CN106212009A - The borehole propagation method of Narcissus Tazetta L. Var - Google Patents

The borehole propagation method of Narcissus Tazetta L. Var Download PDF

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Publication number
CN106212009A
CN106212009A CN201610620806.XA CN201610620806A CN106212009A CN 106212009 A CN106212009 A CN 106212009A CN 201610620806 A CN201610620806 A CN 201610620806A CN 106212009 A CN106212009 A CN 106212009A
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China
Prior art keywords
bulb
narcissus
var
borehole
propagation method
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CN201610620806.XA
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Chinese (zh)
Inventor
李欣
史益敏
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention provides the borehole propagation method of a kind of Narcissus Tazetta L. Var;Described method comprises the steps: step 1, the bulb that water intaking is celestial, is hollowed out on the bulb chassis of narcissus, and the plateau edge retaining narcissus is intact;Step 2, processes with antibacterial;Step 3, is laid in Medium Culture by the bulb of described narcissus, preserves in warm, dry, dark surrounds, forms callus;Step 4, is stored in and is dried cold preservation in dark warehouse, until plantation.The borehole reproduction technique of the present invention, simple, safe and efficient, i.e. improve the breeding coefficient of Narcissus Tazetta L. Var, the most do not affect the growth of mother bulb self this season;And during planting, be beneficial to Nutrient Absorption and the growth of newborn bead, thus shorten bulb and bloom the required time.

Description

The borehole propagation method of Narcissus Tazetta L. Var
Technical field
The present invention relates to flowers propagation method field;A kind of borehole propagation method of Narcissus Tazetta L. Var.
Background technology
Narcissus (Narcissus pseudonarcissusL.) is the perennial ornamental herb of Amaryllidaceae Narcissus, its Pattern is gorgeous, and flower pattern is peculiar, is widely used in afforestation.The propagation method of narcissus mainly has seminal propagation, bulb separation breeding And tissue culture.Cycle needed for seminal propagation is long, is easily generated bud mutation;Under natural conditions, the bulb that bulb separation breeding produces is little, number Amount is few, easy infection disease;Tissue culture is more efficient, but operation complexity, technology content is higher, relatively costly.Therefore, letter is sought Just, efficiently, quick propagation method is most important.
The most report the bulb reproduction technique of multiple flowering bulb, as patent 200910095164.6 describes directly Stripping the technology of scale lining lily ball, patent 201010537560.3 describes cuttage propagation method for bulb scale in tulip, specially The method that profit 201010537667.7 describes bigbloom onion cottage propagation.But above-mentioned several flowering bulb asexual reproduction method is the most broken It is broken the growth of mother bulb, affects blooming of mother bulb this season, and the bulbec week path quality of isolated is the least, becomes bulb of blooming The required cycle is longer.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide the borehole propagation method of a kind of Narcissus Tazetta L. Var.
The present invention is achieved by the following technical solutions:
The present invention provides the borehole propagation method of a kind of Narcissus Tazetta L. Var, and described method comprises the steps:
Step 1, the bulb that water intaking is celestial, the bulb chassis of narcissus is hollowed out, the plateau edge retaining narcissus is intact;
Step 2, processes with antibacterial;
Step 3, is laid in Medium Culture by the bulb of described narcissus, preserves in warm, dry, dark surrounds, is formed more Injured tissue;
Step 4, is stored in and is dried cold preservation in dark warehouse, until plantation.
Preferably, in step 1, the bulb of described narcissus is that 3-5 is raw, in week footpath 12-18cm, encroaches on without pathogenic bacteria, and basal disc is complete Whole, without rotting.
Preferably, in step 1, the degree of depth of described borehole is 1-2.5cm.
Preferably, in step 2, described antibacterial is carbendazim, and extension rate is 600-800 times.
Preferably, in step 2, described antibacterial processes method particularly includes: soak the bulb 5-of narcissus with described antibacterial 10min, pulls control water out and dries in the shade.
Preferably, in step 3, described substrate is that plant ash, river sand mix for 5-10:2-5 in mass ratio.
Preferably, in step 3, the condition of described bulb storage is temperature 20-25 DEG C, and humidity is 30-45%.
Preferably, in step 3, the time of described formation callus is 6-8 days.
Preferably, in step 3, bulb storage cuts off the time and avoids bulb to Medium Culture water spray to improve humidity Desiccation.
Preferably, in step 4, the condition of described cold preservation is 4-6 DEG C of Cold storage in the refrigerator, and the time is 8-10 week.
Compared with prior art, the present invention has a following beneficial effect:
1. the present invention uses borehole reproduction technique, simple, safe and efficient, can increase the line of breeding of narcissus bead Number, promotes it to germinate bead more;
2. the inventive method does not interferes with mother bulb this season and blooms, and the bulbec quality that the coming year separates from mother bulb is relatively big, from And shorten the time become required for bulb of blooming.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in the technology of this area Personnel are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, the ordinary skill to this area For personnel, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into the present invention Protection domain.
Embodiment 1
The present embodiment relates to the borehole propagation method of a kind of Narcissus Tazetta L. Var, and described method comprises the steps:
Step 1, using agriculture butterfly shadow (N.pseudonarcissus ' Shangnongdieying ') on Varieties of Narcissus Tazetta Var is examination Material, on August 5th, 2012, chooses life in 3 years, and all footpaths 12-14cm Maturity is high, and without pest and disease damage, the bulb that basal disc is complete, with point Bulb chassis is hollowed out by sharp soupspoon or cochlear cutter, the degree of depth about 1.5cm, retains plateau edge intact;
Step 2, after soaking 5min with 600 times of carbendazim, pulls control water out and dries in the shade;
Step 3, the bulb after processing is laid in plant ash: river sand mixes Medium Culture, Yu Wen with the volume ratio of 5:2 Spend 20 DEG C, in humidity 30% dark surrounds storage place;Callus is formed, when cutting off in bulb storage after 6-8 days Between to Medium Culture water spray with improve humidity avoid bulb desiccation;
Step 4, is stored in 5 DEG C on the 20th in JIUYUE and is dried cold preservation 8 weeks in dark warehouse, and November 15 planted;Plantation condition is Planting Row Distance 20cm, spacing in the rows is about 3 times of kind bulb diameters, 1 times of thickness of earth covering substantially bulb height.
Implementation result: adding up after receiving ball on June 10th, 2013 and show, the breeding coefficient of upper agriculture butterfly shadow reaches 6.35, and borehole is numerous The bulbec quantity grown is higher 3.5 times than the bulbec quantity of bulb separation breeding under natural conditions.
Embodiment 2
The present embodiment relates to the borehole propagation method of a kind of Narcissus Tazetta L. Var, and described method comprises the steps:
Step 1, using agriculture early spring (N.pseudonarcissus ' Shangnongzaochun ') on Varieties of Narcissus Tazetta Var is examination Material, chose life in 5 years on August 5th, 2012, and all footpaths 14cm Maturity is high, without pest and disease damage, and the bulb that basal disc is complete;Use sharp-pointed soup Bulb chassis is hollowed out by spoon or cochlear cutter, the degree of depth about 1.2cm, retains plateau edge intact;
Step 2, after soaking 8min with 700 times of carbendazim, pulls control water out and dries in the shade;
Step 3, the bulb after processing is laid in plant ash: river sand mixes Medium Culture, Yu Wen with the volume ratio of 8:3 Spend 20 DEG C, in humidity 30% dark surrounds storage place;Callus is formed, when cutting off in bulb storage after 6-8 days Between to Medium Culture water spray with improve humidity avoid bulb desiccation;
Step 4, is stored in 5 DEG C on the 20th in JIUYUE and is dried cold preservation 8 weeks in dark warehouse, and November 15 planted;Plantation condition is Planting Row Distance 20cm, spacing in the rows is about 3 times of kind bulb diameters, 1 times of thickness of earth covering substantially bulb height.
Implementation result: receive on June 10th, 2013 to add up after ball and show, the breeding coefficient in upper agriculture early spring reaches 5.20, and borehole is numerous The bulbec quantity grown is higher 3 times than the bulbec quantity of bulb separation breeding under natural conditions.
Embodiment 3
The present embodiment relates to the borehole propagation method of a kind of Narcissus Tazetta L. Var, and described method comprises the steps:
Step 1, using the red shadow of agriculture (N.pseudonarcissus ' Shangnonghongying ') on Varieties of Narcissus Tazetta Var is examination Material, chose life in 5 years on August 5th, 2012, and all footpaths 13cm Maturity is high, without pest and disease damage, and the bulb that basal disc is complete;
Step 2, hollows out bulb chassis with sharp-pointed soupspoon or cochlear cutter, the degree of depth about 1.5cm, retains plateau edge complete Good;After soaking 10min with 700 times of carbendazim, pull control water out and dry in the shade;
Step 3, the bulb after processing is laid in plant ash: river sand mixes Medium Culture with the volume ratio of 10:5, in In temperature 20 DEG C, humidity 30% dark surrounds, storage is placed;Form callus after 6-8 days, cut off in bulb storage Time avoids bulb desiccation to Medium Culture water spray to improve humidity;
Step 4, is stored in 5 DEG C on the 20th in JIUYUE and is dried cold preservation 8 weeks in dark warehouse, and November 15 planted;Plantation condition is Planting Row Distance 20cm, spacing in the rows is about 3 times of kind bulb diameters, 1 times of thickness of earth covering substantially bulb height.
Implementation result: adding up after receiving ball on June 10th, 2013 and show, the breeding coefficient of the red shadow of upper agriculture reaches 5.17, and borehole is numerous The bulbec quantity grown is higher 3 times than the bulbec quantity of bulb separation breeding under natural conditions.
Embodiment 4
The present embodiment relates to the borehole propagation method of a kind of Narcissus Tazetta L. Var, and described method comprises the steps:
Step 1, uses Varieties of Narcissus Tazetta Var De Labo (N.pseudonarcissus ' Delibes ') for trying material, in 2012 8 Within 5th, choosing life in 3 years the moon, all footpaths 12-14cm Maturity is high, without pest and disease damage, and the bulb that basal disc is complete;With sharp-pointed soupspoon or cochlear cutter Bulb chassis is hollowed out, the degree of depth about 1.2cm, retain plateau edge intact;
Step 2, after soaking 10min with 800 times of carbendazim, pulls control water out and dries in the shade;
Step 3, the bulb after processing is laid in plant ash: river sand mixes Medium Culture, Yu Wen with the volume ratio of 7:3 Spend 20 DEG C, in humidity 30% dark surrounds storage place;Callus is formed, when cutting off in bulb storage after 6-8 days Between to Medium Culture water spray with improve humidity avoid bulb desiccation;
Step 4, is stored in 5 DEG C on the 20th in JIUYUE and is dried cold preservation 8 weeks in dark warehouse, and November 15 planted;Plantation condition is Planting Row Distance 20cm, spacing in the rows is about 3 times of kind bulb diameters, 1 times of thickness of earth covering substantially bulb height.
Implementation result: adding up after receiving ball on June 10th, 2013 and show, the breeding coefficient of De Labo reaches 4.83, and borehole is bred Bulbec quantity than under natural conditions bulb separation breeding bulbec quantity high 2.5 times.
Embodiment 5
The present embodiment relates to the borehole propagation method of a kind of Narcissus Tazetta L. Var, and described method comprises the steps:
Step 1, using agriculture butterfly shadow (N.pseudonarcissus ' Shangnongdieying ') on Varieties of Narcissus Tazetta Var is examination Material, on August 5th, 2012, chooses life in 3 years, and all footpaths 12-14cm Maturity is high, and without pest and disease damage, the bulb that basal disc is complete, with point Bulb chassis is hollowed out by sharp soupspoon or cochlear cutter, the degree of depth about 2.5cm, retains plateau edge intact;
Step 2, after soaking 5min with 600 times of carbendazim, pulls control water out and dries in the shade;
Step 3, the bulb after processing is laid in plant ash: river sand mixes Medium Culture, Yu Wen with the volume ratio of 5:2 Spend 20 DEG C, in humidity 30% dark surrounds storage place;Callus is formed, when cutting off in bulb storage after 6-8 days Between to Medium Culture water spray with improve humidity avoid bulb desiccation;
Step 4, is stored in 5 DEG C on the 20th in JIUYUE and is dried cold preservation 8 weeks in dark warehouse, and November 15 planted;Plantation condition is Planting Row Distance 20cm, spacing in the rows is about 3 times of kind bulb diameters, 1 times of thickness of earth covering substantially bulb height.
Implementation result: adding up after receiving ball on June 10th, 2013 and show, the breeding coefficient of upper agriculture butterfly shadow reaches 5.35, and borehole is numerous The bulbec quantity grown is higher 2.8 times than the bulbec quantity of bulb separation breeding under natural conditions.
Embodiment 6
The present embodiment relates to the borehole propagation method of a kind of Narcissus Tazetta L. Var, and described method comprises the steps:
Step 1, using agriculture butterfly shadow (N.pseudonarcissus ' Shangnongdieying ') on Varieties of Narcissus Tazetta Var is examination Material, on August 5th, 2012, chooses life in 3 years, and all footpaths 12-14cm Maturity is high, and without pest and disease damage, the bulb that basal disc is complete, with point Bulb chassis is hollowed out by sharp soupspoon or cochlear cutter, the degree of depth about 1cm, retains plateau edge intact;
Step 2, after soaking 5min with 600 times of carbendazim, pulls control water out and dries in the shade;
Step 3, the bulb after processing is laid in plant ash: river sand mixes Medium Culture, Yu Wen with the volume ratio of 6:2 Spend 20 DEG C, in humidity 30% dark surrounds storage place;Callus is formed, when cutting off in bulb storage after 6-8 days Between to Medium Culture water spray with improve humidity avoid bulb desiccation;
Step 4, is stored in 5 DEG C on the 20th in JIUYUE and is dried cold preservation 8 weeks in dark warehouse, and November 15 planted;Plantation condition is Planting Row Distance 20cm, spacing in the rows is about 3 times of kind bulb diameters, 1 times of thickness of earth covering substantially bulb height.
Implementation result: adding up after receiving ball on June 10th, 2013 and show, the breeding coefficient of upper agriculture butterfly shadow reaches 6.05, and borehole is numerous The bulbec quantity grown is higher 3.0 times than the bulbec quantity of bulb separation breeding under natural conditions.
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned Particular implementation, those skilled in the art can make various deformation or amendment within the scope of the claims, this not shadow Ring the flesh and blood of the present invention.

Claims (9)

1. the borehole propagation method of a Narcissus Tazetta L. Var, it is characterised in that described method comprises the steps:
Step 1, the bulb that water intaking is celestial, the bulb chassis of narcissus is hollowed out, the plateau edge retaining narcissus is intact;
Step 2, processes with antibacterial;
Step 3, is laid in Medium Culture by the bulb of described narcissus, storage, forms callus;
Step 4, is stored in and is dried cold preservation in dark warehouse, until plantation.
2. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 1, the squama of described narcissus Stem is that 3-5 is raw, in week footpath 12-18cm, encroaches on without pathogenic bacteria, and basal disc is complete, without rotting.
3. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 1, described borehole deep Degree is 1-2.5cm.
4. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 2, described antibacterial is Carbendazim, extension rate is 600-800 times.
5. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 2, at described antibacterial Reason method particularly includes: soak the bulb 5-10min of narcissus with described antibacterial, pulls control water out and dries in the shade.
6. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 3, described substrate is grass Wood ash, river sand mix for 5-10:2-5 in mass ratio.
7. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 3, described bulb is preserved Condition be temperature 20-25 DEG C, humidity is 30-45%.
8. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 3, described formation wound healing The time of tissue is 6-8 days.
9. the borehole propagation method of Narcissus Tazetta L. Var as claimed in claim 1, it is characterised in that in step 4, the bar of described cold preservation Part is 4-6 DEG C of Cold storage in the refrigerator, and the time is 8-10 week.
CN201610620806.XA 2016-08-01 2016-08-01 The borehole propagation method of Narcissus Tazetta L. Var Pending CN106212009A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107318408A (en) * 2017-06-19 2017-11-07 莆田学院 A kind of Do.Dick wilden daffodil propagation methods
CN108770512A (en) * 2018-03-23 2018-11-09 福建农林大学 The method of annual narcissus breeding
CN110249743A (en) * 2019-07-18 2019-09-20 四川迪菲特药业有限公司 A kind of processing method of watt of cloth fritillaria kind bulb
CN115843692A (en) * 2022-12-29 2023-03-28 浙江省农业科学院 Allium fistulosum tissue culture method

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CN103444395A (en) * 2013-08-16 2013-12-18 苏州仁成生物科技有限公司 Pollution-free cultivation production method for young narcissus balls
CN104429577A (en) * 2014-12-31 2015-03-25 福建农林大学 Simple paper-white narcissus propagation method
CN104521527A (en) * 2015-01-06 2015-04-22 云南省农业科学院花卉研究所 Rapid propagation method for hyacinths
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Publication number Priority date Publication date Assignee Title
CN103444395A (en) * 2013-08-16 2013-12-18 苏州仁成生物科技有限公司 Pollution-free cultivation production method for young narcissus balls
CN105474929A (en) * 2014-09-19 2016-04-13 青岛诚一知识产权服务有限公司 Cultivation method for Kalanchoe blossfeldiana
CN104429577A (en) * 2014-12-31 2015-03-25 福建农林大学 Simple paper-white narcissus propagation method
CN104521527A (en) * 2015-01-06 2015-04-22 云南省农业科学院花卉研究所 Rapid propagation method for hyacinths
CN105325092A (en) * 2015-11-03 2016-02-17 平潭综合实验区市政园林有限公司 Refrigeration and perpetual blooming nursing method for narcissi

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107318408A (en) * 2017-06-19 2017-11-07 莆田学院 A kind of Do.Dick wilden daffodil propagation methods
CN108770512A (en) * 2018-03-23 2018-11-09 福建农林大学 The method of annual narcissus breeding
CN110249743A (en) * 2019-07-18 2019-09-20 四川迪菲特药业有限公司 A kind of processing method of watt of cloth fritillaria kind bulb
CN115843692A (en) * 2022-12-29 2023-03-28 浙江省农业科学院 Allium fistulosum tissue culture method
CN115843692B (en) * 2022-12-29 2023-09-29 浙江省农业科学院 Tissue culture method for allium fistulosum

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Application publication date: 20161214