CN109349109A - Shaping methods in the bottle of alpine rose young beauty - Google Patents
Shaping methods in the bottle of alpine rose young beauty Download PDFInfo
- Publication number
- CN109349109A CN109349109A CN201811403140.8A CN201811403140A CN109349109A CN 109349109 A CN109349109 A CN 109349109A CN 201811403140 A CN201811403140 A CN 201811403140A CN 109349109 A CN109349109 A CN 109349109A
- Authority
- CN
- China
- Prior art keywords
- seedling
- bottle
- culture
- alpine rose
- lateral bud
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Shaping methods in the bottle of alpine rose young beauty, this method is to alpine rose young beauty's tissue culture proliferation seedling using the combination processing of material cutting mode and the induction of lateral bud former base before carrying out culture of rootage, plant shaping is realized in bottle, finished product Plug seedling has 3 side shoots, eliminate the topping shaping step for the first time of potted flower seedling and afforestation seedling, shorten the growth cycle time 46 days, production cost is reduced, the industrialized development of alpine rose young beauty seedling, potted flower and afforestation product is promoted.
Description
Technical field
The invention belongs to plant tissue culture seeling industry technical fields, and in particular to arrive alpine rose young beauty tissue culture seedling
Shaping methods in bottle.
Background technique
Alpine rose is the woody flower of one of big famous flower in China ten and world pop, has become China year in recent years
Night is colored and the rising star of Top-grade-landscaping, but because of potted flower seedling and Nursery Stock very long nurturing period, it is caused to be produced into
This is high, and production scale is limited, becomes one of the factor for restricting its industrialization production.Alpine rose seedling mainly passes through group
The method production for knitting culture, generally comprises explant induction, proliferation, three steps of taking root, wherein the way taken root is that will be proliferated
Seedling is cut into single plant and is inoculated into root media.The bottle seedling of taking root of tissue culture is transplanted to hole tray, and finished product Plug seedling, which is transplanted to cultivation, to be held
After device (flowerpot), 3 side shoots can be formed by pinching for the first time.Since single plant is seeded in root media, until shaping becomes tool 3
Production cycle needed for the basin seedling of a hard side shoot is 280 days.
Young beauty's wide adaptability, resistance, pattern are bright-coloured, flower is in great numbers, leaf color is dark green, floral leaf is enjoyed together, and have higher
Ornamental value, be one of nearly higher alpine rose kind of 2 years tractions, be widely used in private savings garden, city flower
Garden and pot flowers, are well received by consumers.
Summary of the invention
To shorten alpine rose young beauty potted flower nursery stock and Nursery Stock production cycle, production cost is reduced, originally
Invention provides shaping methods in the bottle of alpine rose young beauty a kind of, and this method mainly includes the cutting of proliferation seedling and lateral bud former base
Induction, concrete operations are:
(1) proliferation seedling cutting
The long alpine rose young beauty's tissue culture to 4cm or more of plant height is proliferated seedling, single plant is cut into, cuts off single plant stem
Point, then single plant is cut into stem section, and 3 functional leafs of every stem section band；
(2) lateral bud former base induces
The stem section being cut into, which is forwarded in lateral bud former base induced medium at culture to the visible axil of naked eyes, lateral bud eruption,
Condition of culture are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, the lateral bud former base induction training
Support base are as follows: WPM+ZT 1mg/L+NAA0.05mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4；
Above-mentioned be forwarded in root media through lateral bud former base induction stem section of eruption lateral bud at axil is taken root
Culture carries out practicing seedling after culture of rootage, cultivates Plug seedling, basin on Plug seedling.
Further, the culture of rootage is culture 20 days, culture of rootage condition of culture are as follows: 23 DEG C of temperature, 2 DEG C of scholar, illumination is strong
Spend 2000-2500lx, light application time 12h/d, root media are as follows: WPM+NAA1mg/L+IBA0.75mg/L+ sucrose 30g/L+
Agar 7g/L, pH value 5.4.
Further, the experienced seedling is to move to the bottle seedling band bottle through culture of rootage in greenhouse, intensity of illumination 5000~
Under the conditions of 8000lx, practice seedling 7 days.
Further, the cultivation Plug seedling is taken out bottle seedling out of bottle after experienced seedling, and the IBA of the quickly dipped 8g/L in bottle seedling root is molten
After liquid, implantation fills the Plug seedling for routinely managing and being cultivated to 3 side shoots of band in the hole tray of cultivation matrix.
Further, the cultivation matrix in hole tray is turf and perlite in turf: the volume ratio of perlite is that 6:1 ratio is mixed
It closes.
Further, basin is that growth forms 3, band in the flowerpot that bore is 11cm by hole tray transplantation of seedlings on the Plug seedling
The finished product basin seedling of hard side shoot.
Compared with prior art, the beneficial effects of the present invention are:
The prior art is usually in alpine rose tissue-cultured seedling by practicing seedling, cultivating Plug seedling, right for the first time on Plug seedling after basin
Its shaping of pinching, this, which is operated so that alpine rose is formed, has the time of side shoot plant type longer, it usually needs and 120 days or more, and
The present invention carries out material to the proliferation seedling of tissue culture that is, before tissue culture culture of rootage using shaping is carried out in bottle before tissue culture culture of rootage
Material cutting mode (cutting mode that proliferation seedling is cut into single plant, cuts stem apex, is cut into three functional leaf stem sections of band) and " lateral bud original
The coordinated of two steps of base induction ", realizes plant shaping in tissue culture bottle, so that the Plug seedling after transplanting has 3
A side shoot eliminates the conventional topping shaping step for the first time to potted flower seedling and afforestation seedling, shortens the production cycle 46 days,
Production cost is reduced, unexpected technical effect is produced, promotes alpine rose young beauty seedling, potted flower and gardens
Afforest the industrialized development of product.
Specific embodiment:
Material used in following embodiment and reagent be it is commercially available, each embodiment without specified otherwise be conventional method.
Shaping methods in the bottle of alpine rose young beauty provided by the invention, the specific steps are as follows:
(1) proliferation seedling cutting
It is routinely after alpine rose method for tissue culture culture alpine rose young beauty, plant height is long to 4cm's or more
Young beauty's tissue culture is proliferated seedling, is cut into single plant, cuts off single plant stem apex, single plant is cut into stem section, and 3 function of every stem section band later
It can leaf.
(2) lateral bud former base induces
The stem section being cut into, which is forwarded in lateral bud former base induced medium at culture to the visible axil of naked eyes, lateral bud eruption,
Condition of culture are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, the lateral bud former base induction training
Support base are as follows: WPM+ZT 1mg/L+NAA0.05mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4；
(3) culture of rootage
By the stem section of eruption lateral bud is forwarded in root media and cultivates 20 days at axil in step (2), culture of rootage item
Part are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, root media are as follows: WPM+NAA1mg/
L+IBA0.75mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4.
(4) it takes root transplantation of seedlings
Culture of rootage 20 days in step (3) bottle seedling band bottles (not opening bottle cap) are moved to and are practiced in outdoor greenhouse seedling 7 days, light
According to intensity about 5000-8000lx, bottle seedling is taken out out of bottle later, after the IBA solution of the quickly dipped 8g/L in bottle seedling root, implantation cultivation
In matrix (cultivation matrix is turf and perlite by turf: the volume ratio of perlite is that 6:1 is mixed), conventional daily management, 160
The finished product Plug seedling for 3 side shoots of band is cultivated after it.Hole tray transplantation of seedlings was in the flowerpot of bore 11cm, through further growth in 40 days
Form the finished product basin seedling of 3, band hard side shoots.
Shaping seeling industry effect in 1 alpine rose young beauty's bottle of table
Note:
Bottle seedling of the taking root production cycle refers to that plant is seeded in root media and starts, until rooted seedling can transplant the required time.
The Plug seedling production cycle refers to since being transplanted in hole tray bottle seedling of taking root, until it is lifted up plant, cultivation matrix energy
Completely from the time needed for cavities abjection.
The time that 3 hard side shoots are formed in flowerpot refers to that finished product Plug seedling is transplanted to flowerpot and starts, until forming 3 hard side shoot institutes
The time needed.
Total production cycle refers to the sum of every cultivation production time in table 1.
Claims (6)
1. shaping methods in the bottle of alpine rose young beauty, which is characterized in that it is induced including the cutting of proliferation seedling and lateral bud former base,
Its concrete operations is:
(1) proliferation seedling cutting
The long alpine rose young beauty's tissue culture to 4cm or more of plant height is proliferated seedling, single plant is cut into, cuts off single plant stem apex, then
Single plant is cut into stem section, and 3 functional leafs of every stem section band；
(2) lateral bud former base induces
The stem section being cut into, which is forwarded in lateral bud former base induced medium at culture to the visible axil of naked eyes, lateral bud eruption, cultivates
Condition are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d, the lateral bud former base induced medium
Are as follows: WPM+ZT 1mg/L+NAA 0.05mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4；
Culture of rootage is carried out by being forwarded in root media through lateral bud former base induction stem section of eruption lateral bud at axil, is taken root
It carries out practicing seedling after culture, cultivate Plug seedling, basin on Plug seedling.
2. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the training of taking root
Support culture 20 days, culture of rootage condition are as follows: 23 DEG C of temperature, 2 DEG C of scholar, intensity of illumination 2000-2500lx, light application time 12h/d are raw
Root culture medium are as follows: WPM+NAA 1mg/L+IBA 0.75mg/L+ sucrose 30g/L+ agar 7g/L, pH value 5.4.
3. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the experienced seedling is
Bottle seedling band bottle through culture of rootage is moved in greenhouse, under the conditions of intensity of illumination 5000-8000lx, is practiced seedling 7 days.
4. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the cultivation cave
Disk seedling is taken out bottle seedling out of bottle after experienced seedling, and after the IBA solution of the quickly dipped 8g/L in bottle seedling root, implantation fills the cave of cultivation matrix
Routinely management is cultivated to the Plug seedling of 3 side shoots of band in disk.
5. shaping methods in the bottle of alpine rose young beauty according to claim 1, which is characterized in that the Plug seedling
Upper basin is that growth forms the finished product basin seedling of 3, band hard side shoots in the flowerpot that bore is 11cm by hole tray transplantation of seedlings.
6. shaping methods in the bottle of alpine rose young beauty according to claim 4, which is characterized in that cultivated in hole tray
Matrix is turf and perlite in turf: the volume ratio of perlite is that 6:1 ratio mixes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811403140.8A CN109349109A (en) | 2018-11-23 | 2018-11-23 | Shaping methods in the bottle of alpine rose young beauty |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811403140.8A CN109349109A (en) | 2018-11-23 | 2018-11-23 | Shaping methods in the bottle of alpine rose young beauty |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109349109A true CN109349109A (en) | 2019-02-19 |
Family
ID=65338555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811403140.8A Pending CN109349109A (en) | 2018-11-23 | 2018-11-23 | Shaping methods in the bottle of alpine rose young beauty |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109349109A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113767848A (en) * | 2021-08-25 | 2021-12-10 | 山东省农业科学院 | Efficient rooting method for rhododendron lapponicum tissue culture seedlings |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003087305A2 (en) * | 2002-04-04 | 2003-10-23 | Stowers Institute For Medical Research | Methods and compositions for anchoring stem cells in a microenvironment |
| CN1568670A (en) * | 2004-04-26 | 2005-01-26 | 上海市闵行区农业科学研究所 | Tissue culture and tachytelic propagating method for alpine rhododendron |
| CN102919133A (en) * | 2012-12-03 | 2013-02-13 | 江苏省农业科学院 | Technical method for promoting tissue culture rooting of alpine rose amethyst |
| CN102919125A (en) * | 2012-11-13 | 2013-02-13 | 云南省农业科学院花卉研究所 | Method for building efficient regeneration system of Yunnan rhododendron |
| CN102960250A (en) * | 2012-11-30 | 2013-03-13 | 通化师范学院 | Method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in Changbai Mountains |
| CN103636492A (en) * | 2013-11-14 | 2014-03-19 | 石家庄市农林科学研究院 | Rhododendron hybrides cosmopolitan tissue culture rapid propagation method |
| CN104381003A (en) * | 2014-12-10 | 2015-03-04 | 湖南省森林植物园 | Cutting propagation method of rhododendron rivulare |
| CN104885773A (en) * | 2015-06-29 | 2015-09-09 | 云南省农业科学院花卉研究所 | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries |
| CN105191766A (en) * | 2015-09-02 | 2015-12-30 | 云南省农业科学院花卉研究所 | Rapid seedling hardening method for tissue culture seedlings of rhododendron lapponicum |
-
2018
- 2018-11-23 CN CN201811403140.8A patent/CN109349109A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003087305A2 (en) * | 2002-04-04 | 2003-10-23 | Stowers Institute For Medical Research | Methods and compositions for anchoring stem cells in a microenvironment |
| CN1568670A (en) * | 2004-04-26 | 2005-01-26 | 上海市闵行区农业科学研究所 | Tissue culture and tachytelic propagating method for alpine rhododendron |
| CN102919125A (en) * | 2012-11-13 | 2013-02-13 | 云南省农业科学院花卉研究所 | Method for building efficient regeneration system of Yunnan rhododendron |
| CN102960250A (en) * | 2012-11-30 | 2013-03-13 | 通化师范学院 | Method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in Changbai Mountains |
| CN102919133A (en) * | 2012-12-03 | 2013-02-13 | 江苏省农业科学院 | Technical method for promoting tissue culture rooting of alpine rose amethyst |
| CN103636492A (en) * | 2013-11-14 | 2014-03-19 | 石家庄市农林科学研究院 | Rhododendron hybrides cosmopolitan tissue culture rapid propagation method |
| CN104381003A (en) * | 2014-12-10 | 2015-03-04 | 湖南省森林植物园 | Cutting propagation method of rhododendron rivulare |
| CN104885773A (en) * | 2015-06-29 | 2015-09-09 | 云南省农业科学院花卉研究所 | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries |
| CN105191766A (en) * | 2015-09-02 | 2015-12-30 | 云南省农业科学院花卉研究所 | Rapid seedling hardening method for tissue culture seedlings of rhododendron lapponicum |
Non-Patent Citations (3)
| Title |
|---|
| R ALMEIDA等: "In vitro micropropagation of endangered Rhododendron ponticum L. subsp. baeticum (Boissier & Reuter)", 《BIODIVERSITY AND CONSERVATION》 * |
| 彭绿春等: "两种高山杜鹃试管增殖和生根影响因子", 《西部林业科学》 * |
| 杨晓琴等: "杜鹃新品种非试管智能化快繁技术", 《中国花卉园艺》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113767848A (en) * | 2021-08-25 | 2021-12-10 | 山东省农业科学院 | Efficient rooting method for rhododendron lapponicum tissue culture seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103583358B (en) | A kind of method of dendrobium cultured in vitro regeneration plant | |
| CN104585037A (en) | Tissue culture rapid-propagation method of beaucarnea recurvata | |
| CN104686353A (en) | Tissue culture technique of plumbago auriculata | |
| CN104396529A (en) | Cottage reproduction method for shrub althea flower | |
| CN104642141B (en) | Gerbera adventitious bud induction and plant regeneration method by using root as explant | |
| CN104855156A (en) | Tree shape cultivating method for Yunnan azaleas | |
| CN105340756A (en) | Method for Viburnum macrocephalum Fort. f. keteleeri in vitro culture and rapid propagation | |
| CN104782490A (en) | Establishment method of Cercis Canadensis 'Forest Pansy' tissue culture rapid propagation technical system | |
| CN104686351A (en) | In-vitro rapid propagation method of cercidiphyllum japonicum | |
| CN102217549B (en) | Culture method of rieger begonias test tube flowers | |
| CN102301950A (en) | Industrial production method of Cymbidium germchit based on sexual hybridization | |
| CN106665353B (en) | A kind of subculture method of huge cercis tissue-cultured seedling | |
| CN107027461B (en) | Method for rapidly cultivating miniature Chinese rose potted flowers by one-time pinching | |
| CN105875410B (en) | A kind of rapid propagation method of the hybrid orchid seedling of Chinese cymbidium and Cymbidium hookerianum | |
| CN107996061B (en) | Pot-covering two-section root propagation method for lily bulbs | |
| CN109349109A (en) | Shaping methods in the bottle of alpine rose young beauty | |
| CN100391333C (en) | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum | |
| CN105519445A (en) | In-vitro rapid propagation method for nepenthes | |
| CN112119915B (en) | Tissue culture rapid propagation and in vitro preservation method of alstonia-hance seedlings | |
| CN104904594B (en) | A kind of red palm pollen cultures method | |
| CN109618925B (en) | Method suitable for rapid propagation of various shrubs and herbaceous flower plants | |
| CN103621298A (en) | Cultivation method of small apple bonsai | |
| CN103931499A (en) | Tissue culture rapid propagation method for callistemon rigidus | |
| CN100409740C (en) | Medium for culturing grown-up cockscombin sealed vessel, and culture method for induction blossom | |
| CN103461136A (en) | Method for propagating crab cactus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |