CN101637129A - Culture method of cold-resistant anthurium andraenum - Google Patents
Culture method of cold-resistant anthurium andraenum Download PDFInfo
- Publication number
- CN101637129A CN101637129A CN200910102070A CN200910102070A CN101637129A CN 101637129 A CN101637129 A CN 101637129A CN 200910102070 A CN200910102070 A CN 200910102070A CN 200910102070 A CN200910102070 A CN 200910102070A CN 101637129 A CN101637129 A CN 101637129A
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- anthurium
- cold
- resistant
- calluses
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- 241001312221 Anthurium Species 0.000 title claims abstract description 24
- 238000012136 culture method Methods 0.000 title abstract 2
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 25
- 230000001939 inductive effect Effects 0.000 claims abstract description 11
- 238000005286 illumination Methods 0.000 claims abstract description 8
- 238000012546 transfer Methods 0.000 claims abstract description 5
- 241000196324 Embryophyta Species 0.000 claims abstract description 4
- 241001677259 Acanthophoenix rubra Species 0.000 claims description 26
- 238000009395 breeding Methods 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 230000004069 differentiation Effects 0.000 claims description 6
- 239000006160 differential media Substances 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- 239000012879 subculture medium Substances 0.000 claims description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 4
- 230000001488 breeding effect Effects 0.000 claims description 4
- GUTLYIVDDKVIGB-OUBTZVSYSA-N Cobalt-60 Chemical compound [60Co] GUTLYIVDDKVIGB-OUBTZVSYSA-N 0.000 claims description 3
- 238000011156 evaluation Methods 0.000 claims description 3
- 230000000763 evoking effect Effects 0.000 claims description 3
- 206010020718 hyperplasia Diseases 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 claims description 3
- 239000004471 Glycine Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 229940064880 inositol 100 mg Drugs 0.000 claims description 2
- 229960003512 nicotinic acid Drugs 0.000 claims description 2
- 235000001968 nicotinic acid Nutrition 0.000 claims description 2
- 239000011664 nicotinic acid Substances 0.000 claims description 2
- 229940033203 vitamin b6 0.5 mg Drugs 0.000 claims description 2
- 229930003451 Vitamin B1 Natural products 0.000 claims 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims 1
- 229960003495 thiamine Drugs 0.000 claims 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims 1
- 235000010374 vitamin B1 Nutrition 0.000 claims 1
- 239000011691 vitamin B1 Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 208000027418 Wounds and injury Diseases 0.000 abstract description 3
- 230000006378 damage Effects 0.000 abstract description 3
- 208000014674 injury Diseases 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 2
- 230000006698 induction Effects 0.000 abstract 1
- 230000001678 irradiating effect Effects 0.000 abstract 1
- 230000000644 propagated effect Effects 0.000 abstract 1
- 230000001902 propagating effect Effects 0.000 abstract 1
- 238000011161 development Methods 0.000 description 3
- 238000012827 research and development Methods 0.000 description 2
- 244000127818 Phalaenopsis amabilis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a culture method of cold-resistant anthurium andraenum, which comprises the following steps: taking the apical bud of the anthurium andraenum of the main variety as explant, and inducing the explant to form calluses; selecting the calluses which are formed for two weeks by induction, irradiating the calluses by gamma rays, and transferring and culturing the calluses to thepropagation period; picking the newly propagated calluses, and putting the calluses at the temperature of 5 DEG C and the illumination of 2000Lux for selecting for two weeks; transferring the calluses to a successive transfer culture medium for successive transfer culture for two weeks, and putting the calluses at the temperature of 0 DEG C and the illumination of 2000Lux for selecting for two weeks; selecting the calluses which are still alive, transferring the calluses to a differential culture medium for differential culture to form sprouts, and transferring the sprouts to a root culture medium to form normal seedlings; and continuously planting the normal seedlings, evaluating and verifying the expression stability of the cold resistance, propagating the stable excellent plants, and then, culturing the cold-resistant anthurium andraenum capable of resisting low temperature and frozen injury. The properties of resisting low temperature and frozen injury of the new variety of the cultured cold-resistant anthurium andraenum are stronger than those of the common anthurium andraenum varieties, the production cost is remarkably reduced, and the ornamental value and the commercial value are improved.
Description
Technical field
The present invention relates to a kind of breeding method of cold-resistant anthurium.
Background technology
The red palm (Anthurium andraenum) claim fancy candles lit in the bridal chamber at wedding, An Zuhua again, is Araeceae Anthurium herbaceos perennial, is listed in main cutting flower variety in many tropic countries and area, is to be only second to second blue large tropical flowers commodity of the torrid zone.On Dutch flowers auction marketplace in 2002, the red palm is one of 10 kinds of most popular cut-flowers, is again one of 10 kinds of most popular pot plants, and the sales volume of the red palm of cut-flower reaches 4,160 ten thousand Euros, is fastest-rising in all cutting flower varieties; The sales volume of the red palm of potted flower is only second to Moth orchid, reaches 3,410 ten thousand Euros.
Holland, Israel, Hawaii, America are the main cultivation centers of the present red palm, and especially Holland occupies first place at aspects such as red palm breeding and standardized plantings.The red palm industry of China experiences 20 years of development, obtained than much progress at aspects such as production scale and research and development strengths, but overall research and development and sale and world level is far apart.Although all there is production in a lot of places, still there are not red palm new varieties with independent intellectual property right, its seedling is mainly from abroad.
The cultivation of red palm new varieties has important practical significance to competitiveness and the economic benefit of improving the red palm industrial chain of China, raising industry.Meanwhile, owing to causing the red palm, the reason of China's winter low temperature is difficult to the natural environment plantation at the red palm of China, manually add the temperature control plantation under the greenhouse experiment, not only increase substantially production cost, and low temperature freezing-disaster has also significantly reduced viewing and admiring and commercial quality of the red palm.
Under this background, the present invention proposes a kind of breeding method of cold-resistant anthurium just, is intended to cultivate the cold-resistant anthurium new varieties, significantly reduces the production cost of the red palm, and raising is viewed and admired and commercial quality, thereby promotes the industrialized development of the red palm.
Summary of the invention
The object of the present invention is to provide a kind of breeding method of cold-resistant anthurium, to obtain to be more suitable for the red palm new varieties of winter low temperature plantation.
The breeding method of cold-resistant anthurium may further comprise the steps:
1) getting main terminal bud of planting red palm kind is explant, is seeded to inducing culture, in 25 ℃ of following evoked callus;
2) choose the callus of inducing 2 weeks of formation, the cobalt-60 gamma rays irradiation with 5Gy is transferred to inducing culture thereafter, is cultured to callus and enters the fast breeding phase;
3) callus of the new hyperplasia of picking places 5 ℃ of temperature and illumination 2000Lux to screen for 2 weeks down, moves to subculture medium thereafter, in 2 weeks of successive transfer culture, places 2 weeks of screening under 0 ℃ of temperature and the illumination 2000Lux;
4) choose the still callus of survival, be transferred to the differential medium seedling differentiation;
5) choose the seedling of differentiation, be transferred to root media and grow up to normal seedling;
6) the red palm strain of continuation plantation step 5) screening is, the expression stability of indoor and outdoor evaluation checking cold resistance is bred stable elite plant strain, cultivates the cold-resistant anthurium of anti-low temperature freezing-disaster.
Above-mentioned stability is meant that the cold resistance of the selected red palm can express consistently (2 seasons and more than) between different year between (2 years and more than), season and intersite (2 places and more than), and characteristic is not subjected to the appreciable impact in time, season and place.
Among the present invention, said inducing culture adds 2 for the 1/2MS minimal medium, 4-D 0.6mg, 6-BA0.5mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
Among the present invention, said subculture medium adds 2 for the MS minimal medium, 4-D 0.6mg, 6-BA2mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
Among the present invention, said differential medium adds KT 1mg, NAA 0.2mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
Among the present invention, said root media adds NAA 0.5mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
Above-mentioned MS minimal medium is macroelement (NH
4NO
3650mg, KNO
3900mg, CaCl
22H
2O 440mg, MgSO
47H
2O 370mg, KH
2PO
4700mg), trace element (KI 0.83mg, H
3BO
36.2mg, MnSO
44H
2O 22.3mg, ZnSO
47H
2O 8.6mg, Na
2MnO
42H2O0.25mg, CuSO
45H
2O 0.025mg, CoCl
26H
2O 0.025mg, FeSO
47H
2O 27.8mgNa
2-EDTA2H
2O 37.3mg) and organic principle (inositol 100mg, nicotinic acid 0.5mg, vitamin B6 0.5mg, vitaminB10 .5mg, glycine 2mg).
The present invention has the beneficial effect of following several respects:
The cold-resistant anthurium new varieties of cultivating have excellent anti-low temperature freezing-disaster ability than common red palm kind, significantly reduce the production cost of the red palm, improve and view and admire and commercial quality, help promoting quality, save the industrialized development that cost promotes the red palm.
Embodiment
Below further specify the present invention by instantiation.
Embodiment 1:
Terminal bud with red palm kind " flame " is an explant, is seeded to inducing culture, in 25 ℃ of following evoked callus; Choose and induce the callus that formed for 2 weeks, the cobalt-60 gamma rays irradiation with 5Gy is transferred to inducing culture thereafter, is cultured to callus and enters the fast breeding phase; The callus of the new hyperplasia of picking places 5 ℃ of temperature and illumination 2000Lux to screen for 2 weeks down, moves to subculture medium thereafter, in 2 weeks of successive transfer culture, places 2 weeks of screening under 0 ℃ of temperature and the illumination 2000Lux; Choose the still callus of survival, be transferred to differential medium and induce seedling differentiation; Choose seedling 87 strains of differentiation, be transferred to root media and grow up to normal seedling 54 strains; Continuing the selected red palm strain of plantation is, estimates the cold-resistant expression stability of Simulation evaluation in the 2007-2008 indoor and outdoor, and the cold-resistant anthurium strain that obtains anti-low temperature freezing-disaster is 2, and promptly CT-Aa-1 and CT-Aa-2 can stand 0 ℃ of chilling injury.
Claims (6)
1. the breeding method of a cold-resistant anthurium is characterized in that may further comprise the steps:
1) getting main terminal bud of planting red palm kind is explant, is seeded to inducing culture, in 25 ℃ of following evoked callus;
2) choose the callus of inducing 2 weeks of formation, the cobalt-60 gamma rays irradiation with 5Gy is transferred to inducing culture thereafter, is cultured to callus and enters the fast breeding phase;
3) callus of the new hyperplasia of picking places 5 ℃ of temperature and illumination 2000Lux to screen for 2 weeks down, moves to subculture medium thereafter, in 2 weeks of successive transfer culture, places 2 weeks of screening under 0 ℃ of temperature and the illumination 2000Lux;
4) choose the still callus of survival, be transferred to the differential medium seedling differentiation;
5) choose the seedling of differentiation, be transferred to root media and grow up to normal seedling;
6) the red palm strain of continuation plantation step 5) screening is, the expression stability of indoor and outdoor evaluation checking cold resistance is bred stable elite plant strain, cultivates the cold-resistant anthurium of anti-low temperature freezing-disaster.
2. the breeding method of cold-resistant anthurium according to claim 1 is characterized in that said inducing culture adds 2 for the 1/2MS minimal medium, 4-D 0.6mg, 6-BA 0.5mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
3. the breeding method of cold-resistant anthurium according to claim 1 is characterized in that said subculture medium adds 2 for the MS minimal medium, 4-D 0.6mg, 6-BA 2mg, sucrose 30g, agar powder 6.8g, pH sterilization preceding 5.8.
4. the breeding method of cold-resistant anthurium according to claim 1 is characterized in that said differential medium adds KT 1mg, NAA 0.2mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
5. the breeding method of cold-resistant anthurium according to claim 1 is characterized in that said root media adds NAA 0.5mg, sucrose 30g, agar powder 6.8g for the MS minimal medium, pH sterilization preceding 5.8.
6. according to the breeding method of arbitrary described cold-resistant anthurium among the claim 2-5, it is characterized in that said MS minimal medium is NH
4NO
3650mg, KNO
3900mg, CaCl
22H
2O 440mg, MgSO
47H
2O 370mg, KH
2PO
4700mg, KI 0.83mg, H
3BO
36.2mg, MnSO
44H
2O22.3mg, ZnSO
47H
2O 8.6mg, Na
2MnO
42H2O 0.25mg, CuSO
45H
2O 0.025mg, CoCl
26H
2O 0.025mg, FeSO
47H
2O 27.8mg Na
2-EDTA2H
2O 37.3mg, inositol 100mg, nicotinic acid 0.5mg, vitamin B6 0.5mg, vitamin B1 0.5mg and glycine 2mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910102070A CN101637129A (en) | 2009-08-25 | 2009-08-25 | Culture method of cold-resistant anthurium andraenum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910102070A CN101637129A (en) | 2009-08-25 | 2009-08-25 | Culture method of cold-resistant anthurium andraenum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101637129A true CN101637129A (en) | 2010-02-03 |
Family
ID=41612453
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910102070A Pending CN101637129A (en) | 2009-08-25 | 2009-08-25 | Culture method of cold-resistant anthurium andraenum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101637129A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340043A (en) * | 2013-07-05 | 2013-10-09 | 黑龙江省科学院技术物理研究所 | Method for improving cold resistance of japonica rice at sprout period by performing irradiation treatment on japonica rice seeds with low-dose electron rays |
| CN104782460A (en) * | 2015-04-09 | 2015-07-22 | 广西大学 | Culture medium for anthurium |
| CN106386486A (en) * | 2016-09-06 | 2017-02-15 | 广州市名卉景观科技发展有限公司 | Anthurium tissue culture and fast propagation culture medium and anthurium tissue culture and fast propagation seed production method |
| CN107135758A (en) * | 2017-03-27 | 2017-09-08 | 苏州大学 | A kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance |
| CN111201981A (en) * | 2020-03-10 | 2020-05-29 | 云南省烟草农业科学研究院 | Flue-cured tobacco cultivation management and baking method for relieving cold damage based on monopotassium phosphate and sucrose |
-
2009
- 2009-08-25 CN CN200910102070A patent/CN101637129A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340043A (en) * | 2013-07-05 | 2013-10-09 | 黑龙江省科学院技术物理研究所 | Method for improving cold resistance of japonica rice at sprout period by performing irradiation treatment on japonica rice seeds with low-dose electron rays |
| CN104782460A (en) * | 2015-04-09 | 2015-07-22 | 广西大学 | Culture medium for anthurium |
| CN106386486A (en) * | 2016-09-06 | 2017-02-15 | 广州市名卉景观科技发展有限公司 | Anthurium tissue culture and fast propagation culture medium and anthurium tissue culture and fast propagation seed production method |
| CN107135758A (en) * | 2017-03-27 | 2017-09-08 | 苏州大学 | A kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance |
| CN111201981A (en) * | 2020-03-10 | 2020-05-29 | 云南省烟草农业科学研究院 | Flue-cured tobacco cultivation management and baking method for relieving cold damage based on monopotassium phosphate and sucrose |
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Open date: 20100203 |