CN105494096A - Novel plant tissue culture flask - Google Patents

Novel plant tissue culture flask Download PDF

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Publication number
CN105494096A
CN105494096A CN201510942100.0A CN201510942100A CN105494096A CN 105494096 A CN105494096 A CN 105494096A CN 201510942100 A CN201510942100 A CN 201510942100A CN 105494096 A CN105494096 A CN 105494096A
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bottle
cylinder
tissue culture
plant tissue
novel plant
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徐德林
沈访
钱刚
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Zunyi Medical University
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Zunyi Medical University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本专利公开了一种新型的植物组织培养瓶,包括瓶罩和瓶缸,所述瓶罩密封可拆卸连接在瓶缸上方,所述瓶罩包括圆柱形瓶身以及与瓶身一体成型的弧顶,所述弧顶的中部设有进气管,瓶身上设有出气管;瓶缸的高度为瓶身高度的1/3~1/2,所述瓶缸底部连接有瓶托;瓶身设有内螺纹段,瓶缸上设有与内螺纹段啮合的外螺纹段;进气管和出气管上均设有可拆卸的微孔滤菌器;进气管和出气管的管口上设有通过柔性件连接的圆台形闭合盖。本发明设计的组合分体闭合式培养瓶,瓶罩与瓶缸为分体结构,瓶缸的大小即为管径大小,需要操作、实验记录拍照以及炼苗移栽时,将瓶罩与瓶缸分离即可,具有便于直视下操作、不宜损伤植物的根、茎、叶的优点。

This patent discloses a new type of plant tissue culture bottle, which includes a bottle cover and a bottle cylinder. The bottle cover is sealed and detachably connected above the bottle cylinder. The bottle cover includes a cylindrical bottle body and an arc integrally formed with the bottle body. top, the middle part of the arc top is provided with an air inlet pipe, and the bottle body is provided with an air outlet pipe; the height of the bottle cylinder is 1/3~1/2 of the height of the bottle body, and the bottom of the bottle cylinder is connected with a bottle holder; There is an internal thread section, and an external thread section that engages with the internal thread section is provided on the cylinder; detachable microporous bacteria filters are provided on both the inlet pipe and the outlet pipe; the nozzles of the inlet pipe and the outlet pipe are equipped with flexible The frustum-shaped closing cover connected by pieces. In the combined split closed culture bottle designed by the present invention, the bottle cover and the bottle cylinder are of a split structure, and the size of the bottle cylinder is the size of the pipe diameter. The cylinder can be separated, which has the advantages of being easy to operate under direct vision and not damaging the roots, stems and leaves of plants.

Description

一种新型的植物组织培养瓶A new type of plant tissue culture bottle

技术领域 technical field

本发明涉及一种组织培养的植物再生技术领域,具体涉及一种新型的植物组织培养瓶。 The invention relates to the technical field of tissue culture plant regeneration, in particular to a novel plant tissue culture bottle.

背景技术 Background technique

1902年德国植物学家哈伯兰特提出“植物细胞全能性”理论,1958年美国植物学家斯蒂瓦特等人用胡萝卜韧皮部的细胞进行培养,获得了能够开花结果的完整植株,证实了“细胞全能性”这一预言,从而造就了一项新技术——植物组织培养技术,广义上又叫“立体培养”。植物组织培养技术不仅是无性繁殖和种苗快速繁殖的重要手段,也是细胞杂交、基因改良等技术体系的重要组成部分。通过建立一个适宜的快速组织培养体系,不仅解决了其野生资源日渐枯竭的现实问题,也能为细胞杂交、转基因等生物技术遗传改良打下坚实基础。以兰科植物为例,正如Arditti和Emes在《MicropropagationofOrchids》一书中所说的那样,“复印机和兰科组培技术的同时问世,使得世界从此不再一成不变”。 In 1902, German botanist Haberlandt proposed the theory of "plant cell totipotency". In 1958, American botanist Stewart and others used carrot phloem cells to cultivate and obtained a complete plant capable of flowering and fruiting, confirming the "totipotency of plant cells". The prediction of "cell totipotency" has created a new technology - plant tissue culture technology, which is also called "three-dimensional culture" in a broad sense. Plant tissue culture technology is not only an important means for asexual reproduction and rapid seedling propagation, but also an important part of technical systems such as cell hybridization and genetic improvement. By establishing a suitable rapid tissue culture system, it not only solves the practical problem of the depletion of its wild resources, but also lays a solid foundation for the genetic improvement of biotechnology such as cell hybridization and transgenic. Take orchids as an example, as Arditti and Emes said in the book "Micropropagation of Orchids", "The simultaneous advent of copiers and orchid tissue culture technology has made the world no longer the same."

植物受自身生物学特性、生态环境等因素的限制,野生资源已日渐枯竭,尤其是药用植物资源,自然条件下发芽率极低,生育周期相对较长,在一定程度上增加了生产成本而降低了经济效益。如何在较短时间内获得大量种苗,缩短培养周期,降低成本,成为生产过程中急需解决的问题。因此,通过组织培养技术,提高种子发芽率,人工快速繁殖种苗是保护植物资源和解决产业化对种苗需求的有效途径。 Plants are limited by their own biological characteristics, ecological environment and other factors, and wild resources have been depleted day by day, especially medicinal plant resources. Under natural conditions, the germination rate is extremely low and the growth cycle is relatively long, which increases production costs to a certain extent. Reduced economic benefits. How to obtain a large number of seedlings in a short period of time, shorten the cultivation period, and reduce costs have become urgent problems in the production process. Therefore, it is an effective way to protect plant resources and meet the needs of industrialization for seedlings by improving the germination rate of seeds through tissue culture technology and artificial rapid propagation of seedlings.

植物组织培养瓶为组织培养营造了一个无菌的环境,当前使用的组织瓶主要由瓶身、瓶盖或瓶塞组成,一方面,瓶身为一体成型,当加入培养基后培养基与瓶口距离较远,不利于操作,尤其是根系发达的植物;瓶内外空气流通差,当内外温差大时就造成瓶壁积水或水雾,不利于实验拍照记录,甚至增加污染率,另一方面,细小的瓶口在组培苗经炼苗移栽取苗时易损伤植物的根、茎、叶,进而影响植株的存活率。 Plant tissue culture bottles create a sterile environment for tissue culture. Currently used tissue bottles are mainly composed of bottle body, bottle cap or bottle stopper. On the one hand, the bottle body is integrally formed. The mouth is far away, which is not conducive to operation, especially for plants with well-developed root systems; the air circulation inside and outside the bottle is poor, and when the temperature difference between inside and outside is large, it will cause water or mist on the wall of the bottle, which is not conducive to taking photos and recording experiments, and even increases the pollution rate. On the one hand, the small bottle mouth is easy to damage the roots, stems and leaves of the plants when the tissue culture seedlings are transplanted and taken out, which in turn affects the survival rate of the plants.

发明内容 Contents of the invention

本发明意在提供一种新型的培养瓶,以解决一体成型的瓶身带来操作不变、实验记录不清晰以及移栽时容易损伤植物的缺陷。 The present invention intends to provide a new type of culture bottle to solve the defects of unchanging operation, unclear experimental records and easy damage to plants during transplanting due to the integrally formed bottle body.

本方案中的一种新型的植物组织培养瓶,包括相互套接可拆卸的瓶罩和瓶缸,所述瓶罩包括圆柱形瓶身以及与瓶身一体成型的弧顶,所述弧顶的中部设有进气管,瓶身上且与弧顶的结合处的下部设有出气管;瓶缸的高度为瓶身高度的1/3~1/2,所述瓶身与瓶缸相对密封,所述瓶缸底部连接有瓶托。 A new type of plant tissue culture bottle in this solution includes a bottle cover and a bottle cylinder which are mutually socketed and detachable. The bottle cover includes a cylindrical bottle body and an arc top integrally formed with the bottle body. There is an air inlet pipe in the middle, and an air outlet pipe on the lower part of the bottle body and at the junction with the arc top; the height of the bottle cylinder is 1/3~1/2 of the height of the bottle body, and the bottle body and the bottle cylinder are relatively sealed, so The bottom of the bottle jar is connected with a bottle holder.

本发明的工作原理及有益效果:本发明通过对兰科植物石斛、白及的组织培养总结,设计了一种组合分体闭合式培养瓶,瓶罩密封可拆卸连接在瓶缸上方,将瓶罩与瓶缸形成分体结构,瓶缸的大小即为管径大小,宽大的管径尤其适用于根系发达的植物,需要操作、实验记录拍照以及炼苗移栽时,将瓶罩与瓶缸分离即可。具有便于直视下操作、不宜损伤植物的根、茎、叶的优点,当瓶身与瓶缸套接闭合时,瓶罩与瓶缸形成植物生长相对的密封空间,瓶罩上的进气管和出气管通过与外界空气流通,能有效地解决培养瓶内外温差造成的瓶壁积水或水雾;同时通过瓶壁上的进气管向瓶内通入气体增加植物光合作用,有效解决当前植物组培瓶的缺陷,为植物组织培养提供器具保障,且具有非常广阔的市场前景。 Working principle of the present invention and beneficial effect: the present invention has designed a kind of combination split closed type culture bottle by summarizing the tissue culture of Orchidaceae dendrobium, white and, the bottle cover seal is detachably connected on the top of the bottle vat, the bottle The cover and the vat form a split structure. The size of the vat is the size of the pipe diameter. The wide pipe diameter is especially suitable for plants with well-developed root systems. When it is necessary to operate, take photos of experimental records, and transplant seedlings, the bottle cover and vat Just separate. It has the advantages of being easy to operate under direct vision and not harmful to the roots, stems and leaves of plants. When the bottle body and the bottle cylinder are sleeved and closed, the bottle cover and the bottle cylinder form a sealed space where the plants grow opposite. The air intake pipe on the bottle cover and the The outlet pipe communicates with the outside air, which can effectively solve the water accumulation or water mist on the bottle wall caused by the temperature difference between the inside and outside of the culture bottle; at the same time, the gas is introduced into the bottle through the air inlet pipe on the bottle wall to increase the photosynthesis of plants, effectively solving the problem of current plant composition. The defect of the culture bottle provides equipment guarantee for plant tissue culture, and has a very broad market prospect.

进一步,所述瓶身设有内螺纹段,瓶缸上设有与内螺纹段啮合的外螺纹段,内螺纹段的上部设有耐高温的密封圈。瓶罩与瓶缸通过螺纹啮合连接,具有安装和拆卸容易的特点,且内螺纹段上部设有的密封圈时培养瓶的密封效果更好。 Further, the bottle body is provided with an internal thread section, the bottle cylinder is provided with an external thread section engaging with the internal thread section, and a high temperature resistant sealing ring is provided on the upper part of the internal thread section. The bottle cover and the bottle cylinder are connected by thread engagement, which has the characteristics of easy installation and disassembly, and the sealing effect of the culture bottle is better when the sealing ring is provided on the upper part of the internal thread section.

进一步,所述进气管和出气管上均设有可拆卸的微孔滤菌器(微孔滤菌器为微孔滤膜过滤器,主要由不锈钢过滤系统、真空系统、机箱和电器等部分组成,结构合理、外形美观、表面光洁、易清洁、耐腐蚀、使用方便、经久耐用;是大专院校、科研单位、药厂、医院等单位作为培养基、血液制品、药液等过滤细菌、微生物和微粒的设备),所述微孔滤菌器中微孔直径为0.22um。植物组织培养需要在无菌的环境中进行,微孔直径为0.22um的微孔滤菌器的滤菌效果更好。 Further, the air inlet pipe and the air outlet pipe are provided with detachable microporous bacteria filters (the microporous bacteria filters are microporous membrane filters, mainly composed of stainless steel filter systems, vacuum systems, chassis and electrical appliances, etc. , reasonable structure, beautiful appearance, smooth surface, easy to clean, corrosion resistance, easy to use, durable; it is used by colleges, scientific research institutes, pharmaceutical factories, hospitals and other units as culture medium, blood products, liquid medicine, etc. to filter bacteria and microorganisms and particle equipment), the diameter of the micropores in the microporous bacteria filter is 0.22um. Plant tissue culture needs to be carried out in a sterile environment, and the microporous bacteria filter with a micropore diameter of 0.22um has a better bacterial filtration effect.

进一步,所述进气管和出气管的管口上设有通过柔性件连接的圆台形闭合盖。无须向培养瓶输入空气时,将微孔滤菌器取下,使用闭合盖将管口堵住,圆台形的闭合盖使密封效果更好。 Further, the nozzles of the air inlet pipe and the air outlet pipe are provided with a frustum-shaped closing cover connected by a flexible piece. When there is no need to input air into the culture bottle, the microporous bacteria filter is removed, and the nozzle is blocked with a closing cap. The frustum-shaped closing cap makes the sealing effect better.

进一步,所述进气管和出气管的管径为0.5~1cm。进气管和出气管的管径不宜过大,过大时培养瓶中空气流通过快,选择0.5~1cm的管径为适宜管径。 Further, the diameter of the inlet pipe and the outlet pipe is 0.5-1 cm. The pipe diameter of the inlet pipe and the air outlet pipe should not be too large, and the air flow in the culture bottle will pass quickly when it is too large, and the pipe diameter of 0.5~1cm is selected as the suitable pipe diameter.

进一步,所述弧顶上设有把手。把手的设置利于拿取,方便瓶罩的拆卸和安装。 Further, a handle is provided on the arc top. The setting of the handle is convenient for taking, and it is convenient for the disassembly and installation of the bottle cover.

进一步,所述瓶罩材质为耐高温塑料。耐高温的设置可以直接对瓶罩进行高温杀菌,通过设置不透光和透光的瓶罩,以根据植物是否需要光照来选择合适的瓶罩。 Further, the bottle cover is made of high temperature resistant plastic. The high-temperature-resistant setting can directly sterilize the bottle cover at high temperature. By setting the opaque and light-transmitting bottle cover, the appropriate bottle cover can be selected according to whether the plant needs light.

进一步,所述瓶缸的材质为透光的耐高温玻璃或塑料。耐高温的设置可以直接对瓶缸进行高温杀菌;透光的材质可保证植物得到充足的光照。 Further, the material of the bottle jar is light-transmitting high-temperature-resistant glass or plastic. The high-temperature-resistant setting can directly sterilize the bottle tank at high temperature; the light-transmitting material can ensure that the plants get sufficient light.

进一步,所述瓶缸上设有容量刻度。在倾倒培养基时便于定量。 Further, the bottle cylinder is provided with a capacity scale. Facilitates quantification when pouring media.

进一步,为了缩短培养周期,促进植株生长,向培养瓶内通入二氧化碳,为了节省操作时间和减少培养成本,可以进行多个培养瓶的相互串联,所述第一个培养瓶的进气管连通二氧化碳发生装置,出气管连通第二个培养瓶的进气管,第二个培养瓶的出气管又连通第三个培养瓶的进气管……第n个培养瓶的出气管连通大气,形成一个可高通量培养的过程。 Further, in order to shorten the culture period and promote plant growth, carbon dioxide is introduced into the culture bottle. In order to save operating time and reduce culture costs, multiple culture bottles can be connected in series, and the air inlet pipe of the first culture bottle is connected to the carbon dioxide. Generating device, the air outlet pipe is connected to the air inlet pipe of the second culture bottle, and the air outlet pipe of the second culture bottle is connected to the air inlet pipe of the third culture bottle... The air outlet pipe of the nth culture bottle is connected to the atmosphere, forming a high-speed The process of flux cultivation.

附图说明 Description of drawings

图1为本发明新型的植物组织培养瓶的结构示意图; Fig. 1 is the structural representation of novel plant tissue culture bottle of the present invention;

图2为本发明植物组织培养瓶连续培养的结构示意图。 Fig. 2 is a structural schematic diagram of the continuous cultivation of plant tissue culture bottles of the present invention.

具体实施方式 detailed description

下面通过具体实施方式对本发明作进一步详细的说明: The present invention will be described in further detail below by means of specific embodiments:

说明书附图中的附图标记包括:瓶罩1、微孔滤菌器2、瓶缸3、瓶托4、把手101、弧顶102、进气管103、闭合盖104、瓶身105、出气管106、容量刻度301、外螺纹段302。 The reference signs in the accompanying drawings include: bottle cover 1, microporous bacteria filter 2, bottle cylinder 3, bottle holder 4, handle 101, arc top 102, air inlet pipe 103, closing cap 104, bottle body 105, air outlet pipe 106 , capacity scale 301 , external thread segment 302 .

实施例1:如图1所示:一种新型的植物组织培养瓶,包括相互套接可拆卸的瓶罩1和瓶缸3,瓶罩1包括圆柱形瓶身105以及与瓶身105一体成型的弧顶102,弧顶102的中部连接进气管103,瓶身105上且与弧顶102结合处的下部设有出气管106;瓶缸3的高度为瓶身105高度的1/3倍,所述瓶身105与瓶缸3相对密封,所述瓶缸3的底部连接有瓶托4,瓶身105的下部设有内螺纹段,内螺纹段的上部设有密封圈;所述进气管103和出气管106上均设有微孔滤菌器2,微孔滤菌器2中微孔直径为0.22um;进气管103和出气管106的管口上还连接有圆台形闭合盖104;瓶罩1与瓶缸3相对密封;瓶缸3上设有与内螺纹段啮合的外螺纹段302,瓶缸3上设有容量刻度301。 Embodiment 1: As shown in Figure 1: a new type of plant tissue culture bottle, including a bottle cover 1 and a bottle cylinder 3 that are mutually socketed and detachable, the bottle cover 1 includes a cylindrical bottle body 105 and is integrally formed with the bottle body 105 The arc top 102, the middle part of the arc top 102 is connected to the air inlet pipe 103, and the bottom of the bottle body 105 and the arc top 102 junction is provided with an air outlet pipe 106; the height of the bottle cylinder 3 is 1/3 times of the height of the bottle body 105, The bottle body 105 is relatively sealed with the bottle cylinder 3, the bottom of the bottle cylinder 3 is connected with a bottle holder 4, the bottom of the bottle body 105 is provided with an internal thread section, and the top of the internal thread section is provided with a sealing ring; the air inlet pipe 103 and air outlet pipe 106 are all provided with microporous bacteria filter 2, and the micropore diameter in the microporous bacteria filter 2 is 0.22um; On the mouth of mouth of air inlet pipe 103 and air outlet pipe 106, also be connected with conical closed cover 104; The cover 1 and the cylinder 3 are relatively sealed; the cylinder 3 is provided with an external thread segment 302 engaging with the internal thread segment, and the cylinder 3 is provided with a capacity scale 301 .

如图2所示:第一个培养瓶的进气管103连通二氧化碳发生装置,出气管106连通第二个培养瓶的进气管103,第二个培养瓶的出气管106又连通第三个培养瓶的进气管103……第n个培养瓶的出气管106连通外界,形成一个连续培养过程。 As shown in Figure 2: the air inlet pipe 103 of the first culture bottle communicates with the carbon dioxide generator, the air outlet pipe 106 communicates with the air inlet pipe 103 of the second culture bottle, and the air outlet pipe 106 of the second culture bottle communicates with the third culture bottle The air inlet pipe 103 of the nth culture bottle... the air outlet pipe 106 of the nth culture bottle is connected to the outside world, forming a continuous culture process.

瓶罩1的材质包括透光的耐高温塑料或者不透光的耐高温塑料两种,本实施例选用透光的耐高温塑料;瓶缸3的材质为透光的耐高温玻璃或塑料,本实施例选用透光的耐高温塑料。 The material of the bottle cover 1 includes two kinds of light-transmitting high-temperature-resistant plastics or opaque high-temperature-resistant plastics. The present embodiment selects light-transmitting high-temperature-resistant plastics; The embodiment selects light-transmitting high-temperature-resistant plastics.

实施例2与实施例1的区别在于瓶缸3的高度为瓶身105高度的1/2倍,瓶罩1的材质为不透光的耐高温塑料;瓶缸3的材质为透光的耐高温玻璃。 The difference between embodiment 2 and embodiment 1 is that the height of the bottle cylinder 3 is 1/2 times of the height of the bottle body 105, and the material of the bottle cover 1 is opaque high-temperature-resistant plastic; High temperature glass.

以上所述的仅是本发明的实施例,方案中公知的具体结构及特性等常识在此未作过多描述。应当指出,对于本领域的技术人员来说,在不脱离本发明结构的前提下,还可以作出若干变形和改进,这些也应该视为本发明的保护范围,这些都不会影响本发明实施的效果和专利的实用性。本申请要求的保护范围应当以其权利要求的内容为准,说明书中的具体实施方式等记载可以用于解释权利要求的内容。 What is described above is only an embodiment of the present invention, and common knowledge such as specific structures and characteristics known in the scheme are not described here too much. It should be pointed out that for those skilled in the art, under the premise of not departing from the structure of the present invention, several modifications and improvements can also be made, and these should also be regarded as the protection scope of the present invention, and these will not affect the implementation of the present invention. Effects and utility of patents. The scope of protection required by this application shall be based on the content of the claims, and the specific implementation methods and other records in the specification may be used to interpret the content of the claims.

Claims (9)

1. a novel plant tissue culture bottle, is characterized in that, comprises bottle cover and bottle cylinder, the sealing of described bottle cover is detachably connected on above bottle cylinder, described bottle cover comprises cylindrical body and arc top integrated with body, and the middle part on described arc top is provided with air inlet pipe, and body is provided with escape pipe; The height of bottle cylinder is 1/3 ~ 1/2 of body height, and described bottle cylinder bottom portion is connected with bottle holder.
2. novel plant tissue culture bottle according to claim 1, it is characterized in that: described body is provided with internal thread segment, bottle cylinder is provided with the external thread section engaged with internal thread segment, and the top of internal thread segment is provided with resistant to elevated temperatures sealing ring.
3. novel plant tissue culture bottle according to claim 2, is characterized in that: described air inlet pipe and escape pipe are equipped with dismountable micropore bacteria filter, and in described micropore bacteria filter, micro-pore diameter is 0.22um.
4. novel plant tissue culture bottle according to claim 3, is characterized in that: described air inlet pipe and the mouth of pipe of escape pipe are provided with the truncated cone-shaped closing lid be connected by flexible piece.
5. novel plant tissue culture bottle according to claim 4, is characterized in that: the caliber of described air inlet pipe and escape pipe is 0.5 ~ 1cm.
6. novel plant tissue culture bottle according to claim 5, is characterized in that: described arc top is provided with handle.
7. novel plant tissue culture bottle according to claim 6, is characterized in that: described bottle cover material is high-temperature resistance plastice.
8. novel plant tissue culture bottle according to claim 7, is characterized in that: the material of described bottle cylinder is pyroceram or the plastics of printing opacity.
9. novel plant tissue culture bottle according to claim 8, is characterized in that: described bottle cylinder is provided with containing mark.
CN201510942100.0A 2015-12-16 2015-12-16 Novel plant tissue culture flask Pending CN105494096A (en)

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CN108040878A (en) * 2017-12-25 2018-05-18 上海曼森生物科技有限公司 Tissue culture flasks and the operating method for carrying out tissue cultures
CN115918541A (en) * 2022-12-28 2023-04-07 贵州博盛农业发展有限公司 Macleaya cordata plant tissue culture jar

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CN203692125U (en) * 2013-12-19 2014-07-09 重庆市气象科学研究所 Novel high-luminous-efficiency plant tissue culture flask
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CN104719669A (en) * 2015-03-24 2015-06-24 临沂大学 Preparing and feeding method for chlamys farreri larva baits

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Publication number Priority date Publication date Assignee Title
CN201426296Y (en) * 2008-11-21 2010-03-24 黄永 High-flux culture box of test-tube-free plant tissue cultivated seedling
CN202799770U (en) * 2012-07-25 2013-03-20 中国热带农业科学院热带生物技术研究所 Tissue culture container
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Cited By (3)

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Publication number Priority date Publication date Assignee Title
CN108040878A (en) * 2017-12-25 2018-05-18 上海曼森生物科技有限公司 Tissue culture flasks and the operating method for carrying out tissue cultures
CN115918541A (en) * 2022-12-28 2023-04-07 贵州博盛农业发展有限公司 Macleaya cordata plant tissue culture jar
CN115918541B (en) * 2022-12-28 2023-08-04 贵州博盛农业发展有限公司 A kind of Boluohui plant tissue culture tank

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