CN106613264A - Bletilla striata tissue culture seedling transplantation and acclimation technology - Google Patents
Bletilla striata tissue culture seedling transplantation and acclimation technology Download PDFInfo
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- CN106613264A CN106613264A CN201611244428.6A CN201611244428A CN106613264A CN 106613264 A CN106613264 A CN 106613264A CN 201611244428 A CN201611244428 A CN 201611244428A CN 106613264 A CN106613264 A CN 106613264A
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- tissue culture
- sandy soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a bletilla striata tissue culture seedling transplantation and acclimation technology, which comprises the following steps: 1, preparing sand soil, the sand soil being one of yellow sand soil and black sand soil, frying-heating the sand soil to more than 80 DEG C by virtue of a caldron to evaporate moisture in the sand soil and kill parasite ova, cooling the sand soil to room temperature, and screening the sand soil once with a fine screen for later use; 2, spreading the prepared sand soil into bletilla striata tissue culture seedling bottles which are about 5cm high, the spread sand soil having a thickness capable of just covering the roots of seedlings; 3, placing the seedling bottles in step 2 in a tissue culture room to grow for 15 to 30d, and keeping the room temperature of 20 to 30 DEG C and the humidity of about 70 percent. According to the technology, the tissue culture seedlings are not required to be cleaned in an acclimation process, so that the roots of the seedlings are prevented from damage, meanwhile, manpower, chemicals and time are greatly saved, and complex steps for tissue culture seedling acclimation are simplified.
Description
Technical field
The present invention relates to bletilla striata training transplantation of seedlings acclimatization technology field, more particularly to a kind of bletilla tissue culture seedlings transplanting domestication skill
Art.
Background technology
Existing bletilla tissue culture seedlings need to clean the culture medium of plantlet in vitro root with clear water before transplantation, also want Jing
Cross sterilization, sprinkling root-growing agent, to place into wait in soil seedling and grow new root just can bury domestication, take and manually measure greatly, it is time-consuming compared with
It is long, seedling root can be damaged when seedling is cleaned, cause the seedling percent during transplanting low, up to 70% or so.For
This, it is proposed that a kind of bletilla tissue culture seedlings transplant acclimatization technology.
The content of the invention
Based on the technical problem that background technology is present, the present invention proposes a kind of bletilla tissue culture seedlings and transplants acclimatization technology.
The present invention proposes a kind of bletilla tissue culture seedlings and transplants acclimatization technology, comprises the steps：
S1, preparation sandy soil, the sandy soil are yellow sand or the one kind in black sandy soil；Sandy soil cauldron is made popular to more than 80 DEG C, is steamed
Send out the moisture in sandy soil and kill parasitic ovum, be cooled to room temperature, selected sandy soil brush one time with dusting cover, it is standby；
S2, ready sandy soil are sprinkled into grow in the high bletilla tissue culture seedlings bottles of 5cm or so, be sprinkled into the thickness of sandy soil by seedling
Root is covered；
S3, the seedling bottle in S2 is placed in tissue culture room grows 15-30d, keep room temperature at 20-30 DEG C, humidity is 70% or so；
S4, after the culture medium in plantlet in vitro bottle has been absorbed by sandy soil by bury domestication；
S5, prepare Laoshan soil and sheep (ox, horse etc. the also can) muck for fermenting, by Laoshan soil and muck according to 1：1 ratio is mixed
Close and lay after stirring on the ground as matrix, winter is equipped with ground hot line in the bottom surface of matrix；
S6, the whole bottle of the plantlet in vitro for having been absorbed culture medium by sandy soil are put into matrix, keep room temperature at 20-30 DEG C, and humidity is 60%
More than tamed；
S7, domestication time are 60-90d, and party's plantlet in vitro has after pseudobulb just can transplant.
Preferably, bletilla tissue culture seedlings are transplanted domestication process and are carried out in booth, booth outer side covers two-layer sunshade net, with
The passage of domestication time, one layer of sunshade net can be first taken off, subsequently removes other one layer of sunshade net；And by booth timely
Ventilation.
Preferably, in S5, by matrix point furrow, plantlet in vitro is planted on furrow；During matrix is made, can be in base
Woodland fertile soil, weeds straw decomposing soil are added in matter whipping process or adds a small amount of sawdust.
Present invention process process is simple, is easy to operation, during training, without cleaning plantlet in vitro, so will not be right
Seedling root causes to damage, due to seedling be directly by culture medium transition contact soil, so during hardening is transplanted into
Motility rate may be up to more than 95%, save substantial amounts of artificial, medicine and time, simplify the tedious steps of plantlet in vitro domestication.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1
A kind of bletilla tissue culture seedlings transplant acclimatization technology, comprise the steps：
S1, preparation sandy soil, the sandy soil are yellow sand or the one kind in black sandy soil；Sandy soil cauldron is made popular to 80 DEG C, is evaporated
Moisture in sandy soil simultaneously kills parasitic ovum, is cooled to room temperature, is selected sandy soil brush one time with dusting cover, standby；
S2, ready sandy soil are sprinkled into grow in the high bletilla tissue culture seedlings bottles of 5cm, be sprinkled into the thickness of sandy soil by seedling root
Cover；
S3, the seedling bottle in S2 is placed in tissue culture room grows 15d, keep room temperature at 22 DEG C, humidity is 70%；
S4, after the culture medium in plantlet in vitro bottle has been absorbed by sandy soil by bury domestication；
S5, prepare Laoshan soil and sheep (ox, horse etc. the also can) muck for fermenting, by Laoshan soil and muck according to 1：1 ratio is mixed
Close and lay after stirring on the ground as matrix, winter is equipped with ground hot line in the bottom surface of matrix；
S6, the whole bottle of the plantlet in vitro for having been absorbed culture medium by sandy soil are put into matrix, keep room temperature at 20 DEG C, and humidity is entered 60%
Row domestication；
S7, domestication time are 60d, and party's plantlet in vitro has after pseudobulb just can transplant.
Embodiment 2
A kind of bletilla tissue culture seedlings transplant acclimatization technology, comprise the steps：
S1, preparation sandy soil, the sandy soil are yellow sand or the one kind in black sandy soil；Sandy soil cauldron is made popular to 100 DEG C, is evaporated
Moisture in sandy soil simultaneously kills parasitic ovum, is cooled to room temperature, is selected sandy soil brush one time with dusting cover, standby；
S2, ready sandy soil are sprinkled into grow in the high bletilla tissue culture seedlings bottles of 6cm, be sprinkled into the thickness of sandy soil by seedling root
Cover；
S3, the seedling bottle in S2 is placed in tissue culture room grows 20d, keep room temperature at 23 DEG C, humidity is 73%；
S4, after the culture medium in plantlet in vitro bottle has been absorbed by sandy soil by bury domestication；
S5, prepare Laoshan soil and sheep (ox, horse etc. the also can) muck for fermenting, by Laoshan soil and muck according to 1：1 ratio is mixed
Close and lay after stirring on the ground as matrix, winter is equipped with ground hot line in the bottom surface of matrix；
S6, the whole bottle of the plantlet in vitro for having been absorbed culture medium by sandy soil are put into matrix, keep room temperature at 23 DEG C, and humidity is entered 63%
Row domestication；
S7, domestication time are 70d, and party's plantlet in vitro has after pseudobulb just can transplant.
Embodiment 3
A kind of bletilla tissue culture seedlings transplant acclimatization technology, comprise the steps：
S1, preparation sandy soil, the sandy soil are yellow sand or the one kind in black sandy soil；Sandy soil cauldron is made popular to 120 DEG C, is evaporated
Moisture in sandy soil simultaneously kills parasitic ovum, is cooled to room temperature, is selected sandy soil brush one time with dusting cover, standby；
S2, ready sandy soil are sprinkled into grow in the high bletilla tissue culture seedlings bottles of 7cm, be sprinkled into the thickness of sandy soil by seedling root
Cover；
S3, the seedling bottle in S2 is placed in tissue culture room grows 25d, keep room temperature at 250 DEG C, humidity is 75%；
S4, after the culture medium in plantlet in vitro bottle has been absorbed by sandy soil by bury domestication；
S5, prepare Laoshan soil and sheep (ox, horse etc. the also can) muck for fermenting, by Laoshan soil and muck according to 1：1 ratio is mixed
Close and lay after stirring on the ground as matrix, winter is equipped with ground hot line in the bottom surface of matrix；
S6, the whole bottle of the plantlet in vitro for having been absorbed culture medium by sandy soil are put into matrix, keep room temperature at 27 DEG C, and humidity is entered 65%
Row domestication；
S7, domestication time are 80d, and party's plantlet in vitro has after pseudobulb just can transplant.
Embodiment 4
A kind of bletilla tissue culture seedlings transplant acclimatization technology, comprise the steps：
S1, preparation sandy soil, the sandy soil are yellow sand or the one kind in black sandy soil；Sandy soil cauldron is made popular to 130 DEG C, is evaporated
Moisture in sandy soil simultaneously kills parasitic ovum, is cooled to room temperature, is selected sandy soil brush one time with dusting cover, standby；
S2, ready sandy soil are sprinkled into grow in the high bletilla tissue culture seedlings bottles of 8cm, be sprinkled into the thickness of sandy soil by seedling root
Cover；
S3, the seedling bottle in S2 is placed in tissue culture room grows 30d, keep room temperature at 30 DEG C, humidity is 77%；
S4, after the culture medium in plantlet in vitro bottle has been absorbed by sandy soil by bury domestication；
S5, prepare Laoshan soil and sheep (ox, horse etc. the also can) muck for fermenting, by Laoshan soil and muck according to 1：1 ratio is mixed
Close and lay after stirring on the ground as matrix, winter is equipped with ground hot line in the bottom surface of matrix；
S6, the whole bottle of the plantlet in vitro for having been absorbed culture medium by sandy soil are put into matrix, keep room temperature at 30 DEG C, and humidity is entered 68%
Row domestication；
S7, domestication time are 90d, and party's plantlet in vitro has after pseudobulb just can transplant.
In the present invention, bletilla tissue culture seedlings are transplanted domestication process and are carried out in booth, booth outer side covers two-layer sunshade net,
With the passage of domestication time, one layer of sunshade net can be first taken off, subsequently remove other one layer of sunshade net；And booth is timely
Ventilation；In S5, by matrix point furrow, plantlet in vitro is planted on furrow；During matrix is made, can stir in matrix
Woodland fertile soil, weeds straw decomposing soil are added during mixing or adds a small amount of sawdust, can be offer during seedling regrowth
Nutrient.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any those familiar with the art the invention discloses technical scope in, technology according to the present invention scheme and its
Inventive concept equivalent or change in addition, all should be included within the scope of the present invention.
Claims (3)
1. a kind of bletilla tissue culture seedlings transplant acclimatization technology, it is characterised in that comprise the steps：
S1, preparation sandy soil, the sandy soil are yellow sand or the one kind in black sandy soil；Sandy soil cauldron is made popular to more than 80 DEG C, is steamed
Send out the moisture in sandy soil and kill parasitic ovum, be cooled to room temperature, selected sandy soil brush one time with dusting cover, it is standby；
S2, ready sandy soil are sprinkled into grow in the high bletilla tissue culture seedlings bottles of 5cm or so, be sprinkled into the thickness of sandy soil by seedling
Root is covered；
S3, the seedling bottle in S2 is placed in tissue culture room grows 15-30d, keep room temperature at 20-30 DEG C, humidity is 70% or so；
S4, after the culture medium in plantlet in vitro bottle has been absorbed by sandy soil by bury domestication；
S5, prepare Laoshan soil and sheep (ox, horse etc. the also can) muck for fermenting, by Laoshan soil and muck according to 1：1 ratio is mixed
Close and lay after stirring on the ground as matrix, winter is equipped with ground hot line in the bottom surface of matrix；
S6, the whole bottle of the plantlet in vitro for having been absorbed culture medium by sandy soil are put into matrix, keep room temperature at 20-30 DEG C, and humidity is 60%
More than tamed；
S7, domestication time are 60-90d, and party's plantlet in vitro has after pseudobulb just can transplant.
2. bletilla tissue culture seedlings according to claim 1 transplant acclimatization technology, it is characterised in that bletilla tissue culture seedlings transplant domestication
Process is carried out in booth, booth outer side covers two-layer sunshade net, with the passage of domestication time, can first take one layer of screening off
Screened postive, subsequently removes other one layer of sunshade net；And by booth timely ventilation.
3. bletilla tissue culture seedlings according to claim 1 transplant acclimatization technology, it is characterised in that in S5, by matrix point furrow,
Plantlet in vitro is planted on furrow；During matrix is made, woodland fertile soil, weeds straw can be added in matrix whipping process
Stalk become thoroughly decomposed soil or add a small amount of sawdust.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611244428.6A CN106613264B (en) | 2016-12-29 | 2016-12-29 | Bletilla striata tissue culture seedling transplanting domestication technology |
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|---|---|---|---|
| CN201611244428.6A CN106613264B (en) | 2016-12-29 | 2016-12-29 | Bletilla striata tissue culture seedling transplanting domestication technology |
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| CN106613264A true CN106613264A (en) | 2017-05-10 |
| CN106613264B CN106613264B (en) | 2020-04-17 |
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| CN201611244428.6A Expired - Fee Related CN106613264B (en) | 2016-12-29 | 2016-12-29 | Bletilla striata tissue culture seedling transplanting domestication technology |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182788A (en) * | 2017-07-11 | 2017-09-22 | 中国科学院武汉植物园 | The propagation method of bletilla seedling |
| CN107211676A (en) * | 2017-05-26 | 2017-09-29 | 黄山峰源生物科技有限公司 | The acclimation method of bletilla tissue culture seedlings |
| CN111133960A (en) * | 2020-02-20 | 2020-05-12 | 江苏农林职业技术学院 | Transplanting method of clematis foraging tissue culture seedlings |
Citations (4)
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| CN1586140A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Soil hardening off for tissue cultured poplar seedling |
| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN105028215A (en) * | 2015-09-17 | 2015-11-11 | 康美药业（文山）药材种植管理有限公司 | Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
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2016
- 2016-12-29 CN CN201611244428.6A patent/CN106613264B/en not_active Expired - Fee Related
Patent Citations (4)
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| CN1586140A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Soil hardening off for tissue cultured poplar seedling |
| CN104604497A (en) * | 2015-01-28 | 2015-05-13 | 李泽诚 | Bletilla striata tissue culture seedling exercising and cultivation method |
| CN105028215A (en) * | 2015-09-17 | 2015-11-11 | 康美药业（文山）药材种植管理有限公司 | Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211676A (en) * | 2017-05-26 | 2017-09-29 | 黄山峰源生物科技有限公司 | The acclimation method of bletilla tissue culture seedlings |
| CN107182788A (en) * | 2017-07-11 | 2017-09-22 | 中国科学院武汉植物园 | The propagation method of bletilla seedling |
| CN111133960A (en) * | 2020-02-20 | 2020-05-12 | 江苏农林职业技术学院 | Transplanting method of clematis foraging tissue culture seedlings |
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| CN106613264B (en) | 2020-04-17 |
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