CN110122095A - A kind of dual-purpose nutrition of cotton training tissue culture seedling grafting covers alms bowl and its application method - Google Patents

A kind of dual-purpose nutrition of cotton training tissue culture seedling grafting covers alms bowl and its application method Download PDF

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Publication number
CN110122095A
CN110122095A CN201910542112.2A CN201910542112A CN110122095A CN 110122095 A CN110122095 A CN 110122095A CN 201910542112 A CN201910542112 A CN 201910542112A CN 110122095 A CN110122095 A CN 110122095A
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CN
China
Prior art keywords
alms bowl
seedling
housing
dual
trophosome
Prior art date
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Pending
Application number
CN201910542112.2A
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Chinese (zh)
Inventor
李燕
张永山
陈伟
姚金波
房圣涛
李秋琳
李夏轩
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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Application filed by Institute of Cotton Research of Chinese Academy of Agricultural Sciences filed Critical Institute of Cotton Research of Chinese Academy of Agricultural Sciences
Priority to CN201910542112.2A priority Critical patent/CN110122095A/en
Publication of CN110122095A publication Critical patent/CN110122095A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • A01G2/38Holding; Ligating
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • A01G9/0293Seed or shoot receptacles
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

Practice seedling the present invention relates to the technical field of cotton tissue-cultured seedling culture more particularly to a kind of cotton tissue-cultured seedling and grafts dual-purpose nutrition set alms bowl and its application method.A kind of cotton tissue-cultured seedling practices seedling and the dual-purpose nutrition set alms bowl of grafting includes transparent interior inlay, the outer alms bowl of trophosome, humidity regulation cover and fixed plate, the transparent interior inlay is movable to be mounted in the outer alms bowl of the trophosome, the humidity regulation cover is detachably arranged in the outer alms bowl support of the trophosome along upper, the fixed plate is detachably arranged between the outer alms bowl of the trophosome and the humidity regulation cover, and the center of the fixed plate offers fixation hole.The present invention also provides a kind of cotton tissue-cultured seedling to practice the application method that seedling grafts dual-purpose nutrition set alms bowl.Fixed plate of the present invention makes cotton seedling not slant in water planting and grafting procedures;Apical growing point and young tender leaf are protected, avoids causing plant mechanical damage, adjusts humidity, improve the survival rate of cotton seedling.

Description

A kind of dual-purpose nutrition of cotton training tissue culture seedling grafting covers alms bowl and its application method
Technical field
The present invention relates to the technical field of cotton tissue-cultured seedling culture more particularly to a kind of grafting of cotton training tissue culture seedling are dual-purpose Nutrition covers alms bowl and its application method.
Background technique
Cotton tissue-cultured seedling (being commonly called as test tube seedling) means the cotton somatic obtained after tissue cultures or genetic transformation again Raw strain.Cotton tissue-cultured seedling moves into flowerpot from aseptic culture medium or crop field is the important link of tissue cultures, genetic transformation.In south Square tropical rainy climate, directly transplanting is not problem.But under the environmental condition of north drying, cotton regenerated plantlet of transplant is survived Difficult problem annoyings always the development of cotton biotechnology commercialization.Cotton tissue culture transplantation of seedlings be from it is sterile to have bacterium, from height Process of the warm super-humid conditions to normal condition transition.The key factor for influencing transplant survival is the development condition of tissue-cultured seedling, domestication Transition, the selection of transplanting medium, transplanting environment light, temperature, wet control and grasp of graft technology etc..Improve transplanting at Motility rate, it is necessary to grasp, control and make good use of these key factors, especially to do well to tame transition, and skillfully grasp grafting skill Art.
Environmental condition directly affects surviving for tissue-cultured seedling graft and transplantation, it is important to control the light for grafting latter week, temperature, It is wet.The necessary plastic covering film of the seedling just grafted or cover glass keep humidity, avoid direct sunlight, temperature is with 25~30 DEG C Preferably.Aperture is gradually put after one week, it is ascending, until removing plastic film or glass completely.
Cotton tissue-cultured seedling, toward when transplanting, need to pass through hardening, transplant seedlings, or by grafting seedling out of culture medium, Two processes are all more complex, it is desirable that stringent.Currently without dedicated for tissue culture hardening or the nutritive cube transplanted seedlings.In water planting and grafting When, Miao Rongyi slants, and apical growing point and young tender leaf are easy to touch plastic film or cover glass, causes machine to plant Tool damage;In addition not only troublesome in poeration with plastic film, and be not fixed easily;And humidity cannot be effectively adjusted with glass.
Summary of the invention
In view of the shortcomings of the prior art, grafting dual-purpose nutrition the present invention provides a kind of cotton training tissue culture seedling covers alms bowl.
A kind of dual-purpose nutrition of cotton training tissue culture seedling grafting covers alms bowl, humidity regulation outside alms bowl, including transparent interior inlay, trophosome Cover and fixed plate, the transparent interior inlay is movable to be mounted in the outer alms bowl of the trophosome, and the humidity regulation cover is dismountable The outer alms bowl support of the trophosome is mounted on along upper, the fixed plate is detachably arranged in the outer alms bowl of the trophosome and the humidity tune Between section cover, the fixed plate center offers fixation hole.The fixed plate prevents cotton seedling from slanting in water planting and grafting, reduces The mechanical damage of apical growing point and young tender leaf;Humidity regulation cover effectively adjusts humidity.The transparent interior inlay is movable The effect being mounted in the outer alms bowl of the trophosome is: in water planting hardening convenient for observation root condition.
Further, the humidity regulation cover includes housing, knob and gas adjustable plate, and the housing and the knob are living Dynamic connection, the knob lower part are fixedly connected with the gas regulation plate, the gas regulation plate equipped with on the housing The venthole of identical equidistant radiation.The housing and gas regulation plate rotation control the size of stomata when interlocking, into And control ventilatory capacity and humidity.
Further, the housing has arc track, and the knob is slidably mounted in the arc track, the knob Lower part is fixedly connected with the gas regulation plate.The track of the arc stirs knob along track with hand, drives gas regulation plate Rotation adjusts the humidity of air to make the dislocation in the full communication between the higher and lower levels hole of housing and gas adjustable plate.
Further, side wall a part of the housing is transparent side, the side wall rest part and the cover of the housing The top of shell is translucent frosting.Light direct beam is avoided to generate injury to seedling again convenient for observation.
Further, the knob is the step-like bolt positioned at the housing center, the step-like bolt top card At the top of the housing, the step-like bolt lower part is consolidated after passing through the housing and the gas regulation plate by nut It is fixed.
Further, through-hole is arranged at the bottom of the outer alms bowl of the trophosome.Be conducive to that extra moisture is discharged, prevent flooded seedling.
Further, the fixed plate is that two pieces of impermeable isotropic disks are constituted, and is equipped in the two sides interposition of the impermeable isotropic disk Semi-cylindrical canyon, the fixation hole are combined by the semi-cylindrical canyon on the inside of two pieces of impermeable isotropic disks.Fixed plate acts on In: give root system shading;Fixed tissue culture cotton seedling hypocotyl;It is also used for the fixation of graft stock.The outside semicircle is recessed It falls into and is conducive to taking and placing fixed plate.
Further, the outer alms bowl of the trophosome uses inverted round stage structure, and the bottom radius of the outer alms bowl of the trophosome is 4cm, Upper radius is 5cm.The humidity regulation cover uses frustum cone structure, the high 15cm of humidity regulation cover, the humidity regulation cover Bottom opening radius and the support edge of the outer alms bowl of the trophosome match, and the humidity regulation cover top radius is 3.5cm, Neng Goubao 4-5 leaf cotton seedling root system normal growth is demonstrate,proved, space waste is avoided.
The present invention also provides the application methods that a kind of cotton training tissue culture seedling grafts dual-purpose nutrition set alms bowl comprising following step It is rapid: Nutrition Soil being directly placed in alms bowl outside the trophosome, stock seed is seeded in the middle position of Nutrition Soil, waits stocks young After seedling is unearthed, stock seedling hypocotyl position is fixed with the fixed plate, grafting operation is carried out, has grafted, covered described wet Degree adjusts cover, and the transparent side side backlight of the housing is placed, and controls the gas regulation plate by the knob, adjusts wet Degree.
The present invention also provides the application methods that a kind of cotton training tissue culture seedling grafts dual-purpose nutrition set alms bowl comprising following step It is rapid: the transparent interior inlay being put into alms bowl outside the trophosome, the nutrient solution that water planting needs is added, by tissue-cultured seedling out of culture medium Take out be put into it is described it is transparent it is embedded in vivo, stock seedling hypocotyl position is fixed with the fixed plate, covers the humidity tune The transparent side side backlight of section cover, the housing is placed, and is controlled the gas regulation plate by the knob, is adjusted humidity.
Compared with prior art, the beneficial effects of the present invention are:
1, fixed plate of the present invention makes cotton seedling not slant in water planting and grafting procedures, improves surviving for cotton regenerated seedling Rate.
2, humidity regulation cover of the present invention can adjusting humidity easily and effectively, protect apical growing point and young tender leaf, effectively Adjusting humidity, improve the survival rate of cotton regenerated seedling.
3, the size of the outer alms bowl of the most suitable trophosome of the present invention advantageously ensures that 4-5 leaf cotton seedling root system normal growth, keeps away Exempt from space waste.
4, humidity regulation cover one side of the present invention is transparent, remaining 3 side and top are that frosted is translucent, convenient for observation Light direct beam is avoided to generate injury to seedling again.
5, the outer alms bowl of trophosome of the present invention and opaque fixed plate play the role of being protected from light during water planting, simulate soil Dark surrounds guarantees the normal growth of root.
6, the transparent embedded body of the present invention can observe root growth situation, can when the increment of water planting seedling root is enough And when transplant seedlings.
Figure of description
Fig. 1 is that cotton training tissue culture seedling grafts dual-purpose nutrition set alms bowl structural schematic diagram;
Fig. 2 is the outer alms bowl structural schematic diagram of trophosome;
Fig. 3 is the casing structure schematic diagram of carrying spigots;
Fig. 4 is the structural schematic diagram of gas regulation plate;
Fig. 5 is the structural schematic diagram of fixed plate;
Fig. 6 is that cotton training tissue culture seedling grafts dual-purpose nutrition set alms bowl structural schematic diagram;
Fig. 7 is the outer alms bowl structural schematic diagram of trophosome;
Fig. 8 is casing structure schematic diagram;
Fig. 9 is the casing structure schematic diagram of carrying spigots;
Figure 10 is the structural schematic diagram of fixed plate;
Figure 11 is the structural schematic diagram of gas regulation plate;
Figure 12 is the structural schematic diagram at the top of housing;
Figure 13 is the structural schematic diagram of the connection housing of step-like bolt.
Drawing reference numeral: 1, the outer alms bowl of trophosome;12, hold in the palm edge;13, through-hole;, 2, transparent interior inlay;3, fixed plate;31, semicircle Shape recess;32, fixation hole;4, housing;41, socket;42, arc track;43 ventholes;5, knob;51, step-like bolt;52, Nut;6, gas regulation plate;61, venthole;7, humidity regulation cover.
Specific embodiment
The present invention is further explained in the light of specific embodiments, so that those skilled in the art can be better Understand the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1
A kind of cotton training tissue culture seedling using cylindrical structure as shown in Figure 1 grafts dual-purpose nutrition and covers alms bowl, can group Two kinds of structures are dressed up, the transplanting of grafting and the transplanting of tissue-cultured seedling are respectively used to.
The first structure does not include transparent interior inlay 2, the transplanting for grafting.As shown in Figs. 1-5, using cylindrical body knot A kind of cotton training tissue culture seedling of structure grafts alms bowl 1, humidity regulation cover 7 and fixed plate 3 outside dual-purpose nutrition set alms bowl, including trophosome, It is equipped with support at the top of the outer alms bowl 1 of trophosome along 12, holds in the palm the support platform for forming a L-type at the top of alms bowl 1 outside 12 and trophosome.Fixed plate 3 are mounted on support along 12, i.e. the support platform that is fastened on L-type of fixed plate 3.3 center of fixed plate is offered for fixing stock children The fixation hole 32 of seedling hypocotyl.Fixation hole 32 prevents cotton seedling from slanting in grafting, reduces cotton seedling apical growing point and children The mechanical damage of tender leaf.Humidity regulation cover 7 is buckled in support along 12, that is, is fastened in the support platform of L-type.Humidity regulation cover 7 It is effective to adjust humidity.
Second of structure includes transparent interior inlay 2, the transplanting for tissue-cultured seedling.As shown in Figs. 1-5, using cylindrical structure A kind of cotton training tissue culture seedling graft alms bowl 1, transparent interior inlay 2, humidity regulation cover 7 outside dual-purpose nutrition set alms bowl, including trophosome With fixed plate 3, transparent interior inlay 2 is mounted in the outer alms bowl 1 of trophosome, and transparent interior inlay 2 is cylindrical.Outer 1 top of alms bowl of trophosome Equipped with support along 12, the support platform along 12 and one L-type of formation at the top of alms bowl 1 outside trophosome is held in the palm.Fixed plate 3 is mounted on support along 12 On, i.e. the support platform that is fastened on L-type of fixed plate 3.3 center of fixed plate opens up fixation hole 32, for fixing embryo under tissue culture seedling Axis.Fixation hole 32 prevents cotton seedling from slanting in grafting, reduces the mechanical damage of cotton seedling apical growing point and young tender leaf. Humidity regulation cover 7 is buckled in support along 12 tops, is fastened in the support platform of L-type.Humidity regulation cover 7 effectively adjusts humidity.Gu Fixed board 3 is mounted between the outer alms bowl 1 of trophosome and humidity regulation cover.
As shown in Figure 3 and Figure 4, humidity regulation cover includes housing 4, knob 5 and gas adjustable plate 6, and housing 4 and knob 5 are living Dynamic connection, knob 5 are fixedly connected with gas regulation plate 6, are bonded at the top of gas regulation plate 6 and housing 4, set on gas regulation plate 6 There is the venthole 61 with equidistant radiation identical on housing.Control stomata is big when housing 4 and the rotation of gas adjustable plate 6 interlock It is small, and then control ventilatory capacity and humidity.
About 4 relative rotation of adjustable plate 6 and housing, two kinds of structures can be used.
The first structure is as shown in figure 13, and knob 5 is the step-like bolt 51 positioned at 4 center of housing, step-like bolt 51 Top is stuck in the top of housing, and step-like 51 lower part of bolt passes through 4 top of housing, then is fixedly connected with gas regulation by nut 52 Plate 6.Gas regulation plate 6 is driven to rotate by rotating step-like bolt 51, so that housing 4 and the upper and lower of gas adjustable plate 6 be made to lead to The dislocation of stomata adjusts the humidity of air.
Second of structure is as shown in Figure 3 and Figure 4, and housing 4 has arc track 42, and 5 lower part of knob passes through arc track 42, gas A mounting hole is offered on 6 edge of body adjustable plate, is cooperated with knob 5, and 5 lower part of knob and the fixed company of gas adjustable plate 6 are made It connects.With hand, arcuately track 42 stirs knob 5, will drive gas regulation plate 6 and rotates along arc track 42.To make housing 4 With the dislocation in the full communication between the higher and lower levels hole of gas adjustable plate 6, the humidity of air is adjusted.
Side wall a part of housing 4 is transparent side, and the side wall rest part of housing and the top of housing are translucent frosted Face.
As shown in figure 3, the one or both sides of housing 4 are provided with socket 41.Socket is conducive to install gas regulation plate 6.
As shown in Fig. 2, through-hole 13 is arranged at the bottom of the outer alms bowl 1 of trophosome.Through-hole 13 is conducive to that extra moisture is discharged, and prevents flood Seedling.
As shown in figure 5, fixed plate 3 is that two opaque semicircular plates are constituted, in the inside interposition of semicircular plate It is equipped with semi-cylindrical canyon, the semi-cylindrical canyon of two pieces of semicircular plates is combined into a complete fixation hole 32, which is used for Insert graft stock.Fixed plate 3 is used for the fixation of graft stock, and the semi-cylindrical canyon 31 opened up on the outside of semicircular plate is conducive to take Put fixed plate.
Embodiment 2
Dual-purpose nutrition is grafted using a kind of cotton training tissue culture seedling of rectangular parallelepiped structure as shown in Fig. 6-Figure 12 and covers alms bowl, it can To be assembled into two kinds of structures, it is respectively used to the transplanting of grafting and the transplanting of tissue-cultured seedling.
The first structure does not include transparent interior inlay, the transplanting for grafting.As shown in fig. 6, using rectangular parallelepiped structure A kind of cotton training tissue culture seedling graft alms bowl 1, humidity regulation cover 7 and fixed plate 3 outside dual-purpose nutrition set alms bowl, including trophosome, battalion It supports and is equipped with support at the top of external alms bowl 1 along 12, hold in the palm the support platform along 12 and one L-type of formation at the top of alms bowl 1 outside trophosome.Fixed plate 3 Support is mounted on along 12, i.e. the support platform that is fastened on L-type of fixed plate 3.3 center of fixed plate is offered for fixing stock seedling The fixation hole 32 of hypocotyl.Fixation hole 32 prevents cotton seedling from slanting in grafting, reduces cotton seedling apical growing point and children is tender The mechanical damage of leaf.Humidity regulation cover 7 is buckled in support along 12 tops, that is, is fastened in the support platform of L-type.Humidity regulation cover 7 It is effective to adjust humidity.
Second of structure includes transparent interior inlay 2, the transplanting for tissue-cultured seedling.Including alms bowl 1, transparent interior inlay outside trophosome 2, humidity regulation cover 7 and fixed plate 3, transparent interior inlay 2 are mounted in the outer alms bowl 1 of trophosome, and transparent interior inlay 2 is cuboid.Battalion It supports and is equipped with support at the top of external alms bowl 1 along 12, hold in the palm the support platform along 12 and one L-type of formation at the top of alms bowl 1 outside trophosome.Fixed plate 3 Support is mounted on along 12, i.e. the support platform that is fastened on L-type of fixed plate 3.3 center of fixed plate opens up fixation hole 32, for fixing Tissue culture seedling hypocotyl.Fixation hole 32 prevents cotton seedling from slanting in grafting, reduces cotton seedling apical growing point and young tender leaf The mechanical damage of son.Humidity regulation cover 7 is buckled in support along 12 tops, is fastened in the support platform of L-type.Humidity regulation cover 7 is effective Adjusting humidity.Fixed plate 3 is mounted between the outer alms bowl 1 of trophosome and humidity regulation cover.
As shown in fig. 6, humidity regulation cover includes housing 4, knob 5 and gas adjustable plate 6, housing 4 and 5 activity of knob are even Connect, knob 5 is fixedly connected with gas regulation plate 6, be bonded at the top of gas regulation plate 6 and housing 4, gas regulation plate 6 be equipped with The venthole 61 of identical equidistant radiation on housing 4.Housing 4 and the rotation of gas adjustable plate 6 control the size of stomata when interlocking, And then control ventilatory capacity and humidity.
Side wall a part of housing 4 is transparent side, and the side wall rest part of housing and the top of housing are translucent frosted Face.
As shown in Fig. 8-Figure 12, housing 4 has arc track 42, and the length and width of gas regulation plate 6 are respectively less than the length and width of housing 4, 5 lower part of knob passes through arc track 42, offers a mounting hole on 6 edge of gas regulation plate, is connected through a screw thread with knob 5 , it is fixedly connected with 5 lower part of knob with gas adjustable plate 6.With hand, arcuately track 42 stirs knob 5, will drive gas regulation Plate 6 is rotated along arc track 42.To make the dislocation in the full communication between the higher and lower levels hole of housing 4 and gas adjustable plate 6, the wet of air is adjusted Degree.
As shown in figure 9,4 one or both sides of housing are provided with socket 41, socket is conducive to install gas regulation plate 6.
As shown in fig. 7, through-hole 13 is arranged at outer 1 bottom of alms bowl of trophosome.Through-hole 13 is conducive to that extra moisture is discharged, and prevents flood Seedling.
As shown in Figure 10, fixed plate 3 is that two opaque rectangular plates are constituted, in the two sides interposition of rectangular slab It is equipped with semi-cylindrical canyon 31.Semi-cylindrical canyon on two pieces of rectangular slabs is combined into a complete fixation hole 32, the fixation hole 32 For fixing the hypocotyl of tissue-cultured seedling.The semi-cylindrical canyon 31 opened up on the outside of rectangular slab is conducive to taking and placing fixed plate.
Embodiment 3
The outer alms bowl 1 of trophosome uses inverted round stage structure, and the bottom radius of the outer alms bowl 1 of trophosome is 4cm, upper radius 5cm, wet Degree adjusts 7 high 15cm of cover, and humidity regulation cover 7 uses frustum cone structure, and 7 top radius of humidity regulation cover is 3.5cm, can guarantee 4- 5 leaf cotton seedling root system normal growths, avoid space waste.Rest part is identical as Examples 1 and 2.
Embodiment 5
A kind of cotton training tissue culture seedling grafts the application method that dual-purpose nutrition set alms bowl is used for grafting comprising following step It is rapid: directly Nutrition Soil being placed in outside trophosome in alms bowl, stock seed is seeded in Nutrition Soil middle position, stocks seedling is waited to go out After soil, stock seedling hypocotyl position is fixed by fixed plate, grafting operation is carried out, has grafted, covered humidity regulation cover, pass through Knob controls gas regulation plate, adjusts humidity.This method can be used for stock plantation, fixed and grafting management.Housing it is saturating Bright side backlight is placed, and light direct beam is avoided to generate injury to seedling.
Embodiment 6
A kind of cotton training tissue culture seedling grafts the application method that dual-purpose nutrition set alms bowl is used for tissue-cultured seedling comprising following step It is rapid: transparent interior inlay being put into outside trophosome in alms bowl, the nutrient solution that water planting needs is added, tissue-cultured seedling is taken out out of culture medium and is put Enter it is transparent it is embedded in vivo, tissue-cultured seedling hypocotyl position is fixed with fixed plate, humidity regulation cover is covered, gas is controlled by knob Adjustable plate adjusts humidity.This method can be used for the management of tissue-cultured seedling water planting hardening.The transparent side backlight of housing is placed, and is avoided Light direct beam generates injury to seedling.
Above embodiments are only to absolutely prove preferred embodiment that is of the invention and being lifted, and protection scope of the present invention is not It is limited to this.Those skilled in the art's made equivalent substitute or transformation on the basis of the present invention, in guarantor of the invention Within the scope of shield.Protection scope of the present invention is subject to claims.

Claims (9)

1. a kind of cotton training tissue culture seedling, which grafts dual-purpose nutrition, covers alms bowl, it is characterised in that: outside including transparent interior inlay, trophosome Alms bowl, humidity regulation cover and fixed plate, the transparent interior inlay is movable to be mounted in the outer alms bowl of the trophosome, the humidity regulation Cover is detachably arranged in the support of the outer alms bowl of the trophosome along upper, and the fixed plate is detachably arranged in the outer alms bowl of the trophosome Between the humidity regulation cover, the center of the fixed plate offers fixation hole.
2. grafting dual-purpose nutrition according to cotton training tissue culture seedling described in claim 1 covers alms bowl, it is characterised in that: the humidity regulation Cover includes housing, knob and gas adjustable plate, and the housing is flexibly connected with the knob, the lower part of the knob and the gas Body adjustable plate is fixedly connected, and the gas regulation plate is equipped with the venthole with equidistant radiation identical on the housing.
3. grafting dual-purpose nutrition according to cotton training tissue culture seedling described in claim 2 covers alms bowl, it is characterised in that: the housing has arc Shape track, the knob are slidably mounted in the arc track, the lower part of the knob and the fixed company of the gas regulation plate It connects.
4. grafting dual-purpose nutrition according to cotton training tissue culture seedling described in claim 2 covers alms bowl, it is characterised in that: the side of the housing Wall a part is transparent side, and the side wall rest part of the housing and the top of the housing are translucent frosting.
5. grafting dual-purpose nutrition according to cotton training tissue culture seedling described in claim 2 covers alms bowl, it is characterised in that: the knob is position Step-like bolt in the housing center, the step-like bolt top are stuck in the top of the housing, the step-like spiral shell Bolt lower part is fixed after passing through the housing and the gas regulation plate by nut.
6. grafting dual-purpose nutrition according to any cotton training tissue culture seedling of claim 1-5 covers alms bowl, it is characterised in that: the battalion Through-hole is arranged at the bottom for supporting external alms bowl.
7. grafting dual-purpose nutrition according to any cotton training tissue culture seedling of claim 1-5 covers alms bowl, it is characterised in that: described solid Fixed board is that two pieces of impermeable isotropic disks are constituted, and is equipped with semi-cylindrical canyon in the two sides interposition of the impermeable isotropic disk, the fixation hole is It is combined by the semi-cylindrical canyon on the inside of two pieces of impermeable isotropic disks.
8. grafting the application method of dual-purpose nutrition set alms bowl by any cotton training tissue culture seedling of claim 1-4 comprising with Lower step: being directly placed in alms bowl outside the trophosome for Nutrition Soil, and stock seed is seeded in the middle position of Nutrition Soil, waits anvils After wood comes up, stock seedling hypocotyl position is fixed with the fixed plate, grafting operation is carried out, has grafted, covered institute Humidity regulation cover is stated, the transparent side side backlight of the housing is placed, and is controlled the gas regulation plate by the knob, is adjusted Humidity.
9. grafting the application method of dual-purpose nutrition set alms bowl by any cotton training tissue culture seedling of claim 1-4 comprising with Lower step: being put into alms bowl outside the trophosome for the transparent interior inlay, and the nutrient solution that water planting needs is added, by tissue-cultured seedling from culture Taken out in base be put into it is described it is transparent it is embedded in vivo, stock seedling hypocotyl position is fixed with the fixed plate, is covered described wet Degree adjusts cover, and the transparent side side backlight of the housing is placed, and controls the gas regulation plate by the knob, adjusts wet Degree.
CN201910542112.2A 2019-06-21 2019-06-21 A kind of dual-purpose nutrition of cotton training tissue culture seedling grafting covers alms bowl and its application method Pending CN110122095A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111557233A (en) * 2020-05-26 2020-08-21 祁维销 Multipurpose vegetable soilless culture facility

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KR200288931Y1 (en) * 2002-06-26 2002-09-10 김영학 Portable apparatus for cultivating mini cactus
KR200377697Y1 (en) * 2004-12-03 2005-03-11 (주)스타씨엔에이 A plant cultivation vessel
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CN204742124U (en) * 2015-05-26 2015-11-11 昆明理工大学 Plant tissue culture bottle lid of adjustable throughput
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CN111557233B (en) * 2020-05-26 2021-11-26 特隆凯丰(福建)科技集团有限公司 Multipurpose vegetable soilless culture facility

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