CN104663465A - Culture medium for heartleaf houttuynia herb tissue culture - Google Patents
Culture medium for heartleaf houttuynia herb tissue culture Download PDFInfo
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Abstract
The invention discloses a culture medium for heartleaf houttuynia herb tissue culture. The culture medium comprises a subculture medium and a rooting culture medium. Proliferation and rooting culture are performed by adding an appropriate amount of longan juice, so that the growth of an adventitious bud of heartleaf houttuynia herb can be promoted, and the proliferation coefficient is high; and furthermore, the rooting is simultaneously induced during proliferation, and the culture period is shortened. The rooting rate and the transplantation survival rate are high, and the genetic stability is good.
Description
Technical field
The present invention relates to tissue culture medium (TCM), particularly relate to a kind of cordate houttuynia culture medium for tissue culture.
Background technology
Cordate houttuynia, English name HERBA HOUTTUYNIAE is the herbal medicine that Chinese Pharmacopoeia is included, and herbal medicine source is the dry aerial parts of saururaceae plant houttuynia cordata (Classification system: Houttuynia cordata Thunb.).Tap when the luxuriant colored fringe of cauline leaf in summer is many, removing impurity, dries.Herbal medicine proterties: stem is oblate cylindricality, distortion, long 20-35cm, diameter 0.2-0.3cm; Surface brown color, tool indulge rib several, joint obviously, bottom joint has remaining fibrous root; Matter is crisp, frangibility.Leaf alternate, the shrinkage of blade convolution, in heart-shaped after flattening, long 3-5cm, wide 3-4.5cm; Tip is gradually sharp, Quan Yuan; Upper surface darker yellow green to burgundy, lower surface celadon or taupe brown; Petiole is elongated, and base portion becomes constitute sheath-like with stipule symphysis.Spike top is raw, yellowish-brown.Rub the broken taste that bears the odor of fish with the hands.Cordate houttuynia taste is pungent, cold, returns lung channel.Clearing heat and detoxicating, detumescence can treat sore, promoting urination and removing dampness, clearing away heat to cure dysentery, stomach strengthening and digestion promoting, by the lung carbuncle, sore swollen toxin, hemorrhoidal hemorrhage, retention of heat in the spleen and stomach etc. of controlling real heat, heat poison, damp evil, disease heat are trouble.Modern pharmacology experiment shows, this product has antibacterial, antiviral, effect such as raising immunity of organisms, diuresis etc.
Cordate houttuynia China Yangtze river basin and on the south each province all have wild distribution.Cordate houttuynia is perennial root herbaceous plant, and property happiness warm and moist environment, can grow in various soil, grow the most vigorous with loose fertile neutrality or subacidity sandy soil (or sandy loam), be afraid of frost, not drought-resistant and waterlogging, shade tolerance is strong.Cordate houttuynia has very high medical value and edibility, and its plant strain growth is healthy and strong, resistant to diseases and insects is extremely strong, generally do not need to carry out diseases and pests controlling in production cultivation, can from drug contamination, be a kind of free of contamination medicine, dish dual-purpose plant, have vast potential for future development as green non-pollution health vegetable development and utilization.Due to excessive collection, current heartleaf houttuynia wild resource destroys serious, in order to protect wild resource, answers active development utilize and carry out artificial family introducing and planting.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of cordate houttuynia culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of often liter of (L) subculture medium and the content of each component as follows:
(1) macroelement: potassium nitrate 1800mg/L, ammonium nitrate 1350mg/L, calcium chloride dihydrate 350mg/L, epsom salt 320mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassium iodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, vitamin b3 (VB
2) 6.0mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, thiamine hydrochloride (VB
1) 0.1mg/L;
(4) plant growth regulator: 6-benzyladenine 0.5-2.0mg/L, methyl α-naphthyl acetate 0.2-0.8mg/L, longan juice 0.3-0.5mg/L;
Surplus is distilled water;
The component of often liter of (L) root media and the content of each component as follows:
(1) macroelement: potassium nitrate 900mg/L, ammonium nitrate 675mg/L, calcium chloride dihydrate 175mg/L, epsom salt 160mg/L, potassium dihydrogen phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.3-0.6mg/L, longan juice 0.3-0.5mg/L;
Surplus is distilled water.
Longan juice of the present invention is that fresh longan removes branches and leaves, sick wormed fruit, decayed fruit, rotten fruit, crude fruit, and cleaning, removal pericarp fruit stone, pulp squeezes, and the fruit juice obtained, sterilizing obtains.
Subculture in cordate houttuynia culture medium for tissue culture of the present invention, the compound method of root media are:
The preparation of 1 mother liquor and preservation
1.1, for ease of sampling, should first prepare various mother liquor, be divided into macroelement mother liquor, micro-mother liquor, organic matter mother liquor and each plant growth regulators mother liquor.Sucrose, agar should not be made into mother liquor, directly sample when needing;
1.2 macroelement mother liquid concentrations become 100 times of solution, and organic matter, micro-mother liquor are made into 200 times of solution, and the concentration of plant-growing-help chemicals mother liquor is made into 1mg/ml;
1.3 mother liquors select aseptic distilled water, deionized water or ultra-pure water to prepare, with the water boiled during a large amount of production;
During 1.4 preparation macroelement mother liquor, each component should be dissolved separately, mixes one by one afterwards, otherwise easily cause precipitation by the order of nitrogen, calcium, magnesium, phosphorus;
1.5 trace elements, organic matter, plant growth regulator mother liquor application brown bottle install and are placed in refrigerator and preserve;
Should use in time after 1.6 mother liquor, period of storage was no more than 1 month;
1.7 find that mother liquor has precipitation, or have growth of microorganism, or have algal grown, should pass into disuse.
The preparation of 2 medium
2.1 according to culture medium prescription, measures various mother liquor in proportion;
2.2 put into the pure water that about more than 1/2 of medium total amount is prepared in preparation in dosing container, and add appropriate sugar, then add the mother liquor of aequum while stirring one by one;
2.3 agar can add after heat fused, also directly can add agar powder if any during mixing plant, then supply the total amount of required preparation medium with pure water, stir;
Sugar in 2.4 medium, in a large amount of plantlet in vitro is produced, general available commercially available white sugar, but preferably with sucrose, garden beet is not sugared;
2.5 regulate the pH value to 5.8 of medium with the hydrochloric acid of 1.0 mol/L or the sodium hydroxide of 1.0 mol/L;
2.6 medium prepared will be dispensed in culture vessel as early as possible, in order to avoid culture medium solidifying or retrogradation and be difficult to packing;
Should note during 2.7 packing medium avoiding medium to be bonded on bottleneck, if be stained with medium, bottleneck must be cleaned with clean gauze before lid bottle cap or bottle stopper.
3 medium sterilizations
3.1 sterilize dividing the medium installed to be loaded in high-pressure sterilizing pot;
At 3.2 heating initial stages, when the air pressure of sterilizing pan disinfection room reaches 0.05Mpa, open condensation trap, drain sterilization cold air inside;
3.3 when disinfection room internal gas pressure reach 0.11Mpa, temperature reach 121 DEG C time, start timing, keep temperature and pressure sterilization 15-20 minute;
3.4 close heating power supply switch, by slow exhaust mode discharge hot gas, open sterilization pot cover or door, take out medium when the air pressure of indoor to be sterilized is down to atmospheric pressure;
3.5 medium should be placed in the clean environment cooling of little air flowing and few dust, otherwise cause mycotic spore to enter culture vessel in cooling intake process, produce mould contamination.
4 medium storages
4.1 medium should be as far as possible now with the current;
4.2 can at the clean and immobilising environment short time storage medium of air, but the medium of storage more than 1 month should be passed into disuse.
The method of cordate houttuynia tissue cultures of the present invention is:
(1) the drawing materials and sterilization of explant: after the subterranean stem throttling water getting raw cordate houttuynia then cleans 4-6h, be cut into the stem section of band 1-2 stipes, with the mixed liquid dipping 12h of streptomycin and Benzylpenicillin sodium salt, the concentration of mixed liquor streptomycin and Benzylpenicillin sodium salt is 0.5g/L; Then 70% alcohol-pickled 30s is used; Again with 0.1% mercuric chloride sterilization 12min; After use aseptic water washing 4-5 time; Finally use 0.1% mercuric chloride sterilization lOmin, for subsequent use after aseptic water washing 5 times;
(2) Fiber differentiation: the stem section of the band 1-2 stipes disinfected is seeded in evoking adventive bud on MS+0.5mg/LNAA+2mg/L6-BA medium and produces, condition of culture is 25 DEG C ± 1 DEG C, intensity of illumination is 2000-30001x, light application time is obtain the high startup seedling of 1-3cm after 16h/d, 30d;
(3) Multiplying culture with take root: startup seedling is cut into single stem section, be seeded on subculture medium, condition of culture is 25 DEG C ± 1 DEG C, intensity of illumination is 2000-30001x, light application time is 16h/d, 5-8d, when budling grows to about 2.0cm, more healthy and stronger bud seedling is gone to root induction on root media by single cutting on Multiple Buds, and all the other bud seedlings go on subculture medium and proceed Multiplying culture.Culture of rootage needs at about 5-8d, and now the base portion of seedling forms white projection, and extends gradually, forms obvious young root, grows up to complete plantlet gradually, obtains the cordate houttuynia seedling of taking root that high 6-8cm is with 5-6 stipes;
(4) hardening and transplanting: the cordate houttuynia seedling that step (3) obtains is carried out acclimatization and transplants.
Advantage of the present invention is:
1, propagation and culture of rootage are by adding the longan juice of appropriate amount, and can promote the growth of cordate houttuynia indefinite bud, reproduction coefficient is high, and root induction while propagation, shorten cultivation period.
2, rooting rate and transplanting survival rate reach more than 99.5%, and genetic stability is good.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1:
Cordate houttuynia culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of often liter of (L) subculture medium and the content of each component as follows:
(1) macroelement: potassium nitrate 1800mg/L, ammonium nitrate 1350mg/L, calcium chloride dihydrate 350mg/L, epsom salt 320mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassium iodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, vitamin b3 (VB
2) 6.0mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, thiamine hydrochloride (VB
1) 0.1mg/L;
(4) plant growth regulator: 6-benzyladenine 0.5mg/L, methyl α-naphthyl acetate 0.2mg/L, longan juice 0.3mg/L;
Surplus is distilled water.
The component of often liter of (L) root media and the content of each component as follows:
(1) macroelement: potassium nitrate 900mg/L, ammonium nitrate 675mg/L, calcium chloride dihydrate 175mg/L, epsom salt 160mg/L, potassium dihydrogen phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.3mg/L, longan juice 0.4mg/L;
Surplus is distilled water.
Embodiment 2:
Cordate houttuynia culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of often liter of (L) subculture medium and the content of each component as follows:
(1) macroelement: potassium nitrate 1800mg/L, ammonium nitrate 1350mg/L, calcium chloride dihydrate 350mg/L, epsom salt 320mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassium iodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, vitamin b3 (VB
2) 6.0mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, thiamine hydrochloride (VB
1) 0.1mg/L;
(4) plant growth regulator: 6-benzyladenine 2.0mg/L, methyl α-naphthyl acetate 0.8mg/L, longan juice 0.5mg/L;
Surplus is distilled water.
The component of often liter of (L) root media and the content of each component as follows:
(1) macroelement: potassium nitrate 900mg/L, ammonium nitrate 675mg/L, calcium chloride dihydrate 175mg/L, epsom salt 160mg/L, potassium dihydrogen phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.6mg/L, longan juice 0.5mg/L;
Surplus is distilled water.
Embodiment 3:
Cordate houttuynia culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of often liter of (L) subculture medium and the content of each component as follows:
(1) macroelement: potassium nitrate 1800mg/L, ammonium nitrate 1350mg/L, calcium chloride dihydrate 350mg/L, epsom salt 320mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassium iodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, vitamin b3 (VB
2) 6.0mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, thiamine hydrochloride (VB
1) 0.1mg/L;
(4) plant growth regulator: 6-benzyladenine 0.5mg/L, methyl α-naphthyl acetate 0.2mg/L, longan juice 0.3mg/L;
Surplus is distilled water.
The component of often liter of (L) root media and the content of each component as follows:
(1) macroelement: potassium nitrate 900mg/L, ammonium nitrate 675mg/L, calcium chloride dihydrate 175mg/L, epsom salt 160mg/L, potassium dihydrogen phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.3mg/L, longan juice 0.3mg/L;
Surplus is distilled water.
Embodiment 4:
Cordate houttuynia culture medium for tissue culture, this medium comprises subculture medium and root media, wherein:
The component of often liter of (L) subculture medium and the content of each component as follows:
(1) macroelement: potassium nitrate 1800mg/L, ammonium nitrate 1350mg/L, calcium chloride dihydrate 350mg/L, epsom salt 320mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassium iodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, vitamin b3 (VB
2) VB
26.0mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, thiamine hydrochloride (VB
1) 0.1mg/L;
(4) plant growth regulator: 6-benzyladenine 2.0mg/L, methyl α-naphthyl acetate 0.8mg/L, longan juice 0.4mg/L;
Surplus is distilled water.
The component of often liter of (L) root media and the content of each component as follows:
(1) macroelement: potassium nitrate 900mg/L, ammonium nitrate 675mg/L, calcium chloride dihydrate 175mg/L, epsom salt 160mg/L, potassium dihydrogen phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.6mg/L, longan juice 0.4mg/L;
Surplus is distilled water.
Comparative example:
(1) the drawing materials and sterilization of explant: after the subterranean stem throttling water getting raw cordate houttuynia then cleans 4-6h, be cut into the stem section of band 1-2 stipes, with the mixed liquid dipping 12h of streptomycin and Benzylpenicillin sodium salt, the concentration of mixed liquor streptomycin and Benzylpenicillin sodium salt is 0.5g/L; Then 70% alcohol-pickled 30s is used; Again with 0.1% mercuric chloride sterilization 12min; After use aseptic water washing 4-5 time; Finally use 0.1% mercuric chloride sterilization lOmin, for subsequent use after aseptic water washing 5 times;
(2) Fiber differentiation: the stem section of the band 1-2 stipes disinfected is seeded in evoking adventive bud on MS+0.5mg/LNAA+2mg/L6-BA medium and produces, condition of culture is 25 DEG C ± 1 DEG C, intensity of illumination is 2000-30001x, light application time is obtain the high startup seedling of 1-3cm after 16h/d, 30d;
(3) Multiplying culture with take root: startup seedling is cut into single stem section, be seeded on medium MS+ sucrose 30g/L+ agar 5g/L+GA34-5mg/L+0.2mg/L NAA, condition of culture is 25 DEG C ± 1 DEG C, intensity of illumination is 2000-30001x, light application time is obtain the cordate houttuynia seedling of taking root that high 6-8cm is with 5-6 stipes after 16h/d, 30d;
(4) hardening and transplanting: the cordate houttuynia seedling that step (3) obtains is carried out acclimatization and transplants.
Utilize the medium that above-described embodiment and comparative example are made, carry out tissue cultures to cordate houttuynia, embodiment Multiplying culture and the time of taking root, comparative example Multiplying culture and the time of taking root were about 30d at 10-16d.
Claims (2)
1. a cordate houttuynia culture medium for tissue culture, this medium comprises subculture medium and root media, it is characterized in that:
The component of often liter of (L) subculture medium and the content of each component as follows:
(1) macroelement: potassium nitrate 1800mg/L, ammonium nitrate 1350mg/L, calcium chloride dihydrate 350mg/L, epsom salt 320mg/L, potassium dihydrogen phosphate 150mg/L, ferrous sulfate heptahydrate 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassium iodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, vitamin b3 (VB
2) 6.0mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, thiamine hydrochloride (VB
1) 0.1mg/L;
(4) plant growth regulator: 6-benzyladenine 0.5-2.0mg/L, methyl α-naphthyl acetate 0.2-0.8mg/L, longan juice 0.3-0.5mg/L;
Surplus is distilled water.
2. cordate houttuynia culture medium for tissue culture according to claim 1, is characterized in that:
The component of often liter of (L) root media and the content of each component as follows:
(1) macroelement: potassium nitrate 900mg/L, ammonium nitrate 675mg/L, calcium chloride dihydrate 175mg/L, epsom salt 160mg/L, potassium dihydrogen phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 21.5mg/L, white vitriol 8.3mg/L, boric acid 6mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 6H2O 0.02mg/L;
(3) organic matter: inositol 80.0mg/L, glycine 2.5mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.3-0.6mg/L, longan juice 0.3-0.5mg/L;
Surplus is distilled water;
Described longan juice is that fresh longan removes branches and leaves, sick wormed fruit, decayed fruit, rotten fruit, crude fruit, and cleaning, removal pericarp fruit stone, pulp squeezes, and the fruit juice obtained, sterilizing obtains.
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