CN105766512A - Cuttage method for Cinnamomum kanehirai - Google Patents
Cuttage method for Cinnamomum kanehirai Download PDFInfo
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- CN105766512A CN105766512A CN201510799140.4A CN201510799140A CN105766512A CN 105766512 A CN105766512 A CN 105766512A CN 201510799140 A CN201510799140 A CN 201510799140A CN 105766512 A CN105766512 A CN 105766512A
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- Prior art keywords
- cuttage
- cuttings
- cinnamomum kanahirai
- kanahirai hay
- cutting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Abstract
The invention discloses a cuttage method for Cinnamomum kanehirai. The method comprises the steps: arranging cuttage beds in an indoor greenhouse, carrying out deep ploughing and fine turning on original soil of bottoms of seedling beds, then, laying vermiculite and pearlite on the original soil, and carrying out disinfecting so as to obtain treated cuttage beds; picking under-yearling or biennial semi-lignified Cinnamomum kanehirai branches from maternal plants on a sunny day among the first ten days of August, cutting the Cinnamomum kanehirai branches into sections of 8cm to 10cm in a manner of reserving 2 to 3 nodes, removing lower leaves and lateral branches in a manner that each branch reserves one to two leaves and a terminal bud, and taking the brances as cuttings; sequentially soaking biological lower ends of the cuttings into a growth regulator and a fungicide, and then, cutting the cuttings into the cuttage beds by a straight cutting manner; erecting small arched sheds on the cuttage beds, covering the sheds with films, thoroughly watering the seedling beds in one time before sealing, and carrying out regular ventilating after sealing so as to keep the internal humiture of the small arched sheds; and increasing the ventilating time from the middle-later stage of cuttage, after callus formation is achieved, to a rooting stage, and controlling the air humidity to 70% to 80% and controlling the temperature to 25 DEG C to 35 DEG C. According to the method disclosed by the invention, a large amount of roots can grow in two weeks, and the rooting ratio reaches 80% or more.
Description
Technical field
The present invention relates to the cuttage field of plant, particularly relate to the cottage method of a kind of Cinnamomum kanahirai hay.
Background technology
Cinnamomum kanahirai hay Cinnamomum kanehirai Hay is the primary endemic tree in Taiwan Province of China, because limb is sturdy such as ox, so being referred to as " ox " camphor tree.Cinnamomum kanahirai hay is evergreen megaphanerophyte, stem slightly reaches 50cm, the height of tree is up to 30m, these seeds are the most extensive in Taiwan distribution, distribution is all had from the mountain region of height above sea level 200～2000m, but due to past substantial amounts of felling, the most only also have fragmentary distribution in alpine region, be put into Taiwan child care seeds.Cinnamomum kanahirai hay leaf alternate, oblong or avette, tip is tapering, keratin, and petiole, tender leaf are green to kermesinus, full edge or slightly ripple edge.Winter blooming, sympodium panicle, drupe oblate spheroid.Timber is solid valuable, is available for furniture processed, carves, extracts essential oil.Tree crown is thick and strong, and branches and leaves are fresh green, for graceful specimen tree, shade tree.
The most valuable, heartwood inwall or the lodging trunk moist surface on ground at the rotten trunk of Cinnamomum kanahirai hay can grow fungi, produce camphor tree sesame.Because camphor tree sesame is effective in cure and antitumor, antiviral to hepatopathy, antiallergy, reducing blood lipid, the effect such as hypoglycemic, rise price day, market price per kilogram more than 10 ten thousand yuan to the most hundreds thousand of units New Taiwan Currencies, the most also cause Cinnamomum kanahirai hay to be cut down in a large number, natural, ecological is seriously damaged.Therefore, carry out the research of Cinnamomum kanahirai hay seedling-wood breeding technology, actively build Cinnamomum kanahirai hay artificial forest significant to ecological protection, economy, social development.
The Pittosporum cement entomophilous flower of Cinnamomum kanahirai hay, difficulty of being pollinated between elite stand, open in tree crown top more again, subject to windburn, occasionally have seed also just to be eaten evil by bird, beast before prematurity, therefore collect seed extremely difficult.In its natural state, seed drops, and also weak because of sylvan life light, dry branches and fallen leaves is blocked up and is difficult to implantation germinating to seedling.
Therefore the Cinnamomum kanahirai hay cuttage technique developing a kind of Fast-propagation seems the most urgent.
Summary of the invention
It is an object of the invention to provide a kind of simple to operate, Cinnamomum kanahirai hay cottage method of stability and high efficiency.
For achieving the above object, the present invention provides the cottage method of Cinnamomum kanahirai hay, it is characterised in that step is,
Slotting machine prepares: slotting machine is located in indoor booth, original soil deep ploughing bottom seedbed is carefully turned over, then at original soil upper berth vermiculite and perlite, finally processes nursery bed disinfection, the slotting machine after being processed；
Cutting is chosen: select fine day, take robust growth on Cinnamomum kanahirai hay maternal plant, without pest and disease damage give birth to then or biennial semi-lignified Cinnamomum kanahirai hay side shoot branch is as cutting, cutting access time is early August；
Cuttings makes: selected branch length is 8cm-10cm, retains 2-3 joint, and this branch is as cuttings；Removing lower blade and side shoot, each cuttings retains one to two panels leaf and terminal bud；
The hormone root induction of cuttings and cuttage: cuttings biology lower end is soaked in the growth regulator of 0.1-1.1g/L；Soaking sterilization agent again, uses straight cutting method cuttage slotting machine after described process after process；
Managing after cuttage: the added Small plastic shed of slotting machine, overlay film on canopy, the compacting of film surrounding seals, and disposably waters seedbed permeable, periodically ventilate after sealing before sealing, and keeps temperature and humidity in Small plastic shed；Cuttings can be taken out and be observed by the cuttage middle and later periods, then can increase ventilation time to the phase of taking root, reduce air humidity after Callus formation, controls air humidity in 70%-80%, temperature 25 DEG C-35 DEG C.
Further, in described slotting machine preparation process, perlite and vermiculite volume ratio are 1:1.
Further, described cuttings making step is, selected branch length is 8cm-10cm, retains 2-3 joint, and this branch is as cuttings；Cuttings upper cut is flat mouth, and on joint at 1-2cm, lower cut is angle, and at the lower 0.5cm of joint, otch smooths；Removing lower blade and side shoot, each cuttings retains one to two panels leaf and terminal bud, and the blade that will be left behind cuts off 2/3rds, reduces the transpiration of moisture.
Further, in the hormone root induction of described cuttings and cuttage step, growth regulator is IBA, and concentration is 0.7g/L, and soak time is 30min；
Optional, bactericide is processed as using 500 times of liquid soaking disinfection 30s in 25% carbendazol wettable powder；
Optional, during cuttage slotting machine after described process, inserting the soil degree of depth is 3-5cm, spacing in the rows 5cm, line-spacing 7cm.
Further, after described cuttage in management process, every ventilation in 4-5 days 1-2 hour after sealing, keep temperature 25 DEG C-35 DEG C in Small plastic shed, humidity more than 90%；Then reducing air humidity after cuttage middle and later periods i.e. Callus formation to the phase of taking root is 70%-80%.
The present invention is directed to prior art not enough, in terms of management etc., Cinnamomum kanahirai hay cottage propagation is carried out after the collection of cuttage branch, seedbed and the preparation of matrix, cuttage environment, hormone kind, hormone concentration, cuttage system regulation, filter out the optimum condition of Cinnamomum kanahirai hay cottage propagation, provide a kind of simple to operate, method of stability and high efficiency for Cinnamomum kanahirai hay softwood cutting propagation.
Owing to the space of booth is relatively big, temperature is higher, and spraying moisture quickly scatters and disappears in the big regional environment of temperature control green house, the less stable of air humidity, cannot meet the Cinnamomum kanahirai hay strict demand to air humidity, cuttings blade face occurs wilting crimp the most in a large number, affects the survival rate of cuttings.And the present invention uses perlite and vermiculite (volume ratio 1:1) as seedbed, after cuttage, water sufficient water to seedbed, seal with Small plastic shed the most again, utilize nature transpiration effect, only needing a small amount of moisturizing from cuttage to period of taking root, way to manage is extensive simply, low cost.
Cottage method of the present invention, accelerates Cinnamomum kanahirai hay spray and takes root, and promotes that cuttings is grown, not only shortens growing-seedling period, in combination with utilizing the useless branch cut when pruning summer to make cuttings, reduce cost, and beneficially Cinnamomum kanahirai hay cottage propagation industrialization is carried out.
The cottage method of Cinnamomum kanahirai hay of the present invention, takes root in two weeks in a large number, and rooting rate reaches more than 80%, and zonule test even may be up to 100%.
Detailed description of the invention
Embodiments of the invention are described below in detail, and the example of described embodiment is intended to for explaining the present invention, and is not considered as limiting the invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition or carried out according to product description.Agents useful for same or instrument unreceipted production firm person, be can by city available from conventional products.
Embodiment: Cinnamomum kanahirai hay cuttage
Slotting machine prepares:
Experiment is carried out in Fujian Institute of Subtroical Plant's warmhouse booth.Soil deep ploughing in booth is carefully turned over, and carries out soil disinfection with 1% liquor potassic permanganate.And with 1000 times of liquid of 25% carbendazol wettable powder matrix sterilized and add a cover film, open film two days later.Test matrix employing perlite, vermiculite and perlite: three kinds of forms (the results are shown in Table 1) of vermiculite (volume ratio 1:1), and mixed thoroughly, leveling is watered permeable standby.Follow-up test all uses perlite: vermiculite (volume ratio 1:1) form；
Cutting is chosen:
Select fine day, take robust growth on Cinnamomum kanahirai hay maternal plant, without pest and disease damage give birth to then or biennial semi-lignified Cinnamomum kanahirai hay side shoot branch is as cutting, cutting access time is early August；
Cuttings makes:
Selected branch length is 8cm-10cm, retains 2-3 joint, and cuttings upper cut is flat mouth, on joint at 1-2cm；Lower cut is angle, and at the lower 0.5cm of joint, otch smooths；Removing lower blade and side shoot, each cuttings retains one to two panels leaf and terminal bud, and the blade that will be left behind cuts off 2/3rds, reduces the transpiration of moisture；Cuttings 20 is a bundle；
The hormone root induction of cuttings and cuttage:
To soak 30min bottom described cuttings in the growth regulator of 0.6g/L, 0.7g/L, 0.8g/L, 0.9g/L, 1.0g/L, 1.1g/L, solution did not had base portion 3cm, and every 20 cuttings are one group.This step has carried out the contrast test of growth regulator kind and concentration, the results are shown in Table 2.Follow-up test all uses IBA 0.7g/L.Growth regulator is ABT1 root-inducing powder, ABT6 root-inducing powder, ABT7 root-inducing powder, indolebutyric acid (IBA), and wherein ABT1 root-inducing powder, ABT6 root-inducing powder and ABT7 root-inducing powder need when dissolving first to dissolve with alcohol, then dissolve with warm water；Indolebutyric acid (IBA) needs to add a little NaOH hydrotropy when dissolving.
Growth regulator puts into 500 times of liquid soaking disinfection 30s in 25% carbendazol wettable powder after processing under moisture state, prevents fungal infection cuttings.Cuttage uses straight cutting method, matrix is watered permeable, presses lightly on cuttings surrounding matrix after cuttage, be allowed to be fully contacted with cuttings before cuttage, and insertion depth is 3-5cm, spacing in the rows 5cm, line-spacing 7cm.
Manage after cuttage:
Small plastic shed is put up in seedbed, canopy overlying transparent plastic film, and the compacting of film surrounding seals, to keep epidemic disaster in film.Disposably watering seedbed permeable before Mi Fenging, water management uses artificial spraying, there to be the globule to be as the criterion on film.Keep temperature 25 DEG C-35 DEG C in Small plastic shed, humidity more than 90%, when temperature is the highest on film spray cooling.Every ventilation in 4-5 days 1-2 hour.Spray 1000 times of liquid of 25% carbendazol wettable powder, prevent fungal infection cuttings week about.Cultivate two weeks and can go out root, survival rate to more than 80%.
Table 1, different cutting medium are to Cinnamomum kanahirai hay epicormic branch cuttage Rooting effect table
From the data of table 1 it can be seen that cutting medium is vermiculite: during perlite (volume ratio 1:1), its rooting rate is relatively individually for the height of perlite or vermiculite.
Table 2, hormon process affects table to what Cinnamomum kanahirai hay epicormic branch cuttage was taken root
From the data of table 2 it can be seen that growth regulator kind is IBA time, its rooting rate generally higher than growth regulator kind is rooting rate when ABT1, ABT6 or ABT7.When growth regulator is 0.7g/L, its rooting rate is up to 100%.
In summary, in early August, under the airtight subenvironment of Small plastic shed, choose life then or biennial semi-lignified Cinnamomum kanahirai hay side shoot branch makees cuttings as cutting, with the mixture of perlite and vermiculite (volume ratio 1:1) as matrix, use IBA 0.7g/L solution to soak base portion 30min, can increase substantially very much rooting rate, zonule experiment reaches as high as 100%.
Although above it has been shown and described that embodiments of the invention, it is understandable that, above-described embodiment is exemplary, being not considered as limiting the invention, above-described embodiment can be changed in the case of without departing from the principle of the present invention and objective, revises, replace and modification by those of ordinary skill in the art within the scope of the invention.
Claims (5)
1. the cottage method of a Cinnamomum kanahirai hay, it is characterised in that step is,
Slotting machine prepares: slotting machine is located in indoor booth, original soil deep ploughing bottom seedbed is carefully turned over, then at original soil upper berth vermiculite and
Perlite, finally processes nursery bed disinfection, the slotting machine after being processed；
Cutting is chosen: select fine day, takes robust growth on Cinnamomum kanahirai hay maternal plant, giving birth to then or biennial half wood without pest and disease damage
Matter Cinnamomum kanahirai hay side shoot branch is as cutting, and cutting access time is early August；
Cuttings makes: selected branch length is 8cm-10cm, retains 2-3 joint, and this branch is as cuttings；Remove inferior leads
Sheet and side shoot, each cuttings retains one to two panels leaf and terminal bud；
The hormone root induction of cuttings and cuttage: cuttings biology lower end is soaked in the growth regulator of 0.1-1.1g/L；
Straight cutting method cuttage slotting machine after described process is used after soaking sterilization agent processes again；
Managing after cuttage: the added Small plastic shed of slotting machine, overlay film on canopy, the compacting of film surrounding seals, and disposably waters seedbed before sealing
Permeable, seal rear venting, keep temperature and humidity in Small plastic shed；Cuttings can be taken out and be observed by the cuttage middle and later periods, treats callus
Then can increase ventilation time after formation to the phase of taking root, reduce air humidity, control air humidity in 70%-80%, temperature 25 DEG C
-35℃。
2. the cottage method of Cinnamomum kanahirai hay described in claim 1, it is characterised in that in described slotting machine preparation process, perlite and vermiculite
Volume ratio is 1:1.
3. the cottage method of Cinnamomum kanahirai hay described in claim 1, it is characterised in that described cuttings making step is, selected branch length
For 8cm-10cm, retaining 2-3 joint, this branch is as cuttings；Cuttings upper cut is flat mouth, on joint at 1-2cm, under
Otch is angle, and at the lower 0.5cm of joint, otch smooths；Removing lower blade and side shoot, each cuttings retains one to two panels
Leaf and terminal bud, the blade that will be left behind cuts off 2/3rds, reduces the transpiration of moisture.
4. the cottage method of Cinnamomum kanahirai hay described in claim 1, it is characterised in that the hormone root induction of described cuttings and cuttage step
In Zhou, growth regulator is IBA, and concentration is 0.7g/L, and soak time is 30min；
Optional, bactericide is processed as using 500 times of liquid soaking disinfection 30s in 25% carbendazol wettable powder；
Optional, during cuttage slotting machine after described process, inserting the soil degree of depth is 3-5cm, spacing in the rows 5cm, line-spacing 7cm.
5. the cottage method of Cinnamomum kanahirai hay described in claim 1, it is characterised in that after described cuttage in management process, every after sealing
Every ventilation in 4-5 days 1-2 hour, keep temperature 25 DEG C-35 DEG C in Small plastic shed, humidity more than 90%；Cuttage middle and later periods i.e. callus
Then reducing air humidity after organizing the formation of to the phase of taking root is 70%-80%.
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| CN201510799140.4A CN105766512A (en) | 2015-11-19 | 2015-11-19 | Cuttage method for Cinnamomum kanehirai |
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| CN201510799140.4A CN105766512A (en) | 2015-11-19 | 2015-11-19 | Cuttage method for Cinnamomum kanehirai |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613817A (en) * | 2016-10-20 | 2017-05-10 | 何荣福 | Camphor tree cuttage substrate |
| CN106922476A (en) * | 2017-04-26 | 2017-07-07 | 贵州大学 | A kind of cultural method for improving the withered cuttage survival rate of rice |
| CN107135874A (en) * | 2017-05-06 | 2017-09-08 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Cuttage and seedling culture method in a kind of Cinnamomum kanahirai hay room |
| CN107915513A (en) * | 2017-11-07 | 2018-04-17 | 南昌工程学院 | A kind of cultural method for being used for fragrant camphor tree cuttage and quickly emerging |
| CN108834597A (en) * | 2018-08-31 | 2018-11-20 | 洪江市金土地生态农业有限责任公司 | A kind of flood eucalyptus camphor tree cuttage and seedling culture method |
| CN109328690A (en) * | 2018-12-10 | 2019-02-15 | 怀化五零三侗医药科技开发有限公司 | Camphor tree cuttage and seedling culture method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201249328A (en) * | 2011-06-07 | 2012-12-16 | Wei-Chih Chang | Cinnamomum micranthum breeding forestation on low-altitude level ground |
| CN103718961A (en) * | 2013-12-18 | 2014-04-16 | 柳州市天姿园艺有限公司 | Sponge liquid culture medium with high cutting survival rate of antrodia camphorata and application of sponge liquid culture medium |
| CN103733991A (en) * | 2013-12-18 | 2014-04-23 | 柳州市天姿园艺有限公司 | Method for cultivating Antrodia camphorate cutting plants |
-
2015
- 2015-11-19 CN CN201510799140.4A patent/CN105766512A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201249328A (en) * | 2011-06-07 | 2012-12-16 | Wei-Chih Chang | Cinnamomum micranthum breeding forestation on low-altitude level ground |
| CN103718961A (en) * | 2013-12-18 | 2014-04-16 | 柳州市天姿园艺有限公司 | Sponge liquid culture medium with high cutting survival rate of antrodia camphorata and application of sponge liquid culture medium |
| CN103733991A (en) * | 2013-12-18 | 2014-04-23 | 柳州市天姿园艺有限公司 | Method for cultivating Antrodia camphorate cutting plants |
Non-Patent Citations (2)
| Title |
|---|
| 周张德堂: "台湾牛樟无性繁殖技术研究", 《中国优秀硕士学位论文全文数据库》 * |
| 曾群生: "牛樟扦插育苗技术研究", 《福建热作科技》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613817A (en) * | 2016-10-20 | 2017-05-10 | 何荣福 | Camphor tree cuttage substrate |
| CN106922476A (en) * | 2017-04-26 | 2017-07-07 | 贵州大学 | A kind of cultural method for improving the withered cuttage survival rate of rice |
| CN107135874A (en) * | 2017-05-06 | 2017-09-08 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Cuttage and seedling culture method in a kind of Cinnamomum kanahirai hay room |
| CN107915513A (en) * | 2017-11-07 | 2018-04-17 | 南昌工程学院 | A kind of cultural method for being used for fragrant camphor tree cuttage and quickly emerging |
| CN108834597A (en) * | 2018-08-31 | 2018-11-20 | 洪江市金土地生态农业有限责任公司 | A kind of flood eucalyptus camphor tree cuttage and seedling culture method |
| CN109328690A (en) * | 2018-12-10 | 2019-02-15 | 怀化五零三侗医药科技开发有限公司 | Camphor tree cuttage and seedling culture method |
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Application publication date: 20160720 |