CN106613686A - 60-year-old aged ulmus parvifolia root segment cutting seedling raising method - Google Patents
60-year-old aged ulmus parvifolia root segment cutting seedling raising method Download PDFInfo
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- CN106613686A CN106613686A CN201611214434.7A CN201611214434A CN106613686A CN 106613686 A CN106613686 A CN 106613686A CN 201611214434 A CN201611214434 A CN 201611214434A CN 106613686 A CN106613686 A CN 106613686A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Abstract
The invention relates to a 60-year-old aged ulmus parvifolia root segment cutting seedling raising method. The method mainly comprises the following steps of (1) selecting mother roots: excavating seminal roots after leaves fall in October to November, selecting root strips with the thickness of 3 to 5 mm, and connecting the root strips into small segments with the length of 5 to 10 cm, namely the mother roots; (2) storing the mother roots: storing the mother roots in sand, storing the mother roots in sandy soil with the thickness of 5 cm and the relative humidity of 60 percent in the first 10 to 20 days, covering with dry sand with the thickness of 2 to 3 cm, and performing spray irrigation on the upper layer of the sandy soil in 5 to 7 days before cuttage of the mother roots; (3) treating the mother roots: performing ultraviolet irradiation for 2 to 3 minutes at once after taking out the mother roots from the sandy soil and trimming the two ends of the mother roots into horse ear shapes; (4) performing cuttage: flatly burying the trimmed mother roots, with the notches facing downwards, into a substrate, wherein the root burying depth is 0.5 to 1 cm; and (5) performing management after cuttage. The method has important significance in breeding of new variety of ulmus parvifolia, and is high in cuttage survival rate.
Description
Technical field
The present invention relates to the raising technology of Chinese elm, more particularly to the root segment cuttage and seedling culture side of a kind of 60 years raw old-age group's Chinese elms
Method.
Background technology
Chinese elm (Classification system：Ulmus parvifolia Jacq) another name：Leaflet elm.Tree-like grace, attitude is graceful, tree
Skin is mottled, and branches and leaves are fine and closely woven, isolated planting, group planting in garden, or all very suitable with booth pavilion, the configuration of mountain stone.Also may be selected as mining area factory green
Change house seeds.Chinese elm ebon is hard, is available for industrial cut stock；Stem hide fiber is tough, can make rope and staple fibre；It is root, skin, tender
Leaf is used as medicine that swelling and pain relieving, removing toxic substances control heat, and scald is controlled in external application；Leaf soil pesticide, can kill red spider.
The vegetative propagation of Chinese elm can adopt the method such as branch cutting and grafting, but because inventor tries in election effects
Test middle discovery, aged Chinese elm branch cutting or engrafting method, survival rate is low, more defective to be, this vegetative propagation into
Seedling after work, poor growth, vitality is not strong, should not carry out the Clone Selection And Reproduction research of next step.And grind in Chinese elm seed selection
During the germ plasm resource studied carefully is collected, the age of tree of most resource Superior line has been above 60 years, adopts in Clone Selection And Reproduction research
With Cutting root reproduction, because root segment phasic development is young, cytoactive is strong, and not only survival rate is high for cuttage, and the childrenization that raises up seed,
Growth is normal, is highly suitable for Clone Selection And Reproduction.
The content of the invention
In order to solve above-mentioned technical problem, there is provided a kind of cuttage survival rate is high, the aged Chinese elm root segment that juvenile traits are stable
Cuttage and seedling culture method, the present invention provides technical scheme below：
The root segment cuttage and seedling culture method of a kind of 60 years raw old-age group's Chinese elms, comprises the following steps：
(1), female root is chosen：Take kind of a root after falling leaves the 10-11 months, select rugosity to be the root bar of 3-5mm, and root bar is cut into
The segment of 5-10cm length, as female root；
In conventional root insert method, more from the root bar that rugosity is 15-20mm as female root, slightly then it is included root bar diameter
Nutriment and Endogenous Hormone Contents in Vitro it is high, be conducive to the growth of rudiment, but rugosity height means the range of choice of root bar
Diminish, qualified bar quantity tails off, and root bar is more thick is more unfavorable for that it is taken root on the contrary, therefore, the present invention is breakthrough
From less bar of rugosity as female root, on the premise of its rooting rate is ensured, root bar rugosity is made up by subsequent treatment little
The adverse effect that sprout growth is brought.
(2), female root storage：Female root is carried out into husky Tibetan, first 10-20 days, it was 60% to be placed in thickness for 5cm, relative humidity
Store in sandy soil, after be covered with the dry sand of 2-3cm, 5-7 days before female root cuttage, to sandy soil upper strata spraying irrigation is carried out, adjust humidity to
60%-70%；
Conventional female root storage is ensureing to prevent root in the case of not doing sth. in advance rudiment to remain a constant humidity
Section dehydration, differentiation of the invention then that humidity has been carried out to female root of Sha Zangzhong is controlled, and by humidity regulation cuttings can be kept
Vigor, and can to a certain extent improve the content of plant endogenous hormones.
(3), female root is processed：The 2-3 months, take out from sandy soil and carry out immediately after female root ultraviolet irradiation 2-3min, irradiate agent
Measure as 350-450mW/c ㎡, then two ends are trimmed to after horse ear shape the mixed solution for dipping rice washing water and garlic extraneous liquid；
Tests prove that, at short notice, be conducive to improving aged Chinese elm cuttage using the ultraviolet irradiation of optimal dose
Survival rate, female root two ends are trimmed to into horse ear shape is prepared for the plug flat of next step, be conducive to increasing female root with
The contact area and cuttage stepmother root of rice washing water and the contact area of matrix.Rice washing water is dipped before cuttage can promote tangent plane
The healing of wound, accelerates the speed of growth of root system.
(4), cuttage：The female root otch pruned is put down down and is embedded in matrix, bury root depth for 0.5-1cm；Test card
It is bright for aged Chinese elm, it is higher that the plug flat of root segment compares the perpendicular motility rate that is inserted into, and earthing is unsuitable blocked up, and earthing is blocked up to cuttage seeding
Take root it is unfavorable.
(5), manage after cuttage：Cuttage and seedling culture room is kept to be constant temperature and humidity condition 40d, thermostat temperature is 15-16 DEG C, constant humidity
Humidity is 50%-60%.
Conventional cuttage and seedling culture room frequently with 25 DEG C or so of temperature, the selection of seeds and female root for the present invention and
Speech, if still maintaining 25 DEG C of nursery temperature, it will cause rudiment to generate development that is too fast and being unfavorable for root.
Further, in step (1) female root rugosity be 4mm, length be 6cm, the side of female root application present invention of this specification
Survival rate highest during method.
Further, the preparation method of step (3) garlic extraneous liquid is that the garlic taken after crushing is placed in Microwave Extraction
In device, and add ethanol that quality is 15-20 times of garlic quality as extractant, be in 85-95 DEG C, microwave output power
Microwave Extraction is carried out under conditions of 300W, is extracted and filtered after 3-5min, obtain garlic extraneous liquid, add the main of garlic extraneous liquid
Purpose is to prevent from dipping the notch after rice washing water to go bad.
Further, step (4) mesostroma is made up of the following component in terms of mass fraction：Pin Shi soil 50-60 parts, rice chaff
Grey 15-25 parts, green soy bean skin 10-20 parts, coconut palm chaff 5-10 part are dried, the more traditional yellow soil of above-mentioned matrix or yellow soil and silt are mixed
The heavy matrix physical behavior closed more optimizes, and the content of organic matter is high, particularly add it is ground be dried green soy bean skin,
Respiratory metabolism and the metabolism of female root can be effectively facilitated, the Endogenous Hormone Contents in Vitro in female root can be further improved.
Further, step (4) mesostroma is made up of the following component in terms of mass fraction：Pin Shi soil 55 parts, rice chaff ash
20 parts, be dried 15 parts of green soy bean skin, 7.5 parts of coconut palm chaff.
The beneficial effects of the present invention is：
(1), the breakthrough less bar of selection rugosity of the present invention be used as female root, on the premise of its rooting rate is ensured,
The little adverse effect brought to sprout growth of root bar rugosity is made up by subsequent treatment, while so that the range of choice of female root becomes
Greatly.
(2) using the humid control of differentiation when, mother Gen Sha hides, the vigor of female root can be kept.
(3), cuttage father's former wife root can promote the healing of tangent plane wound using ultraviolet and rice washing water process, accelerate root system
The speed of growth.
(4), the cutting medium of improvement can significantly improve the content of endogenous hormones in female root.
(5), the cuttage seeding cultivated using the method for the present invention can be used for the Clone Selection And Reproduction research of Chinese elm.
Specific embodiment
Embodiment 1,
The root segment cuttage and seedling culture method of a kind of 60 years raw old-age group's Chinese elms, comprises the following steps：
(1), female root is chosen：Take kind of a root after falling leaves the 10-11 months, select rugosity to be the root bar of 4mm, and root bar is cut into
The segment of 6cm length, as female root；
(2), female root storage：Female root is carried out husky Tibetan, first 15 days, to be placed in thickness for 5cm, the sandy soil that relative humidity is 60%
Middle storage, after be covered with the dry sand of 3cm, 7 days before female root cuttage, to sandy soil upper strata spraying irrigation is carried out, adjust humidity to 65%；
(3), female root is processed：The 2-3 months, take out from sandy soil and carry out immediately after female root ultraviolet irradiation 2.5min, irradiate agent
Measure as 400mW/c ㎡, then two ends are trimmed to after horse ear shape the mixed solution for dipping rice washing water and garlic extraneous liquid；
(4), cuttage：The female root otch pruned is put down down and is embedded in matrix, bury root depth for 0.8cm.The matrix
It is made up of the following component in terms of mass fraction：55 parts of Pin Shi soil, 20 parts of rice chaff ash, it is dried 15 parts of green soy bean skin, 7.5 parts of coconut palm chaff.
(5), manage after cuttage：Cuttage and seedling culture room is kept to be constant temperature and humidity condition 40d, thermostat temperature is 15 DEG C, and constant humidity is wet
Spend for 55%.
Embodiment 2,
The root segment cuttage and seedling culture method of a kind of 60 years raw old-age group's Chinese elms, comprises the following steps：
(1), female root is chosen：Take kind of a root after falling leaves the 10-11 months, select rugosity to be the root bar of 5mm, and root bar is cut into
The segment of 10cm length, as female root；
(2), female root storage：Female root is carried out husky Tibetan, first 15 days, to be placed in thickness for 5cm, the sandy soil that relative humidity is 60%
Middle storage, after be covered with the dry sand of 3cm, 7 days before female root cuttage, to sandy soil upper strata spraying irrigation is carried out, adjust humidity to 65%；
(3), female root is processed：The 2-3 months, take out from sandy soil and carry out immediately after female root ultraviolet irradiation 2.5min, irradiate agent
Measure as 400mW/c ㎡, then two ends are trimmed to after horse ear shape the mixed solution for dipping rice washing water and garlic extraneous liquid；
(4), cuttage：The female root otch pruned is put down down and is embedded in matrix, bury root depth for 0.8cm.The matrix
It is made up of the following component in terms of mass fraction：55 parts of Pin Shi soil, 20 parts of rice chaff ash, it is dried 15 parts of green soy bean skin, 7.5 parts of coconut palm chaff.
(5), manage after cuttage：Cuttage and seedling culture room is kept to be constant temperature and humidity condition 40d, thermostat temperature is 15 DEG C, and constant humidity is wet
Spend for 55%.
Embodiment 3,
The root segment cuttage and seedling culture method of a kind of 60 years raw old-age group's Chinese elms, comprises the following steps：
(1), female root is chosen：Take kind of a root after falling leaves the 10-11 months, select rugosity to be the root bar of 3mm, and root bar is cut into
The segment of 6cm length, as female root；
(2), female root storage：Female root is carried out husky Tibetan, first 15 days, to be placed in thickness for 5cm, the sandy soil that relative humidity is 60%
Middle storage, after be covered with the dry sand of 2cm, 5 days before female root cuttage, to sandy soil upper strata spraying irrigation is carried out, adjust humidity to 70%；
(3), female root is processed：The 2-3 months, take out from sandy soil and carry out immediately after female root ultraviolet irradiation 2min, exposure dose
For 35mW/c ㎡, then two ends are trimmed to after horse ear shape the mixed solution for dipping rice washing water and garlic extraneous liquid；
(4), cuttage：The female root otch pruned is put down down and is embedded in matrix, bury root depth for 1cm；The matrix by
Following component composition in terms of mass fraction：50 parts of Pin Shi soil, 15 parts of rice chaff ash, it is dried 10 parts of green soy bean skin, 5 parts of coconut palm chaff.
(5), manage after cuttage：Cuttage and seedling culture room is kept to be constant temperature and humidity condition 40d, thermostat temperature is 16 DEG C, and constant humidity is wet
Spend for 60%.
Comparative example 1,
The root segment cuttage and seedling culture method of a kind of 60 years raw old-age group's Chinese elms, comprises the following steps：
(1), female root is chosen：Take kind of a root after falling leaves the 10-11 months, select rugosity to be the root bar of 15mm, and root bar is cut into
The segment of 8cm length, as female root；
(2), female root storage：Female root is carried out into husky Tibetan, remains the humidity of sandy soil 60% or so；
(3), female root is processed：Two ends are trimmed to into horse ear shape；Both ends of the surface dip 2000 times of naphthalene acid solution；
(4), cuttage：The female root otch pruned is put down down and is embedded in matrix, bury root depth for 1cm；The matrix by
Yellow soil, coconut palm chaff, rice chaff ash are with 3:1:1 ratio is mixed；
(5), manage after cuttage：Cuttage and seedling culture room is kept to be constant temperature and humidity condition 40d, thermostat temperature is 15 DEG C, and constant humidity is wet
Spend for 50%.
Comparative example 2,
The root segment cuttage and seedling culture method of a kind of 60 years raw old-age group's Chinese elms, comprises the following steps：
(1), female root is chosen：Take kind of a root after falling leaves the 10-11 months, select rugosity to be the root bar of 4mm, and root bar is cut into
The segment of 6cm length, as female root；
(2), female root storage：Female root is carried out husky Tibetan, first 15 days, to be placed in thickness for 5cm, the sandy soil that relative humidity is 60%
Middle storage, after be covered with the dry sand of 2.5cm, 7 days before female root cuttage, to sandy soil upper strata spraying irrigation is carried out, adjust humidity to 65%；
(3), female root is processed：The 2-3 months, take out from sandy soil and carry out immediately after female root ultraviolet irradiation 3min, exposure dose
For 400mW/c ㎡, then two ends are trimmed to into horse ear shape；
(4), cuttage：The female root otch pruned is erected down and is inserted in matrix, bury root depth for 0.5cm；The matrix
It is made up of the following component in terms of mass fraction：55 parts of Pin Shi soil, 20 parts of rice chaff ash, it is dried 15 parts of green soy bean skin, 7.5 parts of coconut palm chaff.
(5), manage after cuttage：Cuttage and seedling culture room is kept to be constant temperature and humidity condition 40d, thermostat temperature is 25 DEG C, and constant humidity is wet
Spend for 55%.
The method of above-mentioned 3 embodiments and 2 comparative examples is respectively adopted carried out root segment cuttage and seedling culture examination to 60 years raw Chinese elms
Test, test data is as follows：
Analysis upper table understands that embodiment 1 is highly preferred embodiment of the present invention, and the survival rate of Chinese elm cuttage seeding is more than 90%；
Comparative analysis embodiment 1 understands that embodiment 2 take out can cuttage seeding and slightly grow longer using the bigger female root of rugosity with embodiment 2, but
Survival rate is reduced；Comparative analysis embodiment 1 and embodiment 3 understand, matrix affects larger with the survival rate for being compared to cuttage seeding；
Comparative analysis embodiment 1 understands that the improvement of the selection, rice washing water process and matrix of rugosity cooperates can be big with comparative example 1
The big survival rate for improving root segment cuttage, root system development speed and sprout growth speed.
With above-mentioned foundation desirable embodiment of the present invention as enlightenment, by above-mentioned description, relevant staff is complete
Various change and modification can be carried out in the range of without departing from this invention technological thought.This invention it is technical
Scope is not limited to the content on specification, it is necessary to its technical scope is determined according to right.
Claims (5)
1. the root segment cuttage and seedling culture method for giving birth to old-age group's Chinese elm in a kind of 60 years, it is characterised in that comprise the following steps：
(1), female root is chosen：Take kind of a root after falling leaves the 10-11 months, select rugosity to be the root bar of 3-5mm, and root bar is cut into into 5-
The segment of 10cm length, as female root；
(2), female root storage：Female root is carried out husky Tibetan, first 10-20 days, to be placed in thickness for 5cm, the sandy soil that relative humidity is 60%
Middle storage, after be covered with the dry sand of 2-3cm, 5-7 days before female root cuttage, to sandy soil upper strata spraying irrigation is carried out, adjust humidity to 60%-
70%；
(3), female root is processed：The 2-3 months, take out from sandy soil and carry out immediately after female root ultraviolet irradiation 2-3min, exposure dose is
350-450mW/cm2, then two ends are trimmed to after horse ear shape the mixed solution for dipping rice washing water and garlic extraneous liquid；
(4), cuttage：The female root otch pruned is put down down and is embedded in matrix, bury root depth for 0.5-1cm；
(5), manage after cuttage：Cuttage and seedling culture room is kept to be constant temperature and humidity condition 40d, thermostat temperature is 15-16 DEG C, constant humidity humidity
For 50%-60%.
2. the root segment cuttage and seedling culture method for giving birth to old-age group's Chinese elm in a kind of 60 years as claimed in claim 1, it is characterised in that：Step
(1) in female root rugosity be 4mm, length be 6cm.
3. the root segment cuttage and seedling culture method for giving birth to old-age group's Chinese elm in a kind of 60 years as claimed in claim 1, it is characterised in that：Step
(3) preparation method of garlic extraneous liquid described in is that the garlic taken after crushing is placed in Microwave Extraction device, and adds quality to be big
The ethanol of 15-20 times of garlic quality carries out microwave as extractant under conditions of being 300W in 85-95 DEG C, microwave output power
Extract, extract and filtered after 3-5min, obtain garlic extraneous liquid.
4. the root segment cuttage and seedling culture method for giving birth to old-age group's Chinese elm in a kind of 60 years as claimed in claim 1, it is characterised in that step
(4) mesostroma is made up of the following component in terms of mass fraction：Pin Shi soil 50-60 parts, rice chaff ash 15-25 parts, it is dried green soy bean skin
10-20 parts, coconut palm chaff 5-10 part.
5. the root segment cuttage and seedling culture method for giving birth to old-age group's Chinese elm in a kind of 60 years as claimed in claim 4, it is characterised in that step
(4) mesostroma is made up of the following component in terms of mass fraction：55 parts of Pin Shi soil, 20 parts of rice chaff ash, it is dried 15 parts of green soy bean skin, coconut palm
7.5 parts of chaff.
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| CN107306729A (en) * | 2017-09-20 | 2017-11-03 | 上海市园林科学规划研究院 | Asimina triloba buries root propagation method |
| CN108093906A (en) * | 2017-12-28 | 2018-06-01 | 湖北民族学院 | A kind of Cutting root reproduction method of idesia female seedling |
| CN109302930A (en) * | 2018-08-14 | 2019-02-05 | 贵州省兴仁县佳文生态农业开发有限公司 | A kind of tree-like pruning method of Karst Mountains Desertification Area loquat |
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| CN108093906A (en) * | 2017-12-28 | 2018-06-01 | 湖北民族学院 | A kind of Cutting root reproduction method of idesia female seedling |
| CN109302930A (en) * | 2018-08-14 | 2019-02-05 | 贵州省兴仁县佳文生态农业开发有限公司 | A kind of tree-like pruning method of Karst Mountains Desertification Area loquat |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170510 |
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| RJ01 | Rejection of invention patent application after publication |