CN103493732B - A kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts - Google Patents
A kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts Download PDFInfo
- Publication number
- CN103493732B CN103493732B CN201310430971.5A CN201310430971A CN103493732B CN 103493732 B CN103493732 B CN 103493732B CN 201310430971 A CN201310430971 A CN 201310430971A CN 103493732 B CN103493732 B CN 103493732B
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- China
- Prior art keywords
- bottle
- blake bottle
- spore
- plug
- wireway
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- 238000004114 suspension culture Methods 0.000 title claims abstract description 7
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 239000007789 gas Substances 0.000 abstract description 17
- 241000195493 Cryptophyta Species 0.000 abstract description 15
- 230000001954 sterilising effect Effects 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 6
- 238000011534 incubation Methods 0.000 abstract description 5
- 238000002834 transmittance Methods 0.000 abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 3
- 230000003321 amplification Effects 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 5
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts, its parts comprise blake bottle, plug, air supply pipe, wireway, tampon, gas distributor, heat exchanger, support bar, spore collector. This device can ensure that dissolved oxygen abundance, light transmittance are high, are suitable for algal grown; Heat exchanger is placed in bottle and directly controls temperature, neither can affect the efficiency of light energy utilization and also ensure temperature controlled accurately quick simultaneously; The operation that can collect algae spore in incubation, greatly simplify traditional incubation extraction spore by set up spore collector in bottle, is convenient to the further amplification that algae kind is cultivated; In addition, dismounting of the present invention is easy, is convenient to clean, and sterilization method is simple, is particularly useful for the pure culture of macro.
Description
Technical field
The present invention relates to algae culture technical field, relate in particular to a kind of controllable temperature with Spore adhesion parts largeType algae suspension culture apparatus.
Background technology
Macro all has a large amount of distributions in poisons in freshwater, Marine water, and its purposes is in extensive range, according to productKind difference to can be used as food directly edible or for the extraction of functional component. No matter which kind of application form,All must there is efficient culture technique as support.
Existing macro culture technique can be divided into open cultivation and closed cultivation two classes, wherein openFormula is cultivated and conventionally in environment, is carried out in the open, and frond and culture medium directly contact with external environment. This cultivation sideMethod is relatively extensive, and environmental condition control accuracy is lower, is mainly used in product purity is required to lower purposes.And typically referring to the culture medium inoculated algae kind of utilizing in closed environment after sterilizing, closed cultivation cultivates, canEffectively to avoid outside contamination, improve and cultivate purity and precision.
The closed cultivation of macro, requires to have culture process efficient, that optimize on the one hand, and the opposing party needsComplete function, culture apparatus reasonable in design are supported. Comprehensive, reasonable in design the referring to of called function cultivated dressThe design selection of putting can fully meet the demand of culture process, conventionally should meet the following conditions: have goodLight transmittance; Can effectively control temperature conditions; Can provide sufficient, uniform aseptic gas for algal grown;Other functional characteristics.
The closed culture apparatus of algae of prior art, has conventionally all considered and has met good light transmittance and abundanceThe condition that gas is supplied with, but its temperature control generally regulates by ambient temperature or outside culture apparatusPortion arranges heat exchange chuck and realizes, but these two kinds of methods all exist obvious defect: the former temperature regulates slowly,Energy consumption is large; The latter affects light transmittance.
In addition, in the incubation of algae, especially conventionally need to obtain its filial generation for putting in pure culture processLarge cultivation or preservation, but the closed culture apparatus of the algae of prior art does not have corresponding design conventionally,Therefore the operation that causes obtaining macro spore in incubation is more difficult.
Summary of the invention
The present invention is directed to the technological deficiency of prior art, provide one to there is more excellent temperature control capability, Neng GouThe efficient device of cultivating for the closed suspension inflation of macro of collecting algae spore when cultivation.
For realizing above technical purpose, the present invention takes following technical scheme.
With a controllable temperature macro suspension culture apparatus for Spore adhesion parts, comprise blake bottle, plug,Air supply pipe, wireway, tampon, gas distributor, characterized by further comprising heat exchanger, support bar, sporeSub-collector, wherein, the bottleneck of plug jam-pack blake bottle; Air supply pipe inserts and trains in the middle of plug and verticallySupport bottle inner; The rectangular shape of wireway, one of them limit is in the middle of plug and in vertical insertion blake bottlePortion, another limit and summit, right angle are positioned at blake bottle outside; Tampon be placed in another limit of above-mentioned wireway inThe position of portion, close port; Gas distributor is positioned at blake bottle inside and is fixedly connected with air supply pipe lower end; HeatInterchanger also vertically inserts blake bottle inside in the middle of plug; Support bar is inserted in the middle of plug and verticallyEnter blake bottle inside; Spore collector is positioned at blake bottle inside and is fixedly connected with support bar lower end.
Wherein, described heat exchanger lower end is blake bottle bottleneck to bottle end distance to the distance at the bottom of blake bottle bottle1/6; Described gas distributor lower end is that blake bottle bottleneck is to 1/3 of bottle end distance to the distance at the bottom of blake bottle bottle;Described support bar lower end is that blake bottle bottleneck is to 2/3 of bottle end distance to the distance at the bottom of blake bottle bottle; Described air guidePipe lower end is higher than described support bar lower end, and described wireway lower end and described support bar lower end are in the vertical direction simultaneouslyOn distance be 3-5cm.
In addition, in this culture apparatus, tampon is is left unlocked or unlatched shape, does not block the gas flow in described wireway,Its effect is to alleviate to a certain extent environment in bottle interior material loss and the outer contaminants bottle of bottle; Described confessionBetween tracheae and described gas distributor, there is gas interchange channel, can be by from the external world in device runningThe gas passing into from air supply pipe input, after gas distributor is broken up all with distribute and bottle in liquid environment; InstituteState heat exchanger and can control blake bottle interior environment temperature, itself can either heat supply also can cooling; DescribedHot spore collector can be collected the spore in its surrounding environment; The material of described blake bottle is glass.
This device in use, first carries out sterilization treatment by above-mentioned all parts, then in gnotobasis by instituteHave parts according to said structure feature connect complete, then by algae kind bondage to be cultivated in gas distributor periphery,The culture medium after sterilizing is joined in bottle, making culture medium liquid level is distance at the bottom of bottleneck arrives bottle to the distance at the bottle end again2/3; Then, utilize heat exchanger that liquid environment temperature in bottle is adjusted within the scope of matters; Again by air feedPipe upper port is connected with extraneous filtrated air source, ventilates, and starts to cultivate.
A kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts provided by the invention, canEnsure that dissolved oxygen abundance, light transmittance are high, are suitable for algal grown; Heat exchanger is placed in to the direct temperature of controlling in bottle,Neither can affect the efficiency of light energy utilization has also ensured temperature controlled accurate fast simultaneously; By set up spore in bottleThe behaviour that collector can be collected algae spore, greatly simplify traditional incubation extraction spore in incubationDo, be convenient to the further amplification that algae kind is cultivated; In addition, dismounting of the present invention is easy, is convenient to clean sterilization methodSimply, be particularly useful for the pure culture of macro.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
In figure:
1, blake bottle 2, plug 3, air supply pipe
4, wireway 5, tampon 6, gas distributor
7, heat exchanger 8, support bar 9, spore collector
Detailed description of the invention
Get blake bottle 1, plug 2, air supply pipe 3, wireway 4, tampon 5, gas distributor 6, heat exchangeDevice 7, support bar 8, spore collector 9, will be with upper-part heat sterilization, simultaneously by for algae cultureComplete and the sterilizing of culture medium preparation.
Parts complete above sterilizing, culture medium and algae kind to be cultivated are carried out to following company in gnotobasisConnect:
(1) by the bottleneck of plug 2 jam-pack blake bottles 1;
(2) air supply pipe 3 is also vertically inserted to blake bottle 1 inside in the middle of plug 2;
(3) gas distributor 6 be placed in to blake bottle 1 inside and be fixedly connected with air supply pipe 3 lower ends, and protectingCard gas distributor 6 lower ends to the distance at the bottom of 1 bottle, blake bottle be blake bottle 1 bottleneck to 1/3 of bottle end distance,Then by algae kind bondage to be cultivated in gas distributor periphery;
(4) heat exchanger 7 is also vertically inserted to blake bottle 1 inside in the middle of plug 2, and ensure itLower end is that blake bottle 1 bottleneck is to 1/6 of bottle end distance to the distance at the bottom of 1 bottle, blake bottle;
(5) support bar 8 is also vertically inserted to blake bottle 1 inside in the middle of plug 2, its lower end is arrivedDistance at the bottom of 1 bottle, blake bottle is that blake bottle 1 bottleneck is to 2/3 of bottle end distance
(6) spore collector 9 be placed in to blake bottle 1 inside and be fixedly connected with support bar 8 lower ends
(7) blake bottle 1 inside is also vertically inserted in wireway 4 one of them limit in the middle of plug 2,Make its lower end than support bar 8 lower ends high 5cm in vertical direction, meanwhile, its another limit and position, summit, right angleIn blake bottle 1 outside
(8) tampon 5 is placed in to the inside on above-mentioned wireway 4 another limits, the position of close port, is emptyCover shape.
After completing above Connection Step, the culture medium after sterilizing is joined in bottle, culture medium liquid level is arrivedThe distance at the bottle end be at the bottom of bottleneck arrives bottle distance 2/3; Then, utilize heat exchanger 7 by liquid environment temperature in bottleDegree is adjusted within the scope of matters; Again air supply pipe 3 upper port are connected with extraneous filtrated air source, lead toGas, starts to cultivate.
Above embodiments of the invention are had been described in detail, but described content is only better enforcement of the present inventionExample, in order to not limit the present invention. All any amendments of making, be equal to replacement in application range of the present inventionWith improve etc., within all should being included in protection scope of the present invention.
Claims (5)
1. with a controllable temperature macro suspension culture apparatus for Spore adhesion parts, comprise cultivationBottle (1), plug (2), air supply pipe (3), wireway (4), tampon (5), distribution of gasDevice (6), characterized by further comprising heat exchanger (7), support bar (8), spore collector (9),Wherein, the bottleneck of plug (2) jam-pack blake bottle (1); Air supply pipe (3) is in plug (2)Between and vertically insert blake bottle (1) inside; Wireway (4) is shape at right angles, and one of them limit is run throughIn the middle of plug (2) and vertically, insertion blake bottle (1) inside, another limit and summit, right angle are positioned atBlake bottle (1) outside; Tampon (5) is placed in the inside, close on another limit of above-mentioned wireway (4)The position of port; Gas distributor (6) be positioned at blake bottle (1) inner and with air supply pipe (3) lower endBe fixedly connected with; Heat exchanger (7) is in the middle of plug (2) and in vertical insertion blake bottle (1)Portion; Support bar (8) also vertically inserts blake bottle (1) inside in the middle of plug (2); SporeCollector (9) is positioned at blake bottle (1) inside and is fixedly connected with described support with support bar (8) lower endBar (8) lower end to the distance at blake bottle (1) the bottle end be blake bottle (1) bottleneck to 2/3 of bottle end distance,Described tampon (5) is is left unlocked or unlatched shape, does not block the gas flow in described wireway (4), described confessionBetween tracheae (3) and described gas distributor (6), there is gas interchange channel.
2. a kind of controllable temperature macro with Spore adhesion parts according to claim 1 suspendsCulture apparatus, is characterized in that described heat exchanger (7) lower end to the distance at blake bottle (1) the bottle end isBlake bottle (1) bottleneck is to 1/6 of bottle end distance.
3. a kind of controllable temperature macro with Spore adhesion parts according to claim 1 suspendsCulture apparatus, is characterized in that described gas distributor (6) lower end is to blake bottle (1) distance at the bottle endFor blake bottle (1) bottleneck is to 1/3 of bottle end distance.
4. a kind of controllable temperature macro with Spore adhesion parts according to claim 1 suspendsCulture apparatus, is characterized in that described wireway (4) lower end is higher than described support bar (8) lower end, withShi Suoshu wireway (4) lower end and described support bar (8) lower end distance are in vertical direction 3-5cm.
5. a kind of controllable temperature macro with Spore adhesion parts according to claim 1 suspendsCulture apparatus, the material that it is characterized in that described blake bottle (1) is glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310430971.5A CN103493732B (en) | 2013-09-18 | 2013-09-18 | A kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310430971.5A CN103493732B (en) | 2013-09-18 | 2013-09-18 | A kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts |
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| Publication Number | Publication Date |
|---|---|
| CN103493732A CN103493732A (en) | 2014-01-08 |
| CN103493732B true CN103493732B (en) | 2016-05-18 |
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| CN201310430971.5A Expired - Fee Related CN103493732B (en) | 2013-09-18 | 2013-09-18 | A kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766204B (en) * | 2014-01-20 | 2015-08-19 | 中国科学院东北地理与农业生态研究所 | A kind of device for the cultivation of wetland Algae preparation is utilized to cultivate the method for Algae preparation |
| CN105123527A (en) * | 2015-09-17 | 2015-12-09 | 山东省农作物种质资源中心 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
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|---|---|---|---|---|
| SU1669979A1 (en) * | 1988-06-28 | 1991-08-15 | Ставропольский сельскохозяйственный институт | Apparatus for cultivating microalgae |
| CN2234443Y (en) * | 1995-07-31 | 1996-09-04 | 缪坚人 | Spirulina photosynthesis reactor |
| CN2384948Y (en) * | 1999-06-11 | 2000-06-28 | 中国科学院化工冶金研究所 | Air lift closed photo-biological reactor |
| CN2473210Y (en) * | 2001-03-26 | 2002-01-23 | 中国科学院化工冶金研究所 | Cell cultivation and air float collection coupling reactor |
| CN2482797Y (en) * | 2001-06-15 | 2002-03-27 | 中国科学院化工冶金研究所 | Photo-bioreactor suitable for cultivating large kelp seedling |
| DE19912952C2 (en) * | 1999-03-23 | 2002-03-28 | Martina Von Ahn | Process for utilizing carbon dioxide from chemical processes and device for carrying out the same |
| CN2604440Y (en) * | 2003-01-27 | 2004-02-25 | 人宇生物科技股份有限公司 | Arrangement for producing high-absorbance rhodophyll with ceylon sea membrance |
| CN101935610A (en) * | 2010-08-04 | 2011-01-05 | 南京工业大学 | Multi-group bubbling type photobioreactor |
| CN203505236U (en) * | 2013-09-18 | 2014-04-02 | 天津海友佳音生物科技股份有限公司 | Temperature-controllable large-algae suspension culture device with spore attached component |
-
2013
- 2013-09-18 CN CN201310430971.5A patent/CN103493732B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1669979A1 (en) * | 1988-06-28 | 1991-08-15 | Ставропольский сельскохозяйственный институт | Apparatus for cultivating microalgae |
| CN2234443Y (en) * | 1995-07-31 | 1996-09-04 | 缪坚人 | Spirulina photosynthesis reactor |
| DE19912952C2 (en) * | 1999-03-23 | 2002-03-28 | Martina Von Ahn | Process for utilizing carbon dioxide from chemical processes and device for carrying out the same |
| CN2384948Y (en) * | 1999-06-11 | 2000-06-28 | 中国科学院化工冶金研究所 | Air lift closed photo-biological reactor |
| CN2473210Y (en) * | 2001-03-26 | 2002-01-23 | 中国科学院化工冶金研究所 | Cell cultivation and air float collection coupling reactor |
| CN2482797Y (en) * | 2001-06-15 | 2002-03-27 | 中国科学院化工冶金研究所 | Photo-bioreactor suitable for cultivating large kelp seedling |
| CN2604440Y (en) * | 2003-01-27 | 2004-02-25 | 人宇生物科技股份有限公司 | Arrangement for producing high-absorbance rhodophyll with ceylon sea membrance |
| CN101935610A (en) * | 2010-08-04 | 2011-01-05 | 南京工业大学 | Multi-group bubbling type photobioreactor |
| CN203505236U (en) * | 2013-09-18 | 2014-04-02 | 天津海友佳音生物科技股份有限公司 | Temperature-controllable large-algae suspension culture device with spore attached component |
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| CN103493732A (en) | 2014-01-08 |
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Granted publication date: 20160518 |