CN102511391A - Hyacinthus orientalis L. in-vitro rapid propagation method - Google Patents
Hyacinthus orientalis L. in-vitro rapid propagation method Download PDFInfo
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- CN102511391A CN102511391A CN201110404105XA CN201110404105A CN102511391A CN 102511391 A CN102511391 A CN 102511391A CN 201110404105X A CN201110404105X A CN 201110404105XA CN 201110404105 A CN201110404105 A CN 201110404105A CN 102511391 A CN102511391 A CN 102511391A
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Abstract
The invention relates to a hyacinthus orientalis L. in-vitro rapid propagation method, and belongs to the field of biotechnology. The hyacinthus orientalis L. in-vitro rapid propagation method comprises the following steps of 1, selecting hyacinthus orientalis L. seed bulbs growing in an open field for 2 to 3 months, carrying out disinfection, and removing outer scales, 2, taking floret petals, flatwise putting the floret petals on a MS solid medium, and carrying out adventitious bud growth induction culture to obtain tissue blocks containing adventitious buds, 3, longitudinally dividing the tissue blocks containing adventitious buds, and carrying out bud elongation culture by a bud elongation medium, and 4, when length values of the adventitious buds obtained by the step 3 are in a range of 2 to 4 centimeters, cutting the adventitious buds, putting the cut adventitious buds into a rooting medium, and carrying out rooting induction culture to obtain hyacinthus orientalis L. seedlings. The young floret petals of hyacinthus orientalis L. are utilized as explants and directly regenerate a mass of adventitious buds and the adventitious buds further develop into regenerated seedlings. Compared with the existing tissue culture method utilizing scales as explants, the hyacinthus orientalis L. in-vitro rapid propagation method has high regeneration efficiency, a high budding rate of about 100% and a large average amount of buds regenerated by induction of each one explant, wherein the large average amount is about 20.
Description
Technical field
The present invention relates to the method for quickly breeding in a kind of biological technical field, be specifically related to a kind of hyacinth method for in-vitro rapid propagation.
Background technology
Hyacinth (Hyacinthus orientalis L.) is a Liliaceae Hyacinthus herbaceos perennial, originates in Mediterranean and South Africa, is the flowering bulb in spring that has sight of extensive use all over the world.China in last century the eighties from Dutch introduction of plant, but traditional propagation method is consuming time longer, reproductive efficiency is lower, plants costing an arm and a leg of ball simultaneously, can not satisfy the demand in market, has limited hyacinth plantation at home.Therefore, carrying out hyacinthine quick breeding through tissue culture is effective technical means, helps strengthening the popularization of hyacinth in China.
The hyacinth scale is the group training material of quick breeding the most commonly used.Research shows, hyacinth blade and the ovary formation seedling of also can regenerating.Though with the bulb be explant regenerate draw materials more convenient relatively; But because bulb carries disease germs seriously, usually cause sterilization not thorough, contamination phenomenon is very serious repeatedly in the incubation; Increase workload, and be that explant regeneration efficient is not very good with the scale.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, a kind of hyacinth method for in-vitro rapid propagation is provided.Method regeneration efficiency of the present invention increases, and pollution rate reduces, and has improved inductivity through regulating culture medium condition, has shortened the regeneration period, and this regenerating system is applicable to a plurality of hyacinth kinds.
The present invention realizes that through following technical scheme hyacinth method for in-vitro rapid propagation of the present invention comprises the steps: to get hyacinth Xiao Hua petal as explant, carries out regenerating and culturing, obtains the hyacinth seedling.
Preferably, described hyacinth method for in-vitro rapid propagation comprises the steps:
(a) get 2～3 months hyacinth kind ball of open country plantation, sterilization divests outer scale;
(b) strip the Xiao Hua petal, lie in the induced growth that carries out indefinite bud on the MS solid culture medium and cultivate, obtain to have the piece of tissue of indefinite bud clump;
(c) the vertical piece of tissue of dividing is carried out the bud elongation and is cultivated on the bud elongation medium;
When (d) indefinite bud is stretched to 2～4cm, puts into root media after the cutting and carry out the root induction cultivation, obtain the hyacinth seedling.
Preferably, in the step (b), said MS solid culture medium is MS+BA 2.0mg/L+NAA 1.0mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.
Preferably, in the step (b), said induced growth culture condition is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.
Preferably, in the step (c), said bud elongation medium is MS+BA0.5mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.
Preferably, in the step (c), said bud elongation culture condition is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.
Preferably, in the step (d), said root media is: MS+NAA0.5mg/L+ active carbon 1.0g/L, pH 5.8, sucrose 30g/L, agar 5g/L.
Preferably, in the step (d), said root induction culture condition is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.
The present invention has following beneficial effect: method of the present invention utilizes the tender small-flowered sheet of hyacinthine children to be explant; Directly a large amount of indefinite buds of regeneration obtain regrowth then, carry out tissue culture with the use scale as explant and compare; Method regeneration efficiency of the present invention increases; Bud ratio is about 100%, and on average the bud quantity of each explant induction regeneration can reach about 20, and pollution rate reduces; The situation that can not occur polluting repeatedly in the incubation can improve inductivity and shorten the regeneration period through regulating culture medium condition.
Embodiment
Below in conjunction with specific embodiment the present invention is elaborated.Following examples will help those skilled in the art further to understand the present invention, but not limit the present invention in any form.Should be pointed out that to those skilled in the art, under the prerequisite that does not break away from the present invention's design, can also make some distortion and improvement.These all belong to protection scope of the present invention.
Embodiment 1
Select good hyacinth kind Gypsy queen (Gipsy Queen) in planting about November in big Tanaka then, take kind of a ball mid-January next year;
Getting young tender small-flowered sheet is that explant carries out the bud inducing culture, and medium is MS+BA1.0mg/L+NAA0.5mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.Bud ratio can reach 70%, and on average the bud quantity of each explant induction regeneration is about 8.
Occur a large amount of bud clumps after 40 days on the petal sheet, after the cutting of bud clump, put into the bud elongation medium and cultivate, medium is MS+NAA0.5mg/L, pH5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.
Bud is elongated to 2-4cm after 30 days, and cutting bud clump is put into root media and cultivates, and root media is MS+NAA0.5mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.Obtain regrowth after bearing 3-4 bar root after 20 days.Transplant to little basin or open country behind the refining seedling, suitably shade a week, but the regrowth normal growth.There is not pollution condition repeatedly in the incubation.
Embodiment 2
Select good hyacinth kind Gypsy queen (Gipsy Queen) in planting about November in big Tanaka then, take kind of a ball mid-January next year;
Getting young tender small-flowered sheet is that explant carries out the bud inducing culture, and medium is MS+BA2.0mg/L+NAA1.0mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.Bud ratio can reach 100%, and on average the bud quantity of each explant induction regeneration is about 20.
Occur a large amount of bud clumps after 30 days on the petal sheet, after the cutting of bud clump, put into the bud elongation medium and cultivate, medium is MS+BA0.5mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.
Treat after 20 days that bud is elongated to 2-4cm, cutting bud clump is put into root media and cultivates, and root media is MS+NAA0.5mg/L+ active carbon 1.0g/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.Obtain regrowth after waiting to bear 3-4 bar root after 10 days.Transplant to little basin or open country behind the refining seedling, suitably shade a week, but the regrowth normal growth.There is not pollution condition repeatedly in the incubation.
Embodiment 3
Select good hyacinth kind Gypsy queen (Gipsy Queen) in planting about November in big Tanaka then, take kind of a ball mid-January next year;
Getting young tender small-flowered sheet is that explant carries out the bud inducing culture, and medium is MS+BA3.0mg/L+NAA2.0mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.Bud ratio can reach 80%, and on average the bud quantity of each explant induction regeneration is about 12.
Occur a large amount of bud clumps after 35 days on the petal sheet, after the cutting of bud clump, put into the bud elongation medium and cultivate, medium is MS+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.
Treat after 25 days that bud is elongated to 2-4cm, cutting bud clump is put into root media and cultivates, and root media is MS+NAA0.5mg/L+ active carbon 2.0g/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.Obtain regrowth after waiting to bear 3-4 bar root after 12 days.Transplant to little basin or open country behind the refining seedling, suitably shade a week, but the regrowth normal growth.There is not pollution condition repeatedly in the incubation.
Embodiment 4
Select good hyacinth kind Annamarie (Anna Marie) in planting about November in big Tanaka then, take kind of a ball mid-January next year;
Getting young tender small-flowered sheet is that explant carries out the bud inducing culture, and medium is MS+BA2.0mg/L+NAA1.0mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.Bud ratio can reach 100%, and on average the bud quantity of each explant induction regeneration is about 22.
Occur a large amount of bud clumps after 30 days on the petal sheet, after the cutting of bud clump, put into the bud elongation medium and cultivate, medium is MS+BA0.5mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.
Treat after 20 days that bud is elongated to 2-4cm, cutting bud clump is put into root media and cultivates, and root media is MS+NAA0.5mg/L+ active carbon 1.0g/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.Obtain regrowth after waiting to bear 3-4 bar root after 10 days.Transplant to little basin or open country behind the refining seedling, suitably shade a week, but the regrowth normal growth.There is not pollution condition repeatedly in the incubation.
Embodiment 5
Select good hyacinth kind starry sky (Sky Jacket) in planting about November in big Tanaka then, take kind of a ball mid-January next year;
Getting young tender small-flowered sheet is that explant carries out the bud inducing culture, and medium is MS+BA2.0mg/L+NAA1.0mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.Bud ratio can reach 100%, and on average the bud quantity of each explant induction regeneration is about 18.
Occur a large amount of bud clumps after 30 days on the petal sheet, after the cutting of bud clump, put into the bud elongation medium and cultivate, medium is MS+BA0.5mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.
Treat after 20 days that bud is elongated to 2-4cm, cutting bud clump is put into root media and cultivates, and root media is MS+NAA0.5mg/L+ active carbon 1.0g/L, and pH 5.8, sucrose 30g/L, agar 5g/L.Condition of culture is the same.Obtain regrowth after waiting to bear 3-4 bar root after 10 days.Transplant to little basin or open country behind the refining seedling, suitably shade a week, but the regrowth normal growth.There is not pollution condition repeatedly in the incubation.
In sum, method regeneration efficiency of the present invention increases, and pollution rate reduces, and has improved inductivity through regulating culture medium condition, has shortened the regeneration period, and this regenerating system is applicable to a plurality of hyacinth kinds.
Claims (8)
1. a hyacinth method for in-vitro rapid propagation is characterized in that, comprises the steps: to get hyacinth Xiao Hua petal as explant, carries out regenerating and culturing, obtains the hyacinth seedling.
2. hyacinth method for in-vitro rapid propagation as claimed in claim 1, its characteristic is being, is comprising the steps:
(a) get 2～3 months hyacinth kind ball of open country plantation, sterilization divests outer scale;
(b) strip the Xiao Hua petal, lie in the induced growth that carries out indefinite bud on the MS solid culture medium and cultivate, obtain to have the piece of tissue of indefinite bud clump;
(c) the vertical piece of tissue of dividing is carried out the bud elongation and is cultivated on the bud elongation medium;
When (d) indefinite bud is stretched to 2～4cm, puts into root media after the cutting and carry out the root induction cultivation, obtain the hyacinth seedling.
3. hyacinth method for in-vitro rapid propagation as claimed in claim 2, its characteristic are being that in the step (b), said MS solid culture medium is MS+BA 2.0mg/L+NAA 1.0mg/L, and pH 5.8, sucrose 30g/L, agar 5g/L.
4. hyacinth method for in-vitro rapid propagation as claimed in claim 2, its characteristic are being that in the step (b), said induced growth culture condition is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.
5. hyacinth method for in-vitro rapid propagation as claimed in claim 2, its characteristic are being that in the step (c), said bud elongation medium is MS+BA0.5mg/L+NAA1.0mg/L, pH5.8, sucrose 30g/L, agar 5g/L.
6. hyacinth method for in-vitro rapid propagation as claimed in claim 2, its characteristic are being that in the step (c), said bud elongation culture condition is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.
7. hyacinth method for in-vitro rapid propagation as claimed in claim 2, its characteristic are being that in the step (d), said root media is: MS+NAA0.5mg/L+ active carbon 1.0g/L, pH 5.8, sucrose 30g/L, agar 5g/L.
8. hyacinth method for in-vitro rapid propagation as claimed in claim 2, its characteristic are being that in the step (d), said root induction culture condition is: 25 ℃ of temperature, illumination: dark phase of 16 photophase/8.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891515A (en) * | 2014-04-15 | 2014-07-02 | 李蓁 | Planting method for common grape hyacinth flowers |
| CN104488715A (en) * | 2014-12-19 | 2015-04-08 | 浙江省农业科学院 | Method for carrying out bulb induction and plant regeneration of wide-leaf albuca namaquensis by flower buds |
| CN104521527A (en) * | 2015-01-06 | 2015-04-22 | 云南省农业科学院花卉研究所 | Rapid propagation method for hyacinths |
| CN105210877A (en) * | 2015-10-20 | 2016-01-06 | 韦丽 | A kind of Lilium brownii var viridulum method for quickly breeding |
| CN108834895A (en) * | 2018-07-14 | 2018-11-20 | 沿河后花园农业观光旅游综合开发有限公司 | A kind of lily method for in-vitro rapid propagation |
-
2011
- 2011-12-07 CN CN201110404105XA patent/CN102511391B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 刘勇刚等: "风信子的组织培养和植株再生", 《西北大学学报(自然科学版)》 * |
| 李玉萍等: "风信子组织培养研究进展", 《江苏农业科学》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891515A (en) * | 2014-04-15 | 2014-07-02 | 李蓁 | Planting method for common grape hyacinth flowers |
| CN103891515B (en) * | 2014-04-15 | 2015-11-04 | 李蓁 | Muscari botryoides seeds of flowering plants method for planting |
| CN104488715A (en) * | 2014-12-19 | 2015-04-08 | 浙江省农业科学院 | Method for carrying out bulb induction and plant regeneration of wide-leaf albuca namaquensis by flower buds |
| CN104488715B (en) * | 2014-12-19 | 2016-08-03 | 浙江省农业科学院 | A kind of method carrying out wide leaf spring grass bulb induction and plant regeneration by alabastrum |
| CN104521527A (en) * | 2015-01-06 | 2015-04-22 | 云南省农业科学院花卉研究所 | Rapid propagation method for hyacinths |
| CN104521527B (en) * | 2015-01-06 | 2017-01-11 | 云南省农业科学院花卉研究所 | Rapid propagation method for hyacinths |
| CN105210877A (en) * | 2015-10-20 | 2016-01-06 | 韦丽 | A kind of Lilium brownii var viridulum method for quickly breeding |
| CN108834895A (en) * | 2018-07-14 | 2018-11-20 | 沿河后花园农业观光旅游综合开发有限公司 | A kind of lily method for in-vitro rapid propagation |
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