CN104521527B - Rapid propagation method for hyacinths - Google Patents

Rapid propagation method for hyacinths Download PDF

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Publication number
CN104521527B
CN104521527B CN201510004362.2A CN201510004362A CN104521527B CN 104521527 B CN104521527 B CN 104521527B CN 201510004362 A CN201510004362 A CN 201510004362A CN 104521527 B CN104521527 B CN 104521527B
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ball
seed
balls
mother
mother bulb
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CN104521527A (en
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贾文杰
王祥宁
崔光芬
吴丽芳
蒋亚莲
段青
马璐琳
杜文文
王继华
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Flower Research Institute of YAAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention provides a rapid propagation method for hyacinths. The method is characterized by comprising the steps that mother balls with developed and robust seed-base root systems are selected out, with the seed-base as a plane, the mother balls are rotatably cut from the inner side of the seed-base by inclining the seed-base by 30 degrees, sulphur powder with the sulphur mass content being 1 % is evenly smeared on cuts of the mother balls, and the cuts are made upward to be aired by 2-3 hours under the sun at the high noon; the mother balls are propagated in a dark culture mode in a well-ventilated culture room with the temperature being 25 DEG C and the humidity being lower than 50 % until small seed balls grow on the mother balls, the small seed balls are stripped off, buried in a matrix and stored for 80-90 days under 25 DEG C for summer dormancy, the small seed balls are taken out, planted in sandy soil good in drainage and managed according to conventions, and seed balls are dug out until June to July of the second year; the seed balls are stored for 80-90 days under 25 DEG C, then planted and dug out until June to July of the third year, and therefore flowered hyacinth seed balls are obtained. The propagation efficiency of the hyacinths is high, and 8-10 small seed balls with the uniform size can be averagely propagated from each mother ball.

Description

A kind of hyacinthine method for quickly breeding
Technical field
The present invention relates to a kind of propagation method, a kind of hyacinthine method for quickly breeding, belong to technical field of plant culture.
Background technology
Hyacinth is draft napiform root class plant for many years, and bulb is avette, has film quality crust, epithelium color to become positive correlation with pattern.Shape such as Bulbus Allii when not blooming, originates in Mediterranean and Asia Minor one band, is a kind the most fragrant in the plant that can bloom at present.In recent years, along with China's expanding economy and the raising of living standards of the people, hyacinth has become high-grade pot flowers important in China flowers market, and is widely used in the fields such as courtyard greening, home decoration, flower in red-letter day.Along with being continuously increased of hyacinth demand, conventional method for tissue culture is because of the more high reason of inefficient, complex procedures, material and cost of labor, and cannot meet the Production requirement of extensive hyacinth kind ball;Additionally conventional scale cuttage breeding method, although workload is relatively fewer, and breeding coefficient is the highest, but easily infected by pest and disease damage and cause the problem planting the ball underproduction in a large number, bring unnecessary loss to enterprise.It is therefore desirable to prior art is improved.
Summary of the invention
For the existing hyacinth reproduction technique of solution, inefficient, propagating materials and cost of labor are higher; production routine is relative complex; it is difficult to large-scale production; Technique on Cuttage Propagation is easily by problems such as pest and disease damage infection, and the present invention provides a kind of time and labour saving, the rate of increase is high, seed ball growth quality is good, diseases and insect pests resistance is strong, can preserve mother bulb merit, meet the hyacinth method for quickly breeding of the large-scale production needs of high quality seedling.
The present invention is to provide the hyacinthine method for quickly breeding of such a, it is characterised in that comprise the following steps:
A. annual March~April basal disc well developed root system, healthy and strong mother bulb are selected, with basal disc as plane, tilting 30 degree of circumgyration incision mother bulbs inside basal disc, depth of cut is 1.4~1.6cm, a diameter of 4.0~6.0cm, and with the alcohol wipe knife face that mass concentration is 95% every time after cutting;
B. with the Cosan uniform application that sulfur mass content is 1% on the mother bulb otch of step A, and make otch upwards, air-dry 2~3 hours under solar noon sun;
C. the mother bulb of step B is divided in 25 DEG C, relative humidity less than 50%, in breezy culturing room, continuous light culture is bred 70~75 days, until growing seed ball on mother bulb;Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. strip seeds ball from the mother bulb of step C, obtains mitogenetic seed ball, this mitogenetic seed bag is embedded in biodiversity content be 35~40% substrate in, and with gauze bag wrap up, at 25 DEG C storage within 80~90 days, carry out aestivation;Containing the carbendazim antibacterial that mass ratio is 1% in described substrate;
E. 10 then~November, seed ball step D processed through aestivation, is planted in well-drained sand, and planting environment requires that illumination, ventilation condition are good, plant ball transplanting according to routine at letter, implantation methods is managed, until Second Year June~July dig out kind of a ball;
F. after kind ball step E dug out is stored 80~90 days in 25 DEG C of environment, in Second Year 10~November, the kind ball stored is planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, transplant according to conventional hyacinth kind ball, implantation methods is managed, until in June, the 3rd~July dig out kind of a ball, i.e. obtain hyacinth kind ball of blooming.
The present invention has following advantages and an effect:
1. breed hyacinth seed ball by the present invention, whole year production can be realized in Nostoc commune Vanch indoor, both saved land resource, and improve again economic benefit.
2. reproductive efficiency is high, cultivates with conventional organization and compared with cottage propagation, substantially increases hyacinthine reproductive efficiency, and average each mother bulb can breed seed ball 8 of uniform size~10, and reproductive efficiency is greatly improved.
3. save substantial amounts of manpower and materials, special patterning method only needs manually to cut hyacinth mother bulb once, just can breed hyacinth seed ball 8~10, and method is easy, and use Conventional solid culture medium culturing, owing to callus is grown thickly, transfer more difficult, and number of times is various, generally it is linked into seed ball bottle outlet from outer implant, needing human relay working 4~5 times altogether, switching need to reconfigure solid medium every time, thus wastes substantial amounts of human and material resources.
4. solve the seedling parental source complexity that conventional breeding of method produces, the difficult problem that seed ball quality is unstable, pass through the present invention, make seed ball character stable, seed ball source is single, it is prone to standardization, batch production operation, is effectively improved seed ball quality, can be that market provides the excellent hyacinth kind ball sought unity of standard.
5. seed ball field cultivation survival rate is higher.Owing to this method seed ball flourish environment is closer to natural environment, it is not necessary to grow in the culture bottle of high humility, plant ball transplanting survival rate close to 100%.
6. the present invention is in terms of the selection of mother bulb, and optional fancy points is good, and the stronger hyacinth kind ball of disease-resistant, insect resistace is as breeding mother bulb.The kind by the method for quickly breeding of the present invention, fancy points is good, disease-resistant, insect resistace is strong is fixing rapidly and remains behind, and is effectively shortened breeding cycle.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1
A. basal disc well developed root system, healthy and strong mother bulb, with lunar shape knife, with basal disc as plane, oblique 30 degree of circumgyration incision mother bulbs inside basal disc, degree of depth 1.5cm, diameter 5.0cm are selected annual March;With 95% alcohol wipe knife face every time after cutting;
B. with the Cosan uniform application that sulfur mass content is 1% on the mother bulb otch of step A, afterwards by otch upwards, air-dry 3 hours under solar noon sun;
C. the mother bulb of step B is divided in 25 DEG C, relative humidity less than 50%, in the preferable culturing room of ventilated environment, continuous light culture is bred 72 days, until growing seed ball on mother bulb;Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. strip seeds ball from the mother bulb of step C, obtains mitogenetic seed ball, is embedded in the substrate that biodiversity content is 37% by this mitogenetic seed bag, and wraps up with gauze bag, and at 25 DEG C, storage carries out aestivation in 85 days;Containing the carbendazim antibacterial that mass ratio is 1% in described substrate;
E. planting October then in well-drained sand by the seed ball processed through the aestivation of step D, planting environment requires that illumination, ventilation condition are good, is managed, until the June of Second Year digs out kind of a ball according to conventional hyacinth kind ball transplanting, implantation methods;
F. kind ball step E dug out was placed in 25 DEG C of environment storage after 85 days, seed ball is planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, transplant according to conventional hyacinth kind ball, implantation methods is managed, until digging out kind of a ball June of the 3rd year, obtain hyacinth kind ball of blooming.
Average each mother bulb can breed 8, seed ball of uniform size, and reproductive efficiency is greatly improved, and keeps the feature that mother bulb is healthy and strong, fancy points performance good and disease-resistant, pest-resistant is strong, survival rate 99% after transplanting.
Embodiment 2
Hyacinthine method for quickly breeding, it is characterised in that comprise the following steps:
A. selecting basal disc well developed root system, healthy and strong mother bulb annual April, with basal disc as plane, tilt 30 degree of circumgyration incision mother bulbs inside basal disc, depth of cut is 1.4cm, a diameter of 6.0cm, and with the alcohol wipe knife face that mass concentration is 95% every time after cutting;
B. with the Cosan uniform application that sulfur mass content is 1% on the mother bulb otch of step A, and make otch upwards, air-dry 2 hours under solar noon sun;
C. the mother bulb of step B is divided in 25 DEG C, relative humidity less than 50%, in breezy culturing room, continuous light culture is bred 70 days, until growing seed ball on mother bulb;Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. strip seeds ball from the mother bulb of step C, obtains mitogenetic seed ball, is embedded in the substrate that biodiversity content is 35% by this mitogenetic seed bag, and wraps up with gauze bag, and at 25 DEG C, storage carries out aestivation in 80 days;Containing the carbendazim antibacterial that mass ratio is 1% in described substrate;
E. November then, seed ball step D processed through aestivation, is planted in well-drained sand, and planting environment requires that illumination, ventilation condition are good, plant ball transplanting according to routine at letter, implantation methods is managed, until Second Year digs out kind of a ball July;
F. after kind ball step E dug out is stored 80 days in 25 DEG C of environment, November the kind ball stored is planted in well-drained sand in Second Year, planting environment requires that illumination, ventilation condition are good, transplant according to conventional hyacinth kind ball, implantation methods is managed, until in June, the 3rd digs out kind of a ball, i.e. obtain hyacinth kind ball of blooming.
Average each mother bulb can breed 10, seed ball of uniform size, and reproductive efficiency is greatly improved, and keeps the feature that mother bulb is healthy and strong, fancy points performance good and disease-resistant, pest-resistant is strong, survival rate 99.5% after transplanting.
Embodiment 3
Hyacinthine method for quickly breeding, it is characterised in that comprise the following steps:
A. selecting basal disc well developed root system, healthy and strong mother bulb annual March, with basal disc as plane, tilt 30 degree of circumgyration incision mother bulbs inside basal disc, depth of cut is 1.6cm, a diameter of 6.0cm, and with the alcohol wipe knife face that mass concentration is 95% every time after cutting;
B. with the Cosan uniform application that sulfur mass content is 1% on the mother bulb otch of step A, and make otch upwards, air-dry 3 hours under solar noon sun;
C. the mother bulb of step B is divided in 25 DEG C, relative humidity less than 50%, in breezy culturing room, continuous light culture is bred 75 days, until growing seed ball on mother bulb;Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. strip seeds ball from the mother bulb of step C, obtains mitogenetic seed ball, is embedded in the substrate that biodiversity content is 40% by this mitogenetic seed bag, and wraps up with gauze bag, and at 25 DEG C, storage carries out aestivation in 90 days;Containing the carbendazim antibacterial that mass ratio is 1% in described substrate;
E. October then, seed ball step D processed through aestivation, is planted in well-drained sand, and planting environment requires that illumination, ventilation condition are good, plant ball transplanting according to routine at letter, implantation methods is managed, until Second Year digs out kind of a ball July;
F. after kind ball step E dug out is stored 90 days in 25 DEG C of environment, November the kind ball stored is planted in well-drained sand in Second Year, planting environment requires that illumination, ventilation condition are good, transplant according to conventional hyacinth kind ball, implantation methods is managed, until in July, the 3rd digs out kind of a ball, i.e. obtain hyacinth kind ball of blooming.
Average each mother bulb can breed 9, seed ball of uniform size, and reproductive efficiency is greatly improved, and keeps the feature that mother bulb is healthy and strong, fancy points performance good and disease-resistant, pest-resistant is strong, survival rate 99.8% after transplanting.

Claims (1)

1. a hyacinthine method for quickly breeding, it is characterised in that comprise the following steps:
A. annual March~April basal disc well developed root system, healthy and strong mother bulb are selected, with basal disc as plane, tilting 30 degree of circumgyration incision mother bulbs inside basal disc, depth of cut is 1.4~1.6cm, a diameter of 4.0~6.0cm, and with the alcohol wipe knife face that mass concentration is 95% every time after cutting;
B. with the Cosan uniform application that sulfur mass content is 1% on the mother bulb otch of step A, and make otch upwards, air-dry 2~3 hours under solar noon sun;
C. the mother bulb of step B is divided in 25 DEG C, relative humidity less than 50%, in breezy culturing room, continuous light culture is bred 70~75 days, until growing seed ball on mother bulb;Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. strip seeds ball from the mother bulb of step C, obtains mitogenetic seed ball, this mitogenetic seed bag is embedded in biodiversity content be 35~40% substrate in, and with gauze bag wrap up, at 25 DEG C storage within 80~90 days, carry out aestivation;Containing the carbendazim antibacterial that mass percent is 1% in described substrate;
E. 10 then~November, seed ball step D processed through aestivation, is planted in well-drained sand, and planting environment requires that illumination, ventilation condition are good, transplant at hyacinth kind ball according to routine, implantation methods is managed, until Second Year June~July dig out kind of a ball;
F. after kind ball step E dug out is stored 80~90 days in 25 DEG C of environment, in Second Year 10~November, the kind ball stored is planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, transplant according to conventional hyacinth kind ball, implantation methods is managed, until in June, the 3rd~July dig out kind of a ball, i.e. obtain hyacinth kind ball of blooming.
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CN106212009A (en) * 2016-08-01 2016-12-14 上海交通大学 The borehole propagation method of Narcissus Tazetta L. Var
CN107173195A (en) * 2017-06-08 2017-09-19 合肥慧明瀚生态农业科技有限公司 A kind of hyacinthine ciltivating process

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CN103891515A (en) * 2014-04-15 2014-07-02 李蓁 Planting method for common grape hyacinth flowers

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CN101242739A (en) * 2005-06-29 2008-08-13 先正达参股股份有限公司 Liquid compositions for treating plant propagation materials
CN102511391A (en) * 2011-12-07 2012-06-27 上海交通大学 Hyacinthus orientalis L. in-vitro rapid propagation method
CN103168580A (en) * 2011-12-26 2013-06-26 江苏美尚生态景观股份有限公司 Hormone treatment method for hyacinth cutting scales
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