CN106538371A - The preparation method of dendrobium officinale culture medium - Google Patents
The preparation method of dendrobium officinale culture medium Download PDFInfo
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- CN106538371A CN106538371A CN201611052729.9A CN201611052729A CN106538371A CN 106538371 A CN106538371 A CN 106538371A CN 201611052729 A CN201611052729 A CN 201611052729A CN 106538371 A CN106538371 A CN 106538371A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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Abstract
The invention belongs to planting technology field, more particularly to a kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：S1. Cortex Pini pretreatment；S2. coconut husk, sheep stool pretreatment；S3. rubble, wood shavings pretreatment；S4. prepared by bottom substrate；S5. prepared by surface layer substrate；S6. lay molding.Cultivation matrix candidum tissue culturing seedling transplanting survival rate obtained in herein described method is high, up to 86.67%；Sufficient nutritional labeling is provided for Herba Dendrobii, the growth of Herba Dendrobii plant and root system is effectively facilitated.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of preparation method of dendrobium officinale culture medium.
Background technology
Herba Dendrobii is orchid family Dendrobium herbaceous plant.Wild Herba Dendrobii to be grown nonparasitically upon another plant and have lichen up to 1600 meters in height above sea level
On the limestone of plant or tree, the ground such as Chinese Anhui, Zhejiang, Fujian are distributed mainly on, its stem is used as medicine.Herba Dendrobii sweet in the mouth, property
It is slightly cold, with reinforcing stomach reg fluid, nourishing YIN and clearing away heat, lung moistening kidney tonifying, the strong waist of item, antitumor, defying age, enhancing immunity, expansion blood
The effects such as pipe.
Dendrobium officinale growing environment is unique, very strict to climate requirements, grows nonparasitically upon another plant on trunk or sylvan life, generally with
The mixed life of lichens, moss and pteridophyta, unstable sufficient nutrition supply, no endosperm, poor growth, survival rate pole
It is low.With the development of tissue culture technique, artificial growth Herba Dendrobii is possibly realized, but its tissue cultured seedling transplanting survival rate is low.
The content of the invention
In view of this, it is an object of the invention to provide a kind of dendrobium officinale culture medium.
To realize object above, the technical scheme is that：
A kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：
S1. Cortex Pini pretreatment：Cortex Pini is sterilized after being placed in water immersion 4-7 days, is then comminuted into a diameter of 0.81-
The Cortex Pini granule of the Cortex Pini granule and a diameter of 0.5-0.8cm of 1.0cm；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection respectively, ferment, product powder after then ferment coconut husk
The coconut husk granule of a diameter of 0.75-0.96cm is broken into, product is ground into the sheep stool of a diameter of 0.35-0.45cm after sheep stool fermentation
Grain；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 0.81-1.0cm, through the rubble and a diameter of of sterilization
The coconut husk granule of a diameter of 0.75-0.96cm is according to 3-4:2-3:The quality of 2-3 compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.5-0.8cm, through the wood shavings and a diameter of of sterilization
The sheep stool granule of 0.35-0.45cm is according to 4-5:1-2:The quality of 2-3 compares mix homogeneously；
S6. lay molding：Surface layer substrate is laid in bottom substrate, the dendrobium officinale culture medium is obtained final product.
Further, 2-3 times for Cortex Pini quality of the consumption of the water.
Further, the thickness of surface layer substrate is 1-2cm, and the thickness of bottom substrate is 5-10cm.
Further, it is 50% that summer keeps soil humidity；It is 20-30% that winter keeps soil humidity；Spring, autumn keep
Soil humidity is 35-45%.
Further, the sterilization is referred in the solution after 800-1200 times of 50% thiophanate methyl wettable powder dilution agent
Immersion 2-3h.
Further, the fermentation is aerobic fermentation.
Further, temperature is controlled in sweat for 35-38 DEG C, relative air humidity is 52-55%, pressure inside the tank of fermenting
For 0.021-0.025MPa, air capacity is passed through for 750-770m3/ h, fermentation time are 5-8 days.
The beneficial effects of the present invention is：
Cultivation matrix candidum tissue culturing seedling transplanting survival rate obtained in herein described method is high, up to 86.67%；For
Herba Dendrobii provides sufficient nutritional labeling, effectively facilitates the growth of Herba Dendrobii plant and root system.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.It is unreceipted in preferred embodiment
The experimental technique of actual conditions, generally according to normal condition.
Embodiment 1
A kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：
S1. Cortex Pini pretreatment：Cortex Pini be placed in water 2 times of Cortex Pini quality (consumption for) sterilization after immersion 4 days (in
2-3h is soaked in solution after 50% 800 times of thiophanate methyl wettable powder dilution agent), it is then comminuted into a diameter of 1.0cm's
The Cortex Pini granule of Cortex Pini granule and a diameter of 0.5cm；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection (dilute in 50% thiophanate methyl wettable powder respectively
Soak 2h in releasing the solution after 800 times), aerobic fermentation (control temperature for 35 DEG C, relative air humidity is 55%, in fermentation tank
Pressure is 0.025MPa, is passed through air capacity for 750m3/ h, fermentation time are 5 days), after then ferment coconut husk, product is ground into
The coconut husk granule of a diameter of 0.75cm, after sheep stool fermentation, product is ground into the sheep stool granule of a diameter of 0.35cm；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection (in 50% thiophanate methyl wettable powder dilution agent
2h is soaked in solution after 800 times)；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 1.0cm, through the rubble and a diameter of diameter of sterilization
For 0.75cm coconut husk granule according to 4:2:2 quality compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.5cm, through the wood shavings and a diameter of of sterilization
The sheep stool granule of 0.35cm is according to 5:1:2 quality compares mix homogeneously；
S6. lay molding：It is that 1cm surface layer substrate is laid in the bottom substrate that thickness is 5cm by thickness, summer keeps base
Matter humidity is 50%；It is 20% that winter keeps soil humidity；It is 35% that spring, autumn keep soil humidity；Obtain final product the iron sheet
Dendrobe cultivation substrate.
Embodiment 2
A kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：
S1. Cortex Pini pretreatment：Cortex Pini be placed in water 3 times of Cortex Pini quality (consumption for) sterilization after immersion 7 days (in
3h is soaked in solution after 50% 1200 times of thiophanate methyl wettable powder dilution agent), it is then comminuted into a diameter of 0.81cm's
The Cortex Pini granule of Cortex Pini granule and a diameter of 0.8cm；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection (dilute in 50% thiophanate methyl wettable powder respectively
Soak 3h in releasing the solution after 1200 times), aerobic fermentation (control temperature for 38 DEG C, relative air humidity is 52%, in fermentation tank
Pressure is 0.021MPa, is passed through air capacity for 770m3/ h, fermentation time are 8 days), after then ferment coconut husk, product is ground into
The coconut husk granule of a diameter of 0.96cm, after sheep stool fermentation, product is ground into the sheep stool granule of a diameter of 0.45cm；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection (in 50% thiophanate methyl wettable powder dilution agent
3h is soaked in solution after 1200 times)；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 0.81cm, through the rubble and a diameter of diameter of sterilization
For 0.96cm coconut husk granule according to 3:3:3 quality compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.8cm, through the wood shavings and a diameter of of sterilization
The sheep stool granule of 0.45cm is according to 4:2:3 quality compares mix homogeneously；
S6. lay molding：It is that 2cm surface layer substrate is laid in the bottom substrate that thickness is 10cm by thickness, summer keeps
Soil humidity is 50%；It is 30% that winter keeps soil humidity；It is 45% that spring, autumn keep soil humidity；Obtain final product the ferrum
Skin dendrobe cultivation substrate.
Embodiment 3
A kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：
S1. Cortex Pini pretreatment：Cortex Pini is placed in immersion in water (2.5 times for Cortex Pini quality of consumption) and sterilizes after 5 days
(soaking 2.5h in the solution after 1000 times of 50% thiophanate methyl wettable powder dilution agent), is then comminuted into a diameter of
The Cortex Pini granule of the Cortex Pini granule and a diameter of 0.63cm of 0.95cm；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection (dilute in 50% thiophanate methyl wettable powder respectively
Soak 2.5h in releasing the solution after 1000 times), aerobic fermentation (control temperature for 36 DEG C, relative air humidity is 54%, fermentation tank
Interior pressure is 0.023MPa, is passed through air capacity for 760m3/ h, fermentation time are 6 days), after then ferment coconut husk, product is crushed
Into the coconut husk granule of a diameter of 0.84cm, after sheep stool fermentation, product is ground into the sheep stool granule of a diameter of 0.42cm；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection (in 50% thiophanate methyl wettable powder dilution agent
2.5h is soaked in solution after 1000 times)；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 0.95cm, through the rubble and a diameter of diameter of sterilization
For 0.84cm coconut husk granule according to 4:3:3 quality compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.6cm, through the wood shavings and a diameter of of sterilization
The sheep stool granule of 0.42cm is according to 5:2:3 quality compares mix homogeneously；
S6. lay molding：It is that 1.5cm surface layer substrate is laid in the bottom substrate that thickness is 8cm by thickness, summer keeps
Soil humidity is 50%；It is 25% that winter keeps soil humidity；It is 40% that spring, autumn keep soil humidity；Obtain final product the ferrum
Skin dendrobe cultivation substrate.
Comparative example 1
A kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：
S1. Cortex Pini pretreatment：Cortex Pini sterilization is (molten after 900 times of 50% thiophanate methyl wettable powder dilution agent
3h is soaked in liquid), it is then comminuted into the Cortex Pini granule of the Cortex Pini granule and a diameter of 0.7cm of a diameter of 0.86cm；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection (dilute in 50% thiophanate methyl wettable powder respectively
Soak 3h in releasing the solution after 900 times), aerobic fermentation (control temperature for 37 DEG C, relative air humidity is 54%, in fermentation tank
Pressure is 0.022MPa, is passed through air capacity for 750m3/ h, fermentation time are 6 days), after then ferment coconut husk, product is ground into
The coconut husk granule of a diameter of 0.81cm, after sheep stool fermentation, product is ground into the sheep stool granule of a diameter of 0.36cm；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection (in 50% thiophanate methyl wettable powder dilution agent
3h is soaked in solution after 900 times)；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 0.86cm, through the rubble and a diameter of diameter of sterilization
For 0.81cm coconut husk granule according to 3:2:2 quality compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.7cm, through the wood shavings and a diameter of of sterilization
The sheep stool granule of 0.36cm is according to 5:1:3 quality compares mix homogeneously；
S6. lay molding：It is that 1cm surface layer substrate is laid in the bottom substrate that thickness is 7cm by thickness, summer keeps base
Matter humidity is 50%；It is 24% that winter keeps soil humidity；It is 42% that spring, autumn keep soil humidity；Obtain final product the iron sheet
Dendrobe cultivation substrate.
Comparative example 2
A kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：
S1. Cortex Pini pretreatment：Cortex Pini be placed in water 3 times of Cortex Pini quality (consumption for) sterilization after immersion 5 days (in
2h is soaked in solution after 50% 1100 times of thiophanate methyl wettable powder dilution agent), it is then comminuted into a diameter of 0.87cm's
The Cortex Pini granule of Cortex Pini granule and a diameter of 0.5cm；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection (dilute in 50% thiophanate methyl wettable powder respectively
Soak 3h in releasing the solution after 1100 times), aerobic fermentation (control temperature for 37 DEG C, relative air humidity is 55%, in fermentation tank
Pressure is 0.024MPa, is passed through air capacity for 770m3/ h, fermentation time are 7 days), after then ferment coconut husk, product is ground into
The coconut husk granule of a diameter of 0.78cm, after sheep stool fermentation, product is ground into the sheep stool granule of a diameter of 0.4cm；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection (in 50% thiophanate methyl wettable powder dilution agent
3h is soaked in solution after 1100 times)；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 0.87cm, through the rubble and a diameter of diameter of sterilization
For 0.78cm coconut husk granule according to 5:1:3 quality compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.5cm, through the wood shavings and a diameter of 0.4cm of sterilization
Sheep stool granule according to 4:2:3 quality compares mix homogeneously；
S6. lay molding：It is that 2cm surface layer substrate is laid in the bottom substrate that thickness is 10cm by thickness, summer keeps
Soil humidity is 50%；It is 28% that winter keeps soil humidity；It is 39% that spring, autumn keep soil humidity；Obtain final product the ferrum
Skin dendrobe cultivation substrate.
Comparative example 3
A kind of preparation method of dendrobium officinale culture medium, which concretely comprises the following steps：
S1. Cortex Pini pretreatment：Cortex Pini is placed in immersion in water (2.5 times for Cortex Pini quality of consumption) and sterilizes after 6 days
(soaking 2h in the solution after 1200 times of 50% thiophanate methyl wettable powder dilution agent), is then comminuted into a diameter of 1.0cm
Cortex Pini granule and a diameter of 0.6cm Cortex Pini granule；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection (dilute in 50% thiophanate methyl wettable powder respectively
Soak 2h in releasing the solution after 1200 times), aerobic fermentation (control temperature for 38 DEG C, relative air humidity is 52%, in fermentation tank
Pressure is 0.022MPa, is passed through air capacity for 757m3/ h, fermentation time are 7 days), after then ferment coconut husk, product is ground into
The coconut husk granule of a diameter of 0.75cm, after sheep stool fermentation, product is ground into the sheep stool granule of a diameter of 0.35cm；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection (in 50% thiophanate methyl wettable powder dilution agent
2h is soaked in solution after 1200 times)；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 1.0cm, through the rubble and a diameter of diameter of sterilization
For 0.75cm coconut husk granule according to 3:2:2 quality compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.6cm, through the wood shavings and a diameter of of sterilization
The sheep stool granule of 0.35cm is according to 5:3:4 quality compares mix homogeneously；
S6. lay molding：It is that 1.5cm surface layer substrate is laid in the bottom substrate that thickness is 7.5cm by thickness, summer protects
Soil humidity is held for 50%；It is 27% that winter keeps soil humidity；It is 38% that spring, autumn keep soil humidity；Obtain final product described
Dendrobium officinale culture medium.
By 5-7 days after dendrobium candidum axenic tissue cultured seedling (high 3-4cm, root, stem, leaf are complete) seedling exercising, 6 are then randomly divided into
Group, transplants to embodiment 1-3 and comparative example 1-3 according to line-spacing 13cm, spacing in the rows 9cm respectively and is trained on the cultivation matrix for preparing
Support, during culture, way to manage is identical.After transplanting 1 year, statistics survival rate, plant height, stalk rugosity, root length, radical, knot
It is really as shown in the table：
Finally illustrate, above example is only unrestricted to illustrate technical scheme, although with reference to compared with
Good embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent, and without deviating from the objective and scope of technical solution of the present invention, which all should be covered at this
In the middle of the right of invention.
Claims (7)
1. a kind of preparation method of dendrobium officinale culture medium, it is characterised in that concretely comprise the following steps：
S1. Cortex Pini pretreatment：Cortex Pini is sterilized after being placed in water immersion 4-7 days, is then comminuted into a diameter of 0.81-1.0cm
Cortex Pini granule and a diameter of 0.5-0.8cm Cortex Pini granule；
S2. coconut husk, sheep stool pretreatment：Coconut husk, sheep stool carry out disinfection respectively, ferment, and after then ferment coconut husk, product is ground into
The coconut husk granule of a diameter of 0.75-0.96cm, after sheep stool fermentation, product is ground into the sheep stool granule of a diameter of 0.35-0.45cm；
S3. rubble, wood shavings pretreatment：Rubble, wood shavings are carried out disinfection；
S4. prepared by bottom substrate：By the Cortex Pini granule of a diameter of 0.81-1.0cm, through the rubble and a diameter of diameter of sterilization
For 0.75-0.96cm coconut husk granule according to 3-4:2-3:The quality of 2-3 compares mix homogeneously；
S5. prepared by surface layer substrate：By the Cortex Pini granule of a diameter of 0.5-0.8cm, through the wood shavings and a diameter of 0.35- of sterilization
The sheep stool granule of 0.45cm is according to 4-5:1-2:The quality of 2-3 compares mix homogeneously；
S6. lay molding：Surface layer substrate is laid in bottom substrate, the dendrobium officinale culture medium is obtained final product.
2. a kind of preparation method of dendrobium officinale culture medium according to claim 1, it is characterised in that the use of the water
Measure 2-3 times for Cortex Pini quality.
3. the preparation method of a kind of dendrobium officinale culture medium according to claim 1, it is characterised in that surface layer substrate
Thickness is 1-2cm, and the thickness of bottom substrate is 5-10cm.
4. the preparation method of a kind of dendrobium officinale culture medium according to claim 1, it is characterised in that summer keeps base
Matter humidity is 50%；It is 20-30% that winter keeps soil humidity；It is 35-45% that spring, autumn keep soil humidity.
5. the preparation method of a kind of dendrobium officinale culture medium according to claim 1, it is characterised in that the sterilization is
Refer to 2-3h is soaked in the solution after 800-1200 times of 50% thiophanate methyl wettable powder dilution agent.
6. the preparation method of a kind of dendrobium officinale culture medium according to claim 1, it is characterised in that the fermentation is
Aerobic fermentation.
7. the preparation method of a kind of dendrobium officinale culture medium according to claim 6, it is characterised in that in sweat
Temperature is controlled for 35-38 DEG C, relative air humidity is 52-55%, fermentation pressure inside the tank is 0.021-0.025MPa, is passed through air
Measure as 750-770m3/ h, fermentation time are 5-8 days.
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| CN106966809A (en) * | 2017-04-21 | 2017-07-21 | 山东沃森农业科技有限公司 | A kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling |
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| CN109691375A (en) * | 2019-02-11 | 2019-04-30 | 安徽农业大学 | A kind of dendrobium officinale culture medium and its preparation method and application based on pecan shell |
| CN110169315A (en) * | 2019-06-06 | 2019-08-27 | 贺州市天林果蔬种植专业合作社 | A kind of implantation methods of chaetomers melon |
| CN112753532A (en) * | 2021-01-11 | 2021-05-07 | 彭贵阳 | Substrate formula and seedbed for dendrobium during seedling hardening period |
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| CN109691375A (en) * | 2019-02-11 | 2019-04-30 | 安徽农业大学 | A kind of dendrobium officinale culture medium and its preparation method and application based on pecan shell |
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