CN109628289A - The sampler and method of air miscellaneous bacteria in a kind of detection zymotechnique - Google Patents
The sampler and method of air miscellaneous bacteria in a kind of detection zymotechnique Download PDFInfo
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- CN109628289A CN109628289A CN201910005676.2A CN201910005676A CN109628289A CN 109628289 A CN109628289 A CN 109628289A CN 201910005676 A CN201910005676 A CN 201910005676A CN 109628289 A CN109628289 A CN 109628289A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The invention discloses the samplers and method of air miscellaneous bacteria in a kind of detection zymotechnique, device includes: bottle body, bottle cap, three-way connection, air inlet pipe, exhaust pipe, tampon, sampling pipe, cock, tip side, the end of thread and tongs, utilize the draught head of zymotechnique, sampler and fermentor are directly connected to, directly sampler is placed under corresponding cultivation temperature after sampling and is cultivated, incubation directly can observe the variation of culture in glassware base by vial, film-making microscopy can be carried out to culture medium after reaching cultivation cycle, examine and determine microbiological contamination type.This method can be sampled by the simplest equipment, easy to operate, and can carry out continuous sampling for a long time according to actual needs, and the representativeness of institute's sample thief will not be influenced because of the fluctuation of air system, as a result can more react the aseptic condition of air system.
Description
Technical field
The present invention relates to a kind of samplers and method for detecting miscellaneous bacteria, and in particular to air in a kind of detection zymotechnique
The sampler and method of miscellaneous bacteria, belong to microorganism detection field.
Background technique
In modern industry fermentation process, either solid state fermentation or liquid state fermentation can all use filtrated air, to meet
The needs of microorganism growth, especially in oxygen consumption type fermenting and producing, plant air needs to guarantee rigorous aseptic, generally by air compressor machine
Group carries out compression heating to go miscellaneous bacteria for the first time, recycles pre-, thick, smart air filter to be allowed to reach after reaching each workshop
Consumption to sterile grade, air is very big, and microbiological contamination is the number one killer of fermentation, carries disease germs and will certainly give birth to fermentation once air occurs
Production causes extreme influence, therefore how to guarantee that the quality of filtrated air is just particularly important.Currently, in laboratory or industry
It is all to be passed through the air of sampling at high altitude in fermentor after special filter element filtering to use during changing, it periodically can be to filter core
It is sterilized with air pipeline to guarantee the quality of filtrated air, is not related to that filtrated air sample is taken to carry out microorganism point therebetween
Analysis.
Summary of the invention
In view of this, the present invention provides the samplers and method of air miscellaneous bacteria in a kind of detection zymotechnique, not only
Sampler simple structure, sampling method operation is easy, and crucial sustainable sampling, result is more standby representative, is more suitable for
Fermentation actual production can also carry out culture medium replacement according to practical, achieve the purpose that targetedly to detect.
To achieve the goals above, the present invention adopts the following technical scheme:
The sampler of air miscellaneous bacteria in a kind of detection zymotechnique, comprising:
Bottle body；
Bottle cap, bottle cap are adapted to connection with the bottleneck of bottle body, and bottle cap is through its upper and lower surface and offers through-hole；
Three-way connection, three-way connection include connector one, connector two and the connector three for the tubulose for being integrally formed and being interconnected,
Connector one is adapted to through-hole, and one end insertion through-hole simultaneously extends in bottle body；
Air inlet pipe, air inlet pipe are set in bottle body and are connected to one end of connector one；
Exhaust pipe, exhaust pipe one end are closely connect with connector two；
The other end of exhaust pipe is arranged in tampon, tampon；
Sampling pipe, sampling pipe one end and connector tee joint；
Cock, cock middle part are equipped with the channel for extending vertically through its end face, and one end of cock is tip side, and the other end is screw thread
End, the outer wall of tip side are equipped with the non-slip groove equidistantly arranged, and tip side is inserted into the other end of sampling pipe；
With, tongs, tongs are arranged on exhaust pipe and sampling pipe.
The present invention detects the sampler simple in structure, good airproof performance of air miscellaneous bacteria in zymotechnique, strong interference immunity, benefit
Be that barotropic state is sampled with zymotechnique air, without adding air-extractor, and will not due to the fluctuation of air system shadow
The representativeness for ringing institute's sample thief is more suitable for fermentation actual production.
Further, contain fluid nutrient medium in bottle body.
It is using above-mentioned further beneficial effect, enrichment bacterium can targetedly be cultivated by being sampled using culture medium
Kind, simple, intuitive and accurately can judge in air whether to have miscellaneous bacteria and identify miscellaneous bacteria by adjusting the type of culture medium
Type.
The present invention also provides the sampling methods of air miscellaneous bacteria in detection zymotechnique, comprising the following steps:
1) it is assembled after being respectively washed each component of above-mentioned sampler, and fluid nutrient medium is added in bottle body；
2) the cock end of thread is packed tightly with double gauze, outer layer is wrapped up in the double-deck brown paper again and tightened with cotton thread, with gauze packet
After close-up tracheae is equipped with one end of tampon, sampler is placed in sterilizing cabinet and is sterilized, 120-124 DEG C of sterilising temp, time 25-
35min, pressure 0.10-0.14MPa；
3) it when the intracorporal culture medium of bottle naturally cools to 35-40 DEG C, closes fermentor and is vented mouthpiece ball valve, with 75%
Alcohol removes the brown paper of the end of thread after sanitized to carrying out disinfection inside ball valve and mouthpiece, by the end of thread close to exhaust
Gauze is slowly opened after mouthpiece ball valve and screws the end of thread and exhaust mouthpiece ball valve, slowly removes tongs and the slowly row of opening
To there is bubble to be discharged by air inlet pipe, the record time simultaneously starts to sample, sampling time 48-72h, preferably 48h air-blowing mouth ball valve,
It can adjust according to the actual situation；
4) exhaust mouthpiece ball valve is first closed after sampling, then by spiral shell after tongs are installed to exhaust pipe and sampling pipe
Line end is separated with exhaust mouthpiece ball valve, and sampler is transferred in incubator, is removed after the tongs on exhaust pipe to sampling
Culture medium is cultivated in device, and the time of culture is 60-84h, and preferably 72h, temperature is 35-37 DEG C, preferably 37 DEG C, can root
It is adjusted according to actual conditions, film-making and microscopy, see whether microbiological contamination, and judge bacterial type after culture.
Detection method operating process of the invention is extremely easy, after being connect with sample point mouthpiece can long-term and stabilization adopt
Sample, sampled result more persuasion property, is not necessarily to operation bidirectional, complete equipment is placed directly under corresponding cultivation temperature after sampling
It is cultivated, incubation directly can observe the variation of culture in glassware base by vial, and reaching can be to culture medium after cultivation cycle
Film-making microscopy is carried out, microbiological contamination type, testing result high reliablity are examined and determine.
Further, sampling further includes sampling the operation of inspection in step 3), and the operation for sampling inspection is every 10-12h, preferably
The rate uniform of exhaust mouthpiece ball valve capacity to air inlet pipe discharge bubble is adjusted for 12h.
It is using above-mentioned further beneficial effect, air system is fluctuation, and adjustment air-flow size is allowed to uniformly adopt
Sample, it is possible to prevente effectively from error, guarantees that testing result is accurate, it is with a high credibility.
Further, the operation cleaned in step 1) is that each component sodium hydroxide solution of sampler is impregnated 30-40min
It is rinsed well afterwards with tap water, uses distilled water rinse, rinse to rinse liquid pH=7.0-8.0 later.
It is using above-mentioned further beneficial effect, sufficiently cleaning sampler, can effectively guarantees experimental provision
Initial cleanliness, on the one hand will not influence the property of culture medium, another accuracy for conveniently improving testing result and credible
Degree.
Further, the additional amount of fluid nutrient medium is that the 40%- for being full of bottle body is added to fluid nutrient medium in step 1)
70%, fluid nutrient medium is the phenol red meat soup of glucose, can replace other fluid nutrient mediums according to actual needs.
It is using above-mentioned further beneficial effect, the phenol red meat soup of glucose (beef extract 0.3%, peptone 0.8%,
Glucose 0.5%, sodium chloride 0.5%, 1% phenol red solution 0.4%, pH7.2) it is able to satisfy the nutrition in various bacteria growth course
Demand, in bacteria growth process, glucose fermentation generate acid and make it is phenol red turned yellow by red, preparation method is simple, at
This is controllable, and visual result easily determines.
Further, the operation screwed the end of thread and exhaust mouthpiece ball valve in step 3) further includes using cotton ball soaked in alcohol after screwing
Junction is sterilized and is packed tightly with gauze, brown paper and cotton thread.
It is using above-mentioned further beneficial effect, deadend contact position, avoids generating caused by operation error
Gap and make the bacterium in air enter sampler influence testing result, utmostly guarantee that strain collected is all from hair
Fermentation tank guarantees testing result accuracy.
Detailed description of the invention
Fig. 1 is the structure chart of the sampler of air miscellaneous bacteria in present invention detection zymotechnique；
In attached drawing, parts list represented by the reference numerals are as follows:
1, bottle body；2, bottle cap；3, three-way connection；4, air inlet pipe；5, exhaust pipe；6, tampon；7, sampling pipe；8, cock；81,
Tip side；82, the end of thread；9, tongs.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the examples below, used sampler structure such as Fig. 1, comprising:
Bottle body 1；
Bottle cap 2, bottle cap 2 are adapted to connection with the bottleneck of bottle body 1, and bottle cap 2 is through its upper and lower surface and offers through-hole；
Three-way connection 3, three-way connection 3 include connector one, connector two and the connector for the tubulose for being integrally formed and being interconnected
Three, connector one is adapted to through-hole, and one end insertion through-hole simultaneously extends in bottle body 1；
Air inlet pipe 4, air inlet pipe 4 are set in bottle body 1 and are connected to one end of connector one；
Exhaust pipe 5,5 one end of exhaust pipe are closely connect with connector two；
The other end of exhaust pipe 5 is arranged in tampon 6, tampon 6；
Sampling pipe 7,7 one end of sampling pipe and connector tee joint；
Cock 8, the channel for extending vertically through its end face is equipped in the middle part of cock 8, and one end of cock 8 is tip side 81, the other end
For the end of thread 82, the outer wall of tip side 81 is equipped with the non-slip groove equidistantly arranged, and tip side 81 is inserted into the another of sampling pipe 7
End；
With, tongs 9, tongs 9 are arranged on exhaust pipe 5 and sampling pipe 7.
Set culture medium is the phenol red meat soup of glucose in sampler.
Embodiment 1
Detect the sampling method of air miscellaneous bacteria in zymotechnique, comprising the following steps:
1) it is rinsed well after each component of sampler being impregnated 30min with sodium hydroxide solution with tap water, later with steaming
Distilled water rinse assembles after rinse to rinse liquid pH=7.0, and fluid nutrient medium to fluid nutrient medium is added in bottle body and is full of bottle
The 40% of body；
2) the cock end of thread is packed tightly with double gauze, outer layer is wrapped up in the double-deck brown paper again and tightened with cotton thread, with gauze packet
After close-up tracheae is equipped with one end of tampon, sampler is placed in sterilizing cabinet and is sterilized, 120 DEG C of sterilising temp, time 25min,
Pressure 0.10MPa；
3) it when the intracorporal culture medium of bottle naturally cools to 35 DEG C, closes fermentor and is vented mouthpiece ball valve, with 75% wine
Essence removes the brown paper of the end of thread after sanitized to carrying out disinfection inside ball valve and mouthpiece, and the end of thread is blown close to exhaust
Gauze is slowly opened after mouth ball valve and screws the end of thread and exhaust mouthpiece ball valve, is sterilized junction with cotton ball soaked in alcohol after screwing
And packed tightly with gauze, brown paper and cotton thread, slowly remove tongs and slowly open exhaust mouthpiece ball valve to have bubble by into
Tracheae discharge, record the time and start sampling and every 10h adjustment exhaust mouthpiece ball valve capacity to air inlet pipe discharge bubble speed
Rate is uniform, sampling time 48h；
4) exhaust mouthpiece ball valve is first closed after sampling, then by spiral shell after tongs are installed to exhaust pipe and sampling pipe
Line end is separated with exhaust mouthpiece ball valve, and sampler is transferred in incubator, and the tongs removed on exhaust pipe are cultivated,
The time of culture is 60h, and temperature is 35 DEG C, and film-making and microscopy, see whether microbiological contamination, and judge bacterial type after culture.
Embodiment 2
Detect the sampling method of air miscellaneous bacteria in zymotechnique, comprising the following steps:
1) it is rinsed well after each component of sampler being impregnated 40min with sodium hydroxide solution with tap water, later with steaming
Distilled water rinse assembles after rinse to rinse liquid pH=8.0, and fluid nutrient medium to fluid nutrient medium is added in bottle body and is full of bottle
The 70% of body；
2) the cock end of thread is packed tightly with double gauze, outer layer is wrapped up in the double-deck brown paper again and tightened with cotton thread, with gauze packet
After close-up tracheae is equipped with one end of tampon, sampler is placed in sterilizing cabinet and is sterilized, 124 DEG C of sterilising temp, time 35min,
Pressure 0.14MPa；
3) it when the intracorporal culture medium of bottle naturally cools to 40 DEG C, closes fermentor and is vented mouthpiece ball valve, with 75% wine
Essence removes the brown paper of the end of thread after sanitized to carrying out disinfection inside ball valve and mouthpiece, and the end of thread is blown close to exhaust
Gauze is slowly opened after mouth ball valve and screws the end of thread and exhaust mouthpiece ball valve, is sterilized junction with cotton ball soaked in alcohol after screwing
And packed tightly with gauze, brown paper and cotton thread, slowly remove tongs and slowly open exhaust mouthpiece ball valve to have bubble by into
Tracheae discharge, record the time and start sampling and every 12h adjustment exhaust mouthpiece ball valve capacity to air inlet pipe discharge bubble speed
Rate is uniform, sampling time 72h；
4) exhaust mouthpiece ball valve is first closed after sampling, then by spiral shell after tongs are installed to exhaust pipe and sampling pipe
Line end is separated with exhaust mouthpiece ball valve, and sampler is transferred in incubator, and the tongs removed on exhaust pipe are cultivated,
The time of culture is 84h, and temperature is 37 DEG C, and film-making and microscopy, see whether microbiological contamination, and judge bacterial type after culture.
Embodiment 3
Detect the sampling method of air miscellaneous bacteria in zymotechnique, comprising the following steps:
1) it is rinsed well after each component of sampler being impregnated 30min with sodium hydroxide solution with tap water, later with steaming
Distilled water rinse assembles after rinse to rinse liquid pH=7.5, and fluid nutrient medium to fluid nutrient medium is added in bottle body and is full of bottle
The 50% of body；
2) the cock end of thread is packed tightly with double gauze, outer layer is wrapped up in the double-deck brown paper again and tightened with cotton thread, with gauze packet
After close-up tracheae is equipped with one end of tampon, sampler is placed in sterilizing cabinet and is sterilized, 120 DEG C of sterilising temp, time 30min,
Pressure 0.10MPa；
3) it when the intracorporal culture medium of bottle naturally cools to 35 DEG C, closes fermentor and is vented mouthpiece ball valve, with 75% wine
Essence removes the brown paper of the end of thread after sanitized to carrying out disinfection inside ball valve and mouthpiece, and the end of thread is blown close to exhaust
Gauze is slowly opened after mouth ball valve and screws the end of thread and exhaust mouthpiece ball valve, is sterilized junction with cotton ball soaked in alcohol after screwing
And packed tightly with gauze, brown paper and cotton thread, slowly remove tongs and slowly open exhaust mouthpiece ball valve to have bubble by into
Tracheae discharge, record the time and start sampling and every 12h adjustment exhaust mouthpiece ball valve capacity to air inlet pipe discharge bubble speed
Rate is uniform, sampling time 48h；
4) exhaust mouthpiece ball valve is first closed after sampling, then by spiral shell after tongs are installed to exhaust pipe and sampling pipe
Line end is separated with exhaust mouthpiece ball valve, and sampler is transferred in incubator, and the tongs removed on exhaust pipe are cultivated,
The time of culture is 72h, and temperature is 37 DEG C, and film-making and microscopy, see whether microbiological contamination, and judge bacterial type after culture.
Detecting appraisal
The culture medium after embodiment 1-3 culture is subjected to film-making respectively, each culture medium makes 3 smears respectively and divides
Not carry out microscopy, acquired results such as table 1.
1 embodiment 1-3 sample smear for microscopic examination result of table
By the result in table 1 it is found that the present invention can intuitively accurate reaction detection as a result, the testing result measured is straight
It sees, accuracy is high, representative.
Claims (10)
1. the sampler of air miscellaneous bacteria in a kind of detection zymotechnique characterized by comprising
Bottle body (1)；
Bottle cap (2), the bottle cap (2) are adapted to connection with the bottleneck of the bottle body (1), and the bottle cap (2) runs through its upper and lower surface
And offer through-hole；
Three-way connection (3), the three-way connection (3) include connector one, two and of connector for the tubulose for being integrally formed and being interconnected
Connector three, the connector one are adapted to the through-hole, and one end is inserted into the through-hole and is extended in the bottle body (1)；
Air inlet pipe (4), the air inlet pipe (4) are set in the bottle body (1) and are connected to one end of the connector one；
Exhaust pipe (5), described exhaust pipe (5) one end are closely connect with the connector two；
Tampon (6), the other end of tampon (6) setting in the exhaust pipe (5)；
Sampling pipe (7), described sampling pipe (7) one end and the connector tee joint；
Cock (8), cock (8) middle part are equipped with the channel for extending vertically through its end face, and one end of the cock (8) is tip side
(81), the other end is the end of thread (82), and the outer wall of the tip side (81) is equipped with non-slip groove, and the tip side (81) is inserted into institute
State the other end of sampling pipe (7)；
With, tongs (9), the tongs (9) are arranged on the exhaust pipe (5) and the sampling pipe (7).
2. the sampler of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 1, which is characterized in that described
Non-slip groove spaced set on tip side (81) outer wall.
3. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique, which comprises the following steps:
1) it is assembled after being respectively washed each component of sampler of any of claims 1 or 2, and liquid is added in bottle body (1)
Culture medium；
2) end of thread (82) of the cock (8) being packed tightly with double gauze, outer layer is wrapped up in the double-deck brown paper again and is tightened with cotton thread,
After packing tightly the exhaust pipe (5) equipped with the one end of tampon (6) with gauze, the sampler is placed in sterilizing cabinet and is sterilized；
3) after sterilizing, when the culture medium in the bottle body (1) naturally cools to 35-40 DEG C, the exhaust mouthpiece of fermentor is closed
Ball valve takes the brown paper of the end of thread (82) after sanitized with alcohol to carrying out disinfection inside the ball valve and mouthpiece
Under, will the end of thread (82) slowly open after described exhaust mouthpiece ball valve gauze and by the end of thread (82) with it is described
Exhaust mouthpiece ball valve screws, and slowly removes the tongs (9) and slowly opens the exhaust mouthpiece ball valve to there is bubble to pass through
Air inlet pipe (4) discharge, records the time and starts to sample；
4) first close the exhaust mouthpiece ball valve after sampling, then the tongs (9) are installed to the exhaust pipe (5) and
The end of thread (82) is separated with the exhaust mouthpiece ball valve after on the sampling pipe (7), the sampler is transferred to
In incubator, the culture medium in the sampler is cultivated after removing the tongs (9) on the exhaust pipe (5), is trained
Film-making and microscopy, see whether microbiological contamination, and judge bacterial type after supporting.
4. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 3, which is characterized in that described
Starting sampling in step 3) further includes sampling the operation of inspection, and the operation of the sampling inspection is that every 10-12h adjusts the exhaust
The rate uniform of bubble is discharged to the air inlet pipe (4) for mouthpiece ball valve capacity.
5. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 3, which is characterized in that described
The operation cleaned in step 1) is to use tap water after each component of the sampler is impregnated 30-40min with sodium hydroxide solution
It rinses well, later with distilled water rinse to rinse liquid pH=7.0-8.0.
6. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 3, which is characterized in that step
1) additional amount of fluid nutrient medium described in is that the 40%-70% for being full of the bottle body (1) is added to fluid nutrient medium.
7. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 3, which is characterized in that step
2) condition of sterilizing described in is 120-124 DEG C of temperature, time 25-35min, pressure 0.10-0.14MPa.
8. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 3, which is characterized in that step
3) operation for screwing the end of thread (82) with the exhaust mouthpiece ball valve in further includes that will be connect after screwing with cotton ball soaked in alcohol
Place sterilizes and is packed tightly with gauze, brown paper and cotton thread.
9. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 3, which is characterized in that step
3) sampling time 48-72h described in.
10. the sampling method of air miscellaneous bacteria in a kind of detection zymotechnique according to claim 3, which is characterized in that step
It is rapid 4) described in culture time be 60-84h, temperature be 35-37 DEG C.
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| CN113637567A (en) * | 2021-06-29 | 2021-11-12 | 安徽曦强乳业集团有限公司 | Production is with miscellaneous fungus detection device of fermented milk sampling |
| CN115824616A (en) * | 2023-02-13 | 2023-03-21 | 潍坊格菲特生物科技有限公司 | On-line integrity test method for filter element of fine filter |
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| CN113637567A (en) * | 2021-06-29 | 2021-11-12 | 安徽曦强乳业集团有限公司 | Production is with miscellaneous fungus detection device of fermented milk sampling |
| CN115824616A (en) * | 2023-02-13 | 2023-03-21 | 潍坊格菲特生物科技有限公司 | On-line integrity test method for filter element of fine filter |
| CN115824616B (en) * | 2023-02-13 | 2023-04-14 | 潍坊格菲特生物科技有限公司 | On-line integrity test method for filter element of fine filter |
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Application publication date: 20190416 |