CN105028215A - Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata - Google Patents
Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata Download PDFInfo
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Abstract
The invention provides a rooting medium for tissue culture of bletilla striata and a tissue culture method of the bletilla striata. The rooting medium for the tissue culture of the bletilla striata comprises nutrient substances and saw powder, and per liter of the rooting medium contains saw powder 160-180 g. The tissue culture method of the bletilla striata comprises the steps that seeds of the bletilla striata are transferred to the rooting medium after germination and differentiation of the seeds of the bletilla striata are conducted, and rooting culture is conducted. According to the rooting medium for the tissue culture of the bletilla striata and the tissue culture method of the bletilla striata, bletilla striata seedlings cultured through the rooting medium has the advantages of being easy to transplant, high in transplanting survival rate, low in cost, free of seedling cleaning and the like.
Description
Technical field
The present invention relates to group training field, in particular to a kind of for the root media of bletilla striata group training and the tissue culture method of the bletilla striata.
Background technology
The bletilla striata is perennial herb bulbous plant, and plant height 18-60 centimetre, is mainly distributed in China, Japan and Upper Myanmar, and the main florescence is in spring, but different because of various places weather, and evening, Winter Solstice, early summer all may be bloomed.The bletilla striata has medical value and Ornamental value widely.The bletilla striata is mainly used in astringing to arrest bleeding, detumescence and promoting granulation, can potted plant indoor appreciation, also can intersperse in the Hua Tai comparatively covered, flower border or one jiao, garden.
Containing ten hundreds of trickle seeds in the capsule of the bletilla striata, after sterilization, select suitable medium, carry out the axenic germination of bletilla striata seeds, to plant, many employing plate methods nursery in production, namely the mode of " seed germination-differentiation-root induction seedling " carries out large-scale production, after last process of rooting culture, bletilla striata plantlet in vitro can be transplanted by bottle outlet, but traditional root media is agar medium, it is easily attached to bletilla striata shoot root portion, not easily take out from vial, also be inconvenient to clean, easily hurt shoot root, have a strong impact on transplanting survival rate.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of root media for the training of bletilla striata group, and described root media easily transplants bletilla striata seedling, has that transplanting survival rate is high, cost is low, without the need to cleaning the advantages such as nursery.
The second object of the present invention is the tissue culture method providing a kind of bletilla striata, and the bletilla striata seedling that described tissue culture method is turned out has that transplanting survival rate is high, low cost and other advantages.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
For a root media for bletilla striata group training, comprise nutriment and sawdust, and often liter of described root media is containing 160-180g sawdust.
Above-mentioned root media adopts sawdust to instead of traditional coagulating agent-agar powder, and when thus avoiding transplanting, bletilla striata shoot root is adhered to the problem needing cleaning by agar, can not injure shoot root, thus improve the transplanting survival rate of bletilla striata seedling because of cleaning.Simultaneously compared with agar powder, sawdust can be recycled, and cost is lower.In addition, the sawdust of 160-180g/L concentration is suitable for taking root of bletilla striata seedling.
In above-mentioned root media, sawdust can adopt the sawdust of any plant, such as: wax gourd tree sawdust, pine tree sawdust, Chinese fir sawdust and eucalyptus sawdust etc., all sawdusts all have the effect of ventilative water conservation, wherein wax gourd tree and Chinese fir sawdust fibre-bearing and carboritride higher, be applicable to needed for plant growth, it is higher that pine tree and eucalyptus contain terpene substances, there is the effect suppressing plant growth, just can use after needing pile fermentation.
Preferably, described nutriment comprises: the MS medium of original concentration, the 0.1-2.0mg/L basic element of cell division and 0.1-1.0mg/L methyl α-naphthyl acetate.
The bletilla striata seedling stem stalk adopting this nutriment to cultivate is sturdy.For improving the quality of the bletilla striata seedling cultivated further, following nutriment can also be adopted
The modified form MS medium of original concentration, the 0.1-2.0mg/L basic element of cell division and 0.1-1.0mg/L methyl α-naphthyl acetate, the formula of described modified form MS medium is: potassium nitrate 1890-1900mg/L, ammonium nitrate 1650-1660mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, potassium dihydrogen phosphate 165-170mg/L, potassium iodide 0.75-0.83mg/L, boric acid 5-6.2mg/L, four water manganese sulphate 22.3-24mg/L, white vitriol 8.6-10mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.03mg/L, cobalt chloride 0.025-0.03mg/L, ferrous sulfate 27.8-30mg/L, ethylenediamine tetra-acetic acid 24.3-26mg/L, inositol 95-100mg/L, nicotinic acid 0.8-1mg/L, puridoxine hydrochloride 0.8-1mg/L, nicotinic acid thiamine 0.8-1mg/L, glycine 3-4mg/L, pH is 5.8-6.0.
On the basis of above-mentioned nutriment, can following material be added: 60-70g/L bananas juice, 30-40g/L murphy juice, 30-40g/L sucrose, to shorten cultivation cycle, improve the robustness of bletilla striata seedling.Particularly, murphy juice plays provides nutriment and somatotrophic effect.Bananas juice, is beneficial to bletilla striata seedling rooting and strengthens root, improves the survival rate of the bletilla striata in outdoor hardening.Sucrose, provides carbon source, makes bletilla striata seedling robust growth, and reduces the pollution of microorganism to a certain extent.
More preferably, the concentration of the basic element of cell division is 0.1-0.2mg/L, and the concentration of methyl α-naphthyl acetate is preferably 0.8-1.0mg/L, and the basic element of cell division preferably selects 6-BA.
Preferably, the formula of described modified form MS medium is: potassium nitrate 1895-1900mg/L, ammonium nitrate 1650-1655mg/L, calcium chloride dihydrate 440-442mg/L, epsom salt 370-373mg/L, potassium dihydrogen phosphate 165-170mg/L, potassium iodide 0.8-0.83mg/L, boric acid 5.5-6.2mg/L, four water manganese sulphate 22.3-23.6mg/L, white vitriol 8.6-10mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.03mg/L, cobalt chloride 0.028-0.03mg/L, ferrous sulfate 27.8-29mg/L, ethylenediamine tetra-acetic acid 24.3-25mg/L, inositol 98-100mg/L, nicotinic acid 0.8-0.9mg/L, puridoxine hydrochloride 0.8-0.9mg/L, nicotinic acid thiamine 0.8-0.9mg/L, glycine 3-3.5mg/L, pH is 5.8-6.0.
The tissue culture method of the bletilla striata, comprises the following steps:
Making bletilla striata seeds through sprouting and differentiation, transferring in root media mentioned above and carrying out culture of rootage.
Root media used in above-mentioned group of training adopts sawdust to instead of traditional coagulating agent-agar powder, when thus avoiding transplanting, bletilla striata shoot root is adhered to the problem needing cleaning by agar, therefore the bletilla striata seedling that this tissue culture method obtains can not injure shoot root because of cleaning, thus improves transplanting survival rate.
Preferably, the medium that described germination process is used is: the described modified form MS medium of original concentration, 0.5-0.6mg/L methyl α-naphthyl acetate and 4.3-4.5g/L agar powder; Preferably, also comprise 30-40g/L murphy juice, 30-40g/L sucrose.
The quality of effect on bletilla striata seedling sprouted has vital impact, and protocorm induces adventitious buds proliferation to adopt above-mentioned medium to promote, make the root of growth more sturdy, blade is more roomy.In addition, containing starch and other UGFs in murphy juice, the sprouting of bletilla striata seeds and the propagation of protocorm is contributed to.The bletilla striata seeds stage of sprouting can not carry out photosynthesis, and lack autophyting ability, need external source carbon source to provide energy, sucrose molecule can pass in and out plant cell, can provide carbon source, makes seed germination and protocorm increment.Agar powder, high-temperature digestion, air-setting is solid gum, mixes to solidify to be convenient to bletilla striata seeds or protocorm better absorbs nutrient with other nutriments.
Preferably, the medium that described atomization is used is: described modified form MS medium, the 2.0-3.0mg/L basic element of cell division, 0.2-0.3mg/L methyl α-naphthyl acetate and the 4.3-4.5g/L agar powder of original concentration.
Adopt this medium can improve differentiation speed and the differentiation effect of bud.
Preferably, 30-40g/L murphy juice is also comprised in above-mentioned sprouting and differential period, 30-40g/L sucrose.For sprouting and differential period provide sufficient nutrition, raising group training speed, improves the robustness of bletilla striata seedling.
Preferably, described sawdust is the sawdust through liquor natrii hypochloritis's cleaning.
Sodium chlorate solution both can sterilization, can remove again the pollutant in sawdust, for group training provides gnotobasis.The method of concrete cleaning can reference: 10% liquor natrii hypochloritis sawdust being put into dilution 5 times soaks after 1 hour, extruding elimination liquor natrii hypochloritis, then puts into clear water and embathe 1 time, the extruding elimination aqueous solution, for subsequent use.
Compared with prior art, beneficial effect of the present invention is:
(1) improve the root media that the training of bletilla striata group is used, to improve transplanting survival rate, and lower culture medium cost.
(2) improved MS medium, to shorten cultivation cycle, reduces pollution rate, reduces costs, improve survival rate.
(3) bletilla striata group training germination medium used and differential medium is improved, to improve the healthy and strong situation of bletilla striata seedling.
(4) nutriment such as murphy juice and bananas juice is added, to improve the root development situation of bletilla striata seedling.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Fig. 1 is that embodiment 10 is at the bletilla striata seedling of culture of rootage after one month;
Fig. 2 is that control group is at the bletilla striata seedling of culture of rootage after one month;
Fig. 3 is that embodiment 10 is at culture of rootage bletilla striata seedling after two months;
Fig. 4 is that control group is at culture of rootage bletilla striata seedling after two months.
Embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting the scope of the invention.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturer suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
The sawdust that hereafter all embodiments are used all cleans through following methods:
10% liquor natrii hypochloritis sawdust being put into dilution 5 times soaked after 1 hour, extruding elimination liquor natrii hypochloritis, then put into clear water and embathe 1 time, the extruding elimination aqueous solution, for subsequent use.
Embodiment 1
A kind of root media for the training of bletilla striata group:
MS medium, 0.1mg/L6-BA, 1.0mg/L methyl α-naphthyl acetate and 160g/L wax gourd sawdust.
Embodiment 2
A kind of root media for the training of bletilla striata group:
MS medium, 2.0mg/L6-BA, 0.1mg/L methyl α-naphthyl acetate and 180g/L wax gourd sawdust.
Embodiment 3
A kind of root media for the training of bletilla striata group:
MS medium, 0.2mg/L6-BA, 0.8mg/L methyl α-naphthyl acetate and 180g/L wax gourd sawdust.
Embodiment 4
A kind of root media for the training of bletilla striata group:
MS medium, 0.1mg/L6-BA, 1.0mg/L methyl α-naphthyl acetate, 60g/L bananas juice, 30g/L murphy juice, 30g/L sucrose and 160g/L wax gourd sawdust.
Embodiment 5
A kind of root media for the training of bletilla striata group:
MS medium, 0.1mg/L6-BA, 1.0mg/L methyl α-naphthyl acetate, 70g/L bananas juice, 40g/L murphy juice, 40g/L sucrose and 160g/L Chinese fir sawdust.
Embodiment 6
A kind of root media for the training of bletilla striata group:
Modified form MS medium, 0.1mg/L6-BA, 1.0mg/L methyl α-naphthyl acetate, 70g/L bananas juice, 40g/L murphy juice, 40g/L sucrose and 160g/L wax gourd sawdust;
Modified form MS medium: fill a prescription as shown in table 1.
The modified form MS medium of table 1 embodiment 1
| Title | Consumption mg/L |
| Potassium nitrate | 1900 |
| Ammonium nitrate | 1650 |
| Calcium chloride dihydrate | 440 |
| Epsom salt | 370 |
| Potassium dihydrogen phosphate | 170 |
| Potassium iodide | 0.83 |
| Boric acid | 6.2 |
| Four water manganese sulphates | 22.3 |
| White vitriol | 8.6 |
| Sodium molybdate | 0.25 |
| Cupric sulfate pentahydrate | 0.025 |
| Cobalt chloride | 0.025 |
| Ferrous sulfate | 27.8 |
| Ethylenediamine tetra-acetic acid | 24.3 |
| Inositol | 100 |
| Nicotinic acid | 0.8 |
| Puridoxine hydrochloride | 0.8 |
| Nicotinic acid thiamine | 0.8 |
| Glycine | 3 |
| pH | 5.8 |
Embodiment 7
A kind of root media for the training of bletilla striata group:
Modified form MS medium, 0.1mg/L6-BA, 1.0mg/L methyl α-naphthyl acetate, 70g/L bananas juice, 40g/L murphy juice, 40g/L sucrose and 160g/L pine tree sawdust; Modified form MS medium: fill a prescription as shown in table 1.
The modified form MS medium of table 2 embodiment 2
| Title | Consumption mg/L |
| Potassium nitrate | 1890 |
| Ammonium nitrate | 1660 |
| Calcium chloride dihydrate | 445 |
| Epsom salt | 375 |
| Potassium dihydrogen phosphate | 170 |
| Potassium iodide | 0.75 |
| Boric acid | 5 |
| Four water manganese sulphates | 24 |
| White vitriol | 10 |
| Sodium molybdate | 0.2 |
| Cupric sulfate pentahydrate | 0.03 |
| Cobalt chloride | 0.03 |
| Ferrous sulfate | 30 |
| Ethylenediamine tetra-acetic acid | 26 |
| Inositol | 95 |
| Nicotinic acid | 1 |
| Puridoxine hydrochloride | 1 |
| Nicotinic acid thiamine | 1 |
| Glycine | 4 |
| pH | 6.0 |
Embodiment 8
A kind of root media for the training of bletilla striata group:
Modified form MS medium, 0.1mg/L6-BA, 1.0mg/L methyl α-naphthyl acetate, 70g/L bananas juice, 40g/L murphy juice, 40g/L sucrose and 160g/L eucalyptus sawdust; Modified form MS medium: fill a prescription as shown in table 1.
The modified form MS medium of table 3 embodiment 3
| Title | Consumption mg/L |
| Potassium nitrate | 1895 |
| Ammonium nitrate | 1655 |
| Calcium chloride dihydrate | 442 |
| Epsom salt | 373 |
| Potassium dihydrogen phosphate | 165 |
| Potassium iodide | 0.8 |
| Boric acid | 5.5 |
| Four water manganese sulphates | 23.6 |
| White vitriol | 10 |
| Sodium molybdate | 0.2 |
| Cupric sulfate pentahydrate | 0.03 |
| Cobalt chloride | 0.028 |
| Ferrous sulfate | 29 |
| Ethylenediamine tetra-acetic acid | 25 |
| Inositol | 98 |
| Nicotinic acid | 0.9 |
| Puridoxine hydrochloride | 0.9 |
| Nicotinic acid thiamine | 0.9 |
| Glycine | 3.5 |
| pH | 5.9 |
Embodiment 9
The tissue culture method of the bletilla striata:
Preparation seed germination medium: MS medium+NAA0.5mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Preparation differential medium: MS medium+6-BA2.0mg/L+NAA0.2mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Root media is as embodiment 1.
First by seed germination 30 days, and then be transferred to differential medium, cultivate 30 days, finally move to root media and cultivate 60 days, above three process condition of culture are identical: temperature 25 DEG C, intensity of illumination 1500lx, light application time 10h/d.
Embodiment 10
The tissue culture method of the bletilla striata:
Preparation seed germination medium: MS medium+NAA0.5mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Preparation differential medium: MS medium+6-BA2.0mg/L+NAA0.2mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Root media is as embodiment 6.
First by seed germination 30 days, and then be transferred to differential medium, cultivate 30 days, finally move to root media and cultivate 60 days, above three process condition of culture are identical: temperature 25 DEG C, intensity of illumination 1500lx, light application time 10h/d.
Embodiment 11
The tissue culture method of the bletilla striata:
Preparation seed germination medium: modified form MS medium+NAA0.5mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Preparation differential medium: modified form MS medium+6-BA2.0mg/L+NAA0.2mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Modified form MS medium is as embodiment 6.
Root media is as embodiment 6.
First by seed germination 30 days, and then be transferred to differential medium, cultivate 30 days, finally move to root media and cultivate 60 days, above three process condition of culture are identical: temperature 25 DEG C, intensity of illumination 1500lx, light application time 10h/d.
Embodiment 12
The tissue culture method of the bletilla striata:
Preparation seed germination medium: modified form MS medium+NAA0.6mg/L+30g/L murphy juice+30g/L sucrose+4.5g/L agar powder.
Preparation differential medium: modified form MS medium+6-BA3.0mg/L+NAA0.3mg/L+30g/L murphy juice+30g/L sucrose+4.5g/L agar powder.
Modified form MS medium is as embodiment 6.
Root media is as embodiment 6.
First by seed germination 30 days, and then be transferred to differential medium, cultivate 30 days, finally move to root media and cultivate 60 days, above three process condition of culture are identical: temperature 25 DEG C, intensity of illumination 1500lx, light application time 10h/d.
Embodiment 13
The tissue culture method of the bletilla striata:
Preparation seed germination medium: MS medium+NAA0.5mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Preparation differential medium: MS medium+6-BA2.0mg/L+NAA0.2mg/L+40g/L murphy juice+40g/L sucrose+4.3g/L agar powder.
Root media is as embodiment 4.
First by seed germination 30 days, and then be transferred to differential medium, cultivate 30 days, finally move to root media and cultivate 60 days, above three process condition of culture are identical: temperature 25 DEG C, intensity of illumination 1500lx, light application time 10h/d.
Experiment:
The group training result of Evaluation operation example 9-13, and by prior art as a control group.
The tissue culture method of control group: seed germination medium: MS+NAA0.5mg/L+ murphy juice 40g/L+ sucrose 40g/L+ agar powder, cultivates 30 days, is transferred in differential medium and cultivates.Differential medium: MS+6-BA2.0mg/L+NAA0.2mg/L+ murphy juice 40g/L+ sucrose 40g/L+ agar powder, cultivates 30 days, is transferred in root media and cultivates.Root media: MS+6-BA0.1mg/L+NAA1.0mg/L+ murphy juice 40g/L+ bananas juice 70g/L+ sucrose 40g/L+ agar.In all medium, agar powder consumption is 4.4g/L.Cultivate 80-90 days bottle outlets to transplant.
Result is as shown in table 4 and Fig. 1-4.Wherein, Fig. 1 is that embodiment 10 is at the bletilla striata seedling of culture of rootage after one month, Fig. 2 be control group at the bletilla striata seedling of culture of rootage after one month, Fig. 3 be embodiment 10 at culture of rootage bletilla striata seedling after two months, Fig. 4 is that control group is at culture of rootage bletilla striata seedling after two months.
The growing state of table 4 bletilla striata seedling
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1. for a root media for bletilla striata group training, it is characterized in that, comprise nutriment and sawdust, and often liter of described root media is containing 160-180g sawdust.
2. the root media for the training of bletilla striata group according to claim 1, it is characterized in that, described nutriment comprises: the MS medium of original concentration, the 0.1-2.0mg/L basic element of cell division and 0.1-1.0mg/L methyl α-naphthyl acetate.
3. the root media for the training of bletilla striata group according to claim 1, it is characterized in that, described nutriment comprises: modified form MS medium, the 0.1-2.0mg/L basic element of cell division and 0.1-1.0mg/L methyl α-naphthyl acetate;
The formula of described modified form MS medium is: potassium nitrate 1890-1900mg/L, ammonium nitrate 1650-1660mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, potassium dihydrogen phosphate 165-170mg/L, potassium iodide 0.75-0.83mg/L, boric acid 5-6.2mg/L, four water manganese sulphate 22.3-24mg/L, white vitriol 8.6-10mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.03mg/L, cobalt chloride 0.025-0.03mg/L, ferrous sulfate 27.8-30mg/L, ethylenediamine tetra-acetic acid 24.3-26mg/L, inositol 95-100mg/L, nicotinic acid 0.8-1mg/L, puridoxine hydrochloride 0.8-1mg/L, nicotinic acid thiamine 0.8-1mg/L, with glycine 3-4mg/L, pH is 5.8-6.0.
4. according to Claims 2 or 3 for the bletilla striata group training root media, it is characterized in that, described nutriment also comprise following in one or more: 60-70g/L bananas juice, 30-40g/L murphy juice, 30-40g/L sucrose.
5. the root media for the training of bletilla striata group according to Claims 2 or 3, it is characterized in that, the concentration of the basic element of cell division is 0.1-0.2mg/L, and the concentration of methyl α-naphthyl acetate is preferably 0.8-1.0mg/L, and the basic element of cell division preferably selects 6-BA.
6. the root media for the training of bletilla striata group according to claim 3, it is characterized in that, the formula of described modified form MS medium is: potassium nitrate 1895-1900mg/L, ammonium nitrate 1650-1655mg/L, calcium chloride dihydrate 440-442mg/L, epsom salt 370-373mg/L, potassium dihydrogen phosphate 165-170mg/L, potassium iodide 0.8-0.83mg/L, boric acid 5.5-6.2mg/L, four water manganese sulphate 22.3-23.6mg/L, white vitriol 8.6-10mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.03mg/L, cobalt chloride 0.028-0.03mg/L, ferrous sulfate 27.8-29mg/L, ethylenediamine tetra-acetic acid 24.3-25mg/L, inositol 98-100mg/L, nicotinic acid 0.8-0.9mg/L, puridoxine hydrochloride 0.8-0.9mg/L, nicotinic acid thiamine 0.8-0.9mg/L, with glycine 3-3.5mg/L, pH is 5.8-6.0.
7. the root media for the training of bletilla striata group according to claim 1, is characterized in that, described sawdust is the sawdust through liquor natrii hypochloritis's cleaning.
8. the tissue culture method of the bletilla striata, is characterized in that, comprises the following steps:
Making bletilla striata seeds through sprouting and differentiation, transferring in the root media described in any one of claim 1-3 and carrying out culture of rootage.
9. the tissue culture method of the bletilla striata according to claim 8, is characterized in that, described germination process medium used is: modified form MS medium, 0.5-0.6mg/L methyl α-naphthyl acetate and 4.3-4.5g/L agar powder;
The formula of described modified form MS medium is: potassium nitrate 1895-1900mg/L, ammonium nitrate 1650-1655mg/L, calcium chloride dihydrate 440-442mg/L, epsom salt 370-373mg/L, potassium dihydrogen phosphate 165-170mg/L, potassium iodide 0.8-0.83mg/L, boric acid 5.5-6.2mg/L, four water manganese sulphate 22.3-23.6mg/L, white vitriol 8.6-10mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.03mg/L, cobalt chloride 0.028-0.03mg/L, ferrous sulfate 27.8-29mg/L, ethylenediamine tetra-acetic acid 24.3-25mg/L, inositol 98-100mg/L, nicotinic acid 0.8-0.9mg/L, puridoxine hydrochloride 0.8-0.9mg/L, nicotinic acid thiamine 0.8-0.9mg/L, with glycine 3-3.5mg/L, pH is 5.8-6.0,
Preferably, the medium that described germination process is used also comprises 30-40g/L murphy juice, 30-40g/L sucrose.
10. the tissue culture method of the bletilla striata according to claim 8, is characterized in that, described atomization medium used is: modified form MS medium, the 2.0-3.0mg/L basic element of cell division, 0.2-0.3mg/L methyl α-naphthyl acetate and 4.3-4.5g/L agar powder;
The formula of described modified form MS medium is: potassium nitrate 1895-1900mg/L, ammonium nitrate 1650-1655mg/L, calcium chloride dihydrate 440-442mg/L, epsom salt 370-373mg/L, potassium dihydrogen phosphate 165-170mg/L, potassium iodide 0.8-0.83mg/L, boric acid 5.5-6.2mg/L, four water manganese sulphate 22.3-23.6mg/L, white vitriol 8.6-10mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.03mg/L, cobalt chloride 0.028-0.03mg/L, ferrous sulfate 27.8-29mg/L, ethylenediamine tetra-acetic acid 24.3-25mg/L, inositol 98-100mg/L, nicotinic acid 0.8-0.9mg/L, puridoxine hydrochloride 0.8-0.9mg/L, nicotinic acid thiamine 0.8-0.9mg/L, with glycine 3-3.5mg/L, pH is 5.8-6.0,
Preferably, the medium that described germination process is used also comprises 30-40g/L murphy juice, 30-40g/L sucrose.
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| CN201510593409.3A CN105028215B (en) | 2015-09-17 | 2015-09-17 | It is a kind of for the root media of bletilla striata tissue culture and the tissue culture method of the bletilla striata |
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| CN201510593409.3A CN105028215B (en) | 2015-09-17 | 2015-09-17 | It is a kind of for the root media of bletilla striata tissue culture and the tissue culture method of the bletilla striata |
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| CN105028215A true CN105028215A (en) | 2015-11-11 |
| CN105028215B CN105028215B (en) | 2017-11-14 |
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Cited By (6)
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| CN106613264A (en) * | 2016-12-29 | 2017-05-10 | 铜仁昱丰农业发展有限公司 | Bletilla striata tissue culture seedling transplantation and acclimation technology |
| CN106973789A (en) * | 2017-03-29 | 2017-07-25 | 师宗县福禄农业科技开发有限公司 | A kind of root media and its method for culturing seedlings of bletilla striata tissue culture |
| CN108094209A (en) * | 2017-12-28 | 2018-06-01 | 临沧市云瑞堂生物科技有限公司 | A kind of bletilla striata quickly breeds the tissue culture method of seedling |
| CN108124704A (en) * | 2017-12-27 | 2018-06-08 | 康美药业（文山）药材种植管理有限公司 | A kind of oldenlandia diffusa subculture medium and preparation method thereof |
| CN109089851A (en) * | 2018-06-21 | 2018-12-28 | 四川千草生物技术股份有限公司 | A kind of bletilla striata live streaming fast breeding method |
| CN110283775A (en) * | 2019-07-12 | 2019-09-27 | 遵义医科大学 | Secondary metabolite synthetic method in a kind of promotion bletilla suspended culture cell |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613264A (en) * | 2016-12-29 | 2017-05-10 | 铜仁昱丰农业发展有限公司 | Bletilla striata tissue culture seedling transplantation and acclimation technology |
| CN106613264B (en) * | 2016-12-29 | 2020-04-17 | 铜仁昱丰农业发展有限公司 | Bletilla striata tissue culture seedling transplanting domestication technology |
| CN106973789A (en) * | 2017-03-29 | 2017-07-25 | 师宗县福禄农业科技开发有限公司 | A kind of root media and its method for culturing seedlings of bletilla striata tissue culture |
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| CN108094209A (en) * | 2017-12-28 | 2018-06-01 | 临沧市云瑞堂生物科技有限公司 | A kind of bletilla striata quickly breeds the tissue culture method of seedling |
| CN109089851A (en) * | 2018-06-21 | 2018-12-28 | 四川千草生物技术股份有限公司 | A kind of bletilla striata live streaming fast breeding method |
| CN110283775A (en) * | 2019-07-12 | 2019-09-27 | 遵义医科大学 | Secondary metabolite synthetic method in a kind of promotion bletilla suspended culture cell |
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Effective date of registration: 20190305 Address after: 650000 6th Floor, Block A, Sunshine Building, 999 Kegao Road, Kunming City, Yunnan Province Patentee after: Kangmei Pharmaceutical (Kunming) Germplasm Resources Co., Ltd. Address before: 663000 Wenshan Zhuang and Miao Autonomous Prefecture, Yunnan Province, No. 4-21, Wenshan Biovalley of Chinese Traditional Medicine, South Kaihua Road, Wenshan City Patentee before: Kangmei Pharmaceutical (Wenshan) medicinal material planting Management Co., Ltd. |