CN206244815U - A kind of novel cell culture bottle - Google Patents
A kind of novel cell culture bottle Download PDFInfo
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- CN206244815U CN206244815U CN201621360610.3U CN201621360610U CN206244815U CN 206244815 U CN206244815 U CN 206244815U CN 201621360610 U CN201621360610 U CN 201621360610U CN 206244815 U CN206244815 U CN 206244815U
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- plug
- glass body
- bottle
- bottleneck
- cell culture
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model relates to a kind of novel cell culture bottle, including glass body, bottleneck.Glass body upper end is provided with bottleneck, the upper end of bottleneck is connected with plug, plug through hole is provided with the middle of plug, respiratory siphon is provided with plug through hole, ventilated membrane is housed in the middle of respiratory siphon, in respiratory siphon insertion plug through hole, uviol lamp is provided with the upper end of vial and the leftward position of bottleneck, LED is provided with the upper end of vial and the right positions of bottleneck；The left surface on glass body top is provided with thermometer, and the right flank on glass body top is provided with exhaust tube, water inlet pipe, and the middle and lower part side of glass body is provided with rubber sleave, and the lower end of glass body is provided with base, mica heating plate is provided with base.The utility model advantage is：Light in temperature in blake bottle, humidity, ph values, bottle can be adjusted, can be cell oxygen supply, improve cell quality.It is low-oxygen environment that can make in bottle, can also adjust the concentration of nutrient solution, is easy to the culture and experiment of biological cell.
Description
Technical field
The utility model is related to biological cell culture utensil, and in particular to a kind of novel cell culture bottle.
Background technology
The current a large amount of glass Tissue Culture Flasks for using of production of vaccine and research and development, but all breathed without design and installation
Pipe, causing cell growth to breed metabolic process can not take a breath, and making the waste gas of cell hypoxia gas and carbon dioxide, generation cannot hand over
Change, be suppressed the metabolism of cell, cell quality declines；Contain substantial amounts of bacterium in vial, cause cell death, data
It is inaccurate；The conditions such as temperature and humidity, the luminous energy of the cell peripheral in vial, for cell culture and experiment all right and wrong
It is often important.
Utility model content
The purpose of this utility model is to overcome defect of the prior art, designs a kind of novel cell culture bottle, is solved
In existing Tissue Culture Flask, the problem that can not be taken a breath in cell cultivation process, while solving bacterium in bottle excessively influences reality
The problem of cell culture is tested or influences, while glass body internal temperature, the number of oxygen can be detected and according to experiment
Condition is to supplementing a small amount of water in bottle.
To achieve the above object, the technical scheme that the utility model is used is a kind of novel cell culture bottle, including glass
Glass bottle, bottleneck.Glass body upper end is provided with bottleneck, and the upper end of bottleneck is connected with plug, and plug through hole, glue are provided with the middle of plug
It is provided with plug through hole and ventilated membrane is housed in the middle of respiratory siphon, respiratory siphon, in respiratory siphon insertion plug through hole.By respiratory siphon and ventilative
Film can be cell oxygen supply；Carbon dioxide can be supplied for cell nutrient solution, be easy to adjust ph values in nutrient solution.Vial it is upper
Uviol lamp is provided with end and the leftward position of bottleneck, when vial is hollow, uviol lamp can be opened, entered with ultraviolet light
The effect of row sterilizing.LED is provided with the upper end of vial and the right positions of bottleneck, can provide what is become clear for experiment
Environment, and can be to carrying out light filling in nutrient solution；The left surface on glass body top is provided with thermometer, can detect blake bottle
In temperature, and then find one be adapted to culture environment.The right flank on glass body top is provided with exhaust tube, water inlet pipe, takes out
Tracheae can transfer the air in blake bottle, such that it is able to reach the state of low-oxygen environment or anaerobic in bottle, cultured cells or
Person does some data that experiment is obtained.Water inlet pipe can be to a small amount of water be supplemented, so as to nutrient solution can also be adjusted in blake bottle
In ph values.The middle and lower part side of glass body is provided with rubber sleave, and rubber sleeve with protective glass bottle, and can take up
When may also operate as anti-skidding effect；The lower end of glass body is provided with base, and submounts are provided with groove, cloud is put into groove
Female heating plate.Mica heating plate can provide an environment for relative matters for Tissue Culture Flask, while can also be in empty bottle shape
Under state, the effect of heating disinfection is carried out.
Preferably, the cross section of respiratory siphon is square.
Preferably, uviol lamp is provided with circuit, and circuit upper end is connected with plug.Under empty bottle state, uviol lamp is opened, it is purple
UV light carries out sterilizing, reduces some bacteria effect cell culture.
Preferably, LED is provided with circuit, and circuit upper end is connected with plug.LED can mainly carry out light filling, to thin
One relatively bright environment of born of the same parents.
Preferably, the upper end of plug is provided with anti-slip veins, can play anti-skidding effect.
Preferably, rubber sleave side is provided with anti-slip veins, can play anti-skidding effect.
Preferably, right-hand member is provided with through hole on base, and mica heating plate is provided with circuit, and circuit is located in base plate through holes, line
Road right-hand member is connected with plug.Mica heating plate mainly can be to provide some heats in Tissue Culture Flask.
Preferably, it is provided with screen pack in water inlet pipe.Screen pack can filter out some impurity in some water, can carry out
Humidity in regulation blake bottle.
The course of work of the present utility model is：When what is all no in blake bottle, uviol lamp is opened in blake bottle
Bacterium carries out sterilizing, and exhaust tube can all evacuate the air in bottle, reduces aerobic bacteria and virus.Nutrient solution with
And the cell of culture is needed by being cultivated in bottleneck upper port importing blake bottle.Mended in bottle by respiratory siphon and ventilated membrane
Fill oxygen and carbon dioxide, the environment in regulation culture, while can also adjust the ph values in nutrient solution.Detected by thermometer and trained
Temperature in bottle is supported, can be by mica heating plate to being heated in blake bottle.Open LED can be to be mended in blake bottle
Light, there is provided a bright and clear environment, ph values can be adjusted by water inlet pipe, adjust the concentration of nutrient solution.
Advantage of the present utility model and beneficial effect are：Can adjust in temperature in blake bottle, humidity, ph values, bottle
Light.There are spiracle and ventilated membrane, can be cell oxygen supply；Carbon dioxide can be supplied for cell nutrient solution, be easy to nutrient solution to adjust
ph；Can discharge cell metabolism waste gas；Ventilated membrane can stop that external microbe enters simultaneously；Ensure that cell normal growth is numerous
Grow, improve cell quality.It is low-oxygen environment that can make in bottle, can also adjust the concentration of nutrient solution, is easy to biological cell
Culture and experiment.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model novel cell culture bottle.
Fig. 2 is the utility model novel cell culture bottle plug top view.
In Fig. 1：1st, plug；2nd, respiratory siphon；3rd, ventilated membrane；4th, circuit；5th, plug；6th, LED；7th, exhaust tube；8th, filter
Net；9th, water inlet pipe；10th, rubber sleave；11st, base；12nd, mica heating plate；13rd, thermometer；14th, glass body；15th, bottleneck；
16th, groove.
In Fig. 2：21st, plug through hole.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiment of the present utility model is further described.Hereinafter implement
Example is only used for clearly illustrating the technical solution of the utility model, and protection model of the present utility model can not be limited with this
Enclose.
Embodiment 1
As shown in Figure 1：The present embodiment is a kind of novel cell culture bottle, including glass body 14, bottleneck 15.Glass body
14 upper ends are provided with bottleneck 15, and the upper end of bottleneck 15 is connected with plug 1, and the through hole of plug 1 is provided with the middle of plug 1, are set in the through hole of plug 1
There is respiratory siphon 2, ventilated membrane 3 is housed, in the insertion through hole of plug 1 of respiratory siphon 2 in the middle of respiratory siphon 2.By respiratory siphon 2 and ventilated membrane 3
It can be cell oxygen supply；Carbon dioxide can be supplied for cell nutrient solution, be easy to adjust ph values in nutrient solution.The upper end of vial
Uviol lamp is provided with the leftward position of bottleneck 15, when vial is hollow, uviol lamp can be opened, entered with ultraviolet light
The effect of row sterilizing.LED 6 is provided with the upper end of vial and the right positions of bottleneck 15, can provide bright for experiment
Bright environment, and can be to carrying out light filling in nutrient solution；The left surface on the top of glass body 14 is provided with thermometer 13, Ke Yijian
The temperature surveyed in blake bottle, and then find an environment for being adapted to culture.The right flank on the top of glass body 14 is provided with exhaust tube
7th, water inlet pipe 9, exhaust tube 7 can transfer the air in blake bottle, such that it is able to reach the shape of low-oxygen environment or anaerobic in bottle
State, cultured cells or does some data for obtaining of experiment.Water inlet pipe 9 can to supplementing a small amount of water in blake bottle so that
The ph values in nutrient solution can be adjusted.The middle and lower part side of glass body 14 is provided with rubber sleave 10, and rubber sleeve can protect glass
Glass bottle 14, and anti-skidding effect is may also operate as when taking up；The lower end of glass body 14 is provided with base 11, base
11 are provided with groove 16 above, and mica heating plate 12 is put into groove 16.Mica heating plate 12 can provide one for Tissue Culture Flask
The environment of individual relative matters, while the effect of heating disinfection under empty bottle state, can also be carried out.
The cross section of respiratory siphon 2 is square.
Uviol lamp is provided with circuit 4, and the upper end of circuit 4 is connected with plug 5.Under empty bottle state, uviol lamp, ultraviolet light are opened
Line carries out sterilizing, reduces some bacteria effect cell culture.
LED 6 is provided with circuit 4, and the upper end of circuit 4 is connected with plug 5.LED 6 can mainly carry out light filling, to cell
One relatively bright environment.
The upper end of plug 1 is provided with anti-slip veins, can play anti-skidding effect.
The side of rubber sleave 10 is provided with anti-slip veins, can play anti-skidding effect.
Right-hand member is provided with through hole on base 11, and mica heating plate 12 is provided with circuit 4, and circuit 4 is located in the through hole of base 11, line
The right-hand member of road 4 is connected with plug 5.Mica heating plate 12 mainly can be to provide some heats in Tissue Culture Flask.
Screen pack 8 is provided with water inlet pipe 9.Screen pack 8 can filter out some impurity in some water, can be adjusted
Humidity in blake bottle.
The course of work of this embodiment is：When what is all no in blake bottle, uviol lamp is opened in blake bottle
Bacterium carries out sterilizing, and exhaust tube 7 can all evacuate the air in bottle, reduces aerobic bacteria and virus.Nutrient solution
And the cell of culture is needed by being cultivated in the upper port of bottleneck 15 importing blake bottle.Given by respiratory siphon 2 and ventilated membrane 3
Supplemental oxygen and carbon dioxide in bottle, the environment in regulation culture, while can also adjust the ph values in nutrient solution.By thermometer
Temperature in 13 detection blake bottles, can be by mica heating plate 12 to being heated in blake bottle.It can be training to open LED 6
Light filling is carried out in foster bottle, there is provided a bright and clear environment, ph values can be adjusted by water inlet pipe 9, adjust the dense of nutrient solution
Degree.
Advantage of this embodiment is that：Light in temperature in blake bottle, humidity, ph values, bottle can be adjusted.There is spiracle
And ventilated membrane, can be cell oxygen supply；Carbon dioxide can be supplied for cell nutrient solution, be easy to nutrient solution to adjust ph；Cell generation can be made
Thank to waste gas discharge；Ventilated membrane can stop that external microbe enters simultaneously；Ensure that cell normal growth is bred, improve cytoplasm
Amount.It is low-oxygen environment that can make in bottle, can also adjust the concentration of nutrient solution, is easy to the culture and experiment of biological cell.
The above is only preferred embodiment of the present utility model, it is noted that for the common skill of the art
For art personnel, on the premise of the utility model know-why is not departed from, some improvements and modifications can also be made, these change
Enter and retouch also to should be regarded as protection domain of the present utility model.
Claims (8)
1. a kind of novel cell culture bottle, it is characterised in that including glass body, bottleneck；Glass body upper end is provided with bottleneck, bottle
The upper end of neck is connected with plug, and plug through hole is provided with the middle of plug, is provided with plug through hole in the middle of respiratory siphon, respiratory siphon equipped with saturating
Air film, in respiratory siphon insertion plug through hole；
Uviol lamp is provided with the upper end of vial and the leftward position of bottleneck, is set at the upper end of vial and the right positions of bottleneck
There is LED；The left surface on glass body top is provided with thermometer, and the right flank on glass body top is provided with exhaust tube, water inlet pipe,
The middle and lower part side of glass body is provided with rubber sleave；The lower end of glass body is provided with base；Submounts are provided with groove, groove
In be put into mica heating plate.
2. novel cell culture bottle as claimed in claim 1, it is characterised in that the cross section of respiratory siphon is square.
3. novel cell culture bottle as claimed in claim 1, it is characterised in that uviol lamp is provided with circuit, circuit upper end with
Plug is connected.
4. novel cell culture bottle as claimed in claim 1, it is characterised in that LED is provided with circuit, circuit upper end with insert
Head connection.
5. novel cell culture bottle as claimed in claim 1, it is characterised in that the upper end of plug is provided with anti-slip veins.
6. novel cell culture bottle as claimed in claim 1, it is characterised in that rubber sleave side is provided with anti-slip veins.
7. novel cell culture bottle as claimed in claim 1, it is characterised in that right-hand member is provided with through hole, mica heating on base
Plate is provided with circuit, and circuit is located in base plate through holes, and circuit right-hand member is connected with plug.
8. novel cell culture bottle as claimed in claim 1, it is characterised in that screen pack is provided with water inlet pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621360610.3U CN206244815U (en) | 2016-12-13 | 2016-12-13 | A kind of novel cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621360610.3U CN206244815U (en) | 2016-12-13 | 2016-12-13 | A kind of novel cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206244815U true CN206244815U (en) | 2017-06-13 |
Family
ID=59006475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621360610.3U Active CN206244815U (en) | 2016-12-13 | 2016-12-13 | A kind of novel cell culture bottle |
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| Country | Link |
|---|---|
| CN (1) | CN206244815U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498776A (en) * | 2021-06-17 | 2021-10-15 | 深圳逗点生物技术有限公司 | Biological transport system, biological transport method, and blood cell preservation system |
-
2016
- 2016-12-13 CN CN201621360610.3U patent/CN206244815U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498776A (en) * | 2021-06-17 | 2021-10-15 | 深圳逗点生物技术有限公司 | Biological transport system, biological transport method, and blood cell preservation system |
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