CN203855567U - Visual strain culture expanding fermentation tank device - Google Patents
Visual strain culture expanding fermentation tank device Download PDFInfo
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- CN203855567U CN203855567U CN201420164767.3U CN201420164767U CN203855567U CN 203855567 U CN203855567 U CN 203855567U CN 201420164767 U CN201420164767 U CN 201420164767U CN 203855567 U CN203855567 U CN 203855567U
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- blind flange
- sampling valve
- filtration system
- valve
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Abstract
The utility model relates to the technical field of strain culture expanding equipment. According to the technical scheme, the visual strain culture expanding fermentation tank device comprises a fermentation tank, an aeration rod mask, an aeration rod, a long sight glass, a handle, a blind flange, a sealed spanner, an inoculation opening, a sterile sampling valve port, a sterile sampling valve, an air filter, a filter element, an air vent, a sterile filtering system A, a sterile filtering system B, a flow meter, a valve, an oxygen bottle or an air compressor. The culture solution is added into the fermentation tank and is heated and sterilized; after the temperature of the culture solution is reduced, oxygen is introduced, the solution is observed through the long sight glass, and the aeration amount is regulated through the flow meter; after aeration is ended, the strain is added into the fermentation tank in a sterile environment, and fermentation is performed; after fermentation is ended, air is introduced into the fermentation tank through the air vent, the fermentation liquor is pressed from the sterile sampling valve port to the an inoculation container; visualization of the whole fermentation process is realized; and moreover, the dissolved oxygen in the culture solution is increased, the accuracy of the oxygenation amount in the fermentation process is improved, and the strain culture expanding stability is improved.
Description
Technical field
The utility model relates to the bacterial classification equipment technical field that spreads cultivation, and is specifically related to a kind of visual bacterial classification fermentor device that spreads cultivation.
Background technology
At present, in laboratory or the bacterial classification that uses of the teaching fermentor tank that spreads cultivation, can carry out sterilization and cultivate purebred bacterial classification meeting the needs that bacterial classification spreads cultivation to nutrient solution, but exist, cannot examine fermenting process and the problem such as fermenting process oxygenation capacity is inaccurate, be unfavorable for the spread cultivation control of process of bacterial classification.
The utility model can solve the visual of fermentation whole process, has increased the acquaintance to fermenting process, improves the visual effect of teaching or experiment; During oxygenation, increase the contact area of oxygen and nutrient solution, increased the dissolved oxygen amount of nutrient solution, promoted the growth and breeding of bacterial classification; Be furnished with oxygen flow meter, can carry out record to oxygenation capacity, be convenient to the statistics and analysis of data, find out best oxygenation capacity, improved the accuracy of fermenting process oxygenation capacity, improved the stability that bacterial classification spreads cultivation.
Summary of the invention
The utility model is for the fermentor device that overcomes that existing bacterial classification spreads cultivation fermentor tank and a kind of visual bacterial classification that designs spreads cultivation.
The utility model solves the technical scheme that its technical problem adopts: the visual bacterial classification fermentor device that spreads cultivation, it comprises fermentor tank (1), logical oxygen bar mouth mask (2), logical oxygen bar (3), long visor (4), handle (5), blind flange (6), sealing spanner (7), inoculation mouthful (8), sterile sampling valve port (9), sterile sampling valve valve (10), air filter (11), filter core (12), ventage (13), sterile filtration system A (14), sterile filtration system B (15), under meter (16), valve (17), oxygen cylinder or air compressor machine (18), it is characterized in that, described fermentor tank (1) has blind flange (6) and handle (5) above, blind flange (6) comprises, lower flange and sealing-ring, by a plurality of sealing spanners (7) that are evenly distributed by blind flange (6) and airtight connection of fermentor tank (1), handle (5) can facilitate mobile tank body, on described blind flange (6), there are three openings, have respectively inoculation mouth, sterile sampling valve and air filter (11), inoculation has sealing cover on mouthful (8), and inoculation mouthful is connected with round thread with sealing cover, has food grade silica gel sealing pad in sealing cover, by inoculation mouthful (8), can inject bacterial classification to fermentor tank, the above part of blind flange of sterile sampling valve has sterile sampling valve mouth (9) and sterile sampling valve valve (10), the following part of blind flange is connected with logical oxygen bar (3) and logical oxygen bar mouth mask (2), logical oxygen bar mouth mask (2) is from 0.5cm place at the bottom of tank, logical oxygen bar mouth mask (2) is connected with round thread with logical oxygen bar (3), for convenience detach and cleaning, the mesh that has even size on logical oxygen bar mouth mask (2), the oxygen of being convenient to be filled with forms tiny bubble, can increase the contact area of oxygen and nutrient solution, increase the dissolved oxygen amount of nutrient solution, promote the growth and breeding of bacterial classification, air filter (11) lower end has mouth to be connected with blind flange (6), and communicate with fermentor tank, air filter (11) is connected with round thread slip-knot with blind flange (6), in air filter (11), there is filter core (12), filtration for air or oxygen, ventage (13) is arranged at top, for passing into sterile air or oxygen to fermentor tank (1), also can be used for gas in tank and discharges, described long visor (4) is positioned on fermentor tank (1) tank body, by the transparent high pressure resistant material of food grade, made, will long visor (4) and airtight connection of fermentor tank (1) by nut, screw, food grade silicagel pad etc., can observe the whole process of fermenting in fermentor tank (1) by this long visor (4), described sterile filtration system A (14) and sterile filtration system B (15), inside have filtration medium, can filter air or oxygen, forms sterile air or clean oxygen, between sterile filtration system A (14) and sterile filtration system B (15), between sterile filtration system A (14) and sterile sampling valve port (9), connection between sterile filtration system B (15) and oxygen cylinder or air compressor machine (18) outlet pipe is hose connection by food grade, under meter (16) and valve (17) are installed on oxygen cylinder or air compressor machine outlet pipe, under meter (16) can show uninterrupted, the size of valve (17) controllable flow rate, can carry out the statistics and analysis of oxygenation data, be convenient to explore the best oxygenation capacity of fermentation, improve the accuracy of fermenting process oxygenation capacity, improve the stability that bacterial classification spreads cultivation.
The beneficial effects of the utility model are: owing to having adopted the visual bacterial classification fermentor device that spreads cultivation, is furnished with long visor (4), observe the whole process of fermentation in fermentor tank (1), realized the visual of fermentation whole process, increase the acquaintance of fermenting process, improved the visual effect of teaching or experiment; Be furnished with sterile filtration system A (14) and sterile filtration system B (15), can filtrated air or oxygen in bacterium and impurity, be conducive to carrying out smoothly of fermenting process; Be furnished with logical oxygen bar mouth mask (2), have the mesh of even size on it, the oxygen of being convenient to be filled with forms tiny bubble, can increase the contact area of oxygen and nutrient solution, increases the dissolved oxygen amount of nutrient solution, promotes the growth and breeding of bacterial classification; Be furnished with under meter (16) and valve (17), under meter (16) can show uninterrupted, the size of valve (17) controllable flow rate, can carry out the statistics and analysis of oxygenation data, be convenient to explore the best oxygenation capacity of fermentation, improve the accuracy of fermenting process oxygenation capacity, improve the stability that bacterial classification spreads cultivation.
accompanying drawing explanation:
Figure is the spread cultivation structural representation of fermentor device of visual bacterial classification.
In figure: 1. fermentor tank, 2. logical oxygen bar mouth mask, 3. logical oxygen bar, 4. long visor, 5. handle, 6. blind flange, 7. sealing spanner, 8. inoculates mouth, 9. sterile sampling valve port, 10. sterile sampling valve valve, 11. air filters, 12. filter cores, 13. ventages, 14. sterile filtration system A, 15. sterile filtration system B, 16. under meters, 17. valves, 18. oxygen cylinders or air compressor machine.
Embodiment
With reference to accompanying drawing, the visual bacterial classification fermentor device that spreads cultivation is done to following explanation.The visual bacterial classification fermentor device that spreads cultivation, it comprises fermentor tank (1), logical oxygen bar mouth mask (2), logical oxygen bar (3), long visor (4), handle (5), blind flange (6), sealing spanner (7), inoculation mouth (8), a sterile sampling valve port (9), sterile sampling valve valve (10), air filter (11), filter core (12), ventage (13), sterile filtration system A (14), sterile filtration system B (15), under meter (16), valve (17), oxygen cylinder or air compressor machine (18), before bacterial classification spreads cultivation, the blind flange on fermentor tank (1) is opened, fermentor tank (1) inside is cleaned up with tap water, then each element purge such as inoculation mouthful (8), sterile sampling valve port (9), sterile sampling valve valve (10), logical oxygen bar (3), logical oxygen bar mouth mask (2), air filter (11), filter core (12) is clean, these parts are installed and be fastened on blind flange, to fermentor tank (1), add the nutrient solution that accounts for total volume 80%, build blind flange (6), the sealing cover of inoculation mouthful (8) of outwarding winding, bandages ventage (13) with cotton, prevents ventage contact external environment, do a good job of it handle (5), fermentor tank (1) is positioned in high pressure steam pot or gas furnace, electromagnetic oven on micro-sterilization 30 minutes of boiling, simultaneously also by sterile filtration system A (14), sterile filtration system B (15) High Temperature Sterilization 30 minutes, the sealing cover of ventage in heat-processed (13) and inoculation mouthful (8) is all sterilized by hot steam, after High Temperature Sterilization, screw the sealing cover of inoculation mouthful (8), fermentor tank is placed in cold house, makes fermentation jar temperature be down to room temperature, with the food grade flexible pipe disinfecting, successively the outlet pipe of sterile sampling valve port (9), sterile filtration system A (14), sterile filtration system B (15), oxygen cylinder or air compressor machine (18) is connected, open sterile sampling valve valve (10), Open valve (17), lead to oxygen or blowing air, the oxygen passing into or air are discharged by ventage (13), by long visor (4), observe the Air Bubble Size that logical oxygen bar mouth mask (2) comes up, regulated valve (17) size, controls under meter (16) to certain data, and steadily logical oxygen, records logical oxygen flow and time, after logical oxygen finishes, valve-off (17), closes sterile sampling valve valve (10), takes off the upper flexible pipe being connected with the outlet pipe of oxygen cylinder or air compressor machine (18) of sterile filtration system B (15), in gnotobasis, the sealing cover of inoculating mouthful (8) is taken off, bacterial classification is directly added to fermentor tank (1), at the suitable temperature of bacterial classification, the fermentation culture regular hour, after fermentation ends, under gnotobasis, remove the connecting hose between sterile sampling valve port (9) and sterile filtration system A (14), with the food grade flexible pipe disinfecting, the pipe joint of sterile sampling valve port (9) and inoculation container (being generally larger fermentor tank) is connected, take off the cotton of the upper wrapping of ventage (13), ventage (13) and the outlet pipe of oxygen cylinder or air compressor machine (18) are connected, open sterile sampling valve valve (10), valve (17) and inoculation container valve, the clean oxygen of filtering through air filter (11) or sterile air are passed into fermentor tank (1) fermented liquid top, strain fermentating liquid is forced into inoculation container from sterile sampling valve port (9), air or oxygen is supplied with pressure must not surpass 0.03MPa, from long visor (4), observe, after fermented liquid has been transferred, the valve of valve-off (17), sterile sampling valve valve (10) and inoculation container, pulls down each connecting hose successively, and strain fermentating liquid has shifted out.
Finish using, open sealing spanner (7), blind flange (6) is opened, inside and outside cleaning fermentor tank, to inoculate mouthful (8), logical oxygen bar (3), logical oxygen bar mouth mask (2), sterile sampling valve port (9) etc. and clean, after filtering element for air filter (12) takes out, clean up and alcohol disinfecting, install and recover former state, in order to using next time.
Above-mentioned explanation is not to restriction of the present utility model; the utility model is also not limited in above-mentioned giving an example; the variation that those skilled in the art make in essential scope of the present utility model, remodeling, interpolation or replacement, also should belong to protection domain of the present utility model.
Claims (1)
1. the visual bacterial classification fermentor device that spreads cultivation, it comprises fermentor tank (1), logical oxygen bar mouth mask (2), logical oxygen bar (3), long visor (4), handle (5), blind flange (6), sealing spanner (7), inoculation mouthful (8), sterile sampling valve port (9), sterile sampling valve valve (10), air filter (11), filter core (12), ventage (13), sterile filtration system A (14), sterile filtration system B (15), under meter (16), valve (17), oxygen cylinder or air compressor machine (18), it is characterized in that, described fermentor tank (1) has blind flange (6) and handle (5) above, blind flange (6) comprises, lower flange and sealing-ring, by a plurality of sealing spanners (7) that are evenly distributed by blind flange (6) and airtight connection of fermentor tank (1), handle (5) can facilitate mobile tank body, on described blind flange (6), there are three openings, have respectively inoculation mouth, sterile sampling valve and air filter (11), inoculation has sealing cover on mouthful (8), and inoculation mouthful is connected with round thread with sealing cover, has food grade silica gel sealing pad in sealing cover, the above part of blind flange of sterile sampling valve has sterile sampling valve mouth (9) and sterile sampling valve valve (10), the following part of blind flange is connected with logical oxygen bar (3) and logical oxygen bar mouth mask (2), logical oxygen bar mouth mask (2) is connected with round thread with logical oxygen bar (3), has the mesh of even size on logical oxygen bar mouth mask (2), air filter (11) lower end has mouth to be connected with blind flange (6), and communicates with fermentor tank, and air filter (11) is connected with round thread slip-knot with blind flange (6), has filter core (12) in air filter (11), and ventage (13) is arranged at top, described long visor (4) is positioned on fermentor tank (1) tank body, by the transparent high pressure resistant material of food grade, is made, will long visor (4) and airtight connection of fermentor tank (1) by nut, screw, food grade silicagel pad etc., between described sterile filtration system A (14) and sterile filtration system B (15), connection between sterile filtration system A (14) and sterile sampling valve port (9), between sterile filtration system B (15) and oxygen cylinder or air compressor machine (18) outlet pipe is hose connection by food grade, and under meter (16) and valve (17) are installed on oxygen cylinder or air compressor machine outlet pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420164767.3U CN203855567U (en) | 2014-04-08 | 2014-04-08 | Visual strain culture expanding fermentation tank device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420164767.3U CN203855567U (en) | 2014-04-08 | 2014-04-08 | Visual strain culture expanding fermentation tank device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203855567U true CN203855567U (en) | 2014-10-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420164767.3U Expired - Fee Related CN203855567U (en) | 2014-04-08 | 2014-04-08 | Visual strain culture expanding fermentation tank device |
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| CN (1) | CN203855567U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611192A (en) * | 2015-02-02 | 2015-05-13 | 江苏星海生物科技有限公司 | Novel microorganism fermentation tank |
| CN114606118A (en) * | 2022-04-18 | 2022-06-10 | 智享生物科技(苏州)有限公司 | Bioreactor for uniformly oxygenating culture solution for biological cultivation |
| CN117568164A (en) * | 2023-11-16 | 2024-02-20 | 广东青云山药业有限公司 | Preparation equipment and method of sugar-free hawthorn extract with lipid-lowering effect |
-
2014
- 2014-04-08 CN CN201420164767.3U patent/CN203855567U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611192A (en) * | 2015-02-02 | 2015-05-13 | 江苏星海生物科技有限公司 | Novel microorganism fermentation tank |
| CN114606118A (en) * | 2022-04-18 | 2022-06-10 | 智享生物科技(苏州)有限公司 | Bioreactor for uniformly oxygenating culture solution for biological cultivation |
| CN117568164A (en) * | 2023-11-16 | 2024-02-20 | 广东青云山药业有限公司 | Preparation equipment and method of sugar-free hawthorn extract with lipid-lowering effect |
| CN117568164B (en) * | 2023-11-16 | 2024-05-24 | 广东青云山药业有限公司 | Preparation equipment and method of sugar-free hawthorn extract with lipid-lowering effect |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141001 Termination date: 20170408 |
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| CF01 | Termination of patent right due to non-payment of annual fee |