CN106613264B - Bletilla striata tissue culture seedling transplanting domestication technology - Google Patents
Bletilla striata tissue culture seedling transplanting domestication technology Download PDFInfo
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- CN106613264B CN106613264B CN201611244428.6A CN201611244428A CN106613264B CN 106613264 B CN106613264 B CN 106613264B CN 201611244428 A CN201611244428 A CN 201611244428A CN 106613264 B CN106613264 B CN 106613264B
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- tissue culture
- sandy soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a bletilla striata tissue culture seedling transplanting domestication technology, which comprises the following steps: s1, preparing sandy soil, wherein the sandy soil is one of yellow sandy soil or black sandy soil; parching sandy soil with a big pot to above 80 deg.C, evaporating water in sandy soil and killing parasite eggs, cooling to room temperature, and brushing sandy soil with a fine sieve for use; s2, spreading prepared sandy soil into a bletilla tissue culture seedling bottle which grows to be about 5cm high, and covering the root of the seedling by the thickness of the sandy soil; s3, placing the seedling bottle in the S2 in a tissue culture room for growing for 15-30d, and keeping the room temperature at 20-30 ℃ and the humidity at about 70%. In the training process, the tissue culture seedlings do not need to be cleaned, so that the roots of the seedlings are not damaged, a large amount of labor, medicines and time are saved, and the complicated steps of domestication of the tissue culture seedlings are simplified.
Description
Technical Field
The invention relates to the technical field of transplanting and domesticating bletilla striata tissue culture seedlings, in particular to a technology for transplanting and domesticating bletilla striata tissue culture seedlings.
Background
The culture medium at the root of the tissue culture seedling needs to be cleaned by clear water before the tissue culture seedling is transplanted in the soil, and the tissue culture seedling is disinfected and sprayed with a rooting agent, and then is put in the soil to be domesticated after the seedling grows out of a new root, so that the labor consumption is large, the time consumption is long, the root of the seedling can be damaged when the seedling is washed, and the survival rate of the seedling is low in the transplanting process, and is about 70 percent at most. Therefore, a bletilla striata tissue culture seedling transplanting domestication technology is provided.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a bletilla striata tissue culture seedling transplanting domestication technology.
The invention provides a bletilla striata tissue culture seedling transplanting domestication technology, which comprises the following steps:
s1, preparing sandy soil, wherein the sandy soil is one of yellow sandy soil or black sandy soil; parching sandy soil with a big pot to above 80 deg.C, evaporating water in sandy soil and killing parasite eggs, cooling to room temperature, and brushing sandy soil with a fine sieve for use;
s2, spreading prepared sandy soil into a bletilla tissue culture seedling bottle which grows to be about 5cm high, and covering the root of the seedling by the thickness of the sandy soil;
s3, placing the seedling bottle in the S2 in a tissue culture room for growing for 15-30d, and keeping the room temperature at 20-30 ℃ and the humidity at about 70%;
s4, allowing the culture medium in the tissue culture bottle to be absorbed by sandy soil and then allowing the culture medium to enter the soil for domestication;
s5, preparing old mountain soil and fermented sheep (cattle, horse, etc.) dung soil, and mixing the old mountain soil and the dung soil according to the ratio of 1: 1 as a substrate, and paving a ground heating wire on the bottom surface of the substrate in winter;
s6, putting the tissue culture seedlings of which the culture medium is absorbed by sand into a substrate in a whole bottle, and domesticating the tissue culture seedlings at room temperature of 20-30 ℃ and humidity of more than 60%;
s7, domesticating for 60-90 days, and transplanting after the wild tissue culture seedlings have the pseudobulbs.
Preferably, the transplanting and domesticating processes of the bletilla striata tissue culture seedlings are all carried out in a greenhouse, two layers of sunshade nets cover the outer side of the greenhouse, one layer of sunshade net can be removed firstly, and the other layer of sunshade net can be removed subsequently along with the advancing of the domesticating time; and the greenhouse is ventilated in time.
Preferably, in S5, the matrix is divided into furrows, and the tissue culture seedlings are all planted on the furrows; in the process of manufacturing the matrix, forest humus soil, weed straw humus soil or a small amount of sawdust can be added in the stirring process of the matrix.
The invention has simple process and convenient operation, the tissue culture seedling is not needed to be cleaned in the training process, so the damage to the root of the seedling can not be caused, and the survival rate in the transplanting and seedling hardening process can reach more than 95 percent because the seedling is directly in transitional contact with the soil by the culture medium, thereby saving a large amount of labor, medicines and time and simplifying the complicated steps of tissue culture seedling domestication.
Detailed Description
The invention is further illustrated by the following examples.
Example 1
A bletilla striata tissue culture seedling transplanting domestication technology comprises the following steps:
s1, preparing sandy soil, wherein the sandy soil is one of yellow sandy soil or black sandy soil; parching sandy soil with a big pot to 80 deg.C, evaporating water in sandy soil and killing parasite eggs, cooling to room temperature, and brushing sandy soil with a fine sieve for use;
s2, spreading prepared sandy soil into a bletilla tissue culture seedling bottle growing to 5cm high, and covering the root of the seedling by the thickness of the sandy soil;
s3, placing the seedling bottle in the S2 in a tissue culture room for growing for 15d, and keeping the room temperature at 22 ℃ and the humidity at 70%;
s4, allowing the culture medium in the tissue culture bottle to be absorbed by sandy soil and then allowing the culture medium to enter the soil for domestication;
s5, preparing old mountain soil and fermented sheep (cattle, horse, etc.) dung soil, and mixing the old mountain soil and the dung soil according to the ratio of 1: 1 as a substrate, and paving a ground heating wire on the bottom surface of the substrate in winter;
s6, putting the whole bottle of the tissue culture seedlings of which the culture medium is absorbed by sand into a substrate, and domesticating the tissue culture seedlings by keeping the room temperature at 20 ℃ and the humidity at 60%;
s7, domesticating for 60 days, and transplanting after the wild tissue culture seedlings all have the pseudobulbs.
Example 2
A bletilla striata tissue culture seedling transplanting domestication technology comprises the following steps:
s1, preparing sandy soil, wherein the sandy soil is one of yellow sandy soil or black sandy soil; parching sandy soil with a big pot to 100 deg.C, evaporating water in sandy soil and killing parasite eggs, cooling to room temperature, and brushing sandy soil with a fine sieve for use;
s2, spreading prepared sandy soil into a bletilla tissue culture seedling bottle growing to 6cm high, and covering the root of the seedling by the thickness of the sandy soil;
s3, placing the seedling bottle in the S2 in a tissue culture room for growing for 20d, and keeping the room temperature at 23 ℃ and the humidity at 73%;
s4, allowing the culture medium in the tissue culture bottle to be absorbed by sandy soil and then allowing the culture medium to enter the soil for domestication;
s5, preparing old mountain soil and fermented sheep (cattle, horse, etc.) dung soil, and mixing the old mountain soil and the dung soil according to the ratio of 1: 1 as a substrate, and paving a ground heating wire on the bottom surface of the substrate in winter;
s6, putting the whole bottle of the tissue culture seedling with the culture medium absorbed by sand into a substrate, and domesticating the tissue culture seedling with the room temperature at 23 ℃ and the humidity at 63%;
s7, domesticating for 70 days, and transplanting after the wild culture seedlings all have the pseudobulb.
Example 3
A bletilla striata tissue culture seedling transplanting domestication technology comprises the following steps:
s1, preparing sandy soil, wherein the sandy soil is one of yellow sandy soil or black sandy soil; parching sandy soil with a big pot to 120 deg.C, evaporating water in sandy soil and killing parasite eggs, cooling to room temperature, and brushing sandy soil with a fine sieve for use;
s2, spreading prepared sandy soil into a bletilla tissue culture seedling bottle growing to be 7cm high, and covering the root of the seedling by the thickness of the sandy soil;
s3, placing the seedling bottle in the S2 in a tissue culture room for growing for 25d, and keeping the room temperature at 250 ℃ and the humidity at 75%;
s4, allowing the culture medium in the tissue culture bottle to be absorbed by sandy soil and then allowing the culture medium to enter the soil for domestication;
s5, preparing old mountain soil and fermented sheep (cattle, horse, etc.) dung soil, and mixing the old mountain soil and the dung soil according to the ratio of 1: 1 as a substrate, and paving a ground heating wire on the bottom surface of the substrate in winter;
s6, putting the whole bottle of the tissue culture seedling of which the culture medium is absorbed by sand into a substrate, and domesticating the tissue culture seedling at the room temperature of 27 ℃ and the humidity of 65%;
s7, domesticating for 80 days, and transplanting after pseudobulbs exist in the culture seedlings of the radix codonopsis.
Example 4
A bletilla striata tissue culture seedling transplanting domestication technology comprises the following steps:
s1, preparing sandy soil, wherein the sandy soil is one of yellow sandy soil or black sandy soil; parching sandy soil with a big pot to 130 deg.C, evaporating water in sandy soil and killing parasite eggs, cooling to room temperature, and brushing sandy soil with a fine sieve for use;
s2, spreading prepared sandy soil into a bletilla tissue culture seedling bottle growing to 8cm high, and covering the root of the seedling by the thickness of the sandy soil;
s3, placing the seedling bottle in the S2 in a tissue culture room for growing for 30d, and keeping the room temperature at 30 ℃ and the humidity at 77%;
s4, allowing the culture medium in the tissue culture bottle to be absorbed by sandy soil and then allowing the culture medium to enter the soil for domestication;
s5, preparing old mountain soil and fermented sheep (cattle, horse, etc.) dung soil, and mixing the old mountain soil and the dung soil according to the ratio of 1: 1 as a substrate, and paving a ground heating wire on the bottom surface of the substrate in winter;
s6, putting the whole bottle of the tissue culture seedling with the culture medium absorbed by sand into a substrate, and domesticating the tissue culture seedling with the room temperature at 30 ℃ and the humidity at 68%;
s7, domesticating for 90d, and transplanting after the wild culture seedlings all have the pseudobulb.
According to the method, the transplanting and domesticating processes of the bletilla striata tissue culture seedlings are all carried out in the greenhouse, two layers of sunshade nets cover the outer side of the greenhouse, one layer of sunshade net can be removed firstly along with the advancing of the domesticating time, and then the other layer of sunshade net can be removed; and the greenhouse is ventilated and ventilated timely; in S5, dividing the matrix into ridges, and planting tissue culture seedlings on the ridges; in the process of manufacturing the matrix, forest humus soil, weed straw humus soil or a small amount of sawdust can be added in the stirring process of the matrix, and nutrients can be provided for the regrowth process of seedlings.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (3)
1. A bletilla striata tissue culture seedling transplanting domestication method is characterized by comprising the following steps:
s1, preparing sandy soil, wherein the sandy soil is one of yellow sandy soil or black sandy soil; parching sandy soil with a big pot to above 80 deg.C, evaporating water in sandy soil and killing parasite eggs, cooling to room temperature, and brushing sandy soil with a fine sieve for use;
s2, spreading prepared sandy soil into a bletilla tissue culture seedling bottle which grows to be about 5cm high, and covering the root of the seedling by the thickness of the sandy soil;
s3, placing the seedling bottle in the S2 in a tissue culture room for growing for 15-30d, and keeping the room temperature at 20-30 ℃ and the humidity at about 70%;
s4, allowing the culture medium in the tissue culture bottle to be absorbed by sandy soil and then allowing the culture medium to enter the soil for domestication;
s5, preparing old mountain soil and fermented sheep or cattle or horse dung soil, and mixing the old mountain soil and the dung soil according to the ratio of 1: 1 as a substrate, and paving a ground heating wire on the bottom surface of the substrate in winter;
s6, putting the tissue culture seedlings of which the culture medium is absorbed by sand into a substrate in a whole bottle, and domesticating the tissue culture seedlings at room temperature of 20-30 ℃ and humidity of more than 60%;
s7, domesticating for 60-90 days, and transplanting after the tissue culture seedlings have the pseudobulbs.
2. The bletilla striata tissue culture seedling transplanting and domesticating method according to claim 1, wherein the bletilla striata tissue culture seedling transplanting and domesticating process is carried out in a greenhouse, two layers of sunshade nets are covered outside the greenhouse, one layer of sunshade net is firstly torn off along with the domesticating time, and then the other layer of sunshade net is removed; and the greenhouse is ventilated and ventilated in time.
3. The bletilla striata tissue culture seedling transplanting domestication method as claimed in claim 1, wherein in S5, the matrix is layered, and the tissue culture seedlings are all planted on the layered bed; in the process of manufacturing the matrix, forest humus soil and weed straw humus soil or a small amount of sawdust are added in the stirring process of the matrix.
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| CN107211676A (en) * | 2017-05-26 | 2017-09-29 | 黄山峰源生物科技有限公司 | The acclimation method of bletilla tissue culture seedlings |
| CN107182788A (en) * | 2017-07-11 | 2017-09-22 | 中国科学院武汉植物园 | The propagation method of bletilla seedling |
| CN111133960A (en) * | 2020-02-20 | 2020-05-12 | 江苏农林职业技术学院 | Transplanting method of clematis foraging tissue culture seedlings |
Citations (4)
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|---|---|---|---|---|
| CN1586140A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Soil hardening off for tissue cultured poplar seedling |
| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN105028215A (en) * | 2015-09-17 | 2015-11-11 | 康美药业（文山）药材种植管理有限公司 | Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586140A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Soil hardening off for tissue cultured poplar seedling |
| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN105028215A (en) * | 2015-09-17 | 2015-11-11 | 康美药业（文山）药材种植管理有限公司 | Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
Non-Patent Citations (1)
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| 生根粉对香蕉组培苗移栽的影响;陈炫等;《作物研究》;20111231;第25卷(第6期);第583-585页 * |
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