CN102986444A - Method for enabling woody plant material to form adventitious root and grow into own-rooted seedling - Google Patents
Method for enabling woody plant material to form adventitious root and grow into own-rooted seedling Download PDFInfo
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- CN102986444A CN102986444A CN2012105851129A CN201210585112A CN102986444A CN 102986444 A CN102986444 A CN 102986444A CN 2012105851129 A CN2012105851129 A CN 2012105851129A CN 201210585112 A CN201210585112 A CN 201210585112A CN 102986444 A CN102986444 A CN 102986444A
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Abstract
The invention provides a method for enabling a woody plant material to form an adventitious root and grow into an own-rooted seedling. The method includes selecting a full annual lignified branch or a cryptophytic perennial branch of the woody plant, maintaining the humidity needed for the survival of the branch, keeping germinal shoots etiolation and delaying lignification through controlling light, and inducing lateral twigs to root. The method is convenient to operate, low-carbon, environment-friendly, low in cost, and capable of enabling tree species difficult to obtain own-rooted seedlings through existing methods to obtain own-rooted seedlings easily and increasing the material selecting range of own-rooted seedling of tree species easy to cut.
Description
Technical field
The present invention relates to a kind of method of impelling the woody plant material to form adventive root and cultivating into own-rooted tree, relate in particular to the method that a kind of woody plant material that impels difficulty to take root forms adventive root and cultivates into own-rooted tree, belong to woody plant propagation technique field.
Background technology
The superiority that own-rooted tree has grafting and seedling to replace in production and scientific research not only has the advantage that keeps maternal good genetic character, and good uniformity is arranged.
At present, the method for woody plant production own-rooted tree mainly contains cuttage, press strip, plant division and tissue and cultivates, and former three is wide-spread, ancient conventional method, uses till today always, and latter is the new method that just occurred in 20th century.General cuttage, press strip and plant division are easy and simple to handle, do not need special installation, and simple and easy to do, expense is lower, but only are suitable for producing easily the plant of adventive root; The difficult plant that takes root generally adopt the band sectional leaf cutting of semi-lignified or claim green branch appropriateness shade or full exposure atomizing condition under cuttage obtain own-rooted tree, difficulty and expense all increase to some extent; More difficult take root then will be by tissue culture technique, need strict sterile working, equipment needed thereby condition and staff condition are all higher, expense is also higher; Some woody plants are more arranged, there is no at present the ripe method that effectively produces own-rooted tree.
The ability that the vegetable material that degree of lignification is lower forms adventive root is stronger, is difficult to later on survive but not lignified woody plant material leaves parent, and just because of this, the difficult plant that takes root is adopted semi-lignified cutting cuttage usually, or passes through tissue culture propagation.Stored nutrient was less during semi-lignified was transplanted a cutting, and Chang Buneng satisfies the nutrient consumption in the branch rooting process, needed to keep an amount of blade and be under the solar radiation, by self photosynthesis manufacturing nutrient, kept the nutritional need in the rooting process.But because there not being to absorb the root system of moisture, just face in the sun the withered danger of loss of water thereby transplant a cutting to expose for a long time, therefore must reduce moisture loss by suitable spraying, the water balance that maintenance is transplanted a cutting.This is so that semi-lignified cuttage process is more complicated than hard cuttage, and conditional request is also higher.The common degree of lignification of the explant that tissue culture adopts is lower, need to from the outside, namely draw various nutriments in the medium.Nutritious in the medium, also created desirable condition for the breeding of microorganism, therefore must microorganism be isolated in outside the culture environment by sterile working.Aseptic condition is the main cause that tissue culture propagation is loaded down with trivial details, cost is high.
Up to now, the characteristics that can utilize low degree of lignification branch easily to take root do not need again higher environmental condition, inexpensive method easy and simple to handle to have not yet to see report.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of easy new method of obtaining the woody plant own-rooted tree.
Adopt following technical scheme for solving this technology:
(1) chooses substantial full annual lignified branch, or the perennial branch of the woody plant that kryptoblast is arranged, the stem section that is cut into length 10～20cm is placed in the plastics plastic shopping bag as cutting, use the liver moss moisturizing of soaking in the bag, and to keep in the bag 2/3 space being arranged be air, keep in Dark Place, jede Woche is opened the inspection of plastics plastic shopping bag and is breathed freely once spraying and moisturizing;
(2) the late March lateral bud begins to sprout successively.Before and after lateral bud or kryptoblast are sprouted the stem section of preserving is tiled on draining ventilative good chesson, liver moss or other mixed-matrixes, continues moisturizing, lucifuge.Tender tip length reaches 3～5cm at the beginning of 4 months, begins to penetrate faint scattered light this moment;
(3) remove too much shoot, only keep 2 tender tips of new life on every root cutting
(4) high-voltage electrostatic field (HVEF) processes: intensity is 45KV/cm, time 2 h;
(5) root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, ABT0.5 part, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, 0.5 part of vitamin B1, with the dilution ratio of 600mg: 1L, soak cutting bottom 2h;
(6) will take root and transplant a cutting move in the flowerpot, the matrix of flowerpot was preferably: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3: 1: 2: 1, the thick 10cm that reaches of described matrix mixing place mat, and cover newborn adventive root and the tender tip base portion of taking root with matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks began to be exposed under the natural sunlight after transplanting, water as required.
The beneficial effect of the relative prior art of the present invention is:
The application's method takes full advantage of the characteristics that characteristics that lignification branch not takes root easily and 1 year to perennial lignification branch has stored nutrient and nutrient can be provided continually for the tender tip of new life, thereby breeds own-rooted tree so that generate adventive root.Main advantage has:
1 plant growth regulator is a kind of ectogenic non-nutritive chemical substance, it can conduct to site of action usually in plant corpus, just can promote or suppress some link in the plant life process with lower concentration, make it to the wish development that meets the human needs; Plant establishment agent traditional in the prior art is very limited for the rooting effect that promotes plant, especially for some plant with difficult rooting, such as ornamental trees and shrubs etc., effect is not remarkable, the present application people draws root-inducing powder prescription of the present invention by lot of experiments, it is easy to use, can improve improving activity of root system, promote root growth, improve the chlorophyll content of transplanting plant, strengthen photosynthesis, and the auxin rational proportion that contains, Coordinated Interaction has functions such as promoting the root hair cell division, the growing of Effective Raise root hair, prolong the suitable phase of plant transplantation, improve and transplant rear new root number and the long speed of new root, obviously shorten seedling-slowing stage, tachyauxesis.
Be checking root-growing agent effect of the present invention, establish following three processing take the woody plant apocarya as research object:
Process 1: commercially available powerful root-inducing powder; Step is with embodiment 1
Process 2: commercially available ABT root-inducing powder soaks; Step is with embodiment 1
Process 3: root-inducing powder of the present invention soaks, according to the processing mode of embodiment 1.
Table 1: root-inducing powder is on the impact of root length, radical growth rate and survival rate
| ? | The long growth rate of root | The radical growth rate | Survival rate |
| Process 1 | 9.4％ | 10％ | 49％ |
| Process 2 | 19.5％ | 13.7％ | 61％ |
| Process 3 | 26.3％ | 21.5％ | 75％ |
2 utilize the high-voltage electrostatic field to process the cuttage branch, and the motion that it has affected permeability and other polar molecule of film has improved absorption and the transhipment physical capacity of cell, thereby can promote the growth organized.It not only can strengthen improving activity of root system, increase the biomass of root, improve cuttage survival rate, promote growth of seedling, but also can strengthen premunition, kipeming high yield, yet, utilize the high-voltage electrostatic field to process the cuttage plastid, different kind numerical value differ larger, and different intensity can produce different effects with ageing, if it is excessive to process electric-field intensity, or the processing time is long, meeting is so that plant cell comes to harm even death, and electric-field intensity is too small or the time is too short, can cause again treatment effect not obvious, it is 45KV/cm that the present application people draws for apocarya branch employing intensity through creative work, the treatment effect of time 2 h is best, the root growth rate, cuttage survival rate, vitality index numerical value is maximum, conductivity values is minimum, and every physical signs is extreme value.
Table 2: single-factor experimental verification high-voltage electrostatic field is on the impact of apocarya shoot growth
3 Screening matrix embodiment
Horse liver soil is the distinctive a kind of soil in THE LOWER YANGTZE VALLEY area, and Sha Xingqiang does and can consolidate root, but wet water conservation, its shortcoming is that water permeable ability is strong, and dehydration is too fast, air-dry easily, and single use can make the base portion dehydration withered.Peat soil has good permeability and larger salt balance ability, perlite and vermiculite suction, ventilative, the present application people is through creative work for many years, screen the contrast experiment, from a large amount of cutting mediums, screen the liver soil of going into action, vermiculite, peat, these four kinds of matrix of perlite according to 3: 1: 2: 1 part by weight is combined with, do mutually mutual induction between each matrix, reach the effect of ventilative, moisturizing, solid root.
The invention discloses the method that some are difficult to according to a conventional method to obtain the woody plant material formation adventive root of seeds and breed own-rooted tree, greatly increased the scope of selecting material that conventional method can obtain the seeds cottage propagation of own-rooted tree, be not limited only to semi-lignified branch and annotinous branch, even can with perennial tree section, the obsolete material of pruning be fully utilized.
Embodiment
Embodiment one
Chose 20 of the stem sections that Juglandaceae cultivated plant apocarya (Carya illinoensis) dormant wood is cut into length 20cm, diameter 10～15mm on January 25th, 2011, be placed in the plastics plastic shopping bag as cutting, the liver moss moisturizing of soaking in the bag, and to keep in the bag 2/3 space being arranged be air, keeps in Dark Place.The late March lateral bud begins to sprout successively.Tender tip length reaches 3～5cm at the beginning of 4 months, begins to penetrate faint scattered light this moment; Remove too much shoot, only keep 2 tender tips of new life on every root cutting.April 26, high-voltage electrostatic field (HVEF) processes: intensity is 45KV/cm, time 2 h; Root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, 0.5 part of ABT7 root-inducing powder, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, 0.5 part of vitamin B1, with the dilution ratio of 600mg: 1L, soak cutting bottom 2h; To take root for transplanting a cutting and move in the flowerpot, flowerpot matrix was: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3: 1: 2: 1, the thick 10cm that reaches of matrix mixing place mat, and cover newborn adventive root and the tender tip base portion of taking root with matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks began to be exposed under the natural sunlight after transplanting, water as required.
Had no still that the tender tip that the cutting of taking root sends out rots afterwards successively on May 7 or withered.December 31 ended, seedling 15 strains, and total planting percent reaches 75%, average height of seedling 29.4cm, base diameter average out to 0.97cm.
Embodiment two
On February 7th, 2012, choose rose family wild plant rubus coreanus (Rubus coreanus) stolon, be cut into 50 of the long stem sections of 15cm, be placed in the plastics plastic shopping bag as cutting, the liver moss moisturizing of soaking in the bag, and to keep in the bag 2/3 space being arranged be air, keeps in Dark Place.Jede Woche is opened the inspection of plastics plastic shopping bag and is breathed freely once, spraying and moisturizing, and the late March lateral bud begins to sprout successively.Treat that tender tip length reaches 3cm, begin to penetrate faint scattered light this moment; Remove too much shoot, only keep 2 tender tips of new life on every root cutting.Process high-voltage electrostatic field on April 3 (HVEF): intensity is 45KV/cm, time 2 h; Root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, 0.5 part of ABT7 root-inducing powder, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, vitaminB10 .5 part, with the dilution ratio of 600mg: 1L, soak cutting bottom 2h; To take root for transplanting a cutting and move in the flowerpot, flowerpot matrix was: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3: 1: 2: 1, the thick 10cm that reaches of matrix mixing place mat, and cover newborn adventive root and the tender tip base portion of taking root with matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks began to be exposed under the natural sunlight after transplanting, water as required.Daily inspection in Mays 8 sees that 8 strains do not wither 42 survivals because taking root.Still have by the end of November 42 strains to survive, survival rate reaches 84%.
Although, above the present invention is described in detail with a general description of the specific embodiments, but on basis of the present invention, can make some modifications or improvements it, or the enforcement of the seeds outside case study on implementation this method, this will be apparent to those skilled in the art.Therefore, these that make are without departing from theon the basis of the spirit of the present invention revised, and the expansion of improvement or scope all belongs to the scope of protection of present invention.
Claims (2)
1. method of impelling the woody plant material to form adventive root and breeding own-rooted tree is characterized in that:
(1) chooses lignified annual extremely perennial branch 1-2 month, be cut into the stem section of length 15-20cm, be placed in the plastics plastic shopping bag as cutting, use the liver moss moisturizing of soaking in the bag, and to keep in the bag 2/3 space being arranged be air, keep in Dark Place, jede Woche is opened the inspection of plastics plastic shopping bag and is breathed freely once spraying and moisturizing;
(2) the late March lateral bud begins to sprout successively, and tender tip length reaches 3～5cm at the beginning of 4 months, begins to penetrate faint scattered light this moment;
(3) remove too much shoot, only keep 2 tender tips of new life on every root cutting;
(4) high-voltage electrostatic field (HVEF) processes: intensity is 45KV/cm, time 2 h;
(5) root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, ABT0.5 part, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, vitamin B1 are 0.5 part; With the 600mg root-inducing powder: the dilution proportion of 1L water, then soak and transplant a cutting bottom 2h;
(6) will take root and transplant a cutting move in the flowerpot, the matrix of flowerpot was preferably: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3: 1: 2: 1, the thick 10cm that reaches of described matrix mixing place mat, and cover newborn adventive root with matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks began to be exposed under the natural sunlight after transplanting, water as required.
2. method according to claim 1 is characterized in that, the awkward woody plant material of taking root of described woody plant material is preferably apocarya, rubus coreanus.
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Cited By (14)
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| CN103238492A (en) * | 2013-05-13 | 2013-08-14 | 江苏省中国科学院植物研究所 | Process for facilitating sprouting of adventitious roots of apocarya branches |
| CN103299812A (en) * | 2013-06-20 | 2013-09-18 | 南京绿宙薄壳山核桃科技有限公司 | Apocarya hardwood cutting seedling raising method |
| CN104396656A (en) * | 2014-09-23 | 2015-03-11 | 常州市金土地农牧科技服务有限公司 | Yield-increasing method of pecans |
| CN104904499A (en) * | 2014-05-09 | 2015-09-16 | 常州市金土地农牧科技服务有限公司 | Method for grafting caryaillinoinensis |
| CN105210865A (en) * | 2015-09-17 | 2016-01-06 | 重庆市畜牧科学院 | The method that U.S.'s vetch high frequency regenerating system is set up |
| CN105766513A (en) * | 2016-02-25 | 2016-07-20 | 孙阳 | Walnut own-rooted seedling breeding method |
| CN105830718A (en) * | 2016-04-13 | 2016-08-10 | 江苏省中国科学院植物研究所 | Cutting propagation method for Torreya jackii |
| CN105993467A (en) * | 2016-05-20 | 2016-10-12 | 芜湖欧标农业发展有限公司 | Cutting seedling raising method for erythrina corallodendron |
| CN106034584A (en) * | 2016-05-20 | 2016-10-26 | 芜湖欧标农业发展有限公司 | Cuttage seedling propagation method for Erythrina cristagalli |
| CN107148860A (en) * | 2017-05-08 | 2017-09-12 | 茂名市绿凰农业发展有限公司 | It is a kind of that the engrafting method of the high-quality and efficient safety in production of crop is promoted based on high-voltage electrostatic field |
| CN107624398A (en) * | 2016-07-12 | 2018-01-26 | 郑德剑 | One planting fruit-trees high pressure branch propagation method |
| CN107646362A (en) * | 2017-09-27 | 2018-02-02 | 界首市五季果源家庭农场 | A kind of method of tribute chrysanthemum lateral bud children stem micro cuttage |
| CN110915446A (en) * | 2019-12-26 | 2020-03-27 | 广州建筑园林股份有限公司 | Ginger flower breeding method based on leaf and stem hidden bud germination promotion |
| CN112166847A (en) * | 2020-10-14 | 2021-01-05 | 四川七彩林科股份有限公司 | Efficient propagation method of snow fruits |
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| CN103238492B (en) * | 2013-05-13 | 2014-04-16 | 江苏省中国科学院植物研究所 | Process for facilitating sprouting of adventitious roots of apocarya branches |
| CN103238492A (en) * | 2013-05-13 | 2013-08-14 | 江苏省中国科学院植物研究所 | Process for facilitating sprouting of adventitious roots of apocarya branches |
| CN103299812A (en) * | 2013-06-20 | 2013-09-18 | 南京绿宙薄壳山核桃科技有限公司 | Apocarya hardwood cutting seedling raising method |
| CN103299812B (en) * | 2013-06-20 | 2014-04-16 | 南京绿宙薄壳山核桃科技有限公司 | Apocarya hardwood cutting seedling raising method |
| CN104904499A (en) * | 2014-05-09 | 2015-09-16 | 常州市金土地农牧科技服务有限公司 | Method for grafting caryaillinoinensis |
| CN104396656A (en) * | 2014-09-23 | 2015-03-11 | 常州市金土地农牧科技服务有限公司 | Yield-increasing method of pecans |
| CN105210865A (en) * | 2015-09-17 | 2016-01-06 | 重庆市畜牧科学院 | The method that U.S.'s vetch high frequency regenerating system is set up |
| CN105766513B (en) * | 2016-02-25 | 2019-02-01 | 孙阳 | A kind of walnut own-rooted tree mating system |
| CN105766513A (en) * | 2016-02-25 | 2016-07-20 | 孙阳 | Walnut own-rooted seedling breeding method |
| CN105830718A (en) * | 2016-04-13 | 2016-08-10 | 江苏省中国科学院植物研究所 | Cutting propagation method for Torreya jackii |
| CN106034584A (en) * | 2016-05-20 | 2016-10-26 | 芜湖欧标农业发展有限公司 | Cuttage seedling propagation method for Erythrina cristagalli |
| CN105993467A (en) * | 2016-05-20 | 2016-10-12 | 芜湖欧标农业发展有限公司 | Cutting seedling raising method for erythrina corallodendron |
| CN107624398A (en) * | 2016-07-12 | 2018-01-26 | 郑德剑 | One planting fruit-trees high pressure branch propagation method |
| CN107148860A (en) * | 2017-05-08 | 2017-09-12 | 茂名市绿凰农业发展有限公司 | It is a kind of that the engrafting method of the high-quality and efficient safety in production of crop is promoted based on high-voltage electrostatic field |
| CN107646362A (en) * | 2017-09-27 | 2018-02-02 | 界首市五季果源家庭农场 | A kind of method of tribute chrysanthemum lateral bud children stem micro cuttage |
| CN110915446A (en) * | 2019-12-26 | 2020-03-27 | 广州建筑园林股份有限公司 | Ginger flower breeding method based on leaf and stem hidden bud germination promotion |
| CN112166847A (en) * | 2020-10-14 | 2021-01-05 | 四川七彩林科股份有限公司 | Efficient propagation method of snow fruits |
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