CN103392594A - Draceana arborea tissue culture seedling rooting induction technology - Google Patents
Draceana arborea tissue culture seedling rooting induction technology Download PDFInfo
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- CN103392594A CN103392594A CN2013102731384A CN201310273138A CN103392594A CN 103392594 A CN103392594 A CN 103392594A CN 2013102731384 A CN2013102731384 A CN 2013102731384A CN 201310273138 A CN201310273138 A CN 201310273138A CN 103392594 A CN103392594 A CN 103392594A
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Abstract
The invention discloses a draceana arborea tissue culture seedling rooting induction technology. Compared with the prior art, the rooting induction and culture technology of the draceana arborea tissue culture seedling has advantages of short rooting time, high rooting rate and good growth of rooted seedlings, and can provide better technology support for industrialization production of draceana arborea.
Description
Technical field
The present invention relates to a kind of root induction technology, relate in particular to the group training seedling rooting inductive technology of golden heart Yemen iron.
Background technology
Gold heart Yemen's iron (Draceana arborea) claims golden heart Brazil iron not only but also cries Brazil wood in thousand, the horticultural variety of fragrant dragon tree, for the Agavaceae dracaena plant, its shape leaf consor stem top, broadband, there is a golden yellow wide striped in blade central authorities, the both sides green is a kind of good ornamental foliage plant.Because of extremely anti-the moon, evergreen all the year round, simple and convenient management, growth is slow, the life-span is long, is placed in the general place of indoor light and can puts 6 months to 2 years, effectively the pernicious gases such as the interior formaldehyde of adsorption chamber, benzene, become Chinese important Ornamental Foliage House Plants.Gold heart Yemen's ornamental work of iron phase is long, and blossom and bear fruit and reach the several years, and few branch, therefore use seminal propagation and cottage propagation to be difficult to meet Production requirement, it is grown seedlings on a large scale and can only be undertaken by organizing the cultivation approach.
About the report of the Study on tissue culture of golden heart Yemen iron seldom, and be all the research of setting up regenerating system in laboratory, there are no specially to the research report of group training seedling rooting inductive technology.The object of the invention is to carry out series of studies by the condition of culture to golden heart Yemen iron group training seedling rooting, draw the condition of culture that is suitable for golden heart Yemen iron group training seedling rooting, for the industrialization production of golden heart Yemen iron provides technical support.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of golden heart Yemen iron group training seedling rooting inductive technology be provided, comprising:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.0-1.5mg/L;
Surplus is distilled water.
In the present invention, the compound method of golden heart Yemen iron group training seedling rooting medium is:
The preparation of 1 mother liquor and preservation
1.1 for ease of sampling, should first prepare various mother liquors, be divided into macroelement mother liquor, micro-mother liquor, organic matter mother liquor and each plant growth regulators mother liquor.Sucrose, agar should not be made into mother liquor, while needing, directly claim sample;
1.2 the macroelement mother liquid concentration becomes 100 times of solution, organic matter, micro-mother liquor are made into 200 times of solution, and the concentration of plant-growing-help chemicals mother liquor is made into 1mg/ml;
1.3 mother liquor is selected aseptic distilled water, deionized water or ultra-pure water preparation, while producing in a large number, uses the water that boiled;
1.4 during preparation macroelement mother liquor, each component should be dissolved separately, by the order of nitrogen, calcium, magnesium, phosphorus, mixes one by one afterwards, otherwise easily causes precipitation;
1.5 trace element, organic matter, plant growth regulator mother liquor application brown bottle install and are placed in refrigerator and preserve;
1.6 after the mother liquor preparation, should in time use, period of storage should not be over 1 month;
1.7 find that mother liquor has precipitation, or growth of microorganism arranged, or algal grown is arranged, should pass into disuse.
The preparation of 2 medium
2.1, according to culture medium prescription, measure in proportion various mother liquors;
2.2 in dosing container, put into the approximately pure water more than 1/2 of preparing preparation medium total amount, and add appropriate sugar, then add one by one while stirring the mother liquor of aequum;
But 2.3 add after the agar heat fused, during if any mixing plant, also can directly add agar powder, the total amount of then supplying required preparation medium with pure water, stir and get final product;
2.4 the sugar in medium, in a large amount of group training seedlings are produced, general available commercially available white sugar, but garden beet sugar with sucrose and not preferably;
2.5 with the sodium hydroxide of the hydrochloric acid of 1.0 mol/L or 1.0 mol/L, regulate the pH value to 5.8 of medium;
2.6 the medium for preparing will divide and install in culture vessel as early as possible, in order to avoid culture medium solidifying or retrogradation and be difficult to packing;
2.7 during the packing medium, should note avoiding medium to be bonded on bottleneck, if be stained with medium, before lid bottle cap or bottle stopper, must clean bottleneck with clean gauze.
3 medium sterilizations
3.1 will divide the medium that installs to be loaded on sterilization in high-pressure sterilizing pot;
3.2 the heating initial stage, when the air pressure of sterilizing pan disinfection room reaches 0.05Mpa, is opened condensation trap, drains cold air in disinfection room;
3.3, when the disinfection room internal gas pressure reaches 0.11Mpa, when temperature reaches 121 ℃, starts timing, keep temperature and pressure sterilization 15-20 minute;
3.4 close the heating power supply switch, by slow exhaust mode discharging hot gas, when being down to atmospheric pressure, opens indoor air pressure to be sterilized sterilization pot cover or door, take out medium;
3.5 medium should be placed in, and little air flows and the clean environment of few dust is cooling, otherwise causes mycotic spore to enter culture vessel in the cooling intake process, produces mould contamination.
4 medium storages
4.1 medium should be as far as possible now with the current;
4.2 can be at air clean and immobilising environment short time storage medium, but storage surpasses the medium of 1 month, should pass into disuse.
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 2000-3000lx, temperature are to cultivate about 10 days under the condition of 25-28 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is sturdy, and seedling leaf look dark green, and internode extends gradually, until grow up to complete plantlet.
Compared with prior art, advantage is the golden heart of the present invention Yemen iron group training seedling rooting inductive technology:
1, root media difference is higher than the rooting rate of existing root media;
2, condition of culture difference: prior art is that group training is taken root after seedling is inoculated into root media and is placed under low light condition or the complete dark lower 7-10 days of cultivation, then is placed on and cultivates than under high light, needs like this about 25 days just to start root; And the present invention utilizes the impact of illuminance on golden heart Yemen iron group training seedling rooting, finding out the optimal light illumination that is suitable for golden heart Yemen iron group training seedling rooting is 2000-3000lx, by after the inoculation of group training seedling rooting, directly being placed under the illumination condition of 2000-3000lx, cultivates.Only need to cultivate 10 days left and right seedlings and just start root;
3, adopt of the present invention taking root to have the method rooting rate of cultivating than the first low light level generally to technology high, rooting rate reaches 98.0%, goes out the root time faster than prior art, the growth of seedling of taking root is also better, well developed root system, and on average going out the root amount has the 3-5 bar, can transplant in 30 days, transplanting survival rate reaches 98%.
Embodiment:
Below the invention will be further described with embodiment, but the present invention is not limited to these embodiment.
Embodiment 1:
The inductive technology of a kind of golden heart Yemen iron group training seedling rooting comprises:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.0mg/L;
Surplus is distilled water, prepares, and is stand-by;
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 2000lx, temperature are to cultivate 10 days under the condition of 26 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is sturdy, and seedling leaf look dark green, and internode extends gradually, until grow up to complete plantlet, rooting rate reaches 98.2%.
Embodiment 2:
The inductive technology of a kind of golden heart Yemen iron group training seedling rooting comprises:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.5mg/L;
Surplus is distilled water, prepares, and is stand-by;
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 2500lx, temperature are to cultivate 10 days under the condition of 28 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is sturdy, and seedling leaf look dark green, and internode extends gradually, until grow up to complete plantlet, rooting rate reaches 98.5%.
Embodiment 3:
The inductive technology of a kind of golden heart Yemen iron group training seedling rooting comprises:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.0mg/L;
Surplus is distilled water, prepares, and is stand-by;
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 3000lx, temperature are to cultivate 10 days under the condition of 25 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is sturdy, and seedling leaf look dark green, and internode extends gradually, until grow up to complete plantlet, rooting rate reaches 98.0%.
Comparative Examples 1:
The inductive technology of a kind of golden heart Yemen iron group training seedling rooting comprises:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.0-1.5mg/L;
Surplus is distilled water, prepares, and is stand-by;
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 0lx, temperature are to cultivate 25 days under the condition of 26 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is tiny, seedling leaf look pale green, and the seedling internode of taking root has no obvious elongation, and rooting rate is 20.0%.
Comparative Examples 2:
The inductive technology of a kind of golden heart Yemen iron group training seedling rooting comprises:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.0mg/L;
Surplus is distilled water, prepares, and is stand-by;
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 200lx, temperature are to cultivate 25 days under the condition of 26 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is tiny, seedling leaf look pale green, and the seedling internode of taking root has no obvious elongation, and rooting rate is 46.0%.
Comparative Examples 3:
The inductive technology of a kind of golden heart Yemen iron group training seedling rooting comprises:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.0mg/L;
Surplus is distilled water, prepares, and is stand-by;
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 500lx, temperature are to cultivate 25 days under the condition of 26 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is tiny, seedling leaf look pale green, and the seedling internode of taking root has no obvious elongation, and rooting rate is 62.0%.
Utilize medium and the condition of culture of above-described embodiment and Comparative Examples, golden heart Yemen iron group training seedling is carried out to root induction, from the result of following table, can find out, the technical indicator of embodiment far surpasses Comparative Examples.
Claims (1)
1. the inductive technology of a golden heart Yemen iron group training seedling rooting is characterized in that:
A, group training seedling rooting medium, wherein, the component of every liter of (L) root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 950mg/L, ammonium nitrate 825mg/L, calcium chloride dihydrate 200mg/L, epsom salt 185mg/L, potassium dihydrogen phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate heptahydrate 27.5mg/L;
(2) trace element: four water manganese sulphate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, potassium iodide 0.83mg/L, Sodium Molybdate Dihydrate 0.25mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L;
(3) organic matter: inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride (VB
1) 0.1mg/L, puridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L;
(4) plant growth regulator: indolebutyric acid 1.0-1.5mg/L;
Surplus is distilled water, prepares, and is stand-by;
B, inducing of taking root
From golden heart Yemen iron group training subculture seedling, cutting the bud seedling more than more healthy and stronger, high 2cm, be seeded to root induction on root media, after inoculation is good, places, cultivate;
The cultivation of c, the seedling of taking root
It is that 2000-3000lx, temperature are to cultivate about 10 days under the condition of 25-28 ℃ that the seedling of taking root of having inoculated is placed on illuminance, and seedling just starts root, and root is sturdy, and seedling leaf look dark green, and internode extends gradually, until grow up to complete plantlet.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221689A (en) * | 2014-09-29 | 2014-12-24 | 钦州市林业科学研究所 | Potting technology for Dracaena arborea |
| CN104478580A (en) * | 2014-12-08 | 2015-04-01 | 镇江市官塘生态农业有限公司 | Rose cuttings rooting solution and preparation method thereof |
| CN108112476A (en) * | 2017-12-19 | 2018-06-05 | 佛山市三水阳特园艺有限公司 | A kind of Jin Xin Yemen iron quick breeding method for tissue culture |
-
2013
- 2013-07-02 CN CN2013102731384A patent/CN103392594A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 易清等: "3 个也门铁品种高效离体繁殖体系的建立", 《湖南农业大学学报（自然科学版）》 * |
| 柯碧英等: "金心也门铁组培快速繁殖技术研究", 《广东农业科学》 * |
| 王方正等: "也门铁的组织培养与快速繁殖", 《植物生理学通讯》 * |
| 葛胜娟: "《植物组织培养》", 30 September 2008, 中国农业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221689A (en) * | 2014-09-29 | 2014-12-24 | 钦州市林业科学研究所 | Potting technology for Dracaena arborea |
| CN104478580A (en) * | 2014-12-08 | 2015-04-01 | 镇江市官塘生态农业有限公司 | Rose cuttings rooting solution and preparation method thereof |
| CN108112476A (en) * | 2017-12-19 | 2018-06-05 | 佛山市三水阳特园艺有限公司 | A kind of Jin Xin Yemen iron quick breeding method for tissue culture |
| CN108112476B (en) * | 2017-12-19 | 2019-01-01 | 佛山市三水阳特园艺有限公司 | A kind of Jin Xin Yemen iron quick breeding method for tissue culture |
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Application publication date: 20131120 |