CN205018021U - Plant tissue culture bottle lid of adjustable gas permeability - Google Patents
Plant tissue culture bottle lid of adjustable gas permeability Download PDFInfo
- Publication number
- CN205018021U CN205018021U CN201520607834.9U CN201520607834U CN205018021U CN 205018021 U CN205018021 U CN 205018021U CN 201520607834 U CN201520607834 U CN 201520607834U CN 205018021 U CN205018021 U CN 205018021U
- Authority
- CN
- China
- Prior art keywords
- bottle cap
- bottle lid
- lower floor
- upper strata
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 16
- 230000035699 permeability Effects 0.000 title claims abstract description 14
- 230000012010 growth Effects 0.000 abstract description 5
- 230000003014 reinforcing effect Effects 0.000 abstract 1
- 230000029058 respiratory gaseous exchange Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 3
- 239000005418 vegetable material Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000009569 heterotrophic growth Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The utility model relates to a plant tissue culture bottle lid of adjustable gas permeability belongs to the plant tissue culture field. The utility model discloses an upper strata bottle lid, lower floor's bottle lid, bleeder vent, arc -shaped strip describe the opening, the upper strata is equipped with arc -shaped strip form opening on the bottle lid, is equipped with the bleeder vent on lower floor's bottle lid of corresponding with arc -shaped strip form opening, and upper bottle lid rotates on lower floor's bottle lid at will, and lower floor's bottle lid is turned round tightly at the blake bottle bottleneck. The utility model discloses an upper bottle lid of rotation is adjusted the contact ratio and overlap ratio of upper bottle lid opening and the aseptic bleeder vent of lower floor's bottle lid and is adjusted the bottle lid volume of breathing freely, and the gas inside and outside the reinforcing culture vessel exchanges, reduces the hygrometry in the container, and the growth of banking up the seedling with earth is organized in the promotion, and the quality of banking up the seedling with earth is organized in the improvement.
Description
Technical field
The utility model relates to a kind of plant tissue culture bottle lid of adjustable gas permeability, belongs to field of plant tissue culture.
Background technology
Traditional Plant Tissue Breeding mode, for preventing pollution adopts the container that seal is very strong, under certain illumination condition, with certain light and shade cycle constant temperature culture.Plantlet in vitro carries out heterotrophic growth, because container humidity is higher, gas fluidity is poor, CO by the sugar in medium
2not enough, secondary metabolism toxic substance concentration is higher, thus directly causes in plantlet in vitro form and physiological variation, occurs vitreous shoot and environmental suitability difference etc. to external world after emerging as poor growth, easily; And higher relative moisture can cause the structure of test-tube plantlet blade to change, transpiration pull reduces, and have impact on the normal growth of plantlet in vitro; Microbial contamination is simultaneously serious.
Summary of the invention
The deficiencies such as the utility model provides a kind of plant tissue culture bottle lid of adjustable gas permeability, higher for the existing container humidity of solution, gas fluidity is poor.
The technical solution of the utility model is: a kind of plant tissue culture bottle lid of adjustable gas permeability, comprises upper strata bottle cap, lower floor's bottle cap, air-vent 1, arc strip opening 3; Described upper strata bottle cap is provided with arc strip opening 3, and the lower floor bottle cap corresponding with arc strip opening 3 is provided with air-vent 1, and upper strata bottle cap arbitrarily rotates on lower floor's bottle cap, and lower floor's bottle cap tightens at blake bottle bottleneck.
Also comprise convex track I 2, convex track II 4, cylindrical projections 5; Described convex track II 4 is positioned at upper strata bottle cap, the cylindrical projections 5 less than upper strata bottle cap is provided with above lower floor's bottle cap, the outer wall of cylindrical projections 5 is provided with convex track I 2, convex track II 4 is stuck in convex track I 2 times, make two track tight connectings, upper strata bottle cap is arbitrarily rotated on lower floor's bottle cap.
The arc length of described arc strip opening 3 is the right arc length of the central angle of 90 °.
The course of work of the present utility model is:
The medium prepared is loaded in plant tissue culture bottle with described adjustable gas permeability cultivation bottle cap, then the convex track II 4 of upper strata bottle cap inwall is snapped into the convex track I 2 times of lower floor's bottle cap cylindrical projections 5 outer wall, again lower floor's bottle cap is tightened and put into high-pressure sterilizing pot at bottleneck and carry out sterilizing, then the blake bottle that medium is housed that sterilizing cools is moved on on superclean bench and connect vegetable material.The blake bottle connecting vegetable material is put into culturing room.In incubation, according to different vegetable material at different times to CO
2the requirement of concentration and humidity, rotates upper strata bottle cap, regulates the registration of the air-vent 1 on upper strata bottle cap two 90 degree arc strip openings 3 and lower floor's bottle cap, regulates Air permenbility, meets plantlet in vitro at different growing stage to CO
2and the requirement of humidity.Thus promote the quick growth of plantlet in vitro, avoid a bottle vitrification phenomenon for implants material, improve the quality of plantlet in vitro, finally reach the object of the plantlet in vitro obtaining high-quality fast.
The beneficial effects of the utility model are: by rotating upper layer bottle cap, the registration of upper strata bottle cap opening and the aseptic air-vent of lower floor's bottle cap is regulated to regulate bottle cap Air permenbility, strengthen the relative moisture in the gas exchanges inside and outside culture vessel, reduction container, promote the growth of plantlet in vitro, improve the quality of plantlet in vitro.
Accompanying drawing explanation
Fig. 1 is the vertical view of lower floor's bottle cap in the utility model;
Fig. 2 is the stereogram of lower floor's bottle cap in the utility model;
Fig. 3 is the front view of lower floor's bottle cap in the utility model;
Fig. 4 is the vertical view of the utility model bottle cap at the middle and upper levels;
Fig. 5 is the stereogram of the utility model bottle cap at the middle and upper levels;
Fig. 6 be the utility model at the middle and upper levels bottle cap cover on lower floor's bottle cap after vertical view;
Each label in figure: 1-air-vent, 2-convex track I, 3-arc strip opening, 4-convex track II, 5-cylindrical projections.
Embodiment
Embodiment 1: as shown in figures 1 to 6, a kind of plant tissue culture bottle lid of adjustable gas permeability, comprises upper strata bottle cap, lower floor's bottle cap, air-vent 1, arc strip opening 3; Described upper strata bottle cap is provided with arc strip opening 3, and the lower floor bottle cap corresponding with arc strip opening 3 is provided with air-vent 1, and upper strata bottle cap arbitrarily rotates on lower floor's bottle cap, and lower floor's bottle cap tightens at blake bottle bottleneck.
Also comprise convex track I 2, convex track II 4, cylindrical projections 5; Described convex track II 4 is positioned at upper strata bottle cap, the cylindrical projections 5 less than upper strata bottle cap is provided with above lower floor's bottle cap, the outer wall of cylindrical projections 5 is provided with convex track I 2, convex track II 4 is stuck in convex track I 2 times, make two track tight connectings, upper strata bottle cap is arbitrarily rotated on lower floor's bottle cap.
The arc length of described arc strip opening 3 is the right arc length of the central angle of 90 °.
Embodiment 2: as shown in figures 1 to 6, a kind of plant tissue culture bottle lid of adjustable gas permeability, comprises upper strata bottle cap, lower floor's bottle cap, air-vent 1, arc strip opening 3; Described upper strata bottle cap is provided with arc strip opening 3, and the lower floor bottle cap corresponding with arc strip opening 3 is provided with air-vent 1, and upper strata bottle cap arbitrarily rotates on lower floor's bottle cap, and lower floor's bottle cap tightens at blake bottle bottleneck.
Also comprise convex track I 2, convex track II 4, cylindrical projections 5; Described convex track II 4 is positioned at upper strata bottle cap, the cylindrical projections 5 less than upper strata bottle cap is provided with above lower floor's bottle cap, the outer wall of cylindrical projections 5 is provided with convex track I 2, convex track II 4 is stuck in convex track I 2 times, make two track tight connectings, upper strata bottle cap is arbitrarily rotated on lower floor's bottle cap.
Embodiment 3: as shown in figures 1 to 6, a kind of plant tissue culture bottle lid of adjustable gas permeability, comprises upper strata bottle cap, lower floor's bottle cap, air-vent 1, arc strip opening 3; Described upper strata bottle cap is provided with arc strip opening 3, and the lower floor bottle cap corresponding with arc strip opening 3 is provided with air-vent 1, and upper strata bottle cap arbitrarily rotates on lower floor's bottle cap, and lower floor's bottle cap tightens at blake bottle bottleneck.
The arc length of described arc strip opening 3 is the right arc length of the central angle of 90 °.
Embodiment 4: as shown in figures 1 to 6, a kind of plant tissue culture bottle lid of adjustable gas permeability, comprises upper strata bottle cap, lower floor's bottle cap, air-vent 1, arc strip opening 3; Described upper strata bottle cap is provided with arc strip opening 3, and the lower floor bottle cap corresponding with arc strip opening 3 is provided with air-vent 1, and upper strata bottle cap arbitrarily rotates on lower floor's bottle cap, and lower floor's bottle cap tightens at blake bottle bottleneck.
By reference to the accompanying drawings embodiment of the present utility model is explained in detail above, but the utility model is not limited to above-mentioned embodiment, in the ken that those of ordinary skill in the art possess, various change can also be made under the prerequisite not departing from the utility model aim.
Claims (3)
1. a plant tissue culture bottle lid for adjustable gas permeability, is characterized in that: comprise upper strata bottle cap, lower floor's bottle cap, air-vent (1), arc strip opening (3); Described upper strata bottle cap is provided with arc strip opening (3), and the lower floor bottle cap corresponding with arc strip opening (3) is provided with air-vent (1), and upper strata bottle cap arbitrarily rotates on lower floor's bottle cap, and lower floor's bottle cap tightens at blake bottle bottleneck.
2. the plant tissue culture bottle lid of adjustable gas permeability according to claim 1, is characterized in that: also comprise convex track I (2), convex track II (4), cylindrical projections (5); Described convex track II (4) is positioned at upper strata bottle cap, the cylindrical projections (5) less than upper strata bottle cap is provided with above lower floor's bottle cap, the outer wall of cylindrical projections (5) is provided with convex track I (2), under convex track II (4) is stuck in convex track I (2), make two track tight connectings, upper strata bottle cap is arbitrarily rotated on lower floor's bottle cap.
3. the plant tissue culture bottle lid of adjustable gas permeability according to claim 1 and 2, is characterized in that: the arc length of described arc strip opening (3) is the right arc length of the central angle of 90 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520607834.9U CN205018021U (en) | 2015-08-13 | 2015-08-13 | Plant tissue culture bottle lid of adjustable gas permeability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520607834.9U CN205018021U (en) | 2015-08-13 | 2015-08-13 | Plant tissue culture bottle lid of adjustable gas permeability |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205018021U true CN205018021U (en) | 2016-02-10 |
Family
ID=55250603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520607834.9U Expired - Fee Related CN205018021U (en) | 2015-08-13 | 2015-08-13 | Plant tissue culture bottle lid of adjustable gas permeability |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205018021U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410701A (en) * | 2018-06-01 | 2018-08-17 | 新洲(武平)林化有限公司 | A kind of microbial method preparation high-purity terpinol shake culture device |
| CN110122095A (en) * | 2019-06-21 | 2019-08-16 | 中国农业科学院棉花研究所 | A kind of dual-purpose nutrition of cotton training tissue culture seedling grafting covers alms bowl and its application method |
-
2015
- 2015-08-13 CN CN201520607834.9U patent/CN205018021U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410701A (en) * | 2018-06-01 | 2018-08-17 | 新洲(武平)林化有限公司 | A kind of microbial method preparation high-purity terpinol shake culture device |
| CN110122095A (en) * | 2019-06-21 | 2019-08-16 | 中国农业科学院棉花研究所 | A kind of dual-purpose nutrition of cotton training tissue culture seedling grafting covers alms bowl and its application method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160210 Termination date: 20170813 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |