CN107135758A - A kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance - Google Patents
A kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance Download PDFInfo
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- CN107135758A CN107135758A CN201710189653.2A CN201710189653A CN107135758A CN 107135758 A CN107135758 A CN 107135758A CN 201710189653 A CN201710189653 A CN 201710189653A CN 107135758 A CN107135758 A CN 107135758A
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- China
- Prior art keywords
- red palm
- putrescine
- exogenous
- seedling
- cold resistance
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance.It is 4 months, the potted plant red palm seedling in cultivation matrix by seedling age, is that red palm seedling carries out nutrition supply by supply frequency 2 times a week under greenhouse experiment, wherein, it is once 1/2Hoagland nutrient solution, another time is that 0.5mmolL is added in 1/2Hoagland‑1~2.0mmolL‑1The nutrient solution of putrescine；Nutrient solution is poured in red palm root, Exogenous Putrescine Application supplement is carried out, continues supplemented with exogenous putrescine 2 months.It is of the invention to be used for red palm cultivation, the physiological metabolism level of the red palm at low temperature can be improved, low temperature, which terminates rear blade, not to be withered, do not fall off, the energy normal growth and development in environment of 3 DEG C less than traditional cultivation temperature, it is ensured that product quality.By technical solution of the present invention, the purpose of the red palm winter production energy resource consumption of the cold resistance for improving the red palm, reduction can be reached.
Description
Technical field
The present invention relates to technical field of flower cultivation, specifically, it is related to a kind of anti-cold using the red palm of Exogenous Putrescine Application raising
The method of property.
Background technology
The red palm（Anthurium andraeanum）For Araeceae Anthurium herbaceos perennial.Because of its blade throughout the year
Evergreen, pattern is gorgeous, and viewing period is long, the important ornamental plant as winter-spring season indoor environment, turns into China's lunar New Year in recent years
One of the most salable species is spent, is also one of potted flower species salable on international market.
The red palm originates in Central and South America tropical rain forest area, and natural growing environment high temperature, how wet, Winter Cultivation low temperature can not
Less than 12 DEG C, therefore facility cultivation can only be used in the most of areas of China, annual winter heating consumption mass energy, this also into
For the key factor that red palm product price is high.Improve at present the approach of red palm cold resistance mainly by crossbreeding or
Molecular breeding means carry out, but due to China to it is red the palm germ plasm resource collection work start late and breeding cycle it is longer in terms of
The reason for, cause the red palm breed of variety of low-temperature resistance to work and relatively lag behind, fail fundamentally to reduce red palm cultivation to the energy
Rely on.Seedling stage cultivation addition allogenic material is an advantage of lower cost, the approach for the comparatively faster raising plant resistance to environment stress that takes effect.
The Exogenous Putrescine Application for adding suitable concentration currently with nutrient solution cultivates the red palm, so that the mature technology for improving red palm cold resistance has no
Report.
The content of the invention
The present invention can effectively improve the red palm low for the red deficiency for slapping breed of variety presence of existing low-temperature resistance there is provided one kind
Physiological metabolism level under the conditions of temperature, makes red cultural method of the palm with cold resistance.
For achieving the above object, the technical solution adopted by the present invention is to provide one kind using the red palm of Exogenous Putrescine Application raising
The method of cold resistance, is 4 months, the potted plant red palm seedling in cultivation matrix by seedling age, is 25~28 DEG C of daytime in growing temperatures,
It is red palm seedling nutrition supply by supply frequency 2 times a week under 18~21 DEG C of greenhouse experiment of night, wherein, it is once 1/
2Hoagland nutrient solution, another time is to add 0.5mmolL in 1/2Hoagland nutrient solutions-1~2.0mmolL-1
Putrescine；Nutrient solution is poured in red palm root, Exogenous Putrescine Application supplement is carried out, continues supplemented with exogenous putrescine 2 months；After 2 months, press
1 times a week, 2/3 Hoagland nutrient solution nutrition supplies.
The present invention a preferred scheme be：1.0mmolL is added in 1/2Hoagland nutrient solutions-1Putrescine.The red palm
Seedling is carried out potted plant with a diameter of 9cm flowerpot；By weight, cultivation matrix is 3 parts of coconut husk blocks:2 parts of potted plant peat:1 part of pearl
Rock.
Described putrescine is the pure putrescine of analysis.
The method provided by the present invention, can improve the physiological metabolism level of the red palm at low temperature, and low temperature terminates rear blade not
Wither, do not fall off, the energy normal growth and development in environment of 3 DEG C less than traditional cultivation temperature, it is ensured that product quality.
Compared with prior art, the method that the present invention improves red palm cold resistance using Exogenous Putrescine Application, the greenhouse for the red palm
Cultivation, can make plant be amenable to not be shorter than 3d 6 DEG C of consecutive low temperature, and temperature recovers rear blade without withered or obscission, energy
Continue normal growth under conditions of 3 DEG C of subnormal temperature and bloom.The present invention adds suitable concentration using nutrition supply
The method of Exogenous Putrescine Application, improves the cold resistance of the red palm under cryogenic, and can make the red palm less than normal 3 DEG C of growing temperatures
In the environment of normal growth and development, and then reach reduce it is red the palm winter production energy resource consumption purpose, for the low of flower crop
Energy consumption is produced and reduction product price has realistic meaning.
Embodiment
With reference to specific embodiment, technical solution of the present invention is further elaborated.
Embodiment 1
The present embodiment provides a kind of to improve red palm cold resistance, using the method for Exogenous Putrescine Application.
The red palm seedling age that the present embodiment is used, for 4 months, is carried out potted plant with a diameter of 9cm flowerpot；By weight, plant
It is 3 parts of coconut husk blocks to train matrix:2 parts of potted plant peat:1 part of perlite.The nutrition supply frequency of red palm seedling for 2 times a week, once for
1/2Hoagland nutrient solutions, once to add 0.5mmolL in 1/2Hoagland nutrient solutions-1Exogenous Putrescine Application.Exogenous Putrescine Application
For AR, the root that red palm seedling is poured in the nutrient solution diluted is mixed into after precise.Add Exogenous Putrescine Application when
Between be 2 months, 2 months after nutrition supply switch to 1 times a week, 2/3 Hoagland nutrient solutions.Cultivated under greenhouse experiment, in vain
It 26 DEG C, night, 20 DEG C of greenhouses had full-automatic temperature-controlling, scavenging utilities, ensure that and provided one during testing for material to be tested
Individual metastable suitable growing environment.
By above-mentioned technical proposal, 1.0,1.5 and 2.0mmolL are added respectively in 1/2Hoagland nutrient solutions-1It is outer
Source putrescine carries out the administration of the Exogenous Putrescine Application of red palm seedling.
Embodiment 2
By the technical scheme of embodiment 1,0.5,1.0,1.5 and 2.0mmolL is added in 1/2Hoagland nutrient solutions respectively-1
Exogenous Putrescine Application, to red palm seedling carry out Exogenous Putrescine Application administration, observation different disposal method under low temperature it is red the palm physical signs and
The influence of nitrogen metabolism.The present embodiment to be not added with the red palm seedling of Exogenous Putrescine Application as control, with addition 0.5,1.0,1.5,
2.0mmol·L-1Exogenous Putrescine Application cultivate the red palm seedling of 2 months and be together put into 6 DEG C of low temperature, intensity of illumination 6000lux, RH85%,
In light application time 12h/d incubator after processing 3d, take red palm root system and blade to carry out physiological index determining immediately, planted with normal temperature
Training, CK（Low temperature）Contrast, the influence to red palm Antioxidant enzymes activity and root activity after 6 DEG C of low temperature stress 3d, its result
Referring to table 1；Influence to red palm leaf chlorophyll, MDA and free proline content after 6 DEG C of low temperature stress 3d, its result ginseng
It is shown in Table 2；Influence to red palm leaves N-metabolism after 6 DEG C of low temperature stress 3d, its result is referring to table 3.
Influence of the different disposal of table 1 to red palm Antioxidant enzymes activity and root activity after 6 DEG C of low temperature stress 3d
。
The different disposal of table 2 is to red palm leaf chlorophyll after 6 DEG C of low temperature stress 3d, MDA and free proline content
Influence
。
Influence of the different disposal of table 3 to red palm leaves N-metabolism after 6 DEG C of low temperature stress 3d
。
The POD activity of the red palm, SOD activity, CAT activity and root system activity after low-temperature treatment it can be seen from 1~table of table 3
It is above the processing of normal temperature cultivation, chlorophyll content reduction, the increase of the osmotic adjustment such as MDA and Proline, nitrogen
Metaboilic level is raised, and shows the red palm seedling low temperature stress extraneous by adjusting the physiological metabolism active response of itself.With CK（Do not add
Plus Exogenous Putrescine Application）Compare, the processing of addition Exogenous Putrescine Application has higher activities of antioxidant enzymes and root activity；Leaf chlorophyll
Content is higher, and Lipid peroxidation metabolism degree is lower, and structure is more stable；High nitrogen metabolism level relatively can be maintained.Show red palm seedling
Phase cultivation addition 0.5mmolL-1~2.0mmolL-1Exogenous Putrescine Application, especially 1.0mmolL-1Exogenous Putrescine Application for
The cold resistance for improving the red palm serves key effect.
Embodiment 3
By the technical scheme of embodiment 1,0.5,1.0,1.5 and 2.0mmolL is added in 1/2Hoagland nutrient solutions respectively-1's
Exogenous Putrescine Application carries out the administration of the Exogenous Putrescine Application of red palm seedling, and the present embodiment provides the red palm seedling restoration ecosystem of different disposal method
Appearance, as a result referring to table 4.
The appearance of the red palm seedling restoration ecosystem of table 4
。
It can be seen from Table 4 that, the red palm seedling of addition Exogenous Putrescine Application recovers after low temperature terminates preferably, through the observation of 4 weeks
Record does not occur the phenomenon that blade is withered and comes off.Though be not added with the red palm seedling of Exogenous Putrescine Application at the end of low temperature it is not apparent go out
Obvious damage symptoms, but show during the restoration ecosystem in later stage that obvious blade is withered and obscission, show pair
According to red palm seedling miss to adapt to the low-temperature treatment intensity that sets of the present invention.
Embodiment 4
By the technical scheme of embodiment 1,0.5,1.0,1.5 and 2.0mmolL is added in 1/2Hoagland nutrient solutions respectively-1's
Exogenous Putrescine Application carries out the administration of the Exogenous Putrescine Application of red palm seedling, and the present embodiment provides the hair of the red palm seedling to addition Exogenous Putrescine Application cultivation
Situation result is educated, referring to table 5.
The development condition of the red palm seedling of the addition Exogenous Putrescine Application cultivation of table 5
| Processing method | Moon number needed for blooming（It is individual） | Average flower diameter（cm） | Average individual plant flower number（Piece） |
| Normal temperature is cultivated | 7 | 6.5 | 3 |
| CK（Low temperature） | 8.5 | 6.2 | 3 |
| The mmolL of low temperature+put 0.5-1 | 7 | 6.6 | 3 |
| The mmolL of low temperature+put 1.0-1 | 7 | 6.7 | 3 |
| The mmolL of low temperature+put 1.5-1 | 7 | 6.7 | 3 |
| The mmolL of low temperature+put 2.0-1 | 7 | 6.6 | 3 |
It can be seen from Table 5 that, the red palm seedling of addition Exogenous Putrescine Application can normally be sent out in the environment less than normal 3 DEG C of growing temperatures
Educate, the time required to blooming, average flower diameter and average individual plant flower number with the no significant difference under normal growing temperatures.Not
The red palm seedling of addition Exogenous Putrescine Application is bloomed delay in the environment less than normal 3 DEG C of growing temperatures, and flower diminishes.Show addition
0.5mmol·L-1~2.0mmolL-1Exogenous Putrescine Application can guarantee that the cultivation effect of red palm during less than 3 DEG C of normal growing temperatures,
And with 1.0mmolL-1Exogenous Putrescine Application best results.
Claims (4)
1. a kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance, it is characterised in that：By seedling age be 4 months, it is potted plant in cultivation
The red palm seedling in matrix is trained, in the case where growing temperatures are 25~28 DEG C of daytime, 18~21 DEG C of greenhouse experiment of night, by 2 times a week
Supply frequency be red palm seedling nutrition supply, wherein, be once 1/2Hoagland nutrient solution, another time is 1/
0.5mmolL is added in 2Hoagland nutrient solutions-1~2.0mmolL-1Putrescine；Nutrient solution is poured in red palm root, entered
Row Exogenous Putrescine Application is supplemented, and continues supplemented with exogenous putrescine 2 months；After 2 months, by 1 times a week, sought with 2/3 Hoagland nutrient solutions
Support supply.
2. the method that a kind of utilization Exogenous Putrescine Application according to claim 1 improves red palm cold resistance, it is characterised in that：1/
1.0mmolL is added in 2Hoagland nutrient solutions-1Putrescine.
3. the method that a kind of utilization Exogenous Putrescine Application according to claim 1 improves red palm cold resistance, it is characterised in that：The red palm
Seedling is carried out potted plant with a diameter of 9cm flowerpot；By weight, cultivation matrix is 3 parts of coconut husk blocks:2 parts of potted plant peat:1 part of pearl
Rock.
4. the method that a kind of utilization Exogenous Putrescine Application according to claim 1 improves red palm cold resistance, it is characterised in that：It is described
Putrescine to analyze pure putrescine.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710189653.2A CN107135758A (en) | 2017-03-27 | 2017-03-27 | A kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710189653.2A CN107135758A (en) | 2017-03-27 | 2017-03-27 | A kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance |
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| CN107135758A true CN107135758A (en) | 2017-09-08 |
Family
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000290102A (en) * | 1999-03-31 | 2000-10-17 | Toyobo Co Ltd | Method for enhancing low temperature stress tolerance of plant |
| CN101637129A (en) * | 2009-08-25 | 2010-02-03 | 浙江大学 | Culture method of cold-resistant anthurium andraenum |
| CN102651969A (en) * | 2009-12-11 | 2012-08-29 | 住友化学株式会社 | Method for reducing temperature stress of plants |
| US20130180173A1 (en) * | 2011-12-21 | 2013-07-18 | E I Du Pont De Nemours And Company | Plant artificial seeds having multilayers and methods for the production thereof |
| CN105210701A (en) * | 2015-10-28 | 2016-01-06 | 上海交通大学 | The method at Chinese narcissus florescence is regulated with Spd |
| CN106490052A (en) * | 2016-09-12 | 2017-03-15 | 浙江大学 | Compound spray and its application in promotion crop kernel grouting and seed vitality |
-
2017
- 2017-03-27 CN CN201710189653.2A patent/CN107135758A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000290102A (en) * | 1999-03-31 | 2000-10-17 | Toyobo Co Ltd | Method for enhancing low temperature stress tolerance of plant |
| CN101637129A (en) * | 2009-08-25 | 2010-02-03 | 浙江大学 | Culture method of cold-resistant anthurium andraenum |
| CN102651969A (en) * | 2009-12-11 | 2012-08-29 | 住友化学株式会社 | Method for reducing temperature stress of plants |
| US20130180173A1 (en) * | 2011-12-21 | 2013-07-18 | E I Du Pont De Nemours And Company | Plant artificial seeds having multilayers and methods for the production thereof |
| CN105210701A (en) * | 2015-10-28 | 2016-01-06 | 上海交通大学 | The method at Chinese narcissus florescence is regulated with Spd |
| CN106490052A (en) * | 2016-09-12 | 2017-03-15 | 浙江大学 | Compound spray and its application in promotion crop kernel grouting and seed vitality |
Non-Patent Citations (1)
| Title |
|---|
| 田丹青，葛亚英，潘刚敏，等: "不同外源物质处理对红掌抗寒性的影响", 《浙江农业科学》 * |
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Application publication date: 20170908 |