CN103125269A - Simple fermentation device for liquid spawn submerged cultivation - Google Patents

Simple fermentation device for liquid spawn submerged cultivation Download PDF

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Publication number
CN103125269A
CN103125269A CN 201210570858 CN201210570858A CN103125269A CN 103125269 A CN103125269 A CN 103125269A CN 201210570858 CN201210570858 CN 201210570858 CN 201210570858 A CN201210570858 A CN 201210570858A CN 103125269 A CN103125269 A CN 103125269A
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China
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temperature
filter
bottle
control instrument
temperature control
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CN 201210570858
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Chinese (zh)
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何寒
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何寒
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Abstract

The invention relates to a simple fermentation device for liquid spawn submerged cultivation. The simple fermentation device is structurally composed of a constant-temperature pond, a cultivation bottle and an air pump, wherein the constant-temperature pond contains clear water, and the cultivation bottle is placed inside the constant-temperature bottle. The constant-temperature pond is composed of a temperature-control instrument, a heating pipe and a temperature probe, the cultivation bottle is composed of a bottle plug, an air inlet pipe and a filter, the air pump is connected with the air inlet pipe through the filter and a rubber pipe, oxygen is conveyed into the cultivation bottle through the air pump, and mycelia (mycelium pellet) inside liquid spawn cultivated inside the cultivation bottle metabolize and differentiate continuously through stirring of the oxygen to become more and more. The heating pipe heats the water inside the constant-temperature pond and keeps the water temperature within a certain range under control of the temperature-control instrument, the liquid spawn inside the cultivation bottle is cultivated in the optimum temperature environment, the cultivation period of the spawn is short, and quality of the spawn is good. The simple fermentation device is small in investment, takes effect quickly, is simple and convenient to operate, and is very suitable for being applied to small-size edible mushroom factories, individual households, and rural families.

Description

A kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting
Technical field
The invention belongs to a kind of device of producing edible fungi liquid strain, be specifically related to a kind of liquid strain in depth layer and cultivate simple and easy installation for fermenting.
Background technology
That edible fungi liquid strain has is with short production cycle, the cell age neat and consistent, inoculation is convenient, fermentation is fast, be suitable for the advantage such as batch production production, and its China's Edible Fungi that thoroughly is through with has been continued to use more than one thousand years and adopted the history of solid spawn, is a qualitative leap.At present, mainly contain two kinds of shaking table and fermentation tanks for the production of the edible fungi liquid strain installations and facilities.Fermentation tank not only involves great expense, but also it is numerous and diverse to have application process, high, the shortcoming such as have a big risk that consumes energy, and general mushroom agriculture is difficult to bear, when shaking table is produced liquid spawn, because the reasons in structure of equipment has limited its output.
Application number be 89203999.X's " Simple liquid strain production device ", this utility model is characterised in that this device has ultra-clean filter, inoculator and multistage air cleaner, all adopts the closure pipeline to produce.This utility model culture fluid used does not need whole autoclavings, just can carry out closed continuous culture.
Application number is 94216524.1 " without the shaking-table edible liquid bacteria fertilizer ", and this this device comprises that primary fermentation culture apparatus, middle rank enlarge the fermented and cultured device and whole level enlarges the fermented and cultured device.The basic structure form of installation for fermentings at different levels is: air-breather is connected with air cleaner by flexible pipe, and filter is connected with breather pipe by flexible pipe, and breather pipe fills by the bottleneck cotton and inserts in blake bottle.And the filter that middle rank and whole level enlarge the fermented and cultured device is connected with barometric pipe by flexible pipe and three-way pipe, and communicates with flow diverter.
Application number is 87210089 " Simple apparatus for culturing liquid strain in depth layer ", this device is by easy air bag or the stingy pump air feed of electromagnetism, ventilation head, short hard tube and flexible pipe ventilation, tolerance adjustable filtering device is that installation for fermenting forms with the bulb filter filter gas of filling common cotton and with simple and easy fermenter.It has substituted without oil gas press, moisture trap, metal-air filter and glass fermentation flask, got rid of shaking table, cost is reduced to hundred yuan of left and right greatly, when operation operation not necessarily needs power supply and autoclave sterilization autoclave, and this provides for vast rural area edible mushroom specialist or open-air microorganism scientific research simple and easy reliable strain cultivation and the process units of affording, can use.
Application number is 200920244525.4 " Simple liquid strain fermenter ", this utility model mainly by: oil-less pump, first, second and third sebific duct, first, second, third and fourth filter ball, first, second and third breather pipe, first and second cylinder of steel, first and second filler, exhaust outlet, connect bacterium mouth, fermentation tank, connecting plate and consist of.The first filter ball one end is connected with oil-less pump, the other end is connected with the first cylinder of steel, the second filter ball one end is connected with the first cylinder of steel, the other end is connected with the second cylinder of steel, the 3rd filter ball one end is connected with the second cylinder of steel, the other end is connected with the 3rd breather pipe by the 4th sebific duct, the 4th filter ball, and the 3rd breather pipe is connected with fermentation tank, exhaust outlet is housed on fermentation tank and connects the bacterium mouth.
Application number is 200820160881.3 " a kind of Simple device for culturing liquid strain ", and this device is by vial (1), rubber stopper (2), and glass tube (3), glass bulb (4) and connecting hose (5) form.It is characterized in that: the diameter at butt end of rubber stopper (2) is larger than glass bottle opening, upper 2 holes, insertion glass tube (3) and the glass bulb (4) of making a call to of rubber stopper (2).Glass tube (3) is air inlet pipe, and length is a little more than the height of vial, and the upper end connects glass bulb (4) with silicone tube (5), and rubber stopper is passed in the lower end, port at the bottom of the bottle 1-2 centimetre.Fill with clean cotton in glass bulb (4), so that filtered air.
application number is 200520079434.1 " Simplified fermentor for producing liquid spawn ", this device is to adopt saline bottle to make round, cover has rubber ring on the bottleneck convex edge of saline bottle, rubber ring has tapped ferrule at the hypomere cover of bottleneck convex edge, in-built filter medium and with externally threaded crown cap cover on bottleneck and the precession ferrule in to bottleneck convex edge shape up and down altogether the gesture of pressure to reach the purpose to bottle sealing, side wall upper part at crown cap is equipped with an outlet nozzle, the admission line that is connected with air cleaner passes the through bottle of the circular hole end of crown cap central authorities.This utility model compact conformation, easy to use, be beneficial to the storing of liquid spawn.
Characteristics such as that above-mentioned several patent all has is simple in structure, application cost is low, practical, but all do not dispose thermostat, bacterial classification cause at the easy temperature influence of incubation that the strain cultivation cycle is different in size, strain quality is also unstable.
Summary of the invention
Purpose of the present invention is exactly to overcome the deficiency of the said equipment facility and provide brand-new a kind of liquid strain in depth layer to cultivate simple and easy installation for fermenting.Specifically comprise: the structure of this device mainly is comprised of temperature bath, blake bottle, air pump; Wherein, temperature bath is equipped with clear water, and blake bottle is to be placed in temperature bath; Temperature bath is comprised of temperature control instrument, heating tube, temp probe, blake bottle is comprised of bottle stopper, air inlet pipe, filter, air pump is to link together by filter, sebific duct and air inlet pipe, toward blake bottle input oxygen, constantly metabolism and dissociation must get more and more the mycelium (ball) in the liquid spawn of cultivating in bottle under the stirring of oxygen by air pump; Control heating tube by temperature control instrument and the water of temperature bath the inside is heated and keep water temperature must scope, in blake bottle, liquid spawn be cultivated under the temperature environment of the best, and the bacterial classification cultivation cycle is short, the quality high-quality.
The present invention is achieved through the following technical solutions:
1, a kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting, and in technical scheme, the structure of this device mainly is comprised of temperature bath (1), blake bottle (2), air pump (3); Wherein, temperature bath (1) is equipped with clear water, and blake bottle (2) is to be placed in temperature bath (1); Temperature bath (1) is comprised of temperature control instrument (4), heating tube (5), temp probe (6), heating tube (5) and temp probe (6) are all to be placed in temperature bath (1), and are all to connect temperature control instrument (4); Blake bottle (2) is comprised of bottle stopper (7), air inlet pipe (8), filter (9), air inlet pipe (8) and filter (9) are all to penetrate and be placed in bottle stopper (7), and bottle stopper (7) is placed in the bottleneck of blake bottle (2); Air pump (3) is to link together with air inlet pipe (8) by filter (10) and sebific duct (11); Filter (9) the inside is placed with cotton (12), is placed with cotton (12) and active carbon (13) inside filter (10).
2, described a kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting, and the temperature control instrument in technical scheme (4) and heating tube (5) are all commercially available, relation and the effect of temperature control instrument (4) and heating tube (5) are: by giving temperature control instrument (4), certain temperature parameter is set, the water temperature that detects temperature bath (1) the inside when the temp probe that is placed in temperature bath (1) (6) is during lower than the set temperature parameter of temperature control instrument (4), temperature control instrument (4) starts heating tube (5), heating tube (5) heating of feeding water immediately, when the set temperature parameter of the water temperature inside temperature bath (1) and temperature control instrument (4) conforms to, temperature control instrument (4) is closed heating tube (5) stopped heating, thereby reach the water temperature that keeps temperature bath (1) the inside in the purpose of certain limit.
3, described a kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting, and the blake bottle in technical scheme (2) is the commercial goods, and its shape is cylinder shape, can bear in high temperature, high-pressure bottle and sterilize.
4, described a kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting, air pump in technical scheme (3) is the commercial goods, input oxygen by air pump toward blake bottle (2), mycelium (ball) in the liquid spawn of cultivating in bottle under the stirring of oxygen constantly metabolism and dissociation must get more and more, finally turn out the liquid spawn of high-quality.
5, described a kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting, and the filter in technical scheme (9) and filter (10) are all commercially available ball-type glass tubes, can bear high temperature, autoclave sterilization.
6, described a kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting, and the active carbon (13) that is placed on filter (10) in technical scheme is graininess.
7, described a kind of liquid strain in depth layer is cultivated simple and easy installation for fermenting, and the sebific duct (11) that is used for connection air pump (3), filter (10) and air inlet pipe (8) in technical scheme is the commercial goods, can bear high temperature, autoclave sterilization.
Compared with prior art, the invention has the beneficial effects as follows:
1, the present invention is simple in structure, but design science is reasonable, and not only cost is low, easy to use, and can replace traditional liquid spawn production facilities and equipments such as big-and-middle-sized fermentation tube, shaking table to produce liquid spawn fully.
When 2, the present invention uses, controlling heating tube by temperature control instrument heats the water of temperature bath the inside and keeps water temperature must scope, blake bottle is placed in temperature bath, and making bottle liquid spawn of the inside is to cultivate under the temperature environment of the best, and the bacterial classification cultivation cycle is short, the quality high-quality.
3, the glass marble that application cost of the present invention is cheap is as filter, and the filtration system volume is little, is placed with granular active carbon at the filter of breather line setting, can adsorb the air humidity that circulates in filter, makes the filter quality of filter more superior.
Little power consumption when 4, the present invention uses, success rate is high, and is good in economic efficiency, reduced production cost.
5, small investment of the present invention, instant effect, easy and simple to handle not only are suitable for small-sized edible mushroom factory and individual household with specialized lines of work use, but also can be in rural households' wide popularization and application.
Description of drawings
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is structural representation of the present invention.
In Fig. 1, the 1st, temperature bath, the 2nd, blake bottle, the 3rd, air pump, the 4th, temperature control instrument, the 5th, heating tube, the 6th, temp probe, the 7th, bottle stopper, the 8th, air inlet pipe, the 9th, blake bottle filter, the 10th, piping filter, the 11st, sebific duct, the 12nd, cotton, the 13rd, active carbon, the 14th, temp probe wire, the 15th, heating tube power line, the 16th, the power line of temperature control instrument, the 17th, the power line of air pump.
Embodiment
Now the present invention will be described in detail by reference to the accompanying drawings:
The present invention is comprised of temperature bath (1), blake bottle (2), air pump (3).
in Fig. 1, the 1st, temperature bath (1), the 2nd, blake bottle (2), the 3rd, air pump (3), the 4th, temperature control instrument (4), the 5th, heating tube (5), the 6th, temp probe (6), the 7th, bottle stopper (7), the 8th, air inlet pipe (8), the 9th, blake bottle filter (9), the 10th, piping filter (10), the 11st, sebific duct (11), the 12nd, cotton (12), the 13rd, active carbon (13), the 14th, temp probe wire (14), the 15th, heating tube power line (15), the 16th, the power line of temperature control instrument (16), the 17th, the power line of air pump (17).Wherein, temperature bath (1) is equipped with clear water, and blake bottle (2) is to be placed in temperature bath (1); Temperature bath (1) is comprised of temperature control instrument (4), heating tube (5), temp probe (6), heating tube (5) and temp probe (6) are all to be placed in temperature bath (1), and are all to connect temperature control instrument (4); Blake bottle (2) is comprised of bottle stopper (7), air inlet pipe (8), filter (9), air inlet pipe (8) and filter (9) are all to penetrate and be placed in bottle stopper (7), and bottle stopper (7) is placed in the bottleneck of blake bottle (2); Air pump (3) is to link together with air inlet pipe (8) by filter (10) and sebific duct (11); What placed filter (9) the inside is cotton (12), and what place inside filter (10) is cotton (12) and active carbon (13).
relation and the effect of temperature control instrument (4) and heating tube (5) are: by giving temperature control instrument (4), certain temperature parameter is set, the water temperature that detects temperature bath (1) the inside when the temp probe that is placed in temperature bath (1) (6) is during lower than the set temperature parameter of temperature control instrument (4), temperature control instrument (4) starts heating tube (5), heating tube (5) heating of feeding water immediately, when the set temperature parameter of the water temperature inside temperature bath (1) and temperature control instrument (4) conforms to, temperature control instrument (4) is closed heating tube (5) stopped heating, thereby reach the water temperature that keeps temperature bath (1) the inside in the purpose of certain limit.
The shape of blake bottle (2) is cylinder shape, can bear in high temperature, high-pressure bottle and sterilize.
The effect of air pump is: by air pump toward blake bottle (2) input oxygen, the mycelium (ball) in the liquid spawn of cultivating in bottle under the stirring of oxygen constantly metabolism and dissociation must get more and more, finally turn out the liquid spawn of high-quality.
Filter (9) and filter (10) are all the ball-type glass tubes that can bear high temperature, autoclave sterilization.Place granular active carbon (13) at filter (10), the effect of active carbon (13) is: can adsorb the air humidity that circulates in filter, make the filter quality of filter more superior.
The sebific duct (11) that is used for connection air pump (3), filter (10) and air inlet pipe (8) can bear high temperature, autoclave sterilization.
Application method of the present invention and process: the liquid nutrient medium for preparing is sub-packed in to carry out sterilization in blake bottle (2) standby.Then filter (10) and sebific duct (11) are coupled together with air inlet pipe (8), and and bottle stopper (7) combine and form the filtration system of ventilating, it is standby that filtration system is passed through sterilization equally.Bacterial classification is accessed in blake bottle (2) under gnotobasis, filtration system is fit into equally in the bottleneck of blake bottle (2) under gnotobasis, then blake bottle (2) is positioned in temperature bath (1), set the temperature control instrument temperature parameter, start temperature control instrument the inner clear water of temperature bath (1) is carried out temperature control, simultaneously sebific duct (11) and air pump (2) are coupled together, booster air pump, toward blake bottle (2) delivering oxygen, bacterial classification to access is cultivated, and output is many, the liquid spawn of high-quality until cultivate.

Claims (7)

1. a liquid strain in depth layer is cultivated simple and easy installation for fermenting, and it is characterized in that: the structure of this device mainly is comprised of temperature bath (1), blake bottle (2), air pump (3); Wherein, temperature bath (1) is equipped with clear water, and blake bottle (2) is to be placed in temperature bath (1); Temperature bath (1) is comprised of temperature control instrument (4), heating tube (5), temp probe (6), heating tube (5) and temp probe (6) are all to be placed in temperature bath (1), and are all to connect temperature control instrument (4); Blake bottle (2) is comprised of bottle stopper (7), air inlet pipe (8), filter (9), air inlet pipe (8) and filter (9) are all to penetrate and be placed in bottle stopper (7), and bottle stopper (7) is placed in the bottleneck of blake bottle (2); Air pump (3) is to link together with air inlet pipe (8) by filter (10) and sebific duct (11); Filter (9) the inside is placed with cotton (12), is placed with cotton (12) and active carbon (13) inside filter (10).
2. a kind of liquid strain in depth layer described in is according to claim 1 cultivated simple and easy installation for fermenting, it is characterized in that described temperature control instrument (4) and heating tube (5) are all commercially available, relation and the effect of temperature control instrument (4) and heating tube (5) are: by giving temperature control instrument (4), certain temperature parameter is set, the water temperature that detects temperature bath (1) the inside when the temp probe that is placed in temperature bath (1) (6) is during lower than the set temperature parameter of temperature control instrument (4), temperature control instrument (4) starts heating tube (5), heating tube (5) heating of feeding water immediately, when the set temperature parameter of the water temperature inside temperature bath (1) and temperature control instrument (4) conforms to, temperature control instrument (4) is closed heating tube (5) stopped heating, thereby reach the water temperature that keeps temperature bath (1) the inside in the purpose of certain limit.
3. a kind of liquid strain in depth layer described in is according to claim 1 cultivated simple and easy installation for fermenting, and it is characterized in that: blake bottle (2) is the commercial goods, and its shape is cylinder shape, can bear in high temperature, high-pressure bottle and sterilize.
4. a kind of liquid strain in depth layer described in is according to claim 1 cultivated simple and easy installation for fermenting, it is characterized in that: air pump (3) is the commercial goods, input oxygen by air pump toward blake bottle (2), mycelium (ball) in the liquid spawn of cultivating in bottle under the stirring of oxygen constantly metabolism and dissociation must get more and more, finally turn out the liquid spawn of high-quality.
5. a kind of liquid strain in depth layer described in is according to claim 1 cultivated simple and easy installation for fermenting, and it is characterized in that: filter (9) and filter (10) are all commercially available ball-type glass tubes, can bear high temperature, autoclave sterilization.
6. a kind of liquid strain in depth layer described in is according to claim 1 cultivated simple and easy installation for fermenting, it is characterized in that: the active carbon (13) that is placed on filter (10) is graininess.
7. a kind of liquid strain in depth layer described in is according to claim 1 cultivated simple and easy installation for fermenting, it is characterized in that: the sebific duct (11) that is used for connection air pump (3), filter (10) and air inlet pipe (8) is the commercial goods, can bear high temperature, autoclave sterilization.
CN 201210570858 2012-12-25 2012-12-25 Simple fermentation device for liquid spawn submerged cultivation Pending CN103125269A (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105199964A (en) * 2015-08-18 2015-12-30 何翔 Oxygen-pumped and automatic constant-temperature liquid strain fermenting device
CN107548890A (en) * 2017-10-24 2018-01-09 翔天农业开发集团股份有限公司 The liquid cultivating method of mushroom

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105199964A (en) * 2015-08-18 2015-12-30 何翔 Oxygen-pumped and automatic constant-temperature liquid strain fermenting device
CN107548890A (en) * 2017-10-24 2018-01-09 翔天农业开发集团股份有限公司 The liquid cultivating method of mushroom

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Application publication date: 20130605