CN103404326A - North America sweetgum hardwood cuttage seedling raising method - Google Patents

North America sweetgum hardwood cuttage seedling raising method Download PDF

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Publication number
CN103404326A
CN103404326A CN201310202026XA CN201310202026A CN103404326A CN 103404326 A CN103404326 A CN 103404326A CN 201310202026X A CN201310202026X A CN 201310202026XA CN 201310202026 A CN201310202026 A CN 201310202026A CN 103404326 A CN103404326 A CN 103404326A
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China
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cuttings
styraciflua
cuttage
cutting
seedling raising
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CN201310202026XA
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Inventor
窦全琴
黄利斌
史青云
史云光
张荣良
朱艳
蔡鸿宇
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JIANGSU LUYUAN LANDSCAPE CONSTRUCTION CO Ltd
Jiangsu Forestry Academy
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JIANGSU LUYUAN LANDSCAPE CONSTRUCTION CO Ltd
Jiangsu Forestry Academy
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Priority to CN201310202026XA priority Critical patent/CN103404326A/en
Publication of CN103404326A publication Critical patent/CN103404326A/en
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Abstract

The invention discloses a North America sweetgum hardwood cuttage seedling raising method. The method comprises the following steps that (1), cuttings are prepared; (2), cuttage media are prepared; (3), rooting agents are prepared; (4), the cuttings are processed and cuttage is conducted; (5), management after the cuttage and transplantation are carried out. According to the North America sweetgum hardwood cuttage seedling raising method, the survival rate of the cuttage is more than 80%, and the cost of the method is reduced by 40-50% compared with the cost of a North America sweetgum softwood cuttage method; the North America sweetgum hardwood cuttage seedling raising method is easy and convenient to operate and implement, has the advantages of being low in cost, simple in technical requirement, easy to popularize and apply and the like, and has important application and popularization value for North America sweetgum seedling breeding production.

Description

A kind of L. styraciflua hardwood cutting and seedling raising method
Technical field
The present invention relates to a Plants asexual reproduction method, specifically relate to a kind of L. styraciflua hardwood cutting and seedling raising method.
Background technology
L. styraciflua (Acer palmatum) is Aceraceae (Aceraceae) Acer (Acer) deciduous tree or dungarunga, originates in each province, China Yangtze river basin, in Japan, Korea S etc., distribution is also arranged.The L. styraciflua tree performance is graceful, leaf peculiar, and in good day of spring and autumn, color leaf gardenful, be world-renowned foliage tree kind.The L. styraciflua garden-variety is various, and the afforestation application is very extensive.
The vegetative propagation of plant can keep maternal good characteristic, the cultivation in a organized way of the asexual reproduction method of L. styraciflua, grafting and cuttage etc.Wherein, tissue culture technique requires high, complicated operation, production cost costliness; Biennial above L. styraciflua stock need to be first cultivated in propagation by grafiting, then required L. styraciflua kind scion is grafted on stock to the cultivating seedlings phase that therefore need to grow; There is in recent years the scholar to carry out the test of L. styraciflua softwood cutting propagation, the honest summer high temperature of epicormic branch cuttage period, need to possess full exposure spraying system device can implement, and the method has greatly limited seedling manufacturing enterprise and the peasant household of growing seedlings carries out breeding on a large scale of L. styraciflua seedling.The cuttage implementation condition of L. styraciflua is easy, processing ease, and reproductive-cost is lower; By literature search, the domestic report that there is not yet.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of simple to operate, with low cost, L. styraciflua hardwood cutting and seedling raising method that reproductive efficiency is high.
Technical scheme: the present invention is achieved through the following technical solutions:
A kind of L. styraciflua hardwood cutting and seedling raising method, is characterized in that, comprises the following steps:
(1) cutting time: be advisable before L. styraciflua rudiment in spring foliation;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into to cutting medium by the volume ratio of 1: 2~1: 3, dilute 800~1000 times of medicine liquid sprays cutting mediums of sterilizing with 50% carbendazol wettable powder;
(3) preparation root-growing agent: by indolebutyric acid, manganese sulphate, Cobastab 1Be mixed in proportion, standby;
(4) prepare cuttings: select L. styraciflua tree crown middle and upper part gave birth in 1 year healthy and strong, lateral bud is full, without the branch of damage by disease and insect.Clip length is 8~12cm, diameter be the branch of 0.3~0.8cm as cuttings, the upper and lower clip of transplanting a cutting is truncate, upper clip is apart from clip bud 0.5~1cm, clip is level and smooth, every root cutting will have 2~4 full buds;
(5) cuttings is processed and cuttage: the base portion of cuttings is soaked respectively in clear water, liquor potassic permanganate after, insert in the root-growing agent that step (3) prepares and soak, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert after management with transplant: the cuttings of cuttage in step (4) is placed in to plastic canopy insulation, moisturizing, and wherein in shed, temperature remains on 25 ℃ of left and right, and relative moisture reaches more than 80%; After cuttage, spray 500~800 times of 50% carbendazol wettable powder dilution thimerosals 1 time every 10~15d, prevent from going rotten, remove in time the cuttings of rotting; After 20~30 days, the cuttings base portion starts to form callus, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root in the time of 40~60 days, transplants afterwards in 70~80 days.
2, L. styraciflua hardwood cutting and seedling raising method according to claim 1, is characterized in that, the cutting time described in step (1) is that mid-March in spring is to by the end of March;
3, L. styraciflua hardwood cutting and seedling raising method according to claim 1, it is characterized in that, root-growing agent described in step (3) be indolebutyric acid 200~400mg be dissolved in 20~40ml 95% ethanol in, add manganese sulphate 20~30mg, Cobastab 13.0 after~5.0mg, add water to 1kg, make after mixing;
4, L. styraciflua hardwood cutting and seedling raising method according to claim 1, it is characterized in that, it is the base portion of cuttings is soaked to 6~12 hours in clear water after that cuttings described in step (5) is processed, with in 0.5% liquor potassic permanganate, dipping 1~2min, insert in the root-growing agent that step (3) prepares and soaked 1~2 hour.
Beneficial effect: L. styraciflua hardwood cutting and seedling raising method of the present invention, cuttage survival rate reaches more than 80%, than the cost of L. styraciflua epicormic branch cuttage 30%~50%; It is only method for tissue culture cost 20% left and right; With L. styraciflua propagation by grafiting, compare, shorten growing-seedling period 2~3 years.This method is easy to operation, has that cost is low, specification requirement is simple, the advantage such as easily applies, and has important application and promotional value in the production of L. styraciflua sapling multiplication.
Embodiment
Embodiment 1
(1) cutting time: mid-March in spring is to L. styraciflua by the end of March not yet during rudiment;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into to cutting medium by the volume ratio of 1: 2, dilute 800 times of medicine liquid sprays cutting mediums of sterilizing with 50% carbendazol wettable powder;
(3) preparation root-growing agent: indolebutyric acid 200mg is dissolved in 95% the ethanol of 20ml, adds manganese sulphate 20mg, Cobastab 13.0mg after add water to 1kg, after mixing, make root-growing agent, standby;
(4) prepare cuttings: select L. styraciflua tree crown middle and upper part gave birth in 1 year healthy and strong, lateral bud is full, without the branch of damage by disease and insect.Clip length is 8cm, diameter be the branch of 0.3cm as cuttings, the upper and lower clip of transplanting a cutting is truncate, upper clip is apart from clip bud 0.5~1cm, clip is level and smooth, every root cutting has 2~4 full buds.
(5) cuttings is processed and cuttage: the base portion of cuttings is soaked to 6 hours in clear water after, with in 0.5% liquor potassic permanganate, dipping 1min, insert in the root-growing agent that step (3) prepares and soaked 2 hours, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert rear management and transplant: the cuttings be plugged in step (4) is placed in to plastic canopy insulation, moisturizing, and wherein the interior temperature of shed remains on 25 ℃ of left and right, and relative moisture reaches more than 80%; After cuttage, spray 500~800 times of 50% carbendazol wettable powder dilution thimerosals 1 time every 10~15d, prevent from going rotten, remove in time the cuttings of rotting; After 30 days, the cuttings base portion starts to form callus, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root in the time of 60 days, transplants afterwards in 80 days, and cuttage survival rate reaches 83%.
Embodiment 2
(1) cutting time: in extremely not yet rudiment of L. styraciflua by the end of March mid-March in spring;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into to cutting medium by the volume ratio of 1: 2, dilute 800 times of medicine liquid sprays cutting mediums of sterilizing with 50% carbendazol wettable powder;
(3) preparation root-growing agent: indolebutyric acid 400mg is dissolved in 95% the ethanol of 40ml, adds manganese sulphate 30mg, Cobastab 15.0mg after add water to 1kg, after mixing, make root-growing agent, standby;
(4) prepare cuttings: select L. styraciflua tree crown middle and upper part gave birth in 1 year healthy and strong, lateral bud is full, without the branch of damage by disease and insect.Clip length is 10cm, diameter be the branch of 0.5cm as cuttings, the upper and lower clip of transplanting a cutting is truncate, upper clip is apart from clip bud 0.5~1cm, clip is level and smooth, every root cutting has 2~4 full buds;
(5) cuttings is processed and cuttage: the base portion of cuttings is soaked to 12 hours in clear water after, with in 0.5% liquor potassic permanganate, dipping 2min, insert in the root-growing agent that step (3) prepares and soaked 1 hour, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert rear management and transplant: the cuttings be plugged in step (4) is placed in to plastic canopy insulation, moisturizing, and wherein the interior temperature of shed remains on 25 ℃ of left and right, and relative moisture reaches more than 80%; After cuttage, spray 500~800 times of 50% carbendazol wettable powder dilution thimerosals 1 time every 10~15d, prevent from going rotten, remove in time the cuttings of rotting; After 25 days, the cuttings base portion starts to form callus, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root about 50 days, transplants afterwards in 80 days, and cuttage survival rate reaches 86%
Embodiment 3
(1) cutting time: in extremely not yet rudiment of L. styraciflua by the end of March mid-March in spring;
(2) be equipped with cutting medium: vermiculite and peat are mixed and made into to cutting medium by the volume ratio of 1: 3, dilute 1000 times of medicine liquid sprays cutting mediums of sterilizing with 50% carbendazol wettable powder;
(3) preparation root-growing agent: indolebutyric acid 300mg is dissolved in 95% the ethanol of 30ml, adds manganese sulphate 25mg, Cobastab 14.0mg after add water to 1kg, after mixing, make root-growing agent, standby;
(4) prepare cuttings: select L. styraciflua tree crown middle and upper part gave birth in 1 year healthy and strong, lateral bud is full, without the branch of damage by disease and insect.Clip length is 12cm, diameter be the branch of 0.8cm as cuttings, the upper and lower clip of transplanting a cutting is truncate, upper clip is apart from clip bud 0.5~1cm, clip is level and smooth, every root cutting has 2~4 full buds;
(5) cuttings is processed and cuttage: the base portion of cuttings is soaked to 8 hours in clear water after, with in 0.5% liquor potassic permanganate, dipping 2min, insert in the root-growing agent that step (3) prepares and soaked 2 hours, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert rear management and transplant: the cuttings be plugged in step (4) is placed in to plastic canopy insulation, moisturizing, and wherein the interior temperature of shed remains on 25 ℃ of left and right, and relative moisture reaches more than 80%; After cuttage, spray 500~800 times of 50% carbendazol wettable powder dilution thimerosals 1 time every 10~15d, prevent from going rotten, remove in time the cuttings of rotting; After 20 days, the cuttings base portion starts to form callus, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root in the time of 40 days, transplants afterwards in 80 days, and cuttage survival rate reaches 88%.
The embodiment 3 of wherein take is preferred embodiment.
Below with preferred embodiment, disclose the present invention, so it is not intended to limiting the invention, and all employings are equal to replaces or technical scheme that the equivalent transformation mode obtains, within all dropping on protection scope of the present invention.

Claims (4)

1. a L. styraciflua hardwood cutting and seedling raising method, is characterized in that, comprises the following steps:
(1) cuttings is prepared: after L. styraciflua is fallen leaves in the winter time, and clip L. styraciflua branch, the husky Tibetan;
(2) be equipped with cutting medium: perlite and vermiculite are mixed and made into to cutting medium by the volume ratio of 1: 1~1: 2, dilute 800~1000 times of medicine liquid sprays cutting mediums of sterilizing with 50% carbendazol wettable powder;
(3) preparation root-growing agent: by indolebutyric acid, Cobastab 12Be mixed in proportion, standby;
(4) prepare cuttings: at the beginning of next year by the end of February to 3 month, will be cut into through the branch that sand is hidden and be with 2~3 buds, be about the cuttings of 10~13cm, the upper cut straight snips, apart from the about 1~2cm of the 1st bud, lower cut is at 1cm place, joint (the bud back side) below straight snips;
(5) cuttings is processed and cuttage: the base portion of cuttings is soaked respectively in liquor potassic permanganate after, insert in the root-growing agent that step (3) prepares and soak, after the cutting medium that inserting step (2) configures, water permeable;
(6) insert after management with transplant: the cuttings of cuttage in step (4) is placed in to plastic canopy insulation, moisturizing, and wherein in shed, temperature remains on 25 ℃ of left and right, and relative moisture reaches more than 80%; After cuttage, spray 500~800 times of 50% carbendazol wettable powder dilution thimerosals 1 time every 10~15d, prevent from going rotten, remove in time the cuttings of rotting; After 30~40 days, the cuttings base portion starts to form callus, from the callus of cuttings base portion or the skin zone of cuttings base portion, bears adventive root in the time of 50~60 days, transplants afterwards in 80~90 days.
2. L. styraciflua hardwood cutting and seedling raising method according to claim 1, is characterized in that, it is 1 year livings branch of clip L. styraciflua that cuttings described in step (1) is prepared, and it is disposed across to water content is to preserve in 60% the sand that totally wets.
3. L. styraciflua hardwood cutting and seedling raising method according to claim 1, is characterized in that, the root-growing agent described in step (3) be indolebutyric acid 200~400mg be dissolved in 20~40ml 95% ethanol in, add manganese sulphate 20~30mg, Cobastab 11.0 after~3.0mg, add water to 1kg, make after mixing.
4. L. styraciflua hardwood cutting and seedling raising method according to claim 1, it is characterized in that, it is the base portion of cuttings is soaked to 6~12 hours in clear water after that cuttings described in step (5) is processed, with in 0.5% liquor potassic permanganate, dipping 1~2min, insert in the root-growing agent that step (3) prepares and soaked 1~2 hour.
CN201310202026XA 2013-05-28 2013-05-28 North America sweetgum hardwood cuttage seedling raising method Pending CN103404326A (en)

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104067845A (en) * 2014-07-22 2014-10-01 江苏绿苑园林建设有限公司 Edgeworthia chrysantha lindl hard wood cutting seedling method
CN104082020A (en) * 2014-07-22 2014-10-08 江苏绿苑园林建设有限公司 Bridal wreath stiff wood cutting seedling culture method
CN104381058A (en) * 2014-09-30 2015-03-04 长沙果正生态农业科技开发有限公司 Trunk-protection water-retention nursery method after tree transplanting
CN105993790A (en) * 2016-06-08 2016-10-12 江苏农林职业技术学院 White tea hardwood branch cutting seedling method
CN106171880A (en) * 2016-06-28 2016-12-07 广西南宁十分园生态农业科技有限公司 A kind of SHANZHIZI cuttage and seedling culture method
CN106489554A (en) * 2016-12-26 2017-03-15 江苏省林业科学研究院 A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis
CN106613686A (en) * 2016-12-26 2017-05-10 江苏省林业科学研究院 60-year-old aged ulmus parvifolia root segment cutting seedling raising method
CN107173192A (en) * 2017-05-22 2017-09-19 江苏农林职业技术学院 Promote trident maple spray cuttage rooting and improve the medicament processing method of growth potential
CN107173025A (en) * 2017-06-29 2017-09-19 成都苗夫现代苗木科技有限公司 A kind of cypress Rapid Rooting cuttage breeding method
CN107466760A (en) * 2016-06-07 2017-12-15 句容市茅山聚福园林有限公司 A kind of cultural method for promoting L. styraciflua cuttage and quick-propagation to take root
CN110178558A (en) * 2019-06-28 2019-08-30 广西壮族自治区林业科学研究院 A kind of method of sweetgum Water culture nursery
CN114051848A (en) * 2021-11-27 2022-02-18 河北省农林科学院石家庄果树研究所 Birch twig cutting seedling method
CN118435794A (en) * 2024-07-08 2024-08-06 中国林业科学研究院华北林业实验中心 Method for grafting and propagating acer truncatum ancient tree

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102511267A (en) * 2011-12-08 2012-06-27 金陵科技学院 Hardwood cutting seedling raising method for tea plant
CN102668858A (en) * 2012-06-05 2012-09-19 江苏省林业科学研究院 Seedling cultivating method for cutting hard woods of acer palmatum

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102511267A (en) * 2011-12-08 2012-06-27 金陵科技学院 Hardwood cutting seedling raising method for tea plant
CN102668858A (en) * 2012-06-05 2012-09-19 江苏省林业科学研究院 Seedling cultivating method for cutting hard woods of acer palmatum

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
秦桂华等: "彩叶树种――国王枫及繁殖技术", 《中国林副特产》 *
郑科等: "扦插技术研究解析", 《贵州农业科学》 *
高椿翔等: "《速生杨栽培管理技术》", 30 March 2008, 中国林业出版社 *

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082020A (en) * 2014-07-22 2014-10-08 江苏绿苑园林建设有限公司 Bridal wreath stiff wood cutting seedling culture method
CN104067845B (en) * 2014-07-22 2016-06-22 江苏绿苑园林建设有限公司 A kind of Edgeworthia chrysantha Lindl. hardwood cutting and seedling raising method
CN104067845A (en) * 2014-07-22 2014-10-01 江苏绿苑园林建设有限公司 Edgeworthia chrysantha lindl hard wood cutting seedling method
CN104381058A (en) * 2014-09-30 2015-03-04 长沙果正生态农业科技开发有限公司 Trunk-protection water-retention nursery method after tree transplanting
CN107466760A (en) * 2016-06-07 2017-12-15 句容市茅山聚福园林有限公司 A kind of cultural method for promoting L. styraciflua cuttage and quick-propagation to take root
CN105993790A (en) * 2016-06-08 2016-10-12 江苏农林职业技术学院 White tea hardwood branch cutting seedling method
CN105993790B (en) * 2016-06-08 2019-03-22 江苏农林职业技术学院 A kind of white tea hardwood cutting and seedling raising method
CN106171880A (en) * 2016-06-28 2016-12-07 广西南宁十分园生态农业科技有限公司 A kind of SHANZHIZI cuttage and seedling culture method
CN106489554A (en) * 2016-12-26 2017-03-15 江苏省林业科学研究院 A kind of root segment cuttage and seedling culture method of century-old raw old-age group yellow wingceltis
CN106613686A (en) * 2016-12-26 2017-05-10 江苏省林业科学研究院 60-year-old aged ulmus parvifolia root segment cutting seedling raising method
CN107173192A (en) * 2017-05-22 2017-09-19 江苏农林职业技术学院 Promote trident maple spray cuttage rooting and improve the medicament processing method of growth potential
CN107173025A (en) * 2017-06-29 2017-09-19 成都苗夫现代苗木科技有限公司 A kind of cypress Rapid Rooting cuttage breeding method
CN110178558A (en) * 2019-06-28 2019-08-30 广西壮族自治区林业科学研究院 A kind of method of sweetgum Water culture nursery
CN114051848A (en) * 2021-11-27 2022-02-18 河北省农林科学院石家庄果树研究所 Birch twig cutting seedling method
CN118435794A (en) * 2024-07-08 2024-08-06 中国林业科学研究院华北林业实验中心 Method for grafting and propagating acer truncatum ancient tree

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Application publication date: 20131127