CN203505237U - Macrophytic alga closed type culture device with culture medium convenient to replace - Google Patents
Macrophytic alga closed type culture device with culture medium convenient to replace Download PDFInfo
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- CN203505237U CN203505237U CN201320582674.8U CN201320582674U CN203505237U CN 203505237 U CN203505237 U CN 203505237U CN 201320582674 U CN201320582674 U CN 201320582674U CN 203505237 U CN203505237 U CN 203505237U
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- bottle
- blake bottle
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The utility model provides a macrophytic alga closed type culture device with a culture medium convenient to replace. The macrophytic alga closed type culture device comprises a culture bottle, a rubber plug, a gas supply pipe, a gas guide pipe, a cotton plug, a gas distributor, a heat exchanger, a supporting rod, a spore collector, a liquid adding mouth, a liquid discharge mouth, a liquid adding valve and a liquid discharge valve. The device has the advantages of being high in transparency and sufficient in supplied oxygen, enabling temperature control to be convenient, enabling spore collection to be convenient and easy, enabling the culture medium to be replaced without dismounting and mounting the device and the like, and a proper environment can be provided for macrophytic alga culture. Meanwhile, the device is simple in structure, convenient to clean, simple in sterilization method and especially suitable for the pure culture of macrophytic algae.
Description
Technical field
The utility model relates to algae culture technical field, relates in particular to a kind of closed culture apparatus of macro of being convenient to change medium.
Background technology
Large-scale algae culture technique be by a small amount of algae kind in appropriate incubation device by efficient culture technology, realize the process of frond growth, propagation.
Existing macro culture technique can be divided into open cultivation and closed cultivation two classes, and wherein open cultivation is carried out in environment conventionally in the open, and frond and medium directly contact with external environment.This cultural method is relatively extensive, and environmental condition control accuracy is lower, is mainly used in product purity is required to lower purposes.And closed cultivation typically refers to the culture medium inoculated algae kind of utilizing in closed environment after sterilizing, cultivate, can effectively guarantee to cultivate purity and precision, the purposes that is applicable to product purity to have relatively high expectations.
The closed culture apparatus of algae of prior art, its temperature is controlled and generally by ambient temperature, is regulated or realize at culture apparatus outer setting heat exchange chuck, but all there is obvious defect in these two kinds of methods: the former temperature regulates slowly, energy consumption is large, and the latter affects light transmittance.In addition, the closed culture apparatus of the algae of prior art does not generally have spore collecting function, and the operation that therefore causes obtaining macro spore in incubation is more difficult.Meanwhile, the closed culture apparatus of algae of prior art, the defect of medium is changed in ubiquity inconvenience, after accessing first medium and algae kind, along with the growth metabolism of frond can produce a large amount of harmful metabolites, cause culture environment to worsen, suppress algal grown, therefore in incubation, should regularly replace medium.But because the structure of culture apparatus is limit, need after many parts dismountings, to carry out in the process of changing medium, complex operation step, easily causes microbiological contamination on the other hand so on the one hand again.
Summary of the invention
The utility model, for the technological deficiency of prior art, provides a kind of closed culture apparatus of macro of being convenient to change medium.
For realizing above technical purpose, the utility model is taked following technical scheme.
A kind of closed culture apparatus of macro of being convenient to change medium, comprise blake bottle, plug, air supply pipe, wireway, tampon, gas distributor, heat exchanger, support bar, spore collector, filling opening, leakage fluid dram, charging valve, tapping valve, wherein, the bottleneck of plug jam-pack blake bottle; Insertion blake bottle is inner in the middle of plug and vertically for air supply pipe; The rectangular shape of wireway, in the middle of plug and vertically, insertion blake bottle inside, another limit and summit, right angle are positioned at blake bottle outside on one of them limit; Tampon is placed in the inside on another limit of above-mentioned wireway, the position of close port; Gas distributor is positioned at blake bottle inside and is fixedly connected with air supply pipe lower end; Insertion blake bottle is inner in the middle of plug and vertically for heat exchanger; Insertion blake bottle is inner in the middle of plug and vertically for support bar; Spore collector is positioned at blake bottle inside and is fixedly connected with support bar lower end; Filling opening is positioned at the side of blake bottle and is connected with charging valve; Leakage fluid dram is positioned at the side of blake bottle and is connected with tapping valve.
In said structure, heat exchanger lower end is that blake bottle bottleneck is to 1/6 of bottle end distance to the distance at the bottom of blake bottle bottle; Described gas distributor lower end is that blake bottle bottleneck is to 1/3 of bottle end distance to the distance at the bottom of blake bottle bottle; Described support bar lower end is that blake bottle bottleneck is to 2/3 of bottle end distance to the distance at the bottom of blake bottle bottle; Described wireway lower end is higher than described support bar lower end, and described wireway lower end and described support bar lower end distance are in vertical direction 3-5cm simultaneously; Described filling opening is greater than blake bottle bottleneck to 2/3 of bottle end distance to the distance at the bottom of blake bottle bottle; Described leakage fluid dram is less than 2cm to the distance at the bottom of blake bottle bottle;
This device in use, first above-mentioned all parts are carried out to sterilization treatment, then in gnotobasis, all parts are connected complete according to said structure feature, again by algae kind bondage to be cultivated in gas distributor periphery, again the medium after sterilizing is joined in bottle, make medium liquid level to the distance at the bottle end be at the bottom of bottleneck arrives bottle distance 2/3; Then, utilize heat exchanger that liquid environment temperature in bottle is adjusted within the scope of matters; Again air supply pipe upper port is connected with extraneous filtrated air source, ventilates, start to cultivate.
In incubation, when needs are changed medium, open tapping valve a bottle interior medium is flowed out, close tapping valve; Open again charging valve by configured in advance the fresh culture well and after sterilizing by filling opening join in blake bottle, make medium liquid level to the distance at bottle end be at the bottom of bottleneck arrives bottle distance 2/3, close charging valve.
A kind of closed culture apparatus of macro of being convenient to change medium that the utility model provides, to possess that high transmission rate, oxygen supply are sufficient, temperature is controlled convenient simultaneously, collect spore easy, without dismounting, be the advantageous feature such as replaceable medium, can cultivate adapt circumstance is provided for macro.Meanwhile, this apparatus structure is simple, be convenient to clean, and sterilization method is simple, is particularly useful for the pure culture of macro.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
In figure:
1, blake bottle 2, plug 3, air supply pipe
4, wireway 5, tampon 6, gas distributor
7, heat exchanger 8, support bar 9, spore collector
10, filling opening 11, leakage fluid dram 12, charging valve
13, tapping valve
Embodiment
Get the blake bottle 1, plug 2, air supply pipe 3, wireway 4, tampon 5, gas distributor 6, heat exchanger 7, support bar 8, spore collector 9, charging valve 12, the tapping valve 13 that offer filling opening 10 and leakage fluid dram 11 simultaneously, will be with upper-part heat sterilization, simultaneously by the complete and sterilizing of medium preparation for algae culture.
Parts, medium and algae kind to be cultivated that above sterilizing is complete are carried out to following connection in gnotobasis:
(1) by the bottleneck of plug 2 jam-pack blake bottles 1;
(2) air supply pipe 3 is also vertically inserted to blake bottle 1 inside in the middle of plug 2;
(3) gas distributor 6 be placed in to blake bottle 1 inside and be fixedly connected with air supply pipe 3 lower ends, and guarantee gas distributor 6 lower ends to the distance at the bottom of 1 bottle, blake bottle be blake bottle 1 bottleneck to 1/3 of bottle end distance, then by algae kind bondage to be cultivated in gas distributor periphery;
(4) by heat exchanger 7 in the middle of plug 2 and vertically insert blake bottle 1 inside, and guarantee that its lower end is that blake bottle 1 bottleneck is to 1/6 of bottle end distance to the distance at the bottom of 1 bottle, blake bottle;
(5) by support bar 8 in the middle of plug 2 and vertically insert blake bottle 1 inside, making its lower end is that blake bottle 1 bottleneck is to 2/3 of bottle end distance to the distance at the bottom of 1 bottle, blake bottle
(6) spore collector 9 be placed in to blake bottle 1 inside and be fixedly connected with support bar 8 lower ends
(7) blake bottle 1 inside is also vertically inserted in wireway 4 one of them limit in the middle of plug 2, guarantee that its lower end is in vertical direction than the support bar 8 high 4cm in lower end, meanwhile, its another limit and summit, right angle are positioned at blake bottle 1 outside
(8) tampon 5 is placed in to the inside on above-mentioned wireway 4 another limits, the position of close port.
(9) charging valve (12) is connected in to filling opening (10) upper, makes the unlatching by charging valve or close and can control opening or sealing of filling opening.
(10) tapping valve (13) is connected in to leakage fluid dram (11) upper, makes the unlatching by charging valve or close and can control opening or sealing of filling opening.
After completing above Connection Step, the medium after sterilizing is joined in bottle, make medium liquid level to the distance at the bottle end be at the bottom of bottleneck arrives bottle distance 2/3; Then, utilize heat exchanger 7 that liquid environment temperature in bottle is adjusted within the scope of matters; Again air supply pipe 3 upper port are connected with extraneous filtrated air source, ventilate, start to cultivate.
Cultivate after 5 days and find that medium needs to change, now open tapping valve 13 a bottle interior medium is flowed out, close tapping valve 13; Open again charging valve 12 by configured in advance the fresh culture well and after sterilizing by filling opening 10 join in blake bottle, make medium liquid level to the distance at the bottle end be at the bottom of bottleneck arrives bottle distance 2/3, close charging valve, complete medium replacing.
Above embodiment of the present utility model is had been described in detail, but described content is only preferred embodiment of the present utility model, not in order to limit the utility model.All any modifications of making in application range of the present utility model, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (7)
1. the closed culture apparatus of macro of being convenient to change medium, comprise blake bottle (1), plug (2), air supply pipe (3), wireway (4), tampon (5), gas distributor (6), heat exchanger (7), support bar (8), spore collector (9), characterized by further comprising filling opening (10), leakage fluid dram (11), charging valve (12), tapping valve (13), wherein, the bottleneck of plug (2) jam-pack blake bottle (1); Air supply pipe (3) also vertically inserts blake bottle (1) inside in the middle of plug (2); The rectangular shape of wireway (4), in the middle of plug (2) and vertically, insertion blake bottle (1) inside, another limit and summit, right angle are positioned at blake bottle (1) outside on one of them limit; Tampon (5) is placed in the inside on another limit of above-mentioned wireway (4), the position of close port; Gas distributor (6) is positioned at blake bottle (1) inside and is fixedly connected with air supply pipe (3) lower end; Heat exchanger (7) also vertically inserts blake bottle (1) inside in the middle of plug (2); Support bar (8) also vertically inserts blake bottle (1) inside in the middle of plug (2); Spore collector (9) is positioned at blake bottle (1) inside and is fixedly connected with support bar (8) lower end; Filling opening (10) is positioned at the side of blake bottle (1) and is connected with charging valve (12); Leakage fluid dram (11) is positioned at the side of blake bottle (1) and is connected with tapping valve (13).
2. a kind of closed culture apparatus of macro of being convenient to change medium according to claim 1, is characterized in that described heat exchanger (7) lower end is that blake bottle (1) bottleneck is to 1/6 of bottle end distance to the distance at blake bottle (1) the bottle end.
3. a kind of closed culture apparatus of macro of being convenient to change medium according to claim 1, is characterized in that described gas distributor (6) lower end is that blake bottle (1) bottleneck is to 1/3 of bottle end distance to the distance at blake bottle (1) the bottle end.
4. a kind of closed culture apparatus of macro of being convenient to change medium according to claim 1, is characterized in that described support bar (8) lower end is that blake bottle (1) bottleneck is to 2/3 of bottle end distance to the distance at blake bottle (1) the bottle end.
5. a kind of closed culture apparatus of macro of being convenient to change medium according to claim 1, it is characterized in that described wireway (4) lower end is higher than described support bar (8) lower end, described wireway (4) lower end and described support bar (8) lower end distance are in vertical direction 3-5cm simultaneously.
6. a kind of closed culture apparatus of macro of being convenient to change medium according to claim 1, is characterized in that described filling opening (10) is more than or equal to blake bottle (1) bottleneck to 2/3 of bottle end distance to the distance at blake bottle (1) the bottle end.
7. a kind of closed culture apparatus of macro of being convenient to change medium according to claim 1, is characterized in that described leakage fluid dram (11) is less than 2cm to the distance at blake bottle (1) the bottle end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320582674.8U CN203505237U (en) | 2013-09-18 | 2013-09-18 | Macrophytic alga closed type culture device with culture medium convenient to replace |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320582674.8U CN203505237U (en) | 2013-09-18 | 2013-09-18 | Macrophytic alga closed type culture device with culture medium convenient to replace |
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| Publication Number | Publication Date |
|---|---|
| CN203505237U true CN203505237U (en) | 2014-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320582674.8U Withdrawn - After Issue CN203505237U (en) | 2013-09-18 | 2013-09-18 | Macrophytic alga closed type culture device with culture medium convenient to replace |
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| CN (1) | CN203505237U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477969A (en) * | 2013-09-18 | 2014-01-01 | 天津海友佳音生物科技股份有限公司 | Macro-algae closed culture device facilitating culture medium replacement |
-
2013
- 2013-09-18 CN CN201320582674.8U patent/CN203505237U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477969A (en) * | 2013-09-18 | 2014-01-01 | 天津海友佳音生物科技股份有限公司 | Macro-algae closed culture device facilitating culture medium replacement |
| CN103477969B (en) * | 2013-09-18 | 2014-12-17 | 天津海友佳音生物科技股份有限公司 | Macro-algae closed culture device facilitating culture medium replacement |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140402 Effective date of abandoning: 20141217 |
|
| RGAV | Abandon patent right to avoid regrant |