CN103224882A - Disposable wave type bioreactor with aerating apparatus - Google Patents
Disposable wave type bioreactor with aerating apparatus Download PDFInfo
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- CN103224882A CN103224882A CN2012100217226A CN201210021722A CN103224882A CN 103224882 A CN103224882 A CN 103224882A CN 2012100217226 A CN2012100217226 A CN 2012100217226A CN 201210021722 A CN201210021722 A CN 201210021722A CN 103224882 A CN103224882 A CN 103224882A
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- disposable wave
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- 238000005273 aeration Methods 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 11
- 238000011081 inoculation Methods 0.000 claims abstract description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 238000004113 cell culture Methods 0.000 abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 16
- 239000007789 gas Substances 0.000 abstract description 16
- 239000001301 oxygen Substances 0.000 abstract description 16
- 229910052760 oxygen Inorganic materials 0.000 abstract description 16
- 230000036284 oxygen consumption Effects 0.000 abstract description 9
- 238000012258 culturing Methods 0.000 abstract description 7
- 230000010261 cell growth Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 230000003321 amplification Effects 0.000 abstract description 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 2
- 238000009434 installation Methods 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 description 29
- 241000219095 Vitis Species 0.000 description 13
- 235000009754 Vitis X bourquina Nutrition 0.000 description 13
- 235000012333 Vitis X labruscana Nutrition 0.000 description 13
- 235000014787 Vitis vinifera Nutrition 0.000 description 13
- 238000004114 suspension culture Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000010008 shearing Methods 0.000 description 3
- 229930014669 anthocyanidin Natural products 0.000 description 2
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 2
- 235000008758 anthocyanidins Nutrition 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000010907 mechanical stirring Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention discloses a disposable wave type bioreactor with an aerating apparatus, which comprises a bag body, an inoculation port, a medium feed inlet, an air inlet, an air outlet, a sample port, a sensor intervention port and a discharge hatch are arranged on the bag body, a wave making device is provided at the lower part of the bag body, 1-40 installation interfaces are provided on the wall of the bag body, a gas joint with an air inlet pipe is connected to a sterilizing filter through a pipeline, and is passed through the installation interface for connecting to an aeration head arranged in the bag body through the pipeline. The reactor of the present invention can provide good air feeding for cell growth. The reactor can provide sufficient oxygen supply to the system with high oxygen consumption rate, high culture cell density and high culture viscosity during a process of culturing cell, and is in favor of realizing large-scale amplification and industrialization of high oxygen consumption cell culturing, and the gas cost is reduced. For the cell culture with low oxygen consumption requirement, the effective culture volume of the reactor is increased, and the culture cost is greatly reduced.
Description
Technical field
The invention belongs to disposable wave bio-reactor field, more specifically, the present invention relates to a kind of disposable wave bio-reactor that has aerating apparatus.
Background technology
Disposable wave bio-reactor is the equipment that various kinds of cell is cultivated that can be used for that has grown up since 1996.Utilize mechanical stirring or air lift type to realize mixing and the oxygen supply pair cell higher shearing force of generation and the cell injury that causes at classical pot type bio-reactor, disposable wave bio-reactor has been abandoned mechanical stirring and air lift type and has been mixed on principle of design, be particularly useful for mixing in the culturing process and the cell cultures of the shearing force sensitivity that ventilation is produced.This reactor places aseptic disposable cell culture bags with cell and nutrient solution, is placed on shaking on the platform of particular design.Shaking of platform produces wave in nutrient solution.Replace stirring and gas lift to realize the uniform mixing of culture by wave, make gas realize the oxygen transmission by wave by contacting with the surface of culture to upgrade, thereby produce the culture environment of a low-shearing power and relative high oxygen transfer efficiency, realize the high-efficient culture growth of cell and the production of target product.Finished cleanings, sterilization before simultaneously the wave bio-reactor is purchased and authenticated, the disposable use of user, this makes operation very light and avoided crossed contamination.In disposable wave bio-reactor, the generation of wave can make the nutrient solution thorough mixing and nutrient solution is upgraded by gas-liquid surface and contain the needed gas of cell, and the very soft adherent dependent form cell that can cultivate on the various microcarriers of this mixing.Wave motion can prevent partly that precipitation from producing and provide dissolved oxygen not producing under the foam.
The application of disposable wave bio-reactor is compared with the pot type bio-reactor and has been reduced the initial investment of the latter; Considerable expense can really saved aspect labor force, equipment, plant designing and the checking.They totally, promptly buy promptly with, do not need sterilization, therefore also just reduced demand as wash water system (WFI) and steam generator examining and repairing.Disposable wave bio-reactor does not need follow-up operation, has eliminated the possibility of crossed contamination between the technical process operation.Because reduce or abandoned demand, also can avoid the long period of device assembles and held up work for lack of material the time stainless steel equipment.The complexity of system reduces, thereby related engineering demand reduces too.Again need not cleaning on the throne (CIP) or sterilization on the throne (SIP) operation, and the pressure rating of relevant pipeline, valve, controller or container.In addition, the use of disposable wave bio-reactor has also reduced the complexity of checking.Because almost there is not repeatedly used assembly, just there is not any project to need to follow the tracks of yet, also just saved a large amount of sterilizations, cleaning checking research.At last, by having eliminated firm tubing system and the fixedly restriction of fermentor tank, disposable wave bio-reactor also helps realization and reequips faster for use in the operation of new technological process and the production of product innovation.In addition, the complexity of disposable wave bioreactor system and subsystem is also lower, and present disposable wave bio-reactor can reach 20-2000L aspect the scale of cultivation.
Although disposable wave bio-reactor has as above advantage and extensively utilization of acquisition in the animal cell culture production of albumen, antibody and vaccine, but then be difficult to satisfy culturing process to mixing the requirement of mass transfer and oxygen supply at oxygen consumption rate height in cell cultivation process, culturing cell density height, culturing cell particle Da Yi precipitation and the high system of culture viscosity, thereby cause culture efficiency low even cultivate and be difficult to successfully.Rely on surface ventilation and surperficial gas-liquid mixed to realize that oxygen supply must need a big specific surface area simultaneously, effective dress liquid coefficient of realizing sufficient oxygen supply reactor generally all has only 1/3 to 1/2 reactor volume, and classical pot type bio-reactor can reach more than 80%.This surperficial ventilating mode is applicable to the cell cultures of small volume bio-reactor, when use surpassing 20L even bigger bio-reactor, the surface provides the mode of gas to be difficult to satisfy the required gas supply of cell growth, particularly is difficult to satisfy the cultivation of high oxygen consumption cell.The Surface Renewal air feed causes a large amount of gas wastes simultaneously, and is especially more outstanding in technical scale.The inventive principle of the problems referred to above and disposable wave bio-reactor is inherent, therefore make present disposable wave bio-reactor cost height, use and only to be confined to expensive especially product, inapplicable cell is easy to precipitate and oxygen is transmitted and the culture system of high requirements simultaneously.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of disposable wave bio-reactor that has aerating apparatus is provided.
Another object of the present invention provides second kind of disposable wave bio-reactor that has aerating apparatus.
A kind of disposable wave bio-reactor that has aerating apparatus, comprise bag, described bag is provided with inoculation mouth, medium feed mouth, inlet mouth, air outlet, thief hole, transmitter intervention mouth and discharge port, be provided with below the described bag and make unrestrained device, the bag wall of described bag is provided with 1-40 mounting interface, the gas connection that has an inlet pipe by pipeline with pass described mounting interface and be arranged on described bag of intravital aeration head and be connected by pipeline again after sterilizing filter is connected.
Second kind of disposable wave bio-reactor that has aerating apparatus, comprise bag, described bag is provided with inoculation mouth, medium feed mouth, inlet mouth, air outlet, thief hole, transmitter intervention mouth and discharge port, be provided with below the described bag and make unrestrained device, the bag wall of described bag is provided with 1-40 mounting interface, the gas connection that has an inlet pipe by pipeline be connected with the external port of mounting interface again after sterilizing filter is connected, the inner port of mounting interface by pipeline be arranged on the intravital aeration head of described bag and be connected.
The preferred 2-15 of described mounting interface.
The bag wall of described bag comprises roof, sidewall and diapire, and described mounting interface is arranged at least one wall of described roof, sidewall and diapire.
Described aeration head is diaphragm type, bar type, turbulent flow type, tubular type, annular tube type or grid type aeration head.
The aperture of the production well of aeration head is preferably the 5-200 micron, is preferably the 50-100 micron.
The disposable wave bio-reactor that has an aerating apparatus of the present invention can provide a good air feed for the cell growth that surpasses 20L even bigger bio-reactor.Particularly to oxygen consumption rate height in cell cultivation process, culturing cell density height, and the high system of culture viscosity sufficient oxygen supply is provided, help realizing the mass-producing amplification and the industrialization of high oxygen consumption cell cultures, the gas cost can save 70%.To the less demanding cell cultures of oxygen consumption, can improve effective volume of culture of reactor simultaneously, reduce greatly and cultivate cost.The cell that has the disposable wave bioreactor culture of aerating apparatus of the present invention is better than the cell of the typical disposable wave bioreactor culture of prior art.
Description of drawings
Fig. 1 is the disposable wave bioreactor construction synoptic diagram of prior art.
Fig. 2 is the disposable wave bio-reactor that has aerating apparatus of the present invention.
Fig. 3 is the absolute growth amount of grape cell suspension culture harvested cell after 7 days in the disposable wave bio-reactor of disposable wave bio-reactor that has an aerating apparatus of the present invention and prior art.
Fig. 4 had in the disposable wave bio-reactor of the disposable wave bio-reactor of aerating apparatus and prior art suspension culture 7 days in the present invention, the degree of wherein different incubation time oxygen for the grape cell.
Fig. 5 had in the disposable wave bio-reactor of the disposable wave bio-reactor of aerating apparatus and prior art suspension culture 7 days for the grape cell in the present invention, and bio-reactor of the present invention is than the high 2-3mg/L of disposable wave bio-reactor oxygen dissolving value of prior art.
Fig. 6 is for the grape cell had in the disposable wave bio-reactor of the disposable wave bio-reactor of aerating apparatus and prior art suspension culture 7 days in the present invention, can see that having reactor of the present invention exceeds 4.4 times than the anthocyanidin of the reactor cultured cells of prior art.
Embodiment
The present invention is further illustrated below in conjunction with accompanying drawing.
A kind of disposable wave bio-reactor (see figure 2) that has aerating apparatus, comprise bag 1, bag is provided with inoculation mouth, medium feed mouth, inlet mouth, air outlet, thief hole, transmitter intervention mouth and discharge port, be provided with below the bag and make unrestrained device 7, the bag wall of bag is provided with 1-40 mounting interface 2, the gas connection 6 that has an inlet pipe 3 by pipeline with pass described mounting interface and be arranged on a bag intravital aeration head 4 and be connected by pipeline again after sterilizing filter 5 is connected.
Second kind of disposable wave bio-reactor that has aerating apparatus, comprise bag, described bag is provided with inoculation mouth, medium feed mouth, inlet mouth, air outlet, thief hole, transmitter intervention mouth and discharge port, be provided with below the described bag and make unrestrained device, the bag wall of described bag is provided with 1-40 mounting interface, the gas connection that has an inlet pipe by pipeline be connected with the external port of mounting interface again after sterilizing filter is connected, the inner port of mounting interface by pipeline be arranged on the intravital aeration head of described bag and be connected.
Preferred 2,3,4 of mounting interfaces ... 15.
The bag wall of bag comprises roof, sidewall and diapire, mounting interface can be arranged on the roof, also can be arranged on the sidewall, also can be arranged on the diapire, also can be arranged on roof and the sidewall as required simultaneously or be arranged on roof and the diapire simultaneously or be arranged on simultaneously on sidewall and the diapire, also can send out and be arranged on simultaneously on roof, sidewall and the diapire.
According to the demand of cell cultures, select multi-form aeration head for use, can select diaphragm type, bar type, turbulent flow type, tubular type, annular tube type or grid type aeration head.
The aperture of the production well of aeration head is preferably the 5-200 micron, is preferably the 50-100 micron.
The present invention introduces the ventilation under the liquid simultaneously in the disposable wave bio-reactor that only uses the surface ventilation, at the cell cultures of high oxygen consumption demand to solve the insufficient problem of its oxygen supply.Inlet pipe on the gas connection can be a plurality of, can be connected with multiple source of the gas, feeds single or blended gaseous constituent according to the cultivation demand.According to cell cultures to the demand of gaseous constituent can the adjustments of gas moiety, aeration head form, the distribution of aeration head in nutrient solution.
The present invention is further illustrated below by specific embodiment.
The grape cell cultures
The disposable wave bio-reactor model of prior art is 20L, the disposable wave bio-reactor that has aerating apparatus of the present invention also is 20L, but the volume of culture difference of cell, the disposable wave bioreactor culture cell 30L that has aerating apparatus of the present invention, the disposable wave bioreactor culture cell 20L of prior art, every index of grape cell is all qualified, but it is higher by 50% than the cell absolute growth amount of the normal disposable wave bio-reactor of prior art to have the cell absolute growth amount of disposable wave bio-reactor of aerating apparatus.See Fig. 3, shown in the figure for the absolute growth amount of grape cell suspension culture harvested cell after 7 days in the disposable wave bio-reactor of disposable wave bio-reactor that has aerating apparatus and prior art.
What present embodiment adopted is first kind of disposable wave bio-reactor that has aerating apparatus, and through experiment, second kind of disposable wave bio-reactor that has an aerating apparatus is used for cell cultures and also has similar effect.
The grape cell cultures
The grape cell had in the disposable wave bio-reactor of the disposable wave bio-reactor of aerating apparatus and 200L prior art suspension culture 7 days at 200L of the present invention, the degree of wherein different incubation time oxygen, can obviously draw the disposable wave bio-reactor that 200L of the present invention has aerating apparatus and will save 60%, see Fig. 4 than the disposable wave bio-reactor of the 200L of prior art oxygen consuming amount.
What present embodiment adopted is first kind of disposable wave bio-reactor that has aerating apparatus, and through experiment, second kind of disposable wave bio-reactor that has an aerating apparatus is used for cell cultures and also has similar effect.
Embodiment 3
The grape cell cultures
The grape cell had in the disposable wave bio-reactor of the disposable wave bio-reactor of aerating apparatus and 200L prior art suspension culture 7 days at 200L of the present invention, can obviously find out the reactor oxygen dissolving value high 2-3mg/L of the disposable wave bio-reactor of aerating apparatus of the present invention than prior art.Illustrate that effect of the present invention is obvious, advantage is outstanding, sees Fig. 5.
What present embodiment adopted is first kind of disposable wave bio-reactor that has aerating apparatus, and through experiment, second kind of disposable wave bio-reactor that has an aerating apparatus is used for cell cultures and also has similar effect.
The grape cell had in the disposable wave bio-reactor of aerating apparatus and the disposable wave bio-reactor of 200L suspension culture 7 days at 200L of the present invention, and the disposable wave bio-reactor that can see aerating apparatus of the present invention exceeds 4.4 times than the anthocyanidin of the reactor cultured cells of prior art.Solve industrial cell volume and amplified the difficult problem that back cell itself does not produce target product, seen Fig. 6.
What present embodiment adopted is first kind of disposable wave bio-reactor that has aerating apparatus, and through experiment, second kind of disposable wave bio-reactor that has an aerating apparatus is used for cell cultures and also has similar effect.
The present invention is to be example to cultivate the grape cell, is for effect of the present invention is described, but is not limited to the material of being cultivated.
Claims (7)
1. disposable wave bio-reactor that has aerating apparatus, comprise bag, described bag is provided with inoculation mouth, medium feed mouth, inlet mouth, air outlet, thief hole, transmitter intervention mouth and discharge port, be provided with below the described bag and make unrestrained device, a bag wall that it is characterized in that described bag is provided with 1-40 mounting interface, the gas connection that has an inlet pipe by pipeline with pass described mounting interface and be arranged on described bag of intravital aeration head and be connected by pipeline again after sterilizing filter is connected.
2. disposable wave bio-reactor that has aerating apparatus, comprise bag, described bag is provided with inoculation mouth, medium feed mouth, inlet mouth, air outlet, thief hole, transmitter intervention mouth and discharge port, be provided with below the described bag and make unrestrained device, the bag wall that it is characterized in that described bag is provided with 1-40 mounting interface, the gas connection that has an inlet pipe by pipeline be connected with the external port of mounting interface again after sterilizing filter is connected, the inner port of mounting interface by pipeline be arranged on the intravital aeration head of described bag and be connected.
3. a kind of disposable wave bio-reactor that has aerating apparatus according to claim 1 and 2 is characterized in that described mounting interface is 2-15.
4. a kind of disposable wave bio-reactor that has aerating apparatus according to claim 1 and 2, the bag wall that it is characterized in that described bag comprises roof, sidewall and diapire, and described mounting interface is arranged at least one wall of described roof, sidewall and diapire.
5. a kind of disposable wave bio-reactor that has aerating apparatus according to claim 1 and 2 is characterized in that described aeration head is diaphragm type, bar type, turbulent flow type, tubular type, annular tube type or grid type aeration head.
6. a kind of disposable wave bio-reactor that has aerating apparatus according to claim 1 and 2, the aperture that it is characterized in that the production well of aeration head is the 5-200 micron.
7. a kind of disposable wave bio-reactor that has aerating apparatus according to claim 6, the aperture that it is characterized in that the production well of aeration head is the 50-100 micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210021722.6A CN103224882B (en) | 2012-01-31 | 2012-01-31 | Disposable wave type bioreactor with aerating apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210021722.6A CN103224882B (en) | 2012-01-31 | 2012-01-31 | Disposable wave type bioreactor with aerating apparatus |
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| CN103224882A true CN103224882A (en) | 2013-07-31 |
| CN103224882B CN103224882B (en) | 2014-07-30 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805013A (en) * | 2015-05-25 | 2015-07-29 | 固元本草汉方生物科技股份有限公司 | Reactor for disposable airlift type plant root tissue culture |
| CN104823858A (en) * | 2015-05-25 | 2015-08-12 | 固元本草汉方生物科技股份有限公司 | Disposable air-lifting type plant seed tissue culture reactor |
| CN105368888A (en) * | 2015-11-27 | 2016-03-02 | 天津艾赛博生物技术有限公司 | Method of producing taxane through large-scale culture by Taxus cell line three-line technology |
| CN106754633A (en) * | 2017-02-14 | 2017-05-31 | 天津艾赛博生物技术有限公司 | A kind of regulation and control method of pilot scale culture Herba Saussureae Involueratae cell high yield flavones |
| CN106867958A (en) * | 2017-02-14 | 2017-06-20 | 天津艾赛博生物技术有限公司 | A kind of technique of extensive squamous subculture Herba Saussureae Involueratae cell |
| CN111989391A (en) * | 2018-04-25 | 2020-11-24 | 环球生命科技咨询美国有限责任公司 | Inflatable bioreactor and method of use |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030036192A1 (en) * | 2001-02-15 | 2003-02-20 | Vijay Singh | Disposable perfusion bioreactor for cell culture |
| CN101812405A (en) * | 2010-04-30 | 2010-08-25 | 惠倪 | Polygonal plastic bag swing cell reactor and method for culturing animal cells |
| CN102021115A (en) * | 2009-09-21 | 2011-04-20 | 惠识瑶 | Packed bed type cell bioreactor without stirrer and method for cultivating animal cells |
-
2012
- 2012-01-31 CN CN201210021722.6A patent/CN103224882B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030036192A1 (en) * | 2001-02-15 | 2003-02-20 | Vijay Singh | Disposable perfusion bioreactor for cell culture |
| CN102021115A (en) * | 2009-09-21 | 2011-04-20 | 惠识瑶 | Packed bed type cell bioreactor without stirrer and method for cultivating animal cells |
| CN101812405A (en) * | 2010-04-30 | 2010-08-25 | 惠倪 | Polygonal plastic bag swing cell reactor and method for culturing animal cells |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805013A (en) * | 2015-05-25 | 2015-07-29 | 固元本草汉方生物科技股份有限公司 | Reactor for disposable airlift type plant root tissue culture |
| CN104823858A (en) * | 2015-05-25 | 2015-08-12 | 固元本草汉方生物科技股份有限公司 | Disposable air-lifting type plant seed tissue culture reactor |
| CN105368888A (en) * | 2015-11-27 | 2016-03-02 | 天津艾赛博生物技术有限公司 | Method of producing taxane through large-scale culture by Taxus cell line three-line technology |
| CN106754633A (en) * | 2017-02-14 | 2017-05-31 | 天津艾赛博生物技术有限公司 | A kind of regulation and control method of pilot scale culture Herba Saussureae Involueratae cell high yield flavones |
| CN106867958A (en) * | 2017-02-14 | 2017-06-20 | 天津艾赛博生物技术有限公司 | A kind of technique of extensive squamous subculture Herba Saussureae Involueratae cell |
| CN111989391A (en) * | 2018-04-25 | 2020-11-24 | 环球生命科技咨询美国有限责任公司 | Inflatable bioreactor and method of use |
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