CN106966809A - A kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling - Google Patents

A kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling Download PDF

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Publication number
CN106966809A
CN106966809A CN201710264247.8A CN201710264247A CN106966809A CN 106966809 A CN106966809 A CN 106966809A CN 201710264247 A CN201710264247 A CN 201710264247A CN 106966809 A CN106966809 A CN 106966809A
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CN
China
Prior art keywords
parts
tissue culturing
tamed
culturing seedling
candidum tissue
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Pending
Application number
CN201710264247.8A
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Chinese (zh)
Inventor
刘中华
迟斐斐
林立红
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Shandong Watson Agriculture Technology Co Ltd
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Shandong Watson Agriculture Technology Co Ltd
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Priority to CN201710264247.8A priority Critical patent/CN106966809A/en
Publication of CN106966809A publication Critical patent/CN106966809A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling, formed by the raw material compounding fermentation comprising following component:10 20 parts of sheep manure, 5~10 parts of rabbit excrement, 6~8 parts of rape cake, 4~6 parts of silkworm excrement, 10~20 parts of Tea Saponin, 1~2 part of matrine, 0.5~1 part of quick lime, 80~100 parts of pine bark.The invention also discloses the preparation method of the above-mentioned insect prevention cultivation matrix tamed for candidum tissue culturing seedling.The compounding cultivation matrix of the inventive method production contains the organic matters such as sheep manure, rabbit excrement, silkworm excrement, can meet needed for candidum tissue culturing seedling domesticating and cultivating, the administration of base manure need not be carried out within latter year by cultivating;Quick lime, Tea Saponin and matrine are with the addition of inside component in the present invention, the generation of the insect pests such as snail, slug, cabbage caterpillar can be effectively suppressed in cultivation, and is avoided that above-mentioned insect lays eggs and large-scale outburst in matrix.

Description

A kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling
Technical field
The present invention relates to arviculture field, and in particular to a kind of insect prevention cultivation tamed for candidum tissue culturing seedling Matrix.
Background technology
Dendrobium candidum is orchid family Dendrobium Sw, is a kind of rare Chinese medicine, with nourishing the stomach of promoting the production of body fluid, nourishing Yin and clearing heat, profit The effects such as lung kidney-nourishing.In recent years, going deep into dendrobium candidum efficacy study, the market demand is increasing, cultivating and growing demand Also constantly expand;Its kind can be improved by being concentrated by candidum tissue culturing seedling after domestication, then being transplanted to progress field planting in greenhouse The survival rate of seedling, the utilization rate for shortening warmhouse booth facility in growth cycle, raising unit area;But in tissue-cultured seedling domesticating and cultivating During, due to the generation for the insect pest large area that the factors such as environment, weather are caused, it drastically influence dendrobium candidum intensive tame and docile Change, therefore, the research that the stage of being tamed to candidum tissue culturing seedling prevents that insect pest large area from occurring is extremely urgent;Further, since iron The intensive domestication of the skin stem of noble dendrobium almost can not find space than comparatively dense, the administration of organic fertilizer, and the current intensive domestication of dendrobium candidum is also Mainly based on the spraying of foliar fertilizer, which results in domestication seedling quality is not high, the problems such as disease resistance is poor.
The content of the invention
The technical problem to be solved in the present invention is that the large area for preventing candidum tissue culturing seedling from taming stage insect pest occurs.
In order to solve the above technical problems, the present invention is adopted the technical scheme that:
A kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling, is compounded by the raw material comprising following component and fermented Form:10-20 parts of sheep manure, 5~10 parts of rabbit excrement, 6~8 parts of rape cake, 4~6 parts of silkworm excrement, 10~20 parts of Tea Saponin, matrine 1~ 2 parts, 0.5~1 part of quick lime, 80~100 parts of pine bark.
Sheep manure, rabbit excrement, silkworm excrement, rape cake are the good organic fertilizer of a class afterwards by fermentation, and Nitrogen-and Phosphorus-containing potassium is balanced, uses it Fertilizer is made, available nutrient and the content of organic matter in matrix can be strengthened, and is difficult burn seedlings, it can be iron to be mixed with pine bark after fermentation The skin stem of noble dendrobium provides organic nutrition;Contain triterpene saponin in Tea Saponin, can effectively kill the insects such as cutworm, nematode, snail, and And soil pollution is not resulted in, be conducive to environmental protection;Matrine is a kind of low toxicity, low-residual, eco-friendly pesticide, main preventing and treating The insects such as various pine moths, Euproctis pseudoconspersa, cabbage caterpillar, with a variety of work(such as insecticidal activity, bactericidal activity, coordinate plant growth functions Energy:Pine bark is dendrobium officinale culture medium, and quick lime progress fermentation is added in these raw materials and is conducive to cultivating in a short time Matrix can ferment fully, make respectively to be used for the cultivation of seedlings of Dendrobium officinale.
It is preferred that, the insect prevention cultivation matrix is formed by the raw material compounding fermentation comprising following component:15-20 parts of sheep manure, 8~10 parts of rabbit, 6~8 parts of rape cake, 5~6 parts of silkworm excrement, 15~20 parts of Tea Saponin, 1~2 part of matrine, 0.5~1 part of quick lime, 90~100 parts of pine bark.
Above-mentioned is used for the preparation method for the insect prevention cultivation matrix that candidum tissue culturing seedling is tamed, and comprises the following steps:
(1) by the sheep manure of formula ratio, rabbit excrement, rape cake, silkworm excrement, Tea Saponin part, matrine and quick lime, crush, mixing, It is added to after stirring in 80~100 parts of pine barks, it is well mixed to obtain base starting material;
(2) after the base starting material obtained in step (1) is added water, film sealing, pile fermentation is produced.
In step (1), the sheep manure is 10-20 parts, rabbit excrement is 5~10 parts, rape cake is 6~8 parts, silkworm excrement is 4~6 Part, Tea Saponin be 10~20 parts, matrine be 1~2 part, quick lime be 0.5~1 part.
In step (1), the particle diameter of the pine bark is 1~1.5cm.
In step (2), the mass ratio of the base starting material and water is 1:0.5~1:2.
In step (2), the time of the fermentation is 40~60 days.
In step (2), the temperature of the fermentation is 10~25 DEG C of environment temperature.
Beneficial effect:
The compounding cultivation matrix of the inventive method production contains the organic matters such as sheep manure, rabbit excrement, silkworm excrement, can meet iron sheet stone Needed for dry measure used in former times tissue-cultured seedling domesticating and cultivating, the administration of base manure need not be carried out within latter year by cultivating;
Quick lime, Tea Saponin and matrine are with the addition of inside component in the present invention, can effectively suppress snail, Kuo in cultivation The generation of the insect pests such as Yu, cabbage caterpillar, and it is avoided that above-mentioned insect lays eggs and large-scale outburst in matrix.
Embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real Apply the content described by example and be merely to illustrate the present invention, without should be also without limitation on sheet described in detail in claims Invention.
Embodiment 1
It is a kind of can pest control occur candidum tissue culturing seedling domestication organic culture substrate, its preparation method include it is following Step:
(1) by 15 parts of sheep manure, 8 parts of rabbit excrement, 6 parts of rape cake, 6 parts of silkworm excrement, 20 parts of Tea Saponin, 2 parts of matrine, quick lime 0.5 part, after all being crushed by proportioning, it is mixed evenly;
(2) raw material after stirring is added in 100 parts of pine barks, and well mixed forms base complex;
(3) handled after above-mentioned compound is added water with plastic film seal pile fermentation, the mass ratio of compound and water is 1: 0.5;
(4) after pile fermentation 60d, you can carry out the domestication organic culture substrate of candidum tissue culturing seedling.
(5) above-mentioned pine bark specification is 1cm or so particle;
Embodiment 2
It is a kind of can pest control occur candidum tissue culturing seedling domestication organic culture substrate, its preparation method include it is following Step:
(1) by 20 parts of sheep manure, 10 parts of rabbit excrement, 8 parts of rape cake, 5 parts of silkworm excrement, 15 parts of Tea Saponin, 1 part of matrine, quick lime 1 Part, after all being crushed by proportioning, it is mixed evenly;
(2) raw material after stirring is added in 90 parts of pine barks, and well mixed forms base complex;
(3) handled after above-mentioned compound is added water with plastic film seal pile fermentation, the mass ratio of compound and water is 1: 0.5;
(4) after pile fermentation 40d, you can carry out the domestication organic culture substrate of candidum tissue culturing seedling.
(5) above-mentioned pine bark specification is 1.5cm or so particle.
Embodiment 3
The influence that different cultivation medium formulas grow to snail
1. test material
Cultivation matrix, Bradybaena similaris Ferussac, candidum tissue culturing seedling described in embodiment 1 and embodiment 2.
2. test method
Cultivation matrix described in embodiment 1 and embodiment 2 is carried out after high-temperature sterilization, is laid in liftoff shade greenhouse On seedbed.Shade greenhouse uses shade rate for 75% rain-proof greenhouse, greenhouse long 35m, wide 8m, canopy built-in wide 1.4m, long 32m, Seedbed high 0.8m from the ground, and make with screen cloth the substrate of matrix.The thickness of matrix laying is 10cm.The cultivation matrix of control group is Pure pine bark.
Bradybaena similaris Ferussac is mixed into above-mentioned cultivation matrix, inoculum density is 50/100L, sets 5 repetitions, and in base Candidum tissue culturing seedling is planted in matter, planting density is 15cm*15cm, 4 plants/clump.Daily cultivation management is carried out after domestication plantation.
Every 30 days statistics once remaining worm amount, and count Revision insect recluced rate=[(worm living after before processing work borer population-processing Number)/before processing work borer population] × 100%;
3. result is shown in Table 1 as follows, it is found by experiment that, the substrate combination of embodiment 1 and embodiment 2 has to snail harm Good prevention effect.Control group without carrying out any processing, after snail is inoculated with 3 months, its insect population number does not subtract not only It is few significantly to increase on the contrary, illustrate that snail has carried out amount reproduction in control group matrix.
The influence that the different cultivation matrixes of table 1 are endangered snail

Claims (6)

1. a kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling, it is characterised in that by the original for including following component Material compounding fermentation is formed:10-20 parts of sheep manure, 5~10 parts of rabbit excrement, 6~8 parts of rape cake, 4~6 parts of silkworm excrement, Tea Saponin 10~20 Part, 1~2 part of matrine, 0.5~1 part of quick lime, 80~100 parts of pine bark.
2. being used for the preparation method for the insect prevention cultivation matrix that candidum tissue culturing seedling is tamed described in claim 1, its feature exists In comprising the following steps:
(1) by the sheep manure of formula ratio, rabbit excrement, rape cake, silkworm excrement, Tea Saponin part, matrine and quick lime, crush, mix, stirring It is added to after uniform in pine bark, it is well mixed to obtain base starting material;
(2) after the base starting material obtained in step (1) is added water, film sealing, pile fermentation is produced.
3. preparation method according to claim 2, it is characterised in that in step (1), the pine bark particle diameter is 1~ 1.5cm。
4. preparation method according to claim 2, it is characterised in that in step (2), the quality of the base starting material and water Than for 1:0.5~1:2.
5. preparation method according to claim 2, it is characterised in that in step (2), the time of the fermentation is 40~60 My god.
6. preparation method according to claim 2, it is characterised in that in step (2), the temperature of the fermentation is environment temperature 10~25 DEG C of degree.
CN201710264247.8A 2017-04-21 2017-04-21 A kind of insect prevention cultivation matrix tamed for candidum tissue culturing seedling Pending CN106966809A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1296753A (en) * 2000-09-30 2001-05-30 苗振东 Plant insecticide and its preparing process
CN102792951A (en) * 2012-03-21 2012-11-28 张文龙 Insecticidal fungicide with matrine and tea saponin
CN105850690A (en) * 2016-06-27 2016-08-17 广西农业职业技术学院 Dendrobium officinale culture medium
CN105884476A (en) * 2014-09-09 2016-08-24 许超群 Culture medium of dendrobium candidum
CN106538371A (en) * 2016-11-25 2017-03-29 巫溪县琼海农业有限公司 The preparation method of dendrobium officinale culture medium

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1296753A (en) * 2000-09-30 2001-05-30 苗振东 Plant insecticide and its preparing process
CN102792951A (en) * 2012-03-21 2012-11-28 张文龙 Insecticidal fungicide with matrine and tea saponin
CN105884476A (en) * 2014-09-09 2016-08-24 许超群 Culture medium of dendrobium candidum
CN105850690A (en) * 2016-06-27 2016-08-17 广西农业职业技术学院 Dendrobium officinale culture medium
CN106538371A (en) * 2016-11-25 2017-03-29 巫溪县琼海农业有限公司 The preparation method of dendrobium officinale culture medium

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Application publication date: 20170721

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