Summary of the invention
The object of the present invention is to provide cultivation and the quick garden building method of a kind of cuttage mulberry races, can overcome above-mentioned defect, by adopting radioinduction mode to carry out breeding, product ratio, demonstration, select that cuttage is easily taken root, leaf yield is high, the food value of leaf good and cuttage obviously forms main root, wooden hard, the food value of leaf is good, and leaf yield is high, does not need temperature control and HORMONE TREATMENT, under nature, cuttage survival rate reaches 90%.
The present invention is achieved through the following technical solutions:
A breeding method for mulberry races for cuttage, comprises the following steps:
(1) investigate and collect the Morus alba Resource Development of easily taking root: select Sri Lanka mulberry;
(2) the cuttage mulberry tree breed seed selection of easily taking root: comprise the following steps:
(2.1) radioinduction: the Sri Lanka mulberry winter bar of selecting in step (1) is used
60co-gamma-rays carries out irradiation treatment, comprises the following steps:
(2.1.1) clip, without freeze injury, without the Sri Lanka mulberry winter bar 10kg of damage by disease and insect, comprises approximately 1000 of effective hibernaculums, and 25 is a bundle, with brown paper bundling parcel;
(2.1.2) the Sri Lanka mulberry winter bar in step (2.1.1) is pressed to accumulated dose 5500 roentgens, the standard of dose rate 1100 roentgens/h is used
60co-gamma-rays Continuous irradiation 5 hours;
(2.2) selective radiation variant:
(2.2.1) obtain V
1generation: the hibernaculum of irradiation treatment in step (2.1.1) and branch are carried out respectively to grafting and cuttage, obtain generation band shape, be V without the variation branch section of leaf or bifurcated
1generation;
(2.2.2) obtain V
2generation: by the V in step (2.2.1)
1carry out simple bud grafting for branch, note erasing the primary bud-leaf on stock plant after germinateing, selecting the fine individual plant approaching with breeding goal is in the fall V
2generation;
(2.3) land for growing field crops cuttage general election: mid or late March, when ground temperature reaches more than 12 degrees Celsius, by the V obtaining in step (2.2.2)
2in generation, is carried out land for growing field crops cuttage general election, and therefrom selection possesses the good individual plant emphasis cultivation breedings of Comprehensive Traits such as high-quality, high yield, easy cuttage.
Further, for realizing better the present invention, in described step (2.2.1), obtain V
1the method in generation is in mid or late January, adopts cut skin bud grafting technology the hibernaculum of irradiation treatment is carried out to grafting winter, erases the primary bud-leaf on stock plant after germination, obtain occur banded, be V without the variation branch section of leaf or bifurcated
1generation.
Further, for realizing better the present invention, in described step (2.2.1), obtain V
1the method in generation is the winter bar of irradiation treatment to be cut into the long branch section cuttage of 30cm in the time that mid or late March ground temperature reaches more than 12 degrees Celsius, erases the primary bud-leaf on stock plant after germination, obtains generation band shape, is V without the variation branch section of leaf or bifurcated
1generation.
The present invention has proposed the quick garden building method of a kind of cuttage mulberry races on the basis with the breeding method of mulberry races in a kind of cuttage, comprise the following steps:
(4.1) fringe bar is prepared: early Febuary, the annual health of clip, be not less than 0.8cm V without damage by disease and insect, diameter
2for branch bundling, be put in wind sheltering moist place storage;
(4.2) select field planting ground: plant mulberry ground and select fertile soil, illumination abundance, is beneficial to the plot of irrigation;
(4.3) determine the specification of planting: select low dry dense planting, adopt wide-and narrow-row field planting, mu is planted 1000-1300 strains, wide row 1.33m, narrow row 0.60m, spacing in the rows 0.40-0.5 m;
(4.4) arrange field planting ground: weeding before field planting, deep ploughing are loosened the soil, and level land, and ditching or digging nest uses sufficient base fertilizer, base fertilizer soil last time covers, and the soil thickness of covering is not less than 30cm;
(4.5) by the branch section of cutting: branch is cut into the long cuttage branch of 20-25 ㎝, and inclined-plane at 45 ° is cut in branch lower end, and branch Xiang Duan is 1cm straight snips above bud, and every cuttage branch stays 5-6 bud, smoothly broken skin not of clip;
(4.6) cuttage field planting: the planting point that the cuttage branch cuttage shearing is entered to specify, ambient earth is treaded gently with pin, use sufficient normal root water, each planting point is 2-3 of equilateral triangle cuttages, and every is inserted part 20cm in soil apart from 5cm, exposes 1 bud;
(4.7) tree type is formed fast: comprise the following steps:
(4.7.1) late March, when new branch segment length is during to 25cm left and right, takes out cuttage branch section bad growing way, and each planting point only retains the plant strain growth of 1 strain robust growth, at liftoff 25cm place, young sprout excision is formed to trunk;
(4.7.2) lateral bud of plucking after the tip continues in germination and growth process, and every strain retains 24 forks and forms the first branch;
(4.7.3) when excurrent 4 branches in step (4.7.2) grow to 25cm, then its young sprout is extractd and formed the second branch, every branch of the second branch retains 2 to 3 results bars;
(4.8) fertile training management: when young sprout branch grows to 20cm in step (4.7), apply for the first time light liquid dung or urea, ratio is that 100 jin of clear water add 5 jin of liquid dungs or 2 liang of urea, mid or late August then, strengthens fertilizing amount.
Further, for realizing better the present invention, in described step (4.7.1) and step (4.7.3), pluck tip time phase difference two months.
Further, for realizing better the present invention, during described step (4.7.1) and step (4.7.3), apply fertilizer 2 times, and successively increase fertilising concentration.
Further, for realizing better the present invention, when mulberry bud is sprouted in described step (4.6), spray prevention and elimination of disease and pests medicine.
Further, for realizing better the present invention, in described step (4.7), young sprout branch sprays prevention and elimination of disease and pests medicine.
The present invention compared with prior art, has following beneficial effect:
1) the present invention is by adopting the mode batch process mulberry sapling of radiaction mutation, have advantages of aberration rate high, stably and rapidly, method is easy, can improve breeding efficiency, save and plant Sang Chengben;
2) mulberry tree that prepared by the inventive method has the advantages that high-quality, high yield, cuttage are easily taken root, and can overcome western silkworm district and grow seedlings, plants mulberry technical deficiency;
3) the present invention adopts the mulberry races that rooting ability is strong to carry out cuttage and seedling culture, and Yi Bucheng garden can be shortened and be planted mulberry seedling raise period, increases economic efficiency, and accelerates western silkworm and mulberry development.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1:
A breeding method for mulberry races for cuttage, comprises the following steps:
(1) investigate and collect the Morus alba Resource Development of easily taking root: select Sri Lanka mulberry;
(2) the cuttage mulberry tree breed seed selection of easily taking root: comprise the following steps:
(2.1) radioinduction: the Sri Lanka mulberry winter bar of selecting in step (1) is used
60co-gamma-rays carries out irradiation treatment, comprises the following steps:
(2.1.1) clip, without freeze injury, without the Sri Lanka mulberry winter bar 10kg of damage by disease and insect, comprises approximately 1000 of effective hibernaculums, and 25 is a bundle, with brown paper bundling parcel;
(2.1.2) the Sri Lanka mulberry winter bar in step (2.1.1) is pressed to accumulated dose 5500 roentgens, the standard of dose rate 1100 roentgens/h is used
60co-gamma-rays Continuous irradiation 5 hours;
(2.2) selective radiation variant:
(2.2.1) obtain V
1generation: the hibernaculum of irradiation treatment in step (2.1.1) and branch are carried out respectively to grafting and cuttage, obtain generation band shape, be V without the variation branch section of leaf or bifurcated
1generation;
(2.2.2) obtain V
2generation: by the V in step (2.2.1)
1carry out simple bud grafting for branch, after germinateing, note erasing the primary bud-leaf on stock plant, select in the fall the fine individual plant approaching with breeding goal; Mutagenesis two generations (V
2) be the maximum generation of variation, be also the critical period of selecting;
(2.3) land for growing field crops cuttage general election: mid or late March, when ground temperature reaches more than 12 degrees Celsius, by the V obtaining in step (2.2.2)
2in generation, is carried out land for growing field crops cuttage general election, and therefrom selection possesses the good individual plant emphasis cultivation breedings of Comprehensive Traits such as high-quality, high yield, easy cuttage.
Inventor successively investigates Yaan, Sichuan Province, Lushan, day complete, Yingjing, Ningnan, Laifeng County, Hubei, Hainan Island Ba Wangling, Jianfengling, national geological parks, volcanic crater, San Ya city, and the mulberry tree cuttage such as Sri Lanka repository of easily taking root.Easily take root more than 20 part of Morus alba Resource Development of Collection and conservation cuttage.More than the 20 parts of cuttages Morus alba Resource Development of easily taking root has been carried out to biological property, economic characters and rooting ability investigation, found that Sri Lanka's mulberry matter is harder, rooting ability is strong, and can form main root, cuttage survival rate is high, and resistant to diseases and insects is strong, is particularly suitable for cuttage and uses, but it is thinner that its shortcoming is blade, output is not remarkable, is expected, by means such as radiation-induced and chemical inductions, it is carried out to systematic breeding, improves its food value of leaf and output, reach high-quality, high yield, easily cuttage, the effect that high yield period is long.Within 2012, carry out
60the mutation breeding of co-gamma Rays.
Radiaction mutation is a kind of important breeding technique, and its major advantage is can batch process, variation frequency is high, stably and rapidly, method is easy.Different mulberry tree breed radiation-sensitive degree are different, generally 5000---and between 7500 roentgens, be dose,optimum, exceed 7500 roentgen's survival rates and just significantly reduce.The experiment proved that, use
60co-gamma-rays carries out Continuous irradiation 5 hours, can improve the survival rate after radiation, greatly increases the selection probability of variation branch section, improves breeding efficiency.The present invention selects by accumulated dose 5500 roentgens, and the standard of dose rate 1100 roentgens/h is used mulberry tree fringe bar and winter bar
60co-gamma-rays carries out Continuous irradiation, proves through production practices, and after radiation, survival rate can reach 78.4%, can greatly increase the selection probability of variation branch section, improves breeding efficiency.Owing to being subject to inhibition and the damage of ray, the germination rate in V1 generation is generally lower, and banded branch section, phenomenon without leaf branch section or bifurcated branch section appear in development delay, passing in time, and it is normal that shoot and leaf growth recovers gradually.
The present invention is the ground demonstration such as in river oil, Leshan in 2013, selected variant, and cuttage survival rate reaches 91%, and output is high, saves and plants 1000.00 yuan/0.067 hectare of mulberry seedling cost, possesses the feature that high-quality, high yield, cuttage are easily taken root.
Embodiment 2:
For realizing better the present invention, choose high-quality V
1for branch, the present embodiment, on the basis of embodiment 1, obtains V in described step (2.2.1)
1the method in generation is in mid or late January, adopts cut skin bud grafting technology the hibernaculum of irradiation treatment is carried out to grafting winter, erases the primary bud-leaf on stock plant after germination, obtain occur banded, be V without the variation branch section of leaf or bifurcated
1generation.Other parts of the present embodiment are identical with embodiment 1, repeat no more.
Embodiment 3:
For realizing better the present invention, choose high-quality V
1for branch, the present embodiment, on the basis of embodiment 1, obtains V in described step (2.2.1)
1the method in generation is the winter bar of irradiation treatment to be cut into the long branch section cuttage of 30cm in the time that mid or late March ground temperature reaches more than 12 degrees Celsius, erases the primary bud-leaf on stock plant after germination, obtains generation band shape, is V without the variation branch section of leaf or bifurcated
1generation.The present invention can select two kinds of methods to carry out V
1choose for branch, increase V
1for elimination factor.Other parts of the present embodiment are identical with embodiment 1, repeat no more.
Embodiment 4:
The present invention has proposed the quick garden building method of a kind of cuttage mulberry races on the basis with the breeding method of mulberry races in a kind of cuttage, comprise the following steps:
(4.1) fringe bar is prepared: early Febuary, the annual health of clip, be not less than 0.8cm V without damage by disease and insect, diameter
2for branch bundling, be put in wind sheltering moist place storage;
(4.2) select field planting ground: plant mulberry ground and select fertile soil, illumination abundance, is beneficial to the plot of irrigation;
(4.3) determine the specification of planting: select low dry dense planting, adopt wide-and narrow-row field planting, mu is planted 1000-1300 strains, wide row 1.33m, narrow row 0.60m, spacing in the rows 0.40-0.5 m;
(4.4) arrange field planting ground: weeding before field planting, deep ploughing are loosened the soil, and level land, and ditching or digging nest uses sufficient base fertilizer, base fertilizer soil last time covers, and the soil thickness of covering is not less than 30cm;
(4.5) by the branch section of cutting: branch is cut into the long cuttage branch of 20-25 ㎝, and inclined-plane at 45 ° is cut in branch lower end, and branch Xiang Duan is 1cm straight snips above bud, and every cuttage branch stays 5-6 bud, smoothly broken skin not of clip;
(4.6) cuttage field planting: the planting point that the cuttage branch cuttage shearing is entered to specify, ambient earth is treaded gently with pin, use sufficient normal root water, each planting point is 2-3 of equilateral triangle cuttages, and every is inserted part 20cm in soil apart from 5cm, exposes 1 bud;
(4.7) tree type is formed fast: comprise the following steps:
(4.7.1) late March, when new branch segment length is during to 25cm left and right, takes out cuttage branch section bad growing way, and each planting point only retains the plant strain growth of 1 strain robust growth, at liftoff 25cm place, young sprout excision is formed to trunk;
(4.7.2) lateral bud of plucking after the tip continues germination and growth, and every strain retains 24 forks and forms the first branch;
(4.7.3) when excurrent 4 branches in step (4.7.2) grow to 25cm, then its young sprout is extractd and formed the second branch, every branch of the second branch retains 2 to 3 results bars;
(4.8) fertile training management: when young sprout branch grows to 20cm in step (4.7), apply for the first time light liquid dung or urea, ratio is that 100 jin of clear water add 5 jin of liquid dungs or 2 liang of urea, mid or late August then, strengthens fertilizing amount.
Cuttage on the south the applicable Yangtze river basin of this kind, Yi Bucheng garden.The present invention adopts Yi Bucheng garden mulberry tree breed in natural situation, and cuttage survival rate can reach 90%, and the food value of leaf is excellent, and leaf yield is high, can obviously form main root, reaches degree of being practical.By adopting Yi Bucheng garden method, can adapt to the needs of current silkworm and mulberry production development, be the fast province measure of increasing economic efficiency.
Embodiment 5:
For realizing better the present invention, guarantee young sprout branch normal growth, in described step (4.7.1) and step (4.7.3), pluck tip time phase difference two months.Other parts of the present embodiment are identical with embodiment 4, repeat no more.
Embodiment 6:
For realizing better the present invention, guarantee young sprout branch normal growth, during described step (4.7.1) and step (4.7.3), apply fertilizer 2 times, and successively increase fertilising concentration.By to young sprout split application, guarantee mulberry yield.Other parts of the present embodiment are identical with embodiment 4, repeat no more.
Embodiment 7:
For realizing better the present invention, the present embodiment, on the basis of embodiment 4, sprays prevention and elimination of disease and pests medicine when mulberry bud is sprouted in described step (5.6).While sprouting for anti-last feeding bud, there is mulberry small weevil, mulberry geometrid (Hemerophila atrilineata) bud-leaf pest damage mulberry bud, therefore can be in step (4.6) adopt conventional extermination of disease and insect pest medicine to spray cuttage plant, prevention and elimination of disease and pests when the sprouting of mulberry bud.Other parts of the present embodiment are identical with embodiment 4, repeat no more.
Embodiment: 8:
For realizing better the present invention, the present embodiment is on the basis of embodiment 4, and in described step (4.7), young sprout branch sprays prevention and elimination of disease and pests medicine.In order to prevent mulberry small weevil, mulberry geometrid (Hemerophila atrilineata) bud-leaf pest damage young sprout branch, therefore can adopt extermination of disease and insect pest medicine to spray cuttage plant, prevention and elimination of disease and pests by young sprout in step (4.7).Other parts of the present embodiment are identical with embodiment 4, repeat no more.
The above, be only preferred embodiment of the present invention, not the present invention done to any pro forma restriction, and any simple modification, equivalent variations that every foundation technical spirit of the present invention is done above embodiment, within all falling into protection scope of the present invention.