CN104335810A - Rhizoma bletillae sexual propagation method - Google Patents

Rhizoma bletillae sexual propagation method Download PDF

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Publication number
CN104335810A
CN104335810A CN201410625089.0A CN201410625089A CN104335810A CN 104335810 A CN104335810 A CN 104335810A CN 201410625089 A CN201410625089 A CN 201410625089A CN 104335810 A CN104335810 A CN 104335810A
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days
fruit
sowing
seedbed
seedling
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CN104335810B (en
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韩见宇
董燕
戚燕强
路黔
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Guizhou Zihui Modern Traditional Chinese Medicine Development Co Ltd
GUIZHOU PROVINCE PLANT GARDEN
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Guizhou Zihui Modern Traditional Chinese Medicine Development Co Ltd
GUIZHOU PROVINCE PLANT GARDEN
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention provides a rhizoma bletillae sexual propagation method, which comprises the following steps of (1) seed harvesting and storage; (2) seedling bed preparation; (3) substrate preparation; (4) seeding method and post seeding management; and (5) young seedling culture. The most proper conditions are artificially provided for rhizoma bletillae seed germination and growth, the fast growth of rhizoma bletillae young seedlings is promoted, a great number of seedlings can be planted in a short period, and the capability of producing rhizoma bletillae breeding seedling in a commercialization way is achieved. The rhizoma bletillae sexual propagation method has the characteristics that the time is short, the effect taking is fast, and the cost is low. In addition, the scaled operation can be realized, and the popularization and the application are easy.

Description

A kind of bletilla sexual reproduction method
Technical field
The invention belongs to medicinal plant artificial planting technique field, particularly relate to a kind of bletilla sexual reproduction method.
Background technology
Bletilla is that the orchid family bletilla belongs to herbaceos perennial, is national secondary endangered plant, the another name bletilla striata, purple orchid.Mainly be distributed in the areas such as the Guizhou on the south China Qinling Mountains, Hubei, Southern Shaanxi.General evergreen broad-leaved sylvan life of being born in height above sea level 100-3200 rice, during roadside thick grass or rock stitch more.Its root-like stock is in white, several connected, therefore named bletilla or white root.
Bletilla platymiscium about has six kinds, is distributed in East Asia, to Burma, China is main producing region, have four kinds, be respectively magnificent bletilla, little bletilla, bletilla and chrysanthemum bletilla, and all can be used as medicine.Bletilla is medicinal with dry tuber, there is effect of astringing to arrest bleeding, clearing heat and promoting diuresis, detumescence and promoting granulation, be widely used in the illnesss such as treatment hemoptysis, haematemesis, traumatism and bleeding, sore swollen toxin, pulmonary tuberculosis hemoptysis, ulcer haemorrhage clinically, there are very large commodity development potentiality.It is that orchid is a kind of simultaneously, is widely used as a kind of flowers again, has good ornamental value.
Summary of the invention
The present invention is the condition in order to utilize needed for bletilla seed germination and seedling growth, realizes the object of the merchandized handling of bletilla seedling, thus provides a kind of bletilla sexual reproduction method.
Solve the problems of the technologies described above adopted technical scheme to be made up of following step:
1, seed collection and storage: the mark of seed maturity, fruit colour is turned yellow green or brown by emerald green, in fruit, seed color becomes light yellow from white; When fruit does not split, fruit band carpopodium is together cut, fruit is spread out and puts in the cool, after seed is dry, puts into sealed bag, is placed in 4 DEG C of refrigerators for subsequent use;
2, seedbed prepares: with the seedbed of the wide 0.9m of brick one-tenth, long 10m, high 0.12m on the level land of spaciousness, stay the service aisle of 0.5m between seedbed, and at the bottom of bed, paving layer of plastic film antiseep, repaves the pine needle of thick layer 5cm;
3, matrix composition: by the by volume 1:1:1:1 ratio mixing of mountain soil, peat, leaf mould, perlite, add the abundant decomposed cow dung of said mixture gross weight 5%, repeatedly stir 5 times, add water and matrix is soaked, be deposited on sun-drenched concrete floor, cover with plastic foil, be exposed to the sun more than 15 days.Add 8cm in seedbed after crossing aperture 0.5cm sieve thick, hug flat matrix;
4, type of seeding and broadcast rear management: sow first 2 days, matrix in seed bed fully irrigated with clear water, covers plastic film moisturizing and prevents foreign matter from entering, waits to broadcast; Seed is taken out from fruit, put into the narrow meshed plastic casing as toothpick class, by each fruit 4 square metres amount, seed is evenly sprinkling upon stromal surface, on seedbed, after broadcasting, does the moisturizing of plastics Small plastic shed, then entirety builds the sunshade net sunshading and temperature reducing that light transmittance is 30% above whole seedbed;
5, seedling fosters: within after bletilla planting seed 20 days under the temperature condition of 20-30 DEG C, can sprout first leaflet, the Nitrogen, Phosphorus and Potassium of 0.1% is sprayed once at the cloudy day when growing second leaf after 45 days, Nitrogen, Phosphorus and Potassium content is N:P:K=15:15:15, sprayed once every 10 days later, after planting 90 days seedling remove plastic film in the cloudy day after growing to 5 leaves, can be used as planting seedlings after 30 days pseudobulb diameters when seedling after 0.5cm.
The beneficial effect of employing technique scheme is:
The present invention for providing the optimum of bletilla seed germination and growth by people, is impelled bletilla growth of seedling rapid, can produce a large amount of seedling in a short time, reach the ability of merchandized handling bletilla seedling.Time is short, instant effect, cost are low is characteristic of the present invention, and can realize scale operation, is easy to apply.
Embodiment
A kind of bletilla sexual reproduction method, embodiment repeat under, it is made up of following step:
1, seed collection and storage: the mark of seed maturity, fruit colour is turned yellow green or brown by emerald green, in fruit, seed color becomes light yellow from white; When fruit does not split, fruit band carpopodium is together cut, fruit is spread out and puts in the cool, after seed is dry, puts into sealed bag, is placed in 4 DEG C of refrigerators for subsequent use;
2, seedbed prepares: with the seedbed of the wide 0.9m of brick one-tenth, long 10m, high 0.12m on the level land of spaciousness, stay the service aisle of 0.5m between seedbed, and at the bottom of bed, paving layer of plastic film antiseep, repaves the pine needle of thick layer 5cm;
3, matrix composition: by the by volume 1:1:1:1 ratio mixing of mountain soil, peat, leaf mould, perlite, add the abundant decomposed cow dung of said mixture gross weight 5%, repeatedly stir 5 times, add water and matrix is soaked, be deposited on sun-drenched concrete floor, cover with plastic foil, be exposed to the sun more than 15 days.Add 8cm in seedbed after crossing aperture 0.5cm sieve thick, hug flat matrix;
4, type of seeding and broadcast rear management: sow first 2 days, matrix in seed bed fully irrigated with clear water, covers plastic film moisturizing and prevents foreign matter from entering, waits to broadcast; Seed is taken out from fruit, put into the narrow meshed plastic casing as toothpick class, by each fruit 4 square metres amount, seed is evenly sprinkling upon stromal surface, on seedbed, after broadcasting, does the moisturizing of plastics Small plastic shed, then entirety builds the sunshade net sunshading and temperature reducing that light transmittance is 30% above whole seedbed;
5, seedling fosters: within after bletilla planting seed 20 days under the temperature condition of 20-30 DEG C, can sprout first leaflet, the Nitrogen, Phosphorus and Potassium of 0.1% is sprayed once at the cloudy day when growing second leaf after 45 days, Nitrogen, Phosphorus and Potassium content is N:P:K=15:15:15, sprayed once every 10 days later, after planting 90 days seedling remove plastic film in the cloudy day after growing to 5 leaves, can be used as planting seedlings after 30 days pseudobulb diameters when seedling after 0.5cm.

Claims (1)

1.一种白及有性繁殖方法,其特征在于:它由下述步骤组成: 1. A white and sexual reproduction method, characterized in that: it consists of the following steps: 1)种子采收与贮藏:种子成熟的标志,果皮颜色由鲜绿色变黄绿色或棕色,果实内种子颜色从白色变成浅黄色;在果实未裂时将果实带果柄一同剪下,将果实摊开放在阴凉处,待种子干后放入密封袋内,置于4℃冰箱内备用; 1) Seed harvesting and storage: It is a sign of mature seeds, the color of the peel changes from bright green to yellow-green or brown, and the color of the seeds inside the fruit changes from white to light yellow; when the fruit is not cracked, cut off the fruit with the stalk, and put The fruit is spread out and placed in a cool place, and after the seeds are dry, put them into a sealed bag and place them in a refrigerator at 4°C for later use; 2)苗床准备:在宽敞的平地上用砖砌成宽0.9m、长10m、高0.12m的苗床,苗床之间留0.5m的工作通道,床底内铺一层塑料薄膜防渗漏,再铺一层厚5cm的松针即可; 2) Seedbed preparation: build a seedbed with a width of 0.9m, a length of 10m, and a height of 0.12m on a spacious flat ground. Spread a layer of 5cm thick pine needles; 3)基质制备:将山土、泥炭、腐叶土、珍珠岩按体积1:1:1:1比例混合,加入上述混合物总重量5%的充分腐熟牛粪,反复翻动5次,加水使基质润湿,堆放在阳光充足的水泥地上,用塑料膜覆盖,暴晒15天以上; 3) Substrate preparation: Mix mountain soil, peat, leaf humus and perlite in a volume ratio of 1:1:1:1, add fully decomposed cow dung of 5% of the total weight of the above mixture, turn it repeatedly 5 times, add water to make the substrate Wet, stack on the concrete floor with sufficient sunlight, cover with plastic film, and expose to the sun for more than 15 days; 过孔径0.5cm筛后加入苗床内8cm厚,搂平基质; After passing through a sieve with an aperture of 0.5 cm, add it to a thickness of 8 cm in the seedbed, and flatten the substrate; 4)播种方法及播后管理:播种前2天,用清水将播种床内基质充分浇透,盖上塑料薄膜保湿并防止异物进入、待播;将种子从果实中取出,放入如牙签盒类的带小孔的塑料盒内,按每个果实4平方米量将种子均匀撒在基质表面,播后于苗床上作塑料小拱棚保湿,再在全部苗床的上方整体建透光度为30%的遮阳网遮阳降温; 4) Sowing method and post-sowing management: 2 days before sowing, fully pour the inner substrate of the seeding bed with clean water, cover with a plastic film to keep moisture and prevent foreign matter from entering, and wait for sowing; take the seeds out of the fruit and put them in a toothpick box In a plastic box with small holes, spread the seeds evenly on the surface of the substrate according to the amount of 4 square meters for each fruit. After sowing, make a small plastic shed on the seedbed to keep moisture, and then build a light transmittance of 30 on the top of the entire seedbed. % of sunshade net shade cooling; 5)幼苗抚育:白及种子播种后在20-30℃的温度条件下20天可以萌发出第一片小叶,45天后长出第二片叶时在阴天喷施0.1%的三元复合肥一次, 三元复合肥含量为N:P:K=15:15:15,以后每隔10天喷施一次,播种后90天幼苗长到5片叶后于阴天除去塑料薄膜,再过30天当幼苗的假鳞茎直径到0.5cm后可作为种苗定植。 5) Seedling tending: After sowing, the first leaflet can germinate under the temperature of 20-30°C for 20 days, and spray 0.1% ternary compound fertilizer on cloudy days when the second leaf grows after 45 days Once, the content of ternary compound fertilizer is N:P:K=15:15:15, and then spray it every 10 days. After 90 days after sowing, the seedlings grow to 5 leaves and remove the plastic film on a cloudy day. When the diameter of the pseudobulb of the seedling reaches 0.5cm, it can be used as a seedling for field planting.
CN201410625089.0A 2014-11-10 2014-11-10 A kind of Pseudobulbus Bletillae (Rhizoma Bletillae) sexual reproduction method Expired - Fee Related CN104335810B (en)

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105009880A (en) * 2015-06-30 2015-11-04 普洱良宝生物科技有限公司 Method for growing bletilla rhizome seedlings
CN105027886A (en) * 2015-06-25 2015-11-11 洪江市金土地生态农业有限责任公司 Rhizoma bletillae seed direct seeding reproduction method
CN105165360A (en) * 2015-09-29 2015-12-23 江苏农林职业技术学院 Bletilla striata cultivation method
CN105493994A (en) * 2015-12-01 2016-04-20 广西壮族自治区药用植物园 Method for seedling raising of folium ilicis hainanensis seeds
CN105519350A (en) * 2015-12-14 2016-04-27 广西壮族自治区药用植物园 Method for rapidly producing Bletilla striata germchit
CN105754864A (en) * 2015-12-14 2016-07-13 广西壮族自治区药用植物园 Bletilla striata mycorrhizal fungus and application thereof
CN105794476A (en) * 2016-04-08 2016-07-27 石家庄市神州花卉研究所有限公司 One-film double-greenhouse seed seedling culture method for wild alpine roses
CN105815067A (en) * 2016-03-22 2016-08-03 贵州大学 Direct seeding breeding method for bletilla striata seeds
CN106171896A (en) * 2016-07-25 2016-12-07 贵州省蕙安农业科技发展有限公司 A kind of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing mating system
CN106358664A (en) * 2016-08-26 2017-02-01 任鑫 Method for sexually breeding Bletilla striata seedlings on large scale by simulating natural conditions
CN107056434A (en) * 2017-03-28 2017-08-18 云南恩润生物科技发展有限公司 Bletilla striata seeds direct sowing and seedling matrix and preparation method thereof
CN107223444A (en) * 2017-07-18 2017-10-03 四川康养健生物科技有限公司 A kind of method that bletilla seed sowing is bred
CN108124732A (en) * 2017-12-01 2018-06-08 亳州学院 Dabie Mountain wild bletilla striata nutrition sowing media configuration method
CN113519551A (en) * 2021-06-21 2021-10-22 贵州省植物园(贵州省园林科学研究所、贵州省植物研究所) Application of mixed nitrogen in promoting growth of rhizoma bletillae and improving photosynthetic efficiency and polysaccharide accumulation

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CN103404341A (en) * 2013-08-05 2013-11-27 陕西师范大学 Direct seeding propagation method for Bletilla Striata in greenhouses
CN103444394A (en) * 2013-08-15 2013-12-18 李子辉 Method for realizing sexual reproduction and seedling culture of bletilla under imitated ecological condition
CN103609312A (en) * 2013-12-02 2014-03-05 华儒强 Outdoor bletilla striata seed sowing and breeding method

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Publication number Priority date Publication date Assignee Title
JP2005143363A (en) * 2003-11-13 2005-06-09 Gakunan Construction Co Ltd Chaff greening substrate
WO2013062502A1 (en) * 2011-10-28 2013-05-02 Gavrilov Valeriy Aleksandrovych Substrate for growing plants
CN103404341A (en) * 2013-08-05 2013-11-27 陕西师范大学 Direct seeding propagation method for Bletilla Striata in greenhouses
CN103444394A (en) * 2013-08-15 2013-12-18 李子辉 Method for realizing sexual reproduction and seedling culture of bletilla under imitated ecological condition
CN103609312A (en) * 2013-12-02 2014-03-05 华儒强 Outdoor bletilla striata seed sowing and breeding method

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105027886A (en) * 2015-06-25 2015-11-11 洪江市金土地生态农业有限责任公司 Rhizoma bletillae seed direct seeding reproduction method
CN105009880A (en) * 2015-06-30 2015-11-04 普洱良宝生物科技有限公司 Method for growing bletilla rhizome seedlings
CN105165360A (en) * 2015-09-29 2015-12-23 江苏农林职业技术学院 Bletilla striata cultivation method
CN105493994A (en) * 2015-12-01 2016-04-20 广西壮族自治区药用植物园 Method for seedling raising of folium ilicis hainanensis seeds
CN105493994B (en) * 2015-12-01 2017-06-06 广西壮族自治区药用植物园 The method of hainan holly leaf seed seedling-raising
CN105519350B (en) * 2015-12-14 2018-07-03 广西壮族自治区药用植物园 The method of quick production bletilla seedling
CN105519350A (en) * 2015-12-14 2016-04-27 广西壮族自治区药用植物园 Method for rapidly producing Bletilla striata germchit
CN105754864A (en) * 2015-12-14 2016-07-13 广西壮族自治区药用植物园 Bletilla striata mycorrhizal fungus and application thereof
CN105754864B (en) * 2015-12-14 2019-07-23 广西壮族自治区药用植物园 Bletilla mycorrhizal fungi and its application
CN105815067A (en) * 2016-03-22 2016-08-03 贵州大学 Direct seeding breeding method for bletilla striata seeds
CN105794476A (en) * 2016-04-08 2016-07-27 石家庄市神州花卉研究所有限公司 One-film double-greenhouse seed seedling culture method for wild alpine roses
CN106171896A (en) * 2016-07-25 2016-12-07 贵州省蕙安农业科技发展有限公司 A kind of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing mating system
CN106358664A (en) * 2016-08-26 2017-02-01 任鑫 Method for sexually breeding Bletilla striata seedlings on large scale by simulating natural conditions
CN107056434A (en) * 2017-03-28 2017-08-18 云南恩润生物科技发展有限公司 Bletilla striata seeds direct sowing and seedling matrix and preparation method thereof
CN107223444A (en) * 2017-07-18 2017-10-03 四川康养健生物科技有限公司 A kind of method that bletilla seed sowing is bred
CN108124732A (en) * 2017-12-01 2018-06-08 亳州学院 Dabie Mountain wild bletilla striata nutrition sowing media configuration method
CN113519551A (en) * 2021-06-21 2021-10-22 贵州省植物园(贵州省园林科学研究所、贵州省植物研究所) Application of mixed nitrogen in promoting growth of rhizoma bletillae and improving photosynthetic efficiency and polysaccharide accumulation
CN113519551B (en) * 2021-06-21 2022-08-05 贵州省植物园(贵州省园林科学研究所、贵州省植物研究所) Application of mixed nitrogen in promoting growth of rhizoma bletillae and improving photosynthetic efficiency and polysaccharide accumulation

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