is one or more of bacillus subtilis, paenibacillus polymyxa, trichoderma viride and paecilomyces lilacinus.
2. The special culture medium for dendrobium nobile lindl as claimed in claim 1, wherein the thickness of the lower layer is twice that of the upper layer.
3. The special culture medium for dendrobium nobile according to claim 1, wherein the upper layer comprises the following raw materials by weight percent: 45% of pine bark, 25% of bamboo charcoal particles, 23% of yeast fermentation material and 7% of composite microbial inoculum.
4. The special culture medium for dendrobium nobile according to claim 1, wherein the lower layer comprises the following raw materials by weight percent: 25% of bamboo powder, 25% of humic acid, 10% of plant source amino acid, 10% of vegetable cake, 15% of earthworm excrement, 5% of medium trace elements and 10% of composite microbial inoculum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710258252.8A CN107047258B (en) | 2017-04-19 | 2017-04-19 | Special culture medium for dendrobium nobile lindl |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710258252.8A CN107047258B (en) | 2017-04-19 | 2017-04-19 | Special culture medium for dendrobium nobile lindl |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107047258A CN107047258A (en) | 2017-08-18 |
| CN107047258B true CN107047258B (en) | 2021-01-08 |
Family
ID=59600388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710258252.8A Active CN107047258B (en) | 2017-04-19 | 2017-04-19 | Special culture medium for dendrobium nobile lindl |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107047258B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439354A (en) * | 2017-09-20 | 2017-12-08 | 上海市药材有限公司 | Red sage root seedling medium and its feedstock composition, preparation method and application |
| CN107624608A (en) * | 2017-09-26 | 2018-01-26 | 上海辰山植物园 | A kind of cultivation matrix containing bamboo powder and preparation method thereof |
| CN108142242A (en) * | 2018-01-18 | 2018-06-12 | 兰溪百晟食品科技有限公司 | A kind of vegetables and fruits cultivation matrix |
| CN108174770A (en) * | 2018-01-18 | 2018-06-19 | 兰溪富晟食品科技有限公司 | Organic vegetable plantation substrate and preparation method thereof |
| CN108522213A (en) * | 2018-05-11 | 2018-09-14 | 江苏圣福来生态农业有限公司 | It is a kind of prevention dendrobium candidum black spot planting matrix and its application |
| CN108849388A (en) * | 2018-06-27 | 2018-11-23 | 天柱县佳源科技开发有限公司 | A kind of camellia mycorrhizal seedling raising matrix and its application method |
| CN108849389A (en) * | 2018-06-27 | 2018-11-23 | 天柱县佳源科技开发有限公司 | A kind of Resistant camellia seedling medium and its application method |
| CN108849387A (en) * | 2018-06-27 | 2018-11-23 | 天柱县佳源科技开发有限公司 | A kind of the camellia seedling medium and its application method of acid stable |
| CN108812183A (en) * | 2018-07-11 | 2018-11-16 | 合肥申沃园艺有限公司 | A kind of potting cultivation matrix and preparation method thereof |
| CN116508616A (en) * | 2023-03-16 | 2023-08-01 | 中国农业科学院油料作物研究所 | Mechanically transplanted rape strong seedling cultivation matrix and preparation method thereof |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103626610B (en) * | 2013-12-06 | 2016-03-30 | 时科生物科技（上海）有限公司 | A kind of Biologic organic bamboo charcoal fertilizer and production method thereof |
| CN104072305A (en) * | 2014-07-18 | 2014-10-01 | 时科生物科技（上海）有限公司 | Special bamboo charcoal bio-organic fertilizer for dendrobe and production method for bamboo charcoal bio-organic fertilizer |
| CN104140309B (en) * | 2014-08-03 | 2016-02-24 | 浙江瑞珏生物科技有限公司 | A kind of water conservation organic type stem of noble dendrobium special fertilizer |
| CN105724213A (en) * | 2016-02-27 | 2016-07-06 | 云南农业大学 | Formula of medium for planting dendrobium officinale |
| CN105766578B (en) * | 2016-03-11 | 2018-08-24 | 百色三宝生物技术有限公司 | Mushroom bran stem of noble dendrobium quality imitating wild planting process |
| CN105766591A (en) * | 2016-04-25 | 2016-07-20 | 广西智宝科技有限公司 | Dendrobium officinale culture medium and preparation method thereof |
| CN105850690A (en) * | 2016-06-27 | 2016-08-17 | 广西农业职业技术学院 | Dendrobium officinale culture medium |
| CN106171888B (en) * | 2016-07-18 | 2020-01-31 | 福建连天福生物科技有限公司 | Dendrobium officinale culture medium and culture method |
| CN106538371A (en) * | 2016-11-25 | 2017-03-29 | 巫溪县琼海农业有限公司 | The preparation method of dendrobium officinale culture medium |
-
2017
- 2017-04-19 CN CN201710258252.8A patent/CN107047258B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107047258A (en) | 2017-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107047258B (en) | Special culture medium for dendrobium nobile lindl | |
| CN109971680B (en) | Preparation and application of compound microbial agent with disease-resistant and growth-promoting functions | |
| CN104429581B (en) | A kind of Organic tomato health cultural method | |
| CN106171888B (en) | Dendrobium officinale culture medium and culture method | |
| CN111394281A (en) | Bacillus belgii BV03 microbial agent and preparation method and application thereof | |
| CN110156541A (en) | A kind of restorative procedure of secondary salinization soil | |
| CN108633669A (en) | The cultural method of ripe short cowpea variety summer open country of overgrowing in a kind of South China | |
| CN113455322A (en) | Continuous cropping-resistant efficient cultivation method for facility cowpea | |
| CN101283653B (en) | Fritillaria unibracteata plants drug and artificial cultivation method of bulb thereof | |
| CN110218678B (en) | Anti-continuous cropping soil remediation agent and preparation method thereof | |
| CN108738442B (en) | Tea-oil camellia forest soil curing system and manufacturing method thereof | |
| CN108651180B (en) | Ecological cultivation technology for promoting fast growth of newly-built bamboo forest | |
| CN103274837A (en) | Polyelement organic wintersweet compound fertilizer | |
| CN113039989A (en) | Intelligent ecological planting method for changing selenium-rich orchard into soil and controlling grass in hilly and mountainous areas | |
| CN105519398B (en) | Container seedling raising method for special valuable tree species corbicula fluminea at karst | |
| CN108901751B (en) | Solid matrix suitable for three-dimensional planting of vegetables and planting method | |
| CN108191504B (en) | Preparation method and use method of special fertilizer for konjak | |
| CN111133962A (en) | Cultivation method for increasing fruit setting amount of pod peppers | |
| CN112544383B (en) | Ecological cultivation method for intercropping pinellia ternate and soybeans | |
| CN111727847B (en) | Straw fiber seedling culture substrate for colonization biocontrol bacteria, production method thereof and two-step colonization biocontrol bacteria method | |
| CN114651698A (en) | Preparation method of leaf vegetable seedling raising substrate with cow dung fermentation product as main raw material, product and application thereof | |
| CN108967366B (en) | Method for preparing wormcast neutral fertilizer | |
| CN107652026B (en) | Special disease-resistant fertilizer for fingered citron and Chinese yam and preparation method thereof | |
| CN110024611B (en) | Method for improving disease resistance of betel nuts through arecoline treatment and application | |
| CN107182655A (en) | A kind of rice production technique |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A specialized cultivation substrate for Dendrobium officinale Effective date of registration: 20230628 Granted publication date: 20210108 Pledgee: Industrial Bank Co.,Ltd. Shanghai Minhang sub branch Pledgor: SEEK BIO-TECHNOLOGY (SHANGHAI) Co.,Ltd. Registration number: Y2023310000316 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20231013 Granted publication date: 20210108 Pledgee: Industrial Bank Co.,Ltd. Shanghai Minhang sub branch Pledgor: SEEK BIO-TECHNOLOGY (SHANGHAI) Co.,Ltd. Registration number: Y2023310000316 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A specialized cultivation substrate for Dendrobium officinale Effective date of registration: 20231129 Granted publication date: 20210108 Pledgee: Industrial Bank Co.,Ltd. Shanghai Minhang sub branch Pledgor: SEEK BIO-TECHNOLOGY (SHANGHAI) Co.,Ltd. Registration number: Y2023310000788 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |