CN106105988B - Seedling growing method for Tinospora yunnanensis - Google Patents
Seedling growing method for Tinospora yunnanensis Download PDFInfo
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- CN106105988B CN106105988B CN201610530728.4A CN201610530728A CN106105988B CN 106105988 B CN106105988 B CN 106105988B CN 201610530728 A CN201610530728 A CN 201610530728A CN 106105988 B CN106105988 B CN 106105988B
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- 238000003306 harvesting Methods 0.000 claims abstract description 7
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
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- 238000005507 spraying Methods 0.000 claims description 13
- 239000003337 fertilizer Substances 0.000 claims description 12
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 10
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 10
- 239000006013 carbendazim Substances 0.000 claims description 10
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- NNKVPIKMPCQWCG-UHFFFAOYSA-N methamidophos Chemical compound COP(N)(=O)SC NNKVPIKMPCQWCG-UHFFFAOYSA-N 0.000 claims description 2
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- SUABMOWIQWUPPE-UHFFFAOYSA-N dihydrogen phosphate;methylazanium Chemical compound NC.OP(O)(O)=O SUABMOWIQWUPPE-UHFFFAOYSA-N 0.000 description 1
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- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a seedling raising method of radix tinosporae Yunnanensis, which comprises the following steps: seed treatment; selecting a nursery land and preparing a bed; accelerating germination; transplanting; managing the nursery stocks; and (6) harvesting the fruits. Through research and development of a seed breeding technology of the Tinospora yunnanensis, the germination rate of the Tinospora yunnanensis seeds reaches 85-90%, and the seedling raising technology is economical, efficient and feasible. The artificial seedling raising is realized on the premise of protecting the existing Tinospora yunnanensis resources. Meanwhile, the invention of the Tinospora yunnanensis seed seedling raising technology also adds a new way for artificial breeding of Tinospora yunnanensis, so that genes of the Tinospora yunnanensis are more effectively stored and expanded, and a good foundation is laid for regression afforestation planting and population quantity expansion of the Tinospora yunnanensis.
Description
Technical Field
The invention belongs to the technical field of seed seedling culture, and particularly relates to a seedling culture method of Tinospora yunnanensis.
Background
According to the verification, the seedling raising technology of the Tinospora yunnanensis seeds at home and abroad is not reported, and only the seedling raising technology of the Tinospora yunnanensis by cutting is reported.
The cutting seedling raising of the Tinospora yunnanensis is generally carried out according to the following scheme:
1) preparing a seedbed: cleaning weeds on a seedbed with the width of 90cm and the width of a footpath of 30cm, wherein the seedbed is 20cm higher than the footpath, raking and cutting soil, and watering thoroughly for later use.
2) Ear harvesting: cutting propagation is carried out by adopting panicle of Tibet olive. Collecting ear strips, sterilizing with 500 times of carbendazim for 30min, and washing with clear water.
3) Cuttage: shearing the panicle of Tinospora Yunnanensis into saddle shape, soaking the sheared edge in gibberellin solution of 0.1g/L for 10min, and cutting while treating.
4) Heat preservation and moisture preservation: after the cutting of the cutting slips is finished, a plastic film is covered on a small arched shed supported by bamboo chips, the temperature is kept at 25-35 ℃, and the humidity is kept at 75-85%.
5) And (3) cutting management: watering, weeding, sprout removing, and inspecting the callus and rooting condition of the cutting.
6) Lifting the film: after the cutting shoots and leaves grow, the cutting shoots grow to about 10cm, the film is lifted to 10:00 film covering of the next day after 18:00 every night, the operation is repeated for about 10 days, and the film is completely lifted according to the weather conditions.
The cutting propagation is to take part of the plant's nutritive organ, insert it into loose and wet soil or fine sand, and take root and branch by using its regeneration capacity to grow new plant. The Tinospora yunnanensis is bred by adopting a cutting propagation mode, the length of a cutting shoot is about 10cm generally, the demand for scion cuttings of the Tinospora yunnanensis is relatively large, but the quantity of the existing Tinospora yunnanensis is small, and the artificial breeding of the Tinospora yunnanensis is limited to a certain extent.
Disclosure of Invention
In view of the above, the invention provides a seedling raising method for the Tinospora yunnanensis, which is used for collecting seeds of the Tinospora yunnanensis in the field and carrying out sowing and seedling raising exploration in the current situation that only 6 Tinospora yunnanensis are stored in the field, and through research on seedling raising technology of the Tinospora yunnanensis seeds, a relatively economic and rapid seedling raising mode of the Tinospora yunnanensis under the current situation is sought, so that the method has great significance in expanding propagation and protecting the very small species of the Tinospora yunnanen.
In order to solve the technical problem, the invention discloses a seedling raising method of radix tinosporae Yunnanensis, which comprises the following steps:
1) seed treatment:
after the fruit of the Tinospora yunnanensis is mature in shape, the physiological after-ripening period is 10-15 d, and the germination rate of direct sowing is low; placing the collected Tinospora yunnanensis fruits in a ventilating and shady place for 10-15 days to naturally finish physiological after-ripening, extruding seeds, soaking the stripped seeds in a carbendazim solution with the concentration of 400 times for 1 hour, rinsing the seeds by using clear water, and waiting for sowing;
2) selecting a nursery land and preparing a bed:
selecting loam with flat terrain, fertile soil, good drainage and ventilation as a nursery land; after a nursery land is selected, deep ploughing is carried out, the depth is 25-30 cm, the soil is sprayed with 500 times of potassium permanganate for disinfection, the soil is exposed to the sun for 2 weeks, and after the soil disinfection is finished, a nutrition bag with the specification of 16cm multiplied by 20cm is filled with the soil for standby; building a sunshade net on the nursery land; river sand is used as a substrate of the germination accelerating beds, the germination accelerating beds are arranged below a sunshade net, the width of each bed is 1m, the height of each bed is 15-20 cm, the length of each bed is adjusted according to the terrain, and the distance between every two germination accelerating beds is 50 cm;
3) accelerating germination:
dibbling the treated Tinospora yunnanensis seeds on a germination accelerating bed according to the specification of plant-row spacing of 3cm multiplied by 3cm, covering sawdust with the thickness of 4-5 cm on the seeds after dibbling, and watering thoroughly; after 15 days of sowing, the seeds begin to germinate, and the germination rate of the Tinospora yunnanensis seeds is 85-90%;
4) transplanting:
transplanting the seedlings when the seedlings grow to 5-8 cm on a germination accelerating bed; the planting depth is determined according to the root length, and the root is not indented below the planting depth, and the ground diameter mark is not exposed above the planting depth; meanwhile, the arrangement direction of the cotyledons is kept consistent to ensure sufficient light reception; the whole operation process is carried out in a shading environment; immediately after transplanting, thoroughly watering the substrate by 1000 times of carbendazim at one time;
5) and (3) seedling management:
5.1) water, fertilizer and weeding management:
the water and fertilizer management is required for the growth of the Tinospora yunnanensis in the seedling stage, the water spraying in the seedling stage is not excessive, the water shortage is not caused, the growth is difficult to recover or the death is caused once the branches and leaves are dry, and the relative humidity of the soil in the nutrition bag is ensured to be 60-70%; dressing 1g of compound fertilizer and urea every 10 days after the growth of the seedlings is recovered for 30 days, wherein the using amount is gradually increased along with the growth of the seedlings, and the using amount is not more than 2g per plant at most; weeds are removed in time, fertilizer, water, light and heat are prevented from being strived for by the weeds and the seedlings, and normal growth of the seedlings is facilitated;
5.2) pest control
After the bud seedlings are transplanted to survive, alternately spraying the boldo liquid and the carbendazim liquid for 1 time every month; in the seedling raising process, the leaves are rotted and should be removed to properly reduce the moisture; spraying to kill pests; in addition, the periphery of the nursery garden is kept neat without stacking sundries and grass piles;
5.3) hardening seedlings:
hardening seedlings 1-2 months before the seedlings leave the nursery, strictly controlling the illumination time at the initial stage of hardening seedlings, uncovering a sunshade net at 18:00 hours every day, covering at 11:00 hours, and gradually increasing the illumination time and intensity after repeating for one week; during hardening off, the fertilization is stopped to improve the stress resistance of the seedlings, the lignification of the seedlings can be promoted through hardening off, the capability of resisting diseases and insect pests is enhanced, and the survival rate of the forestation after the seedlings are out of the nursery is improved;
5.4) outplanting the nursery;
6) harvesting fruits:
the flowering period of the Tibet olive is 8-9 months, and the fruit is ripe in the next year in 1-4 months; when the fruit is ripe, the peel is changed from smooth and hard to rough, wrinkled and soft; the seed coat is changed from tender and milky white to hard and yellow brown; after the fruit of the Tinospora yunnanensis is mature in shape, the physiological after-ripening period is 10-15 days; placing the collected Tinospora yunnanensis fruits in a ventilating shade place for 10-15 days to naturally finish physiological after-ripening; when the fruits become soft, extruding the seeds for standby; 10-29 g of single Tinospora yunnanensis fruit, and 1-4 seeds are contained in each fruit.
Further, the PH value of the soil of the nursery land in the step 2) is 6.5-7.0; the shading degree of the shading net is 95 percent.
Further, the humidity of the sawdust in the step 3) is 70-80%, and the temperature is 23-25 ℃.
Further, the spraying insecticide in the step 5.2) is specifically as follows: spraying mole cricket and cutworm underground pests with 50% methamidophos emulsifiable solution in an amount of l 000-2000 times; spraying aphids with 1500-2000 times of imidacloprid; lepidoptera pests are sprayed with 1000-1500 times of liquid of beta-cypermethrin.
Further, the conditions for outplanting the nursery stock in the step 5.4) are as follows: when the height of the Yunnan Tibet olive seedlings is more than or equal to 30cm and the ground diameter is more than or equal to 0.25cm, the Yunnan Tibet olive seedlings are outplanted and forested.
Compared with the prior art, the invention can obtain the following technical effects:
1) through research and development of a seed breeding technology of the Tinospora yunnanensis, the germination rate of the Tinospora yunnanensis seeds reaches 85-90%, and the seedling raising technology is economical, efficient and feasible.
2) The artificial seedling raising is realized on the premise of protecting the existing Tinospora yunnanensis resources.
3) The invention of the Tinospora yunnanensis seed seedling raising technology also adds a new way for artificial breeding of Tinospora yunnanensis, so that genes of Tinospora yunnanensis are more effectively stored and expanded, and a good foundation is laid for regression afforestation planting and population quantity expansion of Tinospora yunnanensis.
Of course, it is not necessary for any one product in which the invention is practiced to achieve all of the above-described technical effects simultaneously.
Detailed Description
The following embodiments are described in detail with reference to the accompanying drawings, so that how to implement the technical features of the present invention to solve the technical problems and achieve the technical effects can be fully understood and implemented.
The invention provides a seedling raising method of radix tinosporae Yunnanensis, which comprises the following steps:
1) seed treatment
After the fruit of the Tinospora yunnanensis is mature in shape, the physiological after-ripening period is 10-15 d, and the germination rate of direct sowing is low; placing the collected Tinospora yunnanensis fruits in a ventilating and shady place for 10-15 days to enable the Tinospora yunnanensis fruits to naturally finish physiological after-ripening, extruding the seeds, soaking the stripped seeds in a carbendazim solution with the concentration of 400 times for 1 hour, rinsing the seeds by using clear water, and waiting for sowing.
2) Nursery selection and bed preparation
Selecting loam with flat terrain, fertile soil, good drainage, ventilation and pH of 6.5-7.0 as a nursery land. After a nursery land is selected, deep ploughing is carried out, the depth is 25-30 cm, the soil is sprayed with 500 times of potassium permanganate for disinfection, the soil is exposed to the sun for 2 weeks, and after the soil disinfection is finished, a nutrition bag with the specification of 16cm multiplied by 20cm is filled with the soil for standby. And building a sunshade net with the shading degree of 95% on the nursery land.
River sand is used as a substrate of the germination accelerating beds, the germination accelerating beds are arranged under a sunshade net, the width of each bed is 1m, the height of each bed is 15-20 cm, the length of each bed is adjusted according to the terrain, and the distance between every two germination accelerating beds is 50 cm.
3) Pregermination treatment
Dibbling the treated Tinospora yunnanensis seeds on a germination accelerating bed according to the specification of plant spacing of 3cm multiplied by 3cm, covering sawdust with the thickness of 4-5 cm on the seeds after dibbling, and thoroughly watering to keep the humidity of the sawdust at 70-80% and the temperature at 23-25 ℃. After 15 days of sowing, the seeds begin to germinate, and the germination rate of the Tibet olive seeds is 85-90%.
4) Transplanting
And transplanting the seedlings when the seedlings grow to 5-8 cm on the germination accelerating bed. The depth of planting is determined according to the root length, and the root is not indented below and the spot and the radial mark are not exposed above. Meanwhile, the arrangement direction of the cotyledons is kept consistent as much as possible so as to ensure sufficient light receiving. The whole operation process is carried out in a shading environment, otherwise, the seedlings can be withered due to water shortage, and the later growth and development of the seedlings are greatly influenced. Immediately after transplanting, the substrate is thoroughly watered with 1000 times of carbendazim at one time to stabilize the root system and avoid infection caused by seedling transplantation.
5) Nursery stock management
5.1) management of Water, Fertilizer and weed control
Water and fertilizer management is required for the growth of the Tinospora yunnanensis in the seedling stage, water spraying in the seedling stage is not excessive, water shortage is not caused, growth is difficult to recover or death is caused once branches and leaves are dry, and the relative humidity of soil in the nutrition bag is ensured to be 60-70%. And (3) dressing 1g of compound fertilizer and urea every 10 days after the seedlings recover to grow 30 days, wherein the dosage is gradually increased along with the growth of the seedlings, and the maximum dosage is not more than 2g per plant. Weeds should be removed in time, so that fertilizer, water, light and heat are prevented from being strived for by the weeds and seedlings, and normal growth of the seedlings is facilitated.
5.2) pest control
After the bud seedlings are transplanted to survive, the Boldoer solution and the carbendazim solution are alternately sprayed for 1 time per month. In the process of seedling cultivation, if leaf rot occurs, the leaf rot should be removed to reduce water content properly. The underground pests such as mole cricket, cutworm and the like can be sprayed by 50 percent methylamine phosphate emulsion l 000-2000 times; the aphids can be sprayed by imidacloprid with 1500-2000 times of liquid; lepidoptera pests can be sprayed by 1000-1500 times of liquid of beta-cypermethrin; in addition, the periphery of the nursery garden should be kept clean and tidy without stacking sundries, grass piles and the like.
5.3) hardening off the seedlings
Hardening seedlings 1-2 months before seedlings leave the nursery, strictly controlling illumination time in the early stage of hardening seedlings, uncovering a sunshade net at 18:00 hours every day, covering at 11:00 hours, and gradually increasing the illumination time and intensity after repeating for one week. During hardening off, the fertilization is stopped to improve the stress resistance of the seedlings, the lignification of the seedlings can be promoted through hardening off, the capability of resisting diseases and insect pests is enhanced, and the survival rate of the forestation out of the nursery is improved.
5.4) nursery of the Nursery
When the height of the Yunnan Tibet olive seedlings is more than or equal to 30cm and the ground diameter is more than or equal to 0.25cm, the Yunnan Tibet olive seedlings can be outplanted and forested.
6) Harvesting of fruits
The flowering period of the Tibet olive is 8-9 months, and the fruit is ripe in the next year in 1-4 months. When the fruit is ripe, the peel is changed from smooth and hard to rough, wrinkled and soft; the seed coat changes from tender and milky white to hard and yellowish brown. After the fruit of the Tinospora yunnanensis is mature in shape, the physiological post-maturation period is 10-15 days. And placing the collected Tibet olive fruits in a ventilating shade place for 10-15 days to naturally finish physiological after-ripening. And when the fruits become soft, extruding the seeds for standby. 10-29 g of single Tinospora yunnanensis fruit, and 1-4 seeds are contained in each fruit.
The fruit stage of yunnan olive is not fixed, the fruit is generally started from 10 months per year to 2 months next year, and the mature period is usually 1 to 6 months. Because the fruiting time is not fixed, the fruit ripening period of the same tree 1 can be different from that of the same tree 1 for 2 times by 3-5 months, in the actual harvesting process, observation is carried out from 10 months, 1 time per month until the fruit of the Tinospora yunnanensis is found, and whether the seed is ripe can be observed at intervals of 3 months after the fruit is found. When the Tinospora yunnanensis fruit is changed from smooth and hard to slightly wrinkled and soft and the seeds are changed from tender and milky white to hard and yellow brown, the seeds can be extruded by slight force, and at the moment, the seeds are mature and can be collected.
In 2013, in 5 months and 7 months, forestry technicians in rui city develop two-stage artificial cuttage propagation-expansion tests of the Tinospora yunnanensis in the rui rare vegetation garden. The test result shows that the 60 tibetan olive cuttings in the cutting test have 48 rooted and hairy leaves, and the cutting survival rate is 80%. The survival of the Tibet olive cutting slips marks the success of artificial propagation expansion of the special wild Tibet olive plant of Yunnan province.
The harvesting time of the invention is crucial to the success of the artificial propagation of the Tinospora yunnanensis, the fruits of the Tinospora yunnanensis are usually ripe within 1 to 6 months according to the difference of fruit bearing time, the fruits are changed from smooth and hard to slightly wrinkled and soft, the seeds are changed from tender and milky white to hard and yellowish brown, the seeds can be harvested when being extruded with slight force, the seeds are not completely developed in early picking, the germination rate is low, the seedlings are weak in growth, and the seeds fall off or are gnawed by birds and beasts. Secondly, breaking physiological after-ripening: the collected Tinospora yunnanensis seeds and fruit peels are placed in a ventilated and non-illuminated room for 12-15 days, the fruits cannot be completely mature in less than 12 days, so that the germination rate can be reduced or seedlings grow slowly, and the seeds easily mildew in more than 15 days, so that the germination rate is seriously influenced. And thirdly, controlling the germination accelerating temperature and humidity, wherein the humidity is kept at 70-80%, the temperature is kept at 22-25 ℃, the germination of the seeds needs proper temperature and humidity, the humidity is lower than 70%, the temperature is lower than 22 ℃, the germination rate of the seeds is reduced, the humidity is higher than 80%, and the seeds are easy to mildew and seriously reduce the germination rate when the temperature exceeds 25 ℃. And fourthly, transplanting seedlings when the transplanting height of the seedlings is 5-8 cm, wherein stem leaves are tender when the transplanting height of the seedlings is less than 5cm, the seedlings are easy to break or damage during transplanting, the roots of the seedlings transplanted above 8cm are too long and easy to nest, the seedlings need to be pruned, and the seedlings can be recovered to grow after being transplanted in a long time.
While the foregoing description shows and describes several preferred embodiments of the invention, it is to be understood, as noted above, that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. A seedling raising method of Tibet olive is characterized by comprising the following steps:
1) seed treatment:
after the fruit of the Tinospora yunnanensis is mature in shape, the physiological after-ripening period is 10-15 d, and the germination rate of direct sowing is low; placing the collected Tinospora yunnanensis fruits in a ventilating and shady place for 10-15 days to naturally finish physiological after-ripening, extruding seeds, soaking the stripped seeds in a carbendazim solution with the concentration of 400 times for 1 hour, rinsing the seeds by using clear water, and waiting for sowing;
2) selecting a nursery land and preparing a bed:
selecting loam with flat terrain, fertile soil, good drainage and ventilation as a nursery land; after a nursery land is selected, deep ploughing is carried out, the depth is 25-30 cm, the soil is sprayed with 500 times of potassium permanganate for disinfection, the soil is exposed to the sun for 2 weeks, and after the soil disinfection is finished, a nutrition bag with the specification of 16cm multiplied by 20cm is filled with the soil for standby; building a sunshade net on the nursery land; river sand is used as a substrate of the germination accelerating beds, the germination accelerating beds are arranged below a sunshade net, the width of each bed is 1m, the height of each bed is 15-20 cm, the length of each bed is adjusted according to the terrain, and the distance between every two germination accelerating beds is 50 cm;
3) accelerating germination:
dibbling the treated Tinospora yunnanensis seeds on a germination accelerating bed according to the specification of plant-row spacing of 3cm multiplied by 3cm, covering sawdust with the thickness of 4-5 cm on the seeds after dibbling, and watering thoroughly; after 15 days of sowing, the seeds begin to germinate, and the germination rate of the Tibet olive seeds is 85-90%;
4) transplanting:
transplanting the seedlings when the seedlings grow to 5-8 cm on a germination accelerating bed; the planting depth is determined according to the root length, and the root is not indented below the planting depth, and the ground diameter mark is not exposed above the planting depth; meanwhile, the arrangement direction of the cotyledons is kept consistent to ensure sufficient light reception; the whole operation process is carried out in a shading environment; immediately after transplanting, thoroughly watering the substrate by 1000 times of carbendazim at one time;
5) and (3) seedling management:
5.1) water, fertilizer and weeding management:
the water and fertilizer management is required for the growth of the Tinospora yunnanensis in the seedling stage, the water spraying in the seedling stage is not excessive, the water shortage is not caused, the growth is difficult to recover or the death is caused once the branches and leaves are dry, and the relative humidity of the soil in the nutrition bag is ensured to be 60-70%; dressing 1g of compound fertilizer and urea every 10 days after the growth of the seedlings is recovered for 30 days, wherein the using amount is gradually increased along with the growth of the seedlings, and the using amount is not more than 2g per plant at most; weeds are removed in time, fertilizer, water, light and heat are prevented from being strived for by the weeds and the seedlings, and normal growth of the seedlings is facilitated;
5.2) pest control
After the bud seedlings are transplanted to survive, alternately spraying the boldo liquid and the carbendazim liquid for 1 time every month; in the seedling raising process, the leaves are rotted and should be removed to properly reduce the moisture; spraying to kill pests; in addition, the periphery of the nursery garden is kept neat without stacking sundries and grass piles;
5.3) hardening seedlings:
hardening seedlings 1-2 months before the seedlings leave the nursery, strictly controlling the illumination time at the initial stage of hardening seedlings, uncovering a sunshade net at 18:00 hours every day, covering at 11:00 hours, and gradually increasing the illumination time and intensity after repeating for one week; during hardening off, the fertilization is stopped to improve the stress resistance of the seedlings, the lignification of the seedlings can be promoted through hardening off, the capability of resisting diseases and insect pests is enhanced, and the survival rate of the forestation after the seedlings are out of the nursery is improved;
5.4) outplanting the nursery;
6) harvesting fruits:
the flowering period of the Tibet olive is 8-9 months, and the fruit is ripe in the next year in 1-4 months; when the fruit is ripe, the peel is changed from smooth and hard to rough, wrinkled and soft; the seed coat is changed from tender and milky white to hard and yellow brown; after the fruit of the Tinospora yunnanensis is mature in shape, the physiological after-ripening period is 10-15 days; placing the collected Tinospora yunnanensis fruits in a ventilating shade place for 10-15 days to naturally finish physiological after-ripening; when the fruits become soft, extruding the seeds for standby; 10-29 g of single Tinospora yunnanensis fruit, and 1-4 seeds are contained in each fruit.
2. A seedling raising method of tinospora yunnanensis according to claim 1, wherein the PH of the soil in the nursery land in the step 2) is 6.5 to 7.0; the shading degree of the shading net is 95 percent.
3. A seedling raising method of the tinospora yunnanensis of claim 1, wherein the humidity of the sawdust in the step 3) is 70-80%, and the temperature is 23-25 ℃.
4. The seedling raising method of tinospora yunnanensis as claimed in claim 1, wherein the spraying in step 5.2) is to kill insects, specifically: spraying mole cricket and cutworm underground pests with 50% methamidophos emulsifiable solution in an amount of l 000-2000 times; spraying aphids with 1500-2000 times of imidacloprid; lepidoptera pests are sprayed with 1000-1500 times of liquid of beta-cypermethrin.
5. A seedling raising method of tinospora yunnanensis according to claim 1, wherein the conditions for nursery of the nursery trees in the step 5.4) are: when the height of the Yunnan Tibet olive seedlings is more than or equal to 30cm and the ground diameter is more than or equal to 0.25cm, the Yunnan Tibet olive seedlings are outplanted and forested.
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