CN103004437B - Acacia mearnsii adult seed tree rejuvenation cottage seedling raising method - Google Patents
Acacia mearnsii adult seed tree rejuvenation cottage seedling raising method Download PDFInfo
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- CN103004437B CN103004437B CN201210564948.0A CN201210564948A CN103004437B CN 103004437 B CN103004437 B CN 103004437B CN 201210564948 A CN201210564948 A CN 201210564948A CN 103004437 B CN103004437 B CN 103004437B
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Abstract
The invention discloses an acacia mearnsii adult seed tree rejuvenation cottage seedling raising method, which comprises steps such as rejuvenation of adult seed trees, collection of scion wood, cutting wood treatment, treatment of seedling bed and cottage substrate, cottage, post management of cottage and seedling breeding. The method is characterized in that after rejuvenation treatment is carried out for the 5-year to 6-year strong acacia mearnsii adult seed tree which is selected in winter, scion wood is collected in spring and summer in the next year to be cut into the seedling bed with the cottage substrate, the acacia mearnsii cottage seedling is obtained after the post cottage management, after the cottage seedling is cultivated for one year, the cottage young seedling is collected again in spring and summer to be cut again, and finally the acacia mearsii seed tree is obtained. The scion wood which is obtained through the breeding method is excellent and pure in quality and easy for rooting, the method is simple to operate, the breeding coefficient is high, the excellent characteristics of the acacia mearnsii are maintained, the tannin content and the proanthocyanidin content of the cottage wood are high, and good economic benefit and social benefit can be realized.
Description
Technical field
The invention belongs to plant asexual multiplication technology field, relate to the method for the multiple young cuttage and seedling culture of the adult elite stand of a kind of black chaste tree.
Background technology
Black chaste tree (Acacia mearnsii De Willd.) has another name called Cortex Acacia decurrens, main product in South Africa, East Africa and Brazil etc. state.India, Indonesia, Philippine, Japan, Morocco, Sri Lanka and the U.S. etc. successively introduce a fine variety afforestation.China introduces a fine variety black chaste tree since the beginning of the fifties, there is distribution in Zhejiang, Fujian, Jiangsu, Guangxi, Jiangxi, Beijing, Guizhou, Hunan, Taiwan, Guangdong, Yunnan, Sichuan at present.
Black chaste tree is world-renowned fast-growing, high yield, high-quality tannin extract seeds, simultaneously or the raw tree species of proanthocyanidin, has the laudatory title of " forestry dark fund ".Acacia mearnsii proanthocyanidin all has obvious inhibitory action to leukaemia, hepatoma carcinoma cell, stomach cancer cell and human breast cancer cell etc., and Black Wattle Tannin Extract market development also enjoys common people to pay close attention to.But black chaste tree family is numerous, between different familys, tannin content and proanthocyanidin content difference are fairly obvious, true seed seedlings by sexual propagation is unstable, the more difficult merit maximum that allows is inherited, and can allow family merit be kept to greatest extent by cottage propagation, therefore, resolve black chaste tree cottage propagation complex art parameter, set up the good acacia mearnsii kind fast breeding technique system that tannin and proanthocyanidin content are high, for the whole nation provides high-quality, good acacia mearnsii seedling, tool is of great significance.
Summary of the invention
The object of the invention is to overcome the deficiency of existing black chaste tree seed sexual propagation mode, sets up tannin and proanthocyanidin content height and the metastable good acacia mearnsii kind fast breeding technique system of proterties, a kind of method that provides black chaste tree to grow up the multiple young cuttage and seedling culture of elite stand.
The present invention is achieved in that
The multiple young cuttage and seedling culture method of the adult elite stand of a kind of black chaste tree, comprise and managing after the processing, cuttage, cuttage of multiple children processings, the collection of fringe bar, cuttings processing, seedbed and the cutting medium of the elite stand that grows up and with the operation of Miao Fanmiao, after multiple children's processing by the adult elite stand of black chaste tree, gather tloig-cutlage in the seedbed of cutting medium is housed, by the management after cuttage, obtain black chaste tree cuttage seedling, the cultivation of gained cuttage seeding is carried out to cottage propagation after 1 year again and grow seedlings, main operational steps is as follows:
(1) the multiple children of adult elite stand processes: winter, select healthy and strong black chaste tree generation in 5 ~ 6 years year liftoff 60cm of an elite stand place cutting strunk, coat red paint or Wound-healing agent in wound;
(2) fringe bar gathers: after the coming year, two seasons of spring and summer gathered cutting strunk, the black chaste tree rudiment bar of trunk place germinating is as fringe bar;
(3) cuttings is processed: the long 10 ~ 12cm of clip fringe bar, remove unnecessary blade, and retain 3 ~ 5 wilson passionflower herbs of pinnate leaf, truncation fringe bar upper end, the fringe bar lower end of cutting sth. askew, 45 degree angles, then processes fringe bar;
(4) processing of seedbed and cutting medium: as cutting medium, cuttage is sterilized with 0.3 ~ 0.5% potassium permanganate solution the previous day, then covers with film with fresh yellow soil;
(5) the little shed of 80cm is built in cuttage: will process tloig-cutlage in matrix 4 ~ June, the cuttage degree of depth is 2 ~ 3cm, waters and drenches after having inserted above seedbed, covers film and shade net;
(6) after cuttage, manage: water or the carbendazim aqueous solution are obtained to cuttage seeding by atomizers spray cuttings;
(7) with Miao Fanmiao: cuttage seeding is cultivated, in two season of Second Year spring and summer clip branch carry out secondary cottage propagation.
The multiple young cuttage and seedling culture method of the adult elite stand of above-described a kind of black chaste tree, described processing fringe bar is with the CDP of 800 ~ 1000ppm or the ABT root-inducing powder aqueous solution and talcum powder furnishing root-growing agent, after the cuttings shearing is put into root-growing agent clockwise or stirred counterclockwise 30s, take out, cuttage is in matrix rapidly.
The multiple young cuttage and seedling culture method of the female elite stand that grows up of above-described a kind of black chaste tree, after described cuttage, management is after tloig-cutlage, uses atomizer spray 1 time, ventilation 30min every morning; By the time after growing callus, every other day spray 1 time, ventilation 60min, sprays 1 time with the clear carbendazim aqueous solution for every 5 days.
The present invention has the following advantages with respect to prior art:
1, the multiple young cuttage and seedling culture method of the adult elite stand of black chaste tree of the present invention, is the once breakthrough of the adult elite stand cuttage raise seedling technique of black chaste tree, has expanded the vegetative material source kind of black chaste tree.
2, the multiple young cuttage and seedling culture method of the adult elite stand of black chaste tree of the present invention, not only overcome the deficiency of black chaste tree seed sexual propagation mode, with respect to also having certain advantage with the black chaste tree elite stand of (life in 1 ~ 2 year) true seed seedling childhood as the cuttage and seedling culture of asexual propagation material: because the fringe bar gathering the is black chaste tree multiple sprout bar of elite stand of growing up, at this moment Black Wattle tannin and the performance of proanthocyanidin content proterties difference are obviously, be conducive to select elite stand that tannin and proanthocyanidin content are high as asexual propagation material, to passing on to greatest extent maternal merit, provide the more black chaste tree seedling of high-quality that powerful guarantee is provided.
3, the black chaste tree of the present invention multiple young cuttage and seedling culture method of elite stand of growing up, the raw material such as selected root-growing agent, cutting medium are easy to get, and prepare simple and conveniently, and propagation method is universal execution easily.
4, the multiple young cuttage and seedling culture method of the adult elite stand of black chaste tree of the present invention, can utilize growing seedlings of gained to carry out secondary cuttage with the method for Miao Fanmiao, can make full use of the resource of growing seedlings, and has shortened the cultivation time, produces ahead of time economic benefit.
5, the multiple young cuttage and seedling culture method of the adult elite stand of black chaste tree of the present invention, cost is low, simple to operate, and breeding is fast, and the nursery stock quantity of acquisition is large, possesses good genetic character and stabilization characteristics of genetics.
Embodiment
By specific embodiment given below, can further be well understood to the present invention, but they not limitation of the invention.
embodiment 1
Experimental field be positioned at scientific research nursery, forest-science academy, Guangxi.Nursery is positioned at 22 ° of 56 ' N, 108 ° of 21 ' E, height above sea level 80～100m, soil is that shale is grown the laterite forming, and belongs to moistening south subtropics monsoon climate, 20～21 ℃ of average annual temperature, 39.4 ℃ of the extreme highest temperatures, extreme lowest temperature is 1.5 ℃ of ﹣, 1 11.8 ℃ of monthly temperature, 7 27.6 ℃ of monthly temperature, average annual rainfall 1347.2mm.5 Nian Heijing of artificial culture in the garden multiple children of elite stand that grows up is processed, first November to the ripe liftoff 60cm of elite stand of black chaste tree place cutting strunk, in wound, coat red paint.After gathering the multiple children of elite stand and process March in the coming year, the rudiment branch of growth is as fringe bar, and the long 10cm of clip fringe bar, removes unnecessary blade, retains 3 wilson passionflower herbs of pinnate leaf, truncation fringe bar upper end, and 45 spend the fringe bar lower end of cutting sth. askew, angles.With yellow soil, as cutting medium, in cuttage, with 0.3% potassium permanganate solution, sterilize the previous day, then with film, cover.The CDP root-inducing powder aqueous solution and talcum powder furnishing pasty state with 800ppm, make rooting promoter, the cuttings shearing is put into after rooting promoter stirs 30s clockwise and taken out, cuttage is in matrix rapidly, the cuttage degree of depth is 2cm, after having inserted, water and drench, above seedbed, build the little shed of 80cm, cover film and shade net.After tloig-cutlage, use atomizer spray 1 time, ventilation 30min every morning; By the time after growing callus, every other day spray 1 time, ventilation 60min, sprays 1 time with the carbendazim aqueous solution for every 5 days, until take root, rooting rate is 33.3%.The multiple young branch cutting gained cuttage seeding of the elite stand that will grow up is cultivated, the April of Second Year clip branch carry out secondary cottage propagation.Cottage method is identical with said method, and rooting rate is 83%.
embodiment 2
Experimental field be positioned at scientific research nursery, forest-science academy, Guangxi.Nursery is positioned at 22 ° of 56 ' N, 108 ° of 21 ' E, height above sea level 80～100m, soil is that shale is grown the laterite forming, and belongs to moistening south subtropics monsoon climate, 20～21 ℃ of average annual temperature, 39.4 ℃ of the extreme highest temperatures, extreme lowest temperature is 1.5 ℃ of ﹣, 1 11.8 ℃ of monthly temperature, 7 27.6 ℃ of monthly temperature, average annual rainfall 1347.2mm.5 Nian Heijing of artificial culture in the garden multiple children of elite stand that grows up is processed, first December to the ripe liftoff 60cm of elite stand of black chaste tree place cutting strunk, in wound, coat Wound-healing agent.After gathering the multiple children of elite stand and process June in the coming year, the rudiment branch of growth is as fringe bar, and the long 12cm of clip fringe bar, removes unnecessary blade, retains 5 wilson passionflower herbs of pinnate leaf, truncation fringe bar upper end, and 45 spend the fringe bar lower end of cutting sth. askew, angles.With yellow soil, as cutting medium, in cuttage, with 0.5% potassium permanganate solution, sterilize the previous day, then with film, cover.The CDP root-inducing powder aqueous solution and talcum powder furnishing pasty state with 1000ppm, make rooting promoter, the cuttings shearing is put into after rooting promoter stirs 30s counterclockwise and taken out, cuttage is in matrix rapidly, the cuttage degree of depth is 3cm, after having inserted, water and drench, above seedbed, build the little shed of 80cm, cover film and shade net.After tloig-cutlage, use atomizer spray 1 time, ventilation 30min every morning; By the time after growing callus, every other day spray 1 time, ventilation 60min, sprays 1 time with the carbendazim aqueous solution for every 5 days, until take root, rooting rate is 40%.The multiple young branch cutting gained cuttage seeding of the elite stand that will grow up is cultivated, the June of Second Year clip branch carry out secondary cottage propagation.Rooting promoter used during secondary cuttage forms with the ABT root-inducing powder aqueous solution and the talcum powder furnishing pasty state of 1000ppm.Other cottage method is identical with said method, and rooting rate is 81%.
embodiment 3
Experimental field be positioned at scientific research nursery, forest-science academy, Guangxi.Nursery is positioned at 22 ° of 56 ' N, 108 ° of 21 ' E, height above sea level 80～100m, soil is that shale is grown the laterite forming, and belongs to moistening south subtropics monsoon climate, 20～21 ℃ of average annual temperature, 39.4 ℃ of the extreme highest temperatures, extreme lowest temperature is 1.5 ℃ of ﹣, 1 11.8 ℃ of monthly temperature, 7 27.6 ℃ of monthly temperature, average annual rainfall 1347.2mm.6 Nian Heijing of artificial culture in the garden multiple children of elite stand that grows up is processed, first November to the ripe liftoff 60cm of elite stand of black chaste tree place cutting strunk, in wound, coat red paint.After gathering the multiple children of elite stand and process May in the coming year, the rudiment branch of growth is as fringe bar, and the long 11cm of clip fringe bar, removes unnecessary blade, retains 4 wilson passionflower herbs of pinnate leaf, truncation fringe bar upper end, and 45 spend the fringe bar lower end of cutting sth. askew, angles.With yellow soil, as cutting medium, in cuttage, with 0.4% potassium permanganate solution, sterilize the previous day, then with film, cover.The CDP root-inducing powder aqueous solution and talcum powder furnishing pasty state with 900ppm, make rooting promoter, the cuttings shearing is put into after rooting promoter stirs 30s clockwise and taken out, cuttage is in matrix rapidly, the cuttage degree of depth is 2.5cm, after having inserted, water and drench, above seedbed, build the little shed of 80cm, cover film and shade net.After tloig-cutlage, use atomizer spray 1 time, ventilation 30min every morning; By the time after growing callus, every other day spray 1 time, ventilation 60min, sprays 1 time with the carbendazim aqueous solution for every 5 days, until take root, rooting rate is 37%.The multiple young branch cutting gained cuttage seeding of the elite stand that will grow up is cultivated, the May of Second Year clip branch carry out secondary cottage propagation.Rooting promoter used during secondary cuttage forms with the ABT root-inducing powder aqueous solution and the talcum powder furnishing pasty state of 900ppm.Other cottage method is identical with said method, and rooting rate is 82%.
embodiment 4
Experimental field be positioned at scientific research nursery, forest-science academy, Guangxi.Nursery is positioned at 22 ° of 56 ' N, 108 ° of 21 ' E, height above sea level 80～100m, soil is that shale is grown the laterite forming, and belongs to moistening south subtropics monsoon climate, 20～21 ℃ of average annual temperature, 39.4 ℃ of the extreme highest temperatures, extreme lowest temperature is 1.5 ℃ of ﹣, 1 11.8 ℃ of monthly temperature, 7 27.6 ℃ of monthly temperature, average annual rainfall 1347.2mm.6 Nian Heijing of artificial culture in the garden multiple children of elite stand that grows up is processed, first December to the ripe liftoff 60cm of elite stand of black chaste tree place cutting strunk, in wound, coat Wound-healing agent.After gathering the multiple children of elite stand and process April in the coming year, the rudiment branch of growth is as fringe bar, and the long 12cm of clip fringe bar, removes unnecessary blade, retains 4 wilson passionflower herbs of pinnate leaf, truncation fringe bar upper end, and 45 spend the fringe bar lower end of cutting sth. askew, angles.With yellow soil, as cutting medium, in cuttage, with 0.35% potassium permanganate solution, sterilize the previous day, then with film, cover.The CDP root-inducing powder aqueous solution and talcum powder furnishing pasty state with 800ppm, make rooting promoter, the cuttings shearing is put into after rooting promoter stirs 30s clockwise and taken out, cuttage is in matrix rapidly, the cuttage degree of depth is 2.8cm, after having inserted, water and drench, above seedbed, build the little shed of 80cm, cover film and shade net.After tloig-cutlage, use atomizer spray 1 time, ventilation 30min every morning; By the time after growing callus, every other day spray 1 time, ventilation 60min, sprays 1 time with the carbendazim aqueous solution for every 5 days, until take root, rooting rate is 34%.The multiple young branch cutting gained cuttage seeding of the elite stand that will grow up is cultivated, the May of Second Year clip branch carry out secondary cottage propagation.Rooting promoter used during secondary cuttage forms with the CDP root-inducing powder aqueous solution and the talcum powder furnishing pasty state of 1000ppm.Other cottage method is identical with said method, and rooting rate is 82.3%.
embodiment 5
Experimental field be positioned at scientific research nursery, forest-science academy, Guangxi.Nursery is positioned at 22 ° of 56 ' N, 108 ° of 21 ' E, height above sea level 80～100m, soil is that shale is grown the laterite forming, and belongs to moistening south subtropics monsoon climate, 20～21 ℃ of average annual temperature, 39.4 ℃ of the extreme highest temperatures, extreme lowest temperature is 1.5 ℃ of ﹣, 1 11.8 ℃ of monthly temperature, 7 27.6 ℃ of monthly temperature, average annual rainfall 1347.2mm.6 Nian Heijing of artificial culture in the garden multiple children of elite stand that grows up is processed, first December to the ripe liftoff 60cm of elite stand of black chaste tree place cutting strunk, in wound, coat red paint.After gathering the multiple children of elite stand and process May in the coming year, the rudiment branch of growth is as fringe bar, and the long 10cm of clip fringe bar, removes unnecessary blade, retains 3 wilson passionflower herbs of pinnate leaf, truncation fringe bar upper end, and 45 spend the fringe bar lower end of cutting sth. askew, angles.With yellow soil, as cutting medium, in cuttage, with 0.3% potassium permanganate solution, sterilize the previous day, then with film, cover.The CDP root-inducing powder aqueous solution and talcum powder furnishing pasty state with 800ppm, make rooting promoter, the cuttings shearing is put into after rooting promoter stirs 30s clockwise and taken out, cuttage is in matrix rapidly, the cuttage degree of depth is 2cm, after having inserted, water and drench, above seedbed, build the little shed of 80cm, cover film and shade net.After tloig-cutlage, use atomizer spray 1 time, ventilation 30min every morning; By the time after growing callus, every other day spray 1 time, ventilation 60min, sprays 1 time with the carbendazim aqueous solution for every 5 days, until take root, rooting rate is 34%.The multiple young branch cutting gained cuttage seeding of the elite stand that will grow up is cultivated, the April of Second Year clip branch carry out secondary cottage propagation.Rooting promoter used during secondary cuttage forms with the ABT root-inducing powder aqueous solution and the talcum powder furnishing pasty state of 800ppm.Other cottage method is identical with said method, and rooting rate is 82%.
Claims (3)
1. the adult elite stand of black chaste tree is answered young cuttage and seedling culture method, comprise and managing after the processing, cuttage, cuttage of multiple children processings, the collection of fringe bar, cuttings processing, seedbed and the cutting medium of the elite stand that grows up and with the operation of Miao Fanmiao, it is characterized in that: after the multiple children's processing by the adult elite stand of black chaste tree, gather tloig-cutlage in the seedbed of cutting medium is housed, by the management after cuttage, obtain black chaste tree cuttage seedling, the cultivation of gained cuttage seeding is carried out to cottage propagation after 1 year again and grow seedlings, main operational steps is as follows:
(1) the multiple children of adult elite stand processes: winter, select healthy and strong black chaste tree generation in 5 ~ 6 years year liftoff 60cm of an elite stand place cutting strunk, coat red paint or Wound-healing agent in wound;
(2) fringe bar gathers: after the coming year, two seasons of spring and summer gathered cutting strunk, the black chaste tree rudiment bar of trunk place germinating is as fringe bar;
(3) cuttings is processed: the long 10 ~ 12cm of clip fringe bar, remove unnecessary blade, and retain 3 ~ 5 wilson passionflower herbs of pinnate leaf, truncation fringe bar upper end, the fringe bar lower end of cutting sth. askew, 45 degree angles, then processes fringe bar;
(4) processing of seedbed and cutting medium: as cutting medium, cuttage is sterilized with 0.3 ~ 0.5% potassium permanganate solution the previous day, then covers with film with fresh yellow soil;
(5) the little shed of 80cm is built in cuttage: will process tloig-cutlage in matrix 4 ~ June, the cuttage degree of depth is 2 ~ 3cm, waters and drenches after having inserted above seedbed, covers film and shade net;
(6) after cuttage, manage: water or the carbendazim aqueous solution are obtained to cuttage seeding by atomizers spray cuttings;
(7) with Miao Fanmiao: cuttage seeding is cultivated, in two season of Second Year spring and summer clip branch carry out secondary cottage propagation.
2. the adult elite stand of a kind of black chaste tree according to claim 1 is answered young cuttage and seedling culture method, it is characterized in that: described processing fringe bar is with the CDP of 800 ~ 1000ppm or the ABT root-inducing powder aqueous solution and talcum powder furnishing root-growing agent, after the cuttings shearing is put into root-growing agent clockwise or stirred counterclockwise 30s, take out, cuttage is in matrix rapidly.
3. the multiple young cuttage and seedling culture method of the adult elite stand of a kind of black chaste tree according to claim 1, is characterized in that: after described cuttage, management is after tloig-cutlage, uses atomizer spray 1 time, ventilation 30min every morning; By the time after growing callus, every other day spray 1 time, ventilation 60min, sprays 1 time with the carbendazim aqueous solution for every 5 days.
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| CN103392576B (en) * | 2013-08-14 | 2014-07-16 | 广西壮族自治区林业科学研究院 | Black wattle light medium container cutting seedling method |
| CN106386353A (en) * | 2016-09-14 | 2017-02-15 | 广西壮族自治区林业科学研究院 | Seedling growing method of tender branch cutting for keteleeria cyclolepis flous |
| CN112586259A (en) * | 2020-12-14 | 2021-04-02 | 广西壮族自治区林业科学研究院 | Winter cuttage seedling raising method for sympodial bamboos for bamboo shoots |
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| Comparative study of chlorophyll content in diploid and tetraploid black wattle (Acacia mearnsii);Sadhna Mathura;《Forestry》;20060621;第79卷(第4期);全文 * |
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Inventor after: Shen Wenhui Inventor after: Jiang Ying Inventor after: Jiang Yi Inventor after: Huang Ronglin Inventor after: Chen Yunfeng Inventor after: Liu Xiu Inventor after: Hao Haikun Inventor after: Huang Zhiling Inventor after: Chen Shigui Inventor before: Shen Wenhui |
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Free format text: CORRECT: INVENTOR; FROM: SHEN WENHUI TO: SHEN WENHUI JIANG YING JIANG YI HUANG RONGLIN CHEN YUNFENG LIU XIU HAO HAIKUN HUANG ZHILING CHEN SHIGUI |
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