CN102668858B - Seedling cultivating method for cutting hard woods of acer palmatum - Google Patents
Seedling cultivating method for cutting hard woods of acer palmatum Download PDFInfo
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- CN102668858B CN102668858B CN201210181910.5A CN201210181910A CN102668858B CN 102668858 B CN102668858 B CN 102668858B CN 201210181910 A CN201210181910 A CN 201210181910A CN 102668858 B CN102668858 B CN 102668858B
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Abstract
The invention discloses a seedling cultivating method for cutting hard woods of acer palmatum. The method comprises the following steps: (1) selecting an appropriate cutting time; (2) preparing a cutting substrate; (3) preparing rooting agent; (4) cutting slips processing and cutting; and (4) managing and transplanting after cutting. The seedling cultivating method for cutting the hard woods of the acer palmatum, disclosed by the invention, has the characteristics as follows: the cutting survival rate reaches more than 80%; compared with the cost of acer palmatum softwood cuttage, the cost of hard wood cuttage is reduced by 30-50%; the cost of hard wood cuttage is only about 20% of the cost of a tissue culturing method; and compared with a seedling cultivating period of the acer palmatum graftage, the seedling cultivating period of the hard wood cuttage is reduced by 2-3 years. The method disclosed by the invention has the advantages of simplicity, convenience and easiness in operation, low cost, simple technical requirements, easiness for promotion and application as well as important application and promotion values in acer palmatum seedling breeding production.
Description
Technical field
The present invention relates to a Plants asexual reproduction method, specifically relate to a kind of Acer palmatum hardwood cutting and seedling raising method.
Background technology
Acer palmatum (Acer palmatum) is Aceraceae (Aceraceae) Acer (Acer) deciduous tree or dungarunga, originates in each province, China Yangtze river basin, in Japan, Korea S etc., also has distribution.Acer palmatum tree performance is graceful, leaf peculiar, and in good day of spring and autumn, color leaf gardenful, is world-renowned foliage tree kind.Acer palmatum garden-variety is various, and afforestation application is very extensive.
The vegetative propagation of plant can keep maternal good characteristic, the cultivation in a organized way of the asexual reproduction method of Acer palmatum, grafting and cuttage etc.Wherein, tissue culture technique requires high, complicated operation, and production cost is expensive; Biennial above Acer palmatum stock need to be first cultivated in propagation by grafiting, then required Acer palmatum kind scion is grafted on stock to the cultivating seedlings phase that therefore need to be longer; There is in recent years scholar to carry out the test of Acer palmatum softwood cutting propagation, the honest summer high temperature of epicormic branch cuttage period, need to possess full exposure spraying system device can implement, and the method has greatly limited seedling manufacturing enterprise and the peasant household of growing seedlings carries out breeding on a large scale of Acer palmatum seedling.The cuttage implementation condition of Acer palmatum is easy, processing ease, and reproductive-cost is lower; By literature search, the domestic report that there is not yet.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of simple to operate, with low cost, Acer palmatum hardwood cutting and seedling raising method that reproductive efficiency is high.
Technical scheme: the present invention is achieved through the following technical solutions:
An Acer palmatum hardwood cutting and seedling raising method, is characterized in that, comprises the following steps:
(1) cutting time: be advisable before Acer palmatum rudiment in spring foliation;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into cutting medium by the volume ratio of 1: 2～1: 3, with 50% carbendazol wettable powder, dilute 800～1000 times of medicine liquid sprays cutting mediums of sterilizing;
(3) preparation root-growing agent: by indolebutyric acid, manganese sulphate, Cobastab
1be mixed in proportion, standby;
(4) prepare cuttings: select Acer palmatum tree crown middle and upper part 1 year raw healthy and strong, lateral bud is full, without the branch of damage by disease and insect.
Clip length is 8～12cm, and the branch that diameter is 0.3～0.8cm is as cuttings, and the upper and lower clip of cutting is truncate, and upper clip is apart from clip bud 0.5～1cm, and clip is level and smooth, and every root cutting will have 2～4 full buds;
(5) cuttings is processed and cuttage: after the base portion of cuttings is soaked respectively in clear water, liquor potassic permanganate, insert in the root-growing agent that step (3) prepares and soak, water permeable after the cutting medium that inserting step (2) configures;
(6) insert after management with transplant: the cuttings of cuttage in step (4) is placed in to plastic canopy insulation, moisturizing, wherein encircles temperature of shed and remain on 25 ℃ of left and right, relative moisture reaches more than 80%; After cuttage, every 10～15d, spray 500～800 times of 50% carbendazol wettable powder dilution thimerosals 1 time, prevent from going rotten, remove in time rotten cuttings; After 20～30 days, cuttings base portion starts to form callus, in the time of 40～60 days, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root, within 70～80 days, transplants afterwards.
2, Acer palmatum hardwood cutting and seedling raising method according to claim 1, is characterized in that, the cutting time described in step (1) is that mid-March in spring is to by the end of March;
3, Acer palmatum hardwood cutting and seedling raising method according to claim 1, is characterized in that, the root-growing agent described in step (3) be indolebutyric acid 200～400mg be dissolved in 20～40ml 95% ethanol in, add manganese sulphate 20～30mg, Cobastab
1after 3.0～5.0mg, add water to 1kg, after mixing, make;
4, Acer palmatum hardwood cutting and seedling raising method according to claim 1, it is characterized in that, it is that the base portion of cuttings was soaked after 6～12 hours in clear water that cuttings described in step (5) is processed, with dipping 1～2min in 0.5% liquor potassic permanganate, insert in the root-growing agent that step (3) prepares and soak 1～2 hour.
Beneficial effect: Acer palmatum hardwood cutting and seedling raising method of the present invention, cuttage survival rate reaches more than 80%, compared with the cost of Acer palmatum epicormic branch cuttage 30%～50%; It is only method for tissue culture cost 20% left and right; Compare with Acer palmatum propagation by grafiting, shorten growing-seedling period 2～3 years.This method is easy to operation, has that cost is low, specification requirement is simple, the advantage such as easily applies, and on Acer palmatum sapling multiplication is produced, has important application and promotional value.
Embodiment
Embodiment 1
(1) cutting time: mid-March in spring is to Acer palmatum by the end of March not yet during rudiment;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into cutting medium by the volume ratio of 1: 2, with 50% carbendazol wettable powder, dilute 800 times of medicine liquid sprays cutting mediums of sterilizing;
(3) preparation root-growing agent: indolebutyric acid 200mg is dissolved in 95% the ethanol of 20ml, adds manganese sulphate 20mg, Cobastab
1after 3.0mg, add water to 1kg, after mixing, make root-growing agent, standby;
(4) prepare cuttings: select Acer palmatum tree crown middle and upper part 1 year raw healthy and strong, lateral bud is full, without the branch of damage by disease and insect.Clip length is 8cm, and the branch that diameter is 0.3cm is as cuttings, and the upper and lower clip of cutting is truncate, and upper clip is apart from clip bud 0.5～1cm, and clip is level and smooth, and every root cutting has 2～4 full buds.
(5) cuttings is processed and cuttage: the base portion of cuttings was soaked after 6 hours in clear water, with dipping 1min in 0.5% liquor potassic permanganate, insert in the root-growing agent that step (3) prepares and soak 2 hours, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert rear management and transplant: the cuttings being plugged in step (4) is placed in to plastic canopy insulation, moisturizing, wherein encircles temperature of shed and remain on 25 ℃ of left and right, canopy reaches more than 80% humidity; After cuttage, every 10～15d, spray 500～800 times of 50% carbendazol wettable powder dilution thimerosals 1 time, prevent from going rotten, remove in time rotten cuttings; After 30 days, cuttings base portion starts to form callus, in the time of 60 days, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root, within 80 days, transplants afterwards, and cuttage survival rate reaches 83%.
Embodiment 2
(1) cutting time: in extremely not yet rudiment of Acer palmatum by the end of March mid-March in spring;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into cutting medium by the volume ratio of 1: 2, with 50% carbendazol wettable powder, dilute 800 times of medicine liquid sprays cutting mediums of sterilizing;
(3) preparation root-growing agent: indolebutyric acid 400mg is dissolved in 95% the ethanol of 40ml, adds manganese sulphate 30mg, Cobastab
1after 5.0mg, add water to 1kg, after mixing, make root-growing agent, standby;
(4) prepare cuttings: select Acer palmatum tree crown middle and upper part 1 year raw healthy and strong, lateral bud is full, without the branch of damage by disease and insect.Clip length is 10cm, and the branch that diameter is 0.5cm is as cuttings, and the upper and lower clip of cutting is truncate, and upper clip is apart from clip bud 0.5～1cm, and clip is level and smooth, and every root cutting has 2～4 full buds;
(5) cuttings is processed and cuttage: the base portion of cuttings was soaked after 12 hours in clear water, with dipping 2min in 0.5% liquor potassic permanganate, insert in the root-growing agent that step (3) prepares and soak 1 hour, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert rear management and transplant: the cuttings being plugged in step (4) is placed in to plastic canopy insulation, moisturizing, wherein encircles temperature of shed and remain on 25 ℃ of left and right, relative moisture reaches more than 80%; After cuttage, every 10～15d, spray 500～800 times of 50% carbendazol wettable powder dilution thimerosals 1 time, prevent from going rotten, remove in time rotten cuttings; After 25 days, cuttings base portion starts to form callus, about 50 days, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root, within 80 days, transplants afterwards, and cuttage survival rate reaches 86%
Embodiment 3
(1) cutting time: in extremely not yet rudiment of Acer palmatum by the end of March mid-March in spring;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into cutting medium by the volume ratio of 1: 3, with 50% carbendazol wettable powder, dilute 1000 times of medicine liquid sprays cutting mediums of sterilizing;
(3) preparation root-growing agent: indolebutyric acid 300mg is dissolved in 95% the ethanol of 30ml, adds manganese sulphate 25mg, Cobastab
1after 4.0mg, add water to 1kg, after mixing, make root-growing agent, standby;
(4) prepare cuttings: select Acer palmatum tree crown middle and upper part 1 year raw healthy and strong, lateral bud is full, without the branch of damage by disease and insect.Clip length is 12cm, and the branch that diameter is 0.8cm is as cuttings, and the upper and lower clip of cutting is truncate, and upper clip is apart from clip bud 0.5～1cm, and clip is level and smooth, and every root cutting has 2～4 full buds;
(5) cuttings is processed and cuttage: the base portion of cuttings was soaked after 8 hours in clear water, with dipping 2min in 0.5% liquor potassic permanganate, insert in the root-growing agent that step (3) prepares and soak 2 hours, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert rear management and transplant: the cuttings being plugged in step (4) is placed in to plastic canopy insulation, moisturizing, wherein encircles temperature of shed and remain on 25 ℃ of left and right, relative moisture reaches more than 80%; After cuttage, every 10～15d, spray 500～800 times of 50% carbendazol wettable powder dilution thimerosals 1 time, prevent from going rotten, remove in time rotten cuttings; After 20 days, cuttings base portion starts to form callus, in the time of 40 days, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root, within 80 days, transplants afterwards, and cuttage survival rate reaches 88%.
Wherein take embodiment 3 as preferred embodiment.
Below with preferred embodiment, disclose the present invention, so it is not intended to limiting the invention, and all employings are equal to replaces or technical scheme that equivalent transformation mode obtains, within all dropping on protection scope of the present invention.
Claims (4)
1. an Acer palmatum hardwood cutting and seedling raising method, is characterized in that, comprises the following steps:
(1) cutting time: be advisable before Acer palmatum rudiment in spring foliation;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into cutting medium by the volume ratio of 1:2～1:3, with 800～1000 times of medicine liquid spray sterilization cutting mediums of 50% carbendazol wettable powder dilution;
(3) preparation root-growing agent: by indolebutyric acid, manganese sulphate, Cobastab
1be mixed in proportion, standby;
(4) prepare cuttings: select Acer palmatum tree crown middle and upper part 1 year raw healthy and strong, lateral bud is full, without the branch of damage by disease and insect, clip length is 8～12cm, diameter is that the branch of 0.3～0.8cm is as cuttings, the upper and lower clip of cutting is truncate, upper clip is apart from clip bud 0.5～1cm, clip is level and smooth, and every root cutting has 2～4 full buds;
(5) cuttings is processed and cuttage: after the base portion of cuttings is soaked respectively in clear water, liquor potassic permanganate, insert in the root-growing agent that step (3) prepares and soak, water permeable after the cutting medium that inserting step (2) configures;
(6) insert after management with transplant: the cuttings of cuttage in step (4) is placed in to plastic canopy insulation, moisturizing, wherein encircles temperature of shed and remain on 25 ℃ of left and right, relative moisture reaches more than 80%; After cuttage, every 10～15d, spray 500～800 times of 50% carbendazol wettable powder dilution thimerosals 1 time, prevent from going rotten, remove in time rotten cuttings; After 20～30 days, cuttings base portion starts to form callus, in the time of 40～60 days, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root, within 70～80 days, transplants afterwards.
2. Acer palmatum hardwood cutting and seedling raising method according to claim 1, is characterized in that, the cutting time described in step (1) is that mid-March in spring is to by the end of March.
3. Acer palmatum hardwood cutting and seedling raising method according to claim 1, is characterized in that, the root-growing agent described in step (3) be indolebutyric acid 200～400mg be dissolved in 20～40ml 95% ethanol in, add manganese sulphate 20～30mg, Cobastab
1after 3.0～5.0mg, add water to 1kg, after mixing, make.
4. Acer palmatum hardwood cutting and seedling raising method according to claim 1, it is characterized in that, it is that the base portion of cuttings was soaked after 6～12 hours in clear water that cuttings described in step (5) is processed, with dipping 1～2min in 0.5% liquor potassic permanganate, insert in the root-growing agent that step (3) prepares and soak 1～2 hour.
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