CN208980721U - The outer pH real-time detecting system of fermentor tank - Google Patents
The outer pH real-time detecting system of fermentor tank Download PDFInfo
- Publication number
- CN208980721U CN208980721U CN201821407028.7U CN201821407028U CN208980721U CN 208980721 U CN208980721 U CN 208980721U CN 201821407028 U CN201821407028 U CN 201821407028U CN 208980721 U CN208980721 U CN 208980721U
- Authority
- CN
- China
- Prior art keywords
- electrode
- fermentor
- container
- fermentation
- stainless steel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model patent discloses the pH real-time detecting system outside a set of fermentor tank, which is made of syringe needle, silicone tube, peristaltic pump, pH electrode, pH electrode stainless steel sheath, sealing ring, the rustless steel container with screw thread mouth, pagoda connector, nut, gasket；The set system flows back into fermentation system after carrying out pH detection in external bactericidal unit using the fermentation liquid in wriggling pump pressure extraction fermentor, does not bring fermentation liquid to lose, while preventing fermentation liquid contaminated by sophisticated design；The pH real-time detection to fermentation cylinder for fermentation liquid is not only realized, the delay and fermentor microbiological contamination risk of frequent manual sampling to external detection are avoided；And the problems such as changing in former traditional zymotic mode, pH electrode direct-insert is entered to need to be subjected in fermentor fermentor autoclave sterilization bring ageing of electrode, structure destruction, the service life of pH electrode is extended, and ensure that the stabilization of fermentation system；Meanwhile the set system cost is low, operating effect is good, and controllability is strong, easily operated.
Description
Technical field
It is real-time that the utility model patent is related to the outer pH value of a kind of detection system, the especially tank of biofermentation industry fermentor
Detection system.
Background technique
In biofermentation production, need to detect the pH value of fermentation liquid at any time, to determine the cell state in fermentation process, and
PH value detected also needs to realize the automation control to fermentation system as the basic parameter factor.But because of biological fermentation process
In need to maintain germ-free condition always, therefore before starting fermentation, need to carry out high-temperature sterilization to entire fermentor.And pH electrode due to
It needs to contact with fermentation liquid, so needing just to be installed in place before fermentation starts, and carries out high-temperature steam together with culture medium
Sterilizing.But the environment of high temperature and pressure is very big for the service life influence of pH electrode, pH electrode easily occurs aging and influences
Accuracy in detection, and pH electrode price is extremely expensive, continuous replacement has a huge impact the cost control of fermenting and producing.And
To improve the service life of pH electrode, then pH electrode, which cannot then be inserted into fermentor, carries out in situ detection, currently used side
Method is at regular intervals, a part of sample to be taken out, in the pH value of externally measured fermented sample.But there are three lack for this method
Point can not continuously detect pH value first is that needing to detect sample every a period of time, once fermentation liquid pH occurs
Mutation, can not find and intervene in time；Second is that will also result in loss to sample due to wanting continuous sampling；Third is that continuous sampling
Also a possibility that will increase fermentor pollution.
Summary of the invention
It is easily destroyed by high temperature and high pressure steam for the pH electrode of current insertion fermentation tank, influences asking for service life
Topic, provided new system can either continuously detect pH value, and not will cause the loss of sample and the pollution of fermentor,
And on pH electrode life without influence.
The outer pH value of a set of fermentor tank for being suitable for carrying out fermentation process detection that the utility model patent provides is examined in real time
Examining system, the system by syringe needle, silicone tube, peristaltic pump, pH electrode, pH electrode stainless steel sheath, sealing ring, with screw thread mouth
Rustless steel container, pagoda connector, nut, gasket composition.
Wherein,
Needle diameter is 8mm, and needle length 50mm, silica gel length of tube is 1-2m, and silica gel bore is 6.4mm, outer diameter
For 9.6mm.
PH electrode matches with pH electrode stainless steel protection set, and pH electrode stainless steel protection set has screw buckle, screw buckle
Size matches with rustless steel container screw thread mouth size.
PH electrode output signal simultaneously connects fermentor control system.
Sealing ring size matches with stainless steel electrode protective case, covers on stainless steel electrode protective case, guarantees sealing effect
Fruit, not microbiological contamination.
Rustless steel container diameter is 60mm-80mm, and total height 140mm, there are screw thread mouth, diameter 40mm- in top center
60mm, height 20mm.And inlet and liquid outlet are had, inlet and liquid outlet are tightened with pagoda connector with nut, and are installed
Gasket guarantees the tightness of container, and inlet is in container lower end, and apart from container bottom end 20mm, liquid outlet is in container upper end, distance
Container bottom end 90mm.
Silicone tube and rustless steel container connects using pagoda connector, and wherein pagoda connector uses 4 exceptionally silk * 8mm types, overall length
Spend 35mm.
Peristaltic pump uses accurate peristaltic pump, and power 30W has start-up and shut-down control and speed controlling function, and the range of speeds is
0.1rpm-100rpm, by pressing key control, reference flow 266.7mL/min.
Application method
Two thick connectors of pagoda are connected on the inlet and liquid outlet of rustless steel container with nut and gasket, so
Two silicone tubes are connected respectively on two pagoda connectors afterwards, two syringe needles are connected to the silicone tube other end, use ox-hide
Paper bag lives the screw top mouth of rustless steel container, the package unit connected is placed in a big stainless steel box, after covering box
It is put into high-pressure sterilizing pot and sterilizes, 121 DEG C of sterilising temp, sterilize 30min.Meanwhile the stainless steel protection set of pH electrode being put on
Sealing ring, is wrapped and aforementioned stainless steel single unit system autoclave sterilization together with brown paper.After sterilizing, by stainless steel entirety
Device and pH electrode stainless steel protection set (containing screw buckle) are put into togerther in superclean bench, on superclean bench, open work
Operation mode is installed to pH electrode stainless steel protection after wiping the pH electrode after cleaning, calibration with alcohol swab and puts on, by pH electrode
It is inserted into and in the screw thread mouth that is installed at the top of rustless steel container, the height of bottom end is identical as lower liquid outlet.Whole system installation
After good, the operating mode of superclean bench is closed, opens ultraviolet lamp, ultraviolet irradiation 30min carries out sterilization again.Because
Primary fermentation tank is inserted directly into pH electrode and carries out pH detection, therefore generally has the insertion of a pH electrode in fermentor lower end
Mouthful, this mouth is clogged with silica gel plug, and sterilizes together with fermentor and culture solution therein, end subject to sterilization is formally sent out
When ferment, silica gel outer surface is wiped, and quick insertion syringe needle with alcohol, connects fermentation liquid and fermentor tank by this method
The silicone tube of liquid outlet is simultaneously inserted on the silica gel plug of fermentor charging aperture by outer pH detection system, with peristaltic pump band
The silicone tube of dynamic inlet is expelled back into fermentor after external pH detection again so that fermentation liquid is constantly discharged from fermentor,
It realizes the outer pH real-time detection of fermentor tank, and guarantees whole system not microbiological contamination, realize the protection to pH electrode, avoid pH electricity
The quick aging of pole.
The utility model patent mainly has the advantages that
The outer pH value real-time detection apparatus of the fermentor tank of above-mentioned design not only realizes real to the pH of fermentation cylinder for fermentation liquid
When detect, avoid delay of the frequent manual sampling to external detection and the pollution to fermentor；And change former tradition hair
In ferment mode, pH electrode direct-insert is entered to need to be subjected in fermentor fermentor autoclave sterilization bring ageing of electrode, structure is broken
Bad the problems such as, the service life of pH electrode is extended, and ensure that fermentation system not and will receive pollution.Meanwhile the set system at
This is low, and operating effect is good, and controllability is strong, easily operated.
Detailed description of the invention
Attached drawing 1 is the structural schematic diagram of system.
Specific embodiment
Embodiment 1
By two pagoda connectors (1；2) thick head nut and gasket are connected to the inlet (4) of rustless steel container (3)
On liquid outlet (5), then by two silicone tubes (6；7) it is connected respectively on two pagoda connectors, two syringe needles are separately connected
In the other end of silicone tube, the screw top mouth of rustless steel container is encased with brown paper, the package unit connected is placed in greatly
Stainless steel box, cover to be put into high-pressure sterilizing pot after box and sterilize, 121 DEG C of sterilising temp, sterilize 30min.Meanwhile by pH electrode
Stainless steel protection set (8) cover upper seal, wrapped with brown paper and aforementioned stainless steel single unit system high temperature and pressure is gone out together
Bacterium.After sterilizing, stainless steel single unit system and pH electrode stainless steel protection set are put into togerther in superclean bench, in ultra-clean work
On platform, operating mode is opened, pH electrode stainless steel is installed to after the pH electrode (9) after cleaning, calibration is wiped with alcohol swab and protects
On sheath, pH electrode is inserted into and in the screw thread mouth that is installed at the top of rustless steel container, height and the lower liquid outlet phase of bottom end
Together.After whole system installs, close the operating mode of superclean bench, open ultraviolet lamp, ultraviolet irradiation 30min, again into
Row sterilization.Because primary fermentation tank is inserted directly into pH electrode and carries out pH detection, generally have one in fermentor lower end
This mouth is clogged with silica gel plug, and sterilized together with fermentor and culture solution therein by the insert port of a pH electrode, knot subject to sterilization
Beam when formally being fermented, silica gel outer surface is wiped, and quick insertion syringe needle 1 (10) with alcohol, is connected by this method
The silicone tube syringe needle 2 (11) of liquid outlet is simultaneously inserted into fermentor by sending and receiving zymotic fluid and the outer pH detection system of fermentor tank
On the silica gel plug of charging aperture, the silicone tube of inlet is driven with peristaltic pump (12), so that fermentation liquid is constantly discharged from fermentor,
It is expelled back into fermentor again after external pH detection, realizes the outer pH real-time detection of fermentor tank, and guarantee whole system not microbiological contamination,
The protection to pH electrode is realized, the quick aging of pH electrode is avoided.
It should be noted that it is above it is described be specific embodiment of the utility model and the technical principle used,
If changing made by the conception according to the utility model, what generated function was still covered without departing from specification and attached drawing
When spiritual, the protection scope of the utility model should be belonged to.
Claims (2)
1. a set of outer pH value real-time detecting system of the fermentor tank for being suitable for carrying out fermentation process detection, which is characterized in that this is
System is by syringe needle, silicone tube, peristaltic pump, pH electrode, pH electrode stainless steel sheath, sealing ring, rustless steel container, pagoda connector, spiral shell
Female, gasket composition；Two pagoda thick connector nuts and gasket are connected to the inlet and liquid outlet of rustless steel container
On, then two silicone tubes are connected respectively on two pagoda connectors, two syringe needles are connected to the silicone tube other end；It will
The stainless steel protection set set upper seal of pH electrode, is installed to pH electricity after the pH electrode after cleaning, calibration is wiped with alcohol swab
Pole stainless steel protection is put on, and pH electrode is inserted into and in the screw thread mouth that is installed at the top of rustless steel container；With peristaltic pump drive into
The silicone tube of liquid mouth, so that fermentation liquid is constantly discharged from fermentor.
2. the outer pH value real-time detection system of a set of fermentor tank for being suitable for carrying out fermentation process detection according to claim 1
System, which is characterized in that the rustless steel container diameter is 60mm-80mm, total height 140mm, and there is screw thread mouth in top center, directly
Diameter is 40mm-60mm, height 20mm, and has inlet and liquid outlet, and inlet is in container lower end, apart from container bottom end
20mm, liquid outlet is in container upper end, apart from container bottom end 90mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821407028.7U CN208980721U (en) | 2018-08-29 | 2018-08-29 | The outer pH real-time detecting system of fermentor tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821407028.7U CN208980721U (en) | 2018-08-29 | 2018-08-29 | The outer pH real-time detecting system of fermentor tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208980721U true CN208980721U (en) | 2019-06-14 |
Family
ID=66782670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821407028.7U Expired - Fee Related CN208980721U (en) | 2018-08-29 | 2018-08-29 | The outer pH real-time detecting system of fermentor tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208980721U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772296A (en) * | 2021-03-09 | 2021-05-11 | 贵州贵旺生物科技有限公司 | Liquid strain culture device and culture method for pleurotus eryngii |
| CN114686367A (en) * | 2022-05-06 | 2022-07-01 | 白银赛诺生物科技有限公司 | Electrode protection device for fermentation environment, use method and calibration method |
-
2018
- 2018-08-29 CN CN201821407028.7U patent/CN208980721U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772296A (en) * | 2021-03-09 | 2021-05-11 | 贵州贵旺生物科技有限公司 | Liquid strain culture device and culture method for pleurotus eryngii |
| CN112772296B (en) * | 2021-03-09 | 2023-07-04 | 贵州贵旺生物科技有限公司 | Liquid strain culture device and culture method for pleurotus eryngii |
| CN114686367A (en) * | 2022-05-06 | 2022-07-01 | 白银赛诺生物科技有限公司 | Electrode protection device for fermentation environment, use method and calibration method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208980721U (en) | The outer pH real-time detecting system of fermentor tank | |
| CN206828536U (en) | A kind of coliform detection means | |
| CN201756550U (en) | Fully-sealed bacteria collection ampoule incubator | |
| CN107216996A (en) | A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation | |
| CN202279822U (en) | Sampler of dislocated disinfection bioreactor | |
| CN205347433U (en) | Kitchen garbage digest anaerobic fermentation device | |
| CN211814381U (en) | Microbial agent production line | |
| CN210963154U (en) | Movable vertical sterilizer | |
| CN209368236U (en) | A kind of quick subpackage apparatus of anaerobism solid-liquid culture medium | |
| CN209630221U (en) | A kind of sterilization tank of Multiple electrolytes injection production | |
| CN207193279U (en) | A kind of culture transferring bottle | |
| CN207330923U (en) | A kind of microorganism produces the collection device of gas | |
| CN202568971U (en) | Process challenge device capable of double-end monitoring | |
| CN208121101U (en) | Small-sized fermentation tank | |
| CN204661695U (en) | A kind of sampling unit detecting fermentative production sterile air | |
| CN205313561U (en) | A fermentation cylinder for microorganism continuous culture | |
| CN217202706U (en) | Safe convenient fermentation cylinder feed supplement device | |
| CN211921479U (en) | Inoculator for refined brewing beer | |
| CN219460244U (en) | Full-automatic milk disinfection and sterilization device | |
| CN201399103Y (en) | Monitoring device with challenged low-temperature vacuum formaldehyde sterilization effect | |
| CN208036744U (en) | A kind of attachment device being suitable for adding sensitive materials | |
| CN221071484U (en) | Liquid fermentation tank for traditional Chinese medicine ferment | |
| CN216645742U (en) | Microorganism challenge test device | |
| CN219950943U (en) | Filter equipment is used in white spirit food safety inspection | |
| CN216639446U (en) | Biological fermentation tank and tail gas treatment device thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190614 Termination date: 20200829 |