CN101642053A - Cold-resistant anthurium induction culturing method - Google Patents
Cold-resistant anthurium induction culturing method Download PDFInfo
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- CN101642053A CN101642053A CN200910102073A CN200910102073A CN101642053A CN 101642053 A CN101642053 A CN 101642053A CN 200910102073 A CN200910102073 A CN 200910102073A CN 200910102073 A CN200910102073 A CN 200910102073A CN 101642053 A CN101642053 A CN 101642053A
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- anthurium
- cold
- resistant
- calli
- medium
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- 241001312221 Anthurium Species 0.000 title claims abstract description 24
- 238000012258 culturing Methods 0.000 title claims abstract description 17
- 230000006698 induction Effects 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 14
- 239000012879 subculture medium Substances 0.000 claims abstract description 10
- 238000005286 illumination Methods 0.000 claims abstract description 8
- 230000004069 differentiation Effects 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims abstract description 4
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 3
- 241001677259 Acanthophoenix rubra Species 0.000 claims description 26
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 21
- 239000002609 medium Substances 0.000 claims description 17
- 229920001817 Agar Polymers 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 239000008272 agar Substances 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 7
- 230000001488 breeding effect Effects 0.000 claims description 7
- 230000001939 inductive effect Effects 0.000 claims description 7
- 230000004083 survival effect Effects 0.000 claims description 6
- 238000012546 transfer Methods 0.000 claims description 6
- 239000006160 differential media Substances 0.000 claims description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 4
- 238000011156 evaluation Methods 0.000 claims description 3
- 230000000763 evoking effect Effects 0.000 claims description 3
- 239000004471 Glycine Substances 0.000 claims description 2
- 229930003451 Vitamin B1 Natural products 0.000 claims description 2
- 206010020718 hyperplasia Diseases 0.000 claims description 2
- 229940064880 inositol 100 mg Drugs 0.000 claims description 2
- 229960003512 nicotinic acid Drugs 0.000 claims description 2
- 235000001968 nicotinic acid Nutrition 0.000 claims description 2
- 239000011664 nicotinic acid Substances 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 229960003495 thiamine Drugs 0.000 claims description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 2
- 235000010374 vitamin B1 Nutrition 0.000 claims description 2
- 239000011691 vitamin B1 Substances 0.000 claims description 2
- 229940033203 vitamin b6 0.5 mg Drugs 0.000 claims description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract 2
- 230000035755 proliferation Effects 0.000 abstract 2
- 239000012881 co-culture medium Substances 0.000 abstract 1
- 230000002595 cold damage Effects 0.000 abstract 1
- 239000012883 rooting culture medium Substances 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 3
- 238000012827 research and development Methods 0.000 description 2
- 244000127818 Phalaenopsis amabilis Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a cold-resistant anthurium induction culturing method which comprises the following steps of: selecting tender shoots of a main-bred anthurium variety as explants; inoculatingthe tender shoots into an induction culture medium to form calli; selecting the calli which are formed for 2 weeks after the induction; transferring the calli into a co-culture medium for 4-week culturing; selecting newly-generated calli; transferring the newly-generated calli into a subculture medium for culturing till a fast proliferation period; carrying out 2-week selection by exposing the calli to such conditions as a temperature of 5 DEG C and 2000Lux illumination; selecting and transferring the survived calli into the subculture medium for 4-week culturing; carrying out 2-week selection by exposing the calli to such conditions as a temperature of 0 DEG C and 2000Lux illumination; selecting and transferring the survived calli into a differentiation culture medium for differentiationand seedling formation; selecting and transferring the differentiated seedlings into a rooting culture medium so that the differentiated seedlings can grow into normal seedlings; continuing to grow the selected anthurium plants; continuing the growth and assessing and verifying the expression stability of the cold resistance; carrying out proliferation on the stable and fine plants; and culturingthe transgenic cold-resistant anthurium which resists the cold damage at low temperatures.
Description
Technical field
The present invention relates to a kind of cold-resistant anthurium induction culturing method.
Background technology
The red palm (Anthurium andraenum) claim fancy candles lit in the bridal chamber at wedding, An Zuhua again, is Araeceae Anthurium herbaceos perennial, is listed in main cutting flower variety in many tropic countries and area, is to be only second to second blue large tropical flowers commodity of the torrid zone.On Dutch flowers auction marketplace in 2002, the red palm is one of 10 kinds of most popular cut-flowers, is again one of 10 kinds of most popular pot plants, and the sales volume of the red palm of cut-flower reaches 4,160 ten thousand Euros, is fastest-rising in all cutting flower varieties; The sales volume of the red palm of potted flower is only second to Moth orchid, reaches 3,410 ten thousand Euros.
Holland, Israel, Hawaii, America are the main cultivation centers of the present red palm, and especially Holland occupies first place at aspects such as red palm breeding and standardized plantings.The red palm industry of China experiences 20 years of development, obtained than much progress at aspects such as production scale and research and development strengths, but overall research and development and sale and world level is far apart.Although all there is production in a lot of places, still there are not red palm new varieties with independent intellectual property right, its seedling is mainly from abroad.
The cultivation of red palm new varieties has important practical significance to competitiveness and the economic benefit of improving the red palm industrial chain of China, raising industry.Meanwhile, owing to causing the red palm, the reason of China's winter low temperature is difficult to the natural environment plantation at the red palm of China, manually add the temperature control plantation under the greenhouse experiment, not only increase substantially production cost, and low temperature freezing-disaster has also significantly reduced viewing and admiring and commercial quality of the red palm.
Under this background, the present invention proposes a kind of cold-resistant anthurium induction culturing method just, is intended to cultivate the cold-resistant anthurium new varieties, significantly reduces the production cost of the red palm, and raising is viewed and admired and commercial quality, thereby promotes the industrialized development of the red palm.
Summary of the invention
The object of the present invention is to provide a kind of cold-resistant anthurium induction culturing method, to obtain to be more suitable for the red palm new varieties of winter low temperature plantation.
Cold-resistant anthurium induction culturing method is characterized in that may further comprise the steps:
1) getting main tender shoots of planting red palm kind is explant, is seeded to inducing culture, in 25 ℃ of following evoked callus;
2) choose the callus of inducing 2 weeks of formation, be transferred to the common medium that contains ABA, cultivated for 4 weeks altogether;
3) callus of the new hyperplasia of picking is transferred to subculture medium, and successive transfer culture to callus enters the fast breeding phase;
4) choose the callus that is in the fast breeding phase, place 5 ℃ of temperature and illumination 2000Lux to screen for 2 weeks down;
5) choose the still callus of survival, be transferred to subculture medium, successive transfer culture moved to 0 ℃ of temperature and illumination 2000Lux and screened for 2 weeks down after 4 weeks;
6) choose the still callus of survival, be transferred to differential medium, induce seedling differentiation;
7) choose the seedling of differentiation, be transferred to root media and grow up to normal seedling;
8) the red palm strain of continuation plantation step 7) screening is that the expression stability that indoor and outdoor evaluation checking is cold-resistant is bred stable elite plant strain, cultivates cold-resistant anthurium.
Above-mentioned stability is meant that the cold resistance of the selected red palm can express consistently (2 seasons and more than) between different year between (2 years and more than), season and intersite (2 places and more than), and characteristic is not subjected to the appreciable impact in time, season and place.
Among the present invention, said inducing culture adds 2 for the 1/2MS minimal medium, 4-D 0.6mg, 6-BA0.5mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
Among the present invention, said medium altogether adds ABA 5mg/L, 2 for the 1/2MS minimal medium, 4-D0.6mg, 6-BA 2mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
Among the present invention, said subculture medium adds 2 for the MS minimal medium, 4-D 0.6mg, 6-BA2mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
Among the present invention, said differential medium adds KT 1mg, NAA 0.2mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
Among the present invention, said root media adds NAA 0.5mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
Above-mentioned MS minimal medium is macroelement (NH
4NO
3650mg, KNO
3900mg, CaCl
22H
2O 440mg, MgSO
47H
2O 370mg, KH
2PO
4700mg), trace element (KI 0.83mg, H
3BO
36.2mg, MnSO
44H
2O 22.3mg, ZnSO
47H
2O 8.6mg, Na
2MnO
42H2O0.25mg, CuSO
45H
2O 0.025mg, CoCl
26H
2O 0.025mg, FeSO
47H
2O 27.8mgNa
2-EDTA2H
2O 37.3mg) and organic principle (inositol 100mg, nicotinic acid 0.5mg, vitamin B6 0.5mg, vitamin B1 0.5mg, glycine 2mg).
The present invention has the beneficial effect of following several respects:
The cold-resistant anthurium new varieties of cultivating have excellent anti-low temperature freezing-disaster ability than common red palm kind, significantly reduce the production cost of the red palm, improve and view and admire and commercial quality, help promoting quality, save the industrialized development that cost promotes the red palm.
Embodiment
Below further specify the present invention by instantiation.
Embodiment 1:
Tender shoots with red palm kind " doll (Vitara) " is an explant, is seeded to inducing culture, in 25 ℃ of following evoked callus; Choose and induce the callus that formed for 2 weeks, be transferred to the common medium that contains ABA 5mg/L, cultivated for 4 weeks altogether; Picking new life's callus is transferred to and contains subculture medium, and successive transfer culture to callus enters the fast breeding phase; Choose the callus that is in the fast breeding phase, place 5 ℃ of temperature and illumination 2000Lux to screen for 2 weeks down; Choose the still callus of survival, move to subculture medium, successive transfer culture moved to 0 ℃ of temperature and illumination 2000Lux and screened for 2 weeks down after 4 weeks; Choose the still callus of survival, be transferred to the differential medium seedling differentiation; Choose seedling 21 strains of differentiation, be transferred to root media and grow up to normal seedling 8 strains; Continuing the selected red palm strain of plantation is, estimates the cold-resistant expression stability of Simulation evaluation in the 2007-2008 indoor and outdoor, and the cold-resistant anthurium strain that obtains anti-low temperature freezing-disaster is 1, and promptly ICT-Aa-1 can stand 0 ℃ of chilling injury.
Claims (7)
1. cold-resistant anthurium induction culturing method is characterized in that may further comprise the steps:
1) getting main tender shoots of planting red palm kind is explant, is seeded to inducing culture, in 25 ℃ of following evoked callus;
2) choose the callus of inducing 2 weeks of formation, be transferred to the common medium that contains ABA, cultivated for 4 weeks altogether;
3) callus of the new hyperplasia of picking is transferred to subculture medium, and successive transfer culture to callus enters the fast breeding phase;
4) choose the callus that is in the fast breeding phase, place 5 ℃ of temperature and illumination 2000Lux to screen for 2 weeks down;
5) choose the still callus of survival, be transferred to subculture medium, successive transfer culture moved to 0 ℃ of temperature and illumination 2000Lux and screened for 2 weeks down after 4 weeks;
6) choose the still callus of survival, be transferred to differential medium, induce seedling differentiation;
7) choose the seedling of differentiation, be transferred to root media and grow up to normal seedling;
8) the red palm strain of continuation plantation step 7) screening is that the expression stability that indoor and outdoor evaluation checking is cold-resistant is bred stable elite plant strain, cultivates cold-resistant anthurium.
2. cold-resistant anthurium induction culturing method according to claim 1 is characterized in that said inducing culture adds 2 for the 1/2MS minimal medium, 4-D 0.6mg, 6-BA 0.5mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
3. cold-resistant anthurium induction culturing method according to claim 1 is characterized in that said medium altogether adds ABA 5mg/L, 2 for the 1/2MS minimal medium, 4-D 0.6mg, 6-BA 2mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
4. cold-resistant anthurium induction culturing method according to claim 1 is characterized in that said subculture medium adds 2 for the MS minimal medium, 4-D 0.6mg, 6-BA 2mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
5. cold-resistant anthurium induction culturing method according to claim 1 is characterized in that said differential medium adds KT 1mg, NAA 0.2mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
6. cold-resistant anthurium induction culturing method according to claim 1 is characterized in that said root media adds NAA 0.5mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
7. according to arbitrary described cold-resistant anthurium induction culturing method among the claim 2-6, it is characterized in that said MS minimal medium is NH
4NO
3650mg, KNO
3900mg, CaCl
22H
2O 440mg, MgSO
47H
2O 370mg, KH
2PO
4700mg, KI 0.83mg, H
3BO
36.2mg, MnSO
44H
2O22.3mg, ZnSO
47H
2O 8.6mg, Na
2MnO
42H2O 0.25mg, CuSO
45H
2O 0.025mg, CoCl
26H
2O 0.025mg, FeSO
47H
2O 27.8mg Na
2-EDTA2H
2O 37.3mg, inositol 100mg, nicotinic acid 0.5mg, vitamin B6 0.5mg, vitamin B1 0.5mg and glycine 2mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910102073A CN101642053A (en) | 2009-08-25 | 2009-08-25 | Cold-resistant anthurium induction culturing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910102073A CN101642053A (en) | 2009-08-25 | 2009-08-25 | Cold-resistant anthurium induction culturing method |
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| Publication Number | Publication Date |
|---|---|
| CN101642053A true CN101642053A (en) | 2010-02-10 |
Family
ID=41654236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910102073A Pending CN101642053A (en) | 2009-08-25 | 2009-08-25 | Cold-resistant anthurium induction culturing method |
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| Country | Link |
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| CN (1) | CN101642053A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823503A (en) * | 2012-09-26 | 2012-12-19 | 钦州市林业科学研究所 | Tissue culture medium for propagating anthurium buds by using buds |
| CN103053417A (en) * | 2012-12-26 | 2013-04-24 | 中国长江三峡集团公司 | Rapid propagation method for clustered shoot through induction of aerial root of Anthurium andraeanum Lind |
| CN109118488A (en) * | 2018-09-06 | 2019-01-01 | 上海市农业科学院 | A kind of identification method of red palm new germ plasm cold resistance |
-
2009
- 2009-08-25 CN CN200910102073A patent/CN101642053A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823503A (en) * | 2012-09-26 | 2012-12-19 | 钦州市林业科学研究所 | Tissue culture medium for propagating anthurium buds by using buds |
| CN103053417A (en) * | 2012-12-26 | 2013-04-24 | 中国长江三峡集团公司 | Rapid propagation method for clustered shoot through induction of aerial root of Anthurium andraeanum Lind |
| CN109118488A (en) * | 2018-09-06 | 2019-01-01 | 上海市农业科学院 | A kind of identification method of red palm new germ plasm cold resistance |
| CN109118488B (en) * | 2018-09-06 | 2021-07-06 | 上海市农业科学院 | Method for identifying cold resistance of new anthurium andraeanum germplasm |
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Open date: 20100210 |