CN105493994B - The method of hainan holly leaf seed seedling-raising - Google Patents
The method of hainan holly leaf seed seedling-raising Download PDFInfo
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- CN105493994B CN105493994B CN201510869849.7A CN201510869849A CN105493994B CN 105493994 B CN105493994 B CN 105493994B CN 201510869849 A CN201510869849 A CN 201510869849A CN 105493994 B CN105493994 B CN 105493994B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/05—Treatments involving invertebrates, e.g. worms, flies or maggots
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of method of hainan holly leaf seed seedling-raising, hainan holly leaf seed is collected seed and is preserved at being placed in 4 DEG C after drying to the sowing of Second Year spring.Hainan holly leaf seed is placed at 4 DEG C the present invention Screening Treatment preserved to Second Year spring and sow and to hainan holly leaf seed after collecting seed, and drastically increases the germination rate of hainan holly leaf seed.The present invention spray nutritious liquor appropriate by the way that hainan holly leaf seed to be seeded into the seedbed and seedling stage of three layers of matrix, substantially increases the emergence rate of hainan holly leaf seed.
Description
Technical field
The present invention relates to hainan holly leaf field of planting.It is more particularly related to a kind of side of hainan holly leaf seed seedling-raising
Method.
Background technology
Hainan holly leaf is for the processed processing of the dried leaf of holly plant hainan holly leaf Ilex hainanensis Merr.
Into.Hainan holly leaf clear curative effect, usage history are long, recorded in《Guangxi Chinese medicine standard》(nineteen ninety version), its preparation mountain is green
Only by hainan holly leaf, medicinal material is constituted tea hypertension pill simply, is recorded in Chinese medicine ministry standard (mark numbering:WS3-B-2838-98), produce
Product have been applied obtaining national Chinese medicine protection kind.Hainan holly leaf has clearing heat and detoxicating a, swelling and pain relieving, effect of blood circulation and channel invigorating, mainly
Coughed for the hemiplegia caused by lowering blood pressure and blood fat, norcholesterol and coronary heart disease, cerebrovas-cularaccident, and anemopyretic cold, lung heat
Cough, the illness such as edema of throat, tonsillitis, dysentery.It is anti-swollen that pharmacological research also shows that the triterpene compound in hainan holly leaf has
Knurl, antiatherosclerosis, anti-hepatovirus anti-inflammatory and anti-AIDS isoreactivities.The preparation being made as primary raw material using hainan holly leaf as
Step-down, the conventional Chinese patent drug of lipid-loweringing, have the advantages that larger usage amount, determined curative effect, toxic and side effect be small, product is unique, wide
The area such as west Expanding Market, occupation rate of market is high, there is preferable economic benefit and sustainable development prospect.According to《Xinhua's Compendium of Materia Medica》
Record：" hainan holly leaf, also known as hainan holly leaf, cold in nature, bitter through enter liver, spleen, lung, kidney, calm down liver wind, for liver wind headache and liver
The diseases such as positive rising headache, dizziness ".The leading products Cassia hainan holly leaf of Guangxi Wanshoutang Drug Industry Co., Ltd.'s production, hainan holly leaf drop
The patented products such as moulding capsule are shown one's talent in like product, and product annual sales revenue surpasses 20,000,000 yuan.
The former plant hainan holly leaf of hainan holly leaf is Aquifoliaceae Holly, is distributed mainly on Guangxi, Guangdong, Hainan and cloud
A southern band, is mainly distributed near Daming Shan Mountain on each county mountain more than 600-800m above sea level in Guangxi District, once in golden show shrine
Mountain, Heng County, all ground such as peace, Baise Cenwanglaoshan are it has also been found that have a small amount of distribution, the hainan holly leaf medicinal material of current pension parachute is all from
The ground such as upper woods, Wuming, Binyang, Mashan near Daming Shan Mountain, are wild herb resource, not yet there is cultivation history so far.
Since being produced in batches from patented products such as mountain green tea decompression capsules in 2006, it is more than 100 tons of hainan holly leaf annual requirement (fresh leaf), warp
After collection for many years, wild resource limited at present has faced exhaustion, and usually supply falls short of demand for medicinal material raw material, causes medicinal material valency
Lattice constantly raise up.In order to meet the Production requirement of enterprise, it is necessary to carry out the artificial cultivation of large area coming as medicinal raw material
Source.
At present, wild hainan holly leaf mainly passes through seminal propagation, because percentage of seedgermination is extremely low under natural conditions, it is impossible to meet
The need for large area artificial cultivation, therefore it is badly in need of the method for finding out a set of hainan holly leaf seed seedling-raising.Through retrieving, so far,
The patent in terms of hainan holly leaf seed seedling-raising is there are no both at home and abroad.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantage that at least will be described later.
It is a still further object of the present invention to provide a kind of method of hainan holly leaf seed seedling-raising, by after seed collecting by hainan holly leaf kind
Son is placed at 4 DEG C the Screening Treatment preserved to Second Year spring and sow and to hainan holly leaf seed, drastically increases hainan holly leaf
The germination rate of seed.
A further object of the invention is seedbed and the appropriate seedling stage by the way that hainan holly leaf seed to be seeded into three layers of matrix
Spray nutritious liquor, substantially increase the emergence rate of hainan holly leaf seed.
In order to realize these purposes of the invention and further advantage, there is provided a kind of side of hainan holly leaf seed seedling-raising
Method, hainan holly leaf seed is collected seed and is preserved at being placed in 4 DEG C after drying to the sowing of Second Year spring.
Preferably, the method for described hainan holly leaf seed seedling-raising, annual mid or late October, treats that hainan holly leaf berry reddens
When, collect seed in time, when pericarp keeps moistening, berry is put into water, rub the pericarp of berry with the hands rotten, seed is washed out, and remove
The floater for floating on the surface, the demersal full seed of collection obtains first and treats sowing, is store at 4 DEG C are placed in after drying
Hide.
Preferably, the method for described hainan holly leaf seed seedling-raising, also includes：
Step A, seedbed prepares：Set up seedling cultivation bed, seedbed 1.0-1.2m wide, 6-10cm high, medium of seedling bed are three layers of base
Matter, wherein, bottom matrix is nursery soil, garden mould and river sand by volume 1:1:1 is well mixed, and thickness is 6-10cm, intermediate layer
Matrix is yellow clay, and thickness is 0.4-0.6cm, and overlayer matrix is nursery soil, and thickness is 0.05-0.15cm；
Step B, Seeds preprocess：After hainan holly leaf seed is taken out under the conditions of 4 DEG C, with 1000mg/L potassium permanganate warm water
After solution immersion 2-3h, clear water is washed 2-3 times, then with the gibberellin aqueous solution that concentration is 100-300mg/L, soaks seed
30min；
Step C, seeding and seedling raising：Sowed in March to the April of Second Year, by seed and river sand by volume 1:3 is uniform mixed
Close, be sown in the intermediate layer matrix in the seedbed for preparing in advance, drench sufficient water, and film shed is built above seedbed.
Preferably, the method for described hainan holly leaf seed seedling-raising, also includes：
Step D, seedling management：After planting, the humidity for keeping seedling bed is 50%-70%, treats that long to 2~3 of seedling is true
Ye Hou, every 7 days to foliage-spray nutritious liquor, the formula of the nutrient solution is：Urea 1-1.5g/L, potassium dihydrogen phosphate 1.5-
2.5g/L, boric acid 6.0mg/L, glycine 2.0mg/L and vitamin B6 0.5mg/L。
Preferably, the method for described hainan holly leaf seed seedling-raising, in seedling management, after after seed sprouting, take by
Step take off film hardening method, first open shed two ends film divulge information 2-3 days, then open shed both sides film ventilation 4-5 days,
Finally open whole films.
Preferably, the method for described hainan holly leaf seed seedling-raising, after seed sprouting, alternately 1 concentration was sprayed per 3-5 days
It is the carbendazim of 300mg/L, sprays 2-3 times altogether.
Preferably, the method for described hainan holly leaf seed seedling-raising, also including sieve kind, specially：Sowing is treated by first,
Stored 3-5 days at being placed in 4 DEG C, then taken out, be placed in the sucrose solution that mass fraction is 10%-15%, in 35-38 DEG C of ultrasound
Power is ultrasonication 30-35min under conditions of 350-400W, is stood, and removes suspension seed, and the seed for sinking is taken out,
Microwave 10-15s under the microwave condition that microwave power is 500-550W is placed in, second is obtained and is treated sowing, store at 4 DEG C are placed in after drying
Hide.
Preferably, the method for described hainan holly leaf seed seedling-raising, the preparation method of the nursery soil is as follows：
Step one, by pig manure, chicken manure and peat soil according to mass ratio be 2:1:3 mixing constitute to obtain the first matrix, according still further to
Every cubic metre of first matrix introduces 20-25 bars earthworm and the yellow meal worm of 30-45 bar larval phases cultivates 30-35 days, then every cube
It is the sucrose solution immersion 10-12h of 50%-55% that the first matrix of rice adds 100-150 mass per liter fraction, obtains the second matrix；
Step 2, by 8-10 times that its weight is added in second matrix of deionized water, inoculation microbial inoculum is in 38-40 DEG C
Lower sealed fermenting 8-10 days, filters to get filtrate and filters thing, wherein, the inoculum concentration of the microbial inoculum is second matrix weight
1.5%-2.5%, the microbial inoculum is that bacillus subtilis, actinomyces and phosphate solubilizing bacteria are 1-2 according to mass ratio:2-3:1 composition；
Step 3, the photocatalyst of the 0.8%-1.0% of its weight will be added in thing in the filter, at ultraviolet irradiation is lower
Reason 48h, obtains the 3rd matrix；
Step 4, according to mass ratio it is 1000 by the 3rd matrix and bog moss, bamboo vinegar and mulberry enzyme:250-
300:42-50:The nursery soil is obtained after 22-28 mixing, wherein, the mulberry enzyme is mulberries and white granulated sugar according to mass ratio
It is 3:1 fermentation 20-25 days is obtained.
Preferably, the method for described hainan holly leaf seed seedling-raising, with bamboo vinegar, deionized water after the filtrate is sterilized
It is 10-15 according to mass ratio:1:Liquid manure is obtained after 90 mixing, is applied around seedling in ring after seed sprouting, at interval of each strain in 3-5 days
Miao Huan applies 100mL until stopping ring after long to 2~3 true leaves of seedling applies the liquid manure.
The present invention at least includes following beneficial effect：
1) germination rate of hainan holly leaf seed seedling-raising is extremely low, and the method for the present invention drastically increases hainan holly leaf seed seedling-raising
Germination rate, while by the seeding in nursery bed nursery constituted using three layers of matrix, and timely and appropriately in seedling spraying nutrient solution, promote
Enter taking root, growing for seed, improve emergence rate；
2) present invention, using screening twice, eliminates the seed for being difficult to sprout by hainan holly leaf seed, substantially increases
Germination rate；
3) activity of the nursery soil of the invention by earthworm and yellow meal worm to the first matrix exists pig manure, chicken manure and peat soil
Own metabolism produces wormcast and bloom frass while becoming thoroughly decomposed, and wherein wormcast provides hainan holly leaf Seed Germination and growth institute
The various nutrients for needing, will not cause to burn to seed and seedling, and the ability with absorption and holding nutrients, bloom frass tool
There is activating soil, strengthen the advantages of fertility, nutrient equilibrium, the sugar of high concentration promotes earthworm and yellow meal worm dehydration lethal, as
One of nutriment source of nursery soil, through bacillus subtilis, actinomyces and phosphate solubilizing bacteria fermentation process after, synthesized various B races dimension
Raw element, produces subtilin, polymyxins, nystatin, gramicidins isoreactivity material, and soil pathogenic bacteria and insect pest are suppressed
Effect enhancing, while improve the utilization rate that phosphorus is absorbed by seedling, survives so as to be favorably improved taking root for seedling, greatly improves
Planting percent；Bactericidal effect is obvious under ultraviolet light for photocatalyst, and can decompose bacterium that may be present or fungi release in matrix
Toxin, also with deodorization functions, improve nursery soil applicability and acceptance；Bog moss has powerful storage capacity,
For growth water provides backing after seed seedling, bamboo vinegar has the effect of antibacterial, hestening rooting, germination and growth, and it oozes
Permeability is strong, it is easy to which seed and seedling absorb, and mulberry enzyme has promotion seed or seedling metabolism, and enhancing seedling life is lived
Power, improves the effect of planting percent；To sum up, commercially available nursery soil is replaced to make seedbed using nursery of the invention soil, with reference to this hair
Bright liquid manure is used together, and considerably improves the planting percent of hainan holly leaf seed seedling-raising.
Further advantage of the invention, target and feature embody part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail, to make those skilled in the art's reference say
Bright book word can be implemented according to this.
It should be noted that experimental technique described in following embodiments, unless otherwise specified, is conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1：
A kind of method of hainan holly leaf seed seedling-raising, hainan holly leaf seed is collected seed at being placed in 4 DEG C after drying and is preserved to the Second Year spring
Season is sowed.
Wherein, the method for described hainan holly leaf seed seedling-raising, annual mid or late October, when hainan holly leaf berry reddens, and
When collect seed, when pericarp keeps moistening, berry is put into water, rub the pericarp of berry with the hands rotten, wash out seed, and remove and float over water
Floater on face, the demersal full seed of collection obtains first and treats sowing, is preserved at 4 DEG C are placed in after drying.
Wherein, the method for described hainan holly leaf seed seedling-raising, also includes：
Step A, seedbed prepares：Set up seedling cultivation bed, seedbed 1.0m wide, 6cm high, medium of seedling bed are three layers of matrix, wherein,
Bottom matrix is nursery soil, garden mould and river sand by volume 1:1:1 is well mixed, and thickness is 6cm, and intermediate layer matrix is yellow viscous
Soil, thickness is 0.4cm, and overlayer matrix is nursery soil, and thickness is 0.05cm；
Step B, Seeds preprocess：After hainan holly leaf seed is taken out under the conditions of 4 DEG C, with 1000mg/L potassium permanganate warm water
After solution immersion 2h, clear water is washed 2 times, then with the gibberellin aqueous solution that concentration is 100mg/L, soaks seed 30min；
Step C, seeding and seedling raising：Sowed in March to the April of Second Year, by seed and river sand by volume 1:3 is uniform mixed
Close, be sown in the intermediate layer matrix in the seedbed for preparing in advance, drench sufficient water, and film shed is built above seedbed.
Wherein, the method for described hainan holly leaf seed seedling-raising, also includes：
Step D, seedling management：After planting, the humidity for keeping seedling bed is 50%, after after long to 2~3 true leaves of seedling,
Every 7 days to foliage-spray nutritious liquor, the formula of the nutrient solution is：Urea 1g/L, potassium dihydrogen phosphate 1.5g/L, boric acid
6.0mg/L, glycine 2.0mg/L and vitamin B6 0.5mg/L。
Wherein, the method for described hainan holly leaf seed seedling-raising, in seedling management, after after seed sprouting, takes and progressively takes off
The method of film hardening, first open shed two ends film divulge information 2 days, then open shed both sides film divulge information 4 days, finally open
Whole films.
Wherein, the method for described hainan holly leaf seed seedling-raising, after seed sprouting, every 3 days alternatings spray 1 concentration and are
The carbendazim of 300mg/L, sprays 2 times altogether.
Embodiment 2：
A kind of method of hainan holly leaf seed seedling-raising, hainan holly leaf seed is collected seed at being placed in 4 DEG C after drying and is preserved to the Second Year spring
Season is sowed.
Wherein, the method for described hainan holly leaf seed seedling-raising, annual mid or late October, when hainan holly leaf berry reddens, and
When collect seed, when pericarp keeps moistening, berry is put into water, rub the pericarp of berry with the hands rotten, wash out seed, and remove and float over water
Floater on face, the demersal full seed of collection obtains first and treats sowing, is preserved at 4 DEG C are placed in after drying.
Wherein, the method for described hainan holly leaf seed seedling-raising, also includes：
Step A, seedbed prepares：Set up seedling cultivation bed, seedbed 1.1m wide, 8cm high, medium of seedling bed are three layers of matrix, wherein,
Bottom matrix is nursery soil, garden mould and river sand by volume 1:1:1 is well mixed, and thickness is 8cm, and intermediate layer matrix is yellow viscous
Soil, thickness is 0.5cm, and overlayer matrix is nursery soil, and thickness is 0.1cm；
Step B, Seeds preprocess：After hainan holly leaf seed is taken out under the conditions of 4 DEG C, with 1000mg/L potassium permanganate warm water
After solution immersion 2.5h, clear water is washed 3 times, then with the gibberellin aqueous solution that concentration is 200mg/L, soaks seed 30min；
Step C, seeding and seedling raising：Sowed in March to the April of Second Year, by seed and river sand by volume 1:3 is uniform mixed
Close, be sown in the intermediate layer matrix in the seedbed for preparing in advance, drench sufficient water, and film shed is built above seedbed.
Wherein, the method for described hainan holly leaf seed seedling-raising, also includes：
Step D, seedling management：After planting, the humidity for keeping seedling bed is 60%, after after long to 2~3 true leaves of seedling,
Every 7 days to foliage-spray nutritious liquor, the formula of the nutrient solution is：Urea 1.25g/L, potassium dihydrogen phosphate 2.0g/L, boric acid
6.0mg/L, glycine 2.0mg/L and vitamin B6 0.5mg/L。
Wherein, the method for described hainan holly leaf seed seedling-raising, in seedling management, after after seed sprouting, takes and progressively takes off
The method of film hardening, first open shed two ends film divulge information 3 days, then open shed both sides film divulge information 5 days, finally open
Whole films.
Wherein, the method for described hainan holly leaf seed seedling-raising, after seed sprouting, every 4 days alternatings spray 1 concentration and are
The carbendazim of 300mg/L, sprays 3 times altogether.
Embodiment 3：
A kind of method of hainan holly leaf seed seedling-raising, hainan holly leaf seed is collected seed at being placed in 4 DEG C after drying and is preserved to the Second Year spring
Season is sowed.
Wherein, the method for described hainan holly leaf seed seedling-raising, annual mid or late October, when hainan holly leaf berry reddens, and
When collect seed, when pericarp keeps moistening, berry is put into water, rub the pericarp of berry with the hands rotten, wash out seed, and remove and float over water
Floater on face, the demersal full seed of collection obtains first and treats sowing, is preserved at 4 DEG C are placed in after drying.
Wherein, the method for described hainan holly leaf seed seedling-raising, also includes：
Step A, seedbed prepares：Set up seedling cultivation bed, seedbed 1.2m wide, 10cm high, medium of seedling bed are three layers of matrix, its
In, bottom matrix is nursery soil, garden mould and river sand by volume 1:1:1 is well mixed, and thickness is 10cm, and intermediate layer matrix is yellow
Clay, thickness is 0.6cm, and overlayer matrix is nursery soil, and thickness is 0.15cm；
Step B, Seeds preprocess：After hainan holly leaf seed is taken out under the conditions of 4 DEG C, with 1000mg/L potassium permanganate warm water
After solution immersion 3h, clear water is washed 3 times, then with the gibberellin aqueous solution that concentration is 300mg/L, soaks seed 30min；
Step C, seeding and seedling raising：Sowed in March to the April of Second Year, by seed and river sand by volume 1:3 is uniform mixed
Close, be sown in the intermediate layer matrix in the seedbed for preparing in advance, drench sufficient water, and film shed is built above seedbed.
Wherein, the method for described hainan holly leaf seed seedling-raising, also includes：
Step D, seedling management：After planting, the humidity for keeping seedling bed is 70%, after after long to 2~3 true leaves of seedling,
Every 7 days to foliage-spray nutritious liquor, the formula of the nutrient solution is：Urea 1.5g/L, potassium dihydrogen phosphate 2.5g/L, boric acid
6.0mg/L, glycine 2.0mg/L and vitamin B6 0.5mg/L。
Wherein, the method for described hainan holly leaf seed seedling-raising, in seedling management, after after seed sprouting, takes and progressively takes off
The method of film hardening, first open shed two ends film divulge information 3 days, then open shed both sides film divulge information 5 days, finally open
Whole films.
Wherein, the method for described hainan holly leaf seed seedling-raising, after seed sprouting, every 5 days alternatings spray 1 concentration and are
The carbendazim of 300mg/L, sprays 3 times altogether.
Embodiment 4：
On the basis of embodiment 2, the method for described hainan holly leaf seed seedling-raising, also including sieve kind, specially：By first
Sowing is treated, is stored 3 days at being placed in 4 DEG C, then taken out, be placed in the sucrose solution that mass fraction is 10%, in 35 DEG C of ultrasounds
Power is ultrasonication 30min under conditions of 350W, is stood, and removes suspension seed, and the seed for sinking is taken out, and is placed in microwave
Power is microwave 10s under the microwave condition of 500W, obtains second and treats sowing, and Second Year spring is stored at 4 DEG C are placed in after drying
Sowing, other method for culturing seedlings are same as Example 2.
Embodiment 5：
On the basis of embodiment 2, the method for described hainan holly leaf seed seedling-raising, also including sieve kind, specially：By first
Sowing is treated, is stored 4 days at being placed in 4 DEG C, then taken out, be placed in the sucrose solution that mass fraction is 13%, in 36 DEG C of ultrasounds
Power is ultrasonication 33min under conditions of 375W, is stood, and removes suspension seed, and the seed for sinking is taken out, and is placed in microwave
Power is microwave 13s under the microwave condition of 530W, obtains second and treats sowing, and Second Year spring is stored at 4 DEG C are placed in after drying
Sowing, other method for culturing seedlings are same as Example 2.
Embodiment 6：
On the basis of embodiment 2, the method for described hainan holly leaf seed seedling-raising, also including sieve kind, specially：By first
Sowing is treated, is stored 5 days at being placed in 4 DEG C, then taken out, be placed in the sucrose solution that mass fraction is 15%, in 38 DEG C of ultrasounds
Power is ultrasonication 35min under conditions of 400W, is stood, and removes suspension seed, and the seed for sinking is taken out, and is placed in microwave
Power is microwave 15s under the microwave condition of 550W, obtains second and treats sowing, and Second Year spring is stored at 4 DEG C are placed in after drying
Sowing, other method for culturing seedlings are same as Example 2.
Embodiment 7：
On the basis of embodiment 2, the method for described hainan holly leaf seed seedling-raising, the preparation method of the nursery soil is such as
Under：
Step one, by pig manure, chicken manure and peat soil according to mass ratio be 2:1:3 mixing constitute to obtain the first matrix, according still further to
Every cubic metre of first matrix introduces 20 earthworms and 30 yellow meal worms of larval phase cultivate 30 days, then every cubic metre of first matrix
Add the sucrose solution that 100 mass per liter fractions are 50% to soak 10h, obtain the second matrix；
Step 2, by 8 times that its weight is added in second matrix of deionized water, inoculation microbial inoculum is in closed at 38 DEG C
Fermentation 8 days, filters to get filtrate and filters thing, wherein, the inoculum concentration of the microbial inoculum is the 1.5% of second matrix weight, institute
It is 1 according to mass ratio that microbial inoculum is stated for bacillus subtilis, actinomyces and phosphate solubilizing bacteria:2:1 composition；
Step 3, by its weight is added in thing in the filter 0.8% photocatalyst, in the lower treatment 48h of ultraviolet irradiation,
Obtain the 3rd matrix；
Step 4, according to mass ratio it is 1000 by the 3rd matrix and bog moss, bamboo vinegar and mulberry enzyme:250:
42:The nursery soil is obtained after 22 mixing, wherein, the mulberry enzyme is that mulberries and white granulated sugar are 3 according to mass ratio:1 fermentation
It is obtained within 20 days.
Wherein, the method for described hainan holly leaf seed seedling-raising, by the filtrate sterilize after with bamboo vinegar, deionized water according to
Mass ratio is 10:1:Liquid manure is obtained after 90 mixing, is applied around seedling in ring after seed sprouting, applied at interval of 3 days each young plant rings
100mL applies the liquid manure until stopping ring after long to 2~3 true leaves of seedling.
Embodiment 8：
On the basis of embodiment 2, the method for described hainan holly leaf seed seedling-raising, the preparation method of the nursery soil is such as
Under：
Step one, by pig manure, chicken manure and peat soil according to mass ratio be 2:1:3 mixing constitute to obtain the first matrix, according still further to
Every cubic metre of first matrix introduces 23 earthworms and 37 yellow meal worms of larval phase cultivate 33 days, then every cubic metre of first matrix
Add the sucrose solution that 125 mass per liter fractions are 52% to soak 11h, obtain the second matrix；
Step 2, by 9 times that its weight is added in second matrix of deionized water, inoculation microbial inoculum is in closed at 39 DEG C
Fermentation 9 days, filters to get filtrate and filters thing, wherein, the inoculum concentration of the microbial inoculum is the 2.0% of second matrix weight, institute
It is 2 according to mass ratio that microbial inoculum is stated for bacillus subtilis, actinomyces and phosphate solubilizing bacteria:2:1 composition；
Step 3, by its weight is added in thing in the filter 0.9% photocatalyst, in the lower treatment 48h of ultraviolet irradiation,
Obtain the 3rd matrix；
Step 4, according to mass ratio it is 1000 by the 3rd matrix and bog moss, bamboo vinegar and mulberry enzyme:280:
46:The nursery soil is obtained after 25 mixing, wherein, the mulberry enzyme is that mulberries and white granulated sugar are 3 according to mass ratio:1 fermentation
It is obtained within 23 days.
Wherein, the method for described hainan holly leaf seed seedling-raising, by the filtrate sterilize after with bamboo vinegar, deionized water according to
Mass ratio is 13:1:Liquid manure is obtained after 90 mixing, is applied around seedling in ring after seed sprouting, applied at interval of 4 days each young plant rings
100mL applies the liquid manure until stopping ring after long to 2~3 true leaves of seedling.
Embodiment 9：
On the basis of embodiment 2, the method for described hainan holly leaf seed seedling-raising, the preparation method of the nursery soil is such as
Under：
Step one, by pig manure, chicken manure and peat soil according to mass ratio be 2:1:3 mixing constitute to obtain the first matrix, according still further to
Every cubic metre of first matrix introduces 25 earthworms and 45 yellow meal worms of larval phase cultivate 35 days, then every cubic metre of first matrix
Add the sucrose solution that 150 mass per liter fractions are 55% to soak 12h, obtain the second matrix；
Step 2, by 10 times that its weight is added in second matrix of deionized water, inoculation microbial inoculum is in close at 40 DEG C
Fermentation 10 days is closed, thing is filtered to get filtrate and filter, wherein, the inoculum concentration of the microbial inoculum is the 2.5% of second matrix weight,
The microbial inoculum is that bacillus subtilis, actinomyces and phosphate solubilizing bacteria are 2 according to mass ratio:3:1 composition；
Step 3, by its weight is added in thing in the filter 1.0% photocatalyst, in the lower treatment 48h of ultraviolet irradiation,
Obtain the 3rd matrix；
Step 4, according to mass ratio it is 1000 by the 3rd matrix and bog moss, bamboo vinegar and mulberry enzyme:300:
50:The nursery soil is obtained after 28 mixing, wherein, the mulberry enzyme is that mulberries and white granulated sugar are 3 according to mass ratio:1 fermentation
It is obtained within 25 days.
Wherein, the method for described hainan holly leaf seed seedling-raising, by the filtrate sterilize after with bamboo vinegar, deionized water according to
Mass ratio is 15:1:Liquid manure is obtained after 90 mixing, is applied around seedling in ring after seed sprouting, applied at interval of 5 days each young plant rings
100mL applies the liquid manure until stopping ring after long to 2~3 true leaves of seedling.
Embodiment 10：
On the basis of embodiment 9, the method for described hainan holly leaf seed seedling-raising, also including sieve kind, specially：By first
Sowing is treated, is stored 5 days at being placed in 4 DEG C, then taken out, be placed in the sucrose solution that mass fraction is 15%, in 38 DEG C of ultrasounds
Power is ultrasonication 35min under conditions of 400W, is stood, and removes suspension seed, and the seed for sinking is taken out, and is placed in microwave
Power is microwave 15s under the microwave condition of 550W, obtains second and treats sowing, and Second Year spring is stored at 4 DEG C are placed in after drying
Sowing, other method for culturing seedlings are same as Example 6.
Comparative example 1：
On the basis of embodiment 2, by annual mid or late October, when hainan holly leaf berry reddens, show that seed is ripe,
Should collect seed in time.When pericarp keeps moistening, berry is put into water, rubs the pericarp of berry with the hands rotten, wash out seed, and remove floating
Pericarp and floater on the water surface, demersal full seed are collected and are placed on interior and are dried.By what is dried
Seed is sowed.Refrigerated at seed is not placed at into 4 DEG C as different from Example 2, directly sowing, with adopting with broadcasting, other
Method for culturing seedlings it is same as Example 2.
Comparative example 2：
On the basis of embodiment 2, nursery soil is carried out into nursery, other method for culturing seedlings instead of three layers of matrix as seedbed
It is same as Example 2.
Comparative example 3：
The nutrient solution of artificial preparation is not used to spray blade face, other nursery on the basis of embodiment 2, after seed sprouting
Method is same as Example 2.
In order to further illustrate the beneficial effect of the method for hainan holly leaf seed seedling-raising of the invention, present inventor's pin
To the embodiment and comparative example enumerated in table 1 to specifications in implementation method carry out the nursery of hainan holly leaf seed, each
100 hainan holly leaf seeds of implementation method, draw data below, are shown in Table 1.
Influence of the different embodiments of table 1 to hainan holly leaf seed germination rate and emergence rate
| Implementation method | Germination rate | Emergence rate |
| Embodiment 2 | 90% | 80% |
| Embodiment 6 | 98% | 88% |
| Embodiment 9 | 92% | 94% |
| Embodiment 10 | 99% | 96% |
| Comparative example 1 | 20% | 70% |
| Comparative example 2 | 85% | 50% |
| Comparative example 3 | 90% | 55% |
Applicant of the present invention has done a series of storage experiments also directed to seed, specially：Annual mid or late October, treat mountain
When green tea berry reddens, collect seed in time, when pericarp keeps moistening, berry is put into water, rub the pericarp of berry with the hands rotten, wash out
Seed, and remove the floater for floating on the surface, the demersal full seed of collection obtains first and treats sowing, after drying
Be placed in batches 0 DEG C, 4 DEG C, 8 DEG C, preserve at 12 DEG C, and using the method breeding of embodiment 2, draw the germination rate of seed and emerge
Rate is shown in Table 2 respectively.
Influence of the different reserve temperatures of table 2 to hainan holly leaf seed germination rate and emergence rate
| Reserve temperature/DEG C | Germination rate | Emergence rate |
| 0 | 78% | 68% |
| 4 | 90% | 80% |
| 8 | 63% | 72% |
| 12 | 37% | 65% |
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in specification and implementation method
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the embodiment with description.
Claims (5)
1. a kind of method of hainan holly leaf seed seedling-raising, it is characterised in that hainan holly leaf seed collect seed preserved at being placed in 4 DEG C after drying to
Second Year spring is sowed, and specific method is：Annual mid or late October, when hainan holly leaf berry reddens, collect seed in time, protected in pericarp
When holding moistening, berry is put into water, rubs the pericarp of berry with the hands rotten, wash out seed, and remove the not full kind for floating on the surface
Son, the demersal full seed of collection obtains first and treats sowing, first is placed at 4 DEG C after sowing dries and stores 3-5
My god, then take out, it is placed in the sucrose solution that mass fraction is 10%-15%, in 35-38 DEG C of ultrasonic power for 350-400W
Under the conditions of ultrasonication 30-35min, stand, remove suspension seed, by sink seed take out, being placed in microwave power is
Microwave 10-15s under the microwave condition of 500-550W, obtains second and treats sowing, is preserved at 4 DEG C are placed in after drying；
Specifically nursery step is：
Step A, seedbed prepares：Set up seedling cultivation bed, seedbed 1.0-1.2m wide, 6-10cm high, medium of seedling bed are three layers of matrix, its
In, bottom matrix is nursery soil, garden mould and river sand by volume 1:1:1 is well mixed, and thickness is 6-10cm, and intermediate layer matrix is
Yellow clay, thickness is 0.4-0.6cm, and overlayer matrix is nursery soil, and thickness is 0.05-0.15cm；
Step B, Seeds preprocess：After above-mentioned seed is taken out under the conditions of 4 DEG C, soaked with 1000mg/L potassium permanganate warm water solution
After bubble 2-3h, clear water is washed 2-3 times, then with the gibberellin aqueous solution that concentration is 100-300mg/L, soaks seed 30min；
Step C, seeding and seedling raising：Sowed in March to the April of Second Year, by seed and river sand by volume 1:3 uniform mixing, spread
Broadcast in the intermediate layer matrix in the seedbed for preparing in advance, drench sufficient water, and film shed is built above seedbed；
The preparation method of nursery soil is as follows wherein described in above-mentioned steps A：
Step one, by pig manure, chicken manure and peat soil according to mass ratio be 2:1:3 mixing constitute to obtain the first matrix, according still further to often standing
The matrix of square rice first introduces 20-25 bars earthworm and the yellow meal worm of 30-45 bar larval phases and cultivates 30-35 days, then every cubic metre the
It is the sucrose solution immersion 10-12h of 50%-55% that one matrix adds 100-150 mass per liter fraction, obtains the second matrix；
Step 2, by 8-10 times that its weight is added in second matrix of deionized water, inoculation microbial inoculum is in close at 38-40 DEG C
Fermentation 8-10 days is closed, thing is filtered to get filtrate and filter, wherein, the inoculum concentration of the microbial inoculum is second matrix weight
1.5%-2.5%, the microbial inoculum is that bacillus subtilis, actinomyces and phosphate solubilizing bacteria are 1-2 according to mass ratio:2-3:1 composition；
Step 3, the photocatalyst of the 0.8%-1.0% of its weight will be added in thing in the filter, in the lower treatment of ultraviolet irradiation
48h, obtains the 3rd matrix；
Step 4, according to mass ratio it is 1000 by the 3rd matrix and bog moss, bamboo vinegar and mulberry enzyme:250-300:
42-50:The nursery soil is obtained after 22-28 mixing, wherein, the mulberry enzyme is that mulberries and white granulated sugar are 3 according to mass ratio:
1 fermentation 20-25 days is obtained.
2. the method for hainan holly leaf seed seedling-raising as claimed in claim 1, it is characterised in that also include：
Step D, seedling management：After planting, the humidity for keeping seedling bed is 50%-70%, after after long to 2~3 true leaves of seedling,
Every 7 days to foliage-spray nutritious liquor, the formula of the nutrient solution is：Urea 1-1.5g/L, potassium dihydrogen phosphate 1.5-2.5g/L,
Boric acid 6.0mg/L, glycine 2.0mg/L and vitamin B6 0.5mg/L。
3. the method for hainan holly leaf seed seedling-raising as claimed in claim 2, it is characterised in that in seedling management, treat that seed goes out
After seedling, takes the method for progressively taking off film hardening, and the film for first opening shed two ends is divulged information 2-3 days, then opens the thin of shed both sides
Film is divulged information 4-5 days, finally opens whole films.
4. the method for hainan holly leaf seed seedling-raising as claimed in claim 3, it is characterised in that after seed sprouting, the alternating per 3-5 days
It is the carbendazim of 300mg/L to spray 1 concentration, is sprayed 2-3 times altogether.
5. the method for hainan holly leaf seed seedling-raising as claimed in claim 4, it is characterised in that with bamboo vinegar after the filtrate is sterilized
Liquid, deionized water are 10-15 according to mass ratio:1:Liquid manure is obtained after 90 mixing, is applied around seedling in ring after seed sprouting, at interval of
3-5 days each young plant rings apply 100mL until stopping ring after long to 2~3 true leaves of seedling applies the liquid manure.
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| CN107006332B (en) * | 2017-06-14 | 2020-11-24 | 会东县天有老君滩种养专业合作社 | Artificial cultivation method of wild eagle tea |
| CN107173163A (en) * | 2017-07-17 | 2017-09-19 | 昆明秀珍种植有限公司 | The breeding method of Laoyin tea |
| CN110933969A (en) * | 2019-11-08 | 2020-03-31 | 广西南亚热带农业科学研究所 | Method for improving germination rate of litsea cubeba seeds |
| CN112293129A (en) * | 2020-10-30 | 2021-02-02 | 西双版纳绿景农业开发有限公司 | Blended Pu 'er tea planting method, blended Pu' er tea and preparation method thereof |
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| CN104335810A (en) * | 2014-11-10 | 2015-02-11 | 贵州省植物园 | Rhizoma bletillae sexual propagation method |
| CN104472134A (en) * | 2014-11-07 | 2015-04-01 | 房县聚农贸易有限责任公司 | Standard planting method of konjak hybrid seeds |
| CN104969737A (en) * | 2014-04-11 | 2015-10-14 | 劳坤伟 | Seedling raising method for dendrobium officinale |
| CN104982217A (en) * | 2015-08-11 | 2015-10-21 | 玉龙县凤翔旺生态养殖有限责任公司 | Paris polyphylla seed propagating method |
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| CN104969737A (en) * | 2014-04-11 | 2015-10-14 | 劳坤伟 | Seedling raising method for dendrobium officinale |
| CN104472134A (en) * | 2014-11-07 | 2015-04-01 | 房县聚农贸易有限责任公司 | Standard planting method of konjak hybrid seeds |
| CN104335810A (en) * | 2014-11-10 | 2015-02-11 | 贵州省植物园 | Rhizoma bletillae sexual propagation method |
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