CN109349109A - Shaping methods in the bottle of alpine rose young beauty - Google Patents

Shaping methods in the bottle of alpine rose young beauty Download PDF

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Publication number
CN109349109A
CN109349109A CN201811403140.8A CN201811403140A CN109349109A CN 109349109 A CN109349109 A CN 109349109A CN 201811403140 A CN201811403140 A CN 201811403140A CN 109349109 A CN109349109 A CN 109349109A
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CN
China
Prior art keywords
seedling
bottle
culture
alpine rose
lateral bud
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811403140.8A
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Chinese (zh)
Inventor
彭绿春
瞿素萍
解玮佳
李世峰
宋杰
李树发
蔡艳飞
张露
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Flower Research Institute of YAAS
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Flower Research Institute of YAAS
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Priority to CN201811403140.8A priority Critical patent/CN109349109A/en
Publication of CN109349109A publication Critical patent/CN109349109A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

Abstract

Shaping methods in the bottle of alpine rose young beauty, this method is to alpine rose young beauty's tissue culture proliferation seedling using the combination processing of material cutting mode and the induction of lateral bud former base before carrying out culture of rootage, plant shaping is realized in bottle, finished product Plug seedling has 3 side shoots, eliminate the topping shaping step for the first time of potted flower seedling and afforestation seedling, shorten the growth cycle time 46 days, production cost is reduced, the industrialized development of alpine rose young beauty seedling, potted flower and afforestation product is promoted.

Description

Shaping methods in the bottle of alpine rose young beauty
Technical field
The invention belongs to plant tissue culture seeling industry technical fields, and in particular to arrive alpine rose young beauty tissue culture seedling Shaping methods in bottle.
Background technique
Alpine rose is the woody flower of one of big famous flower in China ten and world pop, has become China year in recent years Night is colored and the rising star of Top-grade-landscaping, but because of potted flower seedling and Nursery Stock very long nurturing period, it is caused to be produced into This is high, and production scale is limited, becomes one of the factor for restricting its industrialization production.Alpine rose seedling mainly passes through group The method production for knitting culture, generally comprises explant induction, proliferation, three steps of taking root, wherein the way taken root is that will be proliferated Seedling is cut into single plant and is inoculated into root media.The bottle seedling of taking root of tissue culture is transplanted to hole tray, and finished product Plug seedling, which is transplanted to cultivation, to be held After device (flowerpot), 3 side shoots can be formed by pinching for the first time.Since single plant is seeded in root media, until shaping becomes tool 3 Production cycle needed for the basin seedling of a hard side shoot is 280 days.
Young beauty's wide adaptability, resistance, pattern are bright-coloured, flower is in great numbers, leaf color is dark green, floral leaf is enjoyed together, and have higher Ornamental value, be one of nearly higher alpine rose kind of 2 years tractions, be widely used in private savings garden, city flower Garden and pot flowers, are well received by consumers.
Summary of the invention
To shorten alpine rose young beauty potted flower nursery stock and Nursery Stock production cycle, production cost is reduced, originally Invention provides shaping methods in the bottle of alpine rose young beauty a kind of, and this method mainly includes the cutting of proliferation seedling and lateral bud former base Induction, concrete operations are:
(1) proliferation seedling cutting
The long alpine rose young beauty's tissue culture to 4cm or more of plant height is proliferated seedling, single plant is cut into, cuts off single plant stem Point, then single plant is cut into stem section, and 3 functional leafs of every stem section band;
(2) lateral bud former base induces
The stem section being cut into, which is forwarded in lateral bud former base induced medium at culture to the visible axil of naked eyes, lateral bud eruption, Condition of culture are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, the lateral bud former base induction training Support base are as follows: WPM+ZT 1mg/L+NAA0.05mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4;
Above-mentioned be forwarded in root media through lateral bud former base induction stem section of eruption lateral bud at axil is taken root Culture carries out practicing seedling after culture of rootage, cultivates Plug seedling, basin on Plug seedling.
Further, the culture of rootage is culture 20 days, culture of rootage condition of culture are as follows: 23 DEG C of temperature, 2 DEG C of scholar, illumination is strong Spend 2000-2500lx, light application time 12h/d, root media are as follows: WPM+NAA1mg/L+IBA0.75mg/L+ sucrose 30g/L+ Agar 7g/L, pH value 5.4.
Further, the experienced seedling is to move to the bottle seedling band bottle through culture of rootage in greenhouse, intensity of illumination 5000~ Under the conditions of 8000lx, practice seedling 7 days.
Further, the cultivation Plug seedling is taken out bottle seedling out of bottle after experienced seedling, and the IBA of the quickly dipped 8g/L in bottle seedling root is molten After liquid, implantation fills the Plug seedling for routinely managing and being cultivated to 3 side shoots of band in the hole tray of cultivation matrix.
Further, the cultivation matrix in hole tray is turf and perlite in turf: the volume ratio of perlite is that 6:1 ratio is mixed It closes.
Further, basin is that growth forms 3, band in the flowerpot that bore is 11cm by hole tray transplantation of seedlings on the Plug seedling The finished product basin seedling of hard side shoot.
Compared with prior art, the beneficial effects of the present invention are:
The prior art is usually in alpine rose tissue-cultured seedling by practicing seedling, cultivating Plug seedling, right for the first time on Plug seedling after basin Its shaping of pinching, this, which is operated so that alpine rose is formed, has the time of side shoot plant type longer, it usually needs and 120 days or more, and The present invention carries out material to the proliferation seedling of tissue culture that is, before tissue culture culture of rootage using shaping is carried out in bottle before tissue culture culture of rootage Material cutting mode (cutting mode that proliferation seedling is cut into single plant, cuts stem apex, is cut into three functional leaf stem sections of band) and " lateral bud original The coordinated of two steps of base induction ", realizes plant shaping in tissue culture bottle, so that the Plug seedling after transplanting has 3 A side shoot eliminates the conventional topping shaping step for the first time to potted flower seedling and afforestation seedling, shortens the production cycle 46 days, Production cost is reduced, unexpected technical effect is produced, promotes alpine rose young beauty seedling, potted flower and gardens Afforest the industrialized development of product.
Specific embodiment:
Material used in following embodiment and reagent be it is commercially available, each embodiment without specified otherwise be conventional method.
Shaping methods in the bottle of alpine rose young beauty provided by the invention, the specific steps are as follows:
(1) proliferation seedling cutting
It is routinely after alpine rose method for tissue culture culture alpine rose young beauty, plant height is long to 4cm's or more Young beauty's tissue culture is proliferated seedling, is cut into single plant, cuts off single plant stem apex, single plant is cut into stem section, and 3 function of every stem section band later It can leaf.
(2) lateral bud former base induces
The stem section being cut into, which is forwarded in lateral bud former base induced medium at culture to the visible axil of naked eyes, lateral bud eruption, Condition of culture are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, the lateral bud former base induction training Support base are as follows: WPM+ZT 1mg/L+NAA0.05mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4;
(3) culture of rootage
By the stem section of eruption lateral bud is forwarded in root media and cultivates 20 days at axil in step (2), culture of rootage item Part are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, root media are as follows: WPM+NAA1mg/ L+IBA0.75mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4.
(4) it takes root transplantation of seedlings
Culture of rootage 20 days in step (3) bottle seedling band bottles (not opening bottle cap) are moved to and are practiced in outdoor greenhouse seedling 7 days, light According to intensity about 5000-8000lx, bottle seedling is taken out out of bottle later, after the IBA solution of the quickly dipped 8g/L in bottle seedling root, implantation cultivation In matrix (cultivation matrix is turf and perlite by turf: the volume ratio of perlite is that 6:1 is mixed), conventional daily management, 160 The finished product Plug seedling for 3 side shoots of band is cultivated after it.Hole tray transplantation of seedlings was in the flowerpot of bore 11cm, through further growth in 40 days Form the finished product basin seedling of 3, band hard side shoots.
Shaping seeling industry effect in 1 alpine rose young beauty's bottle of table
Note:
Bottle seedling of the taking root production cycle refers to that plant is seeded in root media and starts, until rooted seedling can transplant the required time.
The Plug seedling production cycle refers to since being transplanted in hole tray bottle seedling of taking root, until it is lifted up plant, cultivation matrix energy Completely from the time needed for cavities abjection.
The time that 3 hard side shoots are formed in flowerpot refers to that finished product Plug seedling is transplanted to flowerpot and starts, until forming 3 hard side shoot institutes The time needed.
Total production cycle refers to the sum of every cultivation production time in table 1.

Claims (6)

1. shaping methods in the bottle of alpine rose young beauty, which is characterized in that it is induced including the cutting of proliferation seedling and lateral bud former base, Its concrete operations is:
(1) proliferation seedling cutting
The long alpine rose young beauty's tissue culture to 4cm or more of plant height is proliferated seedling, single plant is cut into, cuts off single plant stem apex, then Single plant is cut into stem section, and 3 functional leafs of every stem section band;
(2) lateral bud former base induces
The stem section being cut into, which is forwarded in lateral bud former base induced medium at culture to the visible axil of naked eyes, lateral bud eruption, cultivates Condition are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, the lateral bud former base induced medium Are as follows: WPM+ZT 1mg/L+NAA 0.05mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4;
Culture of rootage is carried out by being forwarded in root media through lateral bud former base induction stem section of eruption lateral bud at axil, is taken root It carries out practicing seedling after culture, cultivate Plug seedling, basin on Plug seedling.
2. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the training of taking root Support culture 20 days, culture of rootage condition are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d are raw Root culture medium are as follows: WPM+NAA 1mg/L+IBA 0.75mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4.
3. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the experienced seedling is Bottle seedling band bottle through culture of rootage is moved in greenhouse, under the conditions of intensity of illumination 5000-8000lx, is practiced seedling 7 days.
4. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the cultivation cave Disk seedling is taken out bottle seedling out of bottle after experienced seedling, and after the IBA solution of the quickly dipped 8g/L in bottle seedling root, implantation fills the cave of cultivation matrix Routinely management is cultivated to the Plug seedling of 3 side shoots of band in disk.
5. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the Plug seedling Upper basin is that growth forms the finished product basin seedling of 3, band hard side shoots in the flowerpot that bore is 11cm by hole tray transplantation of seedlings.
6. shaping methods in the bottle of alpine rose young beauty according to claim 4, which is characterized in that cultivated in hole tray Matrix is turf and perlite in turf: the volume ratio of perlite is that 6:1 ratio mixes.
CN201811403140.8A 2018-11-23 2018-11-23 Shaping methods in the bottle of alpine rose young beauty Pending CN109349109A (en)

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Publication number Priority date Publication date Assignee Title
CN113767848A (en) * 2021-08-25 2021-12-10 山东省农业科学院 Efficient rooting method for rhododendron lapponicum tissue culture seedlings

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